subject:(transposon mediated gene transfer)

1. Bire, Solenne. Optimisation de la biosécurité du vecteur transposon piggyBac pour le transfert de gène : utilisation des ARN messagers et des insulateurs. : Optimization of the biosafety of the piggyBac transposon for gene transfer using mRNA and insulators.

Degree: Docteur es, Sciences de la vie, 2011, Université François-Rabelais de Tours

URL: http://www.theses.fr/2011TOUR4043

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Les progrès en biotechno]ogie ont permis le développement d’outils pour le transfert de gène intégratif en transgénèse, bioproduction et thérapie génique. Cependant, trois challenges majeurs… (more)

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Les progrès en biotechno]ogie ont permis le développement d’outils pour le transfert de gène intégratif en transgénèse, bioproduction et thérapie génique. Cependant, trois challenges majeurs doivent être relevés pour garantir un système sécurisé : l’innocuité et l’efficacité du transfert, l’intégration ciblée et contrôlée dans le génome, le niveau et la durée d’expression du transgène au cours du temps. Dans ce but, mes travaux de thèse ont consisté à tester des solutions pour améliorer la biosécurité du transposon piggyBac qui nécessite un plasmide porteur du gène d’intérêt à insérer dans le génome et une source de transposase catalysant la réaction d’intégration du transgène. Une des stratégies de ma thèse repose sur l’apport de la source de transposase sous forme d’ARN messager au lieu d’ADN afin d’améliorer la stabilité de l’intégration et de réduire les effets génotoxiques en limitant la transposase dans les cellules. Pour la première fois, la biodisponibilité de l’ARNm de la transposase et les conditions optimales d’utilisation en cellules humaines ont été déterminées pour augmenter la biosêcurité du système. Le second objectif de mes travaux consiste à améliorer l’expression du transgène en ajoutant des insulateurs connus pour s’opposer à l’extinction de l’expression des gênes. En termes de biosécurité, cette stratégie permet de réduire le nombre de copies du transgène nécessaires pour obtenir une expression suffisante. Deux candidats ont été identifiés pour améliorer l’expression du transgène. La combinaison des approches ARNrn et insulateurs est prometteuse pour sécuriser le transfert de gène médié par piggyBac et pour maintenir l’expression du gène d’intérêt.

Advances in biotechnology have enabled the development of tools for gene transfer applicable to transgenesis, bioproduction and gene therapy. But, 3 major challenges must be met to ensure a secure system: the safety and effectiveness of the transfer. the targeted and controlled integration into the genome. and the level of transgene expression over time. In this aim, my thesis project was to validate solutions to improve the biosafety of the piggyBac transposon, which requires a plasmid carrying the gene of interest to be inserted in the genome, and a source of transposase which catalyzes the transgene integration. One approach of my thesis work is to deliver the source of piggyBac transposase as an mRNA molecule instead of DNA. This strategy aims to improve the stability of the integration and reduce the genotoxic effects by limiting the transposase in the cells. For the 1st time, the bioavailability of the transposase rnRNA and the optimal conditions for its use in human cells were determined to increase the biosafety of the transposon system. The 2nd objective ofmy project is to improve the expression of the transgene by adding insulators known to counteract the transgene silencing. This strategy reduces the number of integrations required ta get a sufficient expression of the transgene and thus, improve biosecurity. Two candidates have been…

Advisors/Committee Members: Rouleux-Bonnin, Florence (thesis director).

Subjects/Keywords: Transposon PiggyBac; Gene transfer; Biosafety; Transposon; PiggyBac; MRNA; Insulators

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Bire, S. (2011). Optimisation de la biosécurité du vecteur transposon piggyBac pour le transfert de gène : utilisation des ARN messagers et des insulateurs. : Optimization of the biosafety of the piggyBac transposon for gene transfer using mRNA and insulators. (Doctoral Dissertation). Université François-Rabelais de Tours. Retrieved from http://www.theses.fr/2011TOUR4043

Chicago Manual of Style (16^th Edition):

Bire, Solenne. “Optimisation de la biosécurité du vecteur transposon piggyBac pour le transfert de gène : utilisation des ARN messagers et des insulateurs. : Optimization of the biosafety of the piggyBac transposon for gene transfer using mRNA and insulators.” 2011. Doctoral Dissertation, Université François-Rabelais de Tours. Accessed January 16, 2021. http://www.theses.fr/2011TOUR4043.

MLA Handbook (7^th Edition):

Bire, Solenne. “Optimisation de la biosécurité du vecteur transposon piggyBac pour le transfert de gène : utilisation des ARN messagers et des insulateurs. : Optimization of the biosafety of the piggyBac transposon for gene transfer using mRNA and insulators.” 2011. Web. 16 Jan 2021.

Vancouver:

Bire S. Optimisation de la biosécurité du vecteur transposon piggyBac pour le transfert de gène : utilisation des ARN messagers et des insulateurs. : Optimization of the biosafety of the piggyBac transposon for gene transfer using mRNA and insulators. [Internet] [Doctoral dissertation]. Université François-Rabelais de Tours; 2011. [cited 2021 Jan 16]. Available from: http://www.theses.fr/2011TOUR4043.

Council of Science Editors:

Bire S. Optimisation de la biosécurité du vecteur transposon piggyBac pour le transfert de gène : utilisation des ARN messagers et des insulateurs. : Optimization of the biosafety of the piggyBac transposon for gene transfer using mRNA and insulators. [Doctoral Dissertation]. Université François-Rabelais de Tours; 2011. Available from: http://www.theses.fr/2011TOUR4043

2. Zeng, Lu. The impact of transposable elements on amniote evolution.

Degree: 2018, University of Adelaide

URL: http://hdl.handle.net/2440/114589

► Transposable elements (TEs) are mobile DNA sequences, often called “jumping genes” because of their ability to replicate to new genomic locations. As a result, TEs… (more)

▼ Transposable elements (TEs) are mobile DNA sequences, often called “jumping genes” because of their ability to replicate to new genomic locations. As a result, TEs make up a significant proportion of the eukaryotic genomes we see today. Growing evidence suggests that TEs are catalysts of genomic change. TE insertions into regulatory regions can lead to new genes, or they can disrupt host sequences and serve as substrates for homologous recombination, generating DNA rearrangements. At the RNA level, TEs can carry transcription factor binding sites, causing alternative splicing and thus impacting gene expression. Some TEs are even capable of jumping between different genomes, using viruses or parasitic insects as transfer vectors. Originally viewed as “junk” DNA, TEs are now recognised as powerful drivers of genome evolution. However, there are numerous computational challenges to accurately detecting and characterising TEs in genomic data. Many existing tools and pipelines are designed to explicitly remove repetitive, non-unique sequences. Likewise, TE annotation software relies heavily on the use of query sequences and reference databases. This restricts the ability to find new types of TEs (or mutated forms of known TEs), mainly suited to the analysis of model organisms such as human and mouse. In this thesis, I describe an ab initio pipeline for identifying species-specific repeats and segmental duplications with high sensitivity and accuracy. I consider a repeat in the truest sense of the word: any sequence that appears more than once in the genome. Using eight representative species from each branch of amniote evolution, I use this novel method to portray the remarkable diversity of TEs across species and trace different repeats to their families and consensus sequences. Reptiles are particularly renowned for their unusual TE dynamics. In Chapter 3, I investigate TE evolution in the tuatara genome: a New Zealand reptile. The tuatara is the only surviving member of its order, which flourished around 200 million years ago. Its most recent common ancestor with any other extant group is the lizards and snakes. The tuatara is therefore of great interest to evolutionary geneticists. In most reptiles, CR1 repeats make up the dominant TE class. My results indicate that the tuatara genome is distinct from other reptiles because the two most dominant TE families are L2 and MIR elements. Furthermore, I describe a likely transfer of L2 elements between tuatara and monotremes (platypus and echidna), potentially explaining the predominance of L2s in the tuatara genome. In Chapter 4, I extend my TE analysis to consider gene expression in six species. Due to the prevalence of TEs in the genome, I used a bootstrap approach to minimize the co-occurrence of multiple TE types in one gene. My results show that species-specific associations of TEs with gene expression support a role for TEs in speciation/response to selection by species. Altogether, this thesis presents novel and ab initio… Advisors/Committee Members: Adelson, David Louis (advisor), Kortschak, Daniel (advisor), School of Biological Sciences (school).

Subjects/Keywords: Research by publication; transposon; amniote; evolution; gene expression; retrotransposon; horizontal transfer

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APA (6^th Edition):

Zeng, L. (2018). The impact of transposable elements on amniote evolution. (Thesis). University of Adelaide. Retrieved from http://hdl.handle.net/2440/114589

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Zeng, Lu. “The impact of transposable elements on amniote evolution.” 2018. Thesis, University of Adelaide. Accessed January 16, 2021. http://hdl.handle.net/2440/114589.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Zeng, Lu. “The impact of transposable elements on amniote evolution.” 2018. Web. 16 Jan 2021.

Vancouver:

Zeng L. The impact of transposable elements on amniote evolution. [Internet] [Thesis]. University of Adelaide; 2018. [cited 2021 Jan 16]. Available from: http://hdl.handle.net/2440/114589.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Zeng L. The impact of transposable elements on amniote evolution. [Thesis]. University of Adelaide; 2018. Available from: http://hdl.handle.net/2440/114589

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Leiden University

3. Mezzadra, R. Genetic manipulation and genetic-based dissection of tumor-specific immunity.

Degree: 2019, Leiden University

URL: http://hdl.handle.net/1887/68811

► In this thesis I have firstly applied gene transfer technologies to the redirection of T cell specificity, by trying to overcome limitations related to non-viral… (more)

Subjects/Keywords: PD-L1; T cells; genetic screens; transposon-mediated gene-transfer; SLFN11; IFN-gamma; TCR; PD-L1; T cells; genetic screens; transposon-mediated gene-transfer; SLFN11; IFN-gamma; TCR

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Mezzadra, R. (2019). Genetic manipulation and genetic-based dissection of tumor-specific immunity. (Doctoral Dissertation). Leiden University. Retrieved from http://hdl.handle.net/1887/68811

Chicago Manual of Style (16^th Edition):

Mezzadra, R. “Genetic manipulation and genetic-based dissection of tumor-specific immunity.” 2019. Doctoral Dissertation, Leiden University. Accessed January 16, 2021. http://hdl.handle.net/1887/68811.

MLA Handbook (7^th Edition):

Mezzadra, R. “Genetic manipulation and genetic-based dissection of tumor-specific immunity.” 2019. Web. 16 Jan 2021.

Vancouver:

Mezzadra R. Genetic manipulation and genetic-based dissection of tumor-specific immunity. [Internet] [Doctoral dissertation]. Leiden University; 2019. [cited 2021 Jan 16]. Available from: http://hdl.handle.net/1887/68811.

Council of Science Editors:

Mezzadra R. Genetic manipulation and genetic-based dissection of tumor-specific immunity. [Doctoral Dissertation]. Leiden University; 2019. Available from: http://hdl.handle.net/1887/68811

4. Cooney, Ashley L. Integrating viral vectors as a gene therapy approach for cystic fibrosis.

Degree: PhD, Microbiology, 2018, University of Iowa

URL: https://ir.uiowa.edu/etd/6083

► Cystic fibrosis (CF) is the most common autosomal recessive genetic disease in Caucasian populations. CF affects multiple organ systems including pancreas, liver, intestines, sweat… (more)

▼ Cystic fibrosis (CF) is the most common autosomal recessive genetic disease in Caucasian populations. CF affects multiple organ systems including pancreas, liver, intestines, sweat glands, and male reproductive organs, however the leading cause of morbidity and mortality in CF patients is chronic lung disease. CF is caused by a mutant cystic fibrosis transmembrane conductance regulator (CFTR) gene which leads to chloride (Cl-) and bicarbonate (HCO3-) anion dysregulation at the airway surface. Without adequate anion exchange, thick, viscous mucus accumulates at the airway surface allowing bacterial colonization to occur. Complementing CFTR in the appropriate airway cells restores the anion channel activity in CFTR-deficient cells. The ultimate goal for CF gene therapy is to design an integrating vector that would lead to persistent and efficient expression of CFTR in the airways. Performing gene therapy experiments is dependent upon a relevant animal model. The CF pig is a large animal model similar in size, anatomy, and physiology to humans. Importantly, the CF pig recapitulates human lung disease. From the CF pig, we have learned much about CF lung disease and have developed relevant assays to measure anion channel correction. We have learned that loss of CFTR leads to a decreased airway surface ASL pH, bacterial killing ability, and increased mucus viscosity. Standardized assays have been developed to evaluate the change in current by Ussing chambers, ASL pH, bacterial killing in vivo and ASL pH and viscosity on primary airway cultures in vitro. Ultimately, these metrics allow us to make conclusions about the efficiency of CFTR restoration. Viral vectors are promising candidates for CF gene therapy. Viral vectors such as adenovirus (Ad), adeno-associated virus (AAV), and pseudotyped lentiviral vectors such as feline immunodeficiency virus (FIV) or human immunodeficiency virus (HIV) can efficiently transduce airway cells and express CFTR. Ad and AAV have both been tested in CF clinical trials, but CFTR expression was transient, if detected at all. Understanding vector biology and overcoming barriers in the lung have allowed us to improve vector delivery to the airways. However, the next major hurdle was achieving persistent expression. Ad and AAV are both transiently expressing vectors, and vector readministration is implausible due to the presence of neutralizing antibodies that develop against the vector. Creating a hybrid nonviral/viral vector in which the integrating nonviral piggyBac transposon system is delivered… Advisors/Committee Members: Sinn, Patrick L. (supervisor).

Subjects/Keywords: CF pig; cystic fibrosis; gene therapy; gene transfer; piggyBac transposon; viral vectors; Microbiology

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APA (6^th Edition):

Cooney, A. L. (2018). Integrating viral vectors as a gene therapy approach for cystic fibrosis. (Doctoral Dissertation). University of Iowa. Retrieved from https://ir.uiowa.edu/etd/6083

Chicago Manual of Style (16^th Edition):

Cooney, Ashley L. “Integrating viral vectors as a gene therapy approach for cystic fibrosis.” 2018. Doctoral Dissertation, University of Iowa. Accessed January 16, 2021. https://ir.uiowa.edu/etd/6083.

MLA Handbook (7^th Edition):

Cooney, Ashley L. “Integrating viral vectors as a gene therapy approach for cystic fibrosis.” 2018. Web. 16 Jan 2021.

Vancouver:

Cooney AL. Integrating viral vectors as a gene therapy approach for cystic fibrosis. [Internet] [Doctoral dissertation]. University of Iowa; 2018. [cited 2021 Jan 16]. Available from: https://ir.uiowa.edu/etd/6083.

