subject:(zebrafish)

University of Georgia

1. Moss, Lauren Dianne. The zebrafish coelomic cavity: a model tissue for studies of innate and adaptive immunity.

Degree: MS, Infectious Diseases, 2008, University of Georgia

URL: http://purl.galileo.usg.edu/uga_etd/moss_lauren_d_200805_ms

► The zebrafish (Danio rerio) is an emerging model in immunological and developmental studies. One of the practical limitations in this model is the ability to… (more)

Subjects/Keywords: zebrafish

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APA (6^th Edition):

Moss, L. D. (2008). The zebrafish coelomic cavity: a model tissue for studies of innate and adaptive immunity. (Masters Thesis). University of Georgia. Retrieved from http://purl.galileo.usg.edu/uga_etd/moss_lauren_d_200805_ms

Chicago Manual of Style (16^th Edition):

Moss, Lauren Dianne. “The zebrafish coelomic cavity: a model tissue for studies of innate and adaptive immunity.” 2008. Masters Thesis, University of Georgia. Accessed July 19, 2019. http://purl.galileo.usg.edu/uga_etd/moss_lauren_d_200805_ms.

MLA Handbook (7^th Edition):

Moss, Lauren Dianne. “The zebrafish coelomic cavity: a model tissue for studies of innate and adaptive immunity.” 2008. Web. 19 Jul 2019.

Vancouver:

Moss LD. The zebrafish coelomic cavity: a model tissue for studies of innate and adaptive immunity. [Internet] [Masters thesis]. University of Georgia; 2008. [cited 2019 Jul 19]. Available from: http://purl.galileo.usg.edu/uga_etd/moss_lauren_d_200805_ms.

Council of Science Editors:

Moss LD. The zebrafish coelomic cavity: a model tissue for studies of innate and adaptive immunity. [Masters Thesis]. University of Georgia; 2008. Available from: http://purl.galileo.usg.edu/uga_etd/moss_lauren_d_200805_ms

Oregon State University

2. Norris, Lauren J. Controlling Important Pathogens in Zebrafish (Danio rerio): Assessing Cryopreservation Survival of Bacteria and Parasites and Clinical Sensitivity of Mycobacterial qPCR Assays.

Degree: MS, 2017, Oregon State University

URL: http://hdl.handle.net/1957/61867

► Zebrafish (Danio rerio) are one of the most commonly used animal models in biomedical research. Zebrafish resource facilities, like the Zebrafish International Resource Center (ZIRC)… (more)

▼ Zebrafish (Danio rerio) are one of the most commonly used animal models in biomedical research. Zebrafish resource facilities, like the Zebrafish International Resource Center (ZIRC) in Eugene, Oregon, are the main providers and keepers of numerous zebrafish wild-type, mutant, and transgenic lines. Although ZIRC maintains live zebrafish at various life stages, sperm cryopreservation allows them to maintain the vast array of zebrafish lines that they receive from outside facilities. Hence, there is a concern about the potential of vertical transmission of pathogens capable of surviving the freezing and thawing process. My first study was to determine whether zebrafish pathogens are capable of surviving the sperm cryopreservation process used by ZIRC (i.e., the ZIRC method). I assessed the survival of two strains of Mycobacterium chelonae (H1E1 and H1E2), one strain of Mycobacterium marinum (OSU 214), one strain of Edwardsiella ictaluri, Pseudocapillaria tomentosa eggs and Pseudoloma neurophilia spores, which are all pathogens of concern in zebrafish research facilities. These pathogens were also frozen and thawed without cryopreservant, and the pathogens were frozen at either -80 °C or - 20 °C with only a small amount of Phosphate Buffered Saline (PBS). Each bacterial species survived both freezing and thawing methods, however the samples subjected to the ZIRC method had the higher percentages of bacterial survival compared to the freezing without cryopreservant samples. The mycobacteria had higher survival rates compared to Gram-negative E. ictaluri in both freezing methods. E. ictaluri exhibited a 1-2 log decrease in concentration following the freezing without cryopreservant. For the P. tomentosa eggs, survival was based on larvation. Eggs were examined at Day 0 (immediately after collecting or thawing) and at Day 7 (a week after being collected or thawed). No larvation was observed on Day 7 with eggs processed by the ZIRC method or simple freezing (-80 °C in 1X PBS and no cryopreservant). In contrast, the positive controls, kept at 28 °C, showed 80-93% larvation at Day 7. Most of the eggs observed in either freezing method were unlarvated and intact, however some, exhibited signs of internal deformation of the egg contents. In 2014, our lab conducted a similar cryopreservation study on the ZIRC cryopreservation method in place at that time. In that study P. neurophilia spores were tested for their ability to survive cryopreservation. I repeated this study in 2017 using the 2017 ZIRC cryopreservation protocol. In both experiments, two fluorescent stains, SYTOX and Fungi-Fluor, and presence of a spore vacuole were used to determine spore viability. SYTOX green is a fluorescent nucleic acid stain, and cells are scored as dead when the dye enters the cells and results in green fluorescence. P. neurophilia spores also contain a long, coiled, polar filament or tube that is thought to aid in infecting hosts cells when extruded. Spores are scored as alive if they are stained with Fungi-Fluor, exposed to ultra violet… Advisors/Committee Members: Kent, Michael L. (advisor), Halsey, Kimberly H. (committee member).

Subjects/Keywords: Zebrafish

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APA (6^th Edition):

Norris, L. J. (2017). Controlling Important Pathogens in Zebrafish (Danio rerio): Assessing Cryopreservation Survival of Bacteria and Parasites and Clinical Sensitivity of Mycobacterial qPCR Assays. (Masters Thesis). Oregon State University. Retrieved from http://hdl.handle.net/1957/61867

Chicago Manual of Style (16^th Edition):

Norris, Lauren J. “Controlling Important Pathogens in Zebrafish (Danio rerio): Assessing Cryopreservation Survival of Bacteria and Parasites and Clinical Sensitivity of Mycobacterial qPCR Assays.” 2017. Masters Thesis, Oregon State University. Accessed July 19, 2019. http://hdl.handle.net/1957/61867.

MLA Handbook (7^th Edition):

Norris, Lauren J. “Controlling Important Pathogens in Zebrafish (Danio rerio): Assessing Cryopreservation Survival of Bacteria and Parasites and Clinical Sensitivity of Mycobacterial qPCR Assays.” 2017. Web. 19 Jul 2019.

Vancouver:

Norris LJ. Controlling Important Pathogens in Zebrafish (Danio rerio): Assessing Cryopreservation Survival of Bacteria and Parasites and Clinical Sensitivity of Mycobacterial qPCR Assays. [Internet] [Masters thesis]. Oregon State University; 2017. [cited 2019 Jul 19]. Available from: http://hdl.handle.net/1957/61867.

Council of Science Editors:

Norris LJ. Controlling Important Pathogens in Zebrafish (Danio rerio): Assessing Cryopreservation Survival of Bacteria and Parasites and Clinical Sensitivity of Mycobacterial qPCR Assays. [Masters Thesis]. Oregon State University; 2017. Available from: http://hdl.handle.net/1957/61867

3. Thorn, Robert Joseph. Development, Disease, and Regeneration: Using Zebrafish to Model Neurological Perturbations.

Degree: Department of Molecular Biology, Cell Biology and Biochemistry, 2018, Brown University

URL: https://repository.library.brown.edu/studio/item/bdr:792820/

► As medical technologies advance, effective vertebrate models of human disease are vital to determine safety and efficacy of treatments. My research has further developed zebrafish… (more)

▼ As medical technologies advance, effective vertebrate models of human disease are vital to determine safety and efficacy of treatments. My research has further developed zebrafish as a model to investigate vertebrate neurological development and disease. Zebrafish are well suited for use as a model of vertebrate disease. A mixed population of male and female fish can produce hundreds of embryos on a daily basis. The embryos are transparent, develop externally, and can be imaged live by light microscopy throughout development. They are accessible to genetic manipulation and have many genes that are homologous to human disease genes. Zebrafish larvae display robust stereotyped behaviors that can be assayed to detect subtle brain defects. In my thesis work, I used zebrafish to model developmental sensitivities to immunosuppressant drugs that may be prescribed during human fetal development. My research indicated that these drugs have a negative effect on brain and behavioral development. I’ve shown that zebrafish are also useful in testing neurodevelopmental toxicity of small molecules. Additionally, I have used morpholinos in zebrafish to knock down calcineurin, whose decreased signaling has been implicated in Down syndrome disease phenotypes. My research displayed developmental brain defects and behavioral defects in the calcineurin morphants, potentially due to increased apoptosis early in development. This model can also be utilized to test pharmaceuticals that may treat Down syndrome phenotypes. Finally, I used zebrafish as a high-throughput model for the loss and recovery of vertebrate vision. My work showed that zebrafish behavior can be used as a method to detect loss and recovery of larval zebrafish vision in multi-well plates. This model will be useful in testing compounds to treat human visual diseases, especially those that may be used in conjunction with promising stem cell therapies. Overall my research has made strides in using zebrafish as a vertebrate model of neurodevelopmental aberrations and potential therapeutic screening of pharmaceutical compounds. Advisors/Committee Members: Hart, Anne (Reader), Wessel, Gary (Reader), Creton, Robbert (Advisor), Mowry, Kimberly (Reader), DeLong, Alison (Reader), Aluru, Neel (Reader).

Subjects/Keywords: Zebrafish

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APA (6^th Edition):

Thorn, R. J. (2018). Development, Disease, and Regeneration: Using Zebrafish to Model Neurological Perturbations. (Thesis). Brown University. Retrieved from https://repository.library.brown.edu/studio/item/bdr:792820/

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Thorn, Robert Joseph. “Development, Disease, and Regeneration: Using Zebrafish to Model Neurological Perturbations.” 2018. Thesis, Brown University. Accessed July 19, 2019. https://repository.library.brown.edu/studio/item/bdr:792820/.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Thorn, Robert Joseph. “Development, Disease, and Regeneration: Using Zebrafish to Model Neurological Perturbations.” 2018. Web. 19 Jul 2019.

Vancouver:

Thorn RJ. Development, Disease, and Regeneration: Using Zebrafish to Model Neurological Perturbations. [Internet] [Thesis]. Brown University; 2018. [cited 2019 Jul 19]. Available from: https://repository.library.brown.edu/studio/item/bdr:792820/.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Thorn RJ. Development, Disease, and Regeneration: Using Zebrafish to Model Neurological Perturbations. [Thesis]. Brown University; 2018. Available from: https://repository.library.brown.edu/studio/item/bdr:792820/

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

4. Ojo, Oladele Temitope Adedayo. Screen for a Novel Pharmaceutical that Stimulates Photoreceptor Regeneration in Zebrafish Utilizing a Behavioral Assay.

Degree: Department of Molecular Biology, Cell Biology and Biochemistry, 2017, Brown University

URL: https://repository.library.brown.edu/studio/item/bdr:733461/

► Zebrafish have demonstrated a remarkable ability to regenerate following damage to their visual system. However, little is known about the underlying mechanisms for this regeneration,… (more)

Subjects/Keywords: Zebrafish

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Ojo, O. T. A. (2017). Screen for a Novel Pharmaceutical that Stimulates Photoreceptor Regeneration in Zebrafish Utilizing a Behavioral Assay. (Thesis). Brown University. Retrieved from https://repository.library.brown.edu/studio/item/bdr:733461/

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Ojo, Oladele Temitope Adedayo. “Screen for a Novel Pharmaceutical that Stimulates Photoreceptor Regeneration in Zebrafish Utilizing a Behavioral Assay.” 2017. Thesis, Brown University. Accessed July 19, 2019. https://repository.library.brown.edu/studio/item/bdr:733461/.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Ojo, Oladele Temitope Adedayo. “Screen for a Novel Pharmaceutical that Stimulates Photoreceptor Regeneration in Zebrafish Utilizing a Behavioral Assay.” 2017. Web. 19 Jul 2019.

