subject:(transfection)

University of Alberta

1. Wong, Peter Kwok Pan. Exploring Novel Methods in Sonoportation.

Degree: MS, Department of Biomedical Engineering, 2012, University of Alberta

URL: https://era.library.ualberta.ca/files/2227mq96n

► Sonoporation, a physical, non-viral, non-chemical transfection method, promises great potentials. However, many drawbacks hinders its generalization. Low transfection rate and cell viability after treatment are… (more)

Subjects/Keywords: Delivery; Sonoporation; Transfection

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APA (6^th Edition):

Wong, P. K. P. (2012). Exploring Novel Methods in Sonoportation. (Masters Thesis). University of Alberta. Retrieved from https://era.library.ualberta.ca/files/2227mq96n

Chicago Manual of Style (16^th Edition):

Wong, Peter Kwok Pan. “Exploring Novel Methods in Sonoportation.” 2012. Masters Thesis, University of Alberta. Accessed January 23, 2021. https://era.library.ualberta.ca/files/2227mq96n.

MLA Handbook (7^th Edition):

Wong, Peter Kwok Pan. “Exploring Novel Methods in Sonoportation.” 2012. Web. 23 Jan 2021.

Vancouver:

Wong PKP. Exploring Novel Methods in Sonoportation. [Internet] [Masters thesis]. University of Alberta; 2012. [cited 2021 Jan 23]. Available from: https://era.library.ualberta.ca/files/2227mq96n.

Council of Science Editors:

Wong PKP. Exploring Novel Methods in Sonoportation. [Masters Thesis]. University of Alberta; 2012. Available from: https://era.library.ualberta.ca/files/2227mq96n

University of Hong Kong

2. Siu, King-sun. Development of crosslinked poly(ethylenimine) as a potential nucleic acid delivery agent.

Degree: 2009, University of Hong Kong

URL: http://hdl.handle.net/10722/130725

Subjects/Keywords: Polymers.; Transfection.

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APA (6^th Edition):

Siu, K. (2009). Development of crosslinked poly(ethylenimine) as a potential nucleic acid delivery agent. (Thesis). University of Hong Kong. Retrieved from http://hdl.handle.net/10722/130725

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Siu, King-sun. “Development of crosslinked poly(ethylenimine) as a potential nucleic acid delivery agent.” 2009. Thesis, University of Hong Kong. Accessed January 23, 2021. http://hdl.handle.net/10722/130725.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Siu, King-sun. “Development of crosslinked poly(ethylenimine) as a potential nucleic acid delivery agent.” 2009. Web. 23 Jan 2021.

Vancouver:

Siu K. Development of crosslinked poly(ethylenimine) as a potential nucleic acid delivery agent. [Internet] [Thesis]. University of Hong Kong; 2009. [cited 2021 Jan 23]. Available from: http://hdl.handle.net/10722/130725.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Siu K. Development of crosslinked poly(ethylenimine) as a potential nucleic acid delivery agent. [Thesis]. University of Hong Kong; 2009. Available from: http://hdl.handle.net/10722/130725

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Hong Kong

3. 黎永富. Fabrication and properties of poly(ethylenimine)-based polymers for non-viral plasmid delivery.

Degree: 2011, University of Hong Kong

URL: http://hdl.handle.net/10722/143215

Subjects/Keywords: Transfection.; Polymers.

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APA (6^th Edition):

黎永富. (2011). Fabrication and properties of poly(ethylenimine)-based polymers for non-viral plasmid delivery. (Thesis). University of Hong Kong. Retrieved from http://hdl.handle.net/10722/143215

Note: this citation may be lacking information needed for this citation format:
Author name may be incomplete
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

黎永富. “Fabrication and properties of poly(ethylenimine)-based polymers for non-viral plasmid delivery.” 2011. Thesis, University of Hong Kong. Accessed January 23, 2021. http://hdl.handle.net/10722/143215.

Note: this citation may be lacking information needed for this citation format:
Author name may be incomplete
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

黎永富. “Fabrication and properties of poly(ethylenimine)-based polymers for non-viral plasmid delivery.” 2011. Web. 23 Jan 2021.

Note: this citation may be lacking information needed for this citation format:
Author name may be incomplete

Vancouver:

黎永富. Fabrication and properties of poly(ethylenimine)-based polymers for non-viral plasmid delivery. [Internet] [Thesis]. University of Hong Kong; 2011. [cited 2021 Jan 23]. Available from: http://hdl.handle.net/10722/143215.

Note: this citation may be lacking information needed for this citation format:
Author name may be incomplete
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

黎永富. Fabrication and properties of poly(ethylenimine)-based polymers for non-viral plasmid delivery. [Thesis]. University of Hong Kong; 2011. Available from: http://hdl.handle.net/10722/143215

Note: this citation may be lacking information needed for this citation format:
Author name may be incomplete
Not specified: Masters Thesis or Doctoral Dissertation

Jawaharlal Nehru University

4. Prasad, T. K. Cell biology of lipid mediated transfection;.

Degree: Centre for Cellular and Molecular Biology, 2004, Jawaharlal Nehru University

URL: http://shodhganga.inflibnet.ac.in/handle/10603/16453

►

Gene delivery is a process by which an exogenous copy of the gene or DNA is newlineintroduced into the cell. Gene delivery has tremendous medical… (more)

Subjects/Keywords: Cell biology; mediated transfection

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APA (6^th Edition):

Prasad, T. K. (2004). Cell biology of lipid mediated transfection;. (Thesis). Jawaharlal Nehru University. Retrieved from http://shodhganga.inflibnet.ac.in/handle/10603/16453

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Prasad, T K. “Cell biology of lipid mediated transfection;.” 2004. Thesis, Jawaharlal Nehru University. Accessed January 23, 2021. http://shodhganga.inflibnet.ac.in/handle/10603/16453.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Prasad, T K. “Cell biology of lipid mediated transfection;.” 2004. Web. 23 Jan 2021.

Vancouver:

Prasad TK. Cell biology of lipid mediated transfection;. [Internet] [Thesis]. Jawaharlal Nehru University; 2004. [cited 2021 Jan 23]. Available from: http://shodhganga.inflibnet.ac.in/handle/10603/16453.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Prasad TK. Cell biology of lipid mediated transfection;. [Thesis]. Jawaharlal Nehru University; 2004. Available from: http://shodhganga.inflibnet.ac.in/handle/10603/16453

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Texas A&M University

5. Simeon, Rudo Lyndon. Selecting a path against Hepatitis C Virus.

Degree: PhD, Chemical Engineering, 2013, Texas A&M University

URL: http://hdl.handle.net/1969.1/151941

► Hepatitis C virus (HCV) currently affects ~5% of the world’s population and has relatively limited treatment options for infected patients. Genetic suppressor elements (GSE) derived… (more)

▼ Hepatitis C virus (HCV) currently affects ~5% of the world’s population and has relatively limited treatment options for infected patients. Genetic suppressor elements (GSE) derived from a gene or genome of interest can act as transdominant inhibitors of a particular biology function presumably by binding to and blocking an essential interaction surface for protein activity. Taking advantage of hepatoma cell line n4mBid, that supports all stages of the HCV life cycle and strongly report HCV infection by a cell-death phenotype, we developed an iterative selection/enrichment strategy for the identification of GSE against HCV. Using this strategy, a library expressing random fragments of the HCV genome was screened for sequences able to suppress HCV infection. A 244 amino acid gene fragment, B1, was strongly enriched after 5 rounds of selection. B1 has a very high net positive charge of 43 at neutral pH and a high charge-to-mass (kDa) ratio of 1.5. We show that B1 expression specifically inhibits HCV replication, apparently due to its high positive charge. We also show that recombinant positively charged proteins can inhibit HCV infection, when supplied in vitro.In addition, eGFP-fused B1 potently penetrates both adherent and suspension cells with >80% of cells taking up the protein. Importantly, we show that B1 not only facilitates cellular uptake, but allows protein cargo to reach sites of biological relevance. B1 also delivers non-covalently conjugated RNA and DNA across the cell membrane to cytosolic and nuclear sites, with efficiency comparable to commercially available cationic lipid reagents. Our data suggest that B1 utilizes cell-surface glycans and multiple competing endocytic pathways to enter and traffic through cells.During a separate screening carried out in our lab, we identified a TACR3 inhibitor SB 222200 that had significant HCV activity. We go on to show that both TACR1 and TACR3 receptors are expressed in the HCV-permissive Huh 7.5 cell line. We also show that both TACR1 and TACR3 inhibitors significantly inhibit HCV infection. These results point to the potential for TACR1 antagonists in treating patients infected with both HCV and HIV. Advisors/Committee Members: Chen, Zhilei (advisor), Jayaraman, Arul (committee member), Karim, Nazmul (committee member), Leibowitz, Julian (committee member).

Subjects/Keywords: HCV; biomolecules; biotherapeutics; transfection; transduction

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APA (6^th Edition):

Simeon, R. L. (2013). Selecting a path against Hepatitis C Virus. (Doctoral Dissertation). Texas A&M University. Retrieved from http://hdl.handle.net/1969.1/151941

Chicago Manual of Style (16^th Edition):

Simeon, Rudo Lyndon. “Selecting a path against Hepatitis C Virus.” 2013. Doctoral Dissertation, Texas A&M University. Accessed January 23, 2021. http://hdl.handle.net/1969.1/151941.

MLA Handbook (7^th Edition):

Simeon, Rudo Lyndon. “Selecting a path against Hepatitis C Virus.” 2013. Web. 23 Jan 2021.

Vancouver:

Simeon RL. Selecting a path against Hepatitis C Virus. [Internet] [Doctoral dissertation]. Texas A&M University; 2013. [cited 2021 Jan 23]. Available from: http://hdl.handle.net/1969.1/151941.