Council of Science Editors:

Cooney AL. Integrating viral vectors as a gene therapy approach for cystic fibrosis. [Doctoral Dissertation]. University of Iowa; 2018. Available from: https://ir.uiowa.edu/etd/6083

5. Perales, Jose Carlos. Receptor-mediated gene transfer in vivo.

Degree: PhD, Biochemistry, 1995, Case Western Reserve University School of Graduate Studies

URL: http://rave.ohiolink.edu/etdc/view?acc_num=case1057931585

► Receptor-mediated gene transfer is an attractive method for therapeutically correcting human genetic diseases since it permits the targeting of DNA to cellular receptors in specific… (more)

▼ Receptor-mediated gene transfer is an attractive method for therapeutically correcting human genetic diseases since it permits the targeting of DNA to cellular receptors in specific tissues of adult animals. The gene delivery vehicle is composed of the DNA of interest, a DNA binding moiety (i.e., poly(L-lysine)) and a ligand for the target receptor. Genes introduced by this technique have been shown to be expressed in the target tissue for varying periods. However, to be useful for gene therapy, it is critical that both the chemical properties and physical interactions of the reagents involved in the design of the DNA delivery vehicle (DNA/ligand complex) be rigorously characterized. To obtain a DNA/ligand complex suitable for in vivo gene transfer, I have developed a multi-step protocol for the interaction of poly(L-lysine) with DNA in solution. I demonstrate here that the slow increase in the concentration of poly(L-lysine) in a vortexing solution of DNA results in the formation of DNA/ligand-poly(L-lysine) particles of defined size and shape. I hypothesize that binding of poly(L-lysine) to DNA initiates condensation by forming a nucleus of condensation along the DNA length. Further addition of poly(L-lysine) results in the formation of aggregated DNA/ligand-poly(L-lysine) complexes. The second step in the formation of stable condensed DNA particles consists in equilibrating the ionic strength of the solvent to facilitate the solubilization of the condensed DNA particles. Several biochemical parameters have been studied and correlated with the functional activity of DNA/ligand-poly(L-lysine) particles in transferring genes to the liver of adult animals by receptor-mediated endocytosis. Based on these findings, a model for the interaction between the DNA and the ligand/poly(L-lysine) is proposed and the implications for the effective transfer of genes into animals in vivo discussed. The model proposed indicates a role for the polycation in both the formation of suitable particles for gene transfer, and the efficient transfer of the DNA to the nucleus of cells Advisors/Committee Members: Hanson, Richard (Advisor).

Subjects/Keywords: Receptor-mediated gene transfer

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APA (6^th Edition):

Perales, J. C. (1995). Receptor-mediated gene transfer in vivo. (Doctoral Dissertation). Case Western Reserve University School of Graduate Studies. Retrieved from http://rave.ohiolink.edu/etdc/view?acc_num=case1057931585

Chicago Manual of Style (16^th Edition):

Perales, Jose Carlos. “Receptor-mediated gene transfer in vivo.” 1995. Doctoral Dissertation, Case Western Reserve University School of Graduate Studies. Accessed January 16, 2021. http://rave.ohiolink.edu/etdc/view?acc_num=case1057931585.

MLA Handbook (7^th Edition):

Perales, Jose Carlos. “Receptor-mediated gene transfer in vivo.” 1995. Web. 16 Jan 2021.

Vancouver:

Perales JC. Receptor-mediated gene transfer in vivo. [Internet] [Doctoral dissertation]. Case Western Reserve University School of Graduate Studies; 1995. [cited 2021 Jan 16]. Available from: http://rave.ohiolink.edu/etdc/view?acc_num=case1057931585.

Council of Science Editors:

Perales JC. Receptor-mediated gene transfer in vivo. [Doctoral Dissertation]. Case Western Reserve University School of Graduate Studies; 1995. Available from: http://rave.ohiolink.edu/etdc/view?acc_num=case1057931585

University of Iowa

6. Burnight, Erin Rae. Targeting therapeutic vector expression and integration for gene therapy applications.

Degree: PhD, Genetics, 2011, University of Iowa

URL: https://ir.uiowa.edu/etd/2831

► Gene therapy is an attractive treatment for many genetic diseases because rather than treat the symptoms of the disease, it has the potential to… (more)

▼ Gene therapy is an attractive treatment for many genetic diseases because rather than treat the symptoms of the disease, it has the potential to correct the underlying defect. Cystic fibrosis and hemophilia A are two monogenic disorders that are particularly well-suited to treatment with gene therapy as a relatively small increase in the function is needed to see improvement. Gene therapy has provided some correction in both diseases using a variety of vector systems but sustained expression and long term correction have yet to be demonstrated in the clinic. It is unclear in which cell type(s) correction of the underlying defect in cystic fibrosis will be most effective. Studies indicate that the majority of CFTR expression is in the submucosal glands and ciliated epithelia – a terminally differentiated cell type (Engelhardt, J.F. et al, 2004, Journal of Clinical Investigation). Therapeutic gene transfer would thus be most effective if achieved in a progenitor cell type. Additionally, the native regulation of CFTR has not been definitively elucidated. To this end, one goal of our studies is to develop a lentiviral vector system with heterologous promoters of varying strengths and cell specificity to aid in our selection of optimal reagents for appropriate CFTR expression. We show that use of novel internal promoters from the human PLUNC and WDR65 genes direct persistent expression in the airway. Additionally, disruption of the nasal epithelium with the detergent polidocanol eliminated reporter expression in mouse airway. Two weeks post-treatment, expression returned indicating targeting of a progenitor cell population with our novel vectors. Integrating vector systems can treat chronic diseases such as cystic fibrosis because expression can persist long term from these vectors if cells with progenitor capacity are targeted (Sinn, P.L. et al, 2005, Journal of Virology). However, the potential for genotoxicity from vector-related dysregulation is a concern. Thus, a second aim of these studies was to develop a lentiviral vector that can target a specific locus in the genome. We developed a FIV vector in which the integrase was modified with a protein-binding domain that when co-delivered with a fusion consisting of the cognate protein and a DNA binding domain would tether the vector to the appropriate locus. Unfortunately, integrase modification rendered the vector catalytically inactive. Lastly, we hoped to develop a non-viral transposon vector system (piggyBac) for gene transfer applications to the liver for treatment of hemophilia A. The recent demonstration that piggyBac transposase is highly active in mammalian cells warrants further development… Advisors/Committee Members: McCray, Paul B., 1954- (supervisor).

Subjects/Keywords: gene therapy; transposon; viral vector; Genetics

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Burnight, E. R. (2011). Targeting therapeutic vector expression and integration for gene therapy applications. (Doctoral Dissertation). University of Iowa. Retrieved from https://ir.uiowa.edu/etd/2831

Chicago Manual of Style (16^th Edition):

Burnight, Erin Rae. “Targeting therapeutic vector expression and integration for gene therapy applications.” 2011. Doctoral Dissertation, University of Iowa. Accessed January 16, 2021. https://ir.uiowa.edu/etd/2831.

MLA Handbook (7^th Edition):

Burnight, Erin Rae. “Targeting therapeutic vector expression and integration for gene therapy applications.” 2011. Web. 16 Jan 2021.

Vancouver:

Burnight ER. Targeting therapeutic vector expression and integration for gene therapy applications. [Internet] [Doctoral dissertation]. University of Iowa; 2011. [cited 2021 Jan 16]. Available from: https://ir.uiowa.edu/etd/2831.

Council of Science Editors:

Burnight ER. Targeting therapeutic vector expression and integration for gene therapy applications. [Doctoral Dissertation]. University of Iowa; 2011. Available from: https://ir.uiowa.edu/etd/2831

Brunel University

7. Ouboussad, Lylia. Identification of novel tumour suppressor genes involved in the development of cutaneous malignant melanoma.

Degree: PhD, 2009, Brunel University

URL: http://bura.brunel.ac.uk/handle/2438/4393 ; http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.511685

► Skin cancer is one of the most common forms of adult solid tumour. The incidence is increasing rapidly making skin cancer a major health problem… (more)

Subjects/Keywords: 616.994; Microcell mediated chromosome transfer : Gene expression analysis

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Ouboussad, L. (2009). Identification of novel tumour suppressor genes involved in the development of cutaneous malignant melanoma. (Doctoral Dissertation). Brunel University. Retrieved from http://bura.brunel.ac.uk/handle/2438/4393 ; http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.511685

Chicago Manual of Style (16^th Edition):

Ouboussad, Lylia. “Identification of novel tumour suppressor genes involved in the development of cutaneous malignant melanoma.” 2009. Doctoral Dissertation, Brunel University. Accessed January 16, 2021. http://bura.brunel.ac.uk/handle/2438/4393 ; http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.511685.

MLA Handbook (7^th Edition):

Ouboussad, Lylia. “Identification of novel tumour suppressor genes involved in the development of cutaneous malignant melanoma.” 2009. Web. 16 Jan 2021.

Vancouver:

Ouboussad L. Identification of novel tumour suppressor genes involved in the development of cutaneous malignant melanoma. [Internet] [Doctoral dissertation]. Brunel University; 2009. [cited 2021 Jan 16]. Available from: http://bura.brunel.ac.uk/handle/2438/4393 ; http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.511685.

Council of Science Editors:

Ouboussad L. Identification of novel tumour suppressor genes involved in the development of cutaneous malignant melanoma. [Doctoral Dissertation]. Brunel University; 2009. Available from: http://bura.brunel.ac.uk/handle/2438/4393 ; http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.511685

University of Notre Dame

8. Bharath Balu. Genetic Analysis of Plasmodium Falciparum</h1>.

Degree: Biological Sciences, 2005, University of Notre Dame

URL: https://curate.nd.edu/show/9z902z12x87

► Genome sequencing of Plasmodium falciparum is almost complete but the functional analysis of the Plasmodium falciparum genome is restricted because of our limited ability… (more)

▼ Genome sequencing of Plasmodium falciparum is almost complete but the functional analysis of the Plasmodium falciparum genome is restricted because of our limited ability to genetically manipulate this malaria parasite. Gene regulation is one of the most intriguing and enigmatic aspects of malaria parasite biology. The ability of the parasite to control differential gene expression is poorly understood and so far only a few Plasmodium promoters have been identified that share no significant homology with either themselves or with other eukaryotic promoters. maebl is a paralogue of the ebl gene family in P. falciparum and is expressed at a different time point in the blood stages of the parasite as compared to the other well characterized members of the family, baebl and eba-175. Transcript analysis by northern blot hybridizations and Random amplification of cDNA ends (RACE) idenitifed a much longer 5’ untranslated region for maebl when compared to baebl and eba-175, suggesting the possibility of post-transcriptional regulation for maebl. A single transcription start site was mapped for eba-175 and multiple transcription start sites were identified for baebl and maebl. In order to test the promoter activity of the 5’ regions of baebl, eba- 175 and maebl, a modified Chloramphenicol acetyl transferase (CAT) reporter assay was developed for P. facliparum that is more accurate and user-friendly compared to the conventional CAT assays. The 5’ regions of all three genes expressed CAT from episomes in P. falciparum blood stages confirming the presence of promoter elements in 5’ regions. So far, only a few promoters are used for transgene expression in P. falciparum and our results show that the 5’ regions of these three ebl genes could be used for the same. MAEBL is expressed in the mid-trophozoite stages of blood-stage development and is located on the surface of merozoites just before the invasion of new host erythrocytes. To understand the role of MAEBL in erythrocyte invasion, maebl was disrupted in P. falciparum NF54 parasites by single homologous recombination. There was no apparent phenotypic effect of maebl disruption in these parasites. Erythrocyte invasion assays performed on different enzymatically-treated erythrocytes showed that maebl is not essential for blood-stage development and the disruptant clones had a similar invasion phenotype to that of wild-type parasites. It is possible that the function of MAEBL is complemented by another parasite protein hence masking the effect of maebl disruption. In the rodent malaria parasite, P. berghei, MAEBL has been shown to be essential for mosquito salivary gland invasion by malarial sporozoites. In future, we would like to confirm a similar role for MAEBL in P. falciparum using the maebl disruptant clones and also investigate the possible role for MAEBL in the sporozoite invasion of human liver cells. Application of new technologies has produced a wealth of information in recent years about the genomes, proteomes, and other aspects… Advisors/Committee Members: Dr. Kristin Hager, Committee Member, Dr. David Severson, Committee Member, Dr. Paul Huber, Committee Member, Dr. John H Adams, Committee Member.

Subjects/Keywords: GENE REGULATION; PLASMODIUM; MALARIA; TRANSPOSON

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APA (6^th Edition):

Balu, B. (2005). Genetic Analysis of Plasmodium Falciparum</h1>. (Thesis). University of Notre Dame. Retrieved from https://curate.nd.edu/show/9z902z12x87

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Balu, Bharath. “Genetic Analysis of Plasmodium Falciparum</h1>.” 2005. Thesis, University of Notre Dame. Accessed January 16, 2021. https://curate.nd.edu/show/9z902z12x87.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Balu, Bharath. “Genetic Analysis of Plasmodium Falciparum</h1>.” 2005. Web. 16 Jan 2021.

Vancouver:

Balu B. Genetic Analysis of Plasmodium Falciparum</h1>. [Internet] [Thesis]. University of Notre Dame; 2005. [cited 2021 Jan 16]. Available from: https://curate.nd.edu/show/9z902z12x87.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Balu B. Genetic Analysis of Plasmodium Falciparum</h1>. [Thesis]. University of Notre Dame; 2005. Available from: https://curate.nd.edu/show/9z902z12x87

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Sydney

9. Siew, Susan Mei-Ling. Recombinant AAV-mediated Gene Therapy Approaches to Treat Progressive Familial Intrahepatic Cholestasis Type 3 .

Degree: 2014, University of Sydney

URL: http://hdl.handle.net/2123/12409

► Among contemporary gene transfer vehicles, non-pathogenic recombinant adeno-associated viral vectors (rAAV) show exceptional promise for liver-targeted therapeutic approaches. The broad focus of studies described in… (more)

Subjects/Keywords: Gene therapy; Adeno-associated virus; PFIC; PiggyBac transposon; Liver disease

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Siew, S. M. (2014). Recombinant AAV-mediated Gene Therapy Approaches to Treat Progressive Familial Intrahepatic Cholestasis Type 3 . (Thesis). University of Sydney. Retrieved from http://hdl.handle.net/2123/12409

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Siew, Susan Mei-Ling. “Recombinant AAV-mediated Gene Therapy Approaches to Treat Progressive Familial Intrahepatic Cholestasis Type 3 .” 2014. Thesis, University of Sydney. Accessed January 16, 2021. http://hdl.handle.net/2123/12409.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Siew, Susan Mei-Ling. “Recombinant AAV-mediated Gene Therapy Approaches to Treat Progressive Familial Intrahepatic Cholestasis Type 3 .” 2014. Web. 16 Jan 2021.

