subject:(zebrafish)

1. Thorn, Robert Joseph. Development, Disease, and Regeneration: Using Zebrafish to Model Neurological Perturbations.

Degree: Department of Molecular Biology, Cell Biology and Biochemistry, 2018, Brown University

URL: https://repository.library.brown.edu/studio/item/bdr:792820/

► As medical technologies advance, effective vertebrate models of human disease are vital to determine safety and efficacy of treatments. My research has further developed zebrafish… (more)

▼ As medical technologies advance, effective vertebrate models of human disease are vital to determine safety and efficacy of treatments. My research has further developed zebrafish as a model to investigate vertebrate neurological development and disease. Zebrafish are well suited for use as a model of vertebrate disease. A mixed population of male and female fish can produce hundreds of embryos on a daily basis. The embryos are transparent, develop externally, and can be imaged live by light microscopy throughout development. They are accessible to genetic manipulation and have many genes that are homologous to human disease genes. Zebrafish larvae display robust stereotyped behaviors that can be assayed to detect subtle brain defects. In my thesis work, I used zebrafish to model developmental sensitivities to immunosuppressant drugs that may be prescribed during human fetal development. My research indicated that these drugs have a negative effect on brain and behavioral development. I’ve shown that zebrafish are also useful in testing neurodevelopmental toxicity of small molecules. Additionally, I have used morpholinos in zebrafish to knock down calcineurin, whose decreased signaling has been implicated in Down syndrome disease phenotypes. My research displayed developmental brain defects and behavioral defects in the calcineurin morphants, potentially due to increased apoptosis early in development. This model can also be utilized to test pharmaceuticals that may treat Down syndrome phenotypes. Finally, I used zebrafish as a high-throughput model for the loss and recovery of vertebrate vision. My work showed that zebrafish behavior can be used as a method to detect loss and recovery of larval zebrafish vision in multi-well plates. This model will be useful in testing compounds to treat human visual diseases, especially those that may be used in conjunction with promising stem cell therapies. Overall my research has made strides in using zebrafish as a vertebrate model of neurodevelopmental aberrations and potential therapeutic screening of pharmaceutical compounds. Advisors/Committee Members: Hart, Anne (Reader), Wessel, Gary (Reader), Creton, Robbert (Advisor), Mowry, Kimberly (Reader), DeLong, Alison (Reader), Aluru, Neel (Reader).

Subjects/Keywords: Zebrafish

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APA (6^th Edition):

Thorn, R. J. (2018). Development, Disease, and Regeneration: Using Zebrafish to Model Neurological Perturbations. (Thesis). Brown University. Retrieved from https://repository.library.brown.edu/studio/item/bdr:792820/

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Thorn, Robert Joseph. “Development, Disease, and Regeneration: Using Zebrafish to Model Neurological Perturbations.” 2018. Thesis, Brown University. Accessed March 01, 2021. https://repository.library.brown.edu/studio/item/bdr:792820/.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Thorn, Robert Joseph. “Development, Disease, and Regeneration: Using Zebrafish to Model Neurological Perturbations.” 2018. Web. 01 Mar 2021.

Vancouver:

Thorn RJ. Development, Disease, and Regeneration: Using Zebrafish to Model Neurological Perturbations. [Internet] [Thesis]. Brown University; 2018. [cited 2021 Mar 01]. Available from: https://repository.library.brown.edu/studio/item/bdr:792820/.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Thorn RJ. Development, Disease, and Regeneration: Using Zebrafish to Model Neurological Perturbations. [Thesis]. Brown University; 2018. Available from: https://repository.library.brown.edu/studio/item/bdr:792820/

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

2. Ojo, Oladele Temitope Adedayo. Screen for a Novel Pharmaceutical that Stimulates Photoreceptor Regeneration in Zebrafish Utilizing a Behavioral Assay.

Degree: Department of Molecular Biology, Cell Biology and Biochemistry, 2017, Brown University

URL: https://repository.library.brown.edu/studio/item/bdr:733461/

► Zebrafish have demonstrated a remarkable ability to regenerate following damage to their visual system. However, little is known about the underlying mechanisms for this regeneration,… (more)

Subjects/Keywords: Zebrafish

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APA (6^th Edition):

Ojo, O. T. A. (2017). Screen for a Novel Pharmaceutical that Stimulates Photoreceptor Regeneration in Zebrafish Utilizing a Behavioral Assay. (Thesis). Brown University. Retrieved from https://repository.library.brown.edu/studio/item/bdr:733461/

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Ojo, Oladele Temitope Adedayo. “Screen for a Novel Pharmaceutical that Stimulates Photoreceptor Regeneration in Zebrafish Utilizing a Behavioral Assay.” 2017. Thesis, Brown University. Accessed March 01, 2021. https://repository.library.brown.edu/studio/item/bdr:733461/.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Ojo, Oladele Temitope Adedayo. “Screen for a Novel Pharmaceutical that Stimulates Photoreceptor Regeneration in Zebrafish Utilizing a Behavioral Assay.” 2017. Web. 01 Mar 2021.

Vancouver:

Ojo OTA. Screen for a Novel Pharmaceutical that Stimulates Photoreceptor Regeneration in Zebrafish Utilizing a Behavioral Assay. [Internet] [Thesis]. Brown University; 2017. [cited 2021 Mar 01]. Available from: https://repository.library.brown.edu/studio/item/bdr:733461/.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Ojo OTA. Screen for a Novel Pharmaceutical that Stimulates Photoreceptor Regeneration in Zebrafish Utilizing a Behavioral Assay. [Thesis]. Brown University; 2017. Available from: https://repository.library.brown.edu/studio/item/bdr:733461/

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Oregon State University

3. Norris, Lauren J. Controlling Important Pathogens in Zebrafish (Danio rerio): Assessing Cryopreservation Survival of Bacteria and Parasites and Clinical Sensitivity of Mycobacterial qPCR Assays.

Degree: MS, 2017, Oregon State University

URL: http://hdl.handle.net/1957/61867

► Zebrafish (Danio rerio) are one of the most commonly used animal models in biomedical research. Zebrafish resource facilities, like the Zebrafish International Resource Center (ZIRC)… (more)

▼ Zebrafish (Danio rerio) are one of the most commonly used animal models in biomedical research. Zebrafish resource facilities, like the Zebrafish International Resource Center (ZIRC) in Eugene, Oregon, are the main providers and keepers of numerous zebrafish wild-type, mutant, and transgenic lines. Although ZIRC maintains live zebrafish at various life stages, sperm cryopreservation allows them to maintain the vast array of zebrafish lines that they receive from outside facilities. Hence, there is a concern about the potential of vertical transmission of pathogens capable of surviving the freezing and thawing process. My first study was to determine whether zebrafish pathogens are capable of surviving the sperm cryopreservation process used by ZIRC (i.e., the ZIRC method). I assessed the survival of two strains of Mycobacterium chelonae (H1E1 and H1E2), one strain of Mycobacterium marinum (OSU 214), one strain of Edwardsiella ictaluri, Pseudocapillaria tomentosa eggs and Pseudoloma neurophilia spores, which are all pathogens of concern in zebrafish research facilities. These pathogens were also frozen and thawed without cryopreservant, and the pathogens were frozen at either -80 °C or - 20 °C with only a small amount of Phosphate Buffered Saline (PBS). Each bacterial species survived both freezing and thawing methods, however the samples subjected to the ZIRC method had the higher percentages of bacterial survival compared to the freezing without cryopreservant samples. The mycobacteria had higher survival rates compared to Gram-negative E. ictaluri in both freezing methods. E. ictaluri exhibited a 1-2 log decrease in concentration following the freezing without cryopreservant. For the P. tomentosa eggs, survival was based on larvation. Eggs were examined at Day 0 (immediately after collecting or thawing) and at Day 7 (a week after being collected or thawed). No larvation was observed on Day 7 with eggs processed by the ZIRC method or simple freezing (-80 °C in 1X PBS and no cryopreservant). In contrast, the positive controls, kept at 28 °C, showed 80-93% larvation at Day 7. Most of the eggs observed in either freezing method were unlarvated and intact, however some, exhibited signs of internal deformation of the egg contents. In 2014, our lab conducted a similar cryopreservation study on the ZIRC cryopreservation method in place at that time. In that study P. neurophilia spores were tested for their ability to survive cryopreservation. I repeated this study in 2017 using the 2017 ZIRC cryopreservation protocol. In both experiments, two fluorescent stains, SYTOX and Fungi-Fluor, and presence of a spore vacuole were used to determine spore viability. SYTOX green is a fluorescent nucleic acid stain, and cells are scored as dead when the dye enters the cells and results in green fluorescence. P. neurophilia spores also contain a long, coiled, polar filament or tube that is thought to aid in infecting hosts cells when extruded. Spores are scored as alive if they are stained with Fungi-Fluor, exposed to ultra violet… Advisors/Committee Members: Kent, Michael L. (advisor), Halsey, Kimberly H. (committee member).

Subjects/Keywords: Zebrafish

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APA (6^th Edition):

Norris, L. J. (2017). Controlling Important Pathogens in Zebrafish (Danio rerio): Assessing Cryopreservation Survival of Bacteria and Parasites and Clinical Sensitivity of Mycobacterial qPCR Assays. (Masters Thesis). Oregon State University. Retrieved from http://hdl.handle.net/1957/61867

Chicago Manual of Style (16^th Edition):

Norris, Lauren J. “Controlling Important Pathogens in Zebrafish (Danio rerio): Assessing Cryopreservation Survival of Bacteria and Parasites and Clinical Sensitivity of Mycobacterial qPCR Assays.” 2017. Masters Thesis, Oregon State University. Accessed March 01, 2021. http://hdl.handle.net/1957/61867.

MLA Handbook (7^th Edition):

Norris, Lauren J. “Controlling Important Pathogens in Zebrafish (Danio rerio): Assessing Cryopreservation Survival of Bacteria and Parasites and Clinical Sensitivity of Mycobacterial qPCR Assays.” 2017. Web. 01 Mar 2021.

Vancouver:

Norris LJ. Controlling Important Pathogens in Zebrafish (Danio rerio): Assessing Cryopreservation Survival of Bacteria and Parasites and Clinical Sensitivity of Mycobacterial qPCR Assays. [Internet] [Masters thesis]. Oregon State University; 2017. [cited 2021 Mar 01]. Available from: http://hdl.handle.net/1957/61867.

