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1.
Thorn, Robert Joseph.
Development, Disease, and Regeneration: Using Zebrafish to
Model Neurological Perturbations.
Degree: Department of Molecular Biology, Cell Biology and
Biochemistry, 2018, Brown University
URL: https://repository.library.brown.edu/studio/item/bdr:792820/
► As medical technologies advance, effective vertebrate models of human disease are vital to determine safety and efficacy of treatments. My research has further developed zebrafish…
(more)
▼ As medical technologies advance, effective vertebrate
models of human disease are vital to determine safety and efficacy
of treatments. My research has further developed
zebrafish as a
model to investigate vertebrate neurological development and
disease.
Zebrafish are well suited for use as a model of vertebrate
disease. A mixed population of male and female fish can produce
hundreds of embryos on a daily basis. The embryos are transparent,
develop externally, and can be imaged live by light microscopy
throughout development. They are accessible to genetic manipulation
and have many genes that are homologous to human disease genes.
Zebrafish larvae display robust stereotyped behaviors that can be
assayed to detect subtle brain defects. In my thesis work, I used
zebrafish to model developmental sensitivities to immunosuppressant
drugs that may be prescribed during human fetal development. My
research indicated that these drugs have a negative effect on brain
and behavioral development. I’ve shown that
zebrafish are also
useful in testing neurodevelopmental toxicity of small molecules.
Additionally, I have used morpholinos in
zebrafish to knock down
calcineurin, whose decreased signaling has been implicated in Down
syndrome disease phenotypes. My research displayed developmental
brain defects and behavioral defects in the calcineurin morphants,
potentially due to increased apoptosis early in development. This
model can also be utilized to test pharmaceuticals that may treat
Down syndrome phenotypes. Finally, I used
zebrafish as a
high-throughput model for the loss and recovery of vertebrate
vision. My work showed that
zebrafish behavior can be used as a
method to detect loss and recovery of larval
zebrafish vision in
multi-well plates. This model will be useful in testing compounds
to treat human visual diseases, especially those that may be used
in conjunction with promising stem cell therapies. Overall my
research has made strides in using
zebrafish as a vertebrate model
of neurodevelopmental aberrations and potential therapeutic
screening of pharmaceutical compounds.
Advisors/Committee Members: Hart, Anne (Reader), Wessel, Gary (Reader), Creton, Robbert (Advisor), Mowry, Kimberly (Reader), DeLong, Alison (Reader), Aluru, Neel (Reader).
Subjects/Keywords: Zebrafish
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APA ·
Chicago ·
MLA ·
Vancouver ·
CSE |
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APA (6th Edition):
Thorn, R. J. (2018). Development, Disease, and Regeneration: Using Zebrafish to
Model Neurological Perturbations. (Thesis). Brown University. Retrieved from https://repository.library.brown.edu/studio/item/bdr:792820/
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Thorn, Robert Joseph. “Development, Disease, and Regeneration: Using Zebrafish to
Model Neurological Perturbations.” 2018. Thesis, Brown University. Accessed March 01, 2021.
https://repository.library.brown.edu/studio/item/bdr:792820/.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Thorn, Robert Joseph. “Development, Disease, and Regeneration: Using Zebrafish to
Model Neurological Perturbations.” 2018. Web. 01 Mar 2021.
Vancouver:
Thorn RJ. Development, Disease, and Regeneration: Using Zebrafish to
Model Neurological Perturbations. [Internet] [Thesis]. Brown University; 2018. [cited 2021 Mar 01].
Available from: https://repository.library.brown.edu/studio/item/bdr:792820/.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Thorn RJ. Development, Disease, and Regeneration: Using Zebrafish to
Model Neurological Perturbations. [Thesis]. Brown University; 2018. Available from: https://repository.library.brown.edu/studio/item/bdr:792820/
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
2.
Ojo, Oladele Temitope Adedayo.
Screen for a Novel Pharmaceutical that Stimulates
Photoreceptor Regeneration in Zebrafish Utilizing a Behavioral
Assay.
Degree: Department of Molecular Biology, Cell Biology and
Biochemistry, 2017, Brown University
URL: https://repository.library.brown.edu/studio/item/bdr:733461/
► Zebrafish have demonstrated a remarkable ability to regenerate following damage to their visual system. However, little is known about the underlying mechanisms for this regeneration,…
(more)
▼ Zebrafish have demonstrated a remarkable ability to
regenerate following damage to their visual system. However, little
is known about the underlying mechanisms for this regeneration,
which may be exploited to stimulate regeneration of the visual
system in
zebrafish and other organisms. In this study, we
developed a behavioral assay for measuring the loss and recovery of
vision. In addition, we examined the effect of cyclosporine, a
calcineurin inhibitor, on the recovery of vision because of its
established neuroprotective effects. Here we show that cyclosporine
stimulates the recovery of vision in
zebrafish larvae. We found
that exposure to 20uM solution of cyclosporine for as little as 6
hours, caused robust recovery in visual capabilities following 330
seconds of UV illumination compared to those not exposed to
cyclosporine. This shows that the recovery previously observed in
zebrafish can be stimulated and indicates a possible mechanism of
recovery. This study shows that high throughput behavioral assays
can be used to examine the loss and recovery of vertebrate vision
and provide an efficient platform for the discovery of novel
pharmaceuticals that stimulate regeneration of the visual
system.
Advisors/Committee Members: Morgan, Jeffery (Reader), Aizenman, Carlos (Reader), Créton, Robbert (Advisor).
Subjects/Keywords: Zebrafish
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❌
APA ·
Chicago ·
MLA ·
Vancouver ·
CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Ojo, O. T. A. (2017). Screen for a Novel Pharmaceutical that Stimulates
Photoreceptor Regeneration in Zebrafish Utilizing a Behavioral
Assay. (Thesis). Brown University. Retrieved from https://repository.library.brown.edu/studio/item/bdr:733461/
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Ojo, Oladele Temitope Adedayo. “Screen for a Novel Pharmaceutical that Stimulates
Photoreceptor Regeneration in Zebrafish Utilizing a Behavioral
Assay.” 2017. Thesis, Brown University. Accessed March 01, 2021.
https://repository.library.brown.edu/studio/item/bdr:733461/.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Ojo, Oladele Temitope Adedayo. “Screen for a Novel Pharmaceutical that Stimulates
Photoreceptor Regeneration in Zebrafish Utilizing a Behavioral
Assay.” 2017. Web. 01 Mar 2021.
Vancouver:
Ojo OTA. Screen for a Novel Pharmaceutical that Stimulates
Photoreceptor Regeneration in Zebrafish Utilizing a Behavioral
Assay. [Internet] [Thesis]. Brown University; 2017. [cited 2021 Mar 01].
Available from: https://repository.library.brown.edu/studio/item/bdr:733461/.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Ojo OTA. Screen for a Novel Pharmaceutical that Stimulates
Photoreceptor Regeneration in Zebrafish Utilizing a Behavioral
Assay. [Thesis]. Brown University; 2017. Available from: https://repository.library.brown.edu/studio/item/bdr:733461/
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Oregon State University
3.
Norris, Lauren J.
Controlling Important Pathogens in Zebrafish (Danio rerio): Assessing Cryopreservation Survival of Bacteria and Parasites and Clinical Sensitivity of Mycobacterial qPCR Assays.
Degree: MS, 2017, Oregon State University
URL: http://hdl.handle.net/1957/61867
► Zebrafish (Danio rerio) are one of the most commonly used animal models in biomedical research. Zebrafish resource facilities, like the Zebrafish International Resource Center (ZIRC)…
(more)
▼ Zebrafish (Danio rerio) are one of the most commonly used animal models in biomedical research.
Zebrafish resource facilities, like the
Zebrafish International Resource Center (ZIRC) in Eugene, Oregon, are the main providers and keepers of numerous
zebrafish wild-type, mutant, and transgenic lines. Although ZIRC maintains live
zebrafish at various life stages, sperm cryopreservation allows them to maintain the vast array of
zebrafish lines that they receive from outside facilities. Hence, there is a concern about the potential of vertical transmission of pathogens capable of surviving the freezing and thawing process.
My first study was to determine whether
zebrafish pathogens are capable of surviving the sperm cryopreservation process used by ZIRC (i.e., the ZIRC method). I assessed the survival of two strains of Mycobacterium chelonae (H1E1 and H1E2), one strain of Mycobacterium marinum (OSU 214), one strain of Edwardsiella ictaluri, Pseudocapillaria tomentosa eggs and Pseudoloma neurophilia spores, which are all
pathogens of concern in
zebrafish research facilities. These pathogens were also frozen and thawed without cryopreservant, and the pathogens were frozen at either -80 °C or - 20 °C with only a small amount of Phosphate Buffered Saline (PBS).
Each bacterial species survived both freezing and thawing methods, however the samples subjected to the ZIRC method had the higher percentages of bacterial survival compared to the freezing without cryopreservant samples. The mycobacteria had higher survival rates compared to Gram-negative E. ictaluri in both freezing methods. E. ictaluri exhibited a 1-2 log decrease in concentration following the freezing without cryopreservant.
For the P. tomentosa eggs, survival was based on larvation. Eggs were examined at Day 0 (immediately after collecting or thawing) and at Day 7 (a week after being collected or thawed). No larvation was observed on Day 7 with eggs processed by the ZIRC method or simple freezing (-80 °C in 1X PBS and no cryopreservant). In contrast, the positive controls, kept at 28 °C, showed 80-93% larvation at Day 7. Most of the eggs observed in either freezing method were unlarvated and intact, however some, exhibited signs of internal deformation of the egg contents.
In 2014, our lab conducted a similar cryopreservation study on the ZIRC cryopreservation method in place at that time. In that study P. neurophilia spores were tested for their ability to survive cryopreservation. I repeated this study in 2017 using the 2017 ZIRC cryopreservation protocol. In both experiments, two fluorescent stains, SYTOX and Fungi-Fluor, and presence of a spore vacuole were used to determine spore viability. SYTOX green is a fluorescent nucleic acid stain, and cells are scored as dead when the dye enters the cells and results in green fluorescence. P. neurophilia spores also
contain a long, coiled, polar filament or tube that is thought to aid in infecting hosts cells when extruded. Spores are scored as alive if they are stained with Fungi-Fluor, exposed to ultra violet…
Advisors/Committee Members: Kent, Michael L. (advisor), Halsey, Kimberly H. (committee member).
Subjects/Keywords: Zebrafish
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❌
APA ·
Chicago ·
MLA ·
Vancouver ·
CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Norris, L. J. (2017). Controlling Important Pathogens in Zebrafish (Danio rerio): Assessing Cryopreservation Survival of Bacteria and Parasites and Clinical Sensitivity of Mycobacterial qPCR Assays. (Masters Thesis). Oregon State University. Retrieved from http://hdl.handle.net/1957/61867
Chicago Manual of Style (16th Edition):
Norris, Lauren J. “Controlling Important Pathogens in Zebrafish (Danio rerio): Assessing Cryopreservation Survival of Bacteria and Parasites and Clinical Sensitivity of Mycobacterial qPCR Assays.” 2017. Masters Thesis, Oregon State University. Accessed March 01, 2021.
http://hdl.handle.net/1957/61867.
MLA Handbook (7th Edition):
Norris, Lauren J. “Controlling Important Pathogens in Zebrafish (Danio rerio): Assessing Cryopreservation Survival of Bacteria and Parasites and Clinical Sensitivity of Mycobacterial qPCR Assays.” 2017. Web. 01 Mar 2021.
Vancouver:
Norris LJ. Controlling Important Pathogens in Zebrafish (Danio rerio): Assessing Cryopreservation Survival of Bacteria and Parasites and Clinical Sensitivity of Mycobacterial qPCR Assays. [Internet] [Masters thesis]. Oregon State University; 2017. [cited 2021 Mar 01].
Available from: http://hdl.handle.net/1957/61867.
Council of Science Editors:
Norris LJ. Controlling Important Pathogens in Zebrafish (Danio rerio): Assessing Cryopreservation Survival of Bacteria and Parasites and Clinical Sensitivity of Mycobacterial qPCR Assays. [Masters Thesis]. Oregon State University; 2017. Available from: http://hdl.handle.net/1957/61867
4.
Sarantis, Panagiotis.
Μελέτη μηχανισμών καρδιοπαθειών χρησιμοποιώντας το Zebrafish ως πειραματικό μοντέλο.
Degree: 2020, National and Kapodistrian University of Athens; Εθνικό και Καποδιστριακό Πανεπιστήμιο Αθηνών (ΕΚΠΑ)
URL: http://hdl.handle.net/10442/hedi/47888
► Cardiovascular diseases are nowadays one of the leading causes of death in the developed world. Also very important is the contribution of angiogenesis to the…
(more)
▼ Cardiovascular diseases are nowadays one of the leading causes of death in the developed world. Also very important is the contribution of angiogenesis to the development of cancerous tumors. It is therefore necessary to contribute to the basic research in order to better study the mechanisms of these diseases but also to discover new drugs related to angiogenesis. In recent years, the zebrafish animal model has played an important role in these conditions. Zebrafish (Danio rerio) is widely used as an animal model to understand the pathophysiology of cardiovascular diseases. Here, we present the adult cardiac phenotype of weak atrium, myh6-/-, which carry mutations in the zebrafish atrial myosin heavy chain. Homozygous mutants survive to adulthood and are fertile despite their initial weak atrial beat. In adult mutants, the atrium remains hypoplastic and shows elastin deposition while mutant ventricles exhibit increased size. In mammals, hypertrophy is the most common mechanism resulting in cardiomegaly. Using immunohistochemistry and confocal microscopy to measure cardiomyocyte cell size, density and proliferation, we show that the enlargement of the myh6-/- ventricle is predominantly due to hyperplasia. However, we identified similar transcriptional profiles to the mammalian hypertrophy response via RT-PCR of the hyperplastic ventricles. Furthermore, we show activation of the ER-stress pathway by western blot analysis. In conclusion, we can assume, based on our model, that molecular signaling pathways associated with hypertrophy in mammals, in combination with ER-stress activation, result in hyperplasia in zebrafish. In addition, to our knowledge, this is the first time to report elastin deposition in the atrium. In parallel, we studied small molecules that inhibit angiogenesis and are attractive drug candidates for cancer, retinopathies, and age-related macular degeneration. In vivo phenotypic screening in zebrafish (Danio rerio) emerges as a powerful methodology to identify and optimize novel compounds with pharmacological activity. Zebrafish provides several advantages for in vivo phenotypic screens especially for angiogenesis, since it develops rapidly, externally, and does not rely on a functional cardiovascular system to survive for several days during development. In this study, we utilize a transgenic line that allows the noninvasive monitoring of angiogenesis at a cellular level. The inhibition of angiogenesis can be observed under a fluorescent stereoscope and quantified. To exemplify the versatility and robustness of the zebrafish screen, we have employed a series of 60 novel compounds that were designed based on a potent VEGFR2 inhibitor. Herein, we report their structure-based design, synthesis, and in vivo zebrafish screening for optimal activity, toxicity, and off-target effects, which revealed six reversible inhibitors of angiogenesis. Finally, in the present doctoral dissertation, genes were attempted to play a role in the morphogenesis of the heart valves and to demonstrate their relationship…
Subjects/Keywords: Kαρδιά; Zebrafish; Heart; Zebrafish
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❌
APA ·
Chicago ·
MLA ·
Vancouver ·
CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Sarantis, P. (2020). Μελέτη μηχανισμών καρδιοπαθειών χρησιμοποιώντας το Zebrafish ως πειραματικό μοντέλο. (Thesis). National and Kapodistrian University of Athens; Εθνικό και Καποδιστριακό Πανεπιστήμιο Αθηνών (ΕΚΠΑ). Retrieved from http://hdl.handle.net/10442/hedi/47888
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Sarantis, Panagiotis. “Μελέτη μηχανισμών καρδιοπαθειών χρησιμοποιώντας το Zebrafish ως πειραματικό μοντέλο.” 2020. Thesis, National and Kapodistrian University of Athens; Εθνικό και Καποδιστριακό Πανεπιστήμιο Αθηνών (ΕΚΠΑ). Accessed March 01, 2021.
http://hdl.handle.net/10442/hedi/47888.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Sarantis, Panagiotis. “Μελέτη μηχανισμών καρδιοπαθειών χρησιμοποιώντας το Zebrafish ως πειραματικό μοντέλο.” 2020. Web. 01 Mar 2021.
Vancouver:
Sarantis P. Μελέτη μηχανισμών καρδιοπαθειών χρησιμοποιώντας το Zebrafish ως πειραματικό μοντέλο. [Internet] [Thesis]. National and Kapodistrian University of Athens; Εθνικό και Καποδιστριακό Πανεπιστήμιο Αθηνών (ΕΚΠΑ); 2020. [cited 2021 Mar 01].
Available from: http://hdl.handle.net/10442/hedi/47888.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Sarantis P. Μελέτη μηχανισμών καρδιοπαθειών χρησιμοποιώντας το Zebrafish ως πειραματικό μοντέλο. [Thesis]. National and Kapodistrian University of Athens; Εθνικό και Καποδιστριακό Πανεπιστήμιο Αθηνών (ΕΚΠΑ); 2020. Available from: http://hdl.handle.net/10442/hedi/47888
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Waikato
5.
Ahmadi, Ramtin.
Eating behaviour regulation in Zebrafish (Danio rerio): a comparative analysis
.
Degree: 2014, University of Waikato
URL: http://hdl.handle.net/10289/8791
► The structure, function, and tissue specific expression profiles of neuropeptides involved in regulating eating behaviour are well researched in mammals, the corresponding literature on fish…
(more)
▼ The structure, function, and tissue specific expression profiles of neuropeptides involved in regulating eating behaviour are well researched in mammals, the corresponding literature on fish homologs however is scarce. The work presented here endeavoured to characterise the full coding sequences of peptide homologs in
zebrafish as well as the expression profiles of said peptides under different energy states to elucidate their function in fish.
Zebrafish were chosen as a model to represent fish species as they are amenable to molecular study. Conducting a literature and TBLASTn search, genes were identified in
zebrafish that are known to be involved in affecting appetite and satiety in other vertebrates. Comparison of the translated sequences of these genes against fish and other vertebrates provided confidence in the identity of AGRP, GHRL, POMCb, CART1, CART3, and CART4 and all genes showed homology against mammalian transcripts through structural and syntenic comparison. RACE synthesis of the full coding sequence of these genes was attempted from isolated brain mRNA but only resulted in the production of a partial 3’ sequence for CART1 and CART4 which showed high identity against the predicted nucleotide but not the translated sequence. To observe the functional properties of a suite of genes, a qPCR analysis of the expression profiles of AGRP, GHRL, POMCa, POMCb, CART1, CART2, CART3, CART4, OXT, NPY, and AVPwas undertaken in 24 hour fasted
zebrafish. The results were comparable to both other fish and mammals as AGRP was orexigenically upregulated in fasted
zebrafish, while POMCb and OXT were anorexigenically downregulated. This is the first time that an expression study has been conducted across all of these genes together in a single species and, while the results are preliminary, they outline that the simultaneous analysis of the many genes thought to be involved in eating behaviour is a viable approach to discovering the functional roles of the range of genes, their interaction with each other, and how that relates to eating behaviour and provides some confidence in use of
zebrafish as a model to highlight this.
Advisors/Committee Members: Bird, Steve (advisor), Olszewski, Pawel K (advisor).
Subjects/Keywords: Zebrafish;
feeding
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❌
APA ·
Chicago ·
MLA ·
Vancouver ·
CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Ahmadi, R. (2014). Eating behaviour regulation in Zebrafish (Danio rerio): a comparative analysis
. (Masters Thesis). University of Waikato. Retrieved from http://hdl.handle.net/10289/8791
Chicago Manual of Style (16th Edition):
Ahmadi, Ramtin. “Eating behaviour regulation in Zebrafish (Danio rerio): a comparative analysis
.” 2014. Masters Thesis, University of Waikato. Accessed March 01, 2021.
http://hdl.handle.net/10289/8791.
MLA Handbook (7th Edition):
Ahmadi, Ramtin. “Eating behaviour regulation in Zebrafish (Danio rerio): a comparative analysis
.” 2014. Web. 01 Mar 2021.
Vancouver:
Ahmadi R. Eating behaviour regulation in Zebrafish (Danio rerio): a comparative analysis
. [Internet] [Masters thesis]. University of Waikato; 2014. [cited 2021 Mar 01].
Available from: http://hdl.handle.net/10289/8791.
Council of Science Editors:
Ahmadi R. Eating behaviour regulation in Zebrafish (Danio rerio): a comparative analysis
. [Masters Thesis]. University of Waikato; 2014. Available from: http://hdl.handle.net/10289/8791