Council of Science Editors:

Simeon RL. Selecting a path against Hepatitis C Virus. [Doctoral Dissertation]. Texas A&M University; 2013. Available from: http://hdl.handle.net/1969.1/151941

Texas A&M University

6. Mintzer, Meredith Ann. The design and evaluation of triazine dendrimers for gene delivery.

Degree: PhD, Chemistry, 2011, Texas A&M University

URL: http://hdl.handle.net/1969.1/ETD-TAMU-2009-12-7330

► The interest in using gene therapy to target a variety of both inherited and acquired diseases has intensified over the last two decades. Because free… (more)

▼ The interest in using gene therapy to target a variety of both inherited and acquired diseases has intensified over the last two decades. Because free DNA is easily degraded by serum nucleases in the bloodstream, the need for developing carrier systems that can compact and protect the DNA was quickly realized. Viral vector systems were some of the earliest carriers used, primarily because of the ease with which such systems can infect host cells. However, difficulties experienced when using viral vectors, including immunogenicity and the potential for genetic recombinations, forced researchers to design alternative delivery strategies. Non-viral vectors offer one alternative to overcome this dilemma. In addition to avoiding the biological problems experienced using viral carriers, non-viral vectors also offer the potential for large-scale production. Dendrimers are one non-viral carrier that has shown appreciable ability to deliver DNA into cells, a process called transfection. In the past, triazine dendrimers have shown biocompatibility, and the ability to synthesize these structures to contain cationic charges on the surface makes these structures potentially suitable for transfection studies. In this study, a small library of triazine dendrimers was synthesized in an attempt to understand how variations to both the periphery and core of triazine dendrimers affect the transfection efficiency of these dendriplexes. In the first subset of structures, a common core was used and various peripheral groups were appended to the dendrimer surface. The physicochemical and biological data, obtained in collaboration with Thomas Kissel at Philipps-Universitat Marburg, showed that the surface groups have a notable affect on transfection efficiency. Dendrimers with a higher amine number and neutral surface groups show high DNA binding affinity and higher transfection efficiency. In the second subset of dendrimers, variations to the core showed that transfection efficiency is improved both by increasing generation number and dendrimer flexibility. With this data in hand, triazine dendrimers with both higher generation number and higher flexibility have been synthesized. Two different triazine linker groups, trimethylene-dipiperidine and polyglycoldiamine, have been used. These structures will be evaluated to determine if increasing both flexibility and generation number together can further improve transfection efficiency. Advisors/Committee Members: Simanek, Eric E. (advisor), Bergbreiter, David E. (committee member), Singleton, Daniel A. (committee member), Grunlan, Melissa A. (committee member).

Subjects/Keywords: triazine; dendrimer; transfection; DNA

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APA (6^th Edition):

Mintzer, M. A. (2011). The design and evaluation of triazine dendrimers for gene delivery. (Doctoral Dissertation). Texas A&M University. Retrieved from http://hdl.handle.net/1969.1/ETD-TAMU-2009-12-7330

Chicago Manual of Style (16^th Edition):

Mintzer, Meredith Ann. “The design and evaluation of triazine dendrimers for gene delivery.” 2011. Doctoral Dissertation, Texas A&M University. Accessed January 23, 2021. http://hdl.handle.net/1969.1/ETD-TAMU-2009-12-7330.

MLA Handbook (7^th Edition):

Mintzer, Meredith Ann. “The design and evaluation of triazine dendrimers for gene delivery.” 2011. Web. 23 Jan 2021.

Vancouver:

Mintzer MA. The design and evaluation of triazine dendrimers for gene delivery. [Internet] [Doctoral dissertation]. Texas A&M University; 2011. [cited 2021 Jan 23]. Available from: http://hdl.handle.net/1969.1/ETD-TAMU-2009-12-7330.

Council of Science Editors:

Mintzer MA. The design and evaluation of triazine dendrimers for gene delivery. [Doctoral Dissertation]. Texas A&M University; 2011. Available from: http://hdl.handle.net/1969.1/ETD-TAMU-2009-12-7330

University of Ottawa

7. McCann, Jenna. An Evaluation of Cell Transfection Strategies to Package RNA in Small Extracellular Vesicles .

Degree: 2020, University of Ottawa

URL: http://hdl.handle.net/10393/40234

► Small extracellular vesicles are endogenous delivery vehicles produced by most cells. They have been shown to carry miRNA to recipient cells to modulate them. As… (more)

▼ Small extracellular vesicles are endogenous delivery vehicles produced by most cells. They have been shown to carry miRNA to recipient cells to modulate them. As a result, they have attracted much attention for their potential use as delivery vehicles for therapeutics. Silencing RNAs are a potent drug class but toxicity from the delivery vehicles used and the elevated doses they require prevented many clinical trials from moving forward. Additionally, their therapeutic effect is short-lived but can be extended by the addition of chemical modifications. Therefore, it is beneficial to determine a method that enables chemically stabilized siRNAs to be packaged in small extracellular vesicles. Few miRNA are packaged in these vesicles and their selective packaging is not well understood, making it difficult to promote the loading of specific siRNAs into small extracellular vesicles. Since chemically modified RNAs cannot be expressed, transfection of extracellular vesicle-producing cells was attempted to load siRNA in small extracellular vesicles. Here, we provide evidence that small extracellular vesicle preparations isolated from transfected cells contain larger particles than ones isolated from untransfected cells, suggesting that the vesicles may fuse with transfection complexes. Transfected siRNA co-localized with the late endosomes where some vesicle subtypes, called exosomes, are synthesized. This suggests that transfection complexes may accumulate in the late endosome where they may fuse with or be released alongside small extracellular vesicles. Importantly, extracellular vesicles released by transfected cells also appear to be less efficient at delivering siRNA cargoes than those produced by untransfected cells. Therefore, transfection complexes or fusions of transfection complexes and the vesicles may be a major contaminant of the preparations of small extracellular vesicles from transfected cells. This is likely a major concern as a significant amount of published literature has used transfection of cells to load RNAs in small extracellular vesicles.

Subjects/Keywords: Extracellular Vesicles; Transfection Complexes

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APA (6^th Edition):

McCann, J. (2020). An Evaluation of Cell Transfection Strategies to Package RNA in Small Extracellular Vesicles . (Thesis). University of Ottawa. Retrieved from http://hdl.handle.net/10393/40234

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

McCann, Jenna. “An Evaluation of Cell Transfection Strategies to Package RNA in Small Extracellular Vesicles .” 2020. Thesis, University of Ottawa. Accessed January 23, 2021. http://hdl.handle.net/10393/40234.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

McCann, Jenna. “An Evaluation of Cell Transfection Strategies to Package RNA in Small Extracellular Vesicles .” 2020. Web. 23 Jan 2021.

Vancouver:

McCann J. An Evaluation of Cell Transfection Strategies to Package RNA in Small Extracellular Vesicles . [Internet] [Thesis]. University of Ottawa; 2020. [cited 2021 Jan 23]. Available from: http://hdl.handle.net/10393/40234.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

McCann J. An Evaluation of Cell Transfection Strategies to Package RNA in Small Extracellular Vesicles . [Thesis]. University of Ottawa; 2020. Available from: http://hdl.handle.net/10393/40234

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of British Columbia

8. Hogge, Donna Eileen. Genetic investigations of human hemopoiesis : studies of clonality and gene transfer to hemopoietic progenitors.

Degree: PhD, Pathology, 1987, University of British Columbia

URL: http://hdl.handle.net/2429/27316

► In most neoplasms malignant change occurs in a single cell which then proliferates. My purpose was to explore methods to study the cell that gives… (more)

▼ In most neoplasms malignant change occurs in a single cell which then proliferates. My purpose was to explore methods to study the cell that gives rise to hemopoietic cancer and to investigate the abnormalities at a molecular level. Cytogenetic analysis of cells from individual hemopoietic colonies revealed that monosomy 7 syndrome, a hematologic disorder of childhood, arises in a primitive cell capable of differentiating down both myeloid and erythroid pathways. Long-term bone marrow cultures (LTC) from patients with chronic myelogenous leukemia (CML) favor the growth of Philadelphia chromosome (Ph) negative progenitors which, although cytogenetically normal, could have been part of the malignant clone at a stage prior to the development of the Ph. LTC's were initiated with cells from 2 women with CML who were heterozygous for 2 electrophoretically distinct glucose-6-phosphate dehydrogenase (G6PD) enzyme variants. In one patient, 2/11 progenitors were Ph-negative after 4 to 6 weeks in LTC and 4/30 were nonclonal by G6PD enzyme analysis, i.e. the colonies expressed the enzyme not found in the malignant clone. In this case, karyotypically normal cells were truly normal. Next, gene transfer to human hemopoietic cells was demonstrated using recombinant retrovirus carrying the selectable marker gene, neor. With the K562 human leukemic cell line as targets up to 60% of infected cells became G418 resistant (G418r). Cloned populations of G418r cells showed unique patterns of retroviral integration in K562 DNA. When the target cells were progenitors from normal marrow, CML blood or fetal liver, the highest frequencies of G418r granulocyte-macrophage or large erythroid colonies was 16% and 5% respectively. Experiments infecting bone marrow cells in LTC with neor virus produced up to 2% G418r colonies after as long as 3 weeks in culture. Using v-src virus to infect LTC failed to perturb hemopoiesis, although infection of bone marrow-derived cells in these cultures was documented. In summary: 1. Unique populations of hemopoietic progenitors can be identified in culture using several genetic markers including chromosomes, G6PD analysis or gene transfer. 2. The feasibility of retroviral-mediated gene transfer for use on human hemopoietic cells has been demonstrated.

Subjects/Keywords: Transfection; Hematopoiesis

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Hogge, D. E. (1987). Genetic investigations of human hemopoiesis : studies of clonality and gene transfer to hemopoietic progenitors. (Doctoral Dissertation). University of British Columbia. Retrieved from http://hdl.handle.net/2429/27316

Chicago Manual of Style (16^th Edition):

Hogge, Donna Eileen. “Genetic investigations of human hemopoiesis : studies of clonality and gene transfer to hemopoietic progenitors.” 1987. Doctoral Dissertation, University of British Columbia. Accessed January 23, 2021. http://hdl.handle.net/2429/27316.

MLA Handbook (7^th Edition):

Hogge, Donna Eileen. “Genetic investigations of human hemopoiesis : studies of clonality and gene transfer to hemopoietic progenitors.” 1987. Web. 23 Jan 2021.

Vancouver:

Hogge DE. Genetic investigations of human hemopoiesis : studies of clonality and gene transfer to hemopoietic progenitors. [Internet] [Doctoral dissertation]. University of British Columbia; 1987. [cited 2021 Jan 23]. Available from: http://hdl.handle.net/2429/27316.

Council of Science Editors:

Hogge DE. Genetic investigations of human hemopoiesis : studies of clonality and gene transfer to hemopoietic progenitors. [Doctoral Dissertation]. University of British Columbia; 1987. Available from: http://hdl.handle.net/2429/27316

Texas State University – San Marcos

9. Palacios, Adriana S. Transfection Efficiencies of Chitosan/Sirna Nanoparticles in Colorectal Cancer Cells.

Degree: MS, Biochemistry, 2012, Texas State University – San Marcos

URL: https://digital.library.txstate.edu/handle/10877/5487

► RNA interference (RNAi) is a recently discovered phenomenon that employs the use of miRNA (microRNA) and siRNA (short interfering RNA) that can be utilized for… (more)

Subjects/Keywords: Transfection efficiency; siRNA; Chitosan nanoparticles

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APA (6^th Edition):

Palacios, A. S. (2012). Transfection Efficiencies of Chitosan/Sirna Nanoparticles in Colorectal Cancer Cells. (Masters Thesis). Texas State University – San Marcos. Retrieved from https://digital.library.txstate.edu/handle/10877/5487

Chicago Manual of Style (16^th Edition):

Palacios, Adriana S. “Transfection Efficiencies of Chitosan/Sirna Nanoparticles in Colorectal Cancer Cells.” 2012. Masters Thesis, Texas State University – San Marcos. Accessed January 23, 2021. https://digital.library.txstate.edu/handle/10877/5487.