Vancouver:

Ojo OTA. Screen for a Novel Pharmaceutical that Stimulates Photoreceptor Regeneration in Zebrafish Utilizing a Behavioral Assay. [Internet] [Thesis]. Brown University; 2017. [cited 2019 Jul 19]. Available from: https://repository.library.brown.edu/studio/item/bdr:733461/.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Ojo OTA. Screen for a Novel Pharmaceutical that Stimulates Photoreceptor Regeneration in Zebrafish Utilizing a Behavioral Assay. [Thesis]. Brown University; 2017. Available from: https://repository.library.brown.edu/studio/item/bdr:733461/

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Manchester

5. Haud, Noemie Magali Renee. A forward genetic screen to identify modifiers of chemotherapy using zebrafish- Study of rnaset2 deficiency in zebrafish.

Degree: 2010, University of Manchester

URL: http://www.manchester.ac.uk/escholar/uk-ac-man-scw:90343

► Chemotherapy frequently fails to cure cancer patients due to toxicity or resistance to treatment. Variability in toxicity and resistance is influenced by polymorphisms and mutations… (more)

▼ Chemotherapy frequently fails to cure cancer patients due to toxicity or resistance to treatment. Variability in toxicity and resistance is influenced by polymorphisms and mutations in the individual's genome (Pharmacogenetics). Zebrafish has extensive evolutionary conservation with human, its genetics is a powerful gene discovery tool, and it has been described as a suitable model in cancer research. To study chemotherapy resistance, we used ENU-mutagenised zebrafish in a forward genetic screen to identify genes that modify responses to cancer chemotherapy. Zebrafish larvae were challenged with two chemotherapeutic drugs and stained with acridine orange (AO) to detect apoptosis and reveal hypo- or hyper-responders to chemotherapy. A mutation, conferring an increased uptake of AO, was identified by genetic mapping as a premature stop codon truncating the ribonuclease T2 (rnaset2) gene. Human RNASET2 encodes a putative lysosomal RNase. Lysosomal storage disorders, due to deficiencies in lysosomal hydrolases and resultant accumulation of macromolecules within lysosomes, are collectively among the commonest genetic diseases. RNASET2 deficiency in man results in a static encephalopathy arising in infancy and characterized by multifocal bilateral white matter lesions, subcortical cysts and focal enlargement of the anterior inferior horn. This doctoral thesis demonstrates that rnaset2 deficient zebrafish embryos suffer from a lysosomal storage disorder accumulating undigested ribosomal RNA (rRNA) in enlarged lysosomes within neurons of the brain. Moreover, high-field intensity μMRI revealed white matter lesions in the brain of adult rnase2 mutant animals. Thus, this zebrafish mutant can be used as a model to study the abnormalities observed in RNASET2 deficient individuals. This model suggests that the leukoencephalopathy results from a lysosomal storage disorder and provides a preclinical model for further elaborating disease mechanisms and evaluating candidate therapeutics.RNASET2 has also been advanced as a candidate tumour suppressor in several solid tumours. Recombinant rnaset2 protein has been tested in the clinic as an anti-cancer cytotoxic agent, with anti-angiogenic properties. By combining the rnaset2 mutant presented here with a transgenic melanoma model developed in the laboratory, the tumour suppressor and angiogenic role for rnaset2 was refuted. Advisors/Committee Members: Hurlstone, Adam.

Subjects/Keywords: lysosomes; zebrafish

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Haud, N. M. R. (2010). A forward genetic screen to identify modifiers of chemotherapy using zebrafish- Study of rnaset2 deficiency in zebrafish. (Doctoral Dissertation). University of Manchester. Retrieved from http://www.manchester.ac.uk/escholar/uk-ac-man-scw:90343

Chicago Manual of Style (16^th Edition):

Haud, Noemie Magali Renee. “A forward genetic screen to identify modifiers of chemotherapy using zebrafish- Study of rnaset2 deficiency in zebrafish.” 2010. Doctoral Dissertation, University of Manchester. Accessed July 19, 2019. http://www.manchester.ac.uk/escholar/uk-ac-man-scw:90343.

MLA Handbook (7^th Edition):

Haud, Noemie Magali Renee. “A forward genetic screen to identify modifiers of chemotherapy using zebrafish- Study of rnaset2 deficiency in zebrafish.” 2010. Web. 19 Jul 2019.

Vancouver:

Haud NMR. A forward genetic screen to identify modifiers of chemotherapy using zebrafish- Study of rnaset2 deficiency in zebrafish. [Internet] [Doctoral dissertation]. University of Manchester; 2010. [cited 2019 Jul 19]. Available from: http://www.manchester.ac.uk/escholar/uk-ac-man-scw:90343.

Council of Science Editors:

Haud NMR. A forward genetic screen to identify modifiers of chemotherapy using zebrafish- Study of rnaset2 deficiency in zebrafish. [Doctoral Dissertation]. University of Manchester; 2010. Available from: http://www.manchester.ac.uk/escholar/uk-ac-man-scw:90343

University of Adelaide

6. Lim, Anne Hwee Ling. Analysis of the subcellular localization of proteins implicated in Alzheimer’s disease.

Degree: 2015, University of Adelaide

URL: http://hdl.handle.net/2440/103503

► Alzheimer’s disease (AD) is a neurodegenerative disorder involving neuropathological changes including the presence of amyloid plaques in the brain. Amyloid plaques are comprised mainly of… (more)

▼ Alzheimer’s disease (AD) is a neurodegenerative disorder involving neuropathological changes including the presence of amyloid plaques in the brain. Amyloid plaques are comprised mainly of the Aβ peptide, formed by the cleavage of the AMYLOID BETA A4 PRECURSOR PROTEIN (APP) by the enzyme complex γ-secretase. The catalytic component of the γ-secretase complex is PRESENILIN (PSEN1 or PSEN2). Mutations in the human PSEN1 gene have been identified in AD. Some mutations have been found to cause frame shifts leading to premature stop codons that produce transcripts encoding truncated PSEN1 protein. The subcellular location of the PSENs has been controversial, with studies detecting these proteins in multiple areas in the cell. However recent research has shown that the PSENs are enriched in the mitochondria associated membranes (MAM). The zebrafish is a small freshwater fish that is a useful animal model for the study of human diseases. Zebrafish have genes that are orthologous to human genes that play a role in AD, including those that encode the components of the γ-secretase complex. However, the zebrafish orthologue of the NICASTRIN gene has not been studied in detail. In Chapter II, we characterize in zebrafish the orthologue of the human NICASTRIN (NCSTN) gene that encodes a protein component of the γ-secretase. We demonstrate the spatial and temporal expression level of zebrafish ncstn in embryos and various adult tissues. This work provides the basic knowledge required to further the understanding of the role of Ncstn in the γ-secretase complex using the zebrafish animal model. Several truncations of the human PSEN1 and zebrafish Psen1 proteins appear to have differential effects on Notch signalling and Appa cleavage. The basis for these differences is still unclear. Chapter III examines the subcellular localization of fusions of truncations of zebrafish Psen1 protein with green fluorescent protein (GFP) using a simple protocol to obtain single cells from zebrafish embryos for microscopy analysis. We show that the different truncations analysed appear to have similar distributions, suggesting that the differential effects on γ-secretase substrates are likely not due to different subcellular localisations of these truncated forms of Psen1. This chapter contributes a new method of viewing a single layer of zebrafish cells with confocal microscopy. The mitochondria-associated membranes (MAM) are a subcompartment of the endoplasmic reticulum (ER) which interacts with mitochondria. The MAM is highly enriched in PSEN1 and PSEN2. Mitochondrion-ER appositions are increased in Psen1⁻ʹ⁻/Psen2⁻ʹ⁻ mouse embryonic fibroblasts (MEFs) and also in familial AD (FAD) and sporadic AD (SAD) patient cells. These results, which were performed using mammalian cells, have not been studied in the zebrafish animal model. Therefore, Chapter IV of this thesis examines the effect of inhibiting zebrafish psen1 and psen2 activity on mitochondrion-ER appositions in 24 hpf embryos. The findings in this study differ from those done in mammalian… Advisors/Committee Members: Lardelli, Michael Trent (advisor), Kelly, Joan Maree (advisor), School of Biological Sciences (school).

Subjects/Keywords: Alzheimer’s; zebrafish

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Lim, A. H. L. (2015). Analysis of the subcellular localization of proteins implicated in Alzheimer’s disease. (Thesis). University of Adelaide. Retrieved from http://hdl.handle.net/2440/103503

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Lim, Anne Hwee Ling. “Analysis of the subcellular localization of proteins implicated in Alzheimer’s disease.” 2015. Thesis, University of Adelaide. Accessed July 19, 2019. http://hdl.handle.net/2440/103503.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Lim, Anne Hwee Ling. “Analysis of the subcellular localization of proteins implicated in Alzheimer’s disease.” 2015. Web. 19 Jul 2019.

Vancouver:

Lim AHL. Analysis of the subcellular localization of proteins implicated in Alzheimer’s disease. [Internet] [Thesis]. University of Adelaide; 2015. [cited 2019 Jul 19]. Available from: http://hdl.handle.net/2440/103503.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Lim AHL. Analysis of the subcellular localization of proteins implicated in Alzheimer’s disease. [Thesis]. University of Adelaide; 2015. Available from: http://hdl.handle.net/2440/103503

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Waikato

7. Ahmadi, Ramtin. Eating behaviour regulation in Zebrafish (Danio rerio): a comparative analysis .

Degree: 2014, University of Waikato

URL: http://hdl.handle.net/10289/8791

► The structure, function, and tissue specific expression profiles of neuropeptides involved in regulating eating behaviour are well researched in mammals, the corresponding literature on fish… (more)

▼ The structure, function, and tissue specific expression profiles of neuropeptides involved in regulating eating behaviour are well researched in mammals, the corresponding literature on fish homologs however is scarce. The work presented here endeavoured to characterise the full coding sequences of peptide homologs in zebrafish as well as the expression profiles of said peptides under different energy states to elucidate their function in fish. Zebrafish were chosen as a model to represent fish species as they are amenable to molecular study. Conducting a literature and TBLASTn search, genes were identified in zebrafish that are known to be involved in affecting appetite and satiety in other vertebrates. Comparison of the translated sequences of these genes against fish and other vertebrates provided confidence in the identity of AGRP, GHRL, POMCb, CART1, CART3, and CART4 and all genes showed homology against mammalian transcripts through structural and syntenic comparison. RACE synthesis of the full coding sequence of these genes was attempted from isolated brain mRNA but only resulted in the production of a partial 3’ sequence for CART1 and CART4 which showed high identity against the predicted nucleotide but not the translated sequence. To observe the functional properties of a suite of genes, a qPCR analysis of the expression profiles of AGRP, GHRL, POMCa, POMCb, CART1, CART2, CART3, CART4, OXT, NPY, and AVPwas undertaken in 24 hour fasted zebrafish. The results were comparable to both other fish and mammals as AGRP was orexigenically upregulated in fasted zebrafish, while POMCb and OXT were anorexigenically downregulated. This is the first time that an expression study has been conducted across all of these genes together in a single species and, while the results are preliminary, they outline that the simultaneous analysis of the many genes thought to be involved in eating behaviour is a viable approach to discovering the functional roles of the range of genes, their interaction with each other, and how that relates to eating behaviour and provides some confidence in use of zebrafish as a model to highlight this. Advisors/Committee Members: Bird, Steve (advisor), Olszewski, Pawel K (advisor).