University of Manchester
6.
Oltrabella, Francesca.
INVESTIGATION OF OCRL1 AND ITS INTERACTION PARTNERS IN
ZEBRAFISH.
Degree: 2014, University of Manchester
URL: http://www.manchester.ac.uk/escholar/uk-ac-man-scw:240811
► Oculocerebrorenal syndrome of Lowe is a rare X-linked disorder caused bymutation of the inositol 5-phosphatase OCRL1. Lowe Syndrome manifests as renaltubular dysfunction, neurological and ocular…
(more)
▼ Oculocerebrorenal syndrome of Lowe is a rare
X-linked disorder caused bymutation of the inositol 5-phosphatase
OCRL1. Lowe Syndrome manifests as renaltubular dysfunction,
neurological and ocular defects. OCRL1 uses its catalyticdomain to
hydrolyze two phosphoinositide species, PI(4,5)P2 and PI3,4,5)P3.
It isinvolved in regulation of membrane trafficking, actin
dynamics, cytokinesis andciliogenesis. OCRL1 interacts with IPIP27A
and B, which have been shown to bekey players in endocytic
trafficking in mammalian cells, specifically in the recyclingof
proteins from early and recycling endosomes to both the plasma
membrane andtrans-Golgi network. It has been proposed that
defective endocytic trafficking maybe responsible for the renal
tubulopathy seen in Lowe Syndrome patients,characterized by low
molecular weight proteinuria and aminoaciduria, but thishypothesis
has yet to be tested.Using zebrafish as a model for Lowe syndrome,
we show that depletion ofOcrl1 can indeed cause defects in
endocytosis in the renal tubule. This coincideswith a reduction in
levels of the multi-ligand receptor megalin, reduced abundanceof
the endocytic apparatus and increased numbers of enlarged lysosomes
in thekidney tubular cells. We also show that knocking-down Pip5K
in the Ocrl1 mutantsto rebalance PI(4,5)P2 levels can rescue the
endocytic defect. This indicates thattight control of PI(4,5)P2
level is essential for efficient endocytic trafficking in
vivo.Importantly, this finding suggests that Pip5K may be a
valuable therapeutic targetfor patients with Lowe Syndrome.To
further characterize the molecular mechanisms by which OCRL1
promotesendocytosis, we have focused on the recently identified
Ocrl1 interaction partnersIPIP27A and B, which are known to
function in endocytosis and receptor recycling.Here we report
identification and characterization of the zebrafish Ipip27s,
includinganalysis of conservation and expression profiles. To
assess Ipip27s function invivo, KO zebrafish lines were generated
using TALENs. This was successful forIpip27A, but so far not for
Ipip27B. Functional analysis using the Ipip27A KO lineand KD with
morpholinos revealed that both Ipip27s contribute to
neuraldevelopment and may participate in ciliogenesis. Moreover,
preliminary analysisindicates an important role for Ipip27A within
the endocytic pathway in the kidneytubule, where its loss
phenocopies many aspects of the Ocrl1 mutant
phenotype.
CD - MOVIE 1 AND MOVIE 2
Advisors/Committee Members: HURLSTONE, ADAM AFL, Hurlstone, Adam, Lowe, Martin.
Subjects/Keywords: OCRL1; ZEBRAFISH
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❌
APA ·
Chicago ·
MLA ·
Vancouver ·
CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Oltrabella, F. (2014). INVESTIGATION OF OCRL1 AND ITS INTERACTION PARTNERS IN
ZEBRAFISH. (Doctoral Dissertation). University of Manchester. Retrieved from http://www.manchester.ac.uk/escholar/uk-ac-man-scw:240811
Chicago Manual of Style (16th Edition):
Oltrabella, Francesca. “INVESTIGATION OF OCRL1 AND ITS INTERACTION PARTNERS IN
ZEBRAFISH.” 2014. Doctoral Dissertation, University of Manchester. Accessed March 01, 2021.
http://www.manchester.ac.uk/escholar/uk-ac-man-scw:240811.
MLA Handbook (7th Edition):
Oltrabella, Francesca. “INVESTIGATION OF OCRL1 AND ITS INTERACTION PARTNERS IN
ZEBRAFISH.” 2014. Web. 01 Mar 2021.
Vancouver:
Oltrabella F. INVESTIGATION OF OCRL1 AND ITS INTERACTION PARTNERS IN
ZEBRAFISH. [Internet] [Doctoral dissertation]. University of Manchester; 2014. [cited 2021 Mar 01].
Available from: http://www.manchester.ac.uk/escholar/uk-ac-man-scw:240811.
Council of Science Editors:
Oltrabella F. INVESTIGATION OF OCRL1 AND ITS INTERACTION PARTNERS IN
ZEBRAFISH. [Doctoral Dissertation]. University of Manchester; 2014. Available from: http://www.manchester.ac.uk/escholar/uk-ac-man-scw:240811