MLA Handbook (7^th Edition):

Palacios, Adriana S. “Transfection Efficiencies of Chitosan/Sirna Nanoparticles in Colorectal Cancer Cells.” 2012. Web. 23 Jan 2021.

Vancouver:

Palacios AS. Transfection Efficiencies of Chitosan/Sirna Nanoparticles in Colorectal Cancer Cells. [Internet] [Masters thesis]. Texas State University – San Marcos; 2012. [cited 2021 Jan 23]. Available from: https://digital.library.txstate.edu/handle/10877/5487.

Council of Science Editors:

Palacios AS. Transfection Efficiencies of Chitosan/Sirna Nanoparticles in Colorectal Cancer Cells. [Masters Thesis]. Texas State University – San Marcos; 2012. Available from: https://digital.library.txstate.edu/handle/10877/5487

University of Ottawa

10. Cencen, Veronika. A Microfluidic Device for Transfection of Mammalian Cells Using Adjustable Shear Stress .

Degree: 2016, University of Ottawa

URL: http://hdl.handle.net/10393/35525

► Microfluidic technology is a rapidly progressing tool in biomedical engineering. Microfluidic devices are appreciated for their simplicity and production efficiency potential. Our research focuses on… (more)

Subjects/Keywords: Microfluidics; Transfection; Impedance; Photolithograpy; PAH; FITC

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APA (6^th Edition):

Cencen, V. (2016). A Microfluidic Device for Transfection of Mammalian Cells Using Adjustable Shear Stress . (Thesis). University of Ottawa. Retrieved from http://hdl.handle.net/10393/35525

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Cencen, Veronika. “A Microfluidic Device for Transfection of Mammalian Cells Using Adjustable Shear Stress .” 2016. Thesis, University of Ottawa. Accessed January 23, 2021. http://hdl.handle.net/10393/35525.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Cencen, Veronika. “A Microfluidic Device for Transfection of Mammalian Cells Using Adjustable Shear Stress .” 2016. Web. 23 Jan 2021.

Vancouver:

Cencen V. A Microfluidic Device for Transfection of Mammalian Cells Using Adjustable Shear Stress . [Internet] [Thesis]. University of Ottawa; 2016. [cited 2021 Jan 23]. Available from: http://hdl.handle.net/10393/35525.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Cencen V. A Microfluidic Device for Transfection of Mammalian Cells Using Adjustable Shear Stress . [Thesis]. University of Ottawa; 2016. Available from: http://hdl.handle.net/10393/35525

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Boston University

11. Hayward, Oliver James. Mechanisms of Mononegavirales gene expression.

Degree: MS, Medical Sciences, 2019, Boston University

URL: http://hdl.handle.net/2144/38661

► The Mononegavirales order unifies the non-segmented negative sense viruses (nsNSVs), including Marburgvirus (MARV) of the Filoviridae family and respiratory syncytial virus (RSV) of the Pneumoviridae.… (more)

▼ The Mononegavirales order unifies the non-segmented negative sense viruses (nsNSVs), including Marburgvirus (MARV) of the Filoviridae family and respiratory syncytial virus (RSV) of the Pneumoviridae. The mechanism of action of these viruses and how they infect cells are very similar, especially when focusing on their polymerases, which are distinct from those of eukaryotes and therefore possible targets for antiviral drugs. nsNSVs utilize a RNA-dependent RNA polymerase to either replicate the viral RNA genome or transcribe it into positive sense mRNA. Despite this, these two viruses result in very different, but equally devastating, effects in humans. Whereas MARV virus often results in rare but fatal hemorrhagic fevers, RSV is a common seasonal virus that can result in long term negative effects to respiratory health. These negative effects on public health demand extensive research in these two fields and a need to develop new technology and methods in order to uncover the missing pieces of viral gene expression. Specifically, the development of a MARV minigenome system would allow for increased testing of this virus outside of the confines of the biosafety level 4 (BSL-4) setting. By replacing MARV genes with reporter genes, but retaining the characteristic leader, intergenic, and trailer regions of the genome, tests involving site directed mutagenesis would reveal new insights into the crucial genomic elements needed for successful gene expression. Coupled with the transfection of the minigenome with plasmids coding for the crucial MARV proteins, artificial changes to the genome would lead to the presence of absence of translated bioluminescent reporter proteins. Using these two viruses, this study attempted to find commonalities across families. Specifically, the goals of this research were twofold, to find the optimal ratio of MARV plasmids in the minigenome system to understand the effects of the stem-loop secondary structure of MARV mRNA transcripts as well as determine the tail length of the poly(A) tail of RSV mRNA transcripts using digestion and probing primers. Calculating the RSV poly(A) tail length would allow for further research into determining whether the MARV and RSV polymerase polyadenylates before or after it releases the transcript. Despite multiple failed attempts, transfections using pCAGGS plasmids and the eGFP monocistronic minigenome in a 6-well plate qualitatively demonstrated the need for pCAGGS-L plasmid concentration of 1000 ng/µl. Due to time constraints, the poly(A) tail length of the RSV NS-1 mRNA transcript could not be determined. Overall, this study focused on gaining new insights on the techniques and procedures necessary for conducting virus research in a biosafety level 2 (BSL-2) setting, as well as developing troubleshooting skills in approaching fail experiments. Advisors/Committee Members: Fearns, Rachel (advisor), Muhlberger, Elke (advisor).

Subjects/Keywords: Medicine; Marburg; Minigenome; Polyadenylation; Respiratory; Syncytial; Transfection

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APA (6^th Edition):

Hayward, O. J. (2019). Mechanisms of Mononegavirales gene expression. (Masters Thesis). Boston University. Retrieved from http://hdl.handle.net/2144/38661

Chicago Manual of Style (16^th Edition):

Hayward, Oliver James. “Mechanisms of Mononegavirales gene expression.” 2019. Masters Thesis, Boston University. Accessed January 23, 2021. http://hdl.handle.net/2144/38661.

MLA Handbook (7^th Edition):

Hayward, Oliver James. “Mechanisms of Mononegavirales gene expression.” 2019. Web. 23 Jan 2021.

Vancouver:

Hayward OJ. Mechanisms of Mononegavirales gene expression. [Internet] [Masters thesis]. Boston University; 2019. [cited 2021 Jan 23]. Available from: http://hdl.handle.net/2144/38661.

Council of Science Editors:

Hayward OJ. Mechanisms of Mononegavirales gene expression. [Masters Thesis]. Boston University; 2019. Available from: http://hdl.handle.net/2144/38661

Hong Kong University of Science and Technology

12. Gao, Wenhui. Study of DNA-uptake mechanisms in electroporation.

Degree: 1998, Hong Kong University of Science and Technology

URL: http://repository.ust.hk/ir/Record/1783.1-3705 ; https://doi.org/10.14711/thesis-b589613 ; http://repository.ust.hk/ir/bitstream/1783.1-3705/1/th_redirect.html

► Electroporation is one of the most common methods used to transfer exogenous gene into most prokaryotic or eukaryotic cells. The detailed mechanisms of DNA-uptake by… (more)

▼ Electroporation is one of the most common methods used to transfer exogenous gene into most prokaryotic or eukaryotic cells. The detailed mechanisms of DNA-uptake by cells, however, have not yet been clearly understood. In this thesis, we have summarized a series of experiment designed to examine the DAN- uptake mechanisms. In addition, cell treatments that can enhance the efficiency of electroporation are also discussed. The results show that after the application of electric field the additional treatment by using a recovery medium containing proper amounts of divalent ions can significantly increase the transfection efficiency in 3T6, Pt K2 and SK-N-SH cells. Several factors, including the strength of electric field, recovery time and concentrations of Ca++ or Mg++ were examined. The reason for increasing the transfection efficiency is believed to be mainly caused by promoting the entry of DNA through the cell membrane, rather than by enhancing the expression of the transferred gene. It has been suggested that DNA-uptake is a multiple- step process. (1) DNA may be driven into the cell by an electrophoretic force when the membrane pores were opened during the application of the electric field. (2) After the electric field was turned off, DNA may continue to diffuse into the cell before the membrane pores were closed. (3) Finally, DNA may be carried in the cell by endocytosis after the membrane pores were resealed. In this research, we have designed specific experiments to test each of these steps. Our results support steps (1) and (3), but not step (2). We found that the kinetics of membrane pore resealing depends strongly on the size of the transport molecules. Thus, uptake of large molecules such as FITC labeled dextran (70 kD) or DNA stopped almost within seconds after the electric field was turned off. In summary, we propose that the DNA-uptake mechanisms in electroporation involve a fast step of DNA entrance driven by the electric field, and a relatively slower process of DNA-uptake by endocytosis following the turn-off of the electric pulses. The experimental evidence will be discussed in detail in this thesis.

Subjects/Keywords: Electroporation ; DNA ; Transfection

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APA (6^th Edition):

Gao, W. (1998). Study of DNA-uptake mechanisms in electroporation. (Thesis). Hong Kong University of Science and Technology. Retrieved from http://repository.ust.hk/ir/Record/1783.1-3705 ; https://doi.org/10.14711/thesis-b589613 ; http://repository.ust.hk/ir/bitstream/1783.1-3705/1/th_redirect.html

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Gao, Wenhui. “Study of DNA-uptake mechanisms in electroporation.” 1998. Thesis, Hong Kong University of Science and Technology. Accessed January 23, 2021. http://repository.ust.hk/ir/Record/1783.1-3705 ; https://doi.org/10.14711/thesis-b589613 ; http://repository.ust.hk/ir/bitstream/1783.1-3705/1/th_redirect.html.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Gao, Wenhui. “Study of DNA-uptake mechanisms in electroporation.” 1998. Web. 23 Jan 2021.