Subjects/Keywords: Zebrafish; feeding

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Ahmadi, R. (2014). Eating behaviour regulation in Zebrafish (Danio rerio): a comparative analysis . (Masters Thesis). University of Waikato. Retrieved from http://hdl.handle.net/10289/8791

Chicago Manual of Style (16^th Edition):

Ahmadi, Ramtin. “Eating behaviour regulation in Zebrafish (Danio rerio): a comparative analysis .” 2014. Masters Thesis, University of Waikato. Accessed July 19, 2019. http://hdl.handle.net/10289/8791.

MLA Handbook (7^th Edition):

Ahmadi, Ramtin. “Eating behaviour regulation in Zebrafish (Danio rerio): a comparative analysis .” 2014. Web. 19 Jul 2019.

Vancouver:

Ahmadi R. Eating behaviour regulation in Zebrafish (Danio rerio): a comparative analysis . [Internet] [Masters thesis]. University of Waikato; 2014. [cited 2019 Jul 19]. Available from: http://hdl.handle.net/10289/8791.

Council of Science Editors:

Ahmadi R. Eating behaviour regulation in Zebrafish (Danio rerio): a comparative analysis . [Masters Thesis]. University of Waikato; 2014. Available from: http://hdl.handle.net/10289/8791

University of Ottawa

8. Sheppard, Derek. Joint Formation in the Caudal Fin Rays of Danio rerio (Zebrafish) During Fin Regeneration .

Degree: 2017, University of Ottawa

URL: http://hdl.handle.net/10393/37008

► Danio rerio, more commonly called zebrafish, possess the ability to regenerate many of their organs and appendages including complete regeneration of the caudal fin back… (more)

▼ Danio rerio, more commonly called zebrafish, possess the ability to regenerate many of their organs and appendages including complete regeneration of the caudal fin back to its original size. The caudal fin consists of 16-18 fin rays (lepidotrichia) separated by interray tissue. These fin rays consist of bone segments that are partitioned by joints. Each bone segment consists of two oval shaped hemirays that form a tube-like structure that houses a multitude of tissues. During regeneration, the fin ray elongates through the distal addition of bone segments separated by newly formed joints. This is all regulated by a highly proliferative group of cells called the blastema located in the most distal portion of the regenerating fin ray. Our lab has been able to distinguish different stages of joint maturation during the regenerative process which has led to an interest in the effects of joint cell ablation during regeneration. A transgenic line of zebrafish that contained lnta11 regulatory elements was found to give expression of a reporter in the joints of adult zebrafish. We used this regulatory element to drive expression of nitroreductase (NTR) in joint cells. In the presence of NTR, metronidazole (MTZ) induces ablation through initiation of the caspase dependent apoptosis cascade. Previous joint cell photoblation results in our lab indicated that joints may not be able to regenerate, we wanted to determine the results of complete ablation of joint cells during regeneration using the NTR/MTZ inducible targeted ablation technique. Surprisingly treatment with MTZ did not completely eliminate the joints during regeneration. However, there was a reduction in segment length and overall fin length of fish treated with MTZ compared to controls. Furthermore, as the concentration of MTZ is increased there is a proportional reduction in segment length of the fin regenerate. Although we failed to eliminate joints, the phenotype observed provided support for our proposed model of joint formation during regeneration. The creation of transgenic fish lines are underway to allow the separate ectopic expression of two transcription factors; sp7 (a known marker of osteoblast commitment) and hoxa13a (a hypothesized joint commitment factor) during regeneration.

Subjects/Keywords: Zebrafish; Regeneration

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APA (6^th Edition):

Sheppard, D. (2017). Joint Formation in the Caudal Fin Rays of Danio rerio (Zebrafish) During Fin Regeneration . (Thesis). University of Ottawa. Retrieved from http://hdl.handle.net/10393/37008

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Sheppard, Derek. “Joint Formation in the Caudal Fin Rays of Danio rerio (Zebrafish) During Fin Regeneration .” 2017. Thesis, University of Ottawa. Accessed July 19, 2019. http://hdl.handle.net/10393/37008.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Sheppard, Derek. “Joint Formation in the Caudal Fin Rays of Danio rerio (Zebrafish) During Fin Regeneration .” 2017. Web. 19 Jul 2019.

Vancouver:

Sheppard D. Joint Formation in the Caudal Fin Rays of Danio rerio (Zebrafish) During Fin Regeneration . [Internet] [Thesis]. University of Ottawa; 2017. [cited 2019 Jul 19]. Available from: http://hdl.handle.net/10393/37008.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Sheppard D. Joint Formation in the Caudal Fin Rays of Danio rerio (Zebrafish) During Fin Regeneration . [Thesis]. University of Ottawa; 2017. Available from: http://hdl.handle.net/10393/37008

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Manchester

9. Oltrabella, Francesca. INVESTIGATION OF OCRL1 AND ITS INTERACTION PARTNERS IN ZEBRAFISH.

Degree: 2014, University of Manchester

URL: http://www.manchester.ac.uk/escholar/uk-ac-man-scw:240811

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Oculocerebrorenal syndrome of Lowe is a rare X-linked disorder caused bymutation of the inositol 5-phosphatase OCRL1. Lowe Syndrome manifests as renaltubular dysfunction, neurological and ocular… (more)

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Oculocerebrorenal syndrome of Lowe is a rare X-linked disorder caused bymutation of the inositol 5-phosphatase OCRL1. Lowe Syndrome manifests as renaltubular dysfunction, neurological and ocular defects. OCRL1 uses its catalyticdomain to hydrolyze two phosphoinositide species, PI(4,5)P2 and PI3,4,5)P3. It isinvolved in regulation of membrane trafficking, actin dynamics, cytokinesis andciliogenesis. OCRL1 interacts with IPIP27A and B, which have been shown to bekey players in endocytic trafficking in mammalian cells, specifically in the recyclingof proteins from early and recycling endosomes to both the plasma membrane andtrans-Golgi network. It has been proposed that defective endocytic trafficking maybe responsible for the renal tubulopathy seen in Lowe Syndrome patients,characterized by low molecular weight proteinuria and aminoaciduria, but thishypothesis has yet to be tested.Using zebrafish as a model for Lowe syndrome, we show that depletion ofOcrl1 can indeed cause defects in endocytosis in the renal tubule. This coincideswith a reduction in levels of the multi-ligand receptor megalin, reduced abundanceof the endocytic apparatus and increased numbers of enlarged lysosomes in thekidney tubular cells. We also show that knocking-down Pip5K in the Ocrl1 mutantsto rebalance PI(4,5)P2 levels can rescue the endocytic defect. This indicates thattight control of PI(4,5)P2 level is essential for efficient endocytic trafficking in vivo.Importantly, this finding suggests that Pip5K may be a valuable therapeutic targetfor patients with Lowe Syndrome.To further characterize the molecular mechanisms by which OCRL1 promotesendocytosis, we have focused on the recently identified Ocrl1 interaction partnersIPIP27A and B, which are known to function in endocytosis and receptor recycling.Here we report identification and characterization of the zebrafish Ipip27s, includinganalysis of conservation and expression profiles. To assess Ipip27s function invivo, KO zebrafish lines were generated using TALENs. This was successful forIpip27A, but so far not for Ipip27B. Functional analysis using the Ipip27A KO lineand KD with morpholinos revealed that both Ipip27s contribute to neuraldevelopment and may participate in ciliogenesis. Moreover, preliminary analysisindicates an important role for Ipip27A within the endocytic pathway in the kidneytubule, where its loss phenocopies many aspects of the Ocrl1 mutant phenotype.

CD - MOVIE 1 AND MOVIE 2

Advisors/Committee Members: HURLSTONE, ADAM AFL, Hurlstone, Adam, Lowe, Martin.

Subjects/Keywords: OCRL1; ZEBRAFISH

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Oltrabella, F. (2014). INVESTIGATION OF OCRL1 AND ITS INTERACTION PARTNERS IN ZEBRAFISH. (Doctoral Dissertation). University of Manchester. Retrieved from http://www.manchester.ac.uk/escholar/uk-ac-man-scw:240811

Chicago Manual of Style (16^th Edition):

Oltrabella, Francesca. “INVESTIGATION OF OCRL1 AND ITS INTERACTION PARTNERS IN ZEBRAFISH.” 2014. Doctoral Dissertation, University of Manchester. Accessed July 19, 2019. http://www.manchester.ac.uk/escholar/uk-ac-man-scw:240811.

MLA Handbook (7^th Edition):

Oltrabella, Francesca. “INVESTIGATION OF OCRL1 AND ITS INTERACTION PARTNERS IN ZEBRAFISH.” 2014. Web. 19 Jul 2019.

Vancouver:

Oltrabella F. INVESTIGATION OF OCRL1 AND ITS INTERACTION PARTNERS IN ZEBRAFISH. [Internet] [Doctoral dissertation]. University of Manchester; 2014. [cited 2019 Jul 19]. Available from: http://www.manchester.ac.uk/escholar/uk-ac-man-scw:240811.

Council of Science Editors:

Oltrabella F. INVESTIGATION OF OCRL1 AND ITS INTERACTION PARTNERS IN ZEBRAFISH. [Doctoral Dissertation]. University of Manchester; 2014. Available from: http://www.manchester.ac.uk/escholar/uk-ac-man-scw:240811

Vanderbilt University

10. Li, Nan. MiRNA function during early vertebrate development.

Degree: PhD, Biological Sciences, 2010, Vanderbilt University

URL: http://etd.library.vanderbilt.edu//available/etd-07092010-001651/ ;

► microRNAs (miRNAs) are short non-coding RNAs that down-regulate gene expression by pairing with sequences in the 3âUTR of target mRNAs. They play critical roles in… (more)

Subjects/Keywords: zebrafish; microRNA

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Li, N. (2010). MiRNA function during early vertebrate development. (Doctoral Dissertation). Vanderbilt University. Retrieved from http://etd.library.vanderbilt.edu//available/etd-07092010-001651/ ;

Chicago Manual of Style (16^th Edition):

Li, Nan. “MiRNA function during early vertebrate development.” 2010. Doctoral Dissertation, Vanderbilt University. Accessed July 19, 2019. http://etd.library.vanderbilt.edu//available/etd-07092010-001651/ ;.

MLA Handbook (7^th Edition):

Li, Nan. “MiRNA function during early vertebrate development.” 2010. Web. 19 Jul 2019.

Vancouver:

Li N. MiRNA function during early vertebrate development. [Internet] [Doctoral dissertation]. Vanderbilt University; 2010. [cited 2019 Jul 19]. Available from: http://etd.library.vanderbilt.edu//available/etd-07092010-001651/ ;.

Council of Science Editors:

Li N. MiRNA function during early vertebrate development. [Doctoral Dissertation]. Vanderbilt University; 2010. Available from: http://etd.library.vanderbilt.edu//available/etd-07092010-001651/ ;

University of Alberta

11. Brewster, Daniel L. Voltage gated potassium currents in the Mauthner and MiD2cm cells of larval zebrafish.

Degree: MS, Department of Biological Sciences, 2012, University of Alberta

URL: https://era.library.ualberta.ca/files/wh246s285

► The escape response in zebrafish is mediated in part by the Mauthner cell and its two homologues, MiD2cm and MiD3cm. In adult fish, the Mauthner… (more)

Subjects/Keywords: Mauthner; Potassium; Zebrafish

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APA (6^th Edition):

Brewster, D. L. (2012). Voltage gated potassium currents in the Mauthner and MiD2cm cells of larval zebrafish. (Masters Thesis). University of Alberta. Retrieved from https://era.library.ualberta.ca/files/wh246s285

Chicago Manual of Style (16^th Edition):

Brewster, Daniel L. “Voltage gated potassium currents in the Mauthner and MiD2cm cells of larval zebrafish.” 2012. Masters Thesis, University of Alberta. Accessed July 19, 2019. https://era.library.ualberta.ca/files/wh246s285.

