subject:(transcription factors neuroglia polymerase chain reaction DNA microarrays in situ hybridization)

Freie Universität Berlin

1. Krämer, Nadine. Developmental molecular factors of the dentate gyrus in mice.

Degree: 2008, Freie Universität Berlin

URL: http://dx.doi.org/10.17169/refubium-8178

► The dentate gyrus provides a good model for diverse areas of research concerning the cortical development, especially neurogenesis, neuronal migration, regionalization, axonal guidance and neuronal… (more)

▼ The dentate gyrus provides a good model for diverse areas of research concerning the cortical development, especially neurogenesis, neuronal migration, regionalization, axonal guidance and neuronal plasticity. Furthermore, the dentate gyrus has the special ability to produce functional neurons out of neuronal precursors the whole life long. Therefore it is an interesting object of research for potential neuronal processes of regeneration in the cortex. The aim of this study was to identify molecular factors of dentate gyrus development and to analyse their functions. The subject of the experiments was a comparison of expression patterns from Emx2-/- mutant versus wildtype mouse, because the transcription factor Emx2 plays a key role in hippocampal development. Thus, mice deficient in this factor show very specific developmental alterations in the hippocampus, which leads to the absence of a morphologically visible dentate gyrus in these Emx2-/- mutants. First some known genes, whose functions had already been described in literature extensively, were analysed during development by in situ hybridisation. Thereby investigating the different functional circuits like proliferation, differentiation and migration as well as the structural alterations of the dentate gyrus and the adjacent hippocampal fissure and marginal zone were of particular interest. Furthermore some candidate genes, for which a putative relation to Emx2 had not been described previously, were identified by screening microarrays. The results show that, in the Emx2-/- mutant dentate gyrus, the proneural cascade of bHLH genes is present, but abnormally confined to the germinal layer, the neuronal and glial migrating precursors are nearly absent, the glial scaffold is defective, the few produced astrocytes and granule cells are not able to migrate to their proper destination and in addition the granule cells are deficiently differentiated. The glial scaffolding is also lacking in the Emx2-/- mutant developing hippocampal fissure. The inner marginal zone (IMZ) seems to be developed properly, in contrast the outer marginal zone (OMZ) as well as the dentate marginal zone (DMZ) is atrophic. Most of the Reln-expressing cells that should occupy the OMZ and DMZ are missing, except a specific subpopulation, characterized by using additional markers, probably born in the hem and for which Emx2 is not essentially required. The microarray analysis reveals a group of genes whose expression is reduced in the Emx2-/- hippocampus. For most of them a putative relation to Emx2 pathways had not been recognized previously. Some candidates are development genes or are involved in functional maturation and show expression in the hippocampal plate and/or developing dentate gyrus. A second group of candidates labels neuronal, glial or vascular structures in the OMZ and likely represent markers for cell populations specifically absent in the mutant. The lack of these genes points at different pathways and processes altered in the Emx2-/-mutant, particularly the Notch and… Advisors/Committee Members: n (gender), Univ.-Prof. Dr. H. Martens (firstReferee), Univ.-Prof. Dr. T. Skutella (furtherReferee), Univ.-Prof. Dr. H. Fink (furtherReferee).

Subjects/Keywords: Mice, hippocampus, embryonic development, neurons, migration, genes; Genes, Homeobox (MeSH); gene expression; Gene Expression Regulation, Developmental (MeSH); transcription factors, neuroglia, polymerase chain reaction, DNA microarrays, in-situ hybridization; 600 Technik, Medizin, angewandte Wissenschaften::630 Landwirtschaft

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Krämer, N. (2008). Developmental molecular factors of the dentate gyrus in mice. (Thesis). Freie Universität Berlin. Retrieved from http://dx.doi.org/10.17169/refubium-8178

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Krämer, Nadine. “Developmental molecular factors of the dentate gyrus in mice.” 2008. Thesis, Freie Universität Berlin. Accessed January 20, 2021. http://dx.doi.org/10.17169/refubium-8178.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Krämer, Nadine. “Developmental molecular factors of the dentate gyrus in mice.” 2008. Web. 20 Jan 2021.