Council of Science Editors:

Norris LJ. Controlling Important Pathogens in Zebrafish (Danio rerio): Assessing Cryopreservation Survival of Bacteria and Parasites and Clinical Sensitivity of Mycobacterial qPCR Assays. [Masters Thesis]. Oregon State University; 2017. Available from: http://hdl.handle.net/1957/61867

4. Sarantis, Panagiotis. Μελέτη μηχανισμών καρδιοπαθειών χρησιμοποιώντας το Zebrafish ως πειραματικό μοντέλο.

Degree: 2020, National and Kapodistrian University of Athens; Εθνικό και Καποδιστριακό Πανεπιστήμιο Αθηνών (ΕΚΠΑ)

URL: http://hdl.handle.net/10442/hedi/47888

► Cardiovascular diseases are nowadays one of the leading causes of death in the developed world. Also very important is the contribution of angiogenesis to the… (more)

▼ Cardiovascular diseases are nowadays one of the leading causes of death in the developed world. Also very important is the contribution of angiogenesis to the development of cancerous tumors. It is therefore necessary to contribute to the basic research in order to better study the mechanisms of these diseases but also to discover new drugs related to angiogenesis. In recent years, the zebrafish animal model has played an important role in these conditions. Zebrafish (Danio rerio) is widely used as an animal model to understand the pathophysiology of cardiovascular diseases. Here, we present the adult cardiac phenotype of weak atrium, myh6-/-, which carry mutations in the zebrafish atrial myosin heavy chain. Homozygous mutants survive to adulthood and are fertile despite their initial weak atrial beat. In adult mutants, the atrium remains hypoplastic and shows elastin deposition while mutant ventricles exhibit increased size. In mammals, hypertrophy is the most common mechanism resulting in cardiomegaly. Using immunohistochemistry and confocal microscopy to measure cardiomyocyte cell size, density and proliferation, we show that the enlargement of the myh6-/- ventricle is predominantly due to hyperplasia. However, we identified similar transcriptional profiles to the mammalian hypertrophy response via RT-PCR of the hyperplastic ventricles. Furthermore, we show activation of the ER-stress pathway by western blot analysis. In conclusion, we can assume, based on our model, that molecular signaling pathways associated with hypertrophy in mammals, in combination with ER-stress activation, result in hyperplasia in zebrafish. In addition, to our knowledge, this is the first time to report elastin deposition in the atrium. In parallel, we studied small molecules that inhibit angiogenesis and are attractive drug candidates for cancer, retinopathies, and age-related macular degeneration. In vivo phenotypic screening in zebrafish (Danio rerio) emerges as a powerful methodology to identify and optimize novel compounds with pharmacological activity. Zebrafish provides several advantages for in vivo phenotypic screens especially for angiogenesis, since it develops rapidly, externally, and does not rely on a functional cardiovascular system to survive for several days during development. In this study, we utilize a transgenic line that allows the noninvasive monitoring of angiogenesis at a cellular level. The inhibition of angiogenesis can be observed under a fluorescent stereoscope and quantified. To exemplify the versatility and robustness of the zebrafish screen, we have employed a series of 60 novel compounds that were designed based on a potent VEGFR2 inhibitor. Herein, we report their structure-based design, synthesis, and in vivo zebrafish screening for optimal activity, toxicity, and off-target effects, which revealed six reversible inhibitors of angiogenesis. Finally, in the present doctoral dissertation, genes were attempted to play a role in the morphogenesis of the heart valves and to demonstrate their relationship…

Subjects/Keywords: Kαρδιά; Zebrafish; Heart; Zebrafish

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APA (6^th Edition):

Sarantis, P. (2020). Μελέτη μηχανισμών καρδιοπαθειών χρησιμοποιώντας το Zebrafish ως πειραματικό μοντέλο. (Thesis). National and Kapodistrian University of Athens; Εθνικό και Καποδιστριακό Πανεπιστήμιο Αθηνών (ΕΚΠΑ). Retrieved from http://hdl.handle.net/10442/hedi/47888

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Sarantis, Panagiotis. “Μελέτη μηχανισμών καρδιοπαθειών χρησιμοποιώντας το Zebrafish ως πειραματικό μοντέλο.” 2020. Thesis, National and Kapodistrian University of Athens; Εθνικό και Καποδιστριακό Πανεπιστήμιο Αθηνών (ΕΚΠΑ). Accessed March 01, 2021. http://hdl.handle.net/10442/hedi/47888.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Sarantis, Panagiotis. “Μελέτη μηχανισμών καρδιοπαθειών χρησιμοποιώντας το Zebrafish ως πειραματικό μοντέλο.” 2020. Web. 01 Mar 2021.

Vancouver:

Sarantis P. Μελέτη μηχανισμών καρδιοπαθειών χρησιμοποιώντας το Zebrafish ως πειραματικό μοντέλο. [Internet] [Thesis]. National and Kapodistrian University of Athens; Εθνικό και Καποδιστριακό Πανεπιστήμιο Αθηνών (ΕΚΠΑ); 2020. [cited 2021 Mar 01]. Available from: http://hdl.handle.net/10442/hedi/47888.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Sarantis P. Μελέτη μηχανισμών καρδιοπαθειών χρησιμοποιώντας το Zebrafish ως πειραματικό μοντέλο. [Thesis]. National and Kapodistrian University of Athens; Εθνικό και Καποδιστριακό Πανεπιστήμιο Αθηνών (ΕΚΠΑ); 2020. Available from: http://hdl.handle.net/10442/hedi/47888

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Waikato

5. Ahmadi, Ramtin. Eating behaviour regulation in Zebrafish (Danio rerio): a comparative analysis .

Degree: 2014, University of Waikato

URL: http://hdl.handle.net/10289/8791

► The structure, function, and tissue specific expression profiles of neuropeptides involved in regulating eating behaviour are well researched in mammals, the corresponding literature on fish… (more)

▼ The structure, function, and tissue specific expression profiles of neuropeptides involved in regulating eating behaviour are well researched in mammals, the corresponding literature on fish homologs however is scarce. The work presented here endeavoured to characterise the full coding sequences of peptide homologs in zebrafish as well as the expression profiles of said peptides under different energy states to elucidate their function in fish. Zebrafish were chosen as a model to represent fish species as they are amenable to molecular study. Conducting a literature and TBLASTn search, genes were identified in zebrafish that are known to be involved in affecting appetite and satiety in other vertebrates. Comparison of the translated sequences of these genes against fish and other vertebrates provided confidence in the identity of AGRP, GHRL, POMCb, CART1, CART3, and CART4 and all genes showed homology against mammalian transcripts through structural and syntenic comparison. RACE synthesis of the full coding sequence of these genes was attempted from isolated brain mRNA but only resulted in the production of a partial 3’ sequence for CART1 and CART4 which showed high identity against the predicted nucleotide but not the translated sequence. To observe the functional properties of a suite of genes, a qPCR analysis of the expression profiles of AGRP, GHRL, POMCa, POMCb, CART1, CART2, CART3, CART4, OXT, NPY, and AVPwas undertaken in 24 hour fasted zebrafish. The results were comparable to both other fish and mammals as AGRP was orexigenically upregulated in fasted zebrafish, while POMCb and OXT were anorexigenically downregulated. This is the first time that an expression study has been conducted across all of these genes together in a single species and, while the results are preliminary, they outline that the simultaneous analysis of the many genes thought to be involved in eating behaviour is a viable approach to discovering the functional roles of the range of genes, their interaction with each other, and how that relates to eating behaviour and provides some confidence in use of zebrafish as a model to highlight this. Advisors/Committee Members: Bird, Steve (advisor), Olszewski, Pawel K (advisor).

Subjects/Keywords: Zebrafish; feeding

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Ahmadi, R. (2014). Eating behaviour regulation in Zebrafish (Danio rerio): a comparative analysis . (Masters Thesis). University of Waikato. Retrieved from http://hdl.handle.net/10289/8791

Chicago Manual of Style (16^th Edition):

Ahmadi, Ramtin. “Eating behaviour regulation in Zebrafish (Danio rerio): a comparative analysis .” 2014. Masters Thesis, University of Waikato. Accessed March 01, 2021. http://hdl.handle.net/10289/8791.

MLA Handbook (7^th Edition):

Ahmadi, Ramtin. “Eating behaviour regulation in Zebrafish (Danio rerio): a comparative analysis .” 2014. Web. 01 Mar 2021.

Vancouver:

Ahmadi R. Eating behaviour regulation in Zebrafish (Danio rerio): a comparative analysis . [Internet] [Masters thesis]. University of Waikato; 2014. [cited 2021 Mar 01]. Available from: http://hdl.handle.net/10289/8791.

Council of Science Editors:

Ahmadi R. Eating behaviour regulation in Zebrafish (Danio rerio): a comparative analysis . [Masters Thesis]. University of Waikato; 2014. Available from: http://hdl.handle.net/10289/8791

University of Manchester

6. Oltrabella, Francesca. INVESTIGATION OF OCRL1 AND ITS INTERACTION PARTNERS IN ZEBRAFISH.

Degree: 2014, University of Manchester

URL: http://www.manchester.ac.uk/escholar/uk-ac-man-scw:240811

►

Oculocerebrorenal syndrome of Lowe is a rare X-linked disorder caused bymutation of the inositol 5-phosphatase OCRL1. Lowe Syndrome manifests as renaltubular dysfunction, neurological and ocular… (more)

▼

Oculocerebrorenal syndrome of Lowe is a rare X-linked disorder caused bymutation of the inositol 5-phosphatase OCRL1. Lowe Syndrome manifests as renaltubular dysfunction, neurological and ocular defects. OCRL1 uses its catalyticdomain to hydrolyze two phosphoinositide species, PI(4,5)P2 and PI3,4,5)P3. It isinvolved in regulation of membrane trafficking, actin dynamics, cytokinesis andciliogenesis. OCRL1 interacts with IPIP27A and B, which have been shown to bekey players in endocytic trafficking in mammalian cells, specifically in the recyclingof proteins from early and recycling endosomes to both the plasma membrane andtrans-Golgi network. It has been proposed that defective endocytic trafficking maybe responsible for the renal tubulopathy seen in Lowe Syndrome patients,characterized by low molecular weight proteinuria and aminoaciduria, but thishypothesis has yet to be tested.Using zebrafish as a model for Lowe syndrome, we show that depletion ofOcrl1 can indeed cause defects in endocytosis in the renal tubule. This coincideswith a reduction in levels of the multi-ligand receptor megalin, reduced abundanceof the endocytic apparatus and increased numbers of enlarged lysosomes in thekidney tubular cells. We also show that knocking-down Pip5K in the Ocrl1 mutantsto rebalance PI(4,5)P2 levels can rescue the endocytic defect. This indicates thattight control of PI(4,5)P2 level is essential for efficient endocytic trafficking in vivo.Importantly, this finding suggests that Pip5K may be a valuable therapeutic targetfor patients with Lowe Syndrome.To further characterize the molecular mechanisms by which OCRL1 promotesendocytosis, we have focused on the recently identified Ocrl1 interaction partnersIPIP27A and B, which are known to function in endocytosis and receptor recycling.Here we report identification and characterization of the zebrafish Ipip27s, includinganalysis of conservation and expression profiles. To assess Ipip27s function invivo, KO zebrafish lines were generated using TALENs. This was successful forIpip27A, but so far not for Ipip27B. Functional analysis using the Ipip27A KO lineand KD with morpholinos revealed that both Ipip27s contribute to neuraldevelopment and may participate in ciliogenesis. Moreover, preliminary analysisindicates an important role for Ipip27A within the endocytic pathway in the kidneytubule, where its loss phenocopies many aspects of the Ocrl1 mutant phenotype.

CD - MOVIE 1 AND MOVIE 2

Advisors/Committee Members: HURLSTONE, ADAM AFL, Hurlstone, Adam, Lowe, Martin.

Subjects/Keywords: OCRL1; ZEBRAFISH

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Oltrabella, F. (2014). INVESTIGATION OF OCRL1 AND ITS INTERACTION PARTNERS IN ZEBRAFISH. (Doctoral Dissertation). University of Manchester. Retrieved from http://www.manchester.ac.uk/escholar/uk-ac-man-scw:240811

Chicago Manual of Style (16^th Edition):

Oltrabella, Francesca. “INVESTIGATION OF OCRL1 AND ITS INTERACTION PARTNERS IN ZEBRAFISH.” 2014. Doctoral Dissertation, University of Manchester. Accessed March 01, 2021. http://www.manchester.ac.uk/escholar/uk-ac-man-scw:240811.

MLA Handbook (7^th Edition):

Oltrabella, Francesca. “INVESTIGATION OF OCRL1 AND ITS INTERACTION PARTNERS IN ZEBRAFISH.” 2014. Web. 01 Mar 2021.

Vancouver:

Oltrabella F. INVESTIGATION OF OCRL1 AND ITS INTERACTION PARTNERS IN ZEBRAFISH. [Internet] [Doctoral dissertation]. University of Manchester; 2014. [cited 2021 Mar 01]. Available from: http://www.manchester.ac.uk/escholar/uk-ac-man-scw:240811.

