subject:(trophoblast cells)

University of Ottawa

1. Nguyen, Tina. Mode of Entry and Survival of Salmonella Enterica Serovar Typhimurium in Trophoblast Cells .

Degree: 2017, University of Ottawa

► Salmonella enterica species are intracellular bacteria causative agents of gastroenteritis and typhoid fever in humans. Pregnancy poses an increased risk of severe Salmonellosis in many… (more)

▼ Salmonella enterica species are intracellular bacteria causative agents of gastroenteritis and typhoid fever in humans. Pregnancy poses an increased risk of severe Salmonellosis in many mammalian species contributing to miscarriage and/or maternal illness. Previous studies indicated that Salmonella infection in pregnant mice caused rapid fetal and maternal death due to massive bacterial proliferation in the placenta. However, the susceptibility of human primary trophoblast cells (cTBCs) to Salmonella infection was not known. We hypothesized that human placental trophoblast cells are productively infected and provide a unique intracellular niche that permits uncontrolled Salmonella replication due to an ineffective maternal innate immune response to the virulent bacteria resulting in placental death. Firstly, we observed that S.Tm strains defective in the Salmonella pathogenicity island (SPI)-1 type III secretion system (TTSS) (S.Tm-ΔinvA) were unable to enter epithelial cells, but efficiently infected placental choriocarcinoma cell lines through scavenger receptor-mediated endocytosis. Next, we observed that S.Tm failed to grow vigorously in macrophages, but replicated rapidly within epithelial and placental trophoblast cells. Further examination of intracellular localization of S.Tm indicated that bacteria were arrested in early Rab5 expressing phagosomal vesicles within trophoblast cells, whereas phagosomal maturation progressed steadily in macrophages (with expression of lysosomal-associated membrane protein-1 (LAMP-1) and cathepsin D). Moreover, human primary cTBCs harboring S.Tm underwent rapid death of the cells. Infected cTBCs expressed phosphorylated-receptor-interacting serine/threonine-protein kinase (RIPK)-1 protein and phosphorylated-mixed lineage kinase domain-like (MLKL), suggesting induction of the necroptosis pathway of cell death. Furthermore, specific inhibition of necroptosis rescued S.Tm-induced death of cTBCs. Finally, S.Tm infected trophoblast cells produced interleukin (IL)-10, and signal transducer and activator of transcription (STAT)-3 signalling. This correlated to delayed phagosomal maturation which consequently facilitated intracellular pathogen proliferation. Overall, human trophoblast cells may act as reservoirs for S.Tm survival and may aid dissemination in the pregnant host.

Subjects/Keywords: Trophoblast cells; Infection; Pregnancy; Salmonella Typhimurium

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APA (6^th Edition):

Nguyen, T. (2017). Mode of Entry and Survival of Salmonella Enterica Serovar Typhimurium in Trophoblast Cells . (Thesis). University of Ottawa. Retrieved from http://hdl.handle.net/10393/36032

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Nguyen, Tina. “Mode of Entry and Survival of Salmonella Enterica Serovar Typhimurium in Trophoblast Cells .” 2017. Thesis, University of Ottawa. Accessed March 09, 2021. http://hdl.handle.net/10393/36032.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Nguyen, Tina. “Mode of Entry and Survival of Salmonella Enterica Serovar Typhimurium in Trophoblast Cells .” 2017. Web. 09 Mar 2021.

Vancouver:

Nguyen T. Mode of Entry and Survival of Salmonella Enterica Serovar Typhimurium in Trophoblast Cells . [Internet] [Thesis]. University of Ottawa; 2017. [cited 2021 Mar 09]. Available from: http://hdl.handle.net/10393/36032.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Nguyen T. Mode of Entry and Survival of Salmonella Enterica Serovar Typhimurium in Trophoblast Cells . [Thesis]. University of Ottawa; 2017. Available from: http://hdl.handle.net/10393/36032

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of California – San Diego

2. Arul Nambi Rajan, Kanaga Sundari Rajam. Sirt1 in Trophoblast Differentiation and Placental Development.

Degree: Biomedical Sciences, 2016, University of California – San Diego

URL: http://www.escholarship.org/uc/item/98s8g5kf

► The placenta is a fetal-derived transient organ critical for pregnancy and fetal development. During gestation, this organ is responsible for implantation into the maternal uterus… (more)

▼ The placenta is a fetal-derived transient organ critical for pregnancy and fetal development. During gestation, this organ is responsible for implantation into the maternal uterus as well as facilitating gas and nutrient exchange between the mother and fetus. Defects in placental development result in pregnancy complications such as preeclampsia and intrauterine growth restriction (IUGR). The damage done by these defects can last beyond pregnancy. Not only will placental defects compromise the health of mother and fetus during gestation but will also predispose infants to adult-onset diseases such as diabetes, obesity, and cardiovascular disease.Sirtuin1 (SIRT1) is a NAD-dependent protein deacetylase expressed in an array of tissues and implicated in many different disease models. It regulates a variety of cellular processes such as apoptosis, inflammation, and differentiation, through deacetylation of specific proteins. Sirt1 is considered a key metabolic sensor and is therefore often implicated in metabolic diseases such as diabetes and obesity. Homozygous Sirt1-null mice have previously been reported to be growth restricted. However, though Sirt1 has been implicated in pregnancy, it has never been studied specifically in placental development and trophoblast differentiation.For my dissertation, I explored the role of Sirt1 in mouse trophoblast differentiation and placental development. Using Sirt1-null mice, I found that in the absence of Sirt1, embryos die at e13.5 and placentas are consistently smaller. Upon investigation of the null placentas, I observed defects in both the labyrinthine layer and the junctional zone. Differentiation of wild-type and Sirt1-null trophoblast stem (TS) cells showed that, in the absence of Sirt1, TS cells fail to terminally differentiate and instead are halted in an Epcam+ labyrinthine trophoblast progenitor state. This defect is due, at least in part, to dysregulation of c-Met signaling. These findings elucidate a new role for Sirt1 during trophoblast differentiation, and indicate a potential pathway through which abnormal placental development can contribute to fetal growth restriction.

Subjects/Keywords: Developmental biology; Molecular biology; Cellular biology; Differentiation; Placenta; Sirt1; Trophoblast; Trophoblast stem cells

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APA (6^th Edition):

Arul Nambi Rajan, K. S. R. (2016). Sirt1 in Trophoblast Differentiation and Placental Development. (Thesis). University of California – San Diego. Retrieved from http://www.escholarship.org/uc/item/98s8g5kf

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Arul Nambi Rajan, Kanaga Sundari Rajam. “Sirt1 in Trophoblast Differentiation and Placental Development.” 2016. Thesis, University of California – San Diego. Accessed March 09, 2021. http://www.escholarship.org/uc/item/98s8g5kf.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Arul Nambi Rajan, Kanaga Sundari Rajam. “Sirt1 in Trophoblast Differentiation and Placental Development.” 2016. Web. 09 Mar 2021.

Vancouver:

Arul Nambi Rajan KSR. Sirt1 in Trophoblast Differentiation and Placental Development. [Internet] [Thesis]. University of California – San Diego; 2016. [cited 2021 Mar 09]. Available from: http://www.escholarship.org/uc/item/98s8g5kf.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Arul Nambi Rajan KSR. Sirt1 in Trophoblast Differentiation and Placental Development. [Thesis]. University of California – San Diego; 2016. Available from: http://www.escholarship.org/uc/item/98s8g5kf

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University College Cork

3. Williams, John M. The expression and regulation of pregnancy-specific glycoproteins in the mouse.

Degree: 2013, University College Cork

URL: http://hdl.handle.net/10468/1951

► Pregnancy-Specific Glycoproteins (PSG) are the most abundant fetally expressed proteins in the maternal bloodstream at term. This multigene family are immunoglobulin superfamily members and are… (more)

Subjects/Keywords: PSG; Placenta; Trophoblast stem cells; Giant cells; Pregenancy-specific glycoproteins

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APA (6^th Edition):

Williams, J. M. (2013). The expression and regulation of pregnancy-specific glycoproteins in the mouse. (Thesis). University College Cork. Retrieved from http://hdl.handle.net/10468/1951

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Williams, John M. “The expression and regulation of pregnancy-specific glycoproteins in the mouse.” 2013. Thesis, University College Cork. Accessed March 09, 2021. http://hdl.handle.net/10468/1951.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Williams, John M. “The expression and regulation of pregnancy-specific glycoproteins in the mouse.” 2013. Web. 09 Mar 2021.

Vancouver:

Williams JM. The expression and regulation of pregnancy-specific glycoproteins in the mouse. [Internet] [Thesis]. University College Cork; 2013. [cited 2021 Mar 09]. Available from: http://hdl.handle.net/10468/1951.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Williams JM. The expression and regulation of pregnancy-specific glycoproteins in the mouse. [Thesis]. University College Cork; 2013. Available from: http://hdl.handle.net/10468/1951

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Texas – Austin

4. Rhee, Catherine Soo. Transcriptional and epigenetic mechanisms of the first cell fate decision and reprogramming.

Degree: PhD, Cell and Molecular Biology, 2016, University of Texas – Austin

URL: http://hdl.handle.net/2152/72695

► The placenta is a transient but vital organ mediating a myriad of interactions between maternal and embryonic tissues. The cells in the trophectoderm (TE) lineage… (more)

▼ The placenta is a transient but vital organ mediating a myriad of interactions between maternal and embryonic tissues. The cells in the trophectoderm (TE) lineage are responsible for proper implantation, placentation, and immunological functions of the placenta. However, our understanding of molecular mechanisms underlying placentation and TE development is still rudimentary. Deciphering the mechanisms by which key TE-specific transcription factors (TFs) control the first cell fate decision, as well as the maintenance and differentiation of TE, is a prerequisite for understanding early embryonic development and ultimately improving healthy pregnancy. First, using a combination of functional genomics, bioinformatics, and mouse genetics, I revealed that Arid3a is a critical regulator for controlling the first cell fate decision and placental development. Ectopically expressed Arid3a induces TE-like gene expression programs in embryonic stem (ES) cells. Moreover, Arid3a is not only essential for maintaining self-renewing TS cells, but also for promoting further differentiation of trophoblastic lineages. Consistently, Arid3a-/- mice suffer from severely impaired post-implantation development, resulting in early embryonic lethality. I further showed that Arid3a directly activates TE-specific genes while repressing pluripotency genes by recruiting HDAC1. Second, I studied the mechanisms underlying TF-mediated conversion of ES to trophoblast stem (TS)-like cells. Upon overexpression of TS cell-specific TFs, Cdx2, Arid3a, and Gata3 (CAG factors) in ES cells, I performed time–course profiling of chromatin accessibility, transcriptomes, and occupancy of these reprogramming factors during reprogramming. Using an integrative analysis, I discovered that CAG factors orchestrate the conversion via a sequential two-step regulation in a timely, ordered manner, with repression of pluripotency genes by decommissioning active enhancers, followed by activation of TS cell-specific genes as pioneer factors that can access closed chromatin. Taken together, my studies unveiled that Arid3a functions as a pivotal regulator of TE and placental development by regulating the commitment to the first cell fate, as well as by executing TE lineage differentiation. I advanced our understanding of the mechanisms underlying TF-mediated reprogramming of ES to TS-like cells, in particular Arid3a-mediated transcriptional and epigenetic regulation. Thus, my studies will be beneficial for enhancing clinical applications such as disease modeling, drug screening, and regenerative therapies. Advisors/Committee Members: Kim, Jonghwan, 1971- (advisor), Tucker, Haley O. (advisor), Iyer, Vishwanath R (committee member), Vokes, Steven A (committee member), Marcotte, Edward M (committee member).

Subjects/Keywords: Arid3a; Reprogramming; Embryonic stem cells; Trophoblast stem cells

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Rhee, C. S. (2016). Transcriptional and epigenetic mechanisms of the first cell fate decision and reprogramming. (Doctoral Dissertation). University of Texas – Austin. Retrieved from http://hdl.handle.net/2152/72695

Chicago Manual of Style (16^th Edition):

Rhee, Catherine Soo. “Transcriptional and epigenetic mechanisms of the first cell fate decision and reprogramming.” 2016. Doctoral Dissertation, University of Texas – Austin. Accessed March 09, 2021. http://hdl.handle.net/2152/72695.

MLA Handbook (7^th Edition):

Rhee, Catherine Soo. “Transcriptional and epigenetic mechanisms of the first cell fate decision and reprogramming.” 2016. Web. 09 Mar 2021.

Vancouver:

Rhee CS. Transcriptional and epigenetic mechanisms of the first cell fate decision and reprogramming. [Internet] [Doctoral dissertation]. University of Texas – Austin; 2016. [cited 2021 Mar 09]. Available from: http://hdl.handle.net/2152/72695.