Vancouver:

Gao W. Study of DNA-uptake mechanisms in electroporation. [Internet] [Thesis]. Hong Kong University of Science and Technology; 1998. [cited 2021 Jan 23]. Available from: http://repository.ust.hk/ir/Record/1783.1-3705 ; https://doi.org/10.14711/thesis-b589613 ; http://repository.ust.hk/ir/bitstream/1783.1-3705/1/th_redirect.html.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Gao W. Study of DNA-uptake mechanisms in electroporation. [Thesis]. Hong Kong University of Science and Technology; 1998. Available from: http://repository.ust.hk/ir/Record/1783.1-3705 ; https://doi.org/10.14711/thesis-b589613 ; http://repository.ust.hk/ir/bitstream/1783.1-3705/1/th_redirect.html

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Minnesota

13. Phillips, Haley. Elucidating Glycopolycation Structure-Function Relationships For Improved Gene Therapy.

Degree: PhD, Chemistry, 2017, University of Minnesota

URL: http://hdl.handle.net/11299/206410

► The gene therapy field is devoted to treating disease by adding, altering, or inhibiting gene expression. This type of therapy holds great promise for the… (more)

Subjects/Keywords: Biocompatibility; Delivery; Glycopolymer; Nonviral; Polyplex; Transfection

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APA (6^th Edition):

Phillips, H. (2017). Elucidating Glycopolycation Structure-Function Relationships For Improved Gene Therapy. (Doctoral Dissertation). University of Minnesota. Retrieved from http://hdl.handle.net/11299/206410

Chicago Manual of Style (16^th Edition):

Phillips, Haley. “Elucidating Glycopolycation Structure-Function Relationships For Improved Gene Therapy.” 2017. Doctoral Dissertation, University of Minnesota. Accessed January 23, 2021. http://hdl.handle.net/11299/206410.

MLA Handbook (7^th Edition):

Phillips, Haley. “Elucidating Glycopolycation Structure-Function Relationships For Improved Gene Therapy.” 2017. Web. 23 Jan 2021.

Vancouver:

Phillips H. Elucidating Glycopolycation Structure-Function Relationships For Improved Gene Therapy. [Internet] [Doctoral dissertation]. University of Minnesota; 2017. [cited 2021 Jan 23]. Available from: http://hdl.handle.net/11299/206410.

Council of Science Editors:

Phillips H. Elucidating Glycopolycation Structure-Function Relationships For Improved Gene Therapy. [Doctoral Dissertation]. University of Minnesota; 2017. Available from: http://hdl.handle.net/11299/206410

University of Minnesota

14. Boyle, William. Charged Glycopolymer Materials for Epidermolysis Bullosa.

Degree: PhD, Chemistry, 2017, University of Minnesota

URL: http://hdl.handle.net/11299/201073

► Improved delivery of therapeutic nucleic acid payloads to cells could lead to dramatically improved clinical outcomes for patients suffering from genetic disorders. This work focuses… (more)

Subjects/Keywords: Epidermolysis Bullosa; Gene Delivery; Polymers; Transfection

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APA (6^th Edition):

Boyle, W. (2017). Charged Glycopolymer Materials for Epidermolysis Bullosa. (Doctoral Dissertation). University of Minnesota. Retrieved from http://hdl.handle.net/11299/201073

Chicago Manual of Style (16^th Edition):

Boyle, William. “Charged Glycopolymer Materials for Epidermolysis Bullosa.” 2017. Doctoral Dissertation, University of Minnesota. Accessed January 23, 2021. http://hdl.handle.net/11299/201073.

MLA Handbook (7^th Edition):

Boyle, William. “Charged Glycopolymer Materials for Epidermolysis Bullosa.” 2017. Web. 23 Jan 2021.

Vancouver:

Boyle W. Charged Glycopolymer Materials for Epidermolysis Bullosa. [Internet] [Doctoral dissertation]. University of Minnesota; 2017. [cited 2021 Jan 23]. Available from: http://hdl.handle.net/11299/201073.

Council of Science Editors:

Boyle W. Charged Glycopolymer Materials for Epidermolysis Bullosa. [Doctoral Dissertation]. University of Minnesota; 2017. Available from: http://hdl.handle.net/11299/201073

Duke University

15. Wu, Mina. Implicated Role of Endocytosis in the Internalization and Intracellular Transport of Plasmid DNA During Electric Field-Mediated Gene Delivery .

Degree: 2011, Duke University

URL: http://hdl.handle.net/10161/4994

► Electric field mediated gene delivery (EFMGD) or electrotransfection is a popular, non-viral gene delivery method that has been used in a variety of studies… (more)

▼ Electric field mediated gene delivery (EFMGD) or electrotransfection is a popular, non-viral gene delivery method that has been used in a variety of studies and applications ranging from basic cell biology research to clinical gene therapy. Yet, the mechanism(s) by which electrotransfection facilitates DNA delivery across the cell membrane into the cell and its subsequent intracellular transport across the cytosolic space towards the nucleus have been insufficiently studied and still remain controversial. Understanding these mechanisms and characterizing the intracellular journey of pDNA is important for understanding the physiological barriers of EFMGD within the cell, which can be used to engineer better solutions to overcome these barriers with the ultimate goal of improving the transfection efficiency of this technology. Conventional thought in the field assumes that such transport modes as diffusion, electrophoresis, and electro-osmosis, which govern the entry of small molecules into cells through electric field-generated transient membrane pores, also apply to electric field-mediated delivery of therapeutic DNA. We propose that electrically-induced gene transfer into cells is governed by an alternative, more active mode of transport that entails the involvement of cellular endocytic processes. It is our hypothesis that pulsed electric field generate these membrane pores which interact with nearby DNA molecules; but that actual DNA translocation across the membrane is driven by endocytosis, which consequently, then, also plays a role in the intracellular transport of the DNA. To this end, we first investigated the dependence of electrotransfection efficiency (eTE) on binding of plasmid DNA (pDNA) to plasma membrane. Binding concentrates DNA molecules in the vicinity of the cell membrane, which should theoretically result in a greater number of DNA-membrane interactions during pulsed electric field, more internalized DNA, and ultimately, higher eTE values. We demonstrated that supplementing the electrotransfection buffer with divalent cations (Ca2+ and Mg2+) is an effective method of promoting pDNA adsorption to the cell membrane. This cation-mediated increase in DNA adsorption to the cellular membrane resulted in a consequent increase in eTE, up to a certain threshold concentration for each cation. To determine the timeframe for completion of pDNA internalization following pulse treatment, trypsin treatment was applied to cells at different timepoints after electrotransfection to strip off any residual, membrane-bound pDNA that had not been internalized. Trypsin treatment at 10 min post electrotransfection still resulted in a significant reduction in eTE, indicating that the time period for complete cellular uptake far exceeded the lifetime (~ 10 msec) of electric field-induced transient pores. The role of endocytosis was further probed by noting the effect on eTE when cells were treated with three endocytic inhibitors (chlorpromazine, genistein, dynasore) targeting different internalization… Advisors/Committee Members: Yuan, Fan (advisor).

Subjects/Keywords: Biomedical engineering; Electric Field; Gene Delivery; Transfection

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Wu, M. (2011). Implicated Role of Endocytosis in the Internalization and Intracellular Transport of Plasmid DNA During Electric Field-Mediated Gene Delivery . (Thesis). Duke University. Retrieved from http://hdl.handle.net/10161/4994

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Wu, Mina. “Implicated Role of Endocytosis in the Internalization and Intracellular Transport of Plasmid DNA During Electric Field-Mediated Gene Delivery .” 2011. Thesis, Duke University. Accessed January 23, 2021. http://hdl.handle.net/10161/4994.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Wu, Mina. “Implicated Role of Endocytosis in the Internalization and Intracellular Transport of Plasmid DNA During Electric Field-Mediated Gene Delivery .” 2011. Web. 23 Jan 2021.

Vancouver:

Wu M. Implicated Role of Endocytosis in the Internalization and Intracellular Transport of Plasmid DNA During Electric Field-Mediated Gene Delivery . [Internet] [Thesis]. Duke University; 2011. [cited 2021 Jan 23]. Available from: http://hdl.handle.net/10161/4994.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Wu M. Implicated Role of Endocytosis in the Internalization and Intracellular Transport of Plasmid DNA During Electric Field-Mediated Gene Delivery . [Thesis]. Duke University; 2011. Available from: http://hdl.handle.net/10161/4994

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

16. Shuang , Liang. New Approach To DNA Transfection And Genetics In Schistosome Parasites.

Degree: PhD, Biology, 2015, Case Western Reserve University School of Graduate Studies

URL: http://rave.ohiolink.edu/etdc/view?acc_num=case1424201510

► Schistosome worms of the genus Schistosoma are the causative agents of schistosomiasis, a serious global disease affecting more than 240 million people worldwide. The complex… (more)

Subjects/Keywords: Molecular Biology; Parasitology; schistosome; transfection; overexpression; promoter

10 more images…

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APA (6^th Edition):

Shuang , L. (2015). New Approach To DNA Transfection And Genetics In Schistosome Parasites. (Doctoral Dissertation). Case Western Reserve University School of Graduate Studies. Retrieved from http://rave.ohiolink.edu/etdc/view?acc_num=case1424201510

Chicago Manual of Style (16^th Edition):

Shuang , Liang. “New Approach To DNA Transfection And Genetics In Schistosome Parasites.” 2015. Doctoral Dissertation, Case Western Reserve University School of Graduate Studies. Accessed January 23, 2021. http://rave.ohiolink.edu/etdc/view?acc_num=case1424201510.

MLA Handbook (7^th Edition):

Shuang , Liang. “New Approach To DNA Transfection And Genetics In Schistosome Parasites.” 2015. Web. 23 Jan 2021.

Vancouver:

Shuang L. New Approach To DNA Transfection And Genetics In Schistosome Parasites. [Internet] [Doctoral dissertation]. Case Western Reserve University School of Graduate Studies; 2015. [cited 2021 Jan 23]. Available from: http://rave.ohiolink.edu/etdc/view?acc_num=case1424201510.

Council of Science Editors:

Shuang L. New Approach To DNA Transfection And Genetics In Schistosome Parasites. [Doctoral Dissertation]. Case Western Reserve University School of Graduate Studies; 2015. Available from: http://rave.ohiolink.edu/etdc/view?acc_num=case1424201510

17. Pérard, Matthieu. Mise au point d’un modèle tridimensionnel de culture d’odontoblastes. Application à l’évaluation in vitro de biomatériaux. : Development of a three-dimensional model of odontoblast culture. Application to an in vitro evaluation of biomaterials.