Council of Science Editors:

Oltrabella F. INVESTIGATION OF OCRL1 AND ITS INTERACTION PARTNERS IN ZEBRAFISH. [Doctoral Dissertation]. University of Manchester; 2014. Available from: http://www.manchester.ac.uk/escholar/uk-ac-man-scw:240811

University of Adelaide

7. Lim, Anne Hwee Ling. Analysis of the subcellular localization of proteins implicated in Alzheimer’s disease.

Degree: 2015, University of Adelaide

URL: http://hdl.handle.net/2440/103503

► Alzheimer’s disease (AD) is a neurodegenerative disorder involving neuropathological changes including the presence of amyloid plaques in the brain. Amyloid plaques are comprised mainly of… (more)

▼ Alzheimer’s disease (AD) is a neurodegenerative disorder involving neuropathological changes including the presence of amyloid plaques in the brain. Amyloid plaques are comprised mainly of the Aβ peptide, formed by the cleavage of the AMYLOID BETA A4 PRECURSOR PROTEIN (APP) by the enzyme complex γ-secretase. The catalytic component of the γ-secretase complex is PRESENILIN (PSEN1 or PSEN2). Mutations in the human PSEN1 gene have been identified in AD. Some mutations have been found to cause frame shifts leading to premature stop codons that produce transcripts encoding truncated PSEN1 protein. The subcellular location of the PSENs has been controversial, with studies detecting these proteins in multiple areas in the cell. However recent research has shown that the PSENs are enriched in the mitochondria associated membranes (MAM). The zebrafish is a small freshwater fish that is a useful animal model for the study of human diseases. Zebrafish have genes that are orthologous to human genes that play a role in AD, including those that encode the components of the γ-secretase complex. However, the zebrafish orthologue of the NICASTRIN gene has not been studied in detail. In Chapter II, we characterize in zebrafish the orthologue of the human NICASTRIN (NCSTN) gene that encodes a protein component of the γ-secretase. We demonstrate the spatial and temporal expression level of zebrafish ncstn in embryos and various adult tissues. This work provides the basic knowledge required to further the understanding of the role of Ncstn in the γ-secretase complex using the zebrafish animal model. Several truncations of the human PSEN1 and zebrafish Psen1 proteins appear to have differential effects on Notch signalling and Appa cleavage. The basis for these differences is still unclear. Chapter III examines the subcellular localization of fusions of truncations of zebrafish Psen1 protein with green fluorescent protein (GFP) using a simple protocol to obtain single cells from zebrafish embryos for microscopy analysis. We show that the different truncations analysed appear to have similar distributions, suggesting that the differential effects on γ-secretase substrates are likely not due to different subcellular localisations of these truncated forms of Psen1. This chapter contributes a new method of viewing a single layer of zebrafish cells with confocal microscopy. The mitochondria-associated membranes (MAM) are a subcompartment of the endoplasmic reticulum (ER) which interacts with mitochondria. The MAM is highly enriched in PSEN1 and PSEN2. Mitochondrion-ER appositions are increased in Psen1⁻ʹ⁻/Psen2⁻ʹ⁻ mouse embryonic fibroblasts (MEFs) and also in familial AD (FAD) and sporadic AD (SAD) patient cells. These results, which were performed using mammalian cells, have not been studied in the zebrafish animal model. Therefore, Chapter IV of this thesis examines the effect of inhibiting zebrafish psen1 and psen2 activity on mitochondrion-ER appositions in 24 hpf embryos. The findings in this study differ from those done in mammalian… Advisors/Committee Members: Lardelli, Michael Trent (advisor), Kelly, Joan Maree (advisor), School of Biological Sciences (school).

Subjects/Keywords: Alzheimer’s; zebrafish

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APA (6^th Edition):

Lim, A. H. L. (2015). Analysis of the subcellular localization of proteins implicated in Alzheimer’s disease. (Thesis). University of Adelaide. Retrieved from http://hdl.handle.net/2440/103503

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Lim, Anne Hwee Ling. “Analysis of the subcellular localization of proteins implicated in Alzheimer’s disease.” 2015. Thesis, University of Adelaide. Accessed March 01, 2021. http://hdl.handle.net/2440/103503.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Lim, Anne Hwee Ling. “Analysis of the subcellular localization of proteins implicated in Alzheimer’s disease.” 2015. Web. 01 Mar 2021.

Vancouver:

Lim AHL. Analysis of the subcellular localization of proteins implicated in Alzheimer’s disease. [Internet] [Thesis]. University of Adelaide; 2015. [cited 2021 Mar 01]. Available from: http://hdl.handle.net/2440/103503.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Lim AHL. Analysis of the subcellular localization of proteins implicated in Alzheimer’s disease. [Thesis]. University of Adelaide; 2015. Available from: http://hdl.handle.net/2440/103503

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Houston

8. Zambrano Cobo, Amarayca. Inflammation in Zebrafish Models.

Degree: MS, Biomedical Engineering, 2015, University of Houston

URL: http://hdl.handle.net/10657/4848

► The zebrafish is an outstanding model for in-vivo imaging of inflammation due to its optical translucency and the ability of transgenic lines to express fluorescent… (more)

Subjects/Keywords: Zebrafish; Inflammation

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APA (6^th Edition):

Zambrano Cobo, A. (2015). Inflammation in Zebrafish Models. (Masters Thesis). University of Houston. Retrieved from http://hdl.handle.net/10657/4848

Chicago Manual of Style (16^th Edition):

Zambrano Cobo, Amarayca. “Inflammation in Zebrafish Models.” 2015. Masters Thesis, University of Houston. Accessed March 01, 2021. http://hdl.handle.net/10657/4848.

MLA Handbook (7^th Edition):

Zambrano Cobo, Amarayca. “Inflammation in Zebrafish Models.” 2015. Web. 01 Mar 2021.

Vancouver:

Zambrano Cobo A. Inflammation in Zebrafish Models. [Internet] [Masters thesis]. University of Houston; 2015. [cited 2021 Mar 01]. Available from: http://hdl.handle.net/10657/4848.

Council of Science Editors:

Zambrano Cobo A. Inflammation in Zebrafish Models. [Masters Thesis]. University of Houston; 2015. Available from: http://hdl.handle.net/10657/4848

University of Alberta

9. Brewster, Daniel L. Voltage gated potassium currents in the Mauthner and MiD2cm cells of larval zebrafish.

Degree: MS, Department of Biological Sciences, 2012, University of Alberta

URL: https://era.library.ualberta.ca/files/wh246s285

► The escape response in zebrafish is mediated in part by the Mauthner cell and its two homologues, MiD2cm and MiD3cm. In adult fish, the Mauthner… (more)

Subjects/Keywords: Mauthner; Potassium; Zebrafish

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APA (6^th Edition):

Brewster, D. L. (2012). Voltage gated potassium currents in the Mauthner and MiD2cm cells of larval zebrafish. (Masters Thesis). University of Alberta. Retrieved from https://era.library.ualberta.ca/files/wh246s285

Chicago Manual of Style (16^th Edition):

Brewster, Daniel L. “Voltage gated potassium currents in the Mauthner and MiD2cm cells of larval zebrafish.” 2012. Masters Thesis, University of Alberta. Accessed March 01, 2021. https://era.library.ualberta.ca/files/wh246s285.

MLA Handbook (7^th Edition):

Brewster, Daniel L. “Voltage gated potassium currents in the Mauthner and MiD2cm cells of larval zebrafish.” 2012. Web. 01 Mar 2021.

Vancouver:

Brewster DL. Voltage gated potassium currents in the Mauthner and MiD2cm cells of larval zebrafish. [Internet] [Masters thesis]. University of Alberta; 2012. [cited 2021 Mar 01]. Available from: https://era.library.ualberta.ca/files/wh246s285.

Council of Science Editors:

Brewster DL. Voltage gated potassium currents in the Mauthner and MiD2cm cells of larval zebrafish. [Masters Thesis]. University of Alberta; 2012. Available from: https://era.library.ualberta.ca/files/wh246s285

University of Bristol

10. Campbell, Jennie S. Investigating novel players in inflammatory cell migration to sites of tissue damage in vivo.

Degree: PhD, 2020, University of Bristol

URL: http://hdl.handle.net/1983/e5fb59ce-3715-4679-8113-97be14546369

► The use of model organisms is vital for the improvement of our understanding of dynamic immunological processes. Previous work in the developing Drosophila embryo has… (more)

▼ The use of model organisms is vital for the improvement of our understanding of dynamic immunological processes. Previous work in the developing Drosophila embryo has demonstrated that epithelial wounding by laser ablation induces the rapid production of hydrogen peroxide (H2O2). This early damage signal activates the tyrosine kinase Src42a within the embryonic hemocytes – the innate immune cells of the organism. Src42a subsequently phosphorylates the tissue damage receptor Draper on its intracellular immunoreceptor tyrosine activation motif (ITAM) domain. This recruits a second kinase – Shark – and results in the activation of hemocytes and their subsequent migration to the damage site. As kinase activation is key to the tissue damage response of these inflammatory cells, we reasoned that other hemocyte-specific proteins may also be phosphorylated following H2O2 signalling. Therefore, a phosphoproteomics screen was conducted in order to uncover novel H2O2/Src42a regulated phosphoproteins. Using this approach, we discovered a hemocyte wound recruitment defect in embryos lacking the PTP type phosphatase Pez. This was shown to be cell-autonomous; and time-lapse imaging revealed a lack of directionality within Pez mutant hemocytes in the presence of an epithelial wound. We also uncovered dynamic localisation of Pez in vivo and it’s colocalization with Draper during the engulfment of apoptotic debris. Finally, we demonstrated that the loss of Draper and Src42a leads to the mislocalisation of Pez. To investigate whether this signalling pathway is evolutionarily conserved, tail fin clipping of 3 dpf Danio rerio larvae was utilised. Excitingly, Draper orthologue (MEGF10) morphants showed a reduction in both neutrophil and macrophage numbers recruited to the wound margin. This observation was further confirmed by the generation transient CRISPant larvae. Finally, we demonstrated that the Pez orthologue – named PTPN21 – also plays a role in inflammation in the zebrafish following gene disruption by CRISPR.

Subjects/Keywords: Drosophila; Zebrafish; Inflammation

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APA (6^th Edition):

Campbell, J. S. (2020). Investigating novel players in inflammatory cell migration to sites of tissue damage in vivo. (Doctoral Dissertation). University of Bristol. Retrieved from http://hdl.handle.net/1983/e5fb59ce-3715-4679-8113-97be14546369

Chicago Manual of Style (16^th Edition):

Campbell, Jennie S. “Investigating novel players in inflammatory cell migration to sites of tissue damage in vivo.” 2020. Doctoral Dissertation, University of Bristol. Accessed March 01, 2021. http://hdl.handle.net/1983/e5fb59ce-3715-4679-8113-97be14546369.

MLA Handbook (7^th Edition):

Campbell, Jennie S. “Investigating novel players in inflammatory cell migration to sites of tissue damage in vivo.” 2020. Web. 01 Mar 2021.

Vancouver:

Campbell JS. Investigating novel players in inflammatory cell migration to sites of tissue damage in vivo. [Internet] [Doctoral dissertation]. University of Bristol; 2020. [cited 2021 Mar 01]. Available from: http://hdl.handle.net/1983/e5fb59ce-3715-4679-8113-97be14546369.