Council of Science Editors:

Rhee CS. Transcriptional and epigenetic mechanisms of the first cell fate decision and reprogramming. [Doctoral Dissertation]. University of Texas – Austin; 2016. Available from: http://hdl.handle.net/2152/72695

University of Alberta

5. Riddell, Meghan R. Mechanisms of Human Placental Growth.

Degree: PhD, Department of Physiology, 2013, University of Alberta

URL: https://era.library.ualberta.ca/files/pc289j734

► The placenta is an essential transitory fetal organ responsible for the key processes of nutrient, oxygen, and waste transfer between the mother and the fetus… (more)

▼ The placenta is an essential transitory fetal organ responsible for the key processes of nutrient, oxygen, and waste transfer between the mother and the fetus throughout gestation. Placental size is, importantly, known to correlate to fetal weight, and the malfunction and malformation of the placenta is associated with the common pregnancy complication intrauterine growth restriction (IUGR). IUGR affects ~10% of all pregnancies is defined as a fetus that fails to achieve its genetic growth potential. No curative therapies are currently available for IUGR and a potential target for the development of treatments would be to increase placental growth. Thus, this thesis focused on elucidating mechanisms of two key processes for placental growth: 1) differentiation of the syncytiotrophoblastic epithelium, and 2) extension of the placental blood vessels, or angiogenesis. Trophoblast differentiation is an essential process in placental growth for the syncytiotrophoblast is a single giant, multinucleate cell, covering the entire surface of the placenta and it is maintained and expands only through differentiation. Previous publications have presented evidence that the initiation of the apoptotic cascade and the externalization of the membrane phospholipid, phosphatidylserine, are required for trophoblast differentiation. However, many of these studies are controversial and were conducted in cell lines. The studies presented in this thesis demonstrate with primary cells that both apoptosis and the externalization of phosphatidylserine have no role in trophoblast differentiation. The remaining studies present the identification of a novel population of fibroblastic cells within the human placenta, fibrocyte-like cells, and the ability of these cells to induce placental angiogenesis in vitro. It is also demonstrated that fibrocyte-like cells from IUGR placentas have a reduced ability to stimulate in vitro angiogenesis and thus may contribute to the malformation of the placenta in the established condition. In conclusion, the results presented in this thesis are an important contribution to the understanding of the mechanisms of trophoblast differentiation and placental angiogenesis therefore significantly contributing to our understanding of placental growth.

Subjects/Keywords: trophoblast differentiation; Placenta; Intrauterine growth restriction; angiogenesis; stromal cells

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APA (6^th Edition):

Riddell, M. R. (2013). Mechanisms of Human Placental Growth. (Doctoral Dissertation). University of Alberta. Retrieved from https://era.library.ualberta.ca/files/pc289j734

Chicago Manual of Style (16^th Edition):

Riddell, Meghan R. “Mechanisms of Human Placental Growth.” 2013. Doctoral Dissertation, University of Alberta. Accessed March 09, 2021. https://era.library.ualberta.ca/files/pc289j734.

MLA Handbook (7^th Edition):

Riddell, Meghan R. “Mechanisms of Human Placental Growth.” 2013. Web. 09 Mar 2021.

Vancouver:

Riddell MR. Mechanisms of Human Placental Growth. [Internet] [Doctoral dissertation]. University of Alberta; 2013. [cited 2021 Mar 09]. Available from: https://era.library.ualberta.ca/files/pc289j734.

Council of Science Editors:

Riddell MR. Mechanisms of Human Placental Growth. [Doctoral Dissertation]. University of Alberta; 2013. Available from: https://era.library.ualberta.ca/files/pc289j734

Wayne State University

6. Fritz, Rani. Trophoblast Retrieval And Isolation From The Cervix (tric) For Non-Invasive Prenatal Genetic Diagnosis And Prediction Of Abnormal Pregnancy Outcome.

Degree: PhD, Physiology, 2015, Wayne State University

URL: https://digitalcommons.wayne.edu/oa_dissertations/1310

► The placenta is vital for the short- and long-term health of the fetus, and significantly impacts the health of the mother. During the first… (more)

▼ The placenta is vital for the short- and long-term health of the fetus, and significantly impacts the health of the mother. During the first and second trimesters of pregnancy, extravillous trophoblast (EVT) cells invade the uterus and remodel the maternal spiral arteries, which, if inadequate, leads to pregnancy complications, including early pregnancy loss (EPL), preeclampsia (PE), and intra-uterine growth restriction (IUGR). EVT migration into the uterine wall is dependent on growth factors and cytokines that signal between maternal and fetal tissues. The epidermal growth factor (EGF) signaling system plays a significant role in trophoblast function. Using immunocytochemistry (ICC), we evaluated EGF family growth factors in post-partum PE placentas (villi and basal plate), and maternal serum, comparing gestational age (GA)-matched adverse preterm pregnancies and uncomplicated term pregnancies. EGF, heparin-binding EGF-like growth factor (HBEGF), and transforming growth factor alpha (TGFA) were significantly decreased in placentas from PE compared to IUGR, preterm labor, and uncomplicated term placentas. It was uncertain whether reduced growth factor signaling contributed to disease, or was the result of late-stage pathology. However, EGF was significantly reduced in prenatal serum of pregnant patients with PE compared to GA-matched patients without PE, demonstrating that the EGF signaling system is disrupted before PE symptoms dictate delivery. Currently, chorionic villous sampling is the only means by which intact placental cells can be obtained from an ongoing pregnancy. However, the earliest this invasive procedure can be preformed is 10 weeks GA, and it carries a risk of fetal loss. Trophoblast retrieval and isolation from the cervix (TRIC) can isolate intact trophoblast cells in a minimally invasive procedure from ongoing pregnancies as early as 5 weeks GA, using immunomagnetic isolation to target human leukocyte antigen-G, a protein present on trophoblast cells, but not on maternal cervical cells. ICC shows that the isolated cells are of the extravillous phenotype. The ability to isolate EVT cells from ongoing pregnancies not only provides clinicians with a tool to study the placenta in real time, but is also of benefit for prenatal genetic diagnosis (PGD). EVT protein expression was investigated, using TRIC with patient specimens obtained between 5-20 weeks GA. Using ICC, galectin 13 (LGALS13), galectin 14 (LGALS14), pregnancy-associated plasma protein-A (PAPPA), placental growth factor (PGF), endoglin (ENG), fms-like tyrosine kinase-1 (FLT1), and alpha-fetoprotein (AFP), proteins known to play a role in EVT function, were evaluated in EVT cells from patients that developed PE, IUGR, or EPL, and compared to pregnancies with uncomplicated term deliveries. Expression of LGALS14, PAPPA, and PGF significantly decreased in EVT cells from patients that developed IUGR, PE, or EPL, whereas expression of ENG, FLT1, and AFP were all significantly increased. To evaluate the… Advisors/Committee Members: D. Randall Armant.

Subjects/Keywords: Early Pregnancy Loss; Extravillous trophoblast cells; Placenta; Preeclampsia; Prenatal Diagnosis; Physiology

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APA (6^th Edition):

Fritz, R. (2015). Trophoblast Retrieval And Isolation From The Cervix (tric) For Non-Invasive Prenatal Genetic Diagnosis And Prediction Of Abnormal Pregnancy Outcome. (Doctoral Dissertation). Wayne State University. Retrieved from https://digitalcommons.wayne.edu/oa_dissertations/1310

Chicago Manual of Style (16^th Edition):

Fritz, Rani. “Trophoblast Retrieval And Isolation From The Cervix (tric) For Non-Invasive Prenatal Genetic Diagnosis And Prediction Of Abnormal Pregnancy Outcome.” 2015. Doctoral Dissertation, Wayne State University. Accessed March 09, 2021. https://digitalcommons.wayne.edu/oa_dissertations/1310.

MLA Handbook (7^th Edition):

Fritz, Rani. “Trophoblast Retrieval And Isolation From The Cervix (tric) For Non-Invasive Prenatal Genetic Diagnosis And Prediction Of Abnormal Pregnancy Outcome.” 2015. Web. 09 Mar 2021.

Vancouver:

Fritz R. Trophoblast Retrieval And Isolation From The Cervix (tric) For Non-Invasive Prenatal Genetic Diagnosis And Prediction Of Abnormal Pregnancy Outcome. [Internet] [Doctoral dissertation]. Wayne State University; 2015. [cited 2021 Mar 09]. Available from: https://digitalcommons.wayne.edu/oa_dissertations/1310.

Council of Science Editors:

Fritz R. Trophoblast Retrieval And Isolation From The Cervix (tric) For Non-Invasive Prenatal Genetic Diagnosis And Prediction Of Abnormal Pregnancy Outcome. [Doctoral Dissertation]. Wayne State University; 2015. Available from: https://digitalcommons.wayne.edu/oa_dissertations/1310

University of Toronto

7. Nosi, Ursula. Investigating the Role of microRNAs in Cell Fate Determination.

Degree: PhD, 2019, University of Toronto

URL: http://hdl.handle.net/1807/101747

►

miRNAs are key regulators of a myriad of cellular processes, including proliferation, differentiation, and signaling. These developmentally regulated and abundantly expressed RNA species elicit their… (more)

Subjects/Keywords: Embryo; Embryonic Stem Cells; Genetics; microRNA; Trophoblast; 0758

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APA (6^th Edition):

Nosi, U. (2019). Investigating the Role of microRNAs in Cell Fate Determination. (Doctoral Dissertation). University of Toronto. Retrieved from http://hdl.handle.net/1807/101747

Chicago Manual of Style (16^th Edition):

Nosi, Ursula. “Investigating the Role of microRNAs in Cell Fate Determination.” 2019. Doctoral Dissertation, University of Toronto. Accessed March 09, 2021. http://hdl.handle.net/1807/101747.

MLA Handbook (7^th Edition):

Nosi, Ursula. “Investigating the Role of microRNAs in Cell Fate Determination.” 2019. Web. 09 Mar 2021.

Vancouver:

Nosi U. Investigating the Role of microRNAs in Cell Fate Determination. [Internet] [Doctoral dissertation]. University of Toronto; 2019. [cited 2021 Mar 09]. Available from: http://hdl.handle.net/1807/101747.

Council of Science Editors:

Nosi U. Investigating the Role of microRNAs in Cell Fate Determination. [Doctoral Dissertation]. University of Toronto; 2019. Available from: http://hdl.handle.net/1807/101747

8. Shen, Qian. Uncovering new functions for histone variants: a role for H2A.Z in silencing retrotransposons .

Degree: 2016, Australian National University

URL: http://hdl.handle.net/1885/104595

► Eukaryotic genomes must be, on the one hand, highly compacted by wrapping their DNA around histones to form nucleosomes while on the other, still remain… (more)

▼ Eukaryotic genomes must be, on the one hand, highly compacted by wrapping their DNA around histones to form nucleosomes while on the other, still remain accessible to the transcriptional machinery in a developmental and cell-type specific manner. Various epigenetic-based mechanisms exist that can regulate DNA accessibility ensuring the proper regulation of gene transcription. One epigenetic modification in particular that impacts all aspects of genome function and organization is the replacement of canonical histones with their variant forms. Among the histone variants that are most extensively studied is the essential and evolutionary conserved variant, H2A.Z. Recent studies have revealed many layers of regulation and complex functions of H2A.Z in modulating gene expression. Notably, H2A.Z has the ability to both activate and repress transcription but it remains unresolved how H2A.Z affects gene expression in these opposing ways. At a genome-wide level, H2A.Z is found at active transcription start sites, inactive promoters and of relevance to this thesis, it is also found at satellite repeats including those present at constitutive heterochromatin and the centromere. To gain new insights into the function of H2A.Z and how it can perform these opposing roles, this thesis has examined the transcriptional role of H2A.Z in regulating two different types of RNAPII transcribed elements: the retrotransposon, LINE-1 and a coding gene important for T-cell development, Cd69. Long Interspersed Nucleotide Element-1 (LINE-1) is one of most impactful and still active transposable elements (TEs) that occupy 18%-20% of mammalian genomes. Given that H2A.Z is targeted to repetitive mouse DNA sequences found at heterochromatin and the centromere, which is necessary for chromatin compaction, I wondered whether this role might extend to other repetitive DNA elements. Specifically, I explored the role of H2A.Z in LINE-1 transcription in four different cell types that were undifferentiated (mouse trophoblast stem cells (TSCs)), differentiated (trophoblast giant cells (TGCs) and mouse L929 cells) and committed (mouse embryonic fibroblasts (MEFs)). The results showed significant variability in H2A.Z occupancy relative to H2A at LINE-1s in these different cell types. Moreover, the enrichment of H2A.Z was inversely correlated with LINE-1 RNA expression. Specifically, relative high levels of LINE-1 transcripts were observed in TSCs compared to MEFs and L929 cells and this was correlated with a lower level of H2A.Z in the promoter and coding region of the LINE-1 element. Furthermore, when TSCs were differentiated into TGCs in vitro, there was a dramatic reduction in LINE-1 expression and this repression was positively correlated with a gain of H2A.Z on LINE-1s. These observations suggested that H2A.Z was a repressor…

Subjects/Keywords: LINE-1s; H2A.Z; RNAPII; Trophoblast stem cells; CD69

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APA (6^th Edition):

Shen, Q. (2016). Uncovering new functions for histone variants: a role for H2A.Z in silencing retrotransposons . (Thesis). Australian National University. Retrieved from http://hdl.handle.net/1885/104595

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Shen, Qian. “Uncovering new functions for histone variants: a role for H2A.Z in silencing retrotransposons .” 2016. Thesis, Australian National University. Accessed March 09, 2021. http://hdl.handle.net/1885/104595.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Shen, Qian. “Uncovering new functions for histone variants: a role for H2A.Z in silencing retrotransposons .” 2016. Web. 09 Mar 2021.