Degree: Docteur es, Biologie et sciences de la santé, 2015, Rennes 1

URL: http://www.theses.fr/2015REN1B001

►

L’objectif de ce travail était de mettre au point un modèle de culture cellulaire, afin d’étudier in vitro l’incidence sur la physiologie des cellules pulpaires… (more)

Subjects/Keywords: Sphéroïdes; Cellules pulpaires; Minéralisation; Coiffage pulpaire; Transfection; Spheroids; Pulp cells; Mineralisation; Pulp capping; Transfection

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Pérard, M. (2015). Mise au point d’un modèle tridimensionnel de culture d’odontoblastes. Application à l’évaluation in vitro de biomatériaux. : Development of a three-dimensional model of odontoblast culture. Application to an in vitro evaluation of biomaterials. (Doctoral Dissertation). Rennes 1. Retrieved from http://www.theses.fr/2015REN1B001

Chicago Manual of Style (16^th Edition):

Pérard, Matthieu. “Mise au point d’un modèle tridimensionnel de culture d’odontoblastes. Application à l’évaluation in vitro de biomatériaux. : Development of a three-dimensional model of odontoblast culture. Application to an in vitro evaluation of biomaterials.” 2015. Doctoral Dissertation, Rennes 1. Accessed January 23, 2021. http://www.theses.fr/2015REN1B001.

MLA Handbook (7^th Edition):

Pérard, Matthieu. “Mise au point d’un modèle tridimensionnel de culture d’odontoblastes. Application à l’évaluation in vitro de biomatériaux. : Development of a three-dimensional model of odontoblast culture. Application to an in vitro evaluation of biomaterials.” 2015. Web. 23 Jan 2021.

Vancouver:

Pérard M. Mise au point d’un modèle tridimensionnel de culture d’odontoblastes. Application à l’évaluation in vitro de biomatériaux. : Development of a three-dimensional model of odontoblast culture. Application to an in vitro evaluation of biomaterials. [Internet] [Doctoral dissertation]. Rennes 1; 2015. [cited 2021 Jan 23]. Available from: http://www.theses.fr/2015REN1B001.

Council of Science Editors:

Pérard M. Mise au point d’un modèle tridimensionnel de culture d’odontoblastes. Application à l’évaluation in vitro de biomatériaux. : Development of a three-dimensional model of odontoblast culture. Application to an in vitro evaluation of biomaterials. [Doctoral Dissertation]. Rennes 1; 2015. Available from: http://www.theses.fr/2015REN1B001

Brno University of Technology

18. Pošustová, Veronika. Transfekce somatických buněk pomocí magnetických nanočástic a jejich post-transfekční lokalizace: Transfection of somatic cells using magnetic nanoparticles and their post-transfection localization.

Degree: 2018, Brno University of Technology

URL: http://hdl.handle.net/11012/82553

► This bachelor thesis is focused on an experiment based on magnetic particles SPIO for transfection of 3T3 cells. Nanoparticle surface was modified with PEI and… (more)

Subjects/Keywords: Nanočástice; magnetické nanočástice; transfekce; transfekční efektivita; Nanoparticles; magnetic nanoparticles; transfection; transfection efficiency

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APA (6^th Edition):

Pošustová, V. (2018). Transfekce somatických buněk pomocí magnetických nanočástic a jejich post-transfekční lokalizace: Transfection of somatic cells using magnetic nanoparticles and their post-transfection localization. (Thesis). Brno University of Technology. Retrieved from http://hdl.handle.net/11012/82553

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Pošustová, Veronika. “Transfekce somatických buněk pomocí magnetických nanočástic a jejich post-transfekční lokalizace: Transfection of somatic cells using magnetic nanoparticles and their post-transfection localization.” 2018. Thesis, Brno University of Technology. Accessed January 23, 2021. http://hdl.handle.net/11012/82553.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Pošustová, Veronika. “Transfekce somatických buněk pomocí magnetických nanočástic a jejich post-transfekční lokalizace: Transfection of somatic cells using magnetic nanoparticles and their post-transfection localization.” 2018. Web. 23 Jan 2021.

Vancouver:

Pošustová V. Transfekce somatických buněk pomocí magnetických nanočástic a jejich post-transfekční lokalizace: Transfection of somatic cells using magnetic nanoparticles and their post-transfection localization. [Internet] [Thesis]. Brno University of Technology; 2018. [cited 2021 Jan 23]. Available from: http://hdl.handle.net/11012/82553.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Pošustová V. Transfekce somatických buněk pomocí magnetických nanočástic a jejich post-transfekční lokalizace: Transfection of somatic cells using magnetic nanoparticles and their post-transfection localization. [Thesis]. Brno University of Technology; 2018. Available from: http://hdl.handle.net/11012/82553

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

19. Sigwalt, David. Conception, synthèse et étude de dérivés de C60 fonctionnalisés : applications biologiques et développement méthodologique : Design, synthesis and study of functionalized C60 : biological applications and methodological development.

Degree: Docteur es, Chimie, 2013, Université de Strasbourg

URL: http://www.theses.fr/2013STRAF010

►

Notre équipe a récemment développé une méthode polyvalente permettant de préparer des dérivés complexes de C60 hexa-adduits fonctionnalisés. Cette méthodologie permet d’obtenir des produits aux… (more)

▼

Notre équipe a récemment développé une méthode polyvalente permettant de préparer des dérivés complexes de C60 hexa-adduits fonctionnalisés. Cette méthodologie permet d’obtenir des produits aux caractéristiques originales. Le C60 central agit comme un support central peu réactif, autour duquel des fonctionnalités sont réparties dans un espace octaédrique parfaitement défini. La première partie de ce travail de thèse a consisté à exploiter cette méthodologie pour créer des C60 hexa-adduits polycationiques aux propriétés de transfection remarquables. Dans un second temps, les dendrons polyamines synthétisés ont été mis à profit pour créer des structures supramoléculaires de C60 hexa-adduits, sous forme micellaire. Par la suite, l’étude de ces assemblages a orienté nos investigations vers l’élaboration de dérivés de C60 hexa-adduits mannosylés multivalents résultant d’un assemblage supramoléculaire, dont leurs possibles applications biologiques sont actuellement à l’étude. En parallèle une synthèse covalente a permis d’obtenir un «équivalent dendritique» de C60 hexa-adduit multimannosylé. Partant du constat que notre méthodologie est efficace principalement pour des dérivés de C60 hexa-adduits qui ont une régio-sélectivité particulière, la dernière partie a été consacrée au développement de nouvelles voies de synthèses qui pourront permettre de créer des dérivés de C60 avec un contrôle régio-sélectif original.

Our team has recently built a polyvalent method that gives complex functionalized C60 hexa-adducts. This methodology permits to obtain products with original features. C60 acts as an inert scaffold, around which functionnalities are distributed in a well-defined octahedral space. The first part of this thesis describes exploitation of this methodology to create polycationics C60 hexa-adducts, which have shown remarkable gene delivery capabilities. Next, the synthetized polyamine dendrons were used to build supramolecular structure of C60 hexa-adducts, as micellar forms. The study of these self-assembled structures has guided us to design micelles of mannosylated C60 hexa-adducts, which biological applications are under investigation. In parallel a covalent synthesis has furnished a dendrimers-like multimannosylated C60 hexa-adduct. Based on the observation that our methodology to create C60 hexa-adduct is efficient only for a specific regio-selectivity, the last part of this thesis was devoted to the development of new synthetic routes to obtain C60 derivates with an original regio-selective control.

Advisors/Committee Members: Nierengarten, Jean-François (thesis director), Remy, Jean-Serge (thesis director).

Subjects/Keywords: C60; Dendrimer; Amphiphile; Micelles; Polydiacétylènes; Transfection; Auto-assemblages; C60; Dendrimers; Amphiphiles; Micelles; Polydiacetylenes; Transfection; Self-assembling; 547.2

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APA (6^th Edition):

Sigwalt, D. (2013). Conception, synthèse et étude de dérivés de C60 fonctionnalisés : applications biologiques et développement méthodologique : Design, synthesis and study of functionalized C60 : biological applications and methodological development. (Doctoral Dissertation). Université de Strasbourg. Retrieved from http://www.theses.fr/2013STRAF010

Chicago Manual of Style (16^th Edition):

Sigwalt, David. “Conception, synthèse et étude de dérivés de C60 fonctionnalisés : applications biologiques et développement méthodologique : Design, synthesis and study of functionalized C60 : biological applications and methodological development.” 2013. Doctoral Dissertation, Université de Strasbourg. Accessed January 23, 2021. http://www.theses.fr/2013STRAF010.

MLA Handbook (7^th Edition):

Sigwalt, David. “Conception, synthèse et étude de dérivés de C60 fonctionnalisés : applications biologiques et développement méthodologique : Design, synthesis and study of functionalized C60 : biological applications and methodological development.” 2013. Web. 23 Jan 2021.

Vancouver:

Sigwalt D. Conception, synthèse et étude de dérivés de C60 fonctionnalisés : applications biologiques et développement méthodologique : Design, synthesis and study of functionalized C60 : biological applications and methodological development. [Internet] [Doctoral dissertation]. Université de Strasbourg; 2013. [cited 2021 Jan 23]. Available from: http://www.theses.fr/2013STRAF010.

Council of Science Editors:

Sigwalt D. Conception, synthèse et étude de dérivés de C60 fonctionnalisés : applications biologiques et développement méthodologique : Design, synthesis and study of functionalized C60 : biological applications and methodological development. [Doctoral Dissertation]. Université de Strasbourg; 2013. Available from: http://www.theses.fr/2013STRAF010

20. Morin-Picardat, Emmanuelle. Etudes d'auto-assemblages polydiacétylèniques et applications biologiques : Polydiacetylenic self-assemblies and biological applications.

Degree: Docteur es, Chimie, 2012, Université de Strasbourg

URL: http://www.theses.fr/2012STRAF063

►

La dualité hydrophobe/hydrophile des molécules amphiphiles est à l’origine de leur autoassemblage en solution, sous forme de nombreuses structures supramoléculaires, telles que les micelles. Ce… (more)

▼

La dualité hydrophobe/hydrophile des molécules amphiphiles est à l’origine de leur autoassemblage en solution, sous forme de nombreuses structures supramoléculaires, telles que les micelles. Ce travail de thèse présente la formation, la caractérisation et l’étude de nouvelles micelles diacétylèniques photopolymérisables. Une première partie décrit ainsi lasynthèse de nouvelles micelles cationiques et l’étude de leur utilisation en tant qu’agent de transfert de gènes. Dans une seconde partie, nos travaux présentent l’étude de micelles polydiacétylèniques, porteuses de têtes polaires octaéthylèneglycol, comme potentiel système de délivrance de médicament. Les propriétés d’encapsulation de ces micelles ont été évaluées en présence d’un dérivé fullerène fluorescent. Puis, l’incorporation d’une sonde membranaire dans leur couronne lipophile a permis de réaliser une étude de leurs propriétés de délivrance in vitro. Une étude préliminaire de leur biodistribution in vivo a également été réalisée par tomographie à émission monophotonique grâce à la chélation d’un isotoperadioactif sur la surface des micelles. Enfin une dernière partie présente l’analyse de deux nouveaux auto-assemblages tubulaires obtenus au cours de nos travaux.