Council of Science Editors:

Campbell JS. Investigating novel players in inflammatory cell migration to sites of tissue damage in vivo. [Doctoral Dissertation]. University of Bristol; 2020. Available from: http://hdl.handle.net/1983/e5fb59ce-3715-4679-8113-97be14546369

University of Saskatchewan

11. Kallarakavumkal Thomas, Jith. Effects of dietary and in ovo selenomethionine exposure in zebrafish.

Degree: 2014, University of Saskatchewan

URL: http://hdl.handle.net/10388/ETD-2014-09-1744

► Selenium (Se) is an essential trace element to most living organisms, however when compared to other ingested essential trace elements Se has the lowest margin… (more)

▼ Selenium (Se) is an essential trace element to most living organisms, however when compared to other ingested essential trace elements Se has the lowest margin of safety between essential and toxic concentrations. Oviparous vertebrates, especially fishes, are highly susceptible to dietary Se toxicity. Greater incidences of deformities and/or mortalities have been observed in F1 generation larval fishes whose parents were exposed to excess dietary Se in the form of selenomethionine (SeMet), however little information is available on effects of chronic dietary SeMet exposure to adult fish and persistent effects of in ovo SeMet exposure to F1 generation fish. This thesis investigated effects of chronic dietary exposure of excess Se in the form of SeMet on swimming performance (Ucrit), oxygen consumption (MO2), stored energy (triglycerides and glycogen), and the physiological stress response (cortisol production) in adult zebrafish (Danio rerio), as well as immediate (incidence of deformities and mortality) and persistent (e.g. changes in Ucrit, MO2, bioenergetics, the physiological stress response and reproduction) effects of in ovo exposure to SeMet in F1 generation zebrafish. In addition, the study investigated potential underlying mechanisms of SeMet-induced developmental toxicities in early life stages of zebrafish using embryo microinjection. Two separate dietary SeMet exposure studies in adult zebrafish and two in ovo SeMet maternal transfer studies in F1 generation zebrafish were conducted. The first dietary or in ovo exposure study explored effects of excess SeMet exposure on adult zebrafish or the entire life cycle of F1 generation zebrafish. The second study investigated mechanisms of observed SeMet-induced effects on adult or F1 generation zebrafish. In the first feeding study, a significant reduction in Ucrit and greater accumulation of stored energy were observed in the excess dietary SeMet exposed groups when compared to the Se-sufficient dietary control group. The second feeding study showed a greater metabolic rate, and impaired aerobic energy metabolism and triglyceride homeostasis in adult fish fed excess dietary SeMet, which was associated with a reduction in swimming performance and accumulation of triglycerides. Embryos collected from adult zebrafish in both dietary SeMet exposure studies were used to investigate effects of in ovo SeMet exposure on the entire life cycle of F1 generation fish. The first study showed a greater incidence of mortality, an increasing trend for deformities in F1 generation larval zebrafish, and reduced Ucrit in F1 generation adult fish exposed to excess SeMet via in ovo maternal transfer. However, concentrations of stored energy, cortisol and reproduction were unaltered. The second study found that impaired aerobic performance might have been responsible for the reduction in Ucrit of F1 generation adult zebrafish exposed to excess SeMet. Since there is a high variability in Se deposition among eggs via natural maternal transfer, SeMet embryo microinjection… Advisors/Committee Members: Janz, David M., Blakley, Barry, Krone, Pat, Niyogi, Som, Drew, Murray.

Subjects/Keywords: Selenomethionine Zebrafish Toxicity

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APA (6^th Edition):

Kallarakavumkal Thomas, J. (2014). Effects of dietary and in ovo selenomethionine exposure in zebrafish. (Thesis). University of Saskatchewan. Retrieved from http://hdl.handle.net/10388/ETD-2014-09-1744

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Kallarakavumkal Thomas, Jith. “Effects of dietary and in ovo selenomethionine exposure in zebrafish.” 2014. Thesis, University of Saskatchewan. Accessed March 01, 2021. http://hdl.handle.net/10388/ETD-2014-09-1744.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Kallarakavumkal Thomas, Jith. “Effects of dietary and in ovo selenomethionine exposure in zebrafish.” 2014. Web. 01 Mar 2021.

Vancouver:

Kallarakavumkal Thomas J. Effects of dietary and in ovo selenomethionine exposure in zebrafish. [Internet] [Thesis]. University of Saskatchewan; 2014. [cited 2021 Mar 01]. Available from: http://hdl.handle.net/10388/ETD-2014-09-1744.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Kallarakavumkal Thomas J. Effects of dietary and in ovo selenomethionine exposure in zebrafish. [Thesis]. University of Saskatchewan; 2014. Available from: http://hdl.handle.net/10388/ETD-2014-09-1744

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

12. Boyle, Cody Adam. Intergenerational Effects Of Embryonic Cocaine Exposure In Zebrafish.

Degree: MS, Biology, 2017, University of North Dakota

URL: https://commons.und.edu/theses/2174

► Cocaine addiction has both genetic and environmentally driven components. Relatively recently several groups have suggested that epigenetic regulation of gene expression might be the… (more)

Subjects/Keywords: Cocaine; Intergenerational; Zebrafish

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APA (6^th Edition):

Boyle, C. A. (2017). Intergenerational Effects Of Embryonic Cocaine Exposure In Zebrafish. (Masters Thesis). University of North Dakota. Retrieved from https://commons.und.edu/theses/2174

Chicago Manual of Style (16^th Edition):

Boyle, Cody Adam. “Intergenerational Effects Of Embryonic Cocaine Exposure In Zebrafish.” 2017. Masters Thesis, University of North Dakota. Accessed March 01, 2021. https://commons.und.edu/theses/2174.

MLA Handbook (7^th Edition):

Boyle, Cody Adam. “Intergenerational Effects Of Embryonic Cocaine Exposure In Zebrafish.” 2017. Web. 01 Mar 2021.

Vancouver:

Boyle CA. Intergenerational Effects Of Embryonic Cocaine Exposure In Zebrafish. [Internet] [Masters thesis]. University of North Dakota; 2017. [cited 2021 Mar 01]. Available from: https://commons.und.edu/theses/2174.

Council of Science Editors:

Boyle CA. Intergenerational Effects Of Embryonic Cocaine Exposure In Zebrafish. [Masters Thesis]. University of North Dakota; 2017. Available from: https://commons.und.edu/theses/2174

University of Ottawa

13. Boratynska, Susan. Biological Confinement of Zebrafish Using RNAi .

Degree: 2015, University of Ottawa

URL: http://hdl.handle.net/10393/32775

► The increasing demand for fish in the food industry has resulted in the extensive overfishing of wild fisheries. In efforts to alleviate the demand from… (more)

▼ The increasing demand for fish in the food industry has resulted in the extensive overfishing of wild fisheries. In efforts to alleviate the demand from the food industry, genetically modified (GM) fish were developed possessing traits such as larger mass, faster growth, and increased resistance to disease. However, the greater fitness advantage of GM fish presents potential risks for wild type populations in the event of release or escape from a confined fish facility. In addition to physical barriers, it is critical to develop a genetic mechanism in order to ensure that the spread of GM transgene to the natural populations does not occur. RNA interference (RNAi) is an endogenous mechanism used to regulate gene expression by destroying targeted mRNA molecules. Manipulation of this biological process has been successfully utilized to knockdown specific genes through the introduction of synthetic transgenes in organisms such as C. elegans, M. musculus, and D. melanogaster. Although the use of RNAi as a biological tool is still relatively new in zebrafish, recent work has explored elements of this mechanism allowing for greater knockdown efficiencies. The deadend (dnd) gene is required for primordial germ cell (PGC) development and survival. Previous studies have shown that zebrafish dnd knockouts develop into sterile adults without disrupting somatic development. In efforts to induce sterility in zebrafish, short hairpin RNA (shRNA) constructs targeting dnd were designed to exploit the endogenous RNAi pathway. Upon qualitative analysis in transient and transgenic zebrafish subjected to the synthetic RNAi construct, a reduction in the germ cell population at early stages of development was observed. However, quantification of dnd mRNA in fish from the same time points did not show significant changes in expression levels compared to their wildtype counterparts. Adult fish subjected to the transgene construct produced viable gametes. The use of RNAi as a tool for bioconfinement relies on sterility among all individuals subjected to the shRNA bearing transgene. Based on the results obtained, the verdict is still unclear as to whether shRNA is a viable mechanism for large scale bioconfinement.

Subjects/Keywords: RNAi; dnd; Zebrafish

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Boratynska, S. (2015). Biological Confinement of Zebrafish Using RNAi . (Thesis). University of Ottawa. Retrieved from http://hdl.handle.net/10393/32775

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Boratynska, Susan. “Biological Confinement of Zebrafish Using RNAi .” 2015. Thesis, University of Ottawa. Accessed March 01, 2021. http://hdl.handle.net/10393/32775.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Boratynska, Susan. “Biological Confinement of Zebrafish Using RNAi .” 2015. Web. 01 Mar 2021.

Vancouver:

Boratynska S. Biological Confinement of Zebrafish Using RNAi . [Internet] [Thesis]. University of Ottawa; 2015. [cited 2021 Mar 01]. Available from: http://hdl.handle.net/10393/32775.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Boratynska S. Biological Confinement of Zebrafish Using RNAi . [Thesis]. University of Ottawa; 2015. Available from: http://hdl.handle.net/10393/32775

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Deakin University

14. Mohd Noor, Suzita. Role of Jak2/Stat5/Cis pathway components in zebrafish development.

Degree: School of Medicine, 2011, Deakin University

URL: http://hdl.handle.net/10536/DRO/DU:30036712

► By utilizing the zebrafish as a research model, the function of specific cell signalling pathway components in development was investigated. This revealed new roles for… (more)

Subjects/Keywords: embryology; cytokines; zebrafish

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APA (6^th Edition):

Mohd Noor, S. (2011). Role of Jak2/Stat5/Cis pathway components in zebrafish development. (Thesis). Deakin University. Retrieved from http://hdl.handle.net/10536/DRO/DU:30036712

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Mohd Noor, Suzita. “Role of Jak2/Stat5/Cis pathway components in zebrafish development.” 2011. Thesis, Deakin University. Accessed March 01, 2021. http://hdl.handle.net/10536/DRO/DU:30036712.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Mohd Noor, Suzita. “Role of Jak2/Stat5/Cis pathway components in zebrafish development.” 2011. Web. 01 Mar 2021.

Vancouver:

Mohd Noor S. Role of Jak2/Stat5/Cis pathway components in zebrafish development. [Internet] [Thesis]. Deakin University; 2011. [cited 2021 Mar 01]. Available from: http://hdl.handle.net/10536/DRO/DU:30036712.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Mohd Noor S. Role of Jak2/Stat5/Cis pathway components in zebrafish development. [Thesis]. Deakin University; 2011. Available from: http://hdl.handle.net/10536/DRO/DU:30036712

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Manchester

15. Oltrabella, Francesca. Investigation of OCRL1 and its interaction partners in zebrafish.

Degree: PhD, 2014, University of Manchester

URL: https://www.research.manchester.ac.uk/portal/en/theses/investigation-of-ocrl1-and-its-interaction-partners-in-zebrafish(77a97cd8-f030-4c4e-93cc-fe4e2ea44ee6).html ; https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.764293

► Oculocerebrorenal syndrome of Lowe is a rare X-linked disorder caused by mutation of the inositol 5-phosphatase OCRL1. Lowe Syndrome manifests as renal tubular dysfunction, neurological… (more)

▼ Oculocerebrorenal syndrome of Lowe is a rare X-linked disorder caused by mutation of the inositol 5-phosphatase OCRL1. Lowe Syndrome manifests as renal tubular dysfunction, neurological and ocular defects. OCRL1 uses its catalytic domain to hydrolyze two phosphoinositide species, PI(4,5)P2 and PI3,4,5)P3. It is involved in regulation of membrane trafficking, actin dynamics, cytokinesis and ciliogenesis. OCRL1 interacts with IPIP27A and B, which have been shown to be key players in endocytic trafficking in mammalian cells, specifically in the recycling of proteins from early and recycling endosomes to both the plasma membrane and trans-Golgi network. It has been proposed that defective endocytic trafficking may be responsible for the renal tubulopathy seen in Lowe Syndrome patients, characterized by low molecular weight proteinuria and aminoaciduria, but this hypothesis has yet to be tested. Using zebrafish as a model for Lowe syndrome, we show that depletion of OCRL1 can indeed cause defects in endocytosis in the renal tubule. This coincides with a reduction in levels of the multi-ligand receptor megalin, reduced abundance of the endocytic apparatus and increased numbers of enlarged lysosomes in the kidney tubular cells. We also show that knocking-down Pip5K in the OCRL1 mutants to rebalance PI(4,5)P2 levels can rescue the endocytic defect. This indicates that tight control of PI(4,5)P2 level is essential for efficient endocytic trafficking in vivo. Importantly, this finding suggests that Pip5K may be a valuable therapeutic target for patients with Lowe Syndrome. To further characterize the molecular mechanisms by which OCRL1 promotes endocytosis, we have focused on the recently identified OCRL1 interaction partners IPIP27A and B, which are known to function in endocytosis and receptor recycling. Here we report identification and characterization of the zebrafish Ipip27s, including analysis of conservation and expression profiles. To assess Ipip27s function in vivo, KO zebrafish lines were generated using TALENs. This was successful for Ipip27A, but so far not for Ipip27B. Functional analysis using the Ipip27A KO line and KD with morpholinos revealed that both Ipip27s contribute to neural development and may participate in ciliogenesis. Moreover, preliminary analysis indicates an important role for Ipip27A within the endocytic pathway in the kidney tubule, where its loss phenocopies many aspects of the OCRL1 mutant phenotype.