Vancouver:

Shen Q. Uncovering new functions for histone variants: a role for H2A.Z in silencing retrotransposons . [Internet] [Thesis]. Australian National University; 2016. [cited 2021 Mar 09]. Available from: http://hdl.handle.net/1885/104595.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Shen Q. Uncovering new functions for histone variants: a role for H2A.Z in silencing retrotransposons . [Thesis]. Australian National University; 2016. Available from: http://hdl.handle.net/1885/104595

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Utah State University

9. Suasnavas, Edison A. Characterization and Potential Utility of Porcine Trophoblast-Derived Stem-Like Cells.

Degree: MS, Animal, Dairy, and Veterinary Sciences, 2013, Utah State University

URL: https://digitalcommons.usu.edu/etd/1540

► In mammals, the trophoblast lineage of the embryo is specified before implantation. It is restricted to become the fetal portion of the placenta. We… (more)

▼ In mammals, the trophoblast lineage of the embryo is specified before implantation. It is restricted to become the fetal portion of the placenta. We have isolated and cultured trophoblast-derived cells from day 10 and day 13 porcine embryos. These cells demonstrate morphological and biological characteristics that make them unique. We have demonstrated that these cells can grow in vitro in a defined, serum-replacement medium for over a year without showing any signs of senescence. Trophoblast-derived cells placed into serum-containing medium, however, rapidly senesce and fail to proliferate. Gene expression analysis by RT-PCR and Fluidigm analysis of cells in culture from 0-30 days confirmed expression of genes involved in trophoblast function (CDX2, TEAD4, CYP17A1, HSD17B1, FGFR2, PLET, HAND1) as well as some genes known to mediate pluripotency (POU5F1, KLF4, CMYC). These experiments revealed changes in gene expression over time and in response to serum-containing medium. We have demonstrated that these trophoblast-derived cells are easily stably transfected with an exogenous transgene (eGFP) by a variety of methods, and show the ability to survive and to be passaged repeatedly after transfection. Also, immunofluorescence analysis results demonstrated that these cells do not only demonstrate epithelial characteristics by the expression of KRT18, but also they show expression of VIMENTIN which is a protein found in mesenchymal cells. These findings contradict studies done by Ramsoondar in 1993 and Flechon in 1995 which reported the negative expression of VIMENTIN in similar cells. In summary, early embryonic porcine trophoblast-derived cells have demonstrated unique characteristics which have taken us to the conclusion that they could be used as valuable tools for laboratory work. Anticipated applications include the study of trophoblast physiology as well as possible solutions for improving efficiency of transgenesis by somatic cell nuclear transfer and for pluripotency reprogramming of cells. Advisors/Committee Members: S. Clay Isom, Kenneth L. White, Lee F. Rickords, ;.

Subjects/Keywords: Porcine Trophoblast; Stem-Like Cells; Embryo; Vimentin; Animal Sciences; Molecular Biology

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APA (6^th Edition):

Suasnavas, E. A. (2013). Characterization and Potential Utility of Porcine Trophoblast-Derived Stem-Like Cells. (Masters Thesis). Utah State University. Retrieved from https://digitalcommons.usu.edu/etd/1540

Chicago Manual of Style (16^th Edition):

Suasnavas, Edison A. “Characterization and Potential Utility of Porcine Trophoblast-Derived Stem-Like Cells.” 2013. Masters Thesis, Utah State University. Accessed March 09, 2021. https://digitalcommons.usu.edu/etd/1540.

MLA Handbook (7^th Edition):

Suasnavas, Edison A. “Characterization and Potential Utility of Porcine Trophoblast-Derived Stem-Like Cells.” 2013. Web. 09 Mar 2021.

Vancouver:

Suasnavas EA. Characterization and Potential Utility of Porcine Trophoblast-Derived Stem-Like Cells. [Internet] [Masters thesis]. Utah State University; 2013. [cited 2021 Mar 09]. Available from: https://digitalcommons.usu.edu/etd/1540.

Council of Science Editors:

Suasnavas EA. Characterization and Potential Utility of Porcine Trophoblast-Derived Stem-Like Cells. [Masters Thesis]. Utah State University; 2013. Available from: https://digitalcommons.usu.edu/etd/1540

Brock University

10. Dean, Wendy L. DNA polymerase activity in trophoblast giant cells in the mouse .

Degree: Department of Biological Sciences, 1985, Brock University

URL: http://hdl.handle.net/10464/1506

► In the developing mouse embryo, the diploid trophectoderm is known to undergo a diploid to giant cell transformation. These cells arise by a process of… (more)

▼ In the developing mouse embryo, the diploid trophectoderm is known to undergo a diploid to giant cell transformation. These cells arise by a process of endoreduplication, characterized by replication of the entire genome without subsequent mitosis or cell division, leading to polyploidy and the formation of giant nuclei. Studies of 13.5 day rat trophoblast derived from the parietal yolk sac have indicated a relatively low rate of DNA polymerase a activity, the noinnal eukaryotic replicase, in comparison to that of DNA polymerase g. These results have suggested that endoreduplication in trophoblast giant cells may not employ the normal replicase enzyme, DNA polymerase a. In order to determine whether a 'switch' from DNA polymerase to DNA polymerase is a necessary concomitant of the diploid to giant cell transformation, two distinct populations of trophoblast giant cells, the primary giant cell derived from the mural trophectoderm and the secondary giant cell derived from the polar trophoectoderm were used. These two populations of trophoblast giant cells can be obtained from the tissue outgrowths of 3.5da blastocysts and the extraembryonic ectoderm (EX) and ectoplacental cone (EPC) of 7.5 day embryos respectively. Tissue outgrowths were treated with aphidicolin, a specific reversible inhibitor of eukaryotic DNA polymerase a, on various days after explantation. The effect of aphidicolin treatment was assessed both qualitatively, using autoradiography and quantitatively by scintillation counting and Feulgen staining. 3 DNA synthesis was measured in control and treated cultures after a Hthymidine pulse. Scintillation counts of the embryo proper revealed that DNA synthesis was consistently inhibited by greater than 907. in the presence of aphidicolin. Inhibition of DNA synthesis in the EX and EPC varied between 81-957. and 82-987. respectively, indicating that most DNA synthesis was mediated by DNA polymerase a, but that a small but significant amount of residual synthesis was indicated. A qualitative approach was then applied to determine whether the apparent residual DNA synthesis was restricted to a subpopulation of giant cells or whether all giant cells displayed a low level of DNA synthesis. Autoradiographs of the ICM of blastocysts and the embryo proper of 7.5da embryos, which acted as diploid control population, was completely inhibited regardless of duration in explant culture. In contrast, primary trophoblast giant cells derived from blastocysts and secondary giant cells derived from the EX and EPC were observed to possess some heavily labelled cells after aphidicolin treatment. These results suggest that although DNA polymerase a is the primary replicating enzyme responsible for endoreduplication in mouse trophoblast giant cells, some nonactivity is also observed. A DNA polymerase assay employing tissue lysates of outgrown 7.5da embryo, EX and EPC tissues was used to attempt to confirm the presence of higher nonactivity in tissues possessing trophoblast giant cells.…

Subjects/Keywords: DNA polymerases.; Trophoblast.; Mice; Cells.

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Dean, W. L. (1985). DNA polymerase activity in trophoblast giant cells in the mouse . (Thesis). Brock University. Retrieved from http://hdl.handle.net/10464/1506

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Dean, Wendy L. “DNA polymerase activity in trophoblast giant cells in the mouse .” 1985. Thesis, Brock University. Accessed March 09, 2021. http://hdl.handle.net/10464/1506.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Dean, Wendy L. “DNA polymerase activity in trophoblast giant cells in the mouse .” 1985. Web. 09 Mar 2021.

Vancouver:

Dean WL. DNA polymerase activity in trophoblast giant cells in the mouse . [Internet] [Thesis]. Brock University; 1985. [cited 2021 Mar 09]. Available from: http://hdl.handle.net/10464/1506.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Dean WL. DNA polymerase activity in trophoblast giant cells in the mouse . [Thesis]. Brock University; 1985. Available from: http://hdl.handle.net/10464/1506

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

11. Barreto, Rodrigo da Silva Nunes. Ocorrência e mecanismos do microquimerismo fetal em gestações bovinas.

Degree: Mestrado, Anatomia dos Animais Domésticos e Silvestres, 2011, University of São Paulo

URL: http://www.teses.usp.br/teses/disponiveis/10/10132/tde-28052012-155830/ ;

► O sucesso da gestação depende da adequada comunicação materno-fetal, que em algumas espécies têm um contato mais íntimo devido à capacidade migratória de populações de… (more)

▼ O sucesso da gestação depende da adequada comunicação materno-fetal, que em algumas espécies têm um contato mais íntimo devido à capacidade migratória de populações de células trofoblásticas. Nos bovinos esse mecanismo é realizado pelas células trofoblásticas gigantes (CTGs), com invasão limitada até a lâmina basal do epitélio materno. Apesar dessa leve invasão das CTGs, é possível encontrar células fetais circulantes no sangue periférico da vaca gestante, levando ao microquimerismo fetal. Além de toda uma sinalização local e sistêmica e mudanças conformacionais, a migração das CTGs também é dependente da tolerância imunológica do epitélio materno que possui uma baixa expressão de MHC de classe I. Em contrapartida, o trofoblasto expressa MHC de classe Ib para impedir a ativação das células natural killers uterinas (uNK) contra ele mesmo. Neste contexto, o objetivo desse trabalho foi estudar a ocorrência e contribuir para o entendimento dos mecanismos da migração celular na placenta bovina, com marcadores exclusivos do cromossomo Y e de um modelo de clone transgênico expressando a proteína GFP. A hipótese testada foi que o microquimerismo fetal observado mediante a detecção do gene TSPY no sangue periférico da vaca gestante de embrião macho, e de GFP nos tecidos placentários maternos, associado à expressão de MHC classe 1b (Qa2) na interface materno-fetal. Para tanto, 153 embriões produzidos por fertilização in vitro (FIV) foram transferidos, resultando em 34 embriões machos e 31 fêmeas no dia 62 de gestação, quando foi realizada a coleta de sangue periférico da receptora. Dentre estas gestações, foram selecionadas de 25 machos, 4 fêmeas e 5 perdas gestacionais (confirmadas no D39 por ultrassonografia) para detecção de TSPY. Também foram produzidas gestações de clones transgênicos, expressando GFP com 30, 60 e 90 dias que foram utilizadas para a detecção de mRNA e a proteína GFP. Nas gestações de FIV 60% dos embriões machos, 50% das fêmeas e 40% das perdas gestacionais foram positivos para TSPY. A detecção de TSPY nas gestações de fêmeas possivelmente é resultante da persistência do microquimerismo de gestações anteriores. Nas gestações de clones transgênicos, observou-se a presença de mRNA e proteína GFP no endométrio, também indicando migração nesta região ou o transporte da GFP, e outros conteúdos do trofoblasto, para o epitélio materno. Nos placentônios, usando anticorpo anti-GFP pode-se ver a marcação positiva tanto no trofoblasto como no epitélio materno, possivelmente decorrente de liberação das CTGs no estroma endometrial após a fusão. As CTGs, quando em formação sincicial, têm a sua expressão de GFP diminuída, o que também foi observado, utilizando-se anticorpo anti-Qa2 (antígeno murino para MHC classe Ib). O epitélio materno e o trofoblástico também foram marcados para Qa2. Mediante as técnicas utilizadas, observamos que o microquimerismo pôde ser identificado nas gestações analisadas com o uso dos marcadores TSPY no sangue e o GFP nos tecidos placentários maternos. Este estudo mostra que na… Advisors/Committee Members: Meirelles, Flávio Vieira.

Subjects/Keywords: bovine placentation; células trofoblásticas gigantes; fetal microchimerism; invasão trofoblástica; Microquimerismo fetal; placentação bovina; trophoblast giant cells; trophoblast invasion; TSPY; TSPY

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Barreto, R. d. S. N. (2011). Ocorrência e mecanismos do microquimerismo fetal em gestações bovinas. (Masters Thesis). University of São Paulo. Retrieved from http://www.teses.usp.br/teses/disponiveis/10/10132/tde-28052012-155830/ ;

Chicago Manual of Style (16^th Edition):

Barreto, Rodrigo da Silva Nunes. “Ocorrência e mecanismos do microquimerismo fetal em gestações bovinas.” 2011. Masters Thesis, University of São Paulo. Accessed March 09, 2021. http://www.teses.usp.br/teses/disponiveis/10/10132/tde-28052012-155830/ ;.

MLA Handbook (7^th Edition):

Barreto, Rodrigo da Silva Nunes. “Ocorrência e mecanismos do microquimerismo fetal em gestações bovinas.” 2011. Web. 09 Mar 2021.

Vancouver:

Barreto RdSN. Ocorrência e mecanismos do microquimerismo fetal em gestações bovinas. [Internet] [Masters thesis]. University of São Paulo; 2011. [cited 2021 Mar 09]. Available from: http://www.teses.usp.br/teses/disponiveis/10/10132/tde-28052012-155830/ ;.