The hydrophobic/hydrophilic duality of amphiphilic molecules is at the origin of their selfassembly in solution, into many supramolecular structures such as micelles. This thesis presents the formation, characterization and study of new photopolymerizable diacetylenic micelles. The first part describes the synthesis of new cationic micelles and the study of theiruse as gene transfer agent. In the second part, our work presents the study of polydiacetylene micelles, bearing polar heads octaétylèneglycol as potential drug delivery system. The encapsulation properties of these micelles were first evaluated in the presence of a fluorescent fullerene derivative. Then, the incorporation of a membrane probe in their lipophilic shell has allowed a study of their in vitro delivery properties. Finally a study of their in vivo biodistribution was also carried out by single photon emission tomography through the chelation of a radioactive isotope on micelle surface. Finally the last part presents theanalysis of two new tubularself-assemblies, obtained during our work.

Advisors/Committee Members: Remy, Jean-Serge (thesis director), Wagner, Alain (thesis director).

Subjects/Keywords: Amphiphiles; Micelles; Polydiacétylènes; Transfection; Délivrance de médicaments; Auto-assemblages; Amphiphiles; Micelles; Polydiacetylenes; Transfection; Drug delivery; Self-assembling; 547.8

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Morin-Picardat, E. (2012). Etudes d'auto-assemblages polydiacétylèniques et applications biologiques : Polydiacetylenic self-assemblies and biological applications. (Doctoral Dissertation). Université de Strasbourg. Retrieved from http://www.theses.fr/2012STRAF063

Chicago Manual of Style (16^th Edition):

Morin-Picardat, Emmanuelle. “Etudes d'auto-assemblages polydiacétylèniques et applications biologiques : Polydiacetylenic self-assemblies and biological applications.” 2012. Doctoral Dissertation, Université de Strasbourg. Accessed January 23, 2021. http://www.theses.fr/2012STRAF063.

MLA Handbook (7^th Edition):

Morin-Picardat, Emmanuelle. “Etudes d'auto-assemblages polydiacétylèniques et applications biologiques : Polydiacetylenic self-assemblies and biological applications.” 2012. Web. 23 Jan 2021.

Vancouver:

Morin-Picardat E. Etudes d'auto-assemblages polydiacétylèniques et applications biologiques : Polydiacetylenic self-assemblies and biological applications. [Internet] [Doctoral dissertation]. Université de Strasbourg; 2012. [cited 2021 Jan 23]. Available from: http://www.theses.fr/2012STRAF063.

Council of Science Editors:

Morin-Picardat E. Etudes d'auto-assemblages polydiacétylèniques et applications biologiques : Polydiacetylenic self-assemblies and biological applications. [Doctoral Dissertation]. Université de Strasbourg; 2012. Available from: http://www.theses.fr/2012STRAF063

21. Sabraoui, Abbas. Régulation de la cavitation acoustique appliquée à la transfection cellulaire : Feedback loop process to control acoustic cavitation : application to cell transfection.

Degree: Docteur es, Acoustique, 2012, Université Claude Bernard – Lyon I

URL: http://www.theses.fr/2012LYO10007

►

Le travail présenté ici porte sur l’étude et le contrôle de la cavitation acoustique dans le but de développer un système de sonoporation efficace pour… (more)

▼

Le travail présenté ici porte sur l’étude et le contrôle de la cavitation acoustique dans le but de développer un système de sonoporation efficace pour les cellules en suspension et les cellules adhérentes. Le manuscrit est composé de trois chapitres. Tout d’abord, une revue de la littérature sur les différentes techniques physiques utilisées en transfection cellulaire, et plus particulièrement la sonoporation. Il a été démontré que le principal mécanisme de la sonoporation est étroitement lié au phénomène de cavitation acoustique. Un contrôle de ce phénomène aléatoire apparaît alors intéressant afin d’augmenter le taux de transfection tout en gardant une forte viabilité cellulaire. Dans le second chapitre, un système de régulation de cavitation ultrasonore basé sur un indice acoustique de cavitation a été étudié. Cet indice, est basé sur la mesure de bruit large bande émis lors de l’implosion des bulles de cavitation. Les avantages d’un tel système sont : un suivi en temps réel du niveau de cavitation durant l’irradiation, des informations quasi-instantanées sur les composantes spectrales caractéristiques de la cavitation, une meilleure reproductibilité et stabilité du niveau de cavitation surtout pour les intensités modérés. Dans le troisième chapitre, pour comprendre les mécanismes de la sonoporation, un deuxième système de cavitation contrôlé a été conçu dans le but de permettre une visualisation du milieu en cours d’insonification. Ce nouveau dispositif est adapté à un fonctionnement sous microscope photonique à transmission et à fluorescence. Des essais de transfection de siRNAs, sur les cellules en suspension (RL du lymphome folliculaire) et sur les cellules adhérentes (cancer du sein ; MDA-MB 231) ont permis de valider in vitro l’efficacité de ce système en atteignant un taux de 40 % de transfection pour ces deux types de cellules, avec un très faible taux de mortalité (< 10 %)

The aim of the present work, which is based on the study and the control of acoustic cavitation, is to develop an efficient sonoporation system to transfect the cells in suspension and the adherent cells. The manuscript is composed of three chapters. The first one takes a glance on the state of art of different physical techniques used in cells transfection, and more precisely on sonoporation. It has been shown that the principal mechanism of sonoporation is closely linked to acoustic cavitation. Thus, a control of this random phenomenon is important to increase the rate of transfection while keeping strong cell viability. In the second chapter, a regulated cavitation generator based on an acoustic index was studied. This index is based on the measure of broad band noise emitted during the implosion of the cavitation bubbles. The advantage of such a system is: a control in real time of the level cavitation during sonication, leading to a better reproducibility and stability of the cavitation level, especially for the moderate intensities. In the third chapter, in order further study the sonoporation mechanisms, a second regulated…

Advisors/Committee Members: Béra, Jean-Christophe (thesis director), Mestas, Jean-Louis (thesis director).

Subjects/Keywords: Acoustique; Cavitation; Ultrasons; Sonoporation; Seuil de cavitation; Régulation; Transfection; Acoustics; Cavitation; Ultrasound; Sonoporation; Cavitation threshold; Regulation; Transfection; 620.2

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Sabraoui, A. (2012). Régulation de la cavitation acoustique appliquée à la transfection cellulaire : Feedback loop process to control acoustic cavitation : application to cell transfection. (Doctoral Dissertation). Université Claude Bernard – Lyon I. Retrieved from http://www.theses.fr/2012LYO10007

Chicago Manual of Style (16^th Edition):

Sabraoui, Abbas. “Régulation de la cavitation acoustique appliquée à la transfection cellulaire : Feedback loop process to control acoustic cavitation : application to cell transfection.” 2012. Doctoral Dissertation, Université Claude Bernard – Lyon I. Accessed January 23, 2021. http://www.theses.fr/2012LYO10007.

MLA Handbook (7^th Edition):

Sabraoui, Abbas. “Régulation de la cavitation acoustique appliquée à la transfection cellulaire : Feedback loop process to control acoustic cavitation : application to cell transfection.” 2012. Web. 23 Jan 2021.

Vancouver:

Sabraoui A. Régulation de la cavitation acoustique appliquée à la transfection cellulaire : Feedback loop process to control acoustic cavitation : application to cell transfection. [Internet] [Doctoral dissertation]. Université Claude Bernard – Lyon I; 2012. [cited 2021 Jan 23]. Available from: http://www.theses.fr/2012LYO10007.

Council of Science Editors:

Sabraoui A. Régulation de la cavitation acoustique appliquée à la transfection cellulaire : Feedback loop process to control acoustic cavitation : application to cell transfection. [Doctoral Dissertation]. Université Claude Bernard – Lyon I; 2012. Available from: http://www.theses.fr/2012LYO10007

Brno University of Technology

22. Bílek, Ondřej. Využití magnetických nanočástic pro transfekci plasmidu do HEK293 buněk: Magnetic Nanoparticles for Targeted Delivery System of Plasmid into HEK293 cells.

Degree: 2019, Brno University of Technology

URL: http://hdl.handle.net/11012/40709

► This bachelor’s thesis deals with using of magnetic nanoparticles, SPIO nanoparticles and nanoparticles of commercial product MATra for transfection of ASAP plasmid into HEK293 cells… (more)

Subjects/Keywords: Magnetické nanočástice; SPIO nanočástice; MATra; transfekce; DNA; účinnost transfekce; Magnetic nanoparticles; SPIO nanoparticles; MATra; transfection; DNA; transfection efficiency

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Bílek, O. (2019). Využití magnetických nanočástic pro transfekci plasmidu do HEK293 buněk: Magnetic Nanoparticles for Targeted Delivery System of Plasmid into HEK293 cells. (Thesis). Brno University of Technology. Retrieved from http://hdl.handle.net/11012/40709

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Bílek, Ondřej. “Využití magnetických nanočástic pro transfekci plasmidu do HEK293 buněk: Magnetic Nanoparticles for Targeted Delivery System of Plasmid into HEK293 cells.” 2019. Thesis, Brno University of Technology. Accessed January 23, 2021. http://hdl.handle.net/11012/40709.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Bílek, Ondřej. “Využití magnetických nanočástic pro transfekci plasmidu do HEK293 buněk: Magnetic Nanoparticles for Targeted Delivery System of Plasmid into HEK293 cells.” 2019. Web. 23 Jan 2021.

Vancouver:

Bílek O. Využití magnetických nanočástic pro transfekci plasmidu do HEK293 buněk: Magnetic Nanoparticles for Targeted Delivery System of Plasmid into HEK293 cells. [Internet] [Thesis]. Brno University of Technology; 2019. [cited 2021 Jan 23]. Available from: http://hdl.handle.net/11012/40709.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Bílek O. Využití magnetických nanočástic pro transfekci plasmidu do HEK293 buněk: Magnetic Nanoparticles for Targeted Delivery System of Plasmid into HEK293 cells. [Thesis]. Brno University of Technology; 2019. Available from: http://hdl.handle.net/11012/40709

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

23. Raisin, Sophie. Supports biomimétiques actifs pour la différenciation de cellules souches mésenchymateuses : application à la régénération du cartilage : Active biomimetic supports for mesenchymal stem cells : application to cartilage regeneration.