Subjects/Keywords: 570; ZEBRAFISH; OCRL1

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APA (6^th Edition):

Oltrabella, F. (2014). Investigation of OCRL1 and its interaction partners in zebrafish. (Doctoral Dissertation). University of Manchester. Retrieved from https://www.research.manchester.ac.uk/portal/en/theses/investigation-of-ocrl1-and-its-interaction-partners-in-zebrafish(77a97cd8-f030-4c4e-93cc-fe4e2ea44ee6).html ; https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.764293

Chicago Manual of Style (16^th Edition):

Oltrabella, Francesca. “Investigation of OCRL1 and its interaction partners in zebrafish.” 2014. Doctoral Dissertation, University of Manchester. Accessed March 01, 2021. https://www.research.manchester.ac.uk/portal/en/theses/investigation-of-ocrl1-and-its-interaction-partners-in-zebrafish(77a97cd8-f030-4c4e-93cc-fe4e2ea44ee6).html ; https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.764293.

MLA Handbook (7^th Edition):

Oltrabella, Francesca. “Investigation of OCRL1 and its interaction partners in zebrafish.” 2014. Web. 01 Mar 2021.

Vancouver:

Oltrabella F. Investigation of OCRL1 and its interaction partners in zebrafish. [Internet] [Doctoral dissertation]. University of Manchester; 2014. [cited 2021 Mar 01]. Available from: https://www.research.manchester.ac.uk/portal/en/theses/investigation-of-ocrl1-and-its-interaction-partners-in-zebrafish(77a97cd8-f030-4c4e-93cc-fe4e2ea44ee6).html ; https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.764293.

Council of Science Editors:

Oltrabella F. Investigation of OCRL1 and its interaction partners in zebrafish. [Doctoral Dissertation]. University of Manchester; 2014. Available from: https://www.research.manchester.ac.uk/portal/en/theses/investigation-of-ocrl1-and-its-interaction-partners-in-zebrafish(77a97cd8-f030-4c4e-93cc-fe4e2ea44ee6).html ; https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.764293

Rice University

16. Rivera Longsworth, Gia Francesca. Expanding the Enzymatic Activity of the Programmable Endonuclease Cas9.

Degree: MA, Natural Sciences, 2018, Rice University

URL: http://hdl.handle.net/1911/105841

► The CRISPR-Cas9 gene editing system has revolutionized our ability to make targeted mutations in a variety of organisms. In zebrafish, we are able to use… (more)

▼ The CRISPR-Cas9 gene editing system has revolutionized our ability to make targeted mutations in a variety of organisms. In zebrafish, we are able to use microinjections of Cas9 protein or mRNA alongside guide RNAs (gRNA) to create targeted insertions and deletions (indels) at a frequency sufficient for recovery of loss of function alleles. Combined with the many practical advantages of working with zebrafish, CRISPR-Cas9 has enabled many labs to study gene function at a lowered cost. However, there are improvements that can be made to facilitate the knock in of sequences into the genome, a process which remains inefficient in zebrafish due to a low frequency of homology directed repair (HDR), a mechanism necessary for knock in. Our aim is to increase the rate of HDR in zebrafish by modifying Cas9 using two approaches. First, we hypothesize that the long latency period of the Cas9-DNA complex taken with the short cell cycle of the early zebrafish embryo is biasing the repair mechanism against HDR, so I have engineered a CL1 degron-tagged Cas9 (DegCas9) to destabilize the Cas9 protein. A second approach to increasing the rate of knock in is to reduce the number of molecules involved in the HDR process by using an RNA template for recombination. We hypothesize that the rate of HDR can be improved if the recombination template remains in proximity to the DSB. To this aim, I have created a reverse-transcriptase Cas9 fusion and modified gRNAs that include the targeting sequence for recombination. Our goal is to create a Cas9 system that efficiently uses an RNA template for recombination. Results indicate that DegCas9 is capable of inducing mutations at a reduced rate, and that the modified gRNAs for the RTCas9 system do not reduce the effectiveness of Cas9 function. Further studies will include analyzing the spectrum of lesions induced by DegCas9 and creating alternative versions of DegCas9 and RTCas9 using different domains. If successful, these Cas9 constructs will expand the toolkit available for genetic engineering in zebrafish. Advisors/Committee Members: Wagner, Daniel (advisor).

Subjects/Keywords: zebrafish; Cas9; CRISPR

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Rivera Longsworth, G. F. (2018). Expanding the Enzymatic Activity of the Programmable Endonuclease Cas9. (Masters Thesis). Rice University. Retrieved from http://hdl.handle.net/1911/105841

Chicago Manual of Style (16^th Edition):

Rivera Longsworth, Gia Francesca. “Expanding the Enzymatic Activity of the Programmable Endonuclease Cas9.” 2018. Masters Thesis, Rice University. Accessed March 01, 2021. http://hdl.handle.net/1911/105841.

MLA Handbook (7^th Edition):

Rivera Longsworth, Gia Francesca. “Expanding the Enzymatic Activity of the Programmable Endonuclease Cas9.” 2018. Web. 01 Mar 2021.

Vancouver:

Rivera Longsworth GF. Expanding the Enzymatic Activity of the Programmable Endonuclease Cas9. [Internet] [Masters thesis]. Rice University; 2018. [cited 2021 Mar 01]. Available from: http://hdl.handle.net/1911/105841.

Council of Science Editors:

Rivera Longsworth GF. Expanding the Enzymatic Activity of the Programmable Endonuclease Cas9. [Masters Thesis]. Rice University; 2018. Available from: http://hdl.handle.net/1911/105841

University of Arizona

17. Todd, Douglas Wallace. Zebrafish Video Analysis System for High-Throughput Drug Assay .

Degree: 2016, University of Arizona

URL: http://hdl.handle.net/10150/623150

► Zebrafish swimming behavior is used in a new, automated drug assay system as a biomarker to measure drug efficiency to prevent or restore hearing loss.… (more)

Subjects/Keywords: zebrafish; image analysis

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APA (6^th Edition):

Todd, D. W. (2016). Zebrafish Video Analysis System for High-Throughput Drug Assay . (Masters Thesis). University of Arizona. Retrieved from http://hdl.handle.net/10150/623150

Chicago Manual of Style (16^th Edition):

Todd, Douglas Wallace. “Zebrafish Video Analysis System for High-Throughput Drug Assay .” 2016. Masters Thesis, University of Arizona. Accessed March 01, 2021. http://hdl.handle.net/10150/623150.

MLA Handbook (7^th Edition):

Todd, Douglas Wallace. “Zebrafish Video Analysis System for High-Throughput Drug Assay .” 2016. Web. 01 Mar 2021.

Vancouver:

Todd DW. Zebrafish Video Analysis System for High-Throughput Drug Assay . [Internet] [Masters thesis]. University of Arizona; 2016. [cited 2021 Mar 01]. Available from: http://hdl.handle.net/10150/623150.

Council of Science Editors:

Todd DW. Zebrafish Video Analysis System for High-Throughput Drug Assay . [Masters Thesis]. University of Arizona; 2016. Available from: http://hdl.handle.net/10150/623150

University of North Texas

18. Adhikari, Prem R. Proteomic Responses in the Gill of Zebrafish Following Exposure to Ibuprofen and Naproxen.

Degree: 2012, University of North Texas

URL: https://digital.library.unt.edu/ark:/67531/metadc149557/

► Non-steroidal anti-inflammatory drugs (NSAIDs) are among the most abundant environmental pharmaceutical contaminants. In this study, a proteomic analysis was conducted to identify proteins differentially expressed… (more)

Subjects/Keywords: Proteomics; NSAID; zebrafish

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APA (6^th Edition):

Adhikari, P. R. (2012). Proteomic Responses in the Gill of Zebrafish Following Exposure to Ibuprofen and Naproxen. (Thesis). University of North Texas. Retrieved from https://digital.library.unt.edu/ark:/67531/metadc149557/

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Adhikari, Prem R. “Proteomic Responses in the Gill of Zebrafish Following Exposure to Ibuprofen and Naproxen.” 2012. Thesis, University of North Texas. Accessed March 01, 2021. https://digital.library.unt.edu/ark:/67531/metadc149557/.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Adhikari, Prem R. “Proteomic Responses in the Gill of Zebrafish Following Exposure to Ibuprofen and Naproxen.” 2012. Web. 01 Mar 2021.

Vancouver:

Adhikari PR. Proteomic Responses in the Gill of Zebrafish Following Exposure to Ibuprofen and Naproxen. [Internet] [Thesis]. University of North Texas; 2012. [cited 2021 Mar 01]. Available from: https://digital.library.unt.edu/ark:/67531/metadc149557/.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Adhikari PR. Proteomic Responses in the Gill of Zebrafish Following Exposure to Ibuprofen and Naproxen. [Thesis]. University of North Texas; 2012. Available from: https://digital.library.unt.edu/ark:/67531/metadc149557/

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of North Texas

19. Alsrhani, Abdullah Falleh. Novel Role of Trypsin in Zebrafish.

Degree: 2013, University of North Texas

URL: https://digital.library.unt.edu/ark:/67531/metadc271771/

► It has been shown previously in our laboratory that zebrafish produce trypsin from their gills when they are under stress, and this trypsin is involved… (more)

Subjects/Keywords: Trypsin; zebrafish; thrombocyte

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APA (6^th Edition):

Alsrhani, A. F. (2013). Novel Role of Trypsin in Zebrafish. (Thesis). University of North Texas. Retrieved from https://digital.library.unt.edu/ark:/67531/metadc271771/

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Alsrhani, Abdullah Falleh. “Novel Role of Trypsin in Zebrafish.” 2013. Thesis, University of North Texas. Accessed March 01, 2021. https://digital.library.unt.edu/ark:/67531/metadc271771/.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Alsrhani, Abdullah Falleh. “Novel Role of Trypsin in Zebrafish.” 2013. Web. 01 Mar 2021.

Vancouver:

Alsrhani AF. Novel Role of Trypsin in Zebrafish. [Internet] [Thesis]. University of North Texas; 2013. [cited 2021 Mar 01]. Available from: https://digital.library.unt.edu/ark:/67531/metadc271771/.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Alsrhani AF. Novel Role of Trypsin in Zebrafish. [Thesis]. University of North Texas; 2013. Available from: https://digital.library.unt.edu/ark:/67531/metadc271771/

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Oregon

20. Schlomann, Brandon. Learning Biophysical Rules of Gut Bacterial Communities Through Live Imaging of Zebrafish.

Degree: PhD, Department of Physics, 2020, University of Oregon

URL: https://scholarsbank.uoregon.edu/xmlui/handle/1794/25611

► Vast communities of microorganisms inhabit the gastrointestinal tracts of humans and other animals, where they influence diverse aspects of animal health and disease. Our understanding… (more)

▼ Vast communities of microorganisms inhabit the gastrointestinal tracts of humans and other animals, where they influence diverse aspects of animal health and disease. Our understanding of the types of microbes present in the intestine and the genes that they carry has grown tremendously in recent years, but despite this progress, we are still unable to predict the abundances of microbial strains in the gut and their impact on host phenotypes. This deficiency limits our abilities to uncover causal mechanisms mediating host-microbe interactions and to rationally design novel therapeutic strategies. A major barrier to achieving these goals is our limited ability to experimentally probe the spatial organization of gut bacterial communities, which is thought to be a key driver of microbiota dynamics, but which is largely inaccessible in most systems. This dissertation work addresses these knowledge gaps by combining quantitative theory with controlled experiments in a model system that can uniquely surmount these technical challenges. The larval zebrafish is an optically transparent, model vertebrate that is amenable to live imaging studies, in which bacteria in the gut can be directly visualized and studied in situ. Through this approach, we discovered that the biophysical properties of bacteria in the gut, especially their aggregation and swimming behaviors, coupled to intestinal fluid flows, determine in robust but probabilistic ways several large-scale features of whole bacterial populations. These features include global spatial distributions of bacteria throughout the gut, bacterial population dynamics, both at baseline and in response to perturbations like antibiotics, and the ability of bacteria to stimulate immune responses. Through the study and validation of phenomenological models, we argue that these effects are generic and manifest in other animals, including humans, and suggest new strategies to harness these effects for precision microbiome engineering. Advisors/Committee Members: Parthasarathy, Raghuveer (advisor).