Council of Science Editors:

Barreto RdSN. Ocorrência e mecanismos do microquimerismo fetal em gestações bovinas. [Masters Thesis]. University of São Paulo; 2011. Available from: http://www.teses.usp.br/teses/disponiveis/10/10132/tde-28052012-155830/ ;

12. Borbely, Alexandre Urban. A influência do biglicam mediada por receptores do tipo Toll-like 2 e 4 no processo de invasão das células trofoblásticas.

Degree: PhD, Biologia Celular e Tecidual, 2013, University of São Paulo

URL: http://www.teses.usp.br/teses/disponiveis/42/42134/tde-29052014-132134/ ;

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O biglicam é um proteoglicano é altamente expresso em células trofoblásticas de patologias placentárias com invasividade exacerbada. No entanto, as funções do biglicam no trofoblasto… (more)

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O biglicam é um proteoglicano é altamente expresso em células trofoblásticas de patologias placentárias com invasividade exacerbada. No entanto, as funções do biglicam no trofoblasto ainda não foram elucidadas. Sendo assim, verificamos a expressão e as funções de biglicam e seus receptores Toll-like (TLR)-2 e TLR-4 nas células trofoblásticas durante a gestação. As células do citotrofoblasto extraviloso (CTEV) foram positivas para todas as moléculas, menos para o biglicam em placentas a termo. Adição exógena de biglicam promoveu migração e invasão das células trofoblásticas. O biglicam estimulou a fosforilação de AKT nos sítios Thr308 e Ser473 nas células trofoblásticas. A migração e a invasão biglicam-dependentes e as fosforilações de AKT foram inibidas após a adição de anticorpos bloqueadores anti-TLR-2 e anti-TLR-4. O silenciamento gênico de AKT1 em células SGHPL-5 aboliu os efeitos do biglicam na motilidade. Em conclusão, o biglicam aumenta a motilidade de células trofoblásticas após sinalização por AKT através da ativação de TLR-2 e TLR-4.

Biglycan is a highly expressed proteoglycan in trophoblast cells from invasiveness-changed placental pathologies. However, biglycan functions in the trophoblast were not yet identified. Therefore, it was verified the expression and functions of biglycan and its receptors Toll-like (TLR)-2 and TLR-4 in trophoblast cells throughout pregnancy. The extravillous cytotrophoblast cells (EVT) were positive to all the molecules, although biglycan was negative in term placentas. Exogenous biglycan promoted migration and invasion of trophoblast cells. Biglycan stimulated AKT phosphorilation at Thr308 and Ser473 sites in trophoblast cells. The biglycan-dependent migration, invasion and AKT phosphorilation were inhibited upon addiction of anti-TLR-2 and anti-TLR-4 blocking antibodies. AKT1 genic silencing in SGHPL-5 cells abolished the motility effects. In conclusion, biglycan increases the motility of trophoblast cells after AKT signaliing throughout TLR-2 and TLR-4 activation.

Advisors/Committee Members: Bevilacqua, Estela Maris Andrade Forell.

Subjects/Keywords: Cellular receptors; Células trofoblásticas; Chorionic gonadotropin; Gonadotrofina coriônica; Proteoglicanas; Proteoglycans; Receptores celulares; Trofoblasto; Trophoblast; Trophoblast cells

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Borbely, A. U. (2013). A influência do biglicam mediada por receptores do tipo Toll-like 2 e 4 no processo de invasão das células trofoblásticas. (Doctoral Dissertation). University of São Paulo. Retrieved from http://www.teses.usp.br/teses/disponiveis/42/42134/tde-29052014-132134/ ;

Chicago Manual of Style (16^th Edition):

Borbely, Alexandre Urban. “A influência do biglicam mediada por receptores do tipo Toll-like 2 e 4 no processo de invasão das células trofoblásticas.” 2013. Doctoral Dissertation, University of São Paulo. Accessed March 09, 2021. http://www.teses.usp.br/teses/disponiveis/42/42134/tde-29052014-132134/ ;.

MLA Handbook (7^th Edition):

Borbely, Alexandre Urban. “A influência do biglicam mediada por receptores do tipo Toll-like 2 e 4 no processo de invasão das células trofoblásticas.” 2013. Web. 09 Mar 2021.

Vancouver:

Borbely AU. A influência do biglicam mediada por receptores do tipo Toll-like 2 e 4 no processo de invasão das células trofoblásticas. [Internet] [Doctoral dissertation]. University of São Paulo; 2013. [cited 2021 Mar 09]. Available from: http://www.teses.usp.br/teses/disponiveis/42/42134/tde-29052014-132134/ ;.

Council of Science Editors:

Borbely AU. A influência do biglicam mediada por receptores do tipo Toll-like 2 e 4 no processo de invasão das células trofoblásticas. [Doctoral Dissertation]. University of São Paulo; 2013. Available from: http://www.teses.usp.br/teses/disponiveis/42/42134/tde-29052014-132134/ ;

University of Waikato

13. Deane, Jessica Robyn. Targets of Elf5 in Mouse Trophoblast Stem Cells .

Degree: 2007, University of Waikato

URL: http://hdl.handle.net/10289/7506

► The Placenta is an essential organ for all mammalian embryonic development as it provides the nutritional link between maternal and foetal blood streams. The cells… (more)

▼ The Placenta is an essential organ for all mammalian embryonic development as it provides the nutritional link between maternal and foetal blood streams. The cells which go on to proliferate and contribute to all the major cell types of the embryo derived placenta have been located to the trophoblast (TE) cells overlying the Inner Cell Mass (ICM) of the embryo. Immortal cell lines have been subsequentially derived from this tissue and called Trophoblast Stem (TS) cells. In parallel with their in vivo counterparts they are also reliant on Fibroblast growth factor 4 (FgF4) (Tanaka et al., 1998) and Activin/Nodal signalling (Guzman-Ayala et al., 2004). The Ets family transcription factor, Elf5, has been shown to be specifically expressed in the early placental trophoblast and subsequent derived tissues. Mice deficient in Elf5 failed to form a placenta post implantation. Furthermore TS cells were unable to be derived from Elf5 knockout embryos (Donnison et al., 2005). This work suggested that Elf5 plays an essential role in TS cells and their differentiation. The aim of this study was to determine the downstream target genes of Elf5 in mouse TS cells. The target genes of Fgf4 and Activin/Nodal signalling in TS cells were also investigated. This work is hoped to contribute to an overall greater understanding of the molecular networks underlying TS cell maintenance and to contribute to our knowledge of early placental development. Small interfering RNA (siRNA) targeted reduction of Elf5 mRNA expression in mTS cells was achieved using two independent siRNAs; with Elf5 reduction exceeding 80%. The resulting changes in gene expression were measured in order to determine the downstream targets of Elf5. Selected genes known to be important for trophoblast differentiation and maintenance were measured using real-time PCR in a candidate gene approach .Global changes in gene expression as a consequence of Elf5 silencing were measured using an Affymetrix microarray. Global changes in gene expression due to growth factor (Fgf4 and/or Activin) removal were also measured. Expression of 22 genes was changed using either of the Elf5 siRNA oligonucleotides. Of these, 9 were also significantly changed by growth factor removal. Included in this set were Synopl, Hst3st3b1, Cyr61 and Sox2. In the overall analysis, many genes whose expression changed upon loss of Elf5 are known to play important roles in trophectoderm cell specification. Real-time PCR validation agreed closely with the up or down regulation measured using the microarray. This work has thus led to the discovery of sets of Elf5 target genes potentially involved in trophoblast stem cell function and has provided the foundation for future work exploring the molecular pathways of trophoblast development. Advisors/Committee Members: McLeay, Lance M (advisor).

Subjects/Keywords: Elf5; trophoblast; placenta; development; trophoblast stem cells

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Deane, J. R. (2007). Targets of Elf5 in Mouse Trophoblast Stem Cells . (Masters Thesis). University of Waikato. Retrieved from http://hdl.handle.net/10289/7506

Chicago Manual of Style (16^th Edition):

Deane, Jessica Robyn. “Targets of Elf5 in Mouse Trophoblast Stem Cells .” 2007. Masters Thesis, University of Waikato. Accessed March 09, 2021. http://hdl.handle.net/10289/7506.

MLA Handbook (7^th Edition):

Deane, Jessica Robyn. “Targets of Elf5 in Mouse Trophoblast Stem Cells .” 2007. Web. 09 Mar 2021.

Vancouver:

Deane JR. Targets of Elf5 in Mouse Trophoblast Stem Cells . [Internet] [Masters thesis]. University of Waikato; 2007. [cited 2021 Mar 09]. Available from: http://hdl.handle.net/10289/7506.

Council of Science Editors:

Deane JR. Targets of Elf5 in Mouse Trophoblast Stem Cells . [Masters Thesis]. University of Waikato; 2007. Available from: http://hdl.handle.net/10289/7506

University of Toronto

14. Park, Brian Jungmin. Subcellular Dynamics of RNAs and microRNAs in Mouse Embryonic and Trophoblast Stem Cells.

Degree: 2020, University of Toronto

URL: http://hdl.handle.net/1807/103518

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Cell fractionation coupled to high-throughput RNA-sequencing allows for the identification of cytoplasmic and nuclear over-represented RNA populations. In mouse embryonic and trophoblast stem cells, an… (more)

Subjects/Keywords: embryonic stem cells; intron retention; microRNA; RNA-seq; stem cell biology; trophoblast stem cells; 0715

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Park, B. J. (2020). Subcellular Dynamics of RNAs and microRNAs in Mouse Embryonic and Trophoblast Stem Cells. (Masters Thesis). University of Toronto. Retrieved from http://hdl.handle.net/1807/103518

Chicago Manual of Style (16^th Edition):

Park, Brian Jungmin. “Subcellular Dynamics of RNAs and microRNAs in Mouse Embryonic and Trophoblast Stem Cells.” 2020. Masters Thesis, University of Toronto. Accessed March 09, 2021. http://hdl.handle.net/1807/103518.

MLA Handbook (7^th Edition):

Park, Brian Jungmin. “Subcellular Dynamics of RNAs and microRNAs in Mouse Embryonic and Trophoblast Stem Cells.” 2020. Web. 09 Mar 2021.

Vancouver:

Park BJ. Subcellular Dynamics of RNAs and microRNAs in Mouse Embryonic and Trophoblast Stem Cells. [Internet] [Masters thesis]. University of Toronto; 2020. [cited 2021 Mar 09]. Available from: http://hdl.handle.net/1807/103518.

Council of Science Editors:

Park BJ. Subcellular Dynamics of RNAs and microRNAs in Mouse Embryonic and Trophoblast Stem Cells. [Masters Thesis]. University of Toronto; 2020. Available from: http://hdl.handle.net/1807/103518

University of Waikato

15. Redgate, Emma Louise. The Role of ELf5 in Mouse Trophoblast Stem Cells .

Degree: 2010, University of Waikato

URL: http://hdl.handle.net/10289/4392

► Elf5 is a DNA transcription factor that has been identified as being involved in placentation of the early embryo. Elf5 is expressed in the extra… (more)

▼ Elf5 is a DNA transcription factor that has been identified as being involved in placentation of the early embryo. Elf5 is expressed in the extra embryonic ectoderm (ExE), a lineage that contributes to the placenta of the embryo. Pluripotent trophoblast stem (TS) cells can be derived from the ExE and can be cultured in vitro. Such cells can contribute to all the placental lineages when injected back into an embryo. Elf5 homozygous mutant embryos do not possess an ExE and trophoblast stem cells cannot be derived and the mutation is therefore embryonic lethal. When Elf5 is knocked out, the TS cells in the ExE are thought to differentiate into EPC/giant cells leading to the absence of the ExE. In previous experiments in this laboratory, potential Elf5 targets were identified by RNA interference in mouse trophoblast stem cells followed by global gene expression analysis using an Affymetrix array. In the present experiments these results have now been confirmed by siRNA induced knockdown of Elf5 in TS cells followed by quantitative real time PCR of potential target genes. Most of the target genes that were affected by Elf5 knockdown were changed in the same way by growth factor removal and therefore stem cell differentiation. This suggested that Elf5 usually acts to maintain the TS cells in a stem cell fate. As Elf5 is expressed in the ExE, whole mount In situ hybridisation was used to determine if the genes showed the correct spatial and temporal patterning to be in vivo relevant Elf5 targets. The targets that were down regulated upon Elf5 knockdown (and therefore positively regulated by Elf5) were expressed in the ExE as expected. Genes that were up-regulated upon Elf5 knockdown (and therefore usually repressed by Elf5) were expressed in the differentiated ectoplacental cone or giant cells. . Preliminary work was also carried out for the over-expression of Elf5 by a tamoxifen inducible Elf5. TS cells were stably transfected with a plasmid containing Elf5 fused to a tamoxifen inducible ERT2 receptor and a VP16 transcriptional activation domain to turn Elf5 into a potent activator, and were analysed for target gene expression. Preliminary data showed that Elf5 is involved in a complex transcriptional network of events as the genes did not behave as expected when Elf5 was turned into an activator. The effect of Elf5 knockdown on TS cells has been studied closely in terms of morphology, proliferation, changes in DNA content and apoptosis. The knockdown of Elf5 caused an increase in the number of differentiated cells (as shown by changes in morphology and DNA content). This further supported the knockdown and in situ data. There was no change in proliferation or apoptosis. These experiments which demonstrate which genes are regulated by Elf5 and the changes to TS cellular characteristics when Elf5 is knocked down, support the proposal that Elf5 acts as a trophoblast stem cell maintenance factor of the extraembryonic ectoderm of the mouse. Advisors/Committee Members: McLeay, Lance M (advisor), Pfeffer, Peter (advisor).