University of Adelaide
7.
Lim, Anne Hwee Ling.
Analysis of the subcellular localization of proteins implicated in Alzheimer’s disease.
Degree: 2015, University of Adelaide
URL: http://hdl.handle.net/2440/103503
► Alzheimer’s disease (AD) is a neurodegenerative disorder involving neuropathological changes including the presence of amyloid plaques in the brain. Amyloid plaques are comprised mainly of…
(more)
▼ Alzheimer’s disease (AD) is a neurodegenerative disorder involving neuropathological changes including the presence of amyloid plaques in the brain. Amyloid plaques are comprised mainly of the Aβ peptide, formed by the cleavage of the AMYLOID BETA A4 PRECURSOR PROTEIN (APP) by the enzyme complex γ-secretase. The catalytic component of the γ-secretase complex is PRESENILIN (PSEN1 or PSEN2). Mutations in the human PSEN1 gene have been identified in AD. Some mutations have been found to cause frame shifts leading to premature stop codons that produce transcripts encoding truncated PSEN1 protein. The subcellular location of the PSENs has been controversial, with studies detecting these proteins in multiple areas in the cell. However recent research has shown that the PSENs are enriched in the mitochondria associated membranes (MAM). The
zebrafish is a small freshwater fish that is a useful animal model for the study of human diseases.
Zebrafish have genes that are orthologous to human genes that play a role in AD, including those that encode the components of the γ-secretase complex. However, the
zebrafish orthologue of the NICASTRIN gene has not been studied in detail. In Chapter II, we characterize in
zebrafish the orthologue of the human NICASTRIN (NCSTN) gene that encodes a protein component of the γ-secretase. We demonstrate the spatial and temporal expression level of
zebrafish ncstn in embryos and various adult tissues. This work provides the basic knowledge required to further the understanding of the role of Ncstn in the γ-secretase complex using the
zebrafish animal model. Several truncations of the human PSEN1 and
zebrafish Psen1 proteins appear to have differential effects on Notch signalling and Appa cleavage. The basis for these differences is still unclear. Chapter III examines the subcellular localization of fusions of truncations of
zebrafish Psen1 protein with green fluorescent protein (GFP) using a simple protocol to obtain single cells from
zebrafish embryos for microscopy analysis. We show that the different truncations analysed appear to have similar distributions, suggesting that the differential effects on γ-secretase substrates are likely not due to different subcellular localisations of these truncated forms of Psen1. This chapter contributes a new method of viewing a single layer of
zebrafish cells with confocal microscopy. The mitochondria-associated membranes (MAM) are a subcompartment of the endoplasmic reticulum (ER) which interacts with mitochondria. The MAM is highly enriched in PSEN1 and PSEN2. Mitochondrion-ER appositions are increased in Psen1⁻ʹ⁻/Psen2⁻ʹ⁻ mouse embryonic fibroblasts (MEFs) and also in familial AD (FAD) and sporadic AD (SAD) patient cells. These results, which were performed using mammalian cells, have not been studied in the
zebrafish animal model. Therefore, Chapter IV of this thesis examines the effect of inhibiting
zebrafish psen1 and psen2 activity on mitochondrion-ER appositions in 24 hpf embryos. The findings in this study differ from those done in mammalian…
Advisors/Committee Members: Lardelli, Michael Trent (advisor), Kelly, Joan Maree (advisor), School of Biological Sciences (school).
Subjects/Keywords: Alzheimer’s; zebrafish
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APA ·
Chicago ·
MLA ·
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to Zotero / EndNote / Reference
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APA (6th Edition):
Lim, A. H. L. (2015). Analysis of the subcellular localization of proteins implicated in Alzheimer’s disease. (Thesis). University of Adelaide. Retrieved from http://hdl.handle.net/2440/103503
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Lim, Anne Hwee Ling. “Analysis of the subcellular localization of proteins implicated in Alzheimer’s disease.” 2015. Thesis, University of Adelaide. Accessed March 01, 2021.
http://hdl.handle.net/2440/103503.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Lim, Anne Hwee Ling. “Analysis of the subcellular localization of proteins implicated in Alzheimer’s disease.” 2015. Web. 01 Mar 2021.
Vancouver:
Lim AHL. Analysis of the subcellular localization of proteins implicated in Alzheimer’s disease. [Internet] [Thesis]. University of Adelaide; 2015. [cited 2021 Mar 01].
Available from: http://hdl.handle.net/2440/103503.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Lim AHL. Analysis of the subcellular localization of proteins implicated in Alzheimer’s disease. [Thesis]. University of Adelaide; 2015. Available from: http://hdl.handle.net/2440/103503
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Houston
8.
Zambrano Cobo, Amarayca.
Inflammation in Zebrafish Models.
Degree: MS, Biomedical Engineering, 2015, University of Houston
URL: http://hdl.handle.net/10657/4848
► The zebrafish is an outstanding model for in-vivo imaging of inflammation due to its optical translucency and the ability of transgenic lines to express fluorescent…
(more)
▼ The
zebrafish is an outstanding model for in-vivo imaging of inflammation due to its optical translucency and the ability of transgenic lines to express fluorescent proteins under specific promoters. Furthermore, the immune system of
zebrafish closely resembles that of mammals. Inflammation is the body's attempt at self-protection; the aim being to remove harmful stimuli, including damaged cells, irritants, or pathogens - and begin the healing process. This study focuses on two
zebrafish models of inflammation. The first model, Tag MPX:GFP (UWM1), which has already been established and studied, was used to investigate the possible effect of Epigallocatechin gallate (EGCG) in the inflammation process. The second model will express mCherry fluorescent protein under the Mx promoter, in order to quantify the strength or activity of upstream gene expression. The results suggest that EGCG has a favorable effect on the inflammation process. In addition, the entry clone for the Mx1-mCherry system was successfully constructed.
Advisors/Committee Members: Mohan, Chandra (advisor), Wu, Tianfu (committee member), Bondesson, Maria (committee member), Pathak, Simanta (committee member).
Subjects/Keywords: Zebrafish; Inflammation
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APA ·
Chicago ·
MLA ·
Vancouver ·
CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Zambrano Cobo, A. (2015). Inflammation in Zebrafish Models. (Masters Thesis). University of Houston. Retrieved from http://hdl.handle.net/10657/4848
Chicago Manual of Style (16th Edition):
Zambrano Cobo, Amarayca. “Inflammation in Zebrafish Models.” 2015. Masters Thesis, University of Houston. Accessed March 01, 2021.
http://hdl.handle.net/10657/4848.
MLA Handbook (7th Edition):
Zambrano Cobo, Amarayca. “Inflammation in Zebrafish Models.” 2015. Web. 01 Mar 2021.
Vancouver:
Zambrano Cobo A. Inflammation in Zebrafish Models. [Internet] [Masters thesis]. University of Houston; 2015. [cited 2021 Mar 01].
Available from: http://hdl.handle.net/10657/4848.
Council of Science Editors:
Zambrano Cobo A. Inflammation in Zebrafish Models. [Masters Thesis]. University of Houston; 2015. Available from: http://hdl.handle.net/10657/4848

University of Alberta
9.
Brewster, Daniel L.
Voltage gated potassium currents in the Mauthner and MiD2cm
cells of larval zebrafish.
Degree: MS, Department of Biological Sciences, 2012, University of Alberta
URL: https://era.library.ualberta.ca/files/wh246s285
► The escape response in zebrafish is mediated in part by the Mauthner cell and its two homologues, MiD2cm and MiD3cm. In adult fish, the Mauthner…
(more)
▼ The escape response in zebrafish is mediated in part
by the Mauthner cell and its two homologues, MiD2cm and MiD3cm. In
adult fish, the Mauthner cell fires a single action potential when
activated while the homologues fire multiple action potentials.
Their distinct firing properties are partially attributed to the
differential expression of voltage gated potassium (Kv) channels. I
am interested in determining the Kv channels associated with the
Mauthner and MiD2cm cells, and if they contain Kv1.1.
Immunohistochemistry and in situ hybridization confirmed the
expression of Kv1.1 in the Mauthner and MiD2cm cells at 48 hours
post fertilization. Electrophysiology showed these cells to contain
A-type and delayed rectifier currents but with different current
densities. Preliminary data has shown these cells have the same
firing behavior as in adults but with distinct action potential
waveforms. The presence of Kv1.1 and the distinct current
properties are suspected of regulating the neuronal
excitability.
Subjects/Keywords: Mauthner; Potassium; Zebrafish
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APA ·
Chicago ·
MLA ·
Vancouver ·
CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Brewster, D. L. (2012). Voltage gated potassium currents in the Mauthner and MiD2cm
cells of larval zebrafish. (Masters Thesis). University of Alberta. Retrieved from https://era.library.ualberta.ca/files/wh246s285
Chicago Manual of Style (16th Edition):
Brewster, Daniel L. “Voltage gated potassium currents in the Mauthner and MiD2cm
cells of larval zebrafish.” 2012. Masters Thesis, University of Alberta. Accessed March 01, 2021.
https://era.library.ualberta.ca/files/wh246s285.
MLA Handbook (7th Edition):
Brewster, Daniel L. “Voltage gated potassium currents in the Mauthner and MiD2cm
cells of larval zebrafish.” 2012. Web. 01 Mar 2021.
Vancouver:
Brewster DL. Voltage gated potassium currents in the Mauthner and MiD2cm
cells of larval zebrafish. [Internet] [Masters thesis]. University of Alberta; 2012. [cited 2021 Mar 01].
Available from: https://era.library.ualberta.ca/files/wh246s285.
Council of Science Editors:
Brewster DL. Voltage gated potassium currents in the Mauthner and MiD2cm
cells of larval zebrafish. [Masters Thesis]. University of Alberta; 2012. Available from: https://era.library.ualberta.ca/files/wh246s285

University of Bristol
10.
Campbell, Jennie S.
Investigating novel players in inflammatory cell migration to sites of tissue damage in vivo.
Degree: PhD, 2020, University of Bristol
URL: http://hdl.handle.net/1983/e5fb59ce-3715-4679-8113-97be14546369
► The use of model organisms is vital for the improvement of our understanding of dynamic immunological processes. Previous work in the developing Drosophila embryo has…
(more)
▼ The use of model organisms is vital for the improvement of our understanding of dynamic immunological processes. Previous work in the developing Drosophila embryo has demonstrated that epithelial wounding by laser ablation induces the rapid production of hydrogen peroxide (H2O2). This early damage signal activates the tyrosine kinase Src42a within the embryonic hemocytes – the innate immune cells of the organism. Src42a subsequently phosphorylates the tissue damage receptor Draper on its intracellular immunoreceptor tyrosine activation motif (ITAM) domain. This recruits a second kinase – Shark – and results in the activation of hemocytes and their subsequent migration to the damage site. As kinase activation is key to the tissue damage response of these inflammatory cells, we reasoned that other hemocyte-specific proteins may also be phosphorylated following H2O2 signalling. Therefore, a phosphoproteomics screen was conducted in order to uncover novel H2O2/Src42a regulated phosphoproteins. Using this approach, we discovered a hemocyte wound recruitment defect in embryos lacking the PTP type phosphatase Pez. This was shown to be cell-autonomous; and time-lapse imaging revealed a lack of directionality within Pez mutant hemocytes in the presence of an epithelial wound. We also uncovered dynamic localisation of Pez in vivo and it’s colocalization with Draper during the engulfment of apoptotic debris. Finally, we demonstrated that the loss of Draper and Src42a leads to the mislocalisation of Pez. To investigate whether this signalling pathway is evolutionarily conserved, tail fin clipping of 3 dpf Danio rerio larvae was utilised. Excitingly, Draper orthologue (MEGF10) morphants showed a reduction in both neutrophil and macrophage numbers recruited to the wound margin. This observation was further confirmed by the generation transient CRISPant larvae. Finally, we demonstrated that the Pez orthologue – named PTPN21 – also plays a role in inflammation in the zebrafish following gene disruption by CRISPR.
Subjects/Keywords: Drosophila; Zebrafish; Inflammation
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❌
APA ·
Chicago ·
MLA ·
Vancouver ·
CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Campbell, J. S. (2020). Investigating novel players in inflammatory cell migration to sites of tissue damage in vivo. (Doctoral Dissertation). University of Bristol. Retrieved from http://hdl.handle.net/1983/e5fb59ce-3715-4679-8113-97be14546369
Chicago Manual of Style (16th Edition):
Campbell, Jennie S. “Investigating novel players in inflammatory cell migration to sites of tissue damage in vivo.” 2020. Doctoral Dissertation, University of Bristol. Accessed March 01, 2021.
http://hdl.handle.net/1983/e5fb59ce-3715-4679-8113-97be14546369.
MLA Handbook (7th Edition):
Campbell, Jennie S. “Investigating novel players in inflammatory cell migration to sites of tissue damage in vivo.” 2020. Web. 01 Mar 2021.
Vancouver:
Campbell JS. Investigating novel players in inflammatory cell migration to sites of tissue damage in vivo. [Internet] [Doctoral dissertation]. University of Bristol; 2020. [cited 2021 Mar 01].
Available from: http://hdl.handle.net/1983/e5fb59ce-3715-4679-8113-97be14546369.
Council of Science Editors:
Campbell JS. Investigating novel players in inflammatory cell migration to sites of tissue damage in vivo. [Doctoral Dissertation]. University of Bristol; 2020. Available from: http://hdl.handle.net/1983/e5fb59ce-3715-4679-8113-97be14546369