Subjects/Keywords: bacteria; microbiota; zebrafish

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APA (6^th Edition):

Schlomann, B. (2020). Learning Biophysical Rules of Gut Bacterial Communities Through Live Imaging of Zebrafish. (Doctoral Dissertation). University of Oregon. Retrieved from https://scholarsbank.uoregon.edu/xmlui/handle/1794/25611

Chicago Manual of Style (16^th Edition):

Schlomann, Brandon. “Learning Biophysical Rules of Gut Bacterial Communities Through Live Imaging of Zebrafish.” 2020. Doctoral Dissertation, University of Oregon. Accessed March 01, 2021. https://scholarsbank.uoregon.edu/xmlui/handle/1794/25611.

MLA Handbook (7^th Edition):

Schlomann, Brandon. “Learning Biophysical Rules of Gut Bacterial Communities Through Live Imaging of Zebrafish.” 2020. Web. 01 Mar 2021.

Vancouver:

Schlomann B. Learning Biophysical Rules of Gut Bacterial Communities Through Live Imaging of Zebrafish. [Internet] [Doctoral dissertation]. University of Oregon; 2020. [cited 2021 Mar 01]. Available from: https://scholarsbank.uoregon.edu/xmlui/handle/1794/25611.

Council of Science Editors:

Schlomann B. Learning Biophysical Rules of Gut Bacterial Communities Through Live Imaging of Zebrafish. [Doctoral Dissertation]. University of Oregon; 2020. Available from: https://scholarsbank.uoregon.edu/xmlui/handle/1794/25611

University of Bristol

21. Campbell, Jennie S. Investigating novel players in inflammatory cell migration to sites of tissue damage in vivo.

Degree: PhD, 2020, University of Bristol

URL: https://research-information.bris.ac.uk/en/studentTheses/e5fb59ce-3715-4679-8113-97be14546369 ; https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.801589

► The use of model organisms is vital for the improvement of our understanding of dynamic immunological processes. Previous work in the developing Drosophila embryo has… (more)

▼ The use of model organisms is vital for the improvement of our understanding of dynamic immunological processes. Previous work in the developing Drosophila embryo has demonstrated that epithelial wounding by laser ablation induces the rapid production of hydrogen peroxide (H2O2). This early damage signal activates the tyrosine kinase Src42a within the embryonic hemocytes – the innate immune cells of the organism. Src42a subsequently phosphorylates the tissue damage receptor Draper on its intracellular immunoreceptor tyrosine activation motif (ITAM) domain. This recruits a second kinase – Shark – and results in the activation of hemocytes and their subsequent migration to the damage site. As kinase activation is key to the tissue damage response of these inflammatory cells, we reasoned that other hemocyte-specific proteins may also be phosphorylated following H2O2 signalling. Therefore, a phosphoproteomics screen was conducted in order to uncover novel H2O2/Src42a regulated phosphoproteins. Using this approach, we discovered a hemocyte wound recruitment defect in embryos lacking the PTP type phosphatase Pez. This was shown to be cell-autonomous; and time-lapse imaging revealed a lack of directionality within Pez mutant hemocytes in the presence of an epithelial wound. We also uncovered dynamic localisation of Pez in vivo and it’s colocalization with Draper during the engulfment of apoptotic debris. Finally, we demonstrated that the loss of Draper and Src42a leads to the mislocalisation of Pez. To investigate whether this signalling pathway is evolutionarily conserved, tail fin clipping of 3 dpf Danio rerio larvae was utilised. Excitingly, Draper orthologue (MEGF10) morphants showed a reduction in both neutrophil and macrophage numbers recruited to the wound margin. This observation was further confirmed by the generation transient CRISPant larvae. Finally, we demonstrated that the Pez orthologue – named PTPN21 – also plays a role in inflammation in the zebrafish following gene disruption by CRISPR.

Subjects/Keywords: Drosophila; Zebrafish; Inflammation

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APA (6^th Edition):

Campbell, J. S. (2020). Investigating novel players in inflammatory cell migration to sites of tissue damage in vivo. (Doctoral Dissertation). University of Bristol. Retrieved from https://research-information.bris.ac.uk/en/studentTheses/e5fb59ce-3715-4679-8113-97be14546369 ; https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.801589

Chicago Manual of Style (16^th Edition):

Campbell, Jennie S. “Investigating novel players in inflammatory cell migration to sites of tissue damage in vivo.” 2020. Doctoral Dissertation, University of Bristol. Accessed March 01, 2021. https://research-information.bris.ac.uk/en/studentTheses/e5fb59ce-3715-4679-8113-97be14546369 ; https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.801589.

MLA Handbook (7^th Edition):

Campbell, Jennie S. “Investigating novel players in inflammatory cell migration to sites of tissue damage in vivo.” 2020. Web. 01 Mar 2021.

Vancouver:

Campbell JS. Investigating novel players in inflammatory cell migration to sites of tissue damage in vivo. [Internet] [Doctoral dissertation]. University of Bristol; 2020. [cited 2021 Mar 01]. Available from: https://research-information.bris.ac.uk/en/studentTheses/e5fb59ce-3715-4679-8113-97be14546369 ; https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.801589.

Council of Science Editors:

Campbell JS. Investigating novel players in inflammatory cell migration to sites of tissue damage in vivo. [Doctoral Dissertation]. University of Bristol; 2020. Available from: https://research-information.bris.ac.uk/en/studentTheses/e5fb59ce-3715-4679-8113-97be14546369 ; https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.801589

NSYSU

22. Mou, Yu-Zheng. CoupTF1b functions in vascular development in zebrafish.

Degree: Master, Biological Sciences, 2014, NSYSU

URL: http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0702114-013814

► Genetic programs and signaling pathways are required for proper growth and patterning of blood vessels, however, little known about the transcription factors functioning in vein… (more)

Subjects/Keywords: intersegmental vessels; CoupTF1b; angiogenesis; zebrafish

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APA (6^th Edition):

Mou, Y. (2014). CoupTF1b functions in vascular development in zebrafish. (Thesis). NSYSU. Retrieved from http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0702114-013814

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Mou, Yu-Zheng. “CoupTF1b functions in vascular development in zebrafish.” 2014. Thesis, NSYSU. Accessed March 01, 2021. http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0702114-013814.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Mou, Yu-Zheng. “CoupTF1b functions in vascular development in zebrafish.” 2014. Web. 01 Mar 2021.

Vancouver:

Mou Y. CoupTF1b functions in vascular development in zebrafish. [Internet] [Thesis]. NSYSU; 2014. [cited 2021 Mar 01]. Available from: http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0702114-013814.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Mou Y. CoupTF1b functions in vascular development in zebrafish. [Thesis]. NSYSU; 2014. Available from: http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0702114-013814

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Oregon State University

23. Chlebowski, Anna C. Utilization of the Zebrafish Model for Investigating Nitrated Polycyclic Aromatic Hydrocarbon Developmental Toxicity.

Degree: PhD, Toxicology, 2017, Oregon State University

URL: http://hdl.handle.net/1957/60687

► Polycyclic aromatic hydrocarbons (PAHs) are among the most widely known and studied environmental contaminants, originating from a range of natural and anthropogenic sources. PAHs are… (more)

▼ Polycyclic aromatic hydrocarbons (PAHs) are among the most widely known and studied environmental contaminants, originating from a range of natural and anthropogenic sources. PAHs are known to occur in the environment as complex mixtures, containing both unsubstituted PAHs, as well as a range of PAH derivatives. Among the less-studied of these derivative PAH classes are nitrated PAHs (NPAHs). NPAHs are known to form from atmospheric reactions with PAHs and can be found in the environment in a variety of matrices. Many NPAHs are known to be mutagenic, in some cases more so than the corresponding unsubstituted PAH. Less is known about the toxicity of NPAHs in whole-animal systems and for non-cancer endpoints, in particular with regard to the developmental toxicity and metabolism across a wide number of NPAH compounds, in a consistent model system. One of the major challenges in studying PAHs, and related compounds, is the high hydrophobicity and low water solubility of these compounds, which can result in losses due to partitioning of the analytes out of the aqueous phase and on to the walls of the container or exposure vessel. Numerous in vitro and in vivo models utilize plastic plates as exposure vessels, including the use of polystyrene 96-well plates for developmental toxicity testing in the developing zebrafish (Danio rerio) model. We directly measured the losses which occur due to sorption to the polystyrene plates during zebrafish testing for a set of PAHs and NPAHs. Sorptive losses in some instances were greater than fifty percent, in particular for the lower of the two exposure concentrations tested. These sorptive losses decrease the concentration of chemical available to the zebrafish embryos, and therefore impact the interpretation of dose-response toxicity data. In an attempt to create a predictive model for sorptive losses, the measured sorption was modeled against the log K[subscript ow], molecular weight, and subcooled liquid solubilities of the corresponding compounds. The correlations between subcooled liquid solubility and PAH sorption was statistically significant (p<0.05) for both concentrations tested, as well as molecular weight at the higher concentrations tested. However, none of the correlations were statistically significant for NPAH sorption, indicating a need for increased research in this area. We utilized the developing zebrafish model to investigate the developmental toxicity, and potential contributing mechanisms of action, of a suite of 27 NPAHs, as well as 10 heterocyclic PAHs (HPAHs) and 2 amino-PAHs (potential metabolites of NPAHs). Results from the toxicity screen indicate that NPAHs and HPAHs have a wide range of bioactivities in the developing zebrafish, from non-toxic at the concentrations tested to acutely toxic at sub-micromolar exposure concentrations. Activation of the aryl hydrocarbon receptor (AHR) pathway was investigated using a transgenic reporter zebrafish line and morpholino oligonucleotide knockdown to isolate specific isoforms of the AHR. The compounds… Advisors/Committee Members: Simonich, Staci L. M. (advisor), Field, Jennifer (committee member).

Subjects/Keywords: zebrafish; Polycyclic aromatic hydrocarbons – Toxicology

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Chlebowski, A. C. (2017). Utilization of the Zebrafish Model for Investigating Nitrated Polycyclic Aromatic Hydrocarbon Developmental Toxicity. (Doctoral Dissertation). Oregon State University. Retrieved from http://hdl.handle.net/1957/60687

Chicago Manual of Style (16^th Edition):

Chlebowski, Anna C. “Utilization of the Zebrafish Model for Investigating Nitrated Polycyclic Aromatic Hydrocarbon Developmental Toxicity.” 2017. Doctoral Dissertation, Oregon State University. Accessed March 01, 2021. http://hdl.handle.net/1957/60687.

MLA Handbook (7^th Edition):

Chlebowski, Anna C. “Utilization of the Zebrafish Model for Investigating Nitrated Polycyclic Aromatic Hydrocarbon Developmental Toxicity.” 2017. Web. 01 Mar 2021.

Vancouver:

Chlebowski AC. Utilization of the Zebrafish Model for Investigating Nitrated Polycyclic Aromatic Hydrocarbon Developmental Toxicity. [Internet] [Doctoral dissertation]. Oregon State University; 2017. [cited 2021 Mar 01]. Available from: http://hdl.handle.net/1957/60687.