Subjects/Keywords: Trophoblast; Trophoblast stem cells; Elf5; placenta; ExE; giant cells; siRNA

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Redgate, E. L. (2010). The Role of ELf5 in Mouse Trophoblast Stem Cells . (Masters Thesis). University of Waikato. Retrieved from http://hdl.handle.net/10289/4392

Chicago Manual of Style (16^th Edition):

Redgate, Emma Louise. “The Role of ELf5 in Mouse Trophoblast Stem Cells .” 2010. Masters Thesis, University of Waikato. Accessed March 09, 2021. http://hdl.handle.net/10289/4392.

MLA Handbook (7^th Edition):

Redgate, Emma Louise. “The Role of ELf5 in Mouse Trophoblast Stem Cells .” 2010. Web. 09 Mar 2021.

Vancouver:

Redgate EL. The Role of ELf5 in Mouse Trophoblast Stem Cells . [Internet] [Masters thesis]. University of Waikato; 2010. [cited 2021 Mar 09]. Available from: http://hdl.handle.net/10289/4392.

Council of Science Editors:

Redgate EL. The Role of ELf5 in Mouse Trophoblast Stem Cells . [Masters Thesis]. University of Waikato; 2010. Available from: http://hdl.handle.net/10289/4392

Univerzitet u Beogradu

16. Ćujić, Danica R., 1979-. Uticaj steroidnih hormona i njihovih antagonista na nivoe galektina u ćelijama trofoblasta čoveka in vitro.

Degree: Farmaceutski fakultet, 2016, Univerzitet u Beogradu

URL: https://fedorabg.bg.ac.rs/fedora/get/o:10300/bdef:Content/get

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Farmacija - Medicinska biohemija / Pharmacy - Medical Biochemistry

Galektini su evolutivno očuvana grupa lektina, sa afinitetom prema β-galaktozidnoj strukturi. Zahvaljujući lektinskoj aktivnosti, galektini su… (more)

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Farmacija - Medicinska biohemija / Pharmacy - Medical Biochemistry

Galektini su evolutivno očuvana grupa lektina, sa afinitetom prema β-galaktozidnoj strukturi. Zahvaljujući lektinskoj aktivnosti, galektini su uključeni u regulaciju vitalnih ćelijskih procesa kao što su preživljavanje ćelija, apoptoza, regulacija ćelijskog ciklusa, obrada iRNK transkripta, ćelijska adhezija i migracija. Postoje indicije da su galektini uključeni u različite reproduktivne procese, uključujući i proces implantacije embriona i placentacije, kao preduslova za razvoj fetusa. Iako mehanizmi nisu nedovoljno poznati, ekspresija galektina je precizno regulisana i može biti specifična za određeno tkivo ili fazu razvića. Steroidni hormoni imaju važnu ulogu u pripremi, uspostavljanju i održavanju trudnoće. Ćelije ekstravilusnog trofoblalsta (EVT) ne sintetišu steroidne hormone, ali prisustvo odgovarajućih receptora omogućava da steroidni hormoni mogu ispoljiti svoje delovanje na ove ćelije. U ćelijama EVT je pokazano prisustvo galektina-1, -3 i -8. Poznavanje regulacije ekspresije galektina u EVT je veoma oskudno, ali postoje podaci za druga tkiva koji ukazuju na ulogu steroidnih hormona. U promotorskim regionima gena za ova tri galektina prisutne su odgovarajuće hormon-response sekvence, pa postoji mogućnost da steroidni hormoni mogu uticati na ekspresiju galektina-1, -3 i -8. U ovom radu je ispitan uticaj steroidnih hormona progesterona (P4), 17β-estradiola (E2) i testosterona (TE), zatim sintetskog analoga kortizola, deksametazona (DEX), kao i odgovarajućih antagonista steroidnih hormona na nivoe galektina-1, -3 i -8 u EVT ćelijskoj liniji HTR-8/SVneo. Efekat je praćen na nivou genske ekspresije, sadržaja galektina unutar ćelija, kao i mogućim promenama u sekreciji. Dobijeni rezultati su pokazali da su galektini-1, -3 i -8 u EVT ćelijskoj liniji HTR-8/SVneo modulirani steroidnim hormonima, steroid specifično i zavisno od doze. Steroidni hormoni mogu različito delovati na transkripciju gena (iRNK), na nivoe intracelularnih galektina, odnosno na galektine sekretovane van ćelija. Nivo iRNK za LGALS1, kao i nivoi galektina-1 u ćelijama i galektin-1 oslobađen van ćelija su bili povišeni pri koncentraciji od 10 nM E2, dok su pri višoj koncentraciji (1000 nM) bili smanjeni. S druge strane, nivo proteina galektina-3 i njegova sekrecija su stimulisani P4 (10 nM). U prisustvu mifepristona, antagonista glukokoritikoida i progesterona, uočen je efekat na nivoe galektina-1 i -3 suprotan u odnosu na P4, što sugeriše da je ova regulacija posredovana progesteronskim receptorom. Iako su uočene najmanje promene kod galektina-8 pod uticajem steroidnih hormona, njegova sekrecija je povećana nakon tretmana P4, DEX i E2 (10 nM)...

Advisors/Committee Members: Spasić, Slavica, 1947-.

Subjects/Keywords: galectin; trophoblast; HTR-8/SVneo cells; steroid hormone; regulation; expression; secretion; placental galectin-1; ELISA

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Ćujić, Danica R., 1. (2016). Uticaj steroidnih hormona i njihovih antagonista na nivoe galektina u ćelijama trofoblasta čoveka in vitro. (Thesis). Univerzitet u Beogradu. Retrieved from https://fedorabg.bg.ac.rs/fedora/get/o:10300/bdef:Content/get

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Ćujić, Danica R., 1979-. “Uticaj steroidnih hormona i njihovih antagonista na nivoe galektina u ćelijama trofoblasta čoveka in vitro.” 2016. Thesis, Univerzitet u Beogradu. Accessed March 09, 2021. https://fedorabg.bg.ac.rs/fedora/get/o:10300/bdef:Content/get.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Ćujić, Danica R., 1979-. “Uticaj steroidnih hormona i njihovih antagonista na nivoe galektina u ćelijama trofoblasta čoveka in vitro.” 2016. Web. 09 Mar 2021.

Vancouver:

Ćujić, Danica R. 1. Uticaj steroidnih hormona i njihovih antagonista na nivoe galektina u ćelijama trofoblasta čoveka in vitro. [Internet] [Thesis]. Univerzitet u Beogradu; 2016. [cited 2021 Mar 09]. Available from: https://fedorabg.bg.ac.rs/fedora/get/o:10300/bdef:Content/get.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Ćujić, Danica R. 1. Uticaj steroidnih hormona i njihovih antagonista na nivoe galektina u ćelijama trofoblasta čoveka in vitro. [Thesis]. Univerzitet u Beogradu; 2016. Available from: https://fedorabg.bg.ac.rs/fedora/get/o:10300/bdef:Content/get

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Western Ontario

17. Awamleh, Zain. Placental MicroRNA Expression in Pregnancies Complicated with Preeclampsia and Intrauterine Growth Restriction.

Degree: 2019, University of Western Ontario

URL: https://ir.lib.uwo.ca/etd/6107

► A normally developed placenta is integral to a successful pregnancy. Preeclampsia (PE) and intrauterine growth restriction (IUGR) are two common pregnancy related complications that result… (more)

▼ A normally developed placenta is integral to a successful pregnancy. Preeclampsia (PE) and intrauterine growth restriction (IUGR) are two common pregnancy related complications that result from abnormal placental development. Placental microRNAs (miRNAs) have been investigated as potential biomarkers for these complications, but they may also play a role in placental development and pathophysiology by influencing gene expression. The objectives of this study are (i) to utilize next-generation sequencing to determine miRNA and gene expression in human placental (chorionic villous) samples from three distinct patient groups with early-onset (EO) PE, IUGR, or PE + IUGR, and (ii) integration of expression datasets to assess the impact of dysregulated miRNAs on gene expression and trophoblast cell function.Placental tissues were collected from four patient groups (control [N=21], EO-PE [N=20], EO-IUGR [N=18], and EO-PE + IUGR [N=20]), and totalRNA was used for miRNA and RNA sequencing. Multiple differential expression analysis programs were used to analyze both expression datasets in each patient group compared to gestational age-matched controls. Inverse correlation analysis and target prediction software were used to identify gene targets. Candidate gene targets identified were confirmed using luciferase assays, and impact of miRNAs on trophoblast function was assessed using proliferation and migration assays in HTR-8/SVneo cells.Analysis revealed miRNAs and genes that are disease-specific, as well as others that are common between disease groups, 6 microRNAs and 22 genes were identified to be differentially expressed in all three patient groups. In addition, integrative analysis between the miRNA and gene expression datasets revealed candidate gene targets for miR-193b-5p and miR-210-5p, two miRNAs that also have an impact on trophoblast cell functions. Our findings suggest common underlying placental pathologies in EO-PE and EO-IUGR.Dysregulated miRNAs and genes identified in this study provide further evidence for trophoblast dysfunction in these pregnancy complications. Integration of miRNA and RNA profiling in the same three subgroups of pregnancy complications, provides an alternate level of molecular information and is a useful tool to expand our understanding of molecular perturbations in the placenta in early onset diseases.

Subjects/Keywords: Gene Expression; Intrauterine Growth Restriction; MicroRNA Expression; Placenta; Preeclampsia; Trophoblast Cells; Molecular Biology

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Awamleh, Z. (2019). Placental MicroRNA Expression in Pregnancies Complicated with Preeclampsia and Intrauterine Growth Restriction. (Thesis). University of Western Ontario. Retrieved from https://ir.lib.uwo.ca/etd/6107

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Awamleh, Zain. “Placental MicroRNA Expression in Pregnancies Complicated with Preeclampsia and Intrauterine Growth Restriction.” 2019. Thesis, University of Western Ontario. Accessed March 09, 2021. https://ir.lib.uwo.ca/etd/6107.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Awamleh, Zain. “Placental MicroRNA Expression in Pregnancies Complicated with Preeclampsia and Intrauterine Growth Restriction.” 2019. Web. 09 Mar 2021.

Vancouver:

Awamleh Z. Placental MicroRNA Expression in Pregnancies Complicated with Preeclampsia and Intrauterine Growth Restriction. [Internet] [Thesis]. University of Western Ontario; 2019. [cited 2021 Mar 09]. Available from: https://ir.lib.uwo.ca/etd/6107.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Awamleh Z. Placental MicroRNA Expression in Pregnancies Complicated with Preeclampsia and Intrauterine Growth Restriction. [Thesis]. University of Western Ontario; 2019. Available from: https://ir.lib.uwo.ca/etd/6107

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Utah State University

18. Leppo, Kelsy A. Identification of Bovine T-Cell Populations Involved in Placental Growth and Development.

Degree: MS, Animal, Dairy, and Veterinary Sciences, 2020, Utah State University

URL: https://digitalcommons.usu.edu/etd/7939

► Today, the dairy industry is comprised of cows that can produce around 20,000 pounds of milk in a year, yet producers often see only… (more)

Subjects/Keywords: lymphocytes; immune cells; bovine; pregnancy; placenta; trophoblast; maternal-fetal interface; Veterinary Medicine

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Leppo, K. A. (2020). Identification of Bovine T-Cell Populations Involved in Placental Growth and Development. (Masters Thesis). Utah State University. Retrieved from https://digitalcommons.usu.edu/etd/7939

Chicago Manual of Style (16^th Edition):

Leppo, Kelsy A. “Identification of Bovine T-Cell Populations Involved in Placental Growth and Development.” 2020. Masters Thesis, Utah State University. Accessed March 09, 2021. https://digitalcommons.usu.edu/etd/7939.

MLA Handbook (7^th Edition):

Leppo, Kelsy A. “Identification of Bovine T-Cell Populations Involved in Placental Growth and Development.” 2020. Web. 09 Mar 2021.

Vancouver:

Leppo KA. Identification of Bovine T-Cell Populations Involved in Placental Growth and Development. [Internet] [Masters thesis]. Utah State University; 2020. [cited 2021 Mar 09]. Available from: https://digitalcommons.usu.edu/etd/7939.

Council of Science Editors:

Leppo KA. Identification of Bovine T-Cell Populations Involved in Placental Growth and Development. [Masters Thesis]. Utah State University; 2020. Available from: https://digitalcommons.usu.edu/etd/7939

University of Toronto

19. Miri, Kamelia. The Imprinted Polycomb Group Gene Sfmbt2 is Required for Trophoblast Maintenance and Placenta Development.