Vancouver:

Krämer N. Developmental molecular factors of the dentate gyrus in mice. [Internet] [Thesis]. Freie Universität Berlin; 2008. [cited 2021 Jan 20]. Available from: http://dx.doi.org/10.17169/refubium-8178.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Krämer N. Developmental molecular factors of the dentate gyrus in mice. [Thesis]. Freie Universität Berlin; 2008. Available from: http://dx.doi.org/10.17169/refubium-8178

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Georgia

2. Tripathi, Niraj Kumar. Pathogenesis and treatment of Flavobacterium columnare-induced dermatitis in koi.

Degree: 2014, University of Georgia

URL: http://hdl.handle.net/10724/21193

► Columnaris disease is a bacterial infection of fish caused by the Gram-negative bacillus Flavobacterium columnare. An experimental model of this disease was developed to reproduce… (more)

▼ Columnaris disease is a bacterial infection of fish caused by the Gram-negative bacillus Flavobacterium columnare. An experimental model of this disease was developed to reproduce F. columnare-induced dermatitis in koi with high (80% to 100% ) mortality compared to uninfected controls (0% to 20% mortality). The protective mucus layer was wiped from the right side of the fish while leaving the left side intact. When fish were challenged with a virulent strain (strain 12/99) of F. columnare, cutaneous lesions consistently appeared on the right side of the body, but the left side of the body remained normal. This experimental model of columnaris disease differed from natural disease outbreaks in that cutaneous lesions were common while gill lesions were rare. The antibacterial properties of the surface mucus layer in preventing F. columnare infection was demonstrated by incubating cultures of log-phase growth bacteria with isolated mucus from healthy koi. F. columnare viability decreased as quantitated by manual counting of bacterial colonies on agar plates and by differentiation of viable and dead bacteria using propidium iodide staining and fluorescence microscopy. F. columnare in cytologic and histologic specimens appeared as long, thin, (5-12 mm x 0.5 mm) bacilli. Cytology was the best technique to tentatively diagnose columnaris disease. F. columnare was more readily visualized by Giemsa, as opposed to hematoxylin and eosin, staining in histologic sections. Both polymerase chain reaction (PCR) and DNA in-situ hybridization (ISH) diagnostic assays were developed to specifically detect F. columnare nucleic acid in fresh biological samples and in formalin fixed, paraffin embedded tissues. These techniques demonstrated that F. columnare infection in koi was restricted to the skin, fins, and, rarely, gills; systemic infection did not occur. Hematologic and biochemical reference intervals were established to evaluate subsequent changes during experimental disease. Hematologic changes in F. columnare infected koi included a nonregenerative anemia and mild leukopenia with lymphopenia, neutrophilia, and monocytosis. Biochemical changes included significantly decreased sodium and chloride concentrations. Decreases in total serum protein and albumin concentrations were present but minimal. A new proprietary solution (Tricide-Neo), was evaluated as a treatment for columnaris disease. Following experimental challenge with F. columnare, Tricide-Neo reduced mortality by 40% to 50% if treatment was performed before deep skin ulcers developed.

Subjects/Keywords: Cyprinus carpio; carp; fish; koi; disease model; columnaris disease; Flavobacterium columnare; polymerase chain reaction; DNA in-situ hybridization; reference intervals; hematology; blood cell morphology; ultrastructure; cytochemistry

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Tripathi, N. K. (2014). Pathogenesis and treatment of Flavobacterium columnare-induced dermatitis in koi. (Thesis). University of Georgia. Retrieved from http://hdl.handle.net/10724/21193

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Tripathi, Niraj Kumar. “Pathogenesis and treatment of Flavobacterium columnare-induced dermatitis in koi.” 2014. Thesis, University of Georgia. Accessed January 20, 2021. http://hdl.handle.net/10724/21193.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Tripathi, Niraj Kumar. “Pathogenesis and treatment of Flavobacterium columnare-induced dermatitis in koi.” 2014. Web. 20 Jan 2021.