University of Saskatchewan
11.
Kallarakavumkal Thomas, Jith.
Effects of dietary and in ovo selenomethionine exposure in zebrafish.
Degree: 2014, University of Saskatchewan
URL: http://hdl.handle.net/10388/ETD-2014-09-1744
► Selenium (Se) is an essential trace element to most living organisms, however when compared to other ingested essential trace elements Se has the lowest margin…
(more)
▼ Selenium (Se) is an essential trace element to most living organisms, however when compared to other ingested essential trace elements Se has the lowest margin of safety between essential and toxic concentrations. Oviparous vertebrates, especially fishes, are highly susceptible to dietary Se toxicity. Greater incidences of deformities and/or mortalities have been observed in F1 generation larval fishes whose parents were exposed to excess dietary Se in the form of selenomethionine (SeMet), however little information is available on effects of chronic dietary SeMet exposure to adult fish and persistent effects of in ovo SeMet exposure to F1 generation fish. This thesis investigated effects of chronic dietary exposure of excess Se in the form of SeMet on swimming performance (Ucrit), oxygen consumption (MO2), stored energy (triglycerides and glycogen), and the physiological stress response (cortisol production) in adult
zebrafish (Danio rerio), as well as immediate (incidence of deformities and mortality) and persistent (e.g. changes in Ucrit, MO2, bioenergetics, the physiological stress response and reproduction) effects of in ovo exposure to SeMet in F1 generation
zebrafish. In addition, the study investigated potential underlying mechanisms of SeMet-induced developmental toxicities in early life stages of
zebrafish using embryo microinjection.
Two separate dietary SeMet exposure studies in adult
zebrafish and two in ovo SeMet maternal transfer studies in F1 generation
zebrafish were conducted. The first dietary or in ovo exposure study explored effects of excess SeMet exposure on adult
zebrafish or the entire life cycle of F1 generation
zebrafish. The second study investigated mechanisms of observed SeMet-induced effects on adult or F1 generation
zebrafish. In the first feeding study, a significant reduction in Ucrit and greater accumulation of stored energy were observed in the excess dietary SeMet exposed groups when compared to the Se-sufficient dietary control group. The second feeding study showed a greater metabolic rate, and impaired aerobic energy metabolism and triglyceride homeostasis in adult fish fed excess dietary SeMet, which was associated with a reduction in swimming performance and accumulation of triglycerides. Embryos collected from adult
zebrafish in both dietary SeMet exposure studies were used to investigate effects of in ovo SeMet exposure on the entire life cycle of F1 generation fish. The first study showed a greater incidence of mortality, an increasing trend for deformities in F1 generation larval
zebrafish, and reduced Ucrit in F1 generation adult fish exposed to excess SeMet via in ovo maternal transfer. However, concentrations of stored energy, cortisol and reproduction were unaltered. The second study found that impaired aerobic performance might have been responsible for the reduction in Ucrit of F1 generation adult
zebrafish exposed to excess SeMet. Since there is a high variability in Se deposition among eggs via natural maternal transfer, SeMet embryo microinjection…
Advisors/Committee Members: Janz, David M., Blakley, Barry, Krone, Pat, Niyogi, Som, Drew, Murray.
Subjects/Keywords: Selenomethionine
Zebrafish
Toxicity
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❌
APA ·
Chicago ·
MLA ·
Vancouver ·
CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Kallarakavumkal Thomas, J. (2014). Effects of dietary and in ovo selenomethionine exposure in zebrafish. (Thesis). University of Saskatchewan. Retrieved from http://hdl.handle.net/10388/ETD-2014-09-1744
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Kallarakavumkal Thomas, Jith. “Effects of dietary and in ovo selenomethionine exposure in zebrafish.” 2014. Thesis, University of Saskatchewan. Accessed March 01, 2021.
http://hdl.handle.net/10388/ETD-2014-09-1744.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Kallarakavumkal Thomas, Jith. “Effects of dietary and in ovo selenomethionine exposure in zebrafish.” 2014. Web. 01 Mar 2021.
Vancouver:
Kallarakavumkal Thomas J. Effects of dietary and in ovo selenomethionine exposure in zebrafish. [Internet] [Thesis]. University of Saskatchewan; 2014. [cited 2021 Mar 01].
Available from: http://hdl.handle.net/10388/ETD-2014-09-1744.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Kallarakavumkal Thomas J. Effects of dietary and in ovo selenomethionine exposure in zebrafish. [Thesis]. University of Saskatchewan; 2014. Available from: http://hdl.handle.net/10388/ETD-2014-09-1744
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
12.
Boyle, Cody Adam.
Intergenerational Effects Of Embryonic Cocaine Exposure In Zebrafish.
Degree: MS, Biology, 2017, University of North Dakota
URL: https://commons.und.edu/theses/2174
► Cocaine addiction has both genetic and environmentally driven components. Relatively recently several groups have suggested that epigenetic regulation of gene expression might be the…
(more)
▼ Cocaine addiction has both genetic and environmentally driven components. Relatively recently several groups have suggested that epigenetic regulation of gene expression might be the mechanism linking environmental influence and inheritance in drug addiction. We have shown previously that embryonic cocaine exposure increases physiological and behavioral sensitivity to the drug in longitudinal adults. In this study, we provide evidence that the effects of embryonic pre-exposure to cocaine are intergenerational in
zebrafish. In addition, we show how gene expression in the
zebrafish telencephalon, which includes the teleost equivalent of the nucleus accumbens, changes during acute cocaine treatment during behavioral testing. These experiments outline gene expression pathways not extensively studied in the context of cocaine response. We show how embryonic exposure to cocaine affects these gene expression pathways when the longitudinal animals are acutely exposed. Finally, we show how chronic embryonic exposure affects these same pathways in 5-day old embryos.
Advisors/Committee Members: Tristan Darland.
Subjects/Keywords: Cocaine; Intergenerational; Zebrafish
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❌
APA ·
Chicago ·
MLA ·
Vancouver ·
CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Boyle, C. A. (2017). Intergenerational Effects Of Embryonic Cocaine Exposure In Zebrafish. (Masters Thesis). University of North Dakota. Retrieved from https://commons.und.edu/theses/2174
Chicago Manual of Style (16th Edition):
Boyle, Cody Adam. “Intergenerational Effects Of Embryonic Cocaine Exposure In Zebrafish.” 2017. Masters Thesis, University of North Dakota. Accessed March 01, 2021.
https://commons.und.edu/theses/2174.
MLA Handbook (7th Edition):
Boyle, Cody Adam. “Intergenerational Effects Of Embryonic Cocaine Exposure In Zebrafish.” 2017. Web. 01 Mar 2021.
Vancouver:
Boyle CA. Intergenerational Effects Of Embryonic Cocaine Exposure In Zebrafish. [Internet] [Masters thesis]. University of North Dakota; 2017. [cited 2021 Mar 01].
Available from: https://commons.und.edu/theses/2174.
Council of Science Editors:
Boyle CA. Intergenerational Effects Of Embryonic Cocaine Exposure In Zebrafish. [Masters Thesis]. University of North Dakota; 2017. Available from: https://commons.und.edu/theses/2174

University of Ottawa
13.
Boratynska, Susan.
Biological Confinement of Zebrafish Using RNAi
.
Degree: 2015, University of Ottawa
URL: http://hdl.handle.net/10393/32775
► The increasing demand for fish in the food industry has resulted in the extensive overfishing of wild fisheries. In efforts to alleviate the demand from…
(more)
▼ The increasing demand for fish in the food industry has resulted in the extensive overfishing of wild fisheries. In efforts to alleviate the demand from the food industry, genetically modified (GM) fish were developed possessing traits such as larger mass, faster growth, and increased resistance to disease. However, the greater fitness advantage of GM fish presents potential risks for wild type populations in the event of release or escape from a confined fish facility. In addition to physical barriers, it is critical to develop a genetic mechanism in order to ensure that the spread of GM transgene to the natural populations does not occur.
RNA interference (RNAi) is an endogenous mechanism used to regulate gene expression by destroying targeted mRNA molecules. Manipulation of this biological process has been successfully utilized to knockdown specific genes through the introduction of synthetic transgenes in organisms such as C. elegans, M. musculus, and D. melanogaster. Although the use of RNAi as a biological tool is still relatively new in zebrafish, recent work has explored elements of this mechanism allowing for greater knockdown efficiencies.
The deadend (dnd) gene is required for primordial germ cell (PGC) development and survival. Previous studies have shown that zebrafish dnd knockouts develop into sterile adults without disrupting somatic development. In efforts to induce sterility in zebrafish, short hairpin RNA (shRNA) constructs targeting dnd were designed to exploit the endogenous RNAi pathway. Upon qualitative analysis in transient and transgenic zebrafish subjected to the synthetic RNAi construct, a reduction in the germ cell population at early stages of development was observed. However, quantification of dnd mRNA in fish from the same time points did not show significant changes in expression levels compared to their wildtype counterparts. Adult fish subjected to the transgene construct produced viable gametes.
The use of RNAi as a tool for bioconfinement relies on sterility among all individuals subjected to the shRNA bearing transgene. Based on the results obtained, the verdict is still unclear as to whether shRNA is a viable mechanism for large scale bioconfinement.
Subjects/Keywords: RNAi;
dnd;
Zebrafish
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❌
APA ·
Chicago ·
MLA ·
Vancouver ·
CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Boratynska, S. (2015). Biological Confinement of Zebrafish Using RNAi
. (Thesis). University of Ottawa. Retrieved from http://hdl.handle.net/10393/32775
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Boratynska, Susan. “Biological Confinement of Zebrafish Using RNAi
.” 2015. Thesis, University of Ottawa. Accessed March 01, 2021.
http://hdl.handle.net/10393/32775.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Boratynska, Susan. “Biological Confinement of Zebrafish Using RNAi
.” 2015. Web. 01 Mar 2021.
Vancouver:
Boratynska S. Biological Confinement of Zebrafish Using RNAi
. [Internet] [Thesis]. University of Ottawa; 2015. [cited 2021 Mar 01].
Available from: http://hdl.handle.net/10393/32775.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Boratynska S. Biological Confinement of Zebrafish Using RNAi
. [Thesis]. University of Ottawa; 2015. Available from: http://hdl.handle.net/10393/32775
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Deakin University
14.
Mohd Noor, Suzita.
Role of Jak2/Stat5/Cis pathway components in zebrafish development.
Degree: School of Medicine, 2011, Deakin University
URL: http://hdl.handle.net/10536/DRO/DU:30036712
► By utilizing the zebrafish as a research model, the function of specific cell signalling pathway components in development was investigated. This revealed new roles for…
(more)
▼ By utilizing the
zebrafish as a research model, the function of specific cell signalling pathway components in development was investigated. This revealed new roles for the so-called Jak2/Stat5/Cis pathway in blood and sensory organ development, and a conserved prolactin pathway despite divergent functions between species.
Advisors/Committee Members: Ward, Alister.
Subjects/Keywords: embryology; cytokines; zebrafish
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APA ·
Chicago ·
MLA ·
Vancouver ·
CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Mohd Noor, S. (2011). Role of Jak2/Stat5/Cis pathway components in zebrafish development. (Thesis). Deakin University. Retrieved from http://hdl.handle.net/10536/DRO/DU:30036712
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Mohd Noor, Suzita. “Role of Jak2/Stat5/Cis pathway components in zebrafish development.” 2011. Thesis, Deakin University. Accessed March 01, 2021.
http://hdl.handle.net/10536/DRO/DU:30036712.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Mohd Noor, Suzita. “Role of Jak2/Stat5/Cis pathway components in zebrafish development.” 2011. Web. 01 Mar 2021.
Vancouver:
Mohd Noor S. Role of Jak2/Stat5/Cis pathway components in zebrafish development. [Internet] [Thesis]. Deakin University; 2011. [cited 2021 Mar 01].
Available from: http://hdl.handle.net/10536/DRO/DU:30036712.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Mohd Noor S. Role of Jak2/Stat5/Cis pathway components in zebrafish development. [Thesis]. Deakin University; 2011. Available from: http://hdl.handle.net/10536/DRO/DU:30036712
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Manchester
15.
Oltrabella, Francesca.
Investigation of OCRL1 and its interaction partners in zebrafish.
Degree: PhD, 2014, University of Manchester
URL: https://www.research.manchester.ac.uk/portal/en/theses/investigation-of-ocrl1-and-its-interaction-partners-in-zebrafish(77a97cd8-f030-4c4e-93cc-fe4e2ea44ee6).html
;
https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.764293
► Oculocerebrorenal syndrome of Lowe is a rare X-linked disorder caused by mutation of the inositol 5-phosphatase OCRL1. Lowe Syndrome manifests as renal tubular dysfunction, neurological…
(more)
▼ Oculocerebrorenal syndrome of Lowe is a rare X-linked disorder caused by mutation of the inositol 5-phosphatase OCRL1. Lowe Syndrome manifests as renal tubular dysfunction, neurological and ocular defects. OCRL1 uses its catalytic domain to hydrolyze two phosphoinositide species, PI(4,5)P2 and PI3,4,5)P3. It is involved in regulation of membrane trafficking, actin dynamics, cytokinesis and ciliogenesis. OCRL1 interacts with IPIP27A and B, which have been shown to be key players in endocytic trafficking in mammalian cells, specifically in the recycling of proteins from early and recycling endosomes to both the plasma membrane and trans-Golgi network. It has been proposed that defective endocytic trafficking may be responsible for the renal tubulopathy seen in Lowe Syndrome patients, characterized by low molecular weight proteinuria and aminoaciduria, but this hypothesis has yet to be tested. Using zebrafish as a model for Lowe syndrome, we show that depletion of OCRL1 can indeed cause defects in endocytosis in the renal tubule. This coincides with a reduction in levels of the multi-ligand receptor megalin, reduced abundance of the endocytic apparatus and increased numbers of enlarged lysosomes in the kidney tubular cells. We also show that knocking-down Pip5K in the OCRL1 mutants to rebalance PI(4,5)P2 levels can rescue the endocytic defect. This indicates that tight control of PI(4,5)P2 level is essential for efficient endocytic trafficking in vivo. Importantly, this finding suggests that Pip5K may be a valuable therapeutic target for patients with Lowe Syndrome. To further characterize the molecular mechanisms by which OCRL1 promotes endocytosis, we have focused on the recently identified OCRL1 interaction partners IPIP27A and B, which are known to function in endocytosis and receptor recycling. Here we report identification and characterization of the zebrafish Ipip27s, including analysis of conservation and expression profiles. To assess Ipip27s function in vivo, KO zebrafish lines were generated using TALENs. This was successful for Ipip27A, but so far not for Ipip27B. Functional analysis using the Ipip27A KO line and KD with morpholinos revealed that both Ipip27s contribute to neural development and may participate in ciliogenesis. Moreover, preliminary analysis indicates an important role for Ipip27A within the endocytic pathway in the kidney tubule, where its loss phenocopies many aspects of the OCRL1 mutant phenotype.
Subjects/Keywords: 570; ZEBRAFISH; OCRL1
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APA (6th Edition):
Oltrabella, F. (2014). Investigation of OCRL1 and its interaction partners in zebrafish. (Doctoral Dissertation). University of Manchester. Retrieved from https://www.research.manchester.ac.uk/portal/en/theses/investigation-of-ocrl1-and-its-interaction-partners-in-zebrafish(77a97cd8-f030-4c4e-93cc-fe4e2ea44ee6).html ; https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.764293
Chicago Manual of Style (16th Edition):
Oltrabella, Francesca. “Investigation of OCRL1 and its interaction partners in zebrafish.” 2014. Doctoral Dissertation, University of Manchester. Accessed March 01, 2021.
https://www.research.manchester.ac.uk/portal/en/theses/investigation-of-ocrl1-and-its-interaction-partners-in-zebrafish(77a97cd8-f030-4c4e-93cc-fe4e2ea44ee6).html ; https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.764293.
MLA Handbook (7th Edition):
Oltrabella, Francesca. “Investigation of OCRL1 and its interaction partners in zebrafish.” 2014. Web. 01 Mar 2021.
Vancouver:
Oltrabella F. Investigation of OCRL1 and its interaction partners in zebrafish. [Internet] [Doctoral dissertation]. University of Manchester; 2014. [cited 2021 Mar 01].
Available from: https://www.research.manchester.ac.uk/portal/en/theses/investigation-of-ocrl1-and-its-interaction-partners-in-zebrafish(77a97cd8-f030-4c4e-93cc-fe4e2ea44ee6).html ; https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.764293.
Council of Science Editors:
Oltrabella F. Investigation of OCRL1 and its interaction partners in zebrafish. [Doctoral Dissertation]. University of Manchester; 2014. Available from: https://www.research.manchester.ac.uk/portal/en/theses/investigation-of-ocrl1-and-its-interaction-partners-in-zebrafish(77a97cd8-f030-4c4e-93cc-fe4e2ea44ee6).html ; https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.764293