Degree: PhD, 2014, University of Toronto

URL: http://hdl.handle.net/1807/94559

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Embryos with two maternal copies of the genome and no paternal contribution (parthenogenetic embryos), die early in development due to failure to sustain a functional… (more)

Subjects/Keywords: Extraembryonic Tissue; Imprinting; Placenta; Polycomb Group Protein; SFMBT2; Trophoblast Stem Cells; 0307

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Miri, K. (2014). The Imprinted Polycomb Group Gene Sfmbt2 is Required for Trophoblast Maintenance and Placenta Development. (Doctoral Dissertation). University of Toronto. Retrieved from http://hdl.handle.net/1807/94559

Chicago Manual of Style (16^th Edition):

Miri, Kamelia. “The Imprinted Polycomb Group Gene Sfmbt2 is Required for Trophoblast Maintenance and Placenta Development.” 2014. Doctoral Dissertation, University of Toronto. Accessed March 09, 2021. http://hdl.handle.net/1807/94559.

MLA Handbook (7^th Edition):

Miri, Kamelia. “The Imprinted Polycomb Group Gene Sfmbt2 is Required for Trophoblast Maintenance and Placenta Development.” 2014. Web. 09 Mar 2021.

Vancouver:

Miri K. The Imprinted Polycomb Group Gene Sfmbt2 is Required for Trophoblast Maintenance and Placenta Development. [Internet] [Doctoral dissertation]. University of Toronto; 2014. [cited 2021 Mar 09]. Available from: http://hdl.handle.net/1807/94559.

Council of Science Editors:

Miri K. The Imprinted Polycomb Group Gene Sfmbt2 is Required for Trophoblast Maintenance and Placenta Development. [Doctoral Dissertation]. University of Toronto; 2014. Available from: http://hdl.handle.net/1807/94559

University of Missouri – Columbia

20. Matsuyama, Haruyo. Generation and characteriazation of porcine induced trophoblast: Generation and characterization of porcine induced trophoblast.

Degree: 2013, University of Missouri – Columbia

URL: https://doi.org/10.32469/10355/40224

► [ACCESS RESTRICTED TO THE UNIVERSITY OF MISSOURI AT AUTHOR'S REQUEST.] Trophoblast stem cells are progenitors of all trophoblast lineages that compose fetal placental portion. Trophoblast… (more)

Subjects/Keywords: Trophoblast – Animal models; Stem cells – Animal models; Cell proliferation – Molecular aspects – Animal models

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APA (6^th Edition):

Matsuyama, H. (2013). Generation and characteriazation of porcine induced trophoblast: Generation and characterization of porcine induced trophoblast. (Thesis). University of Missouri – Columbia. Retrieved from https://doi.org/10.32469/10355/40224

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Matsuyama, Haruyo. “Generation and characteriazation of porcine induced trophoblast: Generation and characterization of porcine induced trophoblast.” 2013. Thesis, University of Missouri – Columbia. Accessed March 09, 2021. https://doi.org/10.32469/10355/40224.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Matsuyama, Haruyo. “Generation and characteriazation of porcine induced trophoblast: Generation and characterization of porcine induced trophoblast.” 2013. Web. 09 Mar 2021.

Vancouver:

Matsuyama H. Generation and characteriazation of porcine induced trophoblast: Generation and characterization of porcine induced trophoblast. [Internet] [Thesis]. University of Missouri – Columbia; 2013. [cited 2021 Mar 09]. Available from: https://doi.org/10.32469/10355/40224.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Matsuyama H. Generation and characteriazation of porcine induced trophoblast: Generation and characterization of porcine induced trophoblast. [Thesis]. University of Missouri – Columbia; 2013. Available from: https://doi.org/10.32469/10355/40224

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

21. Birck, Arlei José. Expressão das conexinas 32 e 43 em células trofoblásticas da placenta bovina em cultura celular.

Degree: PhD, Anatomia dos Animais Domésticos e Silvestres, 2007, University of São Paulo

URL: http://www.teses.usp.br/teses/disponiveis/10/10132/tde-13022008-165545/ ;

►

A expressão da conexina 32 e 43 nas células gigantes trofoblásticas foi analisada em condições de cultura celular com e sem influência de hormônios sexuais.… (more)

▼

A expressão da conexina 32 e 43 nas células gigantes trofoblásticas foi analisada em condições de cultura celular com e sem influência de hormônios sexuais. Placentônios bovinos foram coletados de vacas prenhes em abatedouro nos diferentes períodos gestacionais e divididos em três grupos, primeiro terço (I), segundo terço (II), terceiro terço (III) e transportados ao laboratório em condições assépticas à temperatura de 4ºC em solução de PBS com antibiótico. No laboratório as células foram isoladas e cultivadas em meio D-MEM com 10% SFB por cinco dias. As detecções das conexinas 32 e 43 foram realizadas através de munofluorescencia, pelo método de amplificação da tiramida-fluoresceina, utilizando anticorpo primário policlonal a imunoglobulina de coelho, anti-conexina 32 e 43 de camundongo. Os resultados mostraram que as células gigantes trofoblásticas expressam conexinas 32 e 43 nos três períodos gestacionais com exceção da Cx32 no primeiro terço gestacional sem adição de hormônios, a qual passou a expressar fluorescência após a adição de hormônios. A distribuição da Cx43 evoluiu com a progressão da gestação, permanecendo limitada ao interior das células gigantes trofoblásticas, sem formar junções comunicantes. O terceiro terço gestacional mostra a Cx43 na CGTT localizada no interior do núcleo. A adição de hormônios ao meio de cultura vem confirmar que a progesterona e o estrógeno podem ter um papel no controle da Cx32. Para Cx43 onde se adicionou progesterona os níveis de expressão foram baixos no início aumentando no decorrer da gestação, o mesmo foi encontrado para o conjugado de progesterona/ estrógeno. Para o estrógeno no grupo I os níveis de expressão foram menores se comparados aos três grupos diminuindo no grupo II e voltando a aumentar no grupo III.

The trophoblast cells expression of connexins 32 and 43 was studied in cell culture conditions with or without influence of sexual hormones. Bovine placentomes were collected from pregnant cows in slaughterhouses in different gestational periods and so divided into three groups, first term (I), second term (II), and third term (III), and transported to the laboratory in aseptic conditions at a temperature of 40C in PBS solution with antibiotics. At the laboratory the cells were isolated and cultivated in D-MEM environment with 10% SFB for five days. The detections of connexins 32 and 43 were achieved through immunofluorescence, by the tyramide-fluorescein amplification method, using a rabbit polyclonal primary antibody anti-connexin 32 and 43 of mice. The results showed that the trophoblast cells expressed connexins 32 and 43 in the three gestational periods with an exception of the Cx32 at the first gestational period. However, after the addition of sexual hormones, they began to express the connexins in the cytoplasm in the first stage. The distribution of the Cx43 evolved with the progression of the gestation, remaining limited to the trophoblast`s cells interior, without formation of gap junctions. The third gestational term shows the Cx43 located inside…

Advisors/Committee Members: Hernandez-Blazquez, Francisco Javier.

Subjects/Keywords: Bovinos; Cell culture; Células gigantes trofoblásticas; Conexina; Connexin; Cow; Cultivo celular; Placenta; Placenta; Trophoblast giant cells

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Birck, A. J. (2007). Expressão das conexinas 32 e 43 em células trofoblásticas da placenta bovina em cultura celular. (Doctoral Dissertation). University of São Paulo. Retrieved from http://www.teses.usp.br/teses/disponiveis/10/10132/tde-13022008-165545/ ;

Chicago Manual of Style (16^th Edition):

Birck, Arlei José. “Expressão das conexinas 32 e 43 em células trofoblásticas da placenta bovina em cultura celular.” 2007. Doctoral Dissertation, University of São Paulo. Accessed March 09, 2021. http://www.teses.usp.br/teses/disponiveis/10/10132/tde-13022008-165545/ ;.

MLA Handbook (7^th Edition):

Birck, Arlei José. “Expressão das conexinas 32 e 43 em células trofoblásticas da placenta bovina em cultura celular.” 2007. Web. 09 Mar 2021.

Vancouver:

Birck AJ. Expressão das conexinas 32 e 43 em células trofoblásticas da placenta bovina em cultura celular. [Internet] [Doctoral dissertation]. University of São Paulo; 2007. [cited 2021 Mar 09]. Available from: http://www.teses.usp.br/teses/disponiveis/10/10132/tde-13022008-165545/ ;.

Council of Science Editors:

Birck AJ. Expressão das conexinas 32 e 43 em células trofoblásticas da placenta bovina em cultura celular. [Doctoral Dissertation]. University of São Paulo; 2007. Available from: http://www.teses.usp.br/teses/disponiveis/10/10132/tde-13022008-165545/ ;

22. Kawaguchi, Takamasa. Studies on induction of pluripotency in bovine somatic cells and generation of induced pluripotent stem cells : ウシ体細胞の多能性誘導と人工多能性幹細胞株の樹立に関する研究.

Degree: 博士(農学), 2016, Kyoto University / 京都大学

URL: http://hdl.handle.net/2433/215965 ; http://dx.doi.org/10.14989/doctor.k19899

新制・課程博士

甲第19899号

農博第2182号

Subjects/Keywords: iPS cells; cattle; pluripotency; trophoblast; genetically modified animals; piggyBac

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Kawaguchi, T. (2016). Studies on induction of pluripotency in bovine somatic cells and generation of induced pluripotent stem cells : ウシ体細胞の多能性誘導と人工多能性幹細胞株の樹立に関する研究. (Thesis). Kyoto University / 京都大学. Retrieved from http://hdl.handle.net/2433/215965 ; http://dx.doi.org/10.14989/doctor.k19899

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Kawaguchi, Takamasa. “Studies on induction of pluripotency in bovine somatic cells and generation of induced pluripotent stem cells : ウシ体細胞の多能性誘導と人工多能性幹細胞株の樹立に関する研究.” 2016. Thesis, Kyoto University / 京都大学. Accessed March 09, 2021. http://hdl.handle.net/2433/215965 ; http://dx.doi.org/10.14989/doctor.k19899.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Kawaguchi, Takamasa. “Studies on induction of pluripotency in bovine somatic cells and generation of induced pluripotent stem cells : ウシ体細胞の多能性誘導と人工多能性幹細胞株の樹立に関する研究.” 2016. Web. 09 Mar 2021.

Vancouver:

Kawaguchi T. Studies on induction of pluripotency in bovine somatic cells and generation of induced pluripotent stem cells : ウシ体細胞の多能性誘導と人工多能性幹細胞株の樹立に関する研究. [Internet] [Thesis]. Kyoto University / 京都大学; 2016. [cited 2021 Mar 09]. Available from: http://hdl.handle.net/2433/215965 ; http://dx.doi.org/10.14989/doctor.k19899.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Kawaguchi T. Studies on induction of pluripotency in bovine somatic cells and generation of induced pluripotent stem cells : ウシ体細胞の多能性誘導と人工多能性幹細胞株の樹立に関する研究. [Thesis]. Kyoto University / 京都大学; 2016. Available from: http://hdl.handle.net/2433/215965 ; http://dx.doi.org/10.14989/doctor.k19899

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

23. Brederode, J.C. van. Progestins as a treatment for subinvolution of placental sites in the bitch.

Degree: 2012, Universiteit Utrecht

URL: http://dspace.library.uu.nl:8080/handle/1874/289378

► Eight postpartum bitches diagnosed with subinvolution of placental sites (SIPS) were followed in this retrospective study to investigate the efficacy of treatment with low oral… (more)

Subjects/Keywords: SIPS; megestrolacetate; medroxyprogestroneacetate; trophoblast cells; progestins

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APA (6^th Edition):

Brederode, J. C. v. (2012). Progestins as a treatment for subinvolution of placental sites in the bitch. (Masters Thesis). Universiteit Utrecht. Retrieved from http://dspace.library.uu.nl:8080/handle/1874/289378

Chicago Manual of Style (16^th Edition):

Brederode, J C van. “Progestins as a treatment for subinvolution of placental sites in the bitch.” 2012. Masters Thesis, Universiteit Utrecht. Accessed March 09, 2021. http://dspace.library.uu.nl:8080/handle/1874/289378.

MLA Handbook (7^th Edition):

Brederode, J C van. “Progestins as a treatment for subinvolution of placental sites in the bitch.” 2012. Web. 09 Mar 2021.

Vancouver:

Brederode JCv. Progestins as a treatment for subinvolution of placental sites in the bitch. [Internet] [Masters thesis]. Universiteit Utrecht; 2012. [cited 2021 Mar 09]. Available from: http://dspace.library.uu.nl:8080/handle/1874/289378.

Council of Science Editors:

Brederode JCv. Progestins as a treatment for subinvolution of placental sites in the bitch. [Masters Thesis]. Universiteit Utrecht; 2012. Available from: http://dspace.library.uu.nl:8080/handle/1874/289378

Freie Universität Berlin

24. Sudheer, Smita. Differentielle Modulation der BMP-Signalwege durch Activin/Nodal und FGF- Signalwege in der Lineage-Spezifikation von humanen embryonalen Stammzellen.