MLA Handbook (7^th Edition):

Brewster, Daniel L. “Voltage gated potassium currents in the Mauthner and MiD2cm cells of larval zebrafish.” 2012. Web. 19 Jul 2019.

Vancouver:

Brewster DL. Voltage gated potassium currents in the Mauthner and MiD2cm cells of larval zebrafish. [Internet] [Masters thesis]. University of Alberta; 2012. [cited 2019 Jul 19]. Available from: https://era.library.ualberta.ca/files/wh246s285.

Council of Science Editors:

Brewster DL. Voltage gated potassium currents in the Mauthner and MiD2cm cells of larval zebrafish. [Masters Thesis]. University of Alberta; 2012. Available from: https://era.library.ualberta.ca/files/wh246s285

University of Manchester

12. Haud, Noemie Magali Renee. A forward genetic screen to identify modifiers of chemotherapy using zebrafish : study of rnaset2 deficiency in zebrafish.

Degree: PhD, 2010, University of Manchester

URL: https://www.research.manchester.ac.uk/portal/en/theses/a-forward-genetic-screen-to-identify-modifiers-of-chemotherapy-using-zebrafish-study-of-rnaset2-deficiency-in-zebrafish(234f73f0-6d31-4832-a4b6-315687376b90).html ; http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.529238

► Chemotherapy frequently fails to cure cancer patients due to toxicity or resistance to treatment. Variability in toxicity and resistance is influenced by polymorphisms and mutations… (more)

▼ Chemotherapy frequently fails to cure cancer patients due to toxicity or resistance to treatment. Variability in toxicity and resistance is influenced by polymorphisms and mutations in the individual's genome (Pharmacogenetics). Zebrafish has extensive evolutionary conservation with human, its genetics is a powerful gene discovery tool, and it has been described as a suitable model in cancer research. To study chemotherapy resistance, we used ENU-mutagenised zebrafish in a forward genetic screen to identify genes that modify responses to cancer chemotherapy. Zebrafish larvae were challenged with two chemotherapeutic drugs and stained with acridine orange (AO) to detect apoptosis and reveal hypo- or hyper-responders to chemotherapy. A mutation, conferring an increased uptake of AO, was identified by genetic mapping as a premature stop codon truncating the ribonuclease T2 (rnaset2) gene. Human RNASET2 encodes a putative lysosomal RNase. Lysosomal storage disorders, due to deficiencies in lysosomal hydrolases and resultant accumulation of macromolecules within lysosomes, are collectively among the commonest genetic diseases. RNASET2 deficiency in man results in a static encephalopathy arising in infancy and characterized by multifocal bilateral white matter lesions, subcortical cysts and focal enlargement of the anterior inferior horn. This doctoral thesis demonstrates that rnaset2 deficient zebrafish embryos suffer from a lysosomal storage disorder accumulating undigested ribosomal RNA (rRNA) in enlarged lysosomes within neurons of the brain. Moreover, high-field intensity μMRI revealed white matter lesions in the brain of adult rnase2 mutant animals. Thus, this zebrafish mutant can be used as a model to study the abnormalities observed in RNASET2 deficient individuals. This model suggests that the leukoencephalopathy results from a lysosomal storage disorder and provides a preclinical model for further elaborating disease mechanisms and evaluating candidate therapeutics.RNASET2 has also been advanced as a candidate tumour suppressor in several solid tumours. Recombinant rnaset2 protein has been tested in the clinic as an anti-cancer cytotoxic agent, with anti-angiogenic properties. By combining the rnaset2 mutant presented here with a transgenic melanoma model developed in the laboratory, the tumour suppressor and angiogenic role for rnaset2 was refuted.

Subjects/Keywords: 616.042; lysosomes; zebrafish

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Haud, N. M. R. (2010). A forward genetic screen to identify modifiers of chemotherapy using zebrafish : study of rnaset2 deficiency in zebrafish. (Doctoral Dissertation). University of Manchester. Retrieved from https://www.research.manchester.ac.uk/portal/en/theses/a-forward-genetic-screen-to-identify-modifiers-of-chemotherapy-using-zebrafish-study-of-rnaset2-deficiency-in-zebrafish(234f73f0-6d31-4832-a4b6-315687376b90).html ; http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.529238

Chicago Manual of Style (16^th Edition):

Haud, Noemie Magali Renee. “A forward genetic screen to identify modifiers of chemotherapy using zebrafish : study of rnaset2 deficiency in zebrafish.” 2010. Doctoral Dissertation, University of Manchester. Accessed July 19, 2019. https://www.research.manchester.ac.uk/portal/en/theses/a-forward-genetic-screen-to-identify-modifiers-of-chemotherapy-using-zebrafish-study-of-rnaset2-deficiency-in-zebrafish(234f73f0-6d31-4832-a4b6-315687376b90).html ; http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.529238.

MLA Handbook (7^th Edition):

Haud, Noemie Magali Renee. “A forward genetic screen to identify modifiers of chemotherapy using zebrafish : study of rnaset2 deficiency in zebrafish.” 2010. Web. 19 Jul 2019.

Vancouver:

Haud NMR. A forward genetic screen to identify modifiers of chemotherapy using zebrafish : study of rnaset2 deficiency in zebrafish. [Internet] [Doctoral dissertation]. University of Manchester; 2010. [cited 2019 Jul 19]. Available from: https://www.research.manchester.ac.uk/portal/en/theses/a-forward-genetic-screen-to-identify-modifiers-of-chemotherapy-using-zebrafish-study-of-rnaset2-deficiency-in-zebrafish(234f73f0-6d31-4832-a4b6-315687376b90).html ; http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.529238.

Council of Science Editors:

Haud NMR. A forward genetic screen to identify modifiers of chemotherapy using zebrafish : study of rnaset2 deficiency in zebrafish. [Doctoral Dissertation]. University of Manchester; 2010. Available from: https://www.research.manchester.ac.uk/portal/en/theses/a-forward-genetic-screen-to-identify-modifiers-of-chemotherapy-using-zebrafish-study-of-rnaset2-deficiency-in-zebrafish(234f73f0-6d31-4832-a4b6-315687376b90).html ; http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.529238

University of Saskatchewan

13. Kallarakavumkal Thomas, Jith. Effects of dietary and in ovo selenomethionine exposure in zebrafish.

Degree: 2014, University of Saskatchewan

URL: http://hdl.handle.net/10388/ETD-2014-09-1744

► Selenium (Se) is an essential trace element to most living organisms, however when compared to other ingested essential trace elements Se has the lowest margin… (more)

▼ Selenium (Se) is an essential trace element to most living organisms, however when compared to other ingested essential trace elements Se has the lowest margin of safety between essential and toxic concentrations. Oviparous vertebrates, especially fishes, are highly susceptible to dietary Se toxicity. Greater incidences of deformities and/or mortalities have been observed in F1 generation larval fishes whose parents were exposed to excess dietary Se in the form of selenomethionine (SeMet), however little information is available on effects of chronic dietary SeMet exposure to adult fish and persistent effects of in ovo SeMet exposure to F1 generation fish. This thesis investigated effects of chronic dietary exposure of excess Se in the form of SeMet on swimming performance (Ucrit), oxygen consumption (MO2), stored energy (triglycerides and glycogen), and the physiological stress response (cortisol production) in adult zebrafish (Danio rerio), as well as immediate (incidence of deformities and mortality) and persistent (e.g. changes in Ucrit, MO2, bioenergetics, the physiological stress response and reproduction) effects of in ovo exposure to SeMet in F1 generation zebrafish. In addition, the study investigated potential underlying mechanisms of SeMet-induced developmental toxicities in early life stages of zebrafish using embryo microinjection. Two separate dietary SeMet exposure studies in adult zebrafish and two in ovo SeMet maternal transfer studies in F1 generation zebrafish were conducted. The first dietary or in ovo exposure study explored effects of excess SeMet exposure on adult zebrafish or the entire life cycle of F1 generation zebrafish. The second study investigated mechanisms of observed SeMet-induced effects on adult or F1 generation zebrafish. In the first feeding study, a significant reduction in Ucrit and greater accumulation of stored energy were observed in the excess dietary SeMet exposed groups when compared to the Se-sufficient dietary control group. The second feeding study showed a greater metabolic rate, and impaired aerobic energy metabolism and triglyceride homeostasis in adult fish fed excess dietary SeMet, which was associated with a reduction in swimming performance and accumulation of triglycerides. Embryos collected from adult zebrafish in both dietary SeMet exposure studies were used to investigate effects of in ovo SeMet exposure on the entire life cycle of F1 generation fish. The first study showed a greater incidence of mortality, an increasing trend for deformities in F1 generation larval zebrafish, and reduced Ucrit in F1 generation adult fish exposed to excess SeMet via in ovo maternal transfer. However, concentrations of stored energy, cortisol and reproduction were unaltered. The second study found that impaired aerobic performance might have been responsible for the reduction in Ucrit of F1 generation adult zebrafish exposed to excess SeMet. Since there is a high variability in Se deposition among eggs via natural maternal transfer, SeMet embryo microinjection… Advisors/Committee Members: Janz, David M., Blakley, Barry, Krone, Pat, Niyogi, Som, Drew, Murray.

Subjects/Keywords: Selenomethionine Zebrafish Toxicity

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Kallarakavumkal Thomas, J. (2014). Effects of dietary and in ovo selenomethionine exposure in zebrafish. (Thesis). University of Saskatchewan. Retrieved from http://hdl.handle.net/10388/ETD-2014-09-1744

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Kallarakavumkal Thomas, Jith. “Effects of dietary and in ovo selenomethionine exposure in zebrafish.” 2014. Thesis, University of Saskatchewan. Accessed July 19, 2019. http://hdl.handle.net/10388/ETD-2014-09-1744.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Kallarakavumkal Thomas, Jith. “Effects of dietary and in ovo selenomethionine exposure in zebrafish.” 2014. Web. 19 Jul 2019.