Vancouver:

Siew SM. Recombinant AAV-mediated Gene Therapy Approaches to Treat Progressive Familial Intrahepatic Cholestasis Type 3 . [Internet] [Thesis]. University of Sydney; 2014. [cited 2021 Jan 16]. Available from: http://hdl.handle.net/2123/12409.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Siew SM. Recombinant AAV-mediated Gene Therapy Approaches to Treat Progressive Familial Intrahepatic Cholestasis Type 3 . [Thesis]. University of Sydney; 2014. Available from: http://hdl.handle.net/2123/12409

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Iowa State University

10. Su, Weijia. The impact of Terminal Inverted Repeat (TIR) transposable elements on plant genomes.

Degree: 2020, Iowa State University

URL: https://lib.dr.iastate.edu/etd/18235

► As major components in eukaryotic genomes, Transposable Elements (TEs) have been studied for decades. In the 1950s to 1990s, the predominant opinions considered TEs as… (more)

▼ As major components in eukaryotic genomes, Transposable Elements (TEs) have been studied for decades. In the 1950s to 1990s, the predominant opinions considered TEs as “junk DNA” and “selfish elements”. However, subsequent studies showed that TEs are important players in genome dynamics. TEs are presented in many shapes and characteristics in genomes. Generally, two classes of TEs are distinguished by their transposition features: Class I TEs rely on RNA for their transposition, and Class II TEs are independent of RNA intermediates. In this thesis, I focus on the major type of Class II TEs which is called Terminal Inverted Repeat (TIR) elements. While most of the TIR TEs are heavily methylated and are considered inactive, recent studies have shown that in many plant genomes some TIR TE families are still able to transpose. Besides that, in certain situations, different TEs are capable to work together to generate larger rearrangements in genomes. In this thesis, I present our findings of novel TIR activities and the tools we developed to facilitate TE studies. This thesis contains 6 chapters and these results will contribute to our knowledge of TIR TEs in plant genomes. In the first chapter, I summarize the evolutionary impacts of TIR elements when they mobilize through alternative transpositions. Unlike standard transposition, alternative transposition involves two distinct TEs and generates more complicated genome rearrangements such as deletion, inversion, duplication, and translocation. The first chapter serves as an introduction to alternative transposition. I describe various types of alternative transposition induced by TIR transposable elements and their outcomes. In the second chapter, I present our results of Composite Insertions (CIs), a novel structure generated by Ac/Ds induced alternative transposition. We used maize pericarp color 1 (p1) and pericarp color 2 (p2) genes as a genetic system to identify CIs. We analyzed their structures and proved our hypothesis that these CIs can regulate gene expression by duplicating and shuffling enhancer elements. Previous studies have shown that transposable elements perform enhancer-like activities. The mechanism behind this function is typically related to the evolutionary forces, which drive TEs evolving into regulatory elements. Also, most of these studies were focused on Class I TEs in animal and human systems. This chapter demonstrates an alternative way of how Class II TEs contribute to gene regulation in maize. To analyze the larger impact of TIR TEs, we developed a bioinformatics pipeline called TIR-Learner. It is a new ensemble method for TIR Transposable Element annotation. In chapter 3, I introduce the algorithms and methods used in TIR-Learner, and the analysis of Maize TIR elements we performed by using TIR-Learner. TIR-Learner combines the advantages of current homology-based annotation methods with the powerful de-novo machine-learning approaches, resulting in better efficiency and accuracy of TIR element annotation. By using this pipeline, we…

Subjects/Keywords: Bioinformatics Pipeline; Gene Regulation; Genome; Genome Rearrangement; TIR Transposon; Transposable Elements

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Su, W. (2020). The impact of Terminal Inverted Repeat (TIR) transposable elements on plant genomes. (Thesis). Iowa State University. Retrieved from https://lib.dr.iastate.edu/etd/18235

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Su, Weijia. “The impact of Terminal Inverted Repeat (TIR) transposable elements on plant genomes.” 2020. Thesis, Iowa State University. Accessed January 16, 2021. https://lib.dr.iastate.edu/etd/18235.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Su, Weijia. “The impact of Terminal Inverted Repeat (TIR) transposable elements on plant genomes.” 2020. Web. 16 Jan 2021.

Vancouver:

Su W. The impact of Terminal Inverted Repeat (TIR) transposable elements on plant genomes. [Internet] [Thesis]. Iowa State University; 2020. [cited 2021 Jan 16]. Available from: https://lib.dr.iastate.edu/etd/18235.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Su W. The impact of Terminal Inverted Repeat (TIR) transposable elements on plant genomes. [Thesis]. Iowa State University; 2020. Available from: https://lib.dr.iastate.edu/etd/18235

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Illinois – Urbana-Champaign

11. Waters, Jillian. Transcriptional regulation of mobilization and transfer of the Bacteroides conjugative transposon CTnDOT.

Degree: PhD, 0322, 2013, University of Illinois – Urbana-Champaign

URL: http://hdl.handle.net/2142/45596

► Bacteroides spp. are a primary inhabitant of the human colon, and play an important role in the health of the human host as Bacteroides are… (more)

▼ Bacteroides spp. are a primary inhabitant of the human colon, and play an important role in the health of the human host as Bacteroides are important for nutrient acquisition, the breakdown of starches that would otherwise go undigested, and the management of relevant pathogens such as C. difficile. Although generally considered a commensal, Bacteroides can become an opportunistic pathogen if it should escape the colon. Bacteroides is the most commonly isolated causative agent of anaerobic infections, and these infections are rather difficult to treat due to the prevalence of antibiotic resistance within this genus. The prevalence of resistance determinants in Bacteroides is often due to mobile genetic elements. One such element is a conjugative transposon (sometimes referred to as an integrative conjugative element, or ICE) called CTnDOT, which is a 65 kb ICE that encodes resistance to the antibiotics erythromycin and tetracycline. A notable feature of CTnDOT is that excision from the donor chromosome and conjugative transfer are coordinately regulated upon exposure of donor cells to tetracycline (Tc). While no transfer is detected in the absence of Tc, upon Tc induction a regulatory cascade ultimately stimulates synthesis of the excision proteins, which are required for excision of CTnDOT from the chromosome. These proteins also have a regulatory role, in that they are required for the transcriptional activation of the 13 kb tra operon that encodes the mating apparatus. The work presented in this dissertation has characterized a negative regulator, RteR, that appears to prevent conjugative transfer of CTnDOT in the absence of Tc by possibly initiating the formation of an intrinsic terminator within traB, thus truncating the transcript so there is no substrate for translation, and hence no proteins are formed to assemble the mating apparatus. For the first time, we also describe the regulation of the CTnDOT mobilization region. These three genes that encode the relaxases and coupling protein required for mobilization are organized in an approximately 4 kb operon that is regulated upon Tc induction. The excision proteins Xis2d and Exc are required for enhancement of mob transcription upon exposure to Tc. This differs from the neighboring divergently transcribed tra operon, which does not require Exc, but also requires Xis2d and in addition Xis2c. A negative regulator is preventing mob transcription in the absence of Tc, and we currently predict that a gene encoded downstream of intDOT, orf2, is the mob transcriptional repressor. Taken together, the work described in this dissertation has further shed light on the intricate regulation governing the conjugative transfer of CTnDOT. Advisors/Committee Members: Salyers, Abigail A. (advisor), Salyers, Abigail A. (Committee Chair), Orlean, Peter A. (committee member), Shisler, Joanna L. (committee member), Vanderpool, Carin K. (committee member).

Subjects/Keywords: Bacteroides; CTnDOT; conjugative transposon; conjugative transfer; mobilization; small RNA; transcriptional regulation

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Waters, J. (2013). Transcriptional regulation of mobilization and transfer of the Bacteroides conjugative transposon CTnDOT. (Doctoral Dissertation). University of Illinois – Urbana-Champaign. Retrieved from http://hdl.handle.net/2142/45596

Chicago Manual of Style (16^th Edition):

Waters, Jillian. “Transcriptional regulation of mobilization and transfer of the Bacteroides conjugative transposon CTnDOT.” 2013. Doctoral Dissertation, University of Illinois – Urbana-Champaign. Accessed January 16, 2021. http://hdl.handle.net/2142/45596.

MLA Handbook (7^th Edition):

Waters, Jillian. “Transcriptional regulation of mobilization and transfer of the Bacteroides conjugative transposon CTnDOT.” 2013. Web. 16 Jan 2021.

Vancouver:

Waters J. Transcriptional regulation of mobilization and transfer of the Bacteroides conjugative transposon CTnDOT. [Internet] [Doctoral dissertation]. University of Illinois – Urbana-Champaign; 2013. [cited 2021 Jan 16]. Available from: http://hdl.handle.net/2142/45596.

Council of Science Editors:

Waters J. Transcriptional regulation of mobilization and transfer of the Bacteroides conjugative transposon CTnDOT. [Doctoral Dissertation]. University of Illinois – Urbana-Champaign; 2013. Available from: http://hdl.handle.net/2142/45596

12. Cavalcanti, Paulo Varoni. Hibridização in situ em espermatozóides bovinos tratados com DNA exógeno: estudo experimental.

Degree: Mestrado, Reprodução Animal, 2010, University of São Paulo

URL: http://www.teses.usp.br/teses/disponiveis/10/10131/tde-02022011-211958/ ;

►

Muitas das técnicas utilizadas para gerar animais transgênicos são caras e laboriosas. Neste contexto, a transferência gênica mediada por espermatozóides (TGME) pode ser uma alternativa… (more)

▼

Muitas das técnicas utilizadas para gerar animais transgênicos são caras e laboriosas. Neste contexto, a transferência gênica mediada por espermatozóides (TGME) pode ser uma alternativa para a produção em larga escala de animais transgênicos. Estudos de SMGT têm seu foco no número de cópias de DNA incorporada pelo espermatozóide. Por isso, há pouca informação disponível sobre como as moléculas de DNA se comportam durante o processo de fecundação e quais os efeitos dos protocolos de TGME sobre a célula espermática. Neste sentido, com o objetivo de avaliar a existência de sitio de integração preferencial das moléculas exógenas de DNA no genoma hospedeiro, utilizamos a hibridização in situ para acompanhar a veiculação do transgene durante o processo de fecundação. Foram avaliadas as membranas acrossomais, plasmática e potencial de membrana mitocondrial de espermatozóides submetidos a TGME. Para isso, o sêmen de três diferentes touros foram submetidos ao gradiente de Percoll 45-90%. As células viáveis foram incubadas com o vetor recombinante pCX-EGFP (0, 250, 500 ou 1000ng/106 células) seguidas ou não de eletroporação (300v, 35µF e 0,25ms). Os espermatozóides tratados foram utilizados para a produção in vitro de embriões. Os embriões foram cultivados por sete dias até o estágio de blastocisto. Espermatozóides e embriões produzidos in vitro foram submetidos ao ensaio de hibridização in situ, com metodologia descrita Whyte et al. (2000). O potencial de membrana mitocondrial (PMM), integridade de membrana acrossomal (MA) e plasmática (MP) foram avaliados por citometria de fluxo (Guava Technologies, Hayward, CA, USA) utilizando as sondas fluorescentes JC1, FITC-PSA e PI (Molecular Probes), respectivamente. Os dados foram analisados pelo teste paramétrico ANOVA (teste LSD) usando o programa estatístico SAS for Windows, com nível de significância de 5%. A hibridização in situ não foi possível em espermatozóides bovinos, pois não houve hibridação da sonda controle. Blastocistos oriundos de espermatozóides incubados com DNA exógeno apresentaram integração de forma difusa, embriões oriundos de espermatozóides eletroporados apresentaram integração pontual. As diferentes concentrações de DNA não exerceram efeitos deletérios nas MP ou PMM, a adição de 500ng de DNA causou aumento de lesão na MA (p<0,05). A eletroporação não afeta a MP e MA, mais apresenta grande efeito no PMM causando redução da função mitocondrial. Este estudo conclui que maiores esforços são necessários para elucidar o comportamento das moléculas exógenas de DNA durante o processo de fecundação e quais são os efeitos da TGME sobre a célula espermática.

Most techniques used to produce transgenic animals are laborious and expensive. In this manner, sperm mediated gene transfer (SMGT) may be a viable alternative for long-scale production of transgenic animals. Many SMGT studies have focused the DNA internalization and number of DNA copies incorporated by spermatozoa. However, limited data is available about how foreign DNA…

Advisors/Committee Members: Assumpção, Mayra Elena Ortiz D\'Avila.

Subjects/Keywords: In vitro fertilization; Incubation.; Sperm Mediated Gene Transfer (SMGT). Transgenic Animals. Electroporation; Transferência Gênica Mediada por Espermatozóides (TGME). Animais Transgênicos. Eletroporação. Fecun

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Cavalcanti, P. V. (2010). Hibridização in situ em espermatozóides bovinos tratados com DNA exógeno: estudo experimental. (Masters Thesis). University of São Paulo. Retrieved from http://www.teses.usp.br/teses/disponiveis/10/10131/tde-02022011-211958/ ;

Chicago Manual of Style (16^th Edition):

Cavalcanti, Paulo Varoni. “Hibridização in situ em espermatozóides bovinos tratados com DNA exógeno: estudo experimental.” 2010. Masters Thesis, University of São Paulo. Accessed January 16, 2021. http://www.teses.usp.br/teses/disponiveis/10/10131/tde-02022011-211958/ ;.

MLA Handbook (7^th Edition):

Cavalcanti, Paulo Varoni. “Hibridização in situ em espermatozóides bovinos tratados com DNA exógeno: estudo experimental.” 2010. Web. 16 Jan 2021.

Vancouver:

Cavalcanti PV. Hibridização in situ em espermatozóides bovinos tratados com DNA exógeno: estudo experimental. [Internet] [Masters thesis]. University of São Paulo; 2010. [cited 2021 Jan 16]. Available from: http://www.teses.usp.br/teses/disponiveis/10/10131/tde-02022011-211958/ ;.

Council of Science Editors:

Cavalcanti PV. Hibridização in situ em espermatozóides bovinos tratados com DNA exógeno: estudo experimental. [Masters Thesis]. University of São Paulo; 2010. Available from: http://www.teses.usp.br/teses/disponiveis/10/10131/tde-02022011-211958/ ;

University of Georgia

13. Behringer, Megan Grace. Evolutionary dynamics of genome architecture.

Degree: 2015, University of Georgia

URL: http://hdl.handle.net/10724/32517

► With sequencing technology becoming more advanced and widely available, so has the ability to describe the forces and processes shaping genome architecture. Spontaneous mutations such… (more)

Subjects/Keywords: Mutation; Recombination; Gene Conversion; Nonsense Mediated Decay

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Behringer, M. G. (2015). Evolutionary dynamics of genome architecture. (Thesis). University of Georgia. Retrieved from http://hdl.handle.net/10724/32517

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Behringer, Megan Grace. “Evolutionary dynamics of genome architecture.” 2015. Thesis, University of Georgia. Accessed January 16, 2021. http://hdl.handle.net/10724/32517.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Behringer, Megan Grace. “Evolutionary dynamics of genome architecture.” 2015. Web. 16 Jan 2021.