Degree: 2011, Freie Universität Berlin

URL: http://dx.doi.org/10.17169/refubium-7666

► Humane embryonale Stammzellen (hESC) können durch die Modulation von Signaltransduktionskaskaden sowohl in die embryonale als auch in die extraembryonale Linie differenziert werden. Die BMP- Signaltransduktionskaskaden… (more)

▼ Humane embryonale Stammzellen (hESC) können durch die Modulation von Signaltransduktionskaskaden sowohl in die embryonale als auch in die extraembryonale Linie differenziert werden. Die BMP- Signaltransduktionskaskaden sind bekannt dafür, eine unterstützende Wirkung auf die Differenzierung der hESCs in multiple Linien auszuüben, einschließlich der Differenzierung in Trophoblast (TE). Es konnte gezeigt werden, dass die TE Bildung aus hESCs durch BMP4, BMP2, SB431542 (einem spezifischen TGFßRI Inhibitor) oder mittels eines OCT4-Knock-Downs induziert werden kann. In der vorliegenden Studie zeigen wir erstmalig, dass die Inhibition der FGF- Signaltransduktion die Trophoblastdifferenzierung mit anschließender hCG Sekretion fördert. Zudem zeigen wir, dass die Inhibition der FGF- Signaltransduktion (-FGF) allein oder in Kombination mit einer Aktivierung der BMP-Signaltransduktion bei gleichzeitiger ACTIVIN/NODAL Inhibition (+BMP-TGF- FGF) die Differenzierung von hESCs in nicht-invasive, ßhCG-Hormon sezernierende, multinukleäre Synzytiotrophoblasten induziert. Die Wirkung der letztgenannten, kombinierten Behandlung (+BMP-TGF-FGF) von hESC ist dabei besonders effizient. Unsere Ergebnisse deuten darauf hin, dass die Aktivierung der BMPSignaltransduktion (+BMP) oder eine zusätzliche Inhibition der ACTIVIN/NODALSignaltransduktion, entweder mit oder ohne gleichzeitiger Aktivierung der FGFSignaltransduktion (+BMP-TGF oder +BMP-TGF+FGF), die Differenzierung der hESCs in Richtung embryonales Mesoderm und extraembryonalem Trophoblast unterstützt, nicht aber die des Neuroectoderms. Zusammengenommen können die Erkenntnisse dieser Studie helfen die Ereignisse der frühen Keimblatt- oder Liniensegregation der Embryogenese zu verstehen. Außerdem können wir die Bildung der Synzytiotrophoblasten, die endokrine Funktion und den Wirkstoffmetabolismus der Plazenta sowie pathologische Umstände verstehen lernen und somit die Entwicklung prä-klinischer Therapien vorantreiben. Advisors/Committee Members: w (gender), Prof. Dr. Hans Lehrach (firstReferee), Prof. Dr. Petra Knaus (furtherReferee).

Subjects/Keywords: Human embryonic stem cells; differentiation; BMP; ACTIVIN/NODAL; FGF; trophoblast; 500 Naturwissenschaften und Mathematik::570 Biowissenschaften; Biologie

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Sudheer, S. (2011). Differentielle Modulation der BMP-Signalwege durch Activin/Nodal und FGF- Signalwege in der Lineage-Spezifikation von humanen embryonalen Stammzellen. (Thesis). Freie Universität Berlin. Retrieved from http://dx.doi.org/10.17169/refubium-7666

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Sudheer, Smita. “Differentielle Modulation der BMP-Signalwege durch Activin/Nodal und FGF- Signalwege in der Lineage-Spezifikation von humanen embryonalen Stammzellen.” 2011. Thesis, Freie Universität Berlin. Accessed March 09, 2021. http://dx.doi.org/10.17169/refubium-7666.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Sudheer, Smita. “Differentielle Modulation der BMP-Signalwege durch Activin/Nodal und FGF- Signalwege in der Lineage-Spezifikation von humanen embryonalen Stammzellen.” 2011. Web. 09 Mar 2021.

Vancouver:

Sudheer S. Differentielle Modulation der BMP-Signalwege durch Activin/Nodal und FGF- Signalwege in der Lineage-Spezifikation von humanen embryonalen Stammzellen. [Internet] [Thesis]. Freie Universität Berlin; 2011. [cited 2021 Mar 09]. Available from: http://dx.doi.org/10.17169/refubium-7666.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Sudheer S. Differentielle Modulation der BMP-Signalwege durch Activin/Nodal und FGF- Signalwege in der Lineage-Spezifikation von humanen embryonalen Stammzellen. [Thesis]. Freie Universität Berlin; 2011. Available from: http://dx.doi.org/10.17169/refubium-7666

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

25. NANI DJUNAIDI. FORMATION AND FUNCTION OF INVASIVE SYNCYTIOTROPHOBLAST FROM HUMAN EMBRYONIC STEM CELLS.

Degree: 2014, National University of Singapore

URL: http://scholarbank.nus.edu.sg/handle/10635/53803

Subjects/Keywords: Embryonic stem cells; Trophoblast; Placenta; Human Implantation

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

DJUNAIDI, N. (2014). FORMATION AND FUNCTION OF INVASIVE SYNCYTIOTROPHOBLAST FROM HUMAN EMBRYONIC STEM CELLS. (Thesis). National University of Singapore. Retrieved from http://scholarbank.nus.edu.sg/handle/10635/53803

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

DJUNAIDI, NANI. “FORMATION AND FUNCTION OF INVASIVE SYNCYTIOTROPHOBLAST FROM HUMAN EMBRYONIC STEM CELLS.” 2014. Thesis, National University of Singapore. Accessed March 09, 2021. http://scholarbank.nus.edu.sg/handle/10635/53803.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

DJUNAIDI, NANI. “FORMATION AND FUNCTION OF INVASIVE SYNCYTIOTROPHOBLAST FROM HUMAN EMBRYONIC STEM CELLS.” 2014. Web. 09 Mar 2021.

Vancouver:

DJUNAIDI N. FORMATION AND FUNCTION OF INVASIVE SYNCYTIOTROPHOBLAST FROM HUMAN EMBRYONIC STEM CELLS. [Internet] [Thesis]. National University of Singapore; 2014. [cited 2021 Mar 09]. Available from: http://scholarbank.nus.edu.sg/handle/10635/53803.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

DJUNAIDI N. FORMATION AND FUNCTION OF INVASIVE SYNCYTIOTROPHOBLAST FROM HUMAN EMBRYONIC STEM CELLS. [Thesis]. National University of Singapore; 2014. Available from: http://scholarbank.nus.edu.sg/handle/10635/53803

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Utah State University

26. Parasar, Parveen. Determination of the Expression Patterns of Bovine Non-Classical Major Histocompatibility Complex (MHC) Class I Proteins.

Degree: PhD, Animal, Dairy, and Veterinary Sciences, 2013, Utah State University

URL: https://digitalcommons.usu.edu/etd/2000

► My dissertation hypothesis is that bovine trophoblast cells express cell-surface and secreted non-classical major histocompatibility complex class I (MHC-Ib) proteins which inhibit NK cells… (more)

▼ My dissertation hypothesis is that bovine trophoblast cells express cell-surface and secreted non-classical major histocompatibility complex class I (MHC-Ib) proteins which inhibit NK cells and other leukocytes by binding to inhibitory receptors (e.g., LILRB1, LILRB2, KIR2DL4, and/or CD94/NKG2A). Extremely polymorphic and ubiquitously expressed classical MHC class I (MHC-Ia) proteins, which present foreign antigenic peptides to CD8+ T lymphocytes, are involved in acceptance or rejection of tissue grafts. Non-classical MHC class I (MHC-Ib) glycoproteins, such as Human Leukocyte Antigen-G (HLA-G) and murine Qa-2, are important modulators of the maternal immune system during pregnancy. MHC-Ib proteins are: (a) oligomorphic or monomorphic, (b) expressed in specific tissues under specific condtions, and (c) produced as surface and/or soluble isoforms due to alternative splicing. Third trimester-bovine trophoblast cells express both MHC-Ia and MHC-Ib proteins. The MHC-Ib proteins expressed by trophoblast cells during the third trimester of pregnancy are encoded by four bovine leukocyte antigen (BoLA) loci: BoLA-NC1, BoLA-NC2, BoLA-NC3, and BoLA-NC4. Two MHC-Ia (N*01701 and N*01802) and three MHC-Ib (NC1*00501, NC3*00101 and NC4*00201) proteins showed cell-surface expression in transfection studies performed in murine P815 and human K562 cells. Two additional isoforms, NC1*00401 and NC2*00102, were not detected on the surface of these cells. Nevertheless, both class Ia proteins, N*01701 and N*01802, and five class Ib proteins, NC1*00401, NC1*00501, NC2*00102, NC3*00101, and NC4*00201, were detected in crude cell lysates on Western blots. Precipitation of proteins from culture supernatants showed that cell-surface MHC-Ia (N*01701 and N*01802) and MHC-Ib proteins (NC1*00501, NC3*00101, and NC4*00201) are shed from the surface of these cells into the media. The mechanism of shedding of these proteins is, however, not known. Monoclonal antibodies W6/32, IL-A88, H1A, H6A, H11A, H58A, and PT-85A recognized surface MHC-I isoforms with varying affinity. We were able to develop a sandwich enzyme-linked immunosorbent assay (ELISA) using either H1A or IL-A88 antibody as the capture antibody and the W6/32 antibody for detection. We produced monoclonal antibodies against cattle NC1*00501 and NC3*00101 proteins. One monoclonal antibody generated against BoLA-NC3*00101 was highly specific. Unfortunately, due to failure to clone the NC3*00101- hybridoma, we no longer have an infinite source of this monoclonal antibody for NC3*00101. We eluted peptides from NC3*00101-transfected MHC-null K562 cells and identified peptides using liquid chromatography-mass spectrum (LC-MS) analysis. Analysis of peptide binding data using the SAS Proc mixed statistical program, suggested that the peptide EVTNQLVVL is a potential peptide ligand, which can be used to make tetramers for enumeration of antigen-specific leukocytes. Advisors/Committee Members: Christopher Davies, Gregory J. Podgorski, Kenneth L. White, ;.

Subjects/Keywords: non-classical major histocompatibility complex class I; expression patterns; bovine; trophoblast cells; sandwich enzyme-linked immunosorbent assay; Animal Sciences; Dairy Science

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Parasar, P. (2013). Determination of the Expression Patterns of Bovine Non-Classical Major Histocompatibility Complex (MHC) Class I Proteins. (Doctoral Dissertation). Utah State University. Retrieved from https://digitalcommons.usu.edu/etd/2000

Chicago Manual of Style (16^th Edition):

Parasar, Parveen. “Determination of the Expression Patterns of Bovine Non-Classical Major Histocompatibility Complex (MHC) Class I Proteins.” 2013. Doctoral Dissertation, Utah State University. Accessed March 09, 2021. https://digitalcommons.usu.edu/etd/2000.

MLA Handbook (7^th Edition):

Parasar, Parveen. “Determination of the Expression Patterns of Bovine Non-Classical Major Histocompatibility Complex (MHC) Class I Proteins.” 2013. Web. 09 Mar 2021.

Vancouver:

Parasar P. Determination of the Expression Patterns of Bovine Non-Classical Major Histocompatibility Complex (MHC) Class I Proteins. [Internet] [Doctoral dissertation]. Utah State University; 2013. [cited 2021 Mar 09]. Available from: https://digitalcommons.usu.edu/etd/2000.

Council of Science Editors:

Parasar P. Determination of the Expression Patterns of Bovine Non-Classical Major Histocompatibility Complex (MHC) Class I Proteins. [Doctoral Dissertation]. Utah State University; 2013. Available from: https://digitalcommons.usu.edu/etd/2000

University of Georgia

27. Shirley, Margaret Lenora. Characterization of trophoblast-like cells derived from human embryonic stem cells.

Degree: 2014, University of Georgia

URL: http://hdl.handle.net/10724/22795

► There are two in vitro models for forming trophoblast-like cells from human embryonic stem ce s (hESCs):(i) in an adherent culture treated with bone morphogenic… (more)

Subjects/Keywords: Human Embryonic Stem Cells (hESCs); Bone Morphogenic Protien 4 (BMP4); Embryoid Bodies (EBs); Trophoblast; Side-Chain Cleavage Enzyme (CYP11A1); Aromatase (CYP19A1).

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Shirley, M. L. (2014). Characterization of trophoblast-like cells derived from human embryonic stem cells. (Thesis). University of Georgia. Retrieved from http://hdl.handle.net/10724/22795

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Shirley, Margaret Lenora. “Characterization of trophoblast-like cells derived from human embryonic stem cells.” 2014. Thesis, University of Georgia. Accessed March 09, 2021. http://hdl.handle.net/10724/22795.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Shirley, Margaret Lenora. “Characterization of trophoblast-like cells derived from human embryonic stem cells.” 2014. Web. 09 Mar 2021.

Vancouver:

Shirley ML. Characterization of trophoblast-like cells derived from human embryonic stem cells. [Internet] [Thesis]. University of Georgia; 2014. [cited 2021 Mar 09]. Available from: http://hdl.handle.net/10724/22795.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Shirley ML. Characterization of trophoblast-like cells derived from human embryonic stem cells. [Thesis]. University of Georgia; 2014. Available from: http://hdl.handle.net/10724/22795

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

28. Kawaguchi, Takamasa. Studies on induction of pluripotency in bovine somatic cells and generation of induced pluripotent stem cells .