Council of Science Editors:

Chlebowski AC. Utilization of the Zebrafish Model for Investigating Nitrated Polycyclic Aromatic Hydrocarbon Developmental Toxicity. [Doctoral Dissertation]. Oregon State University; 2017. Available from: http://hdl.handle.net/1957/60687

Universiteit Utrecht

24. Stumpf, Miriam. Interplay of PTEN subcellular localization and catalytic activities in vivo.

Degree: 2016, Universiteit Utrecht

URL: http://dspace.library.uu.nl:8080/handle/1874/334160

► This thesis describes the use of mammalian cells, S. cerevisiae and D. rerio to unravel the complex interplay of PTEN subcellular localization and catalytic activities.… (more)

▼ This thesis describes the use of mammalian cells, S. cerevisiae and D. rerio to unravel the complex interplay of PTEN subcellular localization and catalytic activities. In Chapter 1 we provide a general introduction to the PI3K/Akt(PKB)/PTEN axis, PTEN phosphatase-dependent and –independent functions and regulation of those functions through changes in protein conformation and subcellular localization. Last, we give a short résumé on the role of PTEN in human health and disease. In Chapter 2, we give an overview of developmental and pathological processes that have been successfully studied in zebrafish pten models. Further, we introduce techniques that we and others have developed for modulation of Pten expression in vivo and for the establishment of zebrafish cell lines from tumors. In Chapter 3 we performed a comprehensive mutational and functional analysis of the PTEN N-terminus. To analyze the contribution of this region to PTEN tumor suppressor function in vivo, we studied a panel of tumor-related mutations, employing S.cerevisiae and mammalian cells. We found that most tumor-related N-terminal PTEN mutations that lead to a loss of PIP3-phosphatase activity also showed impaired nuclear localization. This suggests that PIP3 catalytic activity and nuclear localization of PTEN are coordinated by the PTEN N-terminus in an overlapping manner. Our results from soft agar colony formation assays further indicated that both the PTEN PIP3 phosphatase activity and the PTEN capacity to accumulate in the nucleus were important for the full tumor suppression capacity of PTEN. In Chapter 4 we focused on the classical protein import pathway as a possible major nuclear transport mechanism for PTEN and, by immunofluorescence and confocal microscopy performed in mammalian cells, we identified importin alpha3 as a factor involved in PTEN nuclear translocation. Further, we found that the PTEN N-terminal region (residues 1-32) could mediate nuclear accumulation of PTEN through interactions with nuclear transporters. Systematic introduction of point mutations in the importin alpha 3 substrate binding pockets allowed us to further narrow down the region of importin alpha 3 that is important for nuclear accumulation of PTEN to the minor binding pocket. Using the zebrafish as a model organism, in Chapter 5 we unveil a differential requirement of Pten lipid and protein phosphatase activity during embryonic development. To this end, we performed rescue assays. We propose that the role of Pten during angiogenesis mainly consists of suppressing PI3K signaling via its lipid phosphatase activity, whereas the complex process of embryonic development requires lipid and protein phosphatase activity of Pten. In Chapter 6 we characterize the subcellular localization and the functional consquences of the expression of open conformation PTEN. The functional hyperactivity of open conformation PTEN in comparison to PTEN wild type in our model seemed to result predominantly from its enhanced recruitment to the cytoplasmic membrane. In conclusion, we… Advisors/Committee Members: Bakkers, Jeroen, den Hertog, J..

Subjects/Keywords: PTEN; Zebrafish; Cancer; Angiogenesis; Importins

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Stumpf, M. (2016). Interplay of PTEN subcellular localization and catalytic activities in vivo. (Doctoral Dissertation). Universiteit Utrecht. Retrieved from http://dspace.library.uu.nl:8080/handle/1874/334160

Chicago Manual of Style (16^th Edition):

Stumpf, Miriam. “Interplay of PTEN subcellular localization and catalytic activities in vivo.” 2016. Doctoral Dissertation, Universiteit Utrecht. Accessed March 01, 2021. http://dspace.library.uu.nl:8080/handle/1874/334160.

MLA Handbook (7^th Edition):

Stumpf, Miriam. “Interplay of PTEN subcellular localization and catalytic activities in vivo.” 2016. Web. 01 Mar 2021.

Vancouver:

Stumpf M. Interplay of PTEN subcellular localization and catalytic activities in vivo. [Internet] [Doctoral dissertation]. Universiteit Utrecht; 2016. [cited 2021 Mar 01]. Available from: http://dspace.library.uu.nl:8080/handle/1874/334160.

Council of Science Editors:

Stumpf M. Interplay of PTEN subcellular localization and catalytic activities in vivo. [Doctoral Dissertation]. Universiteit Utrecht; 2016. Available from: http://dspace.library.uu.nl:8080/handle/1874/334160

NSYSU

25. Song , Yi-Chun. Coral-derived natural marine compound GB9 inhibits vascular development in zebrafish.

Degree: Master, Biological Sciences, 2017, NSYSU

URL: http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0716117-115842

► Vascular development is important for vertebrates, and genetic control vascular patterning has been intensively characterized. In addition, many studies have been show environmental hormones and… (more)

▼ Vascular development is important for vertebrates, and genetic control vascular patterning has been intensively characterized. In addition, many studies have been show environmental hormones and chemical compounds affect vascular growth. Natural marine compound GB9 was isolated from the marine soft coral Capnella imbricate, the study show GB9 had anti-neuroinflammatory and anti-nociceptive effects. However, the effect of GB9 on blood vessels is still unknown. In this study, we use transgene zebrafish as an animal model to understand the effect of vascular development in GB9 treated embryo. The results showed that GB9 treated embryos impair ISV (intersegmental vessel) growth, and CVP (caudal vein plexus) sprouting, which leads to pericardial edema and circulation defects. TUNEL assay and AO staining showed that vascular defects were not caused by apoptosis, but likely due to the impairment of cell proliferation and/or migration. We further showed GB9 treatment reduce cell proliferation marker p-HH3 and protein level, by counting p-HH3 immunostaining cell and Western blotting. In addition, we quantitated the migration distance of ISV between 24hpf and 28hpf and perform wound healing assay in endothelial cells EA.hy926. We show that GB9 treatment inhibits cell migration. Next, we examined the molecular mechanism of vascular defects by in situ hybridization, qPCR and Western blot, the results showed GB9 treatment decreases the expression of arteriovenous markers, ephrinb2, flt4, mrc1, flk and stabilinï¼the protein levels related to VEGF, BMP signal pathways. Those results suggest that GB9 interfere vascular signaling regulation in zebrafish. Besides, we examine whether GB9 treatment cause vascular defect due to the increase of oxidative stress? We co-treated low-dose GB9 and H2O2, observed that defects in CVP formation was more severe than single treatment. Moreover, antioxidants Acetyl-cysteine (NAC) can rescue vascular defects in GB9 treated embryos. These data suggested that GB9 exposure causes vascular defects mediated by an increase in oxidative. Together our data show that GB9 treatment affect vascular development mediated by the interference of VEGF and BMP signals by the enhance of oxidative stress. Advisors/Committee Members: Tzu-Fun Fu (chair), Zhi-Hong Wen (chair), Yung-Jen Chuang (chair), Chang-Yi Wu (committee member), ming-hong Tai (chair).

Subjects/Keywords: zebrafish; marine compound; angiogenesis; GB9

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APA (6^th Edition):

Song , Y. (2017). Coral-derived natural marine compound GB9 inhibits vascular development in zebrafish. (Thesis). NSYSU. Retrieved from http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0716117-115842

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Song , Yi-Chun. “Coral-derived natural marine compound GB9 inhibits vascular development in zebrafish.” 2017. Thesis, NSYSU. Accessed March 01, 2021. http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0716117-115842.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Song , Yi-Chun. “Coral-derived natural marine compound GB9 inhibits vascular development in zebrafish.” 2017. Web. 01 Mar 2021.

Vancouver:

Song Y. Coral-derived natural marine compound GB9 inhibits vascular development in zebrafish. [Internet] [Thesis]. NSYSU; 2017. [cited 2021 Mar 01]. Available from: http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0716117-115842.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Song Y. Coral-derived natural marine compound GB9 inhibits vascular development in zebrafish. [Thesis]. NSYSU; 2017. Available from: http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0716117-115842

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

NSYSU

26. Tu, Chia-Wen. Effects of exercise hormone irisin in glucocorticoid-induced osteoporosis.

Degree: Master, Biological Sciences, 2017, NSYSU

URL: http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0723117-220830

► Regular exercise stimulated the expression of the Fibronectin type III domain-containing protein 5 (FNDC5) in skeletal muscle. In post-translation modification process, FNDC5 cleavage to a… (more)

Subjects/Keywords: GIOP; IrisinR75E; Irisin; Zebrafish

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Tu, C. (2017). Effects of exercise hormone irisin in glucocorticoid-induced osteoporosis. (Thesis). NSYSU. Retrieved from http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0723117-220830

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Tu, Chia-Wen. “Effects of exercise hormone irisin in glucocorticoid-induced osteoporosis.” 2017. Thesis, NSYSU. Accessed March 01, 2021. http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0723117-220830.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Tu, Chia-Wen. “Effects of exercise hormone irisin in glucocorticoid-induced osteoporosis.” 2017. Web. 01 Mar 2021.

Vancouver:

Tu C. Effects of exercise hormone irisin in glucocorticoid-induced osteoporosis. [Internet] [Thesis]. NSYSU; 2017. [cited 2021 Mar 01]. Available from: http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0723117-220830.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Tu C. Effects of exercise hormone irisin in glucocorticoid-induced osteoporosis. [Thesis]. NSYSU; 2017. Available from: http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0723117-220830

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

27. Bournele, Despina. Μελέτη των μηχανισμών εκδήλωσης συγγενών καρδιοπαθειών με την χρήση του zebrafish ως πειραματικό μοντέλο.

Degree: 2016, National and Kapodistrian University of Athens; Εθνικό και Καποδιστριακό Πανεπιστήμιο Αθηνών (ΕΚΠΑ)

URL: http://hdl.handle.net/10442/hedi/38345

► Cardiovascular disease (CVD) is a group of disorders of the heart and blood vessels and one of the leading causes of death worldwide. The most… (more)

▼ Cardiovascular disease (CVD) is a group of disorders of the heart and blood vessels and one of the leading causes of death worldwide. The most significant risk factors associated with the development of heart diseases include genetic and environmental factors such as hypertension, high blood cholesterol levels, diabetes, smoking, and obesity. Coronary artery disease and stroke account for the highest percentage of CVD deaths and stroke, cardiomyopathies, congenital heart diseases, heart valve defects and arrhythmias follow. The causes, prevention, and treatment of all forms of cardiovascular disease remain active fields of biomedical research, with hundreds of scientific studies published on a weekly basis.Cardiac valves develop as the heart contracts, and they function throughout the lifetime of the organism to prevent retrograde blood flow. Their precise morphogenesis is crucial for cardiac function and depends on the interaction between cardiac contractility and intracardiac flow dynamics. Multiple signaling pathways are implicated in the morphogenesis of cardiac valves but little is known about the molecular mechanisms that orchestrate their formation.Several unique characteristics make zebrafish an attractive model for elucidating the mechanisms underlying cardiac valve morphogeneis. Non-invasive imaging allows in vivo analyses of cardiovascular phenotypes. In addition, zebrafish hearts maintain their ability to regenerate throughout their lifetime, providing novel insights to understand human cardiac regeneration. Zebrafish has also emerged as a high-throughput but low-cost model organism that combines the advantages of forward and reverse genetics with phenotype-driven drug screenings. Functional verification of candidate genes from Genome Wide Association Studies (GWAS) has verified the role of several genes in the pathophysiology of CVDs. Zebrafish is a valuable tool for the identification of candidate genes for congenital heart defects, either through mutant characterization from genetic screens or functional annotation through morphant analysis.Here we describe the identification and the characterization of genes involved in cardiac valve formation. The mutant lines that were used have derived from a forward mutagenesis genetic screen that was carried out in order to identify mutant phenotypes relevant to various aspects of cardiac development. Bua mutant embryos show an outflow tract stenosis phenotype, which results to abnormal atrioventricular valve formation and retrograde blood flow. BH mutants have smaller heart, body axis and head compared to wild-type embryos and have also curved tail.In parallel, we carried out the characterization of a new transgenic line for the wnt/b-catenin signaling pathway. Apc mutant embryos which show constitutive active wnt/b-catenin signaling have also been used for the elaborate characterization of the Tg(7xTCF-Xla.Siam:nlsmCherry) transgenic line.The goal of this research project was the study of gene function, the identification of new signaling pathways and the…

Subjects/Keywords: Συγγενείς καρδιοπάθειες; Cardiovascular disease; Zebrafish

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Bournele, D. (2016). Μελέτη των μηχανισμών εκδήλωσης συγγενών καρδιοπαθειών με την χρήση του zebrafish ως πειραματικό μοντέλο. (Thesis). National and Kapodistrian University of Athens; Εθνικό και Καποδιστριακό Πανεπιστήμιο Αθηνών (ΕΚΠΑ). Retrieved from http://hdl.handle.net/10442/hedi/38345

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Bournele, Despina. “Μελέτη των μηχανισμών εκδήλωσης συγγενών καρδιοπαθειών με την χρήση του zebrafish ως πειραματικό μοντέλο.” 2016. Thesis, National and Kapodistrian University of Athens; Εθνικό και Καποδιστριακό Πανεπιστήμιο Αθηνών (ΕΚΠΑ). Accessed March 01, 2021. http://hdl.handle.net/10442/hedi/38345.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Bournele, Despina. “Μελέτη των μηχανισμών εκδήλωσης συγγενών καρδιοπαθειών με την χρήση του zebrafish ως πειραματικό μοντέλο.” 2016. Web. 01 Mar 2021.