Vancouver:

Behringer MG. Evolutionary dynamics of genome architecture. [Internet] [Thesis]. University of Georgia; 2015. [cited 2021 Jan 16]. Available from: http://hdl.handle.net/10724/32517.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Behringer MG. Evolutionary dynamics of genome architecture. [Thesis]. University of Georgia; 2015. Available from: http://hdl.handle.net/10724/32517

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Virginia Tech

14. Harb, Amal Mohammad. Dissection of Drought Responses in Arabidopsis.

Degree: PhD, Biology, 2010, Virginia Tech

URL: http://hdl.handle.net/10919/77148

► Plants as sessile organisms are susceptible to many environmental stresses such as drought, and salinity. They have therefore evolved mechanisms to acclimate and tolerate environmental… (more)

▼ Plants as sessile organisms are susceptible to many environmental stresses such as drought, and salinity. They have therefore evolved mechanisms to acclimate and tolerate environmental stresses. Knowledge of the molecular aspects of abiotic stress gleaned from extensive studies in Arabidopsis has provided much information on the complex processes underlying plant response to abiotic stresses. Nevertheless, there is a need for integration of the knowledge gained and a systematic molecular genetic dissection of the complex responses to abiotic stress. In this study in Arabidopsis, comparative expression profiling analysis of progressive (pDr) and moderate (mDr) drought treatments revealed common drought responses, as well as treatment specific signatures responses to drought stress. Under prolonged moderate drought plants develop different mechanisms for acclimation: induction of cell wall loosening at early stage, and a change in hormonal balance (ABA: JA) at late stage of moderate drought. Taking a reverse genetics approach, a MYB transcription factor (MYB109) has been identified as a regulator of growth under drought and salt stress. Global expression profiling showed possible mechanisms of how MYB109 modulates growth under drought conditions: as a regulator of RNA processing and splicing and as a negative regulator of jasmonic acid biosynthesis and signaling. A forward genetics screen for drought and salt tolerance of transposon activation tag (ATag) lines led to the discovery of novel genes, which shed light on unexplored areas of abiotic stress biology. Utilizing this strategy, a potential role for cell wall modification and MATE transporters in response to drought and salt stress has been discovered, which needs further analysis to integrate this information on the role of these biological processes in plant stress biology. Advisors/Committee Members: Grene, Ruth (committee member), Saghai-Maroof, Mohammad A. (committee member), Lawrence, Christopher B. (committee member), Hilu, Khidir W. (committeecochair), Pereira, Andy (committeecochair).

Subjects/Keywords: gene; hormone; cell wall; knockout; transposon

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APA (6^th Edition):

Harb, A. M. (2010). Dissection of Drought Responses in Arabidopsis. (Doctoral Dissertation). Virginia Tech. Retrieved from http://hdl.handle.net/10919/77148

Chicago Manual of Style (16^th Edition):

Harb, Amal Mohammad. “Dissection of Drought Responses in Arabidopsis.” 2010. Doctoral Dissertation, Virginia Tech. Accessed January 16, 2021. http://hdl.handle.net/10919/77148.

MLA Handbook (7^th Edition):

Harb, Amal Mohammad. “Dissection of Drought Responses in Arabidopsis.” 2010. Web. 16 Jan 2021.

Vancouver:

Harb AM. Dissection of Drought Responses in Arabidopsis. [Internet] [Doctoral dissertation]. Virginia Tech; 2010. [cited 2021 Jan 16]. Available from: http://hdl.handle.net/10919/77148.

Council of Science Editors:

Harb AM. Dissection of Drought Responses in Arabidopsis. [Doctoral Dissertation]. Virginia Tech; 2010. Available from: http://hdl.handle.net/10919/77148

Harvard University

15. Hsu, Tiffany Yeong-Ting. Inter-species interactions in microbial communities.

Degree: PhD, 2017, Harvard University

URL: http://nrs.harvard.edu/urn-3:HUL.InstRepos:42015251

►

Microorganisms are omnipresent and exist as communities within and around the human body. These communities, regardless of location, may cause disease: dysbioses within the gut… (more)

▼

Microorganisms are omnipresent and exist as communities within and around the human body. These communities, regardless of location, may cause disease: dysbioses within the gut microbiota are associated with obesity and inflammatory bowel disease, while differences in immune development and environmental exposures are linked to atopy and diabetes. It is thus crucial to characterize microbial communities and their interactions to better understand how they are formed, maintained, and manipulated. To better understand the ecology of communities on and around the human body, my work has explored lateral gene transfer (LGT) within human-associated microbial communities and the transfer of microbes between the human body and environmental surfaces. I developed the first method for detection of de novo LGT events from metagenomes termed WAAFLE, a Workflow to Annotate Assemblies and Find LGT Events. I applied WAAFLE to the Human Microbiome Project: LGT frequencies were highest in the gut and oral sites, and lowest in the vaginal and skin microbiomes. High frequency pairs corresponded with increased taxon abundances and close phylogenetic distances. Taxa found in multiple LGT pairs had strong partner preferences, and several had biases in transfer directionality. Enriched functions in LGT contigs included transposases, phage, and TonB membrane receptors. Taxa in high frequency LGT pairs may preferentially use LGT as a tool to maintain or change their community status. I examined cross-talk between human-associated and built-environment microbial communities in heavily trafficked environments, specifically the Boston subway. These areas may facilitate microbial transmission and are ripe for public health interventions such as sanitation or architecture. We used 16S rRNA gene and metagenomics shotgun sequencing to profile microbes on multiple surface types in trains along the red, green, and orange lines, as well as ticketing machines at four train stations. Community structure was dictated by surface type, rather than train line. Common taxa included human skin and oral commensals such as Propionibacterium, Corynebacterium, Staphylococcus, and Streptococcus. Enriched functions were often from Propionibacterium acnes pathways, and few antibiotic resistance genes were observed. Overall, microbial communities on the Boston subway are likely derived from the rider population and influenced by rider interactions and environmental biochemistry.

Medical Sciences

Advisors/Committee Members: Springer, Michael (advisor), Waldor, Matthew (committee member), Grad, Yonatan (committee member), Polz, Martin (committee member).

Subjects/Keywords: Metagenomics; lateral gene transfer

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Hsu, T. Y. (2017). Inter-species interactions in microbial communities. (Doctoral Dissertation). Harvard University. Retrieved from http://nrs.harvard.edu/urn-3:HUL.InstRepos:42015251

Chicago Manual of Style (16^th Edition):

Hsu, Tiffany Yeong-Ting. “Inter-species interactions in microbial communities.” 2017. Doctoral Dissertation, Harvard University. Accessed January 16, 2021. http://nrs.harvard.edu/urn-3:HUL.InstRepos:42015251.

MLA Handbook (7^th Edition):

Hsu, Tiffany Yeong-Ting. “Inter-species interactions in microbial communities.” 2017. Web. 16 Jan 2021.

Vancouver:

Hsu TY. Inter-species interactions in microbial communities. [Internet] [Doctoral dissertation]. Harvard University; 2017. [cited 2021 Jan 16]. Available from: http://nrs.harvard.edu/urn-3:HUL.InstRepos:42015251.

Council of Science Editors:

Hsu TY. Inter-species interactions in microbial communities. [Doctoral Dissertation]. Harvard University; 2017. Available from: http://nrs.harvard.edu/urn-3:HUL.InstRepos:42015251

Louisiana State University

16. Boudreaux, Charles Mitchell. A novel strategy of controlling bovine pneumonic pasteurellosis: transfecting the upper respiratory tract of cattle with a gene coding for the antimicrobial peptide cecropin B.

Degree: MS, Veterinary Pathology and Pathobiology, 2004, Louisiana State University

URL: etd-07012004-101913 ; https://digitalcommons.lsu.edu/gradschool_theses/645

► The very potent antibacterial activity of cecropin B makes it a likely candidate to prevent and/or treat Mannheimia haemolytica 1:A infection in the upper respiratory… (more)

▼ The very potent antibacterial activity of cecropin B makes it a likely candidate to prevent and/or treat Mannheimia haemolytica 1:A infection in the upper respiratory tract (URT) of cattle. The purpose of this study was to ascertain if the URT could be transfected with a gene coding for the antimicrobial peptide cecropin B. By transfecting cattle with a gene coding for cecropin B, this study attempted to inhibit colonization of a virulent strain of M. haemolytica 1:A in the URT while investigating any possible changes in the indigenous and transient nasal flora. In this study the antibacterial efficacy of cecropin B for a virulent strain of M. haemolytica 1:A was determined. In vitro results showed that cecropin B was very effective in inhibiting this virulent strain of M. haemolytica 1:A within 20 minutes of incubation at 37°C. No inhibition of its activity was observed by incubating cecropin B in pooled bovine nasal secretions. The nasal passages of calves were aerosolized with different amounts of plasmid DNA containing a gene coding for cecropin B. Results of this study show that calves transfected with 50 or 100 μg of plasmid DNA per nostril were able to express cecropin B at the mRNA and peptide level. Detection of the cecropin B gene in control calves may indicate the possibility of native bovine cecropin. After challenge with a virulent strain of M. haemolytica 1:A, all calves were stressed by transportation in a crowded trailer 100 miles for 3 hours. Seven out of the 8 control calves yielded detectible levels of M. haemolytica 1:A in nasal aspirates throughout the weeks following challenge. All 4 calves given 25 μg of plasmid DNA per nostril and two of the 4 calves given 50 μg of plasmid DNA per nostril yielded detectible levels of M. haemolytica 1:A in nasal aspirates following challenge. However, M. haemolytica 1:A was not detected in any calf given 100 μg of plasmid DNA per nostril. There appeared to be no change in the normal bacterial nasal flora.

Subjects/Keywords: gene transfer

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APA (6^th Edition):

Boudreaux, C. M. (2004). A novel strategy of controlling bovine pneumonic pasteurellosis: transfecting the upper respiratory tract of cattle with a gene coding for the antimicrobial peptide cecropin B. (Masters Thesis). Louisiana State University. Retrieved from etd-07012004-101913 ; https://digitalcommons.lsu.edu/gradschool_theses/645

Chicago Manual of Style (16^th Edition):

Boudreaux, Charles Mitchell. “A novel strategy of controlling bovine pneumonic pasteurellosis: transfecting the upper respiratory tract of cattle with a gene coding for the antimicrobial peptide cecropin B.” 2004. Masters Thesis, Louisiana State University. Accessed January 16, 2021. etd-07012004-101913 ; https://digitalcommons.lsu.edu/gradschool_theses/645.

MLA Handbook (7^th Edition):

Boudreaux, Charles Mitchell. “A novel strategy of controlling bovine pneumonic pasteurellosis: transfecting the upper respiratory tract of cattle with a gene coding for the antimicrobial peptide cecropin B.” 2004. Web. 16 Jan 2021.

Vancouver:

Boudreaux CM. A novel strategy of controlling bovine pneumonic pasteurellosis: transfecting the upper respiratory tract of cattle with a gene coding for the antimicrobial peptide cecropin B. [Internet] [Masters thesis]. Louisiana State University; 2004. [cited 2021 Jan 16]. Available from: etd-07012004-101913 ; https://digitalcommons.lsu.edu/gradschool_theses/645.

Council of Science Editors:

Boudreaux CM. A novel strategy of controlling bovine pneumonic pasteurellosis: transfecting the upper respiratory tract of cattle with a gene coding for the antimicrobial peptide cecropin B. [Masters Thesis]. Louisiana State University; 2004. Available from: etd-07012004-101913 ; https://digitalcommons.lsu.edu/gradschool_theses/645

University of New South Wales

17. Song, Weizhi. Towards the detection of horizontal gene transfer in metagenomic datasets.

Degree: Biotechnology & Biomolecular Sciences, 2019, University of New South Wales

URL: http://handle.unsw.edu.au/1959.4/63741 ; https://unsworks.unsw.edu.au/fapi/datastream/unsworks:60824/SOURCE02?view=true

► Horizontal gene transfer (HGT) is thought to be an important driving force for microbial evolution and adaptation, including the development of antibiotics resistance and niche… (more)

▼ Horizontal gene transfer (HGT) is thought to be an important driving force for microbial evolution and adaptation, including the development of antibiotics resistance and niche adaptation. Metagenomics provides an opportunity to study HGT on the level of microbial communities, however, analysis method for this are currently lacking. Here, I developed three bioinformatic pipelines to aid the detection of HGT in metagenomic datasets.Firstly, Binning_refiner was developed to improve the quality of genome bins derived from metagenomic datasets through the combination of different binning programs. The results demonstrated that Binning_refiner can significantly reduce the contamination level of genome bins and increase the total size of contamination-free genome bins. Secondly, HgtSIM was developed to simulate HGT events among microbial community members with user-defined mutation levels. It was developed for testing and benchmarking pipelines for recovering HGTs from complex microbial communities. Thirdly, MetaCHIP was developed to identify HGTs at the community-level through the combination of best-match and phylogenetic approaches. Assessment of its performance on both simulated and real datasets showed that it can effectively predict HGTs with various degrees of genetic divergence from microbial communities. The results also showed that the detection of very recent gene transfers (i.e. those with genetic divergence < 5%) from metagenomic datasets is affected by the read assemble step, as the genomic background of recently transferred genes cannot be recovered with currently available assemblers. And finally, the potential application of long-read sequencing (PacBio) for metagenomics was also explored. A simulated metagenome of pooled genomic DNA from ten marine bacteria with various degrees of genome similarity was sequenced on the PacBio sequencing platform and ten complete high-quality genomes were assembled. The findings and developments made here, including a reference-based read phasing approach for the assembly of highly similar genomes, can be used in the future to design strategies to analyse long-read sequencing data for mixed bacterial communities. Advisors/Committee Members: Thomas, Torsten, Biological, Earth & Environmental Sciences, Faculty of Science, UNSW, Egan, Suhelen, Biological, Earth & Environmental Sciences, Faculty of Science, UNSW.

Subjects/Keywords: Bioinformatics; Horizontal Gene Transfer; Metagenomics

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Song, W. (2019). Towards the detection of horizontal gene transfer in metagenomic datasets. (Doctoral Dissertation). University of New South Wales. Retrieved from http://handle.unsw.edu.au/1959.4/63741 ; https://unsworks.unsw.edu.au/fapi/datastream/unsworks:60824/SOURCE02?view=true

Chicago Manual of Style (16^th Edition):

Song, Weizhi. “Towards the detection of horizontal gene transfer in metagenomic datasets.” 2019. Doctoral Dissertation, University of New South Wales. Accessed January 16, 2021. http://handle.unsw.edu.au/1959.4/63741 ; https://unsworks.unsw.edu.au/fapi/datastream/unsworks:60824/SOURCE02?view=true.

MLA Handbook (7^th Edition):

Song, Weizhi. “Towards the detection of horizontal gene transfer in metagenomic datasets.” 2019. Web. 16 Jan 2021.

Vancouver:

Song W. Towards the detection of horizontal gene transfer in metagenomic datasets. [Internet] [Doctoral dissertation]. University of New South Wales; 2019. [cited 2021 Jan 16]. Available from: http://handle.unsw.edu.au/1959.4/63741 ; https://unsworks.unsw.edu.au/fapi/datastream/unsworks:60824/SOURCE02?view=true.