Rice University
16.
Rivera Longsworth, Gia Francesca.
Expanding the Enzymatic Activity of the Programmable Endonuclease Cas9.
Degree: MA, Natural Sciences, 2018, Rice University
URL: http://hdl.handle.net/1911/105841
► The CRISPR-Cas9 gene editing system has revolutionized our ability to make targeted mutations in a variety of organisms. In zebrafish, we are able to use…
(more)
▼ The CRISPR-Cas9 gene editing system has revolutionized our ability to make targeted mutations in a variety of organisms. In
zebrafish, we are able to use microinjections of Cas9 protein or mRNA alongside guide RNAs (gRNA) to create targeted insertions and deletions (indels) at a frequency sufficient for recovery of loss of function alleles. Combined with the many practical advantages of working with
zebrafish, CRISPR-Cas9 has enabled many labs to study gene function at a lowered cost. However, there are improvements that can be made to facilitate the knock in of sequences into the genome, a process which remains inefficient in
zebrafish due to a low frequency of homology directed repair (HDR), a mechanism necessary for knock in. Our aim is to increase the rate of HDR in
zebrafish by modifying Cas9 using two approaches. First, we hypothesize that the long latency period of the Cas9-DNA complex taken with the short cell cycle of the early
zebrafish embryo is biasing the repair mechanism against HDR, so I have engineered a CL1 degron-tagged Cas9 (DegCas9) to destabilize the Cas9 protein. A second approach to increasing the rate of knock in is to reduce the number of molecules involved in the HDR process by using an RNA template for recombination. We hypothesize that the rate of HDR can be improved if the recombination template remains in proximity to the DSB. To this aim, I have created a reverse-transcriptase Cas9 fusion and modified gRNAs that include the targeting sequence for recombination. Our goal is to create a Cas9 system that efficiently uses an RNA template for recombination. Results indicate that DegCas9 is capable of inducing mutations at a reduced rate, and that the modified gRNAs for the RTCas9 system do not reduce the effectiveness of Cas9 function. Further studies will include analyzing the spectrum of lesions induced by DegCas9 and creating alternative versions of DegCas9 and RTCas9 using different domains. If successful, these Cas9 constructs will expand the toolkit available for genetic engineering in
zebrafish.
Advisors/Committee Members: Wagner, Daniel (advisor).
Subjects/Keywords: zebrafish; Cas9; CRISPR
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APA ·
Chicago ·
MLA ·
Vancouver ·
CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Rivera Longsworth, G. F. (2018). Expanding the Enzymatic Activity of the Programmable Endonuclease Cas9. (Masters Thesis). Rice University. Retrieved from http://hdl.handle.net/1911/105841
Chicago Manual of Style (16th Edition):
Rivera Longsworth, Gia Francesca. “Expanding the Enzymatic Activity of the Programmable Endonuclease Cas9.” 2018. Masters Thesis, Rice University. Accessed March 01, 2021.
http://hdl.handle.net/1911/105841.
MLA Handbook (7th Edition):
Rivera Longsworth, Gia Francesca. “Expanding the Enzymatic Activity of the Programmable Endonuclease Cas9.” 2018. Web. 01 Mar 2021.
Vancouver:
Rivera Longsworth GF. Expanding the Enzymatic Activity of the Programmable Endonuclease Cas9. [Internet] [Masters thesis]. Rice University; 2018. [cited 2021 Mar 01].
Available from: http://hdl.handle.net/1911/105841.
Council of Science Editors:
Rivera Longsworth GF. Expanding the Enzymatic Activity of the Programmable Endonuclease Cas9. [Masters Thesis]. Rice University; 2018. Available from: http://hdl.handle.net/1911/105841

University of Arizona
17.
Todd, Douglas Wallace.
Zebrafish Video Analysis System for High-Throughput Drug Assay
.
Degree: 2016, University of Arizona
URL: http://hdl.handle.net/10150/623150
► Zebrafish swimming behavior is used in a new, automated drug assay system as a biomarker to measure drug efficiency to prevent or restore hearing loss.…
(more)
▼ Zebrafish swimming behavior is used in a new, automated drug assay system as a biomarker to measure drug efficiency to prevent or restore hearing loss. This system records video of
zebrafish larvae under infrared lighting using Raspberry Pi cameras and measures fish swimming behavior. This automated system significantly reduces the operator time required to process experiments in parallel. Multiple tanks, each consisting of sixteen experiments are operated in parallel. Once a set of experiments starts, all data transfer and processing operations are automatic. A web interface allows the operator to configure, monitor and control the experiments and review reports. Ethernet connects the various hardware components, allowing loose coupling of the distributed software used to schedule and run the experiments. The operator can configure the data processing to be done on the local computer or offloaded to a high-performance computer cluster to achieve even higher throughput. Computationally efficient image processing algorithms provided automated
zebrafish detection and motion analysis. Quantitative assessment of error in the position and orientation of the detected fish uses manual data analysis by human observers as the reference. The system error in orientation and position is comparable to human inter-operator error.
Advisors/Committee Members: Rodríguez, Jeffrey J (advisor), Rodríguez, Jeffrey J. (committeemember), Powers, Linda S. (committeemember), Tharp, Hal S. (committeemember).
Subjects/Keywords: zebrafish;
image analysis
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APA ·
Chicago ·
MLA ·
Vancouver ·
CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Todd, D. W. (2016). Zebrafish Video Analysis System for High-Throughput Drug Assay
. (Masters Thesis). University of Arizona. Retrieved from http://hdl.handle.net/10150/623150
Chicago Manual of Style (16th Edition):
Todd, Douglas Wallace. “Zebrafish Video Analysis System for High-Throughput Drug Assay
.” 2016. Masters Thesis, University of Arizona. Accessed March 01, 2021.
http://hdl.handle.net/10150/623150.
MLA Handbook (7th Edition):
Todd, Douglas Wallace. “Zebrafish Video Analysis System for High-Throughput Drug Assay
.” 2016. Web. 01 Mar 2021.
Vancouver:
Todd DW. Zebrafish Video Analysis System for High-Throughput Drug Assay
. [Internet] [Masters thesis]. University of Arizona; 2016. [cited 2021 Mar 01].
Available from: http://hdl.handle.net/10150/623150.
Council of Science Editors:
Todd DW. Zebrafish Video Analysis System for High-Throughput Drug Assay
. [Masters Thesis]. University of Arizona; 2016. Available from: http://hdl.handle.net/10150/623150

University of North Texas
18.
Adhikari, Prem R.
Proteomic Responses in the Gill of Zebrafish Following Exposure to Ibuprofen and Naproxen.
Degree: 2012, University of North Texas
URL: https://digital.library.unt.edu/ark:/67531/metadc149557/
► Non-steroidal anti-inflammatory drugs (NSAIDs) are among the most abundant environmental pharmaceutical contaminants. In this study, a proteomic analysis was conducted to identify proteins differentially expressed…
(more)
▼ Non-steroidal anti-inflammatory drugs (NSAIDs) are among the most abundant environmental pharmaceutical contaminants. In this study, a proteomic analysis was conducted to identify proteins differentially expressed in gill tissue of
zebrafish (Danio rerio) after a 14-day exposure to the NSAIDs ibuprofen or naproxen. A total of 104 proteins with altered expression as indicated by 2-dimensional electrophoresis were analyzed by liquid chromatography with ion trap mass spectrometry (MS/MS). A total of 14 proteins fulfilled our requirements for identification which included consistency among replicate gels as well as successful MS/MS ion searches with the MASCOT database. The most prominent feature of the differential protein expression observed after NSAID exposure was an up-regulation of proteins belonging to the globin family which are involved in the transport of oxygen from gills and availability of heme molecules required for synthesis of cyclooxygenase. Differential expression was observed at exposure concentrations as low as 1-10 µg/L indicating that altered gene expression may occur in fish subjected to environmentally realistic levels of NSAID exposure.
Advisors/Committee Members: Venables, Barney J., Chapman, Kent Dean, Huggett, Duane B., Root, Douglas D., Point, Tom La.
Subjects/Keywords: Proteomics; NSAID; zebrafish
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University of North Texas
19.
Alsrhani, Abdullah Falleh.
Novel Role of Trypsin in Zebrafish.
Degree: 2013, University of North Texas
URL: https://digital.library.unt.edu/ark:/67531/metadc271771/
► It has been shown previously in our laboratory that zebrafish produce trypsin from their gills when they are under stress, and this trypsin is involved…
(more)
▼ It has been shown previously in our laboratory that
zebrafish produce trypsin from their gills when they are under stress, and this trypsin is involved in thrombocyte activation via PAR2 during gill bleeding. In this study, I investigated another role of the trypsin that is secreted from
zebrafish. This investigation has demonstrated a novel role of trypsin in
zebrafish. Not only did this investigation demonstrate the role of trypsin in
zebrafish behavior, but also it showed that PAR2 might be the receptor that is involved in trypsin-mediated behavioral response. In addition, we have shown that Gq and ERK inhibitors are able to block the trypsin pathway and prevent the escaping behavior. Finally, the results of this investigation suggest that the cells that respond to trypsin are surface cells, which have an appearance similar to that of neuromast cells.
Advisors/Committee Members: Jagadeeswaran, Pudur, O'Donovan, Gerard A., Benjamin, Robert C..
Subjects/Keywords: Trypsin; zebrafish; thrombocyte
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University of Oregon
20.
Schlomann, Brandon.
Learning Biophysical Rules of Gut Bacterial Communities Through Live Imaging of Zebrafish.
Degree: PhD, Department of Physics, 2020, University of Oregon
URL: https://scholarsbank.uoregon.edu/xmlui/handle/1794/25611
► Vast communities of microorganisms inhabit the gastrointestinal tracts of humans and other animals, where they influence diverse aspects of animal health and disease. Our understanding…
(more)
▼ Vast communities of microorganisms inhabit the gastrointestinal tracts of humans and other animals, where they influence diverse aspects of animal health and disease. Our understanding of the types of microbes present in the intestine and the genes that they carry has grown tremendously in recent years, but despite this progress, we are still unable to predict the abundances of microbial strains in the gut and their impact on host phenotypes. This deficiency limits our abilities to uncover causal mechanisms mediating host-microbe interactions and to rationally design novel therapeutic strategies. A major barrier to achieving these goals is our limited ability to experimentally probe the spatial organization of gut bacterial communities, which is thought to be a key driver of microbiota dynamics, but which is largely inaccessible in most systems. This dissertation work addresses these knowledge gaps by combining quantitative theory with controlled experiments in a model system that can uniquely surmount these technical challenges. The larval
zebrafish is an optically transparent, model vertebrate that is amenable to live imaging studies, in which bacteria in the gut can be directly visualized and studied in situ. Through this approach, we discovered that the biophysical properties of bacteria in the gut, especially their aggregation and swimming behaviors, coupled to intestinal fluid flows, determine in robust but probabilistic ways several large-scale features of whole bacterial populations. These features include global spatial distributions of bacteria throughout the gut, bacterial population dynamics, both at baseline and in response to perturbations like antibiotics, and the ability of bacteria to stimulate immune responses. Through the study and validation of phenomenological models, we argue that these effects are generic and manifest in other animals, including humans, and suggest new strategies to harness these effects for precision microbiome engineering.
Advisors/Committee Members: Parthasarathy, Raghuveer (advisor).
Subjects/Keywords: bacteria; microbiota; zebrafish
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❌
APA ·
Chicago ·
MLA ·
Vancouver ·
CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Schlomann, B. (2020). Learning Biophysical Rules of Gut Bacterial Communities Through Live Imaging of Zebrafish. (Doctoral Dissertation). University of Oregon. Retrieved from https://scholarsbank.uoregon.edu/xmlui/handle/1794/25611
Chicago Manual of Style (16th Edition):
Schlomann, Brandon. “Learning Biophysical Rules of Gut Bacterial Communities Through Live Imaging of Zebrafish.” 2020. Doctoral Dissertation, University of Oregon. Accessed March 01, 2021.
https://scholarsbank.uoregon.edu/xmlui/handle/1794/25611.
MLA Handbook (7th Edition):
Schlomann, Brandon. “Learning Biophysical Rules of Gut Bacterial Communities Through Live Imaging of Zebrafish.” 2020. Web. 01 Mar 2021.
Vancouver:
Schlomann B. Learning Biophysical Rules of Gut Bacterial Communities Through Live Imaging of Zebrafish. [Internet] [Doctoral dissertation]. University of Oregon; 2020. [cited 2021 Mar 01].
Available from: https://scholarsbank.uoregon.edu/xmlui/handle/1794/25611.
Council of Science Editors:
Schlomann B. Learning Biophysical Rules of Gut Bacterial Communities Through Live Imaging of Zebrafish. [Doctoral Dissertation]. University of Oregon; 2020. Available from: https://scholarsbank.uoregon.edu/xmlui/handle/1794/25611