Vancouver:

Bournele D. Μελέτη των μηχανισμών εκδήλωσης συγγενών καρδιοπαθειών με την χρήση του zebrafish ως πειραματικό μοντέλο. [Internet] [Thesis]. National and Kapodistrian University of Athens; Εθνικό και Καποδιστριακό Πανεπιστήμιο Αθηνών (ΕΚΠΑ); 2016. [cited 2021 Mar 01]. Available from: http://hdl.handle.net/10442/hedi/38345.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Bournele D. Μελέτη των μηχανισμών εκδήλωσης συγγενών καρδιοπαθειών με την χρήση του zebrafish ως πειραματικό μοντέλο. [Thesis]. National and Kapodistrian University of Athens; Εθνικό και Καποδιστριακό Πανεπιστήμιο Αθηνών (ΕΚΠΑ); 2016. Available from: http://hdl.handle.net/10442/hedi/38345

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Manchester

28. Haud, Noemie Magali Renee. A forward genetic screen to identify modifiers of chemotherapy using zebrafish- Study of rnaset2 deficiency in zebrafish.

Degree: 2010, University of Manchester

URL: http://www.manchester.ac.uk/escholar/uk-ac-man-scw:90343

► Chemotherapy frequently fails to cure cancer patients due to toxicity or resistance to treatment. Variability in toxicity and resistance is influenced by polymorphisms and mutations… (more)

▼ Chemotherapy frequently fails to cure cancer patients due to toxicity or resistance to treatment. Variability in toxicity and resistance is influenced by polymorphisms and mutations in the individual's genome (Pharmacogenetics). Zebrafish has extensive evolutionary conservation with human, its genetics is a powerful gene discovery tool, and it has been described as a suitable model in cancer research. To study chemotherapy resistance, we used ENU-mutagenised zebrafish in a forward genetic screen to identify genes that modify responses to cancer chemotherapy. Zebrafish larvae were challenged with two chemotherapeutic drugs and stained with acridine orange (AO) to detect apoptosis and reveal hypo- or hyper-responders to chemotherapy. A mutation, conferring an increased uptake of AO, was identified by genetic mapping as a premature stop codon truncating the ribonuclease T2 (rnaset2) gene. Human RNASET2 encodes a putative lysosomal RNase. Lysosomal storage disorders, due to deficiencies in lysosomal hydrolases and resultant accumulation of macromolecules within lysosomes, are collectively among the commonest genetic diseases. RNASET2 deficiency in man results in a static encephalopathy arising in infancy and characterized by multifocal bilateral white matter lesions, subcortical cysts and focal enlargement of the anterior inferior horn. This doctoral thesis demonstrates that rnaset2 deficient zebrafish embryos suffer from a lysosomal storage disorder accumulating undigested ribosomal RNA (rRNA) in enlarged lysosomes within neurons of the brain. Moreover, high-field intensity μMRI revealed white matter lesions in the brain of adult rnase2 mutant animals. Thus, this zebrafish mutant can be used as a model to study the abnormalities observed in RNASET2 deficient individuals. This model suggests that the leukoencephalopathy results from a lysosomal storage disorder and provides a preclinical model for further elaborating disease mechanisms and evaluating candidate therapeutics.RNASET2 has also been advanced as a candidate tumour suppressor in several solid tumours. Recombinant rnaset2 protein has been tested in the clinic as an anti-cancer cytotoxic agent, with anti-angiogenic properties. By combining the rnaset2 mutant presented here with a transgenic melanoma model developed in the laboratory, the tumour suppressor and angiogenic role for rnaset2 was refuted. Advisors/Committee Members: Hurlstone, Adam.

Subjects/Keywords: lysosomes; zebrafish

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Haud, N. M. R. (2010). A forward genetic screen to identify modifiers of chemotherapy using zebrafish- Study of rnaset2 deficiency in zebrafish. (Doctoral Dissertation). University of Manchester. Retrieved from http://www.manchester.ac.uk/escholar/uk-ac-man-scw:90343

Chicago Manual of Style (16^th Edition):

Haud, Noemie Magali Renee. “A forward genetic screen to identify modifiers of chemotherapy using zebrafish- Study of rnaset2 deficiency in zebrafish.” 2010. Doctoral Dissertation, University of Manchester. Accessed March 01, 2021. http://www.manchester.ac.uk/escholar/uk-ac-man-scw:90343.

MLA Handbook (7^th Edition):

Haud, Noemie Magali Renee. “A forward genetic screen to identify modifiers of chemotherapy using zebrafish- Study of rnaset2 deficiency in zebrafish.” 2010. Web. 01 Mar 2021.

Vancouver:

Haud NMR. A forward genetic screen to identify modifiers of chemotherapy using zebrafish- Study of rnaset2 deficiency in zebrafish. [Internet] [Doctoral dissertation]. University of Manchester; 2010. [cited 2021 Mar 01]. Available from: http://www.manchester.ac.uk/escholar/uk-ac-man-scw:90343.

Council of Science Editors:

Haud NMR. A forward genetic screen to identify modifiers of chemotherapy using zebrafish- Study of rnaset2 deficiency in zebrafish. [Doctoral Dissertation]. University of Manchester; 2010. Available from: http://www.manchester.ac.uk/escholar/uk-ac-man-scw:90343

Vanderbilt University

29. Li, Nan. MiRNA function during early vertebrate development.

Degree: PhD, Biological Sciences, 2010, Vanderbilt University

URL: http://hdl.handle.net/1803/12868

► microRNAs (miRNAs) are short non-coding RNAs that down-regulate gene expression by pairing with sequences in the 3’UTR of target mRNAs. They play critical roles in… (more)

Subjects/Keywords: zebrafish; microRNA

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Li, N. (2010). MiRNA function during early vertebrate development. (Doctoral Dissertation). Vanderbilt University. Retrieved from http://hdl.handle.net/1803/12868

Chicago Manual of Style (16^th Edition):

Li, Nan. “MiRNA function during early vertebrate development.” 2010. Doctoral Dissertation, Vanderbilt University. Accessed March 01, 2021. http://hdl.handle.net/1803/12868.

MLA Handbook (7^th Edition):

Li, Nan. “MiRNA function during early vertebrate development.” 2010. Web. 01 Mar 2021.

Vancouver:

Li N. MiRNA function during early vertebrate development. [Internet] [Doctoral dissertation]. Vanderbilt University; 2010. [cited 2021 Mar 01]. Available from: http://hdl.handle.net/1803/12868.

Council of Science Editors:

Li N. MiRNA function during early vertebrate development. [Doctoral Dissertation]. Vanderbilt University; 2010. Available from: http://hdl.handle.net/1803/12868

Vanderbilt University

30. Carlin, Daniel L. The homeodomain transcription factor Six3 is required for telencephalon patterning in zebrafish.

Degree: PhD, Biological Sciences, 2012, Vanderbilt University

URL: http://hdl.handle.net/1803/14113

► The adult vertebrate forebrain is responsible for a diverse set of behaviors, and as such exhibits complex anatomy. This complexity is generated during embryogenesis whereby… (more)

▼ The adult vertebrate forebrain is responsible for a diverse set of behaviors, and as such exhibits complex anatomy. This complexity is generated during embryogenesis whereby a specific spatiotemporal sequence of transcriptional and signaling programs promotes specification of different cell types based on the location and developmental potential of progenitors. Six3 is one such transcription factor that exerts multiple functions in the development of anterior neural tissue of vertebrate embryos. Whereas complete loss of Six3 function in the mouse results in failure of forebrain formation, its hypomorphic mutations in human and mouse can promote holoprosencephaly, a forebrain malformation resulting, at least in part, from abnormal telencephalon development. However, Six3’s roles in telencephalon patterning and differentiation are not well understood. The zebrafish genome contains three Six3-related genes facilitating analysis of different partial loss-of-function combinations. I analyzed zebrafish embryos deficient in two of three Six3-related genes, six3b and six7, representing a partial loss of Six3 function. Telencephalon forms in six3b;six7-deficient embryos, however ventral telencephalic domains are reduced and dorsal domains are expanded. Decreased cell proliferation or excess apoptosis cannot account for the ventral deficiency. Instead, six3b and six7 are required during early segmentation for specification of ventral progenitors, similar to the role of Hedgehog signaling in telencephalon development. Unlike in mice, Hedgehog signaling is not disrupted in embryos with reduced Six3 function. Furthermore, six3b overexpression is sufficient to compensate for loss of Hedgehog signaling in isl1- but not nkx2.1b-positive cells, suggesting a novel Hedgehog-independent role for Six3 in telencephalon patterning. Additional investigations into the interactions between Six3 and known telencepnalon patterning genes showed that Six3 promotes ventral telencephalic fates through transient regulation of foxg1a expression and repression of Wnt/β-catenin pathway. As Six3-related genes are expressed broadly in prechordal mesoderm and anterior neuroectoderm, transgenic zebrafish were generated to identify the spatial requirement for Six3 function in telencephalon patterning. My studies help define the cellular mechanisms of Six3-mediated dorsoventral patterning in telencephalon and present a novel genetic mechanism by which Six3 regulates this process. Advisors/Committee Members: Joshua Gamse (committee member), Kenneth Catania (committee member), Chin Chiang (committee member), Lilianna Solnica-Krezel (committee member), Laurence Zwiebel (Committee Chair).

Subjects/Keywords: telencephalon; Foxg1; Six3; zebrafish

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Carlin, D. L. (2012). The homeodomain transcription factor Six3 is required for telencephalon patterning in zebrafish. (Doctoral Dissertation). Vanderbilt University. Retrieved from http://hdl.handle.net/1803/14113

Chicago Manual of Style (16^th Edition):

Carlin, Daniel L. “The homeodomain transcription factor Six3 is required for telencephalon patterning in zebrafish.” 2012. Doctoral Dissertation, Vanderbilt University. Accessed March 01, 2021. http://hdl.handle.net/1803/14113.

MLA Handbook (7^th Edition):

Carlin, Daniel L. “The homeodomain transcription factor Six3 is required for telencephalon patterning in zebrafish.” 2012. Web. 01 Mar 2021.

Vancouver:

Carlin DL. The homeodomain transcription factor Six3 is required for telencephalon patterning in zebrafish. [Internet] [Doctoral dissertation]. Vanderbilt University; 2012. [cited 2021 Mar 01]. Available from: http://hdl.handle.net/1803/14113.

Council of Science Editors:

Carlin DL. The homeodomain transcription factor Six3 is required for telencephalon patterning in zebrafish. [Doctoral Dissertation]. Vanderbilt University; 2012. Available from: http://hdl.handle.net/1803/14113

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