Council of Science Editors:

Song W. Towards the detection of horizontal gene transfer in metagenomic datasets. [Doctoral Dissertation]. University of New South Wales; 2019. Available from: http://handle.unsw.edu.au/1959.4/63741 ; https://unsworks.unsw.edu.au/fapi/datastream/unsworks:60824/SOURCE02?view=true

University of Melbourne

18. Auyong, Adelene Shu Mei. Pathogenicity genes of Colletotrichum truncatum, causal agent of chili (Capsicum spp.) anthracnose disease.

Degree: 2011, University of Melbourne

URL: http://hdl.handle.net/11343/36747

► Colletotrichum truncatum (Schwein.), Andrus & W.D. Moore (1935) is the main pathogen of chili (Capsicum spp.) causing anthracnose disease in the tropical and sub-tropical regions… (more)

▼ Colletotrichum truncatum (Schwein.), Andrus & W.D. Moore (1935) is the main pathogen of chili (Capsicum spp.) causing anthracnose disease in the tropical and sub-tropical regions of Asia and Australia. Anthracnose disease can cause severe economic loss through reduced yield and marketability of infected fruit. The use of conventional methods to control for anthracnose disease of chili in the field is not effective in reducing crop loss. In severe epidemics, losses of up to 80% of the total yield can be quite significant to the industry. Furthermore, no resistant commercial cultivars of chili (Capsicum annuum) exist, although resistance has been detected in genotypes of the related species, C. chinense and C. baccatum. Knowledge of the molecular genetic mechanism(s) of pathogen infection and the processes that trigger the host defence strategies would be informative towards developing/breeding for resistant chili genotypes. Pathogenesis of C. truncatum on chili is an intricate and complex process which is an outcome of the expression of various pathogenicity-related (PR) genes that evade or overcome host defences. In this study, PR genes of two C. truncatum pathotypes F8-3B and BRIP26974, possibly involved during the infection of Capsicum were isolated using a PCR-targeted approach. Molecular identification revealed that almost all of the targeted PR genes were present in the genome of C. truncatum. EndoPG1 was found to be highly homologous to the PG1 in C. lindemuthianum and Cut1 to the cutinase in C. capsici and C. gloeosporioides. The conventional and modern PCR techniques such as RT-PCR and qRT-PCR were used to qualitatively and quantitatively screen and predict genes that were differentially expressed at the early stage of C. truncatum infection. Expression profiles of Cap20, EndoPG1, G-Protα, CLSP1 and Cut1 were differentially regulated during the saprophytic and pathogenic stages. However, significant expression changes were observed in the cell wall and cuticle degrading enzymes, EndoPG1 and Cut1. Cutinase is an extracellular enzyme involved in the penetration of the host cuticular barrier into the host plant during the initial stages of the fungal infection. Overall, the transcript expression of EndoPG1 reached a maximum at 6 hours after inoculation (HAI). In contrast, Cut1 transcript showed greatest upregulation at 24 HAI. Consequently, the full length CtCut1 gene was isolated from C. truncatum and its molecular characteristics were analysed. To facilitate the study of gene function in fungal-plant interactions between C. truncatum and chili, a robust Agrobacterium tumefaciens-mediated transformation (ATMT) system was developed for C. truncatum. A. tumefaciens carrying a hygromycin phosphotransferase gene (hph) and a green fluorescent protein (gfp) gene was used to transform the conidiospores of C. truncatum pathotypes F8-3B and BRIP26974. Optimum transformation efficiency was obtained when equal amounts of A.…

Subjects/Keywords: Colletotrichum truncatum; chili; anthracnose; pathogenicity genes; Agrobacterium tumefaciens-mediated transformation; RNA-mediated gene silencing

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Auyong, A. S. M. (2011). Pathogenicity genes of Colletotrichum truncatum, causal agent of chili (Capsicum spp.) anthracnose disease. (Doctoral Dissertation). University of Melbourne. Retrieved from http://hdl.handle.net/11343/36747

Chicago Manual of Style (16^th Edition):

Auyong, Adelene Shu Mei. “Pathogenicity genes of Colletotrichum truncatum, causal agent of chili (Capsicum spp.) anthracnose disease.” 2011. Doctoral Dissertation, University of Melbourne. Accessed January 16, 2021. http://hdl.handle.net/11343/36747.

MLA Handbook (7^th Edition):

Auyong, Adelene Shu Mei. “Pathogenicity genes of Colletotrichum truncatum, causal agent of chili (Capsicum spp.) anthracnose disease.” 2011. Web. 16 Jan 2021.

Vancouver:

Auyong ASM. Pathogenicity genes of Colletotrichum truncatum, causal agent of chili (Capsicum spp.) anthracnose disease. [Internet] [Doctoral dissertation]. University of Melbourne; 2011. [cited 2021 Jan 16]. Available from: http://hdl.handle.net/11343/36747.

Council of Science Editors:

Auyong ASM. Pathogenicity genes of Colletotrichum truncatum, causal agent of chili (Capsicum spp.) anthracnose disease. [Doctoral Dissertation]. University of Melbourne; 2011. Available from: http://hdl.handle.net/11343/36747

19. Teixeira, Paula Rezende. Identificação e caracterização de elementos de transposição no genoma de Rhynchosciara.

Degree: PhD, Bioquímica, 2007, University of São Paulo

URL: http://www.teses.usp.br/teses/disponiveis/46/46131/tde-12062008-100547/ ;

► Os elementos de transposição são seqüências discretas que são capazes de mover- se de um lócus para outro, constituindo uma parte significante do genoma de… (more)

▼ Os elementos de transposição são seqüências discretas que são capazes de mover- se de um lócus para outro, constituindo uma parte significante do genoma de eucariotos. São agrupados em duas classes principais, os elementos da Classe I, que se transpõem via um intermediário de RNA (retrotransposon), e os elementos da Classe II, que se transpõem via mecanismo de DNA do tipo corta e cola (transposon). A análise das seqüências de um banco de cDNA construído com RNA mensageiro da glândula salivar de Rhynchosciara americana mostrou a presença de representantes das duas classes de elementos. Nesse trabalho caracteriza-se quarto elementos de transposição tipo mariner, onde as seqüências consenso de nucleotídeos foram derivadas de múltiplas cópias defectivas contendo deleções, mudança no quadro de leitura e códons de terminação. Ramar1, um elemento full-length e Ramar2 um elemento defectivo que contém uma deleção na região interna da ORF da transposase, mas mantém e as extremidades intactas. Ramar3 e Ramar4 são elementos defectivos que apresentam muitas deleções no interior da ORF. As seqüências preditas das transposases demostraram que Ramar1 e Ramar2 estão filogeneticamente muito próximos dos elementos mariner da subfamília mauritiana. Enquanto, Ramar3 e Ramar4 pertencem às subfamílias mellifera e irritans, respectivamente. Hibridização in situ mostrou que Ramar1 localiza-se em muitas regiões do cromossomo, principalmente na heterocromatina pericentromérica, enquanto Ramar2 aparece em uma única banda no cromossomo A. Resultado ainda mais curioso foi a caracterização molecular de um elemento de retrotransposição, denominado RaTART, que provavelmente é o responsável pela reconstituição telomérica em R.americana, assim como os elementos TART, HeT-A e TAHRE de Drosophila. Experimentos de Southern Blots do retroelemento RaTART indicam que este está representado por seqüências repetidas no genoma de R.americana, enquanto que Northern Blots mostraram uma expressão em diferentes estágios do desenvolvimento e o transcrito de alto peso molecular detectado representa o retrotransposon non-LTR inteiro. Enquanto a localização cromossômica de RaTART por hibridização in situ mostrou uma marcação predominante nas extremidades dos cromossomos, indicando possivelmente o primeiro elemento de transposição descrito em R.americana com função definida na estrutura do cromossomo. O último retrotransposon, identificado nesse projeto, presente no genoma de R.americana, denominado R2Ra, foi isolado a partir de uma varredura em um banco genômico construído no bacteriófago lambda dash usando como sonda o recombinante pRa1.4 que contém a unidade de repetição do rDNA. A análise da seqüência mostrou a presença de regiões conservadas, como o domínio de transcriptase reversa e o motivo zinc finger na região amino-terminal. O sítio de inserção no gene 28S do rDNA é altamente conservado em R.americana e a análise filogenética mostrou que este elemento pertence ao grupo R2. A localização cromossômica confirma que o… Advisors/Committee Members: Santelli, Gláucia Maria Machado.

Subjects/Keywords: Rhynchosciara; Rhynchosciara; Diptera; Diptera; Elemento de transposição; Expressão gênica; Gene expression; Genomas; Genome; ITR; ITR; Mariner; Mariner; Retrotransposon; Retrotransposon; Transposable element; Transposon; Transposon

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Teixeira, P. R. (2007). Identificação e caracterização de elementos de transposição no genoma de Rhynchosciara. (Doctoral Dissertation). University of São Paulo. Retrieved from http://www.teses.usp.br/teses/disponiveis/46/46131/tde-12062008-100547/ ;

Chicago Manual of Style (16^th Edition):

Teixeira, Paula Rezende. “Identificação e caracterização de elementos de transposição no genoma de Rhynchosciara.” 2007. Doctoral Dissertation, University of São Paulo. Accessed January 16, 2021. http://www.teses.usp.br/teses/disponiveis/46/46131/tde-12062008-100547/ ;.

MLA Handbook (7^th Edition):

Teixeira, Paula Rezende. “Identificação e caracterização de elementos de transposição no genoma de Rhynchosciara.” 2007. Web. 16 Jan 2021.

Vancouver:

Teixeira PR. Identificação e caracterização de elementos de transposição no genoma de Rhynchosciara. [Internet] [Doctoral dissertation]. University of São Paulo; 2007. [cited 2021 Jan 16]. Available from: http://www.teses.usp.br/teses/disponiveis/46/46131/tde-12062008-100547/ ;.

Council of Science Editors:

Teixeira PR. Identificação e caracterização de elementos de transposição no genoma de Rhynchosciara. [Doctoral Dissertation]. University of São Paulo; 2007. Available from: http://www.teses.usp.br/teses/disponiveis/46/46131/tde-12062008-100547/ ;

University of Melbourne

20. Wan, Yu. Detecting horizontal co-transfer of antimicrobial resistance genes in bacteria: a network approach.

Degree: 2019, University of Melbourne

URL: http://hdl.handle.net/11343/227074

► Antimicrobials have been widely using as a major resource to treat bacterial infections for almost a century. However, it is not unusual to see antimicrobial… (more)

▼ Antimicrobials have been widely using as a major resource to treat bacterial infections for almost a century. However, it is not unusual to see antimicrobial resistance emerges in a bacterial species due to natural selection under the usage of antimicrobials. Moreover, numerous studies show that bacteria can accumulate genes encoding resistance to different classes of antimicrobials and share them with other bacteria regardless of ancestry via a biological process called horizontal gene transfer, causing emergence and fast transmission of multidrug resistance. As such, antimicrobial resistance becomes an urgent and global threat to public health, pushing us backwards to the pre-antimicrobial era. In this thesis, I focus on horizontal co-transfer of resistance genes between bacteria of the same species, which is usually caused by co-localisation of resistance genes in mobile genetic elements, also known as physical linkage between these genes. This kind of linkage plays a pivotal role in the evolution of multidrug resistance, because the mobile elements can translocate, recombine and aggregate, rapidly rendering their host bacteria resistant to a wide spectrum of antimicrobials. By far there is nonetheless not an approach identifying horizontally co-transferred genes in a single bacterial species. Yet most authors of literature reported a few co-mobilised resistance genes each time following biological experiments, and some researchers only applied simple association analysis to representative bacterial isolates of distinct species so as to minimise the possibility that a specific combination of genes is inherited from their most-recent common ancestor. In contrast, intra-species association analysis is severely confounded by strong sample relatedness because of bacterial clonal reproduction. This obstacle leaves a gap between the known high frequency of intra-species horizontal gene transfer and our understandings of this process. This thesis presents a scalable computational approach that uses whole-genome sequencing data to identify co-transferred antimicrobial resistance genes in bacteria collected in a few decades from the same species. Moreover, it demonstrates applications of the approach to three clinically important pathogens and reports key players, patterns and dynamics underlying the horizontal co-transfer of resistance genes within each species. In the first chapter, I provide a background to antimicrobial resistance, horizontal gene transfer, whole-genome sequencing and contemporary bioinformatic techniques. I also summarise outcomes of horizontal gene co-transfer for characteristics that we can utilise for inference of physical linkage. Finally, I compare several statistical approaches determining pairwise association between presence-absence status of genes or alleles in bacteria to justify the necessity of controlling for sample relatedness in association analysis. For the second chapter, I derived a methodology inferring co-transferred genes by integrating gene detection, de novo genome…

Subjects/Keywords: antimicrobial resistance; multidrug resistance; horizontal gene transfer; bacterial pathogen; Escherichia coli; Salmonella enterica; Salmonella Typhimurium; Klebsiella pneumoniae; acquired resistance; computational biology; R package; bacterial genomics; population structure; genetic co-transfer; network analysis; spatiotemporal analysis; dynamic network; association network; network approach; co-occurrence network; genetic co-occurrence; phylogenetic signal; bacterial population; association analysis; computational genomics; population genetics; dissemination of antimicrobial resistance; public health; physical linkage; whole-genome sequencing; mobile genetic element; integron; transposon; plasmid; insertion sequence; mobile gene; lateral gene transfer; phylogeny; phylogenetic reconstruction; phylogenetic signal; linear mixed model; logistic regression; penalised logistic regression; correction for population structure; GeneMates; bioinformatics; DNA sequence; genetic structure

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Wan, Y. (2019). Detecting horizontal co-transfer of antimicrobial resistance genes in bacteria: a network approach. (Doctoral Dissertation). University of Melbourne. Retrieved from http://hdl.handle.net/11343/227074

Chicago Manual of Style (16^th Edition):

Wan, Yu. “Detecting horizontal co-transfer of antimicrobial resistance genes in bacteria: a network approach.” 2019. Doctoral Dissertation, University of Melbourne. Accessed January 16, 2021. http://hdl.handle.net/11343/227074.

MLA Handbook (7^th Edition):

Wan, Yu. “Detecting horizontal co-transfer of antimicrobial resistance genes in bacteria: a network approach.” 2019. Web. 16 Jan 2021.

Vancouver:

Wan Y. Detecting horizontal co-transfer of antimicrobial resistance genes in bacteria: a network approach. [Internet] [Doctoral dissertation]. University of Melbourne; 2019. [cited 2021 Jan 16]. Available from: http://hdl.handle.net/11343/227074.

Council of Science Editors:

Wan Y. Detecting horizontal co-transfer of antimicrobial resistance genes in bacteria: a network approach. [Doctoral Dissertation]. University of Melbourne; 2019. Available from: http://hdl.handle.net/11343/227074

21. Πετροπούλου, Περιστέρα-Ιωάννα. Μελέτη των περιοχών της απολιποπρωτεΐνης Ε που διαμεσολαβούν τη de novo βιοσύνθεση HDL σε πειραματικά μοντέλα ποντικών.