University of Bristol
21.
Campbell, Jennie S.
Investigating novel players in inflammatory cell migration to sites of tissue damage in vivo.
Degree: PhD, 2020, University of Bristol
URL: https://research-information.bris.ac.uk/en/studentTheses/e5fb59ce-3715-4679-8113-97be14546369
;
https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.801589
► The use of model organisms is vital for the improvement of our understanding of dynamic immunological processes. Previous work in the developing Drosophila embryo has…
(more)
▼ The use of model organisms is vital for the improvement of our understanding of dynamic immunological processes. Previous work in the developing Drosophila embryo has demonstrated that epithelial wounding by laser ablation induces the rapid production of hydrogen peroxide (H2O2). This early damage signal activates the tyrosine kinase Src42a within the embryonic hemocytes – the innate immune cells of the organism. Src42a subsequently phosphorylates the tissue damage receptor Draper on its intracellular immunoreceptor tyrosine activation motif (ITAM) domain. This recruits a second kinase – Shark – and results in the activation of hemocytes and their subsequent migration to the damage site. As kinase activation is key to the tissue damage response of these inflammatory cells, we reasoned that other hemocyte-specific proteins may also be phosphorylated following H2O2 signalling. Therefore, a phosphoproteomics screen was conducted in order to uncover novel H2O2/Src42a regulated phosphoproteins. Using this approach, we discovered a hemocyte wound recruitment defect in embryos lacking the PTP type phosphatase Pez. This was shown to be cell-autonomous; and time-lapse imaging revealed a lack of directionality within Pez mutant hemocytes in the presence of an epithelial wound. We also uncovered dynamic localisation of Pez in vivo and it’s colocalization with Draper during the engulfment of apoptotic debris. Finally, we demonstrated that the loss of Draper and Src42a leads to the mislocalisation of Pez. To investigate whether this signalling pathway is evolutionarily conserved, tail fin clipping of 3 dpf Danio rerio larvae was utilised. Excitingly, Draper orthologue (MEGF10) morphants showed a reduction in both neutrophil and macrophage numbers recruited to the wound margin. This observation was further confirmed by the generation transient CRISPant larvae. Finally, we demonstrated that the Pez orthologue – named PTPN21 – also plays a role in inflammation in the zebrafish following gene disruption by CRISPR.
Subjects/Keywords: Drosophila; Zebrafish; Inflammation
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❌
APA ·
Chicago ·
MLA ·
Vancouver ·
CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Campbell, J. S. (2020). Investigating novel players in inflammatory cell migration to sites of tissue damage in vivo. (Doctoral Dissertation). University of Bristol. Retrieved from https://research-information.bris.ac.uk/en/studentTheses/e5fb59ce-3715-4679-8113-97be14546369 ; https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.801589
Chicago Manual of Style (16th Edition):
Campbell, Jennie S. “Investigating novel players in inflammatory cell migration to sites of tissue damage in vivo.” 2020. Doctoral Dissertation, University of Bristol. Accessed March 01, 2021.
https://research-information.bris.ac.uk/en/studentTheses/e5fb59ce-3715-4679-8113-97be14546369 ; https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.801589.
MLA Handbook (7th Edition):
Campbell, Jennie S. “Investigating novel players in inflammatory cell migration to sites of tissue damage in vivo.” 2020. Web. 01 Mar 2021.
Vancouver:
Campbell JS. Investigating novel players in inflammatory cell migration to sites of tissue damage in vivo. [Internet] [Doctoral dissertation]. University of Bristol; 2020. [cited 2021 Mar 01].
Available from: https://research-information.bris.ac.uk/en/studentTheses/e5fb59ce-3715-4679-8113-97be14546369 ; https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.801589.
Council of Science Editors:
Campbell JS. Investigating novel players in inflammatory cell migration to sites of tissue damage in vivo. [Doctoral Dissertation]. University of Bristol; 2020. Available from: https://research-information.bris.ac.uk/en/studentTheses/e5fb59ce-3715-4679-8113-97be14546369 ; https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.801589

NSYSU
22.
Mou, Yu-Zheng.
CoupTF1b functions in vascular development in zebrafish.
Degree: Master, Biological Sciences, 2014, NSYSU
URL: http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0702114-013814
► Genetic programs and signaling pathways are required for proper growth and patterning of blood vessels, however, little known about the transcription factors functioning in vein…
(more)
▼ Genetic programs and signaling pathways are required for proper growth and patterning of blood vessels, however, little known about the transcription factors functioning in vein identity and intersegmental vessels (ISV) patterning in
zebrafish.
The orphan nuclear receptor Chicken ovalbumin upstream promoter transcription factor II (CoupTFII) positively regulates vein identity in mice. Here we show that the CoupTF1b is important for vein and tip cell identity. Comparison of amino acids sequence and phylogenetic analysis of CoupTF1b orthologs in different species suggesting the CoupTF1b is conserved among vertebrates. CoupTF1b mRNA has a spatiotemporal expression pattern in ventral lateral mesoderm consistent with a role in early vascular specification. Morpholino
knockdown of coupTFIb results in a decrease in both venous fluorescent signals and expression of the vein specific marker flt4 and mrc1. These data suggest that CoupTF1b has novel role in promoting vein identity. In addition, we show loss of CoupTF1b impairs ISV growth, suggesting that CoupTF1b also has an important role in controlling ISV growth. We have confirmed those vascular defects are CoupTF1b specific by using two different types of morpholinos knockdown strategies (ATG and splicing morpholinos). To address whether the decrease in vein signals and the growth defect in ISV result from cell death, we performed TUNEL assay. We showed that an increase in non-specific cell death after morpholino injection is possible cause of the observed vascular phenotype. We further test CoupTF1b likely interact with the Notch signaling pathway to control vascular development. Together, we show CoupTF1b plays an indispensable role for vascular development in
zebrafish.
Advisors/Committee Members: Yung-Jen, Chuang (chair), Chang-Yi, Wu (committee member), Ming-Hong Tai (chair).
Subjects/Keywords: intersegmental vessels; CoupTF1b; angiogenesis; zebrafish
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❌
APA ·
Chicago ·
MLA ·
Vancouver ·
CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Mou, Y. (2014). CoupTF1b functions in vascular development in zebrafish. (Thesis). NSYSU. Retrieved from http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0702114-013814
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Mou, Yu-Zheng. “CoupTF1b functions in vascular development in zebrafish.” 2014. Thesis, NSYSU. Accessed March 01, 2021.
http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0702114-013814.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Mou, Yu-Zheng. “CoupTF1b functions in vascular development in zebrafish.” 2014. Web. 01 Mar 2021.
Vancouver:
Mou Y. CoupTF1b functions in vascular development in zebrafish. [Internet] [Thesis]. NSYSU; 2014. [cited 2021 Mar 01].
Available from: http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0702114-013814.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Mou Y. CoupTF1b functions in vascular development in zebrafish. [Thesis]. NSYSU; 2014. Available from: http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0702114-013814
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Oregon State University
23.
Chlebowski, Anna C.
Utilization of the Zebrafish Model for Investigating Nitrated Polycyclic Aromatic Hydrocarbon Developmental Toxicity.
Degree: PhD, Toxicology, 2017, Oregon State University
URL: http://hdl.handle.net/1957/60687
► Polycyclic aromatic hydrocarbons (PAHs) are among the most widely known and studied environmental contaminants, originating from a range of natural and anthropogenic sources. PAHs are…
(more)
▼ Polycyclic aromatic hydrocarbons (PAHs) are among the most widely known and studied environmental contaminants, originating from a range of natural and anthropogenic sources. PAHs are known to occur in the environment as complex mixtures, containing both unsubstituted PAHs, as well as a range of PAH derivatives. Among the less-studied of these derivative PAH classes are nitrated PAHs (NPAHs). NPAHs are known to form from atmospheric reactions with PAHs and can be found in the environment in a variety of matrices. Many NPAHs are known to be mutagenic, in some cases more so than the corresponding unsubstituted PAH. Less is known about the toxicity of NPAHs in whole-animal systems and for non-cancer endpoints, in particular with regard to the developmental toxicity and metabolism across a wide number of NPAH compounds, in a consistent model system.
One of the major challenges in studying PAHs, and related compounds, is the high hydrophobicity and low water solubility of these compounds, which can result in losses due to partitioning of the analytes out of the aqueous phase and on to the walls of the container or exposure vessel. Numerous in vitro and in vivo models utilize plastic plates as exposure vessels, including the use of polystyrene 96-well plates for developmental toxicity testing in the developing
zebrafish (Danio rerio) model. We directly measured the losses which occur due to sorption to the polystyrene plates during
zebrafish testing for a set of PAHs and NPAHs. Sorptive losses in some instances were greater than fifty percent, in particular for the lower of the two exposure concentrations tested. These sorptive losses decrease the concentration of chemical available to the
zebrafish embryos, and therefore impact the interpretation of dose-response toxicity data. In an attempt to create a predictive model for sorptive losses, the measured sorption was modeled against the log K[subscript ow], molecular weight, and subcooled liquid solubilities of the corresponding compounds. The correlations between subcooled liquid solubility and PAH sorption was statistically significant (p<0.05) for both concentrations tested, as well as molecular weight at the higher concentrations tested. However, none of the correlations were statistically significant for NPAH sorption, indicating a need for increased research in this area.
We utilized the developing
zebrafish model to investigate the developmental toxicity, and potential contributing mechanisms of action, of a suite of 27 NPAHs, as well as 10 heterocyclic PAHs (HPAHs) and 2 amino-PAHs (potential metabolites of NPAHs). Results from the toxicity screen indicate that NPAHs and HPAHs have a wide range of bioactivities in the developing
zebrafish, from non-toxic at the concentrations tested to acutely toxic at sub-micromolar exposure concentrations. Activation of the aryl hydrocarbon receptor (AHR) pathway was investigated using a transgenic reporter
zebrafish line and morpholino oligonucleotide knockdown to isolate specific isoforms of the AHR. The compounds…
Advisors/Committee Members: Simonich, Staci L. M. (advisor), Field, Jennifer (committee member).
Subjects/Keywords: zebrafish; Polycyclic aromatic hydrocarbons – Toxicology
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❌
APA ·
Chicago ·
MLA ·
Vancouver ·
CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Chlebowski, A. C. (2017). Utilization of the Zebrafish Model for Investigating Nitrated Polycyclic Aromatic Hydrocarbon Developmental Toxicity. (Doctoral Dissertation). Oregon State University. Retrieved from http://hdl.handle.net/1957/60687
Chicago Manual of Style (16th Edition):
Chlebowski, Anna C. “Utilization of the Zebrafish Model for Investigating Nitrated Polycyclic Aromatic Hydrocarbon Developmental Toxicity.” 2017. Doctoral Dissertation, Oregon State University. Accessed March 01, 2021.
http://hdl.handle.net/1957/60687.
MLA Handbook (7th Edition):
Chlebowski, Anna C. “Utilization of the Zebrafish Model for Investigating Nitrated Polycyclic Aromatic Hydrocarbon Developmental Toxicity.” 2017. Web. 01 Mar 2021.
Vancouver:
Chlebowski AC. Utilization of the Zebrafish Model for Investigating Nitrated Polycyclic Aromatic Hydrocarbon Developmental Toxicity. [Internet] [Doctoral dissertation]. Oregon State University; 2017. [cited 2021 Mar 01].
Available from: http://hdl.handle.net/1957/60687.
Council of Science Editors:
Chlebowski AC. Utilization of the Zebrafish Model for Investigating Nitrated Polycyclic Aromatic Hydrocarbon Developmental Toxicity. [Doctoral Dissertation]. Oregon State University; 2017. Available from: http://hdl.handle.net/1957/60687