Degree: Docteur es, Chimie et physico-chimie des matériaux, 2016, Montpellier

URL: http://www.theses.fr/2016MONTT232

► La conception de biomatériaux actifs est actuellement encouragée par le manque de thérapies régénératives efficaces pour des tissus endommagés présentant une faible capacité d’autoréparation. Les… (more)

▼ La conception de biomatériaux actifs est actuellement encouragée par le manque de thérapies régénératives efficaces pour des tissus endommagés présentant une faible capacité d’autoréparation. Les progrès récents concernant les techniques de préparation de matériaux structurés (électrospinning, microfluidique) ainsi que la découverte du fort potentiel régénératif des cellules souches ont suscité un regain d’intérêt pour des projets collaboratifs à l’interface entre biologie et sciences des matériaux. Une approche prometteuse de régénération tissulaire repose donc sur la combinaison de cellules souches et de biomatériaux implantables. Des biomatériaux innovants, injectables et servants à la fois de support aux cellules et de réservoir de molécules actives telles que des protéines ou des agents de thérapie génique (Matrice Génétiquement Activée) ont été développés. Se plaçant plus particulièrement dans le contexte de l’ingénierie du cartilage, ce travail a pour objectif de développer une stratégie complémentaire concernant l’orientation de la différenciation de cellules souches mésenchymateuses (CSM) grâce au mécanisme d’interférence ARN.La principale difficulté rencontrée lors de l’utilisation d’acides nucléiques pour induire la différenciation des CSM reste leur faible capacité à traverser les membranes cellulaires, due à leur nature hydrophile et leur charge négative. De plus, les acides nucléiques sont dégradés très facilement par les nucléases extracellulaires, ce qui rend nécessaire l’utilisation d’un vecteur. Les vecteurs non-viraux sont d’excellents candidats pour des applications in vivo en raison de leur faible coût de production et leur faible immunogénicité. Toutefois, la plupart des systèmes de vectorisation trouvés dans la littérature présentent un manque de reproductibilité associé à une cytotoxicité vis-à-vis des cellules primaires. Nous souhaitions donc développer un système de transfection synthétique à la fois efficace et biocompatible. Pour cela, nous nous sommes basés sur les résultats encourageants concernant l’utilisation des micelles de complexes polyioniques (PIC) pour la transfection des cellules dendritiques. Ces micelles sont formées par complexation de deux polyélectrolytes : un copolymère à blocs double-hydrophiles (CBDH) avec un bloc anionique et un homopolymère cationique. Dans ce travail, nous avons évalué le polyoxyde d’éthylène – b – polyacide méthacrylique en tant que CBDH et la poly-L-lysine ou le polyéthylènimine en tant que polycation. L’influence des caractéristiques des composantes (asymétrie du CBDH, nature du polycation, taille des blocs, ratio de charges…) sur les propriétés physico-chimiques des micelles formées (taille, charge de surface) a d’abord été étudiée. Puis, la possibilité de complexation d’un siRNA au sein des micelles ainsi que leur stabilité en conditions physiologiques ont été évaluées. La formulation des micelles a été conçue pour permettre une dissociation des objets à un pH comparable à celui des endosomes ; ceci a été vérifié par diffusion dynamique de la… Advisors/Committee Members: Belamie, Emmanuel (thesis director).

Subjects/Keywords: Matériaux biofonctionnels; Cellules souches; Nanoparticules; Transfection; SiRNA; Matériaux biomimétiques; Biofunctional materials; Stem cells; Nanoparticles; Transfection; SiRNA; Biomimetic materials

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Raisin, S. (2016). Supports biomimétiques actifs pour la différenciation de cellules souches mésenchymateuses : application à la régénération du cartilage : Active biomimetic supports for mesenchymal stem cells : application to cartilage regeneration. (Doctoral Dissertation). Montpellier. Retrieved from http://www.theses.fr/2016MONTT232

Chicago Manual of Style (16^th Edition):

Raisin, Sophie. “Supports biomimétiques actifs pour la différenciation de cellules souches mésenchymateuses : application à la régénération du cartilage : Active biomimetic supports for mesenchymal stem cells : application to cartilage regeneration.” 2016. Doctoral Dissertation, Montpellier. Accessed January 23, 2021. http://www.theses.fr/2016MONTT232.

MLA Handbook (7^th Edition):

Raisin, Sophie. “Supports biomimétiques actifs pour la différenciation de cellules souches mésenchymateuses : application à la régénération du cartilage : Active biomimetic supports for mesenchymal stem cells : application to cartilage regeneration.” 2016. Web. 23 Jan 2021.

Vancouver:

Raisin S. Supports biomimétiques actifs pour la différenciation de cellules souches mésenchymateuses : application à la régénération du cartilage : Active biomimetic supports for mesenchymal stem cells : application to cartilage regeneration. [Internet] [Doctoral dissertation]. Montpellier; 2016. [cited 2021 Jan 23]. Available from: http://www.theses.fr/2016MONTT232.

Council of Science Editors:

Raisin S. Supports biomimétiques actifs pour la différenciation de cellules souches mésenchymateuses : application à la régénération du cartilage : Active biomimetic supports for mesenchymal stem cells : application to cartilage regeneration. [Doctoral Dissertation]. Montpellier; 2016. Available from: http://www.theses.fr/2016MONTT232

NSYSU

24. Wu, Bing-Ru. A unique neuronal plasticity-related gene of tilapia, Oreochromis mossambicus.

Degree: Master, Marine Biotechnology and Resources, 2013, NSYSU

URL: http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0805113-172845

► Oreochromis mossambicus is as a model organisms for researching molecular neural development biology in our research laboratory. A unique gene chimaerin (CHN1) out of the… (more)

Subjects/Keywords: Neuro-2a cell; neural development-related gene; Oreochromis mossambicus; transfection; protein

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Wu, B. (2013). A unique neuronal plasticity-related gene of tilapia, Oreochromis mossambicus. (Thesis). NSYSU. Retrieved from http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0805113-172845

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Wu, Bing-Ru. “A unique neuronal plasticity-related gene of tilapia, Oreochromis mossambicus.” 2013. Thesis, NSYSU. Accessed January 23, 2021. http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0805113-172845.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Wu, Bing-Ru. “A unique neuronal plasticity-related gene of tilapia, Oreochromis mossambicus.” 2013. Web. 23 Jan 2021.

Vancouver:

Wu B. A unique neuronal plasticity-related gene of tilapia, Oreochromis mossambicus. [Internet] [Thesis]. NSYSU; 2013. [cited 2021 Jan 23]. Available from: http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0805113-172845.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Wu B. A unique neuronal plasticity-related gene of tilapia, Oreochromis mossambicus. [Thesis]. NSYSU; 2013. Available from: http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0805113-172845

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

NSYSU

25. Hung, Shih-chieh. A study of DLX6 gene in tilapia train, Oreochromis mossambicus.

Degree: Master, Marine Biotechnology and Resources, 2013, NSYSU

URL: http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0811113-162141

► The unique gene, Distal-less homeobox (DLX6) was identified in the expressed sequence taq (EST) derived from the developing tilapia brain in our lab. The cDNA… (more)

Subjects/Keywords: Tilapia; differentiation; Neuro-2a; laser confocal microscopy; transfection; proliferation

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Hung, S. (2013). A study of DLX6 gene in tilapia train, Oreochromis mossambicus. (Thesis). NSYSU. Retrieved from http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0811113-162141

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Hung, Shih-chieh. “A study of DLX6 gene in tilapia train, Oreochromis mossambicus.” 2013. Thesis, NSYSU. Accessed January 23, 2021. http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0811113-162141.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Hung, Shih-chieh. “A study of DLX6 gene in tilapia train, Oreochromis mossambicus.” 2013. Web. 23 Jan 2021.

Vancouver:

Hung S. A study of DLX6 gene in tilapia train, Oreochromis mossambicus. [Internet] [Thesis]. NSYSU; 2013. [cited 2021 Jan 23]. Available from: http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0811113-162141.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Hung S. A study of DLX6 gene in tilapia train, Oreochromis mossambicus. [Thesis]. NSYSU; 2013. Available from: http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0811113-162141

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

NSYSU

26. Cheng, Pei-Hsin. Expression and characterization of truncated HGF in human breast cancer cell.

Degree: Master, Biological Sciences, 2007, NSYSU

URL: http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0722107-203940

► Hepatocyte growth factor (HGF) is a multifunctional mitogen, stimulating cell proliferation, motility, angiogenesis and morphogenesis via activating its receptor, c-Met tyrosine kinase. Overexpression of HGF… (more)

Subjects/Keywords: mitogen; transfection; signaling; proliferation; migration

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Cheng, P. (2007). Expression and characterization of truncated HGF in human breast cancer cell. (Thesis). NSYSU. Retrieved from http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0722107-203940

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Cheng, Pei-Hsin. “Expression and characterization of truncated HGF in human breast cancer cell.” 2007. Thesis, NSYSU. Accessed January 23, 2021. http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0722107-203940.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Cheng, Pei-Hsin. “Expression and characterization of truncated HGF in human breast cancer cell.” 2007. Web. 23 Jan 2021.

Vancouver:

Cheng P. Expression and characterization of truncated HGF in human breast cancer cell. [Internet] [Thesis]. NSYSU; 2007. [cited 2021 Jan 23]. Available from: http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0722107-203940.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Cheng P. Expression and characterization of truncated HGF in human breast cancer cell. [Thesis]. NSYSU; 2007. Available from: http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0722107-203940

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

NSYSU

27. Ciou, Ting-Jia. The study of unique functional gene cloned from tilapia, Oreochromis mossambicus.

Degree: Master, Marine Biotechnology and Resources, 2012, NSYSU

URL: http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0912112-132120

► The unique gene, pleiotrophin (ptn) was identified in the expressed sequence taq (EST) derived from the developing tilapia brain in our lab. The cDNA full… (more)

Subjects/Keywords: Tilapia; Neuro-2a; transfection; proliferation; differentiation; laser confocal microscopy

Record Details Similar Records Cite « Share

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Ciou, T. (2012). The study of unique functional gene cloned from tilapia, Oreochromis mossambicus. (Thesis). NSYSU. Retrieved from http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0912112-132120

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Ciou, Ting-Jia. “The study of unique functional gene cloned from tilapia, Oreochromis mossambicus.” 2012. Thesis, NSYSU. Accessed January 23, 2021. http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0912112-132120.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Ciou, Ting-Jia. “The study of unique functional gene cloned from tilapia, Oreochromis mossambicus.” 2012. Web. 23 Jan 2021.