Vancouver:

Kallarakavumkal Thomas J. Effects of dietary and in ovo selenomethionine exposure in zebrafish. [Internet] [Thesis]. University of Saskatchewan; 2014. [cited 2019 Jul 19]. Available from: http://hdl.handle.net/10388/ETD-2014-09-1744.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Kallarakavumkal Thomas J. Effects of dietary and in ovo selenomethionine exposure in zebrafish. [Thesis]. University of Saskatchewan; 2014. Available from: http://hdl.handle.net/10388/ETD-2014-09-1744

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Manchester

14. Oltrabella, Francesca. Investigation of OCRL1 and its interaction partners in zebrafish.

Degree: PhD, 2014, University of Manchester

URL: https://www.research.manchester.ac.uk/portal/en/theses/investigation-of-ocrl1-and-its-interaction-partners-in-zebrafish(77a97cd8-f030-4c4e-93cc-fe4e2ea44ee6).html ; https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.764293

► Oculocerebrorenal syndrome of Lowe is a rare X-linked disorder caused by mutation of the inositol 5-phosphatase OCRL1. Lowe Syndrome manifests as renal tubular dysfunction, neurological… (more)

▼ Oculocerebrorenal syndrome of Lowe is a rare X-linked disorder caused by mutation of the inositol 5-phosphatase OCRL1. Lowe Syndrome manifests as renal tubular dysfunction, neurological and ocular defects. OCRL1 uses its catalytic domain to hydrolyze two phosphoinositide species, PI(4,5)P2 and PI3,4,5)P3. It is involved in regulation of membrane trafficking, actin dynamics, cytokinesis and ciliogenesis. OCRL1 interacts with IPIP27A and B, which have been shown to be key players in endocytic trafficking in mammalian cells, specifically in the recycling of proteins from early and recycling endosomes to both the plasma membrane and trans-Golgi network. It has been proposed that defective endocytic trafficking may be responsible for the renal tubulopathy seen in Lowe Syndrome patients, characterized by low molecular weight proteinuria and aminoaciduria, but this hypothesis has yet to be tested. Using zebrafish as a model for Lowe syndrome, we show that depletion of OCRL1 can indeed cause defects in endocytosis in the renal tubule. This coincides with a reduction in levels of the multi-ligand receptor megalin, reduced abundance of the endocytic apparatus and increased numbers of enlarged lysosomes in the kidney tubular cells. We also show that knocking-down Pip5K in the OCRL1 mutants to rebalance PI(4,5)P2 levels can rescue the endocytic defect. This indicates that tight control of PI(4,5)P2 level is essential for efficient endocytic trafficking in vivo. Importantly, this finding suggests that Pip5K may be a valuable therapeutic target for patients with Lowe Syndrome. To further characterize the molecular mechanisms by which OCRL1 promotes endocytosis, we have focused on the recently identified OCRL1 interaction partners IPIP27A and B, which are known to function in endocytosis and receptor recycling. Here we report identification and characterization of the zebrafish Ipip27s, including analysis of conservation and expression profiles. To assess Ipip27s function in vivo, KO zebrafish lines were generated using TALENs. This was successful for Ipip27A, but so far not for Ipip27B. Functional analysis using the Ipip27A KO line and KD with morpholinos revealed that both Ipip27s contribute to neural development and may participate in ciliogenesis. Moreover, preliminary analysis indicates an important role for Ipip27A within the endocytic pathway in the kidney tubule, where its loss phenocopies many aspects of the OCRL1 mutant phenotype.

Subjects/Keywords: 570; ZEBRAFISH; OCRL1

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Oltrabella, F. (2014). Investigation of OCRL1 and its interaction partners in zebrafish. (Doctoral Dissertation). University of Manchester. Retrieved from https://www.research.manchester.ac.uk/portal/en/theses/investigation-of-ocrl1-and-its-interaction-partners-in-zebrafish(77a97cd8-f030-4c4e-93cc-fe4e2ea44ee6).html ; https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.764293

Chicago Manual of Style (16^th Edition):

Oltrabella, Francesca. “Investigation of OCRL1 and its interaction partners in zebrafish.” 2014. Doctoral Dissertation, University of Manchester. Accessed July 19, 2019. https://www.research.manchester.ac.uk/portal/en/theses/investigation-of-ocrl1-and-its-interaction-partners-in-zebrafish(77a97cd8-f030-4c4e-93cc-fe4e2ea44ee6).html ; https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.764293.

MLA Handbook (7^th Edition):

Oltrabella, Francesca. “Investigation of OCRL1 and its interaction partners in zebrafish.” 2014. Web. 19 Jul 2019.

Vancouver:

Oltrabella F. Investigation of OCRL1 and its interaction partners in zebrafish. [Internet] [Doctoral dissertation]. University of Manchester; 2014. [cited 2019 Jul 19]. Available from: https://www.research.manchester.ac.uk/portal/en/theses/investigation-of-ocrl1-and-its-interaction-partners-in-zebrafish(77a97cd8-f030-4c4e-93cc-fe4e2ea44ee6).html ; https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.764293.

Council of Science Editors:

Oltrabella F. Investigation of OCRL1 and its interaction partners in zebrafish. [Doctoral Dissertation]. University of Manchester; 2014. Available from: https://www.research.manchester.ac.uk/portal/en/theses/investigation-of-ocrl1-and-its-interaction-partners-in-zebrafish(77a97cd8-f030-4c4e-93cc-fe4e2ea44ee6).html ; https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.764293

Mississippi State University

15. Varner, Casey Janine. IN VIVO COMPARISON OF EDWARDSIELLA ICTALURI SURVIVAL IN KIDNEYS OF VACCINATED AND NAÏVE RAG1^-/- ZEBRAFISH.

Degree: MS, Veterinary Medicine, College of, 2010, Mississippi State University

URL: http://sun.library.msstate.edu/ETD-db/theses/available/etd-04282010-141111/ ;

► This study used rag1^-/- mutant zebrafish, which lack functional T and B lymphocytes, to investigate whether innate immune cells from vaccinated mutant zebrafish demonstrate… (more)

Subjects/Keywords: zebrafish; Edwardsiella ictaluri

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APA (6^th Edition):

Varner, C. J. (2010). IN VIVO COMPARISON OF EDWARDSIELLA ICTALURI SURVIVAL IN KIDNEYS OF VACCINATED AND NAÏVE RAG1^-/- ZEBRAFISH. (Masters Thesis). Mississippi State University. Retrieved from http://sun.library.msstate.edu/ETD-db/theses/available/etd-04282010-141111/ ;

Chicago Manual of Style (16^th Edition):

Varner, Casey Janine. “IN VIVO COMPARISON OF EDWARDSIELLA ICTALURI SURVIVAL IN KIDNEYS OF VACCINATED AND NAÏVE RAG1^-/- ZEBRAFISH.” 2010. Masters Thesis, Mississippi State University. Accessed July 19, 2019. http://sun.library.msstate.edu/ETD-db/theses/available/etd-04282010-141111/ ;.

MLA Handbook (7^th Edition):

Varner, Casey Janine. “IN VIVO COMPARISON OF EDWARDSIELLA ICTALURI SURVIVAL IN KIDNEYS OF VACCINATED AND NAÏVE RAG1^-/- ZEBRAFISH.” 2010. Web. 19 Jul 2019.

Vancouver:

Varner CJ. IN VIVO COMPARISON OF EDWARDSIELLA ICTALURI SURVIVAL IN KIDNEYS OF VACCINATED AND NAÏVE RAG1^-/- ZEBRAFISH. [Internet] [Masters thesis]. Mississippi State University; 2010. [cited 2019 Jul 19]. Available from: http://sun.library.msstate.edu/ETD-db/theses/available/etd-04282010-141111/ ;.

Council of Science Editors:

Varner CJ. IN VIVO COMPARISON OF EDWARDSIELLA ICTALURI SURVIVAL IN KIDNEYS OF VACCINATED AND NAÏVE RAG1^-/- ZEBRAFISH. [Masters Thesis]. Mississippi State University; 2010. Available from: http://sun.library.msstate.edu/ETD-db/theses/available/etd-04282010-141111/ ;

University of Ottawa

16. Boratynska, Susan. Biological Confinement of Zebrafish Using RNAi .

Degree: 2015, University of Ottawa

URL: http://hdl.handle.net/10393/32775

► The increasing demand for fish in the food industry has resulted in the extensive overfishing of wild fisheries. In efforts to alleviate the demand from… (more)

▼ The increasing demand for fish in the food industry has resulted in the extensive overfishing of wild fisheries. In efforts to alleviate the demand from the food industry, genetically modified (GM) fish were developed possessing traits such as larger mass, faster growth, and increased resistance to disease. However, the greater fitness advantage of GM fish presents potential risks for wild type populations in the event of release or escape from a confined fish facility. In addition to physical barriers, it is critical to develop a genetic mechanism in order to ensure that the spread of GM transgene to the natural populations does not occur. RNA interference (RNAi) is an endogenous mechanism used to regulate gene expression by destroying targeted mRNA molecules. Manipulation of this biological process has been successfully utilized to knockdown specific genes through the introduction of synthetic transgenes in organisms such as C. elegans, M. musculus, and D. melanogaster. Although the use of RNAi as a biological tool is still relatively new in zebrafish, recent work has explored elements of this mechanism allowing for greater knockdown efficiencies. The deadend (dnd) gene is required for primordial germ cell (PGC) development and survival. Previous studies have shown that zebrafish dnd knockouts develop into sterile adults without disrupting somatic development. In efforts to induce sterility in zebrafish, short hairpin RNA (shRNA) constructs targeting dnd were designed to exploit the endogenous RNAi pathway. Upon qualitative analysis in transient and transgenic zebrafish subjected to the synthetic RNAi construct, a reduction in the germ cell population at early stages of development was observed. However, quantification of dnd mRNA in fish from the same time points did not show significant changes in expression levels compared to their wildtype counterparts. Adult fish subjected to the transgene construct produced viable gametes. The use of RNAi as a tool for bioconfinement relies on sterility among all individuals subjected to the shRNA bearing transgene. Based on the results obtained, the verdict is still unclear as to whether shRNA is a viable mechanism for large scale bioconfinement.

Subjects/Keywords: RNAi; dnd; Zebrafish

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Boratynska, S. (2015). Biological Confinement of Zebrafish Using RNAi . (Thesis). University of Ottawa. Retrieved from http://hdl.handle.net/10393/32775

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Boratynska, Susan. “Biological Confinement of Zebrafish Using RNAi .” 2015. Thesis, University of Ottawa. Accessed July 19, 2019. http://hdl.handle.net/10393/32775.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Boratynska, Susan. “Biological Confinement of Zebrafish Using RNAi .” 2015. Web. 19 Jul 2019.

Vancouver:

Boratynska S. Biological Confinement of Zebrafish Using RNAi . [Internet] [Thesis]. University of Ottawa; 2015. [cited 2019 Jul 19]. Available from: http://hdl.handle.net/10393/32775.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Boratynska S. Biological Confinement of Zebrafish Using RNAi . [Thesis]. University of Ottawa; 2015. Available from: http://hdl.handle.net/10393/32775

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Edinburgh

17. Davies, Nicholas Oliver. Function, regeneration and neuroprotection of dopaminergic neurons in the zebrafish.

Degree: PhD, 2016, University of Edinburgh

URL: http://hdl.handle.net/1842/25858

► The zebrafish has an amazing capacity for regeneration which includes regeneration of neurons within the central nervous system (CNS) both during development and into adulthood.… (more)

▼ The zebrafish has an amazing capacity for regeneration which includes regeneration of neurons within the central nervous system (CNS) both during development and into adulthood. This attribute makes the zebrafish a valuable tool in the study of regeneration. In this thesis, the research focussed on the regeneration of a specific type of cell in the CNS, dopaminergic (DA) neurons. The DA system of the zebrafish is believed to be evolutionarily conserved with comparable DA populations found in the brain of mammals. Dissimilar to mammals, however, the zebrafish is capable of regenerating various types of neurons and their axons. Thus, the zebrafish DA system provides an excellent model to study replacement of this specific and important cell type in the adult CNS. We have developed a novel toxin ablation paradigm to specifically ablate select groups of DA neurons in the adult zebrafish diencephalon, leaving other DA populations unaffected. To do this a selective DA toxin, 6-hydroxydopamine, was used. One of the ablated DA diencephalic populations is the only source of dopaminergic spinal innervation in the zebrafish. Their ablation leads to a loss of DA spinal axons following our toxin ablation. The ascending projection of the diencephalic population ablated by the toxin has been suggested as the most likely candidate for a zebrafish equivalent of the mammalian nigro-striatal pathway. The loss of cells is very specific and reproducible, indicating that these cells are particularly vulnerable to the toxin. Quantification of affected populations at various time-points post ablation was carried out to determine the capacity for regeneration of DA neurons in the CNS of zebrafish. This revealed that in some populations neuron numbers returned to those seen in controls. However, in other populations neuron numbers only partially recovered even at late time points. We have shown that this recovery is due to neurogenesis; furthermore, by inducing inflammation after the toxin treatment the recovery of DA cell numbers was accelerated by 50%. Regenerated cells originated from Olig2 positive ependymo-radial glial cells found bordering the diencephalic ventricle. We aimed to investigate the function of this group of ablated neurons through a battery of behavioural tests. These tests revealed deficits in the toxin treated animals’ fine movement, such as is necessary for maintaining shoal cohesion and breeding behaviours, whereas general movement behaviours were not found to be impaired. Zebrafish embryos also present as a great resource in the screening of drugs. Their fast and well characterised early development makes them an ideal tool for investigating previously untested neuroprotectants. A reproducible ablation paradigm similar to that established in the adults was also established in the zebrafish embryo. This was then used as a tool to investigate potential novel neuroprotectants. This screen revealed two new flavonoid compounds which had the ability to induce full protection of the affected dopaminergic cells in the…

Subjects/Keywords: dopamine; regeneration; zebrafish

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APA (6^th Edition):

Davies, N. O. (2016). Function, regeneration and neuroprotection of dopaminergic neurons in the zebrafish. (Doctoral Dissertation). University of Edinburgh. Retrieved from http://hdl.handle.net/1842/25858

Chicago Manual of Style (16^th Edition):

Davies, Nicholas Oliver. “Function, regeneration and neuroprotection of dopaminergic neurons in the zebrafish.” 2016. Doctoral Dissertation, University of Edinburgh. Accessed July 19, 2019. http://hdl.handle.net/1842/25858.