Degree: 2011, University of Patras

URL: http://hdl.handle.net/10889/5014

►

Η HDL είναι ένα μείγμα λιποπρωτεϊνικών σωματιδίων υψηλής πυκνότητας, που ανάλογα με τη σύσταση τους σε λιπίδια μπορούν να είναι δισκοειδή ή σφαιρικά. Η κύρια… (more)

▼

Η HDL είναι ένα μείγμα λιποπρωτεϊνικών σωματιδίων υψηλής πυκνότητας, που ανάλογα με τη σύσταση τους σε λιπίδια μπορούν να είναι δισκοειδή ή σφαιρικά. Η κύρια αθηροπροστατευτική δράση της HDL, οφείλεται στο γεγονός ότι η συγκεκριμένη λιποπρωτεΐνη συλλέγει την περίσσεια χοληστερόλης από τους περιφερικούς ιστούς και τη μεταφέρει στο ήπαρ όπου καταβολίζεται. Επιπλέον, έχει αντιφλεγμονώδη και αντιοξειδωτική δράση. Η κύρια πρωτεΐνη της HDL είναι η απολιποπρωτεΐνη Α-Ι (apoA-I). Ωστόσο, πρόσφατα αποδείχθηκε ότι σε πειραματόζωα με έλλειψη στην apoA-I και κατά συνέπεια στην κλασσική HDL, η απολιποπρωτεΐνη Ε (apoE) αλληλεπιδρά με τον μεταφορέα λιπιδίων ABCA1 προάγοντας την de novo σύνθεση HDL σωματιδίων. Στην παρούσα μελέτη, στόχος ήταν η εύρεση της περιοχής της apoE που είναι υπεύθυνη για την λειτουργική αλληλεπίδραση με τον ABCA1 για το σχηματισμό HDL. Για το σκοπό αυτό, ανασυνδυασμένοι αδενοϊοί που εξέφραζαν καρβοξυ-τελικές συντετμημένες μορφές της apoE4 (AdGFP-E4[1-259], AdGFP-E4[1-229], AdGFP-E4[1-202], AdGFP-E4[1-185]), χορηγήθηκαν σε ποντίκια με έλλειψη στην ApoA-I σε δόση 8x108 pfu και πέντε μέρες μετά τη μόλυνση δείγματα πλάσματος αναλύθηκαν για το σχηματισμό HDL. Κλασματοποίηση των λιποπρωτεϊνών του πλάσματος με υπερφυγοκέντρηση σε διαβάθμιση πυκνότητας καθώς και FPLC χρωματογραφία αποκάλυψε ότι όλες οι συντετμημένες μορφές της apoE4 προάγουν το σχηματισμό HDL. Ανάλυση ηλεκτρονικής μικροσκοπίας με αρνητική χρώση των HDL κλασμάτων, επιβεβαίωσε ότι όλες οι συντετμημένες μορφές της apoE4 είναι ικανές να προάγουν το σχηματισμό σωματιδίων με διάμετρο στην περιοχή της HDL. Τα δεδομένα αυτά οδηγούν στο συμπέρασμα ότι η αμινοτελική περιοχή της apoE που εκτείνεται από τα αμινοξέα 1 έως 185 αρκεί για το σχηματισμό HDL σωματιδίων in vivo. Αυτά τα ευρήματα, ανοίγουν το δρόμο στην έρευνα για το σχεδιασμό βιολογικών φαρμάκων με βάση την apoE για τη θεραπεία της δυσλιπιδαιμίας, της αθηροσκλήρωσης και της στεφανιαίας νόσου.

HDL is a mixture of high density lipoprotein particles that depending on the lipid composition may be discoidal or spherical. The main atheroprotective property of HDL is reverse cholesterol transport, a process that unloads excess cholesterol from peripheral tissues and transports it to the liver for catabolism. HDL has also anti-inflammatory and antioxidant properties. The main protein of HDL is apolipoprotein A-I (apoA-I). However, recently it was shown that in the absence of apoA-I and consequently classical HDL, apolipoprotein E (apoE) interacts functionally with the lipid transporter ABCA1, promoting the de novo synthesis of HDL-like particles. The present study focused on the identification of the domain of apoE that is responsible for the functional interaction with ABCA1 and the formation of apoE-containing HDL. Recombinant attenuated adenoviruses expressing carboxy-terminal truncated forms of apoE4 (apoE4[1-259], apoE4[1-229], apoE4[1-202], and apoE4[1-185]) were administered to apoA-I-deficient mice at a low dose of 8x108 pfu and five days post-infection plasma samples were isolated and analyzed …

Advisors/Committee Members: Κυπραίος, Κυριάκος, Petropoulou, Peristera-Ioanna, Παληογιάννη, Φωτεινή, Παπαχρήστου, Διονύσιος.

Subjects/Keywords: Απολιποπρωτεΐνη Ε; De novo βιοσύνθεση HDL; Ποντίκια με ανεπάρκεια στην ΑpoA-I; Συντετμημένες μορφές της apoE; Γονιδιακή μεταφορά μεσολαβούμενη από αδενοϊούς; 572.684 5; Apolipoprotein E; De novo biogenesis of HDL; ABCA1; ApoA-I deficient mice; Truncated apoE forms; Adenovirus-mediated gene transfer

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Πετροπούλου, �. (2011). Μελέτη των περιοχών της απολιποπρωτεΐνης Ε που διαμεσολαβούν τη de novo βιοσύνθεση HDL σε πειραματικά μοντέλα ποντικών. (Masters Thesis). University of Patras. Retrieved from http://hdl.handle.net/10889/5014

Chicago Manual of Style (16^th Edition):

Πετροπούλου, Περιστέρα-Ιωάννα. “Μελέτη των περιοχών της απολιποπρωτεΐνης Ε που διαμεσολαβούν τη de novo βιοσύνθεση HDL σε πειραματικά μοντέλα ποντικών.” 2011. Masters Thesis, University of Patras. Accessed January 16, 2021. http://hdl.handle.net/10889/5014.

MLA Handbook (7^th Edition):

Πετροπούλου, Περιστέρα-Ιωάννα. “Μελέτη των περιοχών της απολιποπρωτεΐνης Ε που διαμεσολαβούν τη de novo βιοσύνθεση HDL σε πειραματικά μοντέλα ποντικών.” 2011. Web. 16 Jan 2021.

Vancouver:

Πετροπούλου �. Μελέτη των περιοχών της απολιποπρωτεΐνης Ε που διαμεσολαβούν τη de novo βιοσύνθεση HDL σε πειραματικά μοντέλα ποντικών. [Internet] [Masters thesis]. University of Patras; 2011. [cited 2021 Jan 16]. Available from: http://hdl.handle.net/10889/5014.

Council of Science Editors:

Πετροπούλου �. Μελέτη των περιοχών της απολιποπρωτεΐνης Ε που διαμεσολαβούν τη de novo βιοσύνθεση HDL σε πειραματικά μοντέλα ποντικών. [Masters Thesis]. University of Patras; 2011. Available from: http://hdl.handle.net/10889/5014

University of Michigan

22. Rust, Elizabeth McLaurine. Adenovirus-mediated myofilament gene transfer into cardiac myocytes: Effects on myocyte structure and function.

Degree: PhD, Pathology, 1999, University of Michigan

URL: http://hdl.handle.net/2027.42/131774

► The specific aims of this dissertation were twofold: (1) to establish an experimental model system in which cardiac contractile protein expression could be rapidly and… (more)

▼ The specific aims of this dissertation were twofold: (1) to establish an experimental model system in which cardiac contractile protein expression could be rapidly and efficiently altered to study structural and functional relationships, and (2) to assess the structural and functional consequences of ectopic expression of two mutant troponin T proteins which are linked to the disease hypertrophic cardiomyopathy using this experimental model system. In order to accomplish the first goal, two cardiac myocyte culture systems were studied. The first was cardiac myocytes derived from mouse embryonic stem cells differentiating in vitro. Infection of these cultures with recombinant adenovirus vectors led to expression of the transferred gene in ~27% of the cardiac myocytes for up to 21 days without altering cardiac myocyte differentiation or contractile function. The second system studied was adult cardiac myocytes in primary culture. Infection of these cultures with adenovirus vectors led to stable expression of the transferred gene in >90% of the myocytes for the 7 day culture period, without affecting myocyte structure or the ability of the myocytes to respond to calcium activation. Due to the greater efficiency of gene transfer and stable adult cardiac myocyte phenotype structurally and functionally, this second system was used to study the effects of expressing two mutant troponin T proteins on cardiac myocyte structure and function. Expression of each of the mutant troponin T proteins in adult cardiac myocytes led to a significant decrease in the calcium sensitivity of contraction, but did not appear to alter myocyte structure. In conclusion, an experimental model system, adult cardiac myocytes in primary culture, has been defined which can be rapidly and efficiently modified using adenovirus mediated gene transfer. Gene transfer of each of two mutant troponin T proteins resulted in a significantly decreased cardiac contractile response to calcium activation, supporting the role of these two mutant proteins in causing hypertrophic cardiomyopathy. Advisors/Committee Members: Metzger, Joseph M. (advisor), Samuelson, Linda (advisor).

Subjects/Keywords: Adenovirus-mediated; Cardiac Myocytes; Cardiomyopathy; Effects; Function; Gene Transfer; Hypertrophic; Myocyte; Myofilament; Structure; Troponin T

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APA (6^th Edition):

Rust, E. M. (1999). Adenovirus-mediated myofilament gene transfer into cardiac myocytes: Effects on myocyte structure and function. (Doctoral Dissertation). University of Michigan. Retrieved from http://hdl.handle.net/2027.42/131774

Chicago Manual of Style (16^th Edition):

Rust, Elizabeth McLaurine. “Adenovirus-mediated myofilament gene transfer into cardiac myocytes: Effects on myocyte structure and function.” 1999. Doctoral Dissertation, University of Michigan. Accessed January 16, 2021. http://hdl.handle.net/2027.42/131774.

MLA Handbook (7^th Edition):

Rust, Elizabeth McLaurine. “Adenovirus-mediated myofilament gene transfer into cardiac myocytes: Effects on myocyte structure and function.” 1999. Web. 16 Jan 2021.

Vancouver:

Rust EM. Adenovirus-mediated myofilament gene transfer into cardiac myocytes: Effects on myocyte structure and function. [Internet] [Doctoral dissertation]. University of Michigan; 1999. [cited 2021 Jan 16]. Available from: http://hdl.handle.net/2027.42/131774.

Council of Science Editors:

Rust EM. Adenovirus-mediated myofilament gene transfer into cardiac myocytes: Effects on myocyte structure and function. [Doctoral Dissertation]. University of Michigan; 1999. Available from: http://hdl.handle.net/2027.42/131774

Kyoto University / 京都大学

23. Xu, Quan. An active transposon mPing facilitates the discovery of useful flowering time mutant genes in rice : イネにおける活性型転移因子mPingによる迅速な有用開花期突然変異遺伝子の探索.

Degree: 博士(農学), 2014, Kyoto University / 京都大学

URL: http://hdl.handle.net/2433/188761 ; http://dx.doi.org/10.14989/doctor.k18323

新制・課程博士

甲第18323号

農博第2048号

Subjects/Keywords: Rice; Transposon; Flowering time; Mutant gene; mPing

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APA (6^th Edition):

Xu, Q. (2014). An active transposon mPing facilitates the discovery of useful flowering time mutant genes in rice : イネにおける活性型転移因子mPingによる迅速な有用開花期突然変異遺伝子の探索. (Thesis). Kyoto University / 京都大学. Retrieved from http://hdl.handle.net/2433/188761 ; http://dx.doi.org/10.14989/doctor.k18323

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Xu, Quan. “An active transposon mPing facilitates the discovery of useful flowering time mutant genes in rice : イネにおける活性型転移因子mPingによる迅速な有用開花期突然変異遺伝子の探索.” 2014. Thesis, Kyoto University / 京都大学. Accessed January 16, 2021. http://hdl.handle.net/2433/188761 ; http://dx.doi.org/10.14989/doctor.k18323.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Xu, Quan. “An active transposon mPing facilitates the discovery of useful flowering time mutant genes in rice : イネにおける活性型転移因子mPingによる迅速な有用開花期突然変異遺伝子の探索.” 2014. Web. 16 Jan 2021.

Vancouver:

Xu Q. An active transposon mPing facilitates the discovery of useful flowering time mutant genes in rice : イネにおける活性型転移因子mPingによる迅速な有用開花期突然変異遺伝子の探索. [Internet] [Thesis]. Kyoto University / 京都大学; 2014. [cited 2021 Jan 16]. Available from: http://hdl.handle.net/2433/188761 ; http://dx.doi.org/10.14989/doctor.k18323.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Xu Q. An active transposon mPing facilitates the discovery of useful flowering time mutant genes in rice : イネにおける活性型転移因子mPingによる迅速な有用開花期突然変異遺伝子の探索. [Thesis]. Kyoto University / 京都大学; 2014. Available from: http://hdl.handle.net/2433/188761 ; http://dx.doi.org/10.14989/doctor.k18323

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

24. Xu, Quan. An active transposon mPing facilitates the discovery of useful flowering time mutant genes in rice .

Degree: 2014, Kyoto University

URL: http://hdl.handle.net/2433/188761

Subjects/Keywords: Rice; Transposon; Flowering time; Mutant gene; mPing

…between GB and HS254 (b). Mapping of the Se15 gene (c). 7 Figure 2 Copy… …transposon display by using TTG selective base in GB and HS lines (b). Table 1 Effects…

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APA (6^th Edition):

Xu, Q. (2014). An active transposon mPing facilitates the discovery of useful flowering time mutant genes in rice . (Thesis). Kyoto University. Retrieved from http://hdl.handle.net/2433/188761

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Xu, Quan. “An active transposon mPing facilitates the discovery of useful flowering time mutant genes in rice .” 2014. Thesis, Kyoto University. Accessed January 16, 2021. http://hdl.handle.net/2433/188761.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Xu, Quan. “An active transposon mPing facilitates the discovery of useful flowering time mutant genes in rice .” 2014. Web. 16 Jan 2021.

Vancouver:

Xu Q. An active transposon mPing facilitates the discovery of useful flowering time mutant genes in rice . [Internet] [Thesis]. Kyoto University; 2014. [cited 2021 Jan 16]. Available from: http://hdl.handle.net/2433/188761.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Xu Q. An active transposon mPing facilitates the discovery of useful flowering time mutant genes in rice . [Thesis]. Kyoto University; 2014. Available from: http://hdl.handle.net/2433/188761

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

25. Garzon Sanabria, Andrea Juliana. Enhanced Hydrogen Production in Escherichia coli Through Chemical Mutagenesis, Gene Deletion, and Transposon Mutagenesis.

