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1. Dingels, Nicole Katherine. Oxidized soybean oil alters the expression of PPAR gamma and target genes in 3T3-L1 cells.

Degree: MS, Nutrition, 2012, Georgia State University

Background: The typical western diet contains foods with modest amounts of lipid oxidation products. Previous work by us and others have demonstrated that mildly oxidized lipids promote a gain in fat mass while highly oxidized lipids decrease fat mass in rodents and triglyceride (TAG) accumulation in 3T3-L1 cells. Adipocyte differentiation is regulated by a key nuclear transcription factor known as PPARγ. Objective: To investigate if the alterations in triglyceride accumulation in 3T3-L1 cells pretreated with oxidized soy oil are due to 1) a change in PPARg DNA interactions 2) changes in the expression of SREBP-1c, PPARg, and/or its target genes. Main Methods: Confluent 3T3-L1 cells were pretreated for 24hours with 0.01% soy oil (SO) which was either unheated (unheated SO) or heated for 3, (3h-SO), 6 (6h-SO), or 9hours (9h-SO). The effect of 24hour soy oil exposure was assessed at several time points throughout the differentiation process. Alterations in PPARg DNA interaction was assessed using a PPARγ transcription factor assay kit while alterations in the expression of genes upstream and downstream of PPARγ was determined by RT-PCR. Primary and secondary products of oxidation within the SO were determined by spectrophotometry. Results: The 6hr-SO contained the greatest concentration of peroxides whereas both the 6hr-SO and 9hr-SO contained a significantly higher concentration of conjugated dienes and aldehydes.Nuclear extracts from 3T3-L1 cells pretreated with 6h-SO demonstrated the greatest reduction in PPARγ DNA binding. Compared to the unheated SO and mildly oxidized 3h-SO, cells treated with the 6h-SO had a significant reduction in SREBP-1c, PPARg, LPL, and GLUT4 expression occurring early in the differentiation process. Variations in the gene expression of 6hr-SO pretreated cells persisted within partially differentiated and mature adipocytes. Conclusions: Pre-treatment of preadipocytes with soy oil heated for ³ 6h greatly decreases the activity of PPARγ in the nucleus and adipogenic gene expression . These changes seen in early differentiation seem to correlate the best with the phenotype of reduced triglyceride accumulation seen in mature adipocytes. Advisors/Committee Members: Meera Penumetcha, Nalini Santanam, Barbara Hopkins.

Subjects/Keywords: PPAR gamma; oxidized lipids; gene expression; adipocyte differentiation; triglyceride accumulation; 3T3-L1 cells

triglyceride accumulation (TAG), insulin sensitivity, and the inflammatory response. A… …antagonist is known to inhibit adipogenesis and triglyceride accumulation. Treatment with… …regulatory enhancer binding protein-1c TAG: triglyceride TZDs: thiazolidinediones 3h-SO: soybean… …previously demonstrated a noteworthy interaction between TAG accumulation within 3T3-L1 cells and… …reduction in TAG accumulation at the end of the differentiation process. The 5 differentiation… 

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APA (6th Edition):

Dingels, N. K. (2012). Oxidized soybean oil alters the expression of PPAR gamma and target genes in 3T3-L1 cells. (Thesis). Georgia State University. Retrieved from https://scholarworks.gsu.edu/nutrition_theses/40

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16th Edition):

Dingels, Nicole Katherine. “Oxidized soybean oil alters the expression of PPAR gamma and target genes in 3T3-L1 cells.” 2012. Thesis, Georgia State University. Accessed January 19, 2021. https://scholarworks.gsu.edu/nutrition_theses/40.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7th Edition):

Dingels, Nicole Katherine. “Oxidized soybean oil alters the expression of PPAR gamma and target genes in 3T3-L1 cells.” 2012. Web. 19 Jan 2021.

Vancouver:

Dingels NK. Oxidized soybean oil alters the expression of PPAR gamma and target genes in 3T3-L1 cells. [Internet] [Thesis]. Georgia State University; 2012. [cited 2021 Jan 19]. Available from: https://scholarworks.gsu.edu/nutrition_theses/40.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Dingels NK. Oxidized soybean oil alters the expression of PPAR gamma and target genes in 3T3-L1 cells. [Thesis]. Georgia State University; 2012. Available from: https://scholarworks.gsu.edu/nutrition_theses/40

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

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