MLA Handbook (7^th Edition):

Davies, Nicholas Oliver. “Function, regeneration and neuroprotection of dopaminergic neurons in the zebrafish.” 2016. Web. 19 Jul 2019.

Vancouver:

Davies NO. Function, regeneration and neuroprotection of dopaminergic neurons in the zebrafish. [Internet] [Doctoral dissertation]. University of Edinburgh; 2016. [cited 2019 Jul 19]. Available from: http://hdl.handle.net/1842/25858.

Council of Science Editors:

Davies NO. Function, regeneration and neuroprotection of dopaminergic neurons in the zebrafish. [Doctoral Dissertation]. University of Edinburgh; 2016. Available from: http://hdl.handle.net/1842/25858

Deakin University

18. Mohd Noor, Suzita. Role of Jak2/Stat5/Cis pathway components in zebrafish development.

Degree: School of Medicine, 2011, Deakin University

URL: http://hdl.handle.net/10536/DRO/DU:30036712

► By utilizing the zebrafish as a research model, the function of specific cell signalling pathway components in development was investigated. This revealed new roles for… (more)

Subjects/Keywords: embryology; cytokines; zebrafish

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APA (6^th Edition):

Mohd Noor, S. (2011). Role of Jak2/Stat5/Cis pathway components in zebrafish development. (Thesis). Deakin University. Retrieved from http://hdl.handle.net/10536/DRO/DU:30036712

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Mohd Noor, Suzita. “Role of Jak2/Stat5/Cis pathway components in zebrafish development.” 2011. Thesis, Deakin University. Accessed July 19, 2019. http://hdl.handle.net/10536/DRO/DU:30036712.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Mohd Noor, Suzita. “Role of Jak2/Stat5/Cis pathway components in zebrafish development.” 2011. Web. 19 Jul 2019.

Vancouver:

Mohd Noor S. Role of Jak2/Stat5/Cis pathway components in zebrafish development. [Internet] [Thesis]. Deakin University; 2011. [cited 2019 Jul 19]. Available from: http://hdl.handle.net/10536/DRO/DU:30036712.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Mohd Noor S. Role of Jak2/Stat5/Cis pathway components in zebrafish development. [Thesis]. Deakin University; 2011. Available from: http://hdl.handle.net/10536/DRO/DU:30036712

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Rice University

19. Rivera Longsworth, Gia Francesca. Expanding the Enzymatic Activity of the Programmable Endonuclease Cas9.

Degree: MA, Natural Sciences, 2018, Rice University

URL: http://hdl.handle.net/1911/105841

► The CRISPR-Cas9 gene editing system has revolutionized our ability to make targeted mutations in a variety of organisms. In zebrafish, we are able to use… (more)

▼ The CRISPR-Cas9 gene editing system has revolutionized our ability to make targeted mutations in a variety of organisms. In zebrafish, we are able to use microinjections of Cas9 protein or mRNA alongside guide RNAs (gRNA) to create targeted insertions and deletions (indels) at a frequency sufficient for recovery of loss of function alleles. Combined with the many practical advantages of working with zebrafish, CRISPR-Cas9 has enabled many labs to study gene function at a lowered cost. However, there are improvements that can be made to facilitate the knock in of sequences into the genome, a process which remains inefficient in zebrafish due to a low frequency of homology directed repair (HDR), a mechanism necessary for knock in. Our aim is to increase the rate of HDR in zebrafish by modifying Cas9 using two approaches. First, we hypothesize that the long latency period of the Cas9-DNA complex taken with the short cell cycle of the early zebrafish embryo is biasing the repair mechanism against HDR, so I have engineered a CL1 degron-tagged Cas9 (DegCas9) to destabilize the Cas9 protein. A second approach to increasing the rate of knock in is to reduce the number of molecules involved in the HDR process by using an RNA template for recombination. We hypothesize that the rate of HDR can be improved if the recombination template remains in proximity to the DSB. To this aim, I have created a reverse-transcriptase Cas9 fusion and modified gRNAs that include the targeting sequence for recombination. Our goal is to create a Cas9 system that efficiently uses an RNA template for recombination. Results indicate that DegCas9 is capable of inducing mutations at a reduced rate, and that the modified gRNAs for the RTCas9 system do not reduce the effectiveness of Cas9 function. Further studies will include analyzing the spectrum of lesions induced by DegCas9 and creating alternative versions of DegCas9 and RTCas9 using different domains. If successful, these Cas9 constructs will expand the toolkit available for genetic engineering in zebrafish. Advisors/Committee Members: Wagner, Daniel (advisor).

Subjects/Keywords: zebrafish; Cas9; CRISPR

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APA (6^th Edition):

Rivera Longsworth, G. F. (2018). Expanding the Enzymatic Activity of the Programmable Endonuclease Cas9. (Masters Thesis). Rice University. Retrieved from http://hdl.handle.net/1911/105841

Chicago Manual of Style (16^th Edition):

Rivera Longsworth, Gia Francesca. “Expanding the Enzymatic Activity of the Programmable Endonuclease Cas9.” 2018. Masters Thesis, Rice University. Accessed July 19, 2019. http://hdl.handle.net/1911/105841.

MLA Handbook (7^th Edition):

Rivera Longsworth, Gia Francesca. “Expanding the Enzymatic Activity of the Programmable Endonuclease Cas9.” 2018. Web. 19 Jul 2019.

Vancouver:

Rivera Longsworth GF. Expanding the Enzymatic Activity of the Programmable Endonuclease Cas9. [Internet] [Masters thesis]. Rice University; 2018. [cited 2019 Jul 19]. Available from: http://hdl.handle.net/1911/105841.

Council of Science Editors:

Rivera Longsworth GF. Expanding the Enzymatic Activity of the Programmable Endonuclease Cas9. [Masters Thesis]. Rice University; 2018. Available from: http://hdl.handle.net/1911/105841

20. Boyle, Cody Adam. Intergenerational Effects Of Embryonic Cocaine Exposure In Zebrafish.

Degree: MS, Biology, 2017, University of North Dakota

URL: https://commons.und.edu/theses/2174

► Cocaine addiction has both genetic and environmentally driven components. Relatively recently several groups have suggested that epigenetic regulation of gene expression might be the… (more)

Subjects/Keywords: Cocaine; Intergenerational; Zebrafish

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APA (6^th Edition):

Boyle, C. A. (2017). Intergenerational Effects Of Embryonic Cocaine Exposure In Zebrafish. (Masters Thesis). University of North Dakota. Retrieved from https://commons.und.edu/theses/2174

Chicago Manual of Style (16^th Edition):

Boyle, Cody Adam. “Intergenerational Effects Of Embryonic Cocaine Exposure In Zebrafish.” 2017. Masters Thesis, University of North Dakota. Accessed July 19, 2019. https://commons.und.edu/theses/2174.

MLA Handbook (7^th Edition):

Boyle, Cody Adam. “Intergenerational Effects Of Embryonic Cocaine Exposure In Zebrafish.” 2017. Web. 19 Jul 2019.

Vancouver:

Boyle CA. Intergenerational Effects Of Embryonic Cocaine Exposure In Zebrafish. [Internet] [Masters thesis]. University of North Dakota; 2017. [cited 2019 Jul 19]. Available from: https://commons.und.edu/theses/2174.

Council of Science Editors:

Boyle CA. Intergenerational Effects Of Embryonic Cocaine Exposure In Zebrafish. [Masters Thesis]. University of North Dakota; 2017. Available from: https://commons.und.edu/theses/2174

University of Arizona

21. Todd, Douglas Wallace. Zebrafish Video Analysis System for High-Throughput Drug Assay .

Degree: 2016, University of Arizona

URL: http://hdl.handle.net/10150/623150

► Zebrafish swimming behavior is used in a new, automated drug assay system as a biomarker to measure drug efficiency to prevent or restore hearing loss.… (more)

Subjects/Keywords: zebrafish; image analysis

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APA (6^th Edition):

Todd, D. W. (2016). Zebrafish Video Analysis System for High-Throughput Drug Assay . (Masters Thesis). University of Arizona. Retrieved from http://hdl.handle.net/10150/623150

Chicago Manual of Style (16^th Edition):

Todd, Douglas Wallace. “Zebrafish Video Analysis System for High-Throughput Drug Assay .” 2016. Masters Thesis, University of Arizona. Accessed July 19, 2019. http://hdl.handle.net/10150/623150.

MLA Handbook (7^th Edition):

Todd, Douglas Wallace. “Zebrafish Video Analysis System for High-Throughput Drug Assay .” 2016. Web. 19 Jul 2019.

Vancouver:

Todd DW. Zebrafish Video Analysis System for High-Throughput Drug Assay . [Internet] [Masters thesis]. University of Arizona; 2016. [cited 2019 Jul 19]. Available from: http://hdl.handle.net/10150/623150.

Council of Science Editors:

Todd DW. Zebrafish Video Analysis System for High-Throughput Drug Assay . [Masters Thesis]. University of Arizona; 2016. Available from: http://hdl.handle.net/10150/623150

University of North Texas

22. Adhikari, Prem R. Proteomic Responses in the Gill of Zebrafish Following Exposure to Ibuprofen and Naproxen.

Degree: 2012, University of North Texas

URL: https://digital.library.unt.edu/ark:/67531/metadc149557/

► Non-steroidal anti-inflammatory drugs (NSAIDs) are among the most abundant environmental pharmaceutical contaminants. In this study, a proteomic analysis was conducted to identify proteins differentially expressed… (more)

Subjects/Keywords: Proteomics; NSAID; zebrafish

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APA (6^th Edition):

Adhikari, P. R. (2012). Proteomic Responses in the Gill of Zebrafish Following Exposure to Ibuprofen and Naproxen. (Thesis). University of North Texas. Retrieved from https://digital.library.unt.edu/ark:/67531/metadc149557/

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Adhikari, Prem R. “Proteomic Responses in the Gill of Zebrafish Following Exposure to Ibuprofen and Naproxen.” 2012. Thesis, University of North Texas. Accessed July 19, 2019. https://digital.library.unt.edu/ark:/67531/metadc149557/.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Adhikari, Prem R. “Proteomic Responses in the Gill of Zebrafish Following Exposure to Ibuprofen and Naproxen.” 2012. Web. 19 Jul 2019.