Degree: 2016, Kyoto University

URL: http://hdl.handle.net/2433/215965

Subjects/Keywords: iPS cells; cattle; pluripotency; trophoblast; genetically modified animals; piggyBac

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APA (6^th Edition):

Kawaguchi, T. (2016). Studies on induction of pluripotency in bovine somatic cells and generation of induced pluripotent stem cells . (Thesis). Kyoto University. Retrieved from http://hdl.handle.net/2433/215965

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Kawaguchi, Takamasa. “Studies on induction of pluripotency in bovine somatic cells and generation of induced pluripotent stem cells .” 2016. Thesis, Kyoto University. Accessed March 09, 2021. http://hdl.handle.net/2433/215965.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Kawaguchi, Takamasa. “Studies on induction of pluripotency in bovine somatic cells and generation of induced pluripotent stem cells .” 2016. Web. 09 Mar 2021.

Vancouver:

Kawaguchi T. Studies on induction of pluripotency in bovine somatic cells and generation of induced pluripotent stem cells . [Internet] [Thesis]. Kyoto University; 2016. [cited 2021 Mar 09]. Available from: http://hdl.handle.net/2433/215965.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Kawaguchi T. Studies on induction of pluripotency in bovine somatic cells and generation of induced pluripotent stem cells . [Thesis]. Kyoto University; 2016. Available from: http://hdl.handle.net/2433/215965

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Michigan State University

29. Carey, Timothy Sean. Elucidation of BRG1-dependent mechanisms that govern pluripotency gene expression in embryonic stem cells and the trophoblast lineage.

Degree: 2015, Michigan State University

URL: http://etd.lib.msu.edu/islandora/object/etd:3541

►

Thesis Ph. D. Michigan State University. Biochemistry and Molecular Biology 2015

Brahma-related gene 1 (BRG1), a chromatin remodeling ATPase, is known to function as a… (more)

▼

Thesis Ph. D. Michigan State University. Biochemistry and Molecular Biology 2015

Brahma-related gene 1 (BRG1), a chromatin remodeling ATPase, is known to function as a key regulator of gene expression eliciting both activator and repressor functions within different cell types. In pluripotent embryonic stem cells (ESCs), BRG1 is found at key regulatory elements of pluripotency genes and functions as a negative regulator to govern lineage determination. However, the underlying mechanisms by which BRG1 regulates pluripotency genes in ESCs and the trophoblast lineage are largely unknown. To elucidate the BRG1-dependent mechanisms that regulate pluripotency during early embryonic development I used a combination of mouse preimplantation embryos and CDX2-inducible ESCs that transdifferentiate into trophoblast-like cells. The cell line allowed for biochemical experiments to be performed that required large amounts of biological material to uncover mechanisms that could then be verified in the embryo.In the first experimental study of my dissertation I demonstrated that a series of dynamic transcriptional and epigenetic changes occurred at the Nanog and Oct4/Pou5f1 proximal and distal enhancer regions during trophoblast lineage development. Initially, CDX2 was recruited to Nanog and Oct4 enhancers and colocalized with BRG1. Next, OCT4 and RNA polymerase II (RNAPII) were lost and major changes in chromatin structure occurred. Histone H3 lysine 9 and lysine 14 acetylation (H3K9/14Ac) were significantly reduced and p300 and histone deacetylase 1 (HDAC1) were lost at these genes. These changes were accompanied by an increase in nucleosome occupancy as assayed by chromatin accessibility and total histone H3 chromatin immunoprecipitation (ChIP) experiments. Lastly, I showed that DNA methylation at these regulatory regions was a final step accompanying Nanog and Oct4 silencing in the trophoblast lineage. The results of these early experiments provided an epigenetic framework for subsequent functional experiments that resolved the role of BRG1 in pluripotency and trophoblast lineage development.In the second experimental study of my dissertation I examined the biological role of BRG1 in pluripotency gene regulation and trophoblast lineage development. To accomplish this a series of experiments were performed in preimplantation embryos and CDX2-inducible ESCs. First, I demonstrated that BRG1 antagonizes histone H3K9/14 acetylation at the Nanog proximal enhancer in both pluripotent ESCs and the trophoblast lineage. To understand how BRG1 regulates H3K9/14 acetylation a series of biochemical experiments were performed. I discovered that BRG1 forms a functional deacetylation complex with histone deacetylase 1 (HDAC1) in ESCs and preimplantation embryos. An important observation obtained from the embryo study was that the interaction of BRG1 with HDAC1 occurred at a higher frequency in the trophoblast lineage than in the inner cell mass (ICM). In agreement with a role in transcriptional repression, inhibition of HDAC1 resulted in an…

Advisors/Committee Members: Knott, Jason G., Floer, Monique, Henry, R. William, Arnosti, David N., Smith, George W..

Subjects/Keywords: Embryonic stem cells – Genetic aspects; Trophoblast – Genetic aspects; Gene expression; Genetic regulation; Biochemistry; Cellular biology; Developmental biology

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APA (6^th Edition):

Carey, T. S. (2015). Elucidation of BRG1-dependent mechanisms that govern pluripotency gene expression in embryonic stem cells and the trophoblast lineage. (Thesis). Michigan State University. Retrieved from http://etd.lib.msu.edu/islandora/object/etd:3541

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Carey, Timothy Sean. “Elucidation of BRG1-dependent mechanisms that govern pluripotency gene expression in embryonic stem cells and the trophoblast lineage.” 2015. Thesis, Michigan State University. Accessed March 09, 2021. http://etd.lib.msu.edu/islandora/object/etd:3541.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Carey, Timothy Sean. “Elucidation of BRG1-dependent mechanisms that govern pluripotency gene expression in embryonic stem cells and the trophoblast lineage.” 2015. Web. 09 Mar 2021.

Vancouver:

Carey TS. Elucidation of BRG1-dependent mechanisms that govern pluripotency gene expression in embryonic stem cells and the trophoblast lineage. [Internet] [Thesis]. Michigan State University; 2015. [cited 2021 Mar 09]. Available from: http://etd.lib.msu.edu/islandora/object/etd:3541.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Carey TS. Elucidation of BRG1-dependent mechanisms that govern pluripotency gene expression in embryonic stem cells and the trophoblast lineage. [Thesis]. Michigan State University; 2015. Available from: http://etd.lib.msu.edu/islandora/object/etd:3541

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

30. Bellisa de Freitas Barbosa. Influência das citocinas IL-10, TGF-β1 e IFN-γ na susceptibilidade de células trofoblásticas humanas (linhagem BeWo) e células epiteliais uterinas humanas (linhagem HeLa) à infecção por Toxoplasma gondii: papel na expressão de ICAM-1, na adesão do parasito à célula hospedeira e vias de sinalização intracelulares ativadas.

Degree: 2011, Federal University of Uberlândia

URL: http://www.bdtd.ufu.br//tde_busca/arquivo.php?codArquivo=3920

► Toxoplasma gondii é um protozoário parasito intracelular obrigatório capaz de infectar uma diversidade de células, incluindo células trofoblásticas. Estudos prévios demonstraram que interleucina (IL)-10, fator… (more)

▼ Toxoplasma gondii é um protozoário parasito intracelular obrigatório capaz de infectar uma diversidade de células, incluindo células trofoblásticas. Estudos prévios demonstraram que interleucina (IL)-10, fator transformador de crescimento (TGF)-β1 e interferon (IFN)-γ são importantes citocinas envolvidas na susceptibilidade de células trofoblásticas humanas (linhagem BeWo) e células epiteliais uterinas humanas (linhagem HeLa) à infecção por T. gondii. Adicionalmente, T. gondii é capaz de aderir à membrana plasmática de células hospedeiras através da molécula de adesão intercelular tipo 1 (ICAM-1). O presente estudo investigou dois objetivos gerais: (i) verificar o papel de IL-10, TGF-β1 e IFN-γ na expressão de ICAM-1 em células BeWo e HeLa, bem como analisar a influência de ICAM-1 na adesão de T. gondii a estas células quando tratadas ou não com as mesmas citocinas; e (ii) verificar os mecanismos intracelulares ativados por IL-10, TGF-β1 e IFN-γ em células BeWo e HeLa no intuito de compreender os efeitos diferenciais exercidos por essas citocinas nessas distintas linhagens celulares. Para executar o primeiro objetivo, células BeWo e HeLa foram infectadas por T. gondii (cepa RH) e tratadas com rIL-10, rTGF-β1 ou rIFN-γ. As células foram analisadas quanto ao índice de infecção, expressão de ICAM-1, produção de fator de necrose tumoral (TNF)-α e quanto ao papel de ICAM-1 na adesão de T. gondii às células na presença ou ausência de anticorpo neutralizante específico anti-ICAM-1. Para desenvolver o segundo objetivo, células BeWo e HeLa foram tratadas com rIL-10, rTGF-β1 ou rIFN-γ, infectadas por T. gondii (cepa 2F1 ou RH) e analisadas quanto ao índice de proliferação intracelular do parasito, à produção de citocinas, à fosforilação dos transdutores de sinais e ativadores de transcrição (STAT)-1, STAT-3 e Smad2/3, à expressão do fator regulador de interferon (IRF)- 1, bem como o efeito da inibição destas vias intracelulares na proliferação de T. gondii. Nas células BeWo, rIL-10 e rTGF-β1 induziram aumento do índice de infecção, mas apenas rTGF-β1 e rIFN-γ promoveram aumento da expressão de ICAM-1, da produção de TNF-α, do número de células ICAM-1+ com parasitos aderidos à membrana e do número total de parasitos aderidos à membrana em células ICAM-1+. Por outro lado, rTGF-β1 e rIFN-γ reduziram a expressão de ICAM-1 induzida por T. gondii em células HeLa, direcionando a uma menor susceptibilidade destas células à infecção. E, contrariamente ao observado para células HeLa, o índice de proliferação intracelular de T. gondii foi aumentado em células BeWo tratadas com rIL-10 e rTGF-β1, enquanto que rIFN-γ foi incapaz de controlar este índice nestas células. A proliferação do parasito regulada por rIFN-γ e rIL-10 em células BeWo e HeLa esteve relacionada a maior fosforilação de STAT-1 e expressão de IRF-1 em células HeLa e a fosforilação de STAT-3 em células BeWo. A influência dessas vias intracelulares na proliferação de T. gondii… Advisors/Committee Members: Neide Maria da Silva, Jose Roberto Mineo, Eloisa Amália Vieira Ferro, Deise Aparecida de Oliveira Silva, Olindo Assis Martins Filho, Míriam Rubio Faria.

Subjects/Keywords: Células trofoblásticas BeWo; Células HeLa; Toxoplasma gondii; ICAM-1; TGF-β; 1; IFN-γ;

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Barbosa, B. d. F. (2011). Influência das citocinas IL-10, TGF-β1 e IFN-γ na susceptibilidade de células trofoblásticas humanas (linhagem BeWo) e células epiteliais uterinas humanas (linhagem HeLa) à infecção por Toxoplasma gondii: papel na expressão de ICAM-1, na adesão do parasito à célula hospedeira e vias de sinalização intracelulares ativadas. (Thesis). Federal University of Uberlândia. Retrieved from http://www.bdtd.ufu.br//tde_busca/arquivo.php?codArquivo=3920

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Barbosa, Bellisa de Freitas. “Influência das citocinas IL-10, TGF-β1 e IFN-γ na susceptibilidade de células trofoblásticas humanas (linhagem BeWo) e células epiteliais uterinas humanas (linhagem HeLa) à infecção por Toxoplasma gondii: papel na expressão de ICAM-1, na adesão do parasito à célula hospedeira e vias de sinalização intracelulares ativadas.” 2011. Thesis, Federal University of Uberlândia. Accessed March 09, 2021. http://www.bdtd.ufu.br//tde_busca/arquivo.php?codArquivo=3920.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Barbosa, Bellisa de Freitas. “Influência das citocinas IL-10, TGF-β1 e IFN-γ na susceptibilidade de células trofoblásticas humanas (linhagem BeWo) e células epiteliais uterinas humanas (linhagem HeLa) à infecção por Toxoplasma gondii: papel na expressão de ICAM-1, na adesão do parasito à célula hospedeira e vias de sinalização intracelulares ativadas.” 2011. Web. 09 Mar 2021.

Vancouver:

Barbosa BdF. Influência das citocinas IL-10, TGF-β1 e IFN-γ na susceptibilidade de células trofoblásticas humanas (linhagem BeWo) e células epiteliais uterinas humanas (linhagem HeLa) à infecção por Toxoplasma gondii: papel na expressão de ICAM-1, na adesão do parasito à célula hospedeira e vias de sinalização intracelulares ativadas. [Internet] [Thesis]. Federal University of Uberlândia; 2011. [cited 2021 Mar 09]. Available from: http://www.bdtd.ufu.br//tde_busca/arquivo.php?codArquivo=3920.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Barbosa BdF. Influência das citocinas IL-10, TGF-β1 e IFN-γ na susceptibilidade de células trofoblásticas humanas (linhagem BeWo) e células epiteliais uterinas humanas (linhagem HeLa) à infecção por Toxoplasma gondii: papel na expressão de ICAM-1, na adesão do parasito à célula hospedeira e vias de sinalização intracelulares ativadas. [Thesis]. Federal University of Uberlândia; 2011. Available from: http://www.bdtd.ufu.br//tde_busca/arquivo.php?codArquivo=3920

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Open Access Theses and Dissertations