Universiteit Utrecht
24.
Stumpf, Miriam.
Interplay of PTEN subcellular localization and catalytic activities in vivo.
Degree: 2016, Universiteit Utrecht
URL: http://dspace.library.uu.nl:8080/handle/1874/334160
► This thesis describes the use of mammalian cells, S. cerevisiae and D. rerio to unravel the complex interplay of PTEN subcellular localization and catalytic activities.…
(more)
▼ This thesis describes the use of mammalian cells, S. cerevisiae and D. rerio to unravel the complex interplay of PTEN subcellular localization and catalytic activities. In Chapter 1 we provide a general introduction to the PI3K/Akt(PKB)/PTEN axis, PTEN phosphatase-dependent and –independent functions and regulation of those functions through changes in protein conformation and subcellular localization. Last, we give a short résumé on the role of PTEN in human health and disease. In Chapter 2, we give an overview of developmental and pathological processes that have been successfully studied in
zebrafish pten models. Further, we introduce techniques that we and others have developed for modulation of Pten expression in vivo and for the establishment of
zebrafish cell lines from tumors. In Chapter 3 we performed a comprehensive mutational and functional analysis of the PTEN N-terminus. To analyze the contribution of this region to PTEN tumor suppressor function in vivo, we studied a panel of tumor-related mutations, employing S.cerevisiae and mammalian cells. We found that most tumor-related N-terminal PTEN mutations that lead to a loss of PIP3-phosphatase activity also showed impaired nuclear localization. This suggests that PIP3 catalytic activity and nuclear localization of PTEN are coordinated by the PTEN N-terminus in an overlapping manner. Our results from soft agar colony formation assays further indicated that both the PTEN PIP3 phosphatase activity and the PTEN capacity to accumulate in the nucleus were important for the full tumor suppression capacity of PTEN. In Chapter 4 we focused on the classical protein import pathway as a possible major nuclear transport mechanism for PTEN and, by immunofluorescence and confocal microscopy performed in mammalian cells, we identified importin alpha3 as a factor involved in PTEN nuclear translocation. Further, we found that the PTEN N-terminal region (residues 1-32) could mediate nuclear accumulation of PTEN through interactions with nuclear transporters. Systematic introduction of point mutations in the importin alpha 3 substrate binding pockets allowed us to further narrow down the region of importin alpha 3 that is important for nuclear accumulation of PTEN to the minor binding pocket. Using the
zebrafish as a model organism, in Chapter 5 we unveil a differential requirement of Pten lipid and protein phosphatase activity during embryonic development. To this end, we performed rescue assays. We propose that the role of Pten during angiogenesis mainly consists of suppressing PI3K signaling via its lipid phosphatase activity, whereas the complex process of embryonic development requires lipid and protein phosphatase activity of Pten. In Chapter 6 we characterize the subcellular localization and the functional consquences of the expression of open conformation PTEN. The functional hyperactivity of open conformation PTEN in comparison to PTEN wild type in our model seemed to result predominantly from its enhanced recruitment to the cytoplasmic membrane. In conclusion, we…
Advisors/Committee Members: Bakkers, Jeroen, den Hertog, J..
Subjects/Keywords: PTEN; Zebrafish; Cancer; Angiogenesis; Importins
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APA ·
Chicago ·
MLA ·
Vancouver ·
CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Stumpf, M. (2016). Interplay of PTEN subcellular localization and catalytic activities in vivo. (Doctoral Dissertation). Universiteit Utrecht. Retrieved from http://dspace.library.uu.nl:8080/handle/1874/334160
Chicago Manual of Style (16th Edition):
Stumpf, Miriam. “Interplay of PTEN subcellular localization and catalytic activities in vivo.” 2016. Doctoral Dissertation, Universiteit Utrecht. Accessed March 01, 2021.
http://dspace.library.uu.nl:8080/handle/1874/334160.
MLA Handbook (7th Edition):
Stumpf, Miriam. “Interplay of PTEN subcellular localization and catalytic activities in vivo.” 2016. Web. 01 Mar 2021.
Vancouver:
Stumpf M. Interplay of PTEN subcellular localization and catalytic activities in vivo. [Internet] [Doctoral dissertation]. Universiteit Utrecht; 2016. [cited 2021 Mar 01].
Available from: http://dspace.library.uu.nl:8080/handle/1874/334160.
Council of Science Editors:
Stumpf M. Interplay of PTEN subcellular localization and catalytic activities in vivo. [Doctoral Dissertation]. Universiteit Utrecht; 2016. Available from: http://dspace.library.uu.nl:8080/handle/1874/334160

NSYSU
25.
Song , Yi-Chun.
Coral-derived natural marine compound GB9 inhibits vascular development in zebrafish.
Degree: Master, Biological Sciences, 2017, NSYSU
URL: http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0716117-115842
► Vascular development is important for vertebrates, and genetic control vascular patterning has been intensively characterized. In addition, many studies have been show environmental hormones and…
(more)
▼ Vascular development is important for vertebrates, and genetic control vascular patterning has been intensively characterized. In addition, many studies have been show environmental hormones and chemical compounds affect vascular growth. Natural marine compound GB9 was isolated from the marine soft coral Capnella imbricate, the study show GB9 had anti-neuroinflammatory and anti-nociceptive effects. However, the effect of GB9 on blood vessels is still unknown. In this study, we use transgene
zebrafish as an animal model to understand the effect of vascular development in GB9 treated embryo. The results showed that GB9 treated embryos impair ISV (intersegmental vessel) growth, and CVP (caudal vein plexus) sprouting, which leads to pericardial edema and circulation defects. TUNEL assay and AO staining showed that vascular defects were not caused by apoptosis, but likely due to the impairment of cell proliferation and/or migration. We further showed GB9 treatment reduce cell proliferation marker p-HH3 and protein level, by counting p-HH3 immunostaining cell and Western blotting. In addition, we quantitated the migration distance of ISV between 24hpf and 28hpf and perform wound healing assay in endothelial cells EA.hy926. We show that GB9 treatment inhibits cell migration. Next, we examined the molecular mechanism of vascular defects by in situ hybridization, qPCR and Western blot, the results showed GB9 treatment decreases the expression of arteriovenous markers, ephrinb2, flt4, mrc1, flk and stabilinï¼the protein levels related to VEGF, BMP signal pathways. Those results suggest that GB9 interfere vascular signaling regulation in
zebrafish. Besides, we examine whether GB9 treatment cause vascular defect due to the increase of oxidative stress? We co-treated low-dose GB9 and H2O2, observed that defects in CVP formation was more severe than single treatment. Moreover, antioxidants Acetyl-cysteine (NAC) can rescue vascular defects in GB9 treated embryos. These data suggested that GB9 exposure causes vascular defects mediated by an increase in oxidative. Together our data show that GB9 treatment affect vascular development mediated by the interference of VEGF and BMP signals by the enhance of oxidative stress.
Advisors/Committee Members: Tzu-Fun Fu (chair), Zhi-Hong Wen (chair), Yung-Jen Chuang (chair), Chang-Yi Wu (committee member), ming-hong Tai (chair).
Subjects/Keywords: zebrafish; marine compound; angiogenesis; GB9
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❌
APA ·
Chicago ·
MLA ·
Vancouver ·
CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Song , Y. (2017). Coral-derived natural marine compound GB9 inhibits vascular development in zebrafish. (Thesis). NSYSU. Retrieved from http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0716117-115842
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Song , Yi-Chun. “Coral-derived natural marine compound GB9 inhibits vascular development in zebrafish.” 2017. Thesis, NSYSU. Accessed March 01, 2021.
http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0716117-115842.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Song , Yi-Chun. “Coral-derived natural marine compound GB9 inhibits vascular development in zebrafish.” 2017. Web. 01 Mar 2021.
Vancouver:
Song Y. Coral-derived natural marine compound GB9 inhibits vascular development in zebrafish. [Internet] [Thesis]. NSYSU; 2017. [cited 2021 Mar 01].
Available from: http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0716117-115842.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Song Y. Coral-derived natural marine compound GB9 inhibits vascular development in zebrafish. [Thesis]. NSYSU; 2017. Available from: http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0716117-115842
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