Vancouver:

Adhikari PR. Proteomic Responses in the Gill of Zebrafish Following Exposure to Ibuprofen and Naproxen. [Internet] [Thesis]. University of North Texas; 2012. [cited 2019 Jul 19]. Available from: https://digital.library.unt.edu/ark:/67531/metadc149557/.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Adhikari PR. Proteomic Responses in the Gill of Zebrafish Following Exposure to Ibuprofen and Naproxen. [Thesis]. University of North Texas; 2012. Available from: https://digital.library.unt.edu/ark:/67531/metadc149557/

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of North Texas

23. Alsrhani, Abdullah Falleh. Novel Role of Trypsin in Zebrafish.

Degree: 2013, University of North Texas

URL: https://digital.library.unt.edu/ark:/67531/metadc271771/

► It has been shown previously in our laboratory that zebrafish produce trypsin from their gills when they are under stress, and this trypsin is involved… (more)

Subjects/Keywords: Trypsin; zebrafish; thrombocyte

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APA (6^th Edition):

Alsrhani, A. F. (2013). Novel Role of Trypsin in Zebrafish. (Thesis). University of North Texas. Retrieved from https://digital.library.unt.edu/ark:/67531/metadc271771/

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Alsrhani, Abdullah Falleh. “Novel Role of Trypsin in Zebrafish.” 2013. Thesis, University of North Texas. Accessed July 19, 2019. https://digital.library.unt.edu/ark:/67531/metadc271771/.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Alsrhani, Abdullah Falleh. “Novel Role of Trypsin in Zebrafish.” 2013. Web. 19 Jul 2019.

Vancouver:

Alsrhani AF. Novel Role of Trypsin in Zebrafish. [Internet] [Thesis]. University of North Texas; 2013. [cited 2019 Jul 19]. Available from: https://digital.library.unt.edu/ark:/67531/metadc271771/.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Alsrhani AF. Novel Role of Trypsin in Zebrafish. [Thesis]. University of North Texas; 2013. Available from: https://digital.library.unt.edu/ark:/67531/metadc271771/

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Universidade Estadual de Campinas

24. Oliveira, Renato Aparecido de, 1986-. Investigação do perfil de miRNAs em cérebro de zebrafish após indução de crises epilépticas recorrentes .

Degree: 2015, Universidade Estadual de Campinas

URL: http://repositorio.unicamp.br/jspui/handle/REPOSIP/312552

► Resumo: As epilepsias formam um conjunto de doenças neurológicas crônicas marcadas por eventos de interrupção recorrente e imprevisível do funcionamento normal do cérebro, denominados crises… (more)

▼ Resumo: As epilepsias formam um conjunto de doenças neurológicas crônicas marcadas por eventos de interrupção recorrente e imprevisível do funcionamento normal do cérebro, denominados crises epilépticas. O status epilepticus (SE) é uma condição grave, considerada como emergência neurológica devido ao seu potencial para causar danos irreversíveis ao cérebro e até mesmo levar à morte. Modelos que visem uma melhor compreensão os mecanismos moleculares que permeiam as crises epilépticas, sejam eles dentro de SE ou não, podem propiciar novas alternativas de tratamento. Nesse estudo foram empregados dois protocolos de indução de crises epilépticas (agudo e SE) no modelo do zebrafish com sete dias de vida pós-fertilização tendo o pentilenotetrazol como agente convulsivante. Nosso objetivo foi investigar o perfil de miRNAs no cérebro desses animais por meio da tecnologia de sequenciamento de nova geração em equipamento HiSeq 2500. Os miRNAs dre-miR-460-3p, dre-miR-725-3p, dre-miR-31, dre-miR-132-5p, dre-miR-459-3p, dre-miR-221-5p e dre-miR-192 se apresentaram diferencialmente expressos (p ajustado < 0,01) nas comparações entre grupos controle, agudo e SE. De modo geral esses miRNAs estão envolvidos em importantes vias de sinalização celular como a diferenciação, o crescimento, sobrevivência e apoptose; Abstract: Epilepsy is the most common form of chronic neurological disease characterized by recurrent seizures. Status epilepticus (SE) is a severe condition, considered as clinical emergency because of its potential to cause irreversible brain damage and even lead to death. Animal models can be used for better understanding of the molecular mechanisms underlying seizures and open perspectives for new therapeutic targets in this disease. In this study, we aimed to established a protocol of SE and analyze the differential expression of miRNAs in zebrafish brain after pentilenetetrazole-induced seizure by using two different protocols of chemical seizure induction (acute and SE). For this purpose, we used the next generation sequencing technology (HiSeq 2500) with subsequent bioinformatics analysis. Differently expressed miRNAs (dre-miR-460-3p, dre-miR-725-3p, dre-miR-31, miR-132-5p-dre, dre-miR-459-3p, dre-miR-221-5p and dre- miR-192; adjusted p-value <0.01) were investigated by in silico analysis, and the gene target identified. In general, target genes are involved in key cell signaling pathways such as differentiation, growth, survival, and apoptosis Advisors/Committee Members: Maurer-Morelli, Claudia Vianna, 1966- (advisor), Morelli, Cláudia Vianna Maurer (advisor), Gonçalves, Edmilson Ricardo (committee member), Torres, Fabio Rossi (committee member).

Subjects/Keywords: MicroRNAs; Zebrafish; Estado epiléptico; Epilepsia

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APA (6^th Edition):

Oliveira, Renato Aparecido de, 1. (2015). Investigação do perfil de miRNAs em cérebro de zebrafish após indução de crises epilépticas recorrentes . (Thesis). Universidade Estadual de Campinas. Retrieved from http://repositorio.unicamp.br/jspui/handle/REPOSIP/312552

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Oliveira, Renato Aparecido de, 1986-. “Investigação do perfil de miRNAs em cérebro de zebrafish após indução de crises epilépticas recorrentes .” 2015. Thesis, Universidade Estadual de Campinas. Accessed July 19, 2019. http://repositorio.unicamp.br/jspui/handle/REPOSIP/312552.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Oliveira, Renato Aparecido de, 1986-. “Investigação do perfil de miRNAs em cérebro de zebrafish após indução de crises epilépticas recorrentes .” 2015. Web. 19 Jul 2019.

Vancouver:

Oliveira, Renato Aparecido de 1. Investigação do perfil de miRNAs em cérebro de zebrafish após indução de crises epilépticas recorrentes . [Internet] [Thesis]. Universidade Estadual de Campinas; 2015. [cited 2019 Jul 19]. Available from: http://repositorio.unicamp.br/jspui/handle/REPOSIP/312552.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Oliveira, Renato Aparecido de 1. Investigação do perfil de miRNAs em cérebro de zebrafish após indução de crises epilépticas recorrentes . [Thesis]. Universidade Estadual de Campinas; 2015. Available from: http://repositorio.unicamp.br/jspui/handle/REPOSIP/312552

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Edinburgh

25. Kuscha, Veronika. Cellular and axonal plasticity in the lesioned spinal cord of adult zebrafish.

Degree: PhD, 2011, University of Edinburgh

URL: http://hdl.handle.net/1842/5912

► Zebrafish, in contrast to mammals, are capable of functional regeneration after complete transection of the spinal cord. In this system I asked: (1) Which spinal… (more)

▼ Zebrafish, in contrast to mammals, are capable of functional regeneration after complete transection of the spinal cord. In this system I asked: (1) Which spinal cell types regenerate in the lesioned spinal cord? (2) To what extent do the dopaminergic and 5-HT systems regenerate and (3) do dopaminergic axons from the brain influence cellular regeneration in the spinal cord? (1) Lost motor neurons are replaced by newly born motor neurons that mature and are integrated into the spinal circuitry after a spinal lesion in adult zebrafish. Using immunohistochemical and transgenic markers in combination with BrdU labeling, we showed that also 5-HT, parvalbuminergic, Pax2+ and Vsx1+ cells are newly born after lesion. Thus, my work shows that diverse cell types are newly generated in the lesioned spinal cord of adult zebrafish. (2) After spinal cord lesion, zebrafish completely recover locomotion within six weeks. Previous work suggested that axonal regeneration is crucial for functional recovery. Here I analyzed changes in the density of 5-HT and dopaminergic axon terminals in the lesioned spinal cord during recovery. Rostral to the lesion site, I observed die-back and sprouting of dopaminergic axons within two weeks post-lesion. Caudal of the lesion, axons are lost indicating Wallerian degeneration. At six weeks post-lesion I tested functional recovery with a behavioral swim test. In recovered fish, a third of the axonal density was restored just caudal of the lesion site, but not at far caudal levels. In contrast, in fish that had non-recovered, only few axons had bridged the lesion site. Thus dopaminergic axon regrowth correlates with functional recovery. Re-transection of the spinal cord in recovered animals abolished re-gained swimming capability, suggesting that behavioral recovery critically depends on axons that crossed the spinal lesion site and not on an intraspinal circuit. 5-HT axon terminals are of both intra- and supraspinal origin. The overall time course of changes in axon terminal density during recovery is similar to that of dopaminergic axon terminals and also correlates with functional recovery. Overall, the organization of the spinal dopaminergic and 5-HT systems, consisting of neuronal somata in the spinal cord and descending axons, differs significantly from their unlesioned organization. I observe sprouting rostral to the lesion site and limited innervation of the caudal spinal cord, as axons do not regrow into the far distal spinal cord. (3) We further hypothesized that signals released by descending axons are involved in cellular regeneration around the lesion site. Dopaminergic axons of supraspinal origin sprout rostral, but are almost completely absent caudal to the lesion site at two weeks post-lesion. Moreover, we observe that expression of the dopamine receptor drd4a is only increased rostral to the lesion site in the ventricular zone of progenitor cells, including olig2 expressing motor neuron progenitor cells. Correlated with these rostro-caudal differences, numbers of regenerating motor…

Subjects/Keywords: 571.1; spinal cord; zebrafish; regeneration

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APA (6^th Edition):

Kuscha, V. (2011). Cellular and axonal plasticity in the lesioned spinal cord of adult zebrafish. (Doctoral Dissertation). University of Edinburgh. Retrieved from http://hdl.handle.net/1842/5912

Chicago Manual of Style (16^th Edition):

Kuscha, Veronika. “Cellular and axonal plasticity in the lesioned spinal cord of adult zebrafish.” 2011. Doctoral Dissertation, University of Edinburgh. Accessed July 19, 2019. http://hdl.handle.net/1842/5912.

MLA Handbook (7^th Edition):

Kuscha, Veronika. “Cellular and axonal plasticity in the lesioned spinal cord of adult zebrafish.” 2011. Web. 19 Jul 2019.

Vancouver:

Kuscha V. Cellular and axonal plasticity in the lesioned spinal cord of adult zebrafish. [Internet] [Doctoral dissertation]. University of Edinburgh; 2011. [cited 2019 Jul 19]. Available from: http://hdl.handle.net/1842/5912.