Vancouver:

Ciou T. The study of unique functional gene cloned from tilapia, Oreochromis mossambicus. [Internet] [Thesis]. NSYSU; 2012. [cited 2021 Jan 23]. Available from: http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0912112-132120.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Ciou T. The study of unique functional gene cloned from tilapia, Oreochromis mossambicus. [Thesis]. NSYSU; 2012. Available from: http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0912112-132120

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Universidade do Rio Grande do Sul

28. Lopes, Eliana Franco. Utilização de lipídio catiônico para neutralização da carga de DNA e aplicação do complexo na imunização de camundongos.

Degree: 2008, Universidade do Rio Grande do Sul

URL: http://hdl.handle.net/10183/13952

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Embora a imunização gênica apresente vantagens sobre as vacinas convencionais, nem sempre a produção de anticorpos e a conseqüente proteção conferida pelo sistema imune contra… (more)

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Embora a imunização gênica apresente vantagens sobre as vacinas convencionais, nem sempre a produção de anticorpos e a conseqüente proteção conferida pelo sistema imune contra o antígeno de interesse são satisfatórias. As causas da baixa eficácia da imunização gênica, nestes casos, ainda são pouco conhecidas, porém um dos pontos mais relevantes em todo o processo é a eficiência da transfecção do DNA. O sucesso desta metodologia depende do estabelecimento de um sistema de entrega de gene eficiente e seguro. De acordo com a teoria proposta de Kuhn et al. (1999), baixas concentrações de moléculas anfifílicas podem ser usadas para neutralizar a carga do DNA, sem a formação de lipossomos. Este trabalho teve como objetivo avaliar o uso do lipídio catiônico dimetildioctadecil brometo de amônio (DDAB) como neutralizador da carga do DNA para a imunização de camundongos. Para a realização dos experimentos, um plasmídeo expressando a proteína do capsídeo (CA) do vírus da artrite encefalite caprina (CAEV), sob o comando do promotor citomegalovírus humano (CMV), foi utilizado nas imunizações. Os camundongos foram divididos em cinco grupos e receberam, via subcutânea, três inoculações de 10 μg de DNA linear (grupo 1), DNA linear mínimo (grupo 2), DNA circular (grupo 3) associados a 2,7 μg de DDAB ou DNA circular nu (grupo 4). O grupo controle foi inoculado com 2,7 μg de DDAB em solução fisiológica. Os camundongos imunizados com o plasmídeo na forma linear associado ao DDAB apresentaram maiores títulos de anticorpos CA que os inoculados com a forma circular ou linear mínima. Os dados sugerem que o lipídio catiônico DDAB tem potencial de uso como veículo de transfecção gênica em animais.

Genetic immunization has several advantages over conventional vaccines. However, antibody production and protection against the antigen of interest are often not satisfactory. In these cases, the causes of the low effectiveness of the genetic immunization are still poorly understood. One of the most relevant points in the immunization process is the DNA delivery. A successful DNA vaccine depends on the establishment of an efficient and safe gene delivery system. In accordance with Kuhn et al. (1999), low concentrations of amphiphilic molecules can be used to neutralize the DNA charge without the formation of liposomes. The aim of this study was to examine the effect of cationic lipid dimethildioctadecyl-ammonium bromide (DDAB) as a charge neutralizer for DNA for the immunization in mice. A plasmid expressing the capsid protein (CA) of caprine arthritis encephalitis virus (CAEV) under the control of the human cytomegalovirus immediate early promoter (hCMVie1) was used in the immunization. Mice were grouped and immunized with three injections of either 10 μg of linear DNA (group 1), minimal linear DNA (group 2) or circular DNA (group 3) associated with 2,7 μg of DDAB or naked circular DNA (group 4). The control group received 2,7 μg of DDAB diluted in physiologic solution. Mice immunized with DNA in linear form associated with DDAB developed…

Advisors/Committee Members: Ravazzolo, Ana Paula.

Subjects/Keywords: Imunologia veterinaria : Camundongos; Transfection; DNA; Genetic immunization; Minimal linear DNA; DDAB

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Lopes, E. F. (2008). Utilização de lipídio catiônico para neutralização da carga de DNA e aplicação do complexo na imunização de camundongos. (Thesis). Universidade do Rio Grande do Sul. Retrieved from http://hdl.handle.net/10183/13952

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Lopes, Eliana Franco. “Utilização de lipídio catiônico para neutralização da carga de DNA e aplicação do complexo na imunização de camundongos.” 2008. Thesis, Universidade do Rio Grande do Sul. Accessed January 23, 2021. http://hdl.handle.net/10183/13952.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Lopes, Eliana Franco. “Utilização de lipídio catiônico para neutralização da carga de DNA e aplicação do complexo na imunização de camundongos.” 2008. Web. 23 Jan 2021.

Vancouver:

Lopes EF. Utilização de lipídio catiônico para neutralização da carga de DNA e aplicação do complexo na imunização de camundongos. [Internet] [Thesis]. Universidade do Rio Grande do Sul; 2008. [cited 2021 Jan 23]. Available from: http://hdl.handle.net/10183/13952.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Lopes EF. Utilização de lipídio catiônico para neutralização da carga de DNA e aplicação do complexo na imunização de camundongos. [Thesis]. Universidade do Rio Grande do Sul; 2008. Available from: http://hdl.handle.net/10183/13952

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Universiteit Utrecht

29. Wolf, H.K. de. Cationic polymers for nuclaic acid delivery to tumors.

Degree: 2007, Universiteit Utrecht

URL: http://dspace.library.uu.nl:8080/handle/1874/24085

► In the field of cancer gene therapy, the use of gene carrier systems is considered indispensable. Cationic polymers are able to effectively condense plasmid DNA… (more)

▼ In the field of cancer gene therapy, the use of gene carrier systems is considered indispensable. Cationic polymers are able to effectively condense plasmid DNA to nano-sized particles, further referred to as polyplexes. Compared to free DNA, polyplexes have shown improved nuclease-resistance, a more efficient uptake by the target cells, and enhanced transfection activity. However, clinical introduction of polyplex-systems is hampered by concerns regarding toxicity and the extent and duration of gene expression mediated by the systems. Biodegradable cationic polymers show promise in non-viral gene delivery as they are likely to limit the long-term toxicity of polyplexes. Additionally, biodegradable polymers might also positively affect the gene expression profile mediated by polyplexes. So far, polyplexes based on biodegradable polymers have demonstrated low cytotoxicity and considerable in vitro transfection activity. Few studies reported on the in vivo transfection and toxicity characteristics of biodegradable polymers for gene delivery. In this thesis different biodegradable polymers were investigated for their in vivo tumor transfection properties in mice, after local as well as systemic administration. Different intravenously administered non-degradable cationic carriers were assessed for their capability to deliver nucleic acids to a distant tumor site. In addition, further optimization was explored by addressing the effect of polymer molecular weight and the bacterial motifs present in plasmid DNA on the tumor transfection activity of polyplexes. The in vivo evaluation of biodegradable polymers in this study has led to the identification of two gene delivery systems with considerable in vivo tumor transfection activities, i.e. p(DMAEA)-ppz and pHPMA-DMAE. Interestingly, the i.v. administration of p(DMAEA)-ppz yielded a tumor selective gene expression. The benefits of several advanced non-degradable carrier systems to deliver nucleic acids to a distant tumor site could not be attributed to benefits relevant during the transport phase. Rather, the benefits might be attributed to carrier-induced changes in the intratumoral processing of the nucleic acids. Finally, major improvements in the in vivo transfection efficiency/toxicity ratio of polyplex systems could be established by controlling the molecular weight of the polymeric vehicle and by the replacement of CpG motifs within the plasmid of the polyplexes.

Subjects/Keywords: Farmacie; polymer; DNA; gene delivery; transfection; tumor; biodistribution; polyplexes

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Wolf, H. K. d. (2007). Cationic polymers for nuclaic acid delivery to tumors. (Doctoral Dissertation). Universiteit Utrecht. Retrieved from http://dspace.library.uu.nl:8080/handle/1874/24085

Chicago Manual of Style (16^th Edition):

Wolf, H K de. “Cationic polymers for nuclaic acid delivery to tumors.” 2007. Doctoral Dissertation, Universiteit Utrecht. Accessed January 23, 2021. http://dspace.library.uu.nl:8080/handle/1874/24085.

MLA Handbook (7^th Edition):

Wolf, H K de. “Cationic polymers for nuclaic acid delivery to tumors.” 2007. Web. 23 Jan 2021.

Vancouver:

Wolf HKd. Cationic polymers for nuclaic acid delivery to tumors. [Internet] [Doctoral dissertation]. Universiteit Utrecht; 2007. [cited 2021 Jan 23]. Available from: http://dspace.library.uu.nl:8080/handle/1874/24085.

Council of Science Editors:

Wolf HKd. Cationic polymers for nuclaic acid delivery to tumors. [Doctoral Dissertation]. Universiteit Utrecht; 2007. Available from: http://dspace.library.uu.nl:8080/handle/1874/24085

University of California – Irvine

30. Shekaramiz, Elaheh. Integrated Electrowetting Nanoinjector/Aspirator for Single Cell Studies.

Degree: Biomedical Engineering, 2017, University of California – Irvine

URL: http://www.escholarship.org/uc/item/88g9c7zk

► Single cell transfection and analysis techniques are crucial for comprehending the heterogeneity that exists between cells. Many techniques have been introduced and evolved in recent… (more)

Subjects/Keywords: Engineering; Biology; Biophysics; Electrowetting; Nanoaspirator; Nanoinjector; Protein Analysis; Single Cell; Transfection

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Shekaramiz, E. (2017). Integrated Electrowetting Nanoinjector/Aspirator for Single Cell Studies. (Thesis). University of California – Irvine. Retrieved from http://www.escholarship.org/uc/item/88g9c7zk

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Shekaramiz, Elaheh. “Integrated Electrowetting Nanoinjector/Aspirator for Single Cell Studies.” 2017. Thesis, University of California – Irvine. Accessed January 23, 2021. http://www.escholarship.org/uc/item/88g9c7zk.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Shekaramiz, Elaheh. “Integrated Electrowetting Nanoinjector/Aspirator for Single Cell Studies.” 2017. Web. 23 Jan 2021.

Vancouver:

Shekaramiz E. Integrated Electrowetting Nanoinjector/Aspirator for Single Cell Studies. [Internet] [Thesis]. University of California – Irvine; 2017. [cited 2021 Jan 23]. Available from: http://www.escholarship.org/uc/item/88g9c7zk.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Shekaramiz E. Integrated Electrowetting Nanoinjector/Aspirator for Single Cell Studies. [Thesis]. University of California – Irvine; 2017. Available from: http://www.escholarship.org/uc/item/88g9c7zk

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Open Access Theses and Dissertations