NSYSU
26.
Tu, Chia-Wen.
Effects of exercise hormone irisin in glucocorticoid-induced osteoporosis.
Degree: Master, Biological Sciences, 2017, NSYSU
URL: http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0723117-220830
► Regular exercise stimulated the expression of the Fibronectin type III domain-containing protein 5 (FNDC5) in skeletal muscle. In post-translation modification process, FNDC5 cleavage to a…
(more)
▼ Regular exercise stimulated the expression of the Fibronectin type III domain-containing protein 5 (FNDC5) in skeletal muscle. In post-translation modification process, FNDC5 cleavage to a new hormone: Irisin. Through released into blood circulation, Irisin help to improve obesity and stable blood sugar and menopause chronic complications. Base on previous studies, the IrisinR75E mutant impair Irisin dimerization through interfere salt bridge formation, it also leading to the functional deficiency in Irisin. Prednisolone, one kind of Glucocorticoids (GCs) drugs, that widely used in inflammatory and autoimmune conditions (allergy) treatment, however, it induced osteoporosis. Glucocorticoid-induced osteoporosis (GIOP) is the most common cause of secondary osteoporosis, the incidence rate was second to menopause-related osteoporosis. In this study, we have explored whether Irisin and IrisinR75E could stimulate osteoblast proliferation and differentiation in vitro, and clarified the Irisin and IrisinR75E in GIOP prevention effect in vivo. To discuss the proliferation effect, âPrednisoloneâ was added to the hFOB1.19 osteoblast cells, and cell viability was measured 24 hours post-treatment; In
zebrafish osteoporosis model, zebrafishes were cultured and immersed in Prednisolone (50 µM) for 2 weeks. The results indicated that Irisin stimulated osteoblast proliferation, but not IrisinR75E. Prednisolone inhibited osteoblast growth, but this phenomenon could be reversed by Irisin through upregulation of ALP activity and downregulation of TRAP activity. The conclusion represents that irisin enhances of osteoblast proliferation, bone density and improve osteoporosis. We suggest that Irisin could be used as a therapeutic agent for GIOP, besides, the established
zebrafish platform able to apply for human osteoporosis studies.
Advisors/Committee Members: Ming-Hong Tai (committee member), Wu, Chang-Yi (chair), Lee, Che-Hsin (chair).
Subjects/Keywords: GIOP; IrisinR75E; Irisin; Zebrafish
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❌
APA ·
Chicago ·
MLA ·
Vancouver ·
CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Tu, C. (2017). Effects of exercise hormone irisin in glucocorticoid-induced osteoporosis. (Thesis). NSYSU. Retrieved from http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0723117-220830
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Tu, Chia-Wen. “Effects of exercise hormone irisin in glucocorticoid-induced osteoporosis.” 2017. Thesis, NSYSU. Accessed March 01, 2021.
http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0723117-220830.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Tu, Chia-Wen. “Effects of exercise hormone irisin in glucocorticoid-induced osteoporosis.” 2017. Web. 01 Mar 2021.
Vancouver:
Tu C. Effects of exercise hormone irisin in glucocorticoid-induced osteoporosis. [Internet] [Thesis]. NSYSU; 2017. [cited 2021 Mar 01].
Available from: http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0723117-220830.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Tu C. Effects of exercise hormone irisin in glucocorticoid-induced osteoporosis. [Thesis]. NSYSU; 2017. Available from: http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0723117-220830
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
27.
Bournele, Despina.
Μελέτη των μηχανισμών εκδήλωσης συγγενών καρδιοπαθειών με την χρήση του zebrafish ως πειραματικό μοντέλο.
Degree: 2016, National and Kapodistrian University of Athens; Εθνικό και Καποδιστριακό Πανεπιστήμιο Αθηνών (ΕΚΠΑ)
URL: http://hdl.handle.net/10442/hedi/38345
► Cardiovascular disease (CVD) is a group of disorders of the heart and blood vessels and one of the leading causes of death worldwide. The most…
(more)
▼ Cardiovascular disease (CVD) is a group of disorders of the heart and blood vessels and one of the leading causes of death worldwide. The most significant risk factors associated with the development of heart diseases include genetic and environmental factors such as hypertension, high blood cholesterol levels, diabetes, smoking, and obesity. Coronary artery disease and stroke account for the highest percentage of CVD deaths and stroke, cardiomyopathies, congenital heart diseases, heart valve defects and arrhythmias follow. The causes, prevention, and treatment of all forms of cardiovascular disease remain active fields of biomedical research, with hundreds of scientific studies published on a weekly basis.Cardiac valves develop as the heart contracts, and they function throughout the lifetime of the organism to prevent retrograde blood flow. Their precise morphogenesis is crucial for cardiac function and depends on the interaction between cardiac contractility and intracardiac flow dynamics. Multiple signaling pathways are implicated in the morphogenesis of cardiac valves but little is known about the molecular mechanisms that orchestrate their formation.Several unique characteristics make zebrafish an attractive model for elucidating the mechanisms underlying cardiac valve morphogeneis. Non-invasive imaging allows in vivo analyses of cardiovascular phenotypes. In addition, zebrafish hearts maintain their ability to regenerate throughout their lifetime, providing novel insights to understand human cardiac regeneration. Zebrafish has also emerged as a high-throughput but low-cost model organism that combines the advantages of forward and reverse genetics with phenotype-driven drug screenings. Functional verification of candidate genes from Genome Wide Association Studies (GWAS) has verified the role of several genes in the pathophysiology of CVDs. Zebrafish is a valuable tool for the identification of candidate genes for congenital heart defects, either through mutant characterization from genetic screens or functional annotation through morphant analysis.Here we describe the identification and the characterization of genes involved in cardiac valve formation. The mutant lines that were used have derived from a forward mutagenesis genetic screen that was carried out in order to identify mutant phenotypes relevant to various aspects of cardiac development. Bua mutant embryos show an outflow tract stenosis phenotype, which results to abnormal atrioventricular valve formation and retrograde blood flow. BH mutants have smaller heart, body axis and head compared to wild-type embryos and have also curved tail.In parallel, we carried out the characterization of a new transgenic line for the wnt/b-catenin signaling pathway. Apc mutant embryos which show constitutive active wnt/b-catenin signaling have also been used for the elaborate characterization of the Tg(7xTCF-Xla.Siam:nlsmCherry) transgenic line.The goal of this research project was the study of gene function, the identification of new signaling pathways and the…
Subjects/Keywords: Συγγενείς καρδιοπάθειες; Cardiovascular disease; Zebrafish
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❌
APA ·
Chicago ·
MLA ·
Vancouver ·
CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Bournele, D. (2016). Μελέτη των μηχανισμών εκδήλωσης συγγενών καρδιοπαθειών με την χρήση του zebrafish ως πειραματικό μοντέλο. (Thesis). National and Kapodistrian University of Athens; Εθνικό και Καποδιστριακό Πανεπιστήμιο Αθηνών (ΕΚΠΑ). Retrieved from http://hdl.handle.net/10442/hedi/38345
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Bournele, Despina. “Μελέτη των μηχανισμών εκδήλωσης συγγενών καρδιοπαθειών με την χρήση του zebrafish ως πειραματικό μοντέλο.” 2016. Thesis, National and Kapodistrian University of Athens; Εθνικό και Καποδιστριακό Πανεπιστήμιο Αθηνών (ΕΚΠΑ). Accessed March 01, 2021.
http://hdl.handle.net/10442/hedi/38345.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Bournele, Despina. “Μελέτη των μηχανισμών εκδήλωσης συγγενών καρδιοπαθειών με την χρήση του zebrafish ως πειραματικό μοντέλο.” 2016. Web. 01 Mar 2021.
Vancouver:
Bournele D. Μελέτη των μηχανισμών εκδήλωσης συγγενών καρδιοπαθειών με την χρήση του zebrafish ως πειραματικό μοντέλο. [Internet] [Thesis]. National and Kapodistrian University of Athens; Εθνικό και Καποδιστριακό Πανεπιστήμιο Αθηνών (ΕΚΠΑ); 2016. [cited 2021 Mar 01].
Available from: http://hdl.handle.net/10442/hedi/38345.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Bournele D. Μελέτη των μηχανισμών εκδήλωσης συγγενών καρδιοπαθειών με την χρήση του zebrafish ως πειραματικό μοντέλο. [Thesis]. National and Kapodistrian University of Athens; Εθνικό και Καποδιστριακό Πανεπιστήμιο Αθηνών (ΕΚΠΑ); 2016. Available from: http://hdl.handle.net/10442/hedi/38345
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Manchester
28.
Haud, Noemie Magali Renee.
A forward genetic screen to identify modifiers of
chemotherapy using zebrafish- Study of rnaset2 deficiency in
zebrafish.
Degree: 2010, University of Manchester
URL: http://www.manchester.ac.uk/escholar/uk-ac-man-scw:90343
► Chemotherapy frequently fails to cure cancer patients due to toxicity or resistance to treatment. Variability in toxicity and resistance is influenced by polymorphisms and mutations…
(more)
▼ Chemotherapy frequently fails to cure cancer
patients due to toxicity or resistance to treatment. Variability in
toxicity and resistance is influenced by polymorphisms and
mutations in the individual's genome (Pharmacogenetics).
Zebrafish
has extensive evolutionary conservation with human, its genetics is
a powerful gene discovery tool, and it has been described as a
suitable model in cancer research. To study chemotherapy
resistance, we used ENU-mutagenised
zebrafish in a forward genetic
screen to identify genes that modify responses to cancer
chemotherapy.
Zebrafish larvae were challenged with two
chemotherapeutic drugs and stained with acridine orange (AO) to
detect apoptosis and reveal hypo- or hyper-responders to
chemotherapy. A mutation, conferring an increased uptake of AO, was
identified by genetic mapping as a premature stop codon truncating
the ribonuclease T2 (rnaset2) gene. Human RNASET2 encodes a
putative lysosomal RNase. Lysosomal storage disorders, due to
deficiencies in lysosomal hydrolases and resultant accumulation of
macromolecules within lysosomes, are collectively among the
commonest genetic diseases. RNASET2 deficiency in man results in a
static encephalopathy arising in infancy and characterized by
multifocal bilateral white matter lesions, subcortical cysts and
focal enlargement of the anterior inferior horn. This doctoral
thesis demonstrates that rnaset2 deficient
zebrafish embryos suffer
from a lysosomal storage disorder accumulating undigested ribosomal
RNA (rRNA) in enlarged lysosomes within neurons of the brain.
Moreover, high-field intensity μMRI revealed white matter lesions
in the brain of adult rnase2 mutant animals. Thus, this
zebrafish
mutant can be used as a model to study the abnormalities observed
in RNASET2 deficient individuals. This model suggests that the
leukoencephalopathy results from a lysosomal storage disorder and
provides a preclinical model for further elaborating disease
mechanisms and evaluating candidate therapeutics.RNASET2 has also
been advanced as a candidate tumour suppressor in several solid
tumours. Recombinant rnaset2 protein has been tested in the clinic
as an anti-cancer cytotoxic agent, with anti-angiogenic properties.
By combining the rnaset2 mutant presented here with a transgenic
melanoma model developed in the laboratory, the tumour suppressor
and angiogenic role for rnaset2 was refuted.
Advisors/Committee Members: Hurlstone, Adam.
Subjects/Keywords: lysosomes; zebrafish
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❌
APA ·
Chicago ·
MLA ·
Vancouver ·
CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Haud, N. M. R. (2010). A forward genetic screen to identify modifiers of
chemotherapy using zebrafish- Study of rnaset2 deficiency in
zebrafish. (Doctoral Dissertation). University of Manchester. Retrieved from http://www.manchester.ac.uk/escholar/uk-ac-man-scw:90343
Chicago Manual of Style (16th Edition):
Haud, Noemie Magali Renee. “A forward genetic screen to identify modifiers of
chemotherapy using zebrafish- Study of rnaset2 deficiency in
zebrafish.” 2010. Doctoral Dissertation, University of Manchester. Accessed March 01, 2021.
http://www.manchester.ac.uk/escholar/uk-ac-man-scw:90343.
MLA Handbook (7th Edition):
Haud, Noemie Magali Renee. “A forward genetic screen to identify modifiers of
chemotherapy using zebrafish- Study of rnaset2 deficiency in
zebrafish.” 2010. Web. 01 Mar 2021.
Vancouver:
Haud NMR. A forward genetic screen to identify modifiers of
chemotherapy using zebrafish- Study of rnaset2 deficiency in
zebrafish. [Internet] [Doctoral dissertation]. University of Manchester; 2010. [cited 2021 Mar 01].
Available from: http://www.manchester.ac.uk/escholar/uk-ac-man-scw:90343.
Council of Science Editors:
Haud NMR. A forward genetic screen to identify modifiers of
chemotherapy using zebrafish- Study of rnaset2 deficiency in
zebrafish. [Doctoral Dissertation]. University of Manchester; 2010. Available from: http://www.manchester.ac.uk/escholar/uk-ac-man-scw:90343

Vanderbilt University
29.
Li, Nan.
MiRNA function during early vertebrate development.
Degree: PhD, Biological Sciences, 2010, Vanderbilt University
URL: http://hdl.handle.net/1803/12868
► microRNAs (miRNAs) are short non-coding RNAs that down-regulate gene expression by pairing with sequences in the 3’UTR of target mRNAs. They play critical roles in…
(more)
▼ microRNAs (miRNAs) are short non-coding RNAs that down-regulate gene expression by pairing with sequences in the 3’UTR of target mRNAs. They play critical roles in diverse developmental and physiological events but the exact function of most miRNAs remains to be elucidated. Identification of bona fide targets is challenging due to partial base pairing between miRNAs and target 3’UTRs. To understand miRNA function during early vertebrate development, I first analyzed the general rules for miRNA:mRNA base pairing. By comparing the silencing ability of different miRNA recognition sites, I show that combinations of weak binding sites are just as effective as perfect sites for miRNA function. In addition, I uncovered a novel tissue-inductive role for miR-92 during early
zebrafish development. Most previous work has focused on control of a number of cancer-related genes by miR-92, but here, using gain-of-function, loss-of-function, target identification, and genetic epistasis experiments, I demonstrate that miR-92 also regulates transcription factor gata5 and that such regulation is essential for proper endoderm formation and left-right patterning in
zebrafish.
Advisors/Committee Members: Ronald B Emeson (committee member), Joshua T Gamse (committee member), Todd R Graham (committee member), Lilianna Solnica-Krezel (committee member), James G Patton (Committee Chair).
Subjects/Keywords: zebrafish; microRNA
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❌
APA ·
Chicago ·
MLA ·
Vancouver ·
CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Li, N. (2010). MiRNA function during early vertebrate development. (Doctoral Dissertation). Vanderbilt University. Retrieved from http://hdl.handle.net/1803/12868
Chicago Manual of Style (16th Edition):
Li, Nan. “MiRNA function during early vertebrate development.” 2010. Doctoral Dissertation, Vanderbilt University. Accessed March 01, 2021.
http://hdl.handle.net/1803/12868.
MLA Handbook (7th Edition):
Li, Nan. “MiRNA function during early vertebrate development.” 2010. Web. 01 Mar 2021.
Vancouver:
Li N. MiRNA function during early vertebrate development. [Internet] [Doctoral dissertation]. Vanderbilt University; 2010. [cited 2021 Mar 01].
Available from: http://hdl.handle.net/1803/12868.
Council of Science Editors:
Li N. MiRNA function during early vertebrate development. [Doctoral Dissertation]. Vanderbilt University; 2010. Available from: http://hdl.handle.net/1803/12868

Vanderbilt University
30.
Carlin, Daniel L.
The homeodomain transcription factor Six3 is required for telencephalon patterning in zebrafish.
Degree: PhD, Biological Sciences, 2012, Vanderbilt University
URL: http://hdl.handle.net/1803/14113
► The adult vertebrate forebrain is responsible for a diverse set of behaviors, and as such exhibits complex anatomy. This complexity is generated during embryogenesis whereby…
(more)
▼ The adult vertebrate forebrain is responsible for a diverse set of behaviors, and as such exhibits complex anatomy. This complexity is generated during embryogenesis whereby a specific spatiotemporal sequence of transcriptional and signaling programs promotes specification of different cell types based on the location and developmental potential of progenitors. Six3 is one such transcription factor that exerts multiple functions in the development of anterior neural tissue of vertebrate embryos. Whereas complete loss of Six3 function in the mouse results in failure of forebrain formation, its hypomorphic mutations in human and mouse can promote holoprosencephaly, a forebrain malformation resulting, at least in part, from abnormal telencephalon development. However, Six3’s roles in telencephalon patterning and differentiation are not well understood. The
zebrafish genome contains three Six3-related genes facilitating analysis of different partial loss-of-function combinations. I analyzed
zebrafish embryos deficient in two of three Six3-related genes, six3b and six7, representing a partial loss of Six3 function. Telencephalon forms in six3b;six7-deficient embryos, however ventral telencephalic domains are reduced and dorsal domains are expanded. Decreased cell proliferation or excess apoptosis cannot account for the ventral deficiency. Instead, six3b and six7 are required during early segmentation for specification of ventral progenitors, similar to the role of Hedgehog signaling in telencephalon development. Unlike in mice, Hedgehog signaling is not disrupted in embryos with reduced Six3 function. Furthermore, six3b overexpression is sufficient to compensate for loss of Hedgehog signaling in isl1- but not nkx2.1b-positive cells, suggesting a novel Hedgehog-independent role for Six3 in telencephalon patterning. Additional investigations into the interactions between Six3 and known telencepnalon patterning genes showed that Six3 promotes ventral telencephalic fates through transient regulation of foxg1a expression and repression of Wnt/β-catenin pathway. As Six3-related genes are expressed broadly in prechordal mesoderm and anterior neuroectoderm, transgenic
zebrafish were generated to identify the spatial requirement for Six3 function in telencephalon patterning. My studies help define the cellular mechanisms of Six3-mediated dorsoventral patterning in telencephalon and present a novel genetic mechanism by which Six3 regulates this process.
Advisors/Committee Members: Joshua Gamse (committee member), Kenneth Catania (committee member), Chin Chiang (committee member), Lilianna Solnica-Krezel (committee member), Laurence Zwiebel (Committee Chair).
Subjects/Keywords: telencephalon; Foxg1; Six3; zebrafish
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❌
APA ·
Chicago ·
MLA ·
Vancouver ·
CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Carlin, D. L. (2012). The homeodomain transcription factor Six3 is required for telencephalon patterning in zebrafish. (Doctoral Dissertation). Vanderbilt University. Retrieved from http://hdl.handle.net/1803/14113
Chicago Manual of Style (16th Edition):
Carlin, Daniel L. “The homeodomain transcription factor Six3 is required for telencephalon patterning in zebrafish.” 2012. Doctoral Dissertation, Vanderbilt University. Accessed March 01, 2021.
http://hdl.handle.net/1803/14113.
MLA Handbook (7th Edition):
Carlin, Daniel L. “The homeodomain transcription factor Six3 is required for telencephalon patterning in zebrafish.” 2012. Web. 01 Mar 2021.
Vancouver:
Carlin DL. The homeodomain transcription factor Six3 is required for telencephalon patterning in zebrafish. [Internet] [Doctoral dissertation]. Vanderbilt University; 2012. [cited 2021 Mar 01].
Available from: http://hdl.handle.net/1803/14113.
Council of Science Editors:
Carlin DL. The homeodomain transcription factor Six3 is required for telencephalon patterning in zebrafish. [Doctoral Dissertation]. Vanderbilt University; 2012. Available from: http://hdl.handle.net/1803/14113
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