Council of Science Editors:

Kuscha V. Cellular and axonal plasticity in the lesioned spinal cord of adult zebrafish. [Doctoral Dissertation]. University of Edinburgh; 2011. Available from: http://hdl.handle.net/1842/5912

Universiteit Utrecht

26. Stumpf, Miriam. Interplay of PTEN subcellular localization and catalytic activities in vivo.

Degree: 2016, Universiteit Utrecht

URL: http://dspace.library.uu.nl:8080/handle/1874/334160

► This thesis describes the use of mammalian cells, S. cerevisiae and D. rerio to unravel the complex interplay of PTEN subcellular localization and catalytic activities.… (more)

▼ This thesis describes the use of mammalian cells, S. cerevisiae and D. rerio to unravel the complex interplay of PTEN subcellular localization and catalytic activities. In Chapter 1 we provide a general introduction to the PI3K/Akt(PKB)/PTEN axis, PTEN phosphatase-dependent and –independent functions and regulation of those functions through changes in protein conformation and subcellular localization. Last, we give a short résumé on the role of PTEN in human health and disease. In Chapter 2, we give an overview of developmental and pathological processes that have been successfully studied in zebrafish pten models. Further, we introduce techniques that we and others have developed for modulation of Pten expression in vivo and for the establishment of zebrafish cell lines from tumors. In Chapter 3 we performed a comprehensive mutational and functional analysis of the PTEN N-terminus. To analyze the contribution of this region to PTEN tumor suppressor function in vivo, we studied a panel of tumor-related mutations, employing S.cerevisiae and mammalian cells. We found that most tumor-related N-terminal PTEN mutations that lead to a loss of PIP3-phosphatase activity also showed impaired nuclear localization. This suggests that PIP3 catalytic activity and nuclear localization of PTEN are coordinated by the PTEN N-terminus in an overlapping manner. Our results from soft agar colony formation assays further indicated that both the PTEN PIP3 phosphatase activity and the PTEN capacity to accumulate in the nucleus were important for the full tumor suppression capacity of PTEN. In Chapter 4 we focused on the classical protein import pathway as a possible major nuclear transport mechanism for PTEN and, by immunofluorescence and confocal microscopy performed in mammalian cells, we identified importin alpha3 as a factor involved in PTEN nuclear translocation. Further, we found that the PTEN N-terminal region (residues 1-32) could mediate nuclear accumulation of PTEN through interactions with nuclear transporters. Systematic introduction of point mutations in the importin alpha 3 substrate binding pockets allowed us to further narrow down the region of importin alpha 3 that is important for nuclear accumulation of PTEN to the minor binding pocket. Using the zebrafish as a model organism, in Chapter 5 we unveil a differential requirement of Pten lipid and protein phosphatase activity during embryonic development. To this end, we performed rescue assays. We propose that the role of Pten during angiogenesis mainly consists of suppressing PI3K signaling via its lipid phosphatase activity, whereas the complex process of embryonic development requires lipid and protein phosphatase activity of Pten. In Chapter 6 we characterize the subcellular localization and the functional consquences of the expression of open conformation PTEN. The functional hyperactivity of open conformation PTEN in comparison to PTEN wild type in our model seemed to result predominantly from its enhanced recruitment to the cytoplasmic membrane. In conclusion, we… Advisors/Committee Members: Bakkers, Jeroen, den Hertog, J..

Subjects/Keywords: PTEN; Zebrafish; Cancer; Angiogenesis; Importins

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APA (6^th Edition):

Stumpf, M. (2016). Interplay of PTEN subcellular localization and catalytic activities in vivo. (Doctoral Dissertation). Universiteit Utrecht. Retrieved from http://dspace.library.uu.nl:8080/handle/1874/334160

Chicago Manual of Style (16^th Edition):

Stumpf, Miriam. “Interplay of PTEN subcellular localization and catalytic activities in vivo.” 2016. Doctoral Dissertation, Universiteit Utrecht. Accessed July 19, 2019. http://dspace.library.uu.nl:8080/handle/1874/334160.

MLA Handbook (7^th Edition):

Stumpf, Miriam. “Interplay of PTEN subcellular localization and catalytic activities in vivo.” 2016. Web. 19 Jul 2019.

Vancouver:

Stumpf M. Interplay of PTEN subcellular localization and catalytic activities in vivo. [Internet] [Doctoral dissertation]. Universiteit Utrecht; 2016. [cited 2019 Jul 19]. Available from: http://dspace.library.uu.nl:8080/handle/1874/334160.

Council of Science Editors:

Stumpf M. Interplay of PTEN subcellular localization and catalytic activities in vivo. [Doctoral Dissertation]. Universiteit Utrecht; 2016. Available from: http://dspace.library.uu.nl:8080/handle/1874/334160

NSYSU

27. Song , Yi-Chun. Coral-derived natural marine compound GB9 inhibits vascular development in zebrafish.

Degree: Master, Biological Sciences, 2017, NSYSU

URL: http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0716117-115842

► Vascular development is important for vertebrates, and genetic control vascular patterning has been intensively characterized. In addition, many studies have been show environmental hormones and… (more)

▼ Vascular development is important for vertebrates, and genetic control vascular patterning has been intensively characterized. In addition, many studies have been show environmental hormones and chemical compounds affect vascular growth. Natural marine compound GB9 was isolated from the marine soft coral Capnella imbricate, the study show GB9 had anti-neuroinflammatory and anti-nociceptive effects. However, the effect of GB9 on blood vessels is still unknown. In this study, we use transgene zebrafish as an animal model to understand the effect of vascular development in GB9 treated embryo. The results showed that GB9 treated embryos impair ISV (intersegmental vessel) growth, and CVP (caudal vein plexus) sprouting, which leads to pericardial edema and circulation defects. TUNEL assay and AO staining showed that vascular defects were not caused by apoptosis, but likely due to the impairment of cell proliferation and/or migration. We further showed GB9 treatment reduce cell proliferation marker p-HH3 and protein level, by counting p-HH3 immunostaining cell and Western blotting. In addition, we quantitated the migration distance of ISV between 24hpf and 28hpf and perform wound healing assay in endothelial cells EA.hy926. We show that GB9 treatment inhibits cell migration. Next, we examined the molecular mechanism of vascular defects by in situ hybridization, qPCR and Western blot, the results showed GB9 treatment decreases the expression of arteriovenous markers, ephrinb2, flt4, mrc1, flk and stabilinï¼the protein levels related to VEGF, BMP signal pathways. Those results suggest that GB9 interfere vascular signaling regulation in zebrafish. Besides, we examine whether GB9 treatment cause vascular defect due to the increase of oxidative stress? We co-treated low-dose GB9 and H2O2, observed that defects in CVP formation was more severe than single treatment. Moreover, antioxidants Acetyl-cysteine (NAC) can rescue vascular defects in GB9 treated embryos. These data suggested that GB9 exposure causes vascular defects mediated by an increase in oxidative. Together our data show that GB9 treatment affect vascular development mediated by the interference of VEGF and BMP signals by the enhance of oxidative stress. Advisors/Committee Members: Tzu-Fun Fu (chair), Zhi-Hong Wen (chair), Yung-Jen Chuang (chair), Chang-Yi Wu (committee member), ming-hong Tai (chair).

Subjects/Keywords: zebrafish; marine compound; angiogenesis; GB9

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Song , Y. (2017). Coral-derived natural marine compound GB9 inhibits vascular development in zebrafish. (Thesis). NSYSU. Retrieved from http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0716117-115842

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Song , Yi-Chun. “Coral-derived natural marine compound GB9 inhibits vascular development in zebrafish.” 2017. Thesis, NSYSU. Accessed July 19, 2019. http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0716117-115842.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Song , Yi-Chun. “Coral-derived natural marine compound GB9 inhibits vascular development in zebrafish.” 2017. Web. 19 Jul 2019.

Vancouver:

Song Y. Coral-derived natural marine compound GB9 inhibits vascular development in zebrafish. [Internet] [Thesis]. NSYSU; 2017. [cited 2019 Jul 19]. Available from: http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0716117-115842.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Song Y. Coral-derived natural marine compound GB9 inhibits vascular development in zebrafish. [Thesis]. NSYSU; 2017. Available from: http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0716117-115842

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

NSYSU

28. Tu, Chia-Wen. Effects of exercise hormone irisin in glucocorticoid-induced osteoporosis.

Degree: Master, Biological Sciences, 2017, NSYSU

URL: http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0723117-220830

► Regular exercise stimulated the expression of the Fibronectin type III domain-containing protein 5 (FNDC5) in skeletal muscle. In post-translation modification process, FNDC5 cleavage to a… (more)

Subjects/Keywords: GIOP; IrisinR75E; Irisin; Zebrafish

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Tu, C. (2017). Effects of exercise hormone irisin in glucocorticoid-induced osteoporosis. (Thesis). NSYSU. Retrieved from http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0723117-220830

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Tu, Chia-Wen. “Effects of exercise hormone irisin in glucocorticoid-induced osteoporosis.” 2017. Thesis, NSYSU. Accessed July 19, 2019. http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0723117-220830.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Tu, Chia-Wen. “Effects of exercise hormone irisin in glucocorticoid-induced osteoporosis.” 2017. Web. 19 Jul 2019.

Vancouver:

Tu C. Effects of exercise hormone irisin in glucocorticoid-induced osteoporosis. [Internet] [Thesis]. NSYSU; 2017. [cited 2019 Jul 19]. Available from: http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0723117-220830.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Tu C. Effects of exercise hormone irisin in glucocorticoid-induced osteoporosis. [Thesis]. NSYSU; 2017. Available from: http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0723117-220830

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Edinburgh

29. Capper, Amy. MITF in melanoma progression, pathology and survival in vivo.

Degree: PhD, 2014, University of Edinburgh

URL: http://hdl.handle.net/1842/10035

► MITF is the master melanocyte transcription factor and has a complex role in melanoma. Both gain- and loss-of function mutations in MITF have been identified… (more)

Subjects/Keywords: 616.99; MITF; melanoma; zebrafish

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Capper, A. (2014). MITF in melanoma progression, pathology and survival in vivo. (Doctoral Dissertation). University of Edinburgh. Retrieved from http://hdl.handle.net/1842/10035

Chicago Manual of Style (16^th Edition):

Capper, Amy. “MITF in melanoma progression, pathology and survival in vivo.” 2014. Doctoral Dissertation, University of Edinburgh. Accessed July 19, 2019. http://hdl.handle.net/1842/10035.

MLA Handbook (7^th Edition):

Capper, Amy. “MITF in melanoma progression, pathology and survival in vivo.” 2014. Web. 19 Jul 2019.

Vancouver:

Capper A. MITF in melanoma progression, pathology and survival in vivo. [Internet] [Doctoral dissertation]. University of Edinburgh; 2014. [cited 2019 Jul 19]. Available from: http://hdl.handle.net/1842/10035.

Council of Science Editors:

Capper A. MITF in melanoma progression, pathology and survival in vivo. [Doctoral Dissertation]. University of Edinburgh; 2014. Available from: http://hdl.handle.net/1842/10035

University of Vermont

30. Light, Sarah. The role of PLXNA1 in visual system development of Danio rerio.

Degree: Neuroscience, 2015, University of Vermont

URL: https://scholarworks.uvm.edu/hcoltheses/220

► Plexin (PLXN) receptors, and their ligands, semaphorins (SEMAs), traditionally mediate axon growth in the visual system by facilitating actin cytoskeletal rearrangements and initiating growth… (more)

Subjects/Keywords: zebrafish; eye; development; plexin; semaphorin

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Light, S. (2015). The role of PLXNA1 in visual system development of Danio rerio. (Thesis). University of Vermont. Retrieved from https://scholarworks.uvm.edu/hcoltheses/220

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Light, Sarah. “The role of PLXNA1 in visual system development of Danio rerio.” 2015. Thesis, University of Vermont. Accessed July 19, 2019. https://scholarworks.uvm.edu/hcoltheses/220.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Light, Sarah. “The role of PLXNA1 in visual system development of Danio rerio.” 2015. Web. 19 Jul 2019.

Vancouver:

Light S. The role of PLXNA1 in visual system development of Danio rerio. [Internet] [Thesis]. University of Vermont; 2015. [cited 2019 Jul 19]. Available from: https://scholarworks.uvm.edu/hcoltheses/220.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Light S. The role of PLXNA1 in visual system development of Danio rerio. [Thesis]. University of Vermont; 2015. Available from: https://scholarworks.uvm.edu/hcoltheses/220

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

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Open Access Theses and Dissertations