Degree: MS, Chemical Engineering, 2011, Texas A&M University

URL: http://hdl.handle.net/1969.1/ETD-TAMU-2010-05-7799

► We demonstrate that hydrogen production can be increased by random mutagenesis using N-methyl-N'-nitro-N-nitrosoguanidine (MNNG) and that hydrogen production can be further increased in the chemically-mutagenized… (more)

▼ We demonstrate that hydrogen production can be increased by random mutagenesis using N-methyl-N'-nitro-N-nitrosoguanidine (MNNG) and that hydrogen production can be further increased in the chemically-mutagenized strain by targeted gene deletion and overexpression of genes related to formate metabolism. Chemical mutagenesis of Escherichia coli BW25113 hyaB hybC hycE::kan/pBS(Kan)-HycE to form strain 3/86 resulted in 109 +/- 0.5- fold more hydrogen; 3/86 lacks functional hydrogen uptake hydrogenases 1 and 2, has hydrogenproducing hydrogenase 3 inactivated from the chromosome, and has constitutively active hydrogenase 3 based on expression of the large subunit of hydrogenase 3 from a high copy number plasmid. Deleting fdoG, which encodes formate dehydrogenase O, (that diverts formate from hydrogen), from chemical mutagen 3/86 increased hydrogen production 188 +/- 0.50-fold (relative to the unmutagenized strain), and deletion of hycA, which encodes the repressor of formate hydrogen lyase (FHL), increased hydrogen production 232 +/- 0.50-fold. Deleting both fdoG and hycA increased hydrogen production 257 +/- 0.50-fold, and overexpressing fhlA along with the fdoG hycA mutations increased hydrogen 308 +/- 0.52-fold. Whole-transcriptome analysis of chemical mutagen 3/86 revealed 89 genes were induced and 31 genes were repressed. In an effort to identify chromosomal mutations in chemical mutagen 3/86, we performed comparative genome sequencing and identified two chromosomal loci with mutations in coding regions of ftnA and yebJ; however, neither gene was related to the increased hydrogen production as determined by the close vial (short) hydrogen assay. In addition, transposon mutagenesis, which is one of the most efficient strategies for creating random mutations in the genomic DNA, was performed in two different strains: E. coli BW25113 hyaB hybC hycA fdoG::kan/pCA24N-FhlA and E. coli MG1655 to identify beneficial mutations for hydrogen production. As a result of screening 461 E. coli BW25113 hyaB hybC hycA fdoG::kan/pCA24N-FhlA transformants and 1000 E. coli MG1655 transformants, three interesting mutations have been discovered in E. coli BW25113 hyaB hybC hycA fdoG::kan/pCA24N-FhlA transformants (gpsA, dipZ, glgP) and 1 beneficial mutation in E. coli MG1655 transformants (malT). When any of these genes gpsA, dipZ, or glgP is disrupted by Tn5 insertion, hydrogen production decreases 17, 3 and 8-fold, respectively. Additionally, when malT gene is disrupted by Tn5 insertion, hydrogen increases 3.4-fold. Advisors/Committee Members: Wood, Thomas K. (advisor), Jayaraman, Arul (committee member), Chu, Kung H. (committee member).

Subjects/Keywords: Chemical mutagenesis; gene deletion; Hydrogen; transposon mutagenesis

…gene deletions and transposon mutagenesis; (ii) to identify novel regulators of… …Transposon mutagenesis… …15 Table 3-2. Primers used for verifying gene knockout with P1 transduction… …bacterial strains and plasmids used throughout the development of transposon mutagenesis study… …18 Figure 3-3. Gene deletion through P1 transduction…

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APA (6^th Edition):

Garzon Sanabria, A. J. (2011). Enhanced Hydrogen Production in Escherichia coli Through Chemical Mutagenesis, Gene Deletion, and Transposon Mutagenesis. (Masters Thesis). Texas A&M University. Retrieved from http://hdl.handle.net/1969.1/ETD-TAMU-2010-05-7799

Chicago Manual of Style (16^th Edition):

Garzon Sanabria, Andrea Juliana. “Enhanced Hydrogen Production in Escherichia coli Through Chemical Mutagenesis, Gene Deletion, and Transposon Mutagenesis.” 2011. Masters Thesis, Texas A&M University. Accessed January 16, 2021. http://hdl.handle.net/1969.1/ETD-TAMU-2010-05-7799.

MLA Handbook (7^th Edition):

Garzon Sanabria, Andrea Juliana. “Enhanced Hydrogen Production in Escherichia coli Through Chemical Mutagenesis, Gene Deletion, and Transposon Mutagenesis.” 2011. Web. 16 Jan 2021.

Vancouver:

Garzon Sanabria AJ. Enhanced Hydrogen Production in Escherichia coli Through Chemical Mutagenesis, Gene Deletion, and Transposon Mutagenesis. [Internet] [Masters thesis]. Texas A&M University; 2011. [cited 2021 Jan 16]. Available from: http://hdl.handle.net/1969.1/ETD-TAMU-2010-05-7799.

Council of Science Editors:

Garzon Sanabria AJ. Enhanced Hydrogen Production in Escherichia coli Through Chemical Mutagenesis, Gene Deletion, and Transposon Mutagenesis. [Masters Thesis]. Texas A&M University; 2011. Available from: http://hdl.handle.net/1969.1/ETD-TAMU-2010-05-7799

Freie Universität Berlin

26. Escobar Fernández, Helena. Entwicklung eines nicht-viralen zellbasierten Ansatz zur Gentherapie von Dysferlinopathien durch Nutzung des Sleeping Beauty Transposons.

Degree: 2015, Freie Universität Berlin

URL: https://refubium.fu-berlin.de/handle/fub188/13597

► Miyoshi Myopathie (MM), Gliedergürteldystrophie Typ 2B (engl. limb girdle muscular dystrophy 2B, LGMD2B) und die DACM (distal anterior compartment myopathy) sind seltene, autosomal rezessiv vererbte… (more)

▼ Miyoshi Myopathie (MM), Gliedergürteldystrophie Typ 2B (engl. limb girdle muscular dystrophy 2B, LGMD2B) und die DACM (distal anterior compartment myopathy) sind seltene, autosomal rezessiv vererbte Krankheiten, die durch Mutationen im Dysferlin-Gen verursacht werden. Diese muskulären Erkrankungen werden unter dem Begriff Dysferlinopathie zusammengefasst und betreffen die proximale und/oder die distale Muskulatur der Extremitäten. Durch eine zunehmende Schwächung und Atrophie der Muskeln sind Patienten normalerweise bereits 15 Jahre nach Krankheitsbeginn an den Rollstuhl gebunden. Behandlungsmöglichkeiten gibt es hier keine. Das Dysferlin-Gen umfasst einen 150 kb-Bereich der genomischen DNA auf Chromosom 2p13 und beinhaltet 55 Exons mit einer kodierenden Sequenz von 6,2 kb. Dysferlin ist ein 237 kDa Protein der Ferlinfamilie. Es ist in vielen Geweben vorhanden, vor allem im Muskelgewebe, wo es in ausgereiften Muskelfasern, aber auch in Satellitenzellen und Myoblasten exprimiert wird. Dysferlin spielt eine wichtige Rolle bei der Membranreparatur und der Aufrechterhaltung der T-Tubuli Struktur in den Muskelfasern. Auβer dem erhöht sich durch ein defektes Dysferlinprotein die Entzündungsanfälligkeit der Muskelfasern. Die Regenerationsfähigkeit von Muskeln beruht auf den Muskelstammzellen (Satellitenzellen) und ihren Abkömmlingen, den Muskelvorläuferzellen (Myoblasten). Bei Muskelverletzungen, z.B. durch schwere Verwundungen oder durch Muskelerkrankungen, vermehren sich diese Zellen extensiv um das geschädigte Gewebe zu reparieren. Satellitenzellen sind durch die Expression des Transkriptionsfaktors Pax7 gekennzeichnet. Ihre Isolation basiert auf spezifischen Oberflächenmarkern. Zudem haben sie ein hohes Anwachspotential, wenn sie in sich regenerierendes Muskelgewebe tranplantiert werden. Deshalb gilt die autologe oder allogene Transplantation von Satellitenzellen oder Myoblasten als vielversprechende therapeutische Alternative. Da Dysferlinopathien durch ein einzelnes Gen verursacht werden, ist zudem die Gentherapie hier sehr erfolgversprechend. Allerdings werden Gentransferversuche durch die Gröβe der kodierenden Dysferlinsequenz erschwert, da die meisten viralen Vektoren, welche für Gentherapiezwecke benutzt werden, eine zu geringe Ladungskapazität besitzen. Das Sleeping Beauty Transposon ist ein nicht-virales 2-Komponenten Vektorsystem, welches aus der DNA Sequenz des Transposons und einem Transposase Protein besteht. Die Transposase schneidet das Transposon aus dem Donorplasmid heraus und integriert es in das Zielgenom. Da das Transposon so verändert werden kann, dass es jede Zielsequenz beinhalten kann, ist Sleeping Beauty ein sehr wichtiges, nicht-virales, genetisches Werkzeug, das einen stabilen Gentransfer ermöglicht und deshalb häufig in der Gentherapie verwendet wird. Durch das Sleeping Beauty System kann ein bis zu 8 kb groβes Transgen effizient integriert werden. Damit ist es auch für die Integration des vollständigen Dysferlin-Gens sehr gut geeignet. Im Rahmen dieser Arbeit habe ich eine Methode entwickelt,… Advisors/Committee Members: [email protected] (contact), w (gender), Prof. Dr. med. Simone Spuler (firstReferee), Prof. Dr. Sigmar Stricker (furtherReferee).

Subjects/Keywords: dysferlin; gene therapy; Sleeping Beauty transposon; myoblast transplantation; 500 Naturwissenschaften und Mathematik::570 Biowissenschaften; Biologie::572 Biochemie

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Escobar Fernández, H. (2015). Entwicklung eines nicht-viralen zellbasierten Ansatz zur Gentherapie von Dysferlinopathien durch Nutzung des Sleeping Beauty Transposons. (Thesis). Freie Universität Berlin. Retrieved from https://refubium.fu-berlin.de/handle/fub188/13597

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Escobar Fernández, Helena. “Entwicklung eines nicht-viralen zellbasierten Ansatz zur Gentherapie von Dysferlinopathien durch Nutzung des Sleeping Beauty Transposons.” 2015. Thesis, Freie Universität Berlin. Accessed January 16, 2021. https://refubium.fu-berlin.de/handle/fub188/13597.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Escobar Fernández, Helena. “Entwicklung eines nicht-viralen zellbasierten Ansatz zur Gentherapie von Dysferlinopathien durch Nutzung des Sleeping Beauty Transposons.” 2015. Web. 16 Jan 2021.

Vancouver:

Escobar Fernández H. Entwicklung eines nicht-viralen zellbasierten Ansatz zur Gentherapie von Dysferlinopathien durch Nutzung des Sleeping Beauty Transposons. [Internet] [Thesis]. Freie Universität Berlin; 2015. [cited 2021 Jan 16]. Available from: https://refubium.fu-berlin.de/handle/fub188/13597.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Escobar Fernández H. Entwicklung eines nicht-viralen zellbasierten Ansatz zur Gentherapie von Dysferlinopathien durch Nutzung des Sleeping Beauty Transposons. [Thesis]. Freie Universität Berlin; 2015. Available from: https://refubium.fu-berlin.de/handle/fub188/13597

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

27. Westcot, Stephanie. Revealing Novel Skin Biology Using Protein-Trap Gene-Break Transposon Mutagenesis Technology In The Larval Zebrafish Model.

Degree: PhD, Molecular, Cellular, Developmental Biology and Genetics, 2016, University of Minnesota

URL: http://hdl.handle.net/11299/185197

► Abstract Although skin disorders affect as much as a third of the population at any given time, available treatments are limited. Because a more comprehensive… (more)

▼ Abstract Although skin disorders affect as much as a third of the population at any given time, available treatments are limited. Because a more comprehensive understanding of skin development mechanisms can spur the identification of new treatment targets and techniques, we developed the Zebrafish Integument Project (ZIP), an expression-driven platform for identifying new skin genes and new, revertible phenotypes in the vertebrate model Danio rerio (zebrafish). In vivo selection for skin-specific expression of gene-break transposon (GBT) mutant lines identified eleven new, revertible GBT alleles of genes involved in skin development. Eight of those genes had been described in an integumentary context to varying degrees: fras1, grip1, hmcn1, msxc, col4a4, ahnak, capn12, and nrg2a. Three others—arhgef25b, fkbp10b, and megf6a—emerged as novel skin genes. Embryos homozygous for a GBT insertion in neuregulin 2a (nrg2a) revealed a novel requirement for a Neuregulin 2a (Nrg2a) – ErbB2/3 – AKT signaling pathway governing ridge cell morphogenesis and apicobasal organization during median fin fold (MFF) morphogenesis. In nrg2a mutant larvae, the basal keratinocytes that comprise the apical MFF (ridge cells) displayed reduced pAKT levels as well as reduced apical domains and exaggerated basolateral domains. Those defects prevented proper ridge cell elongation into a flattened epithelial morphology, resulting in thickened MFF edges. Additionally, morpholino knockdown of epithelial polarity regulator and tumor suppressor lgl2 ameliorated the nrg2a mutant phenotype. Identifying Lgl2 as an antagonist of Nrg2a – ErbB signaling revealed a significantly earlier role for Lgl2 during epidermal morphogenesis than has been described to date. Furthermore, our findings demonstrated that ridge cells’ squamous flattening morphogenesis drives apical MFF development. We therefore propose MFF ridge cells as a new model for investigating the regulation of cell polarity and cellular morphogenesis with regard to their roles as crucial mechanisms for epithelial morphogenesis generally, and for flattening morphogenesis in particular.

Subjects/Keywords: gene-break transposon; neuregulin 2; skin; zebrafish

…4. Gene–break transposon–based protein trapping identifies known and new epidermal median… …internal reinforcement. 14 Section 3: Gene-break Transposon Mutagenesis and Vertebrate… …cause epigenetic silencing (142, 143). Gene-break transposon insertional mutagenesis… …Our laboratory developed a novel insertional mutagen, the protein-trap gene-break transposon… …A) A schematic of the RP2.1 gene-break transposon (GBT) vector used in this…

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APA (6^th Edition):

Westcot, S. (2016). Revealing Novel Skin Biology Using Protein-Trap Gene-Break Transposon Mutagenesis Technology In The Larval Zebrafish Model. (Doctoral Dissertation). University of Minnesota. Retrieved from http://hdl.handle.net/11299/185197

Chicago Manual of Style (16^th Edition):

Westcot, Stephanie. “Revealing Novel Skin Biology Using Protein-Trap Gene-Break Transposon Mutagenesis Technology In The Larval Zebrafish Model.” 2016. Doctoral Dissertation, University of Minnesota. Accessed January 16, 2021. http://hdl.handle.net/11299/185197.

MLA Handbook (7^th Edition):

Westcot, Stephanie. “Revealing Novel Skin Biology Using Protein-Trap Gene-Break Transposon Mutagenesis Technology In The Larval Zebrafish Model.” 2016. Web. 16 Jan 2021.

Vancouver:

Westcot S. Revealing Novel Skin Biology Using Protein-Trap Gene-Break Transposon Mutagenesis Technology In The Larval Zebrafish Model. [Internet] [Doctoral dissertation]. University of Minnesota; 2016. [cited 2021 Jan 16]. Available from: http://hdl.handle.net/11299/185197.

Council of Science Editors:

Westcot S. Revealing Novel Skin Biology Using Protein-Trap Gene-Break Transposon Mutagenesis Technology In The Larval Zebrafish Model. [Doctoral Dissertation]. University of Minnesota; 2016. Available from: http://hdl.handle.net/11299/185197

28. Chatterjee, Payel. Environmental Pseudomonas are a source of Novel Antibiotics that inhibit Cystic fibrosis derived pathogenic Pseudomonas aeruginosa.

Degree: PhD, Biological Sciences, 2017, Bowling Green State University

URL: http://rave.ohiolink.edu/etdc/view?acc_num=bgsu1510071430516639

► The emergence of antimicrobial resistance bacteria has become a major threat to human society. The rapid spread of resistant pathogens and the associated loss of… (more)

▼ The emergence of antimicrobial resistance bacteria has become a major threat to human society. The rapid spread of resistant pathogens and the associated loss of efficacy of available drugs needs to be met with the development of antibiotics and alternative treatments. Pseudomonas aeruginosa is an opportunistic human pathogen evolving resistance to many currently used antibiotics. Chronic lung infections with the bacterium P. aeruginosa are the leading cause of morbidity and mortality in cystic fibrosis (CF) patients. Escalating this problem is that pharmaceutical companies have dropped drug development due to low profitability, thus making the efforts of drug discovery of prime importance. To address this global health threat research institutes have now stepped forward to aid in discovery of novel compounds. P. aeruginosa dominates the lungs during chronic infections in CF patients, yet it’s abundance in non-human habitats such as water and soil is less compared to other diverse groups of pseudomonads. A trait that could contribute to such decreased abundance is bacterial competition from other Pseudomonas populations that dominate water and soil habitats. We hypothesized that environmental Pseudomonas from diverse soil and water habitats produce secondary metabolites capable of inhibiting the growth of CF derived P. aeruginosa. Here, we sought to determine if clinical isolates of P. aeruginosa are susceptible to competition by environmental pseudomonads which may provide a source of inhibitory factors. We have used a population based study in association with transposon mutagenesis, PCR techniques, whole genome sequencing and bioinformatic analysis to identify environmental Pseudomonas biosynthetic gene clusters (BGCs) and characterize antagonistic compounds that are effective against CF-derived P. aeruginosa. A total of five BGCs have been identified in this study from environmental Pseudomonas strains S4B6, S3F9 (soil-derived) and LE6C6 (water-derived) encoding diverse compounds such as bacteriocins, NRPSs, phenazines, and siderophores involved in antagonistic activity. Extending this analysis, we have also identified environmental Pseudomonas that inhibit not only CF-derived P. aeruginosa but are effective against other pathogens including ESKAPE pathogens and carbapenem resistant P. aeruginosa. Overall, this research serves as a platform for the identification of novel antibiotics from these environmental isolates. Advisors/Committee Members: Wildschutte, Hans (Advisor).

Subjects/Keywords: Microbiology; Biology; Cystic fibrosis; antibiotic; multi-drug resistance; antibiotic resistance; Pseudomonas aeruginosa; biosynthetic gene cluster; antagonistic; mutant; transposon

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APA (6^th Edition):

Chatterjee, P. (2017). Environmental Pseudomonas are a source of Novel Antibiotics that inhibit Cystic fibrosis derived pathogenic Pseudomonas aeruginosa. (Doctoral Dissertation). Bowling Green State University. Retrieved from http://rave.ohiolink.edu/etdc/view?acc_num=bgsu1510071430516639

Chicago Manual of Style (16^th Edition):

Chatterjee, Payel. “Environmental Pseudomonas are a source of Novel Antibiotics that inhibit Cystic fibrosis derived pathogenic Pseudomonas aeruginosa.” 2017. Doctoral Dissertation, Bowling Green State University. Accessed January 16, 2021. http://rave.ohiolink.edu/etdc/view?acc_num=bgsu1510071430516639.

MLA Handbook (7^th Edition):

Chatterjee, Payel. “Environmental Pseudomonas are a source of Novel Antibiotics that inhibit Cystic fibrosis derived pathogenic Pseudomonas aeruginosa.” 2017. Web. 16 Jan 2021.

Vancouver:

Chatterjee P. Environmental Pseudomonas are a source of Novel Antibiotics that inhibit Cystic fibrosis derived pathogenic Pseudomonas aeruginosa. [Internet] [Doctoral dissertation]. Bowling Green State University; 2017. [cited 2021 Jan 16]. Available from: http://rave.ohiolink.edu/etdc/view?acc_num=bgsu1510071430516639.

Council of Science Editors:

Chatterjee P. Environmental Pseudomonas are a source of Novel Antibiotics that inhibit Cystic fibrosis derived pathogenic Pseudomonas aeruginosa. [Doctoral Dissertation]. Bowling Green State University; 2017. Available from: http://rave.ohiolink.edu/etdc/view?acc_num=bgsu1510071430516639

29. Lotti, Samantha Nicole. Using CRISPR/Cas9 to modify the genome of cattle.

Degree: MS, Animal Sciences, 2017, University of Illinois – Urbana-Champaign

URL: http://hdl.handle.net/2142/97258

► Genetically modifying animals is a tool that can be used to increase livestock production. The gene editing technology CRISPR has expanded the possibilities of gene… (more)

▼ Genetically modifying animals is a tool that can be used to increase livestock production. The gene editing technology CRISPR has expanded the possibilities of gene editing. The Cas9 nuclease creates a double strand break which can be repaired by non-homologous end joining (NHEJ) or homology directed repair (HDR). The goal of this experiment was to create a single nucleotide change using the CRISPR/Cas9 system in combination with a single strand oligo nucleotide (ssODN). The single nucleotide that was targeted occurred naturally in Holstein cattle, and is associated with increase milk production. There are multiple factors involved in the Holstein cow's ability to produce large amounts of milk. A mutation in the alpha lactalbumin sequence (α-LA) is one of these factors. The α-LA gene sequence in some Holstein cattle contain an adenine the at (+15) position which corresponds to transcriptional start point of α-LA (+1), while other breeds have a guanine at that position. The adenine in the +15 position of the α-LA gene has been associated with increased milk production. MAC-T cells and Angus fetal fibroblasts were transfected with a Cas9 plasmid, pSpCas9(BB)-2A-GFP, and a ssODN to insert the desired mutation. Using the CRISPR/Cas9 system we were able to create a double strand break resulting in indels and deletions at the (+15) site in MAC-T cells, but we were not successful in creating a single nucleotide change. However, we did see a single nucleotide change in Angus fetal fibroblasts using CRISPRs and ssODN. Following the success of inserting the mutation into a cell line we attempted to create an embryo containing the single nucleotide change using sperm-mediated gene transfer (SMGT). Naked DNA binds naturally to sperm, and can be used to produce transgenic offspring in pigs and cattle. In this experiment, we analyzed methods to select thawed bovine sperm, and evaluated the binding of exogenous DNA to those sperm. Liposome preparation was done using a cationic lipid, 3-(trimethyl ammonium iodide) 1,2 dimystryl-propanediate (TAID) and a neutral lipid, L- Dioleoyl phosphatidyl-ethanolamine (DOPE) prepared according to given protocol. Percoll or swim-up methods were used to select sperm after thawing, followed by incubation (1h or 3h) with the liposome-DNA complexes. We used enhanced green fluorescent protein (eGFP) in combination with the liposomes as a marker for exogenous DNA binding. Five treatments per selection method were analyzed: 1) no incubation, no liposomes and no DNA, 2) incubation with no liposomes and no DNA, 3) incubation with liposomes and no DNA, 4) incubation with liposomes and 1 ng of DNA and 5) incubation with liposomes and 10 ng of DNA. Once the liposomes had been complexed with DNA they were incubated with sperm for one or three hours before IVF. The CASA results for total motility and rapid motility were significantly different from the control (P<0.01) between the control and the other treatments in the Percoll group as opposed to swim-up. These results confirm that the sperm selected with… Advisors/Committee Members: Wheeler, Matthew B (advisor), Hurley, Walter (committee member), Beever, Jonathan (committee member).

Subjects/Keywords: Clustered regularly interspaced short palindromic repeats (CRISPR); Sperm-mediated gene transfer (SMGT); Gene editing; Gene transfer; Alpha-lactalbumin; MAC-T cells; Angus fetal fibroblasts

…made using sperm-mediated gene transfer in rabbits with viral DNA (44). Although it… …methods such as sperm-mediated gene transfer (SMGT) or somatic cell nuclear transfer… …delivery methods. Sperm-mediated gene transfer (SMGT) is also commonly used, and can… …development. 1996;8:1055-60. Perez A, Solano R, Castro F, et al. Sperm cells mediated gene transfer… …K, Spadafora C. Sperm-mediated gene transfer: Applications and implications. Bioessays…

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APA (6^th Edition):

Lotti, S. N. (2017). Using CRISPR/Cas9 to modify the genome of cattle. (Thesis). University of Illinois – Urbana-Champaign. Retrieved from http://hdl.handle.net/2142/97258

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Lotti, Samantha Nicole. “Using CRISPR/Cas9 to modify the genome of cattle.” 2017. Thesis, University of Illinois – Urbana-Champaign. Accessed January 16, 2021. http://hdl.handle.net/2142/97258.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Lotti, Samantha Nicole. “Using CRISPR/Cas9 to modify the genome of cattle.” 2017. Web. 16 Jan 2021.

Vancouver:

Lotti SN. Using CRISPR/Cas9 to modify the genome of cattle. [Internet] [Thesis]. University of Illinois – Urbana-Champaign; 2017. [cited 2021 Jan 16]. Available from: http://hdl.handle.net/2142/97258.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Lotti SN. Using CRISPR/Cas9 to modify the genome of cattle. [Thesis]. University of Illinois – Urbana-Champaign; 2017. Available from: http://hdl.handle.net/2142/97258

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Southern California

30. Sumiyoshi, Teiko. Non-viral and viral hematopoietic progenitor cell gene therapy.

Degree: PhD, Molecular Microbiology & Immunology, 2009, University of Southern California

URL: http://digitallibrary.usc.edu/cdm/compoundobject/collection/p15799coll127/id/182101/rec/4447

► The pluripotent characteristic of hematopoietic stem cells (HSCs) makes them a good candidate for gene therapy. The safety drawbacks of the commonly used viral gene… (more)

▼ The pluripotent characteristic of hematopoietic stem cells (HSCs) makes them a good candidate for gene therapy. The safety drawbacks of the commonly used viral gene transfer system have made the search for alternative gene transfer methods such as non-viral or hybrid gene transfer systems became increasingly appealing in the field. One such system is the Sleeping Beauty (SB) transposon-mediated gene transfer system. Using a non-viral approach to delivery SB plasmids we were able to significantly increase the efficiency of stable gene up to 20-fold higher than previously published data by incrementally optimizing each element of the SB transposon system. In vivo studies demonstrated that SB-modified human CD34+ cells were engrafted in NOD/SCID/yC(null) (NSG) mice and differentiated into multi-lineage cell types with stable transgene expression. Transgene expression remained persistent in the secondary transplanted NSG mice indicating a long-term stable integration achieved by HSB-transposon system. Non-integrating lentiviral (NIL) vectors were also investigated as another method for SB plasmid delivery. Combining the stable integration of the SB transposon system with the delivery efficiency of NIL, termed NILting beauty, could produce a hybrid vector system that synergizes the advantages of both viral and non-viral vector systems and provide a more effective and safer approach to genetically modify HSCs. The feasibility and potential of utilizing NILting beauty to achieve stable transgene integration was evaluated using K562 and human HSCs. Up to 7% stable transgene expression was achieved in K562 cells and around 1% for human CD34+ cells when transduced with NILting beauty vectors. The other approach to increase long-term transgene expression with relatively minimal adverse effects in clinical HSC gene therapy is using non-myeloablative conditioning regimen.; The feasibility of combining busulfan with fludarabine as an alternative and potentially more effective conditioning regimen was explored to achieve long-term stable gene marking in HSC gene therapy. We hypothesized that the addition of the immunosuppressive chemotherapeutic agent fludarabine may contribute to better HSC engraftment and long term transgene expression by reducing host immunological responses to the foreign transgene product. To evaluate this hypothesis, a clinically relevant infant rhesus monkey bone marrow transplant (BMT) model was used. Preliminary data showed a strong correlation between the busulfan dose and the busulfan area-under-the curve (AUC). Transient neutropenia was noted whereas lymphopenia was not observed. While monkeys with high levels of eGFP gene marking also showed detectable levels of anti-eGFP antibodies when no fludarabine was given, they lacked humoral immune responses to eGFP if they received fludarabine. These data suggest that the immune responses against the transgene may play a significant role in the successful outcome of HSC gene therapy and that fludarabine may be able to modulate these responses. Since… Advisors/Committee Members: McMillan, Minnie (Committee Chair), Ou, Jing-Hsiung James (Committee Member), Cannon, Paula M. (Committee Member), Kohn, Donald B. (Committee Member).

Subjects/Keywords: pluripotent; hematopoietic stem cells (HSCs); gene therapy; viral gene transfer system; non-viral or hybrid gene transfer systems; Sleeping Beauty (SB) transposon; NOD/SCID/gamma C(null) (NSG) mice; Non-integrating lentiviral (NIL) vectors; non-myeloablative conditioning; busulfan; fludarabine; relevant infant rhesus monkey bone marrow transplant (BMT) model

Record Details Similar Records Cite « Share

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Sumiyoshi, T. (2009). Non-viral and viral hematopoietic progenitor cell gene therapy. (Doctoral Dissertation). University of Southern California. Retrieved from http://digitallibrary.usc.edu/cdm/compoundobject/collection/p15799coll127/id/182101/rec/4447

Chicago Manual of Style (16^th Edition):

Sumiyoshi, Teiko. “Non-viral and viral hematopoietic progenitor cell gene therapy.” 2009. Doctoral Dissertation, University of Southern California. Accessed January 16, 2021. http://digitallibrary.usc.edu/cdm/compoundobject/collection/p15799coll127/id/182101/rec/4447.

MLA Handbook (7^th Edition):

Sumiyoshi, Teiko. “Non-viral and viral hematopoietic progenitor cell gene therapy.” 2009. Web. 16 Jan 2021.

Vancouver:

Sumiyoshi T. Non-viral and viral hematopoietic progenitor cell gene therapy. [Internet] [Doctoral dissertation]. University of Southern California; 2009. [cited 2021 Jan 16]. Available from: http://digitallibrary.usc.edu/cdm/compoundobject/collection/p15799coll127/id/182101/rec/4447.

Council of Science Editors:

Sumiyoshi T. Non-viral and viral hematopoietic progenitor cell gene therapy. [Doctoral Dissertation]. University of Southern California; 2009. Available from: http://digitallibrary.usc.edu/cdm/compoundobject/collection/p15799coll127/id/182101/rec/4447

Open Access Theses and Dissertations