Vancouver:

Tripathi NK. Pathogenesis and treatment of Flavobacterium columnare-induced dermatitis in koi. [Internet] [Thesis]. University of Georgia; 2014. [cited 2021 Jan 20]. Available from: http://hdl.handle.net/10724/21193.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Tripathi NK. Pathogenesis and treatment of Flavobacterium columnare-induced dermatitis in koi. [Thesis]. University of Georgia; 2014. Available from: http://hdl.handle.net/10724/21193

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Georgia

3. Pennick, Kate Elizabeth. Development of an ELISA to determine Aleutian mink disease parvovirus (ADV) capsid protein antibody titers.

Degree: 2014, University of Georgia

URL: http://hdl.handle.net/10724/22755

► Aleutian mink disease parvovirus (ADV) is a naturally occurring, autonomous parvovirus that is capable of infecting some members of the Mustelidae family. Although this virus… (more)

Subjects/Keywords: Aleutian mink disease virus (ADV); parvovirus; ferret; enzyme-linked immunosorbent assay (ELISA); counterimmunoelectrophoresis (CIEP); polymerase chain reaction (PCR); DNA in situ hybridization

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Pennick, K. E. (2014). Development of an ELISA to determine Aleutian mink disease parvovirus (ADV) capsid protein antibody titers. (Thesis). University of Georgia. Retrieved from http://hdl.handle.net/10724/22755

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Pennick, Kate Elizabeth. “Development of an ELISA to determine Aleutian mink disease parvovirus (ADV) capsid protein antibody titers.” 2014. Thesis, University of Georgia. Accessed January 20, 2021. http://hdl.handle.net/10724/22755.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Pennick, Kate Elizabeth. “Development of an ELISA to determine Aleutian mink disease parvovirus (ADV) capsid protein antibody titers.” 2014. Web. 20 Jan 2021.

Vancouver:

Pennick KE. Development of an ELISA to determine Aleutian mink disease parvovirus (ADV) capsid protein antibody titers. [Internet] [Thesis]. University of Georgia; 2014. [cited 2021 Jan 20]. Available from: http://hdl.handle.net/10724/22755.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Pennick KE. Development of an ELISA to determine Aleutian mink disease parvovirus (ADV) capsid protein antibody titers. [Thesis]. University of Georgia; 2014. Available from: http://hdl.handle.net/10724/22755

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

4. Zanardo, Evelin Aline. Avaliação de métodos citogenômicos para diagnóstico de pacientes com malformações congênitas e atraso do desenvolvimento neuropsicomotor.

Degree: Mestrado, Pediatria, 2014, University of São Paulo

URL: http://www.teses.usp.br/teses/disponiveis/5/5141/tde-26022015-103225/ ;

► O genoma humano é composto por diversos tipos de variações estruturais, como por exemplo, as variações no número de cópias (CNVs), que, mesmo sendo muito… (more)

▼ O genoma humano é composto por diversos tipos de variações estruturais, como por exemplo, as variações no número de cópias (CNVs), que, mesmo sendo muito pequenas, podem gerar diversas alterações clínicas específicas, como as malformações congênitas e o atraso do desenvolvimento neuropsicomotor (MC/ADNPM). Para a detecção destas alterações existem diferentes técnicas citogenômicas dentre elas a FISH (Fluorescent in situ Hibridization) e a MLPA (Multiplex Ligation-dependent Probe Amplification), que investigam um número limitado de regiões do genoma, como as regiões envolvidas nas síndromes de microdeleções/microduplicações mais comuns e as regiões subteloméricas. Outros métodos como a cariotipagem clássica e o array genômico possibilitam uma análise completa do DNA em uma única reação, aumentando a taxa de detecção de desequilíbrios complexos. Alcançar um diagnóstico inequívoco é fundamental para entender a natureza da doença, fornecendo respostas sobre o prognóstico, sobre os riscos de recorrência e direcionando o paciente à terapia específica, o que pode minimizar o custo financeiro dessas doenças e até mesmo possibilitar a inclusão desses indivíduos na sociedade. O projeto teve como objetivo comparar a capacidade diagnóstica destas tecnologias (FISH, MLPA e array) para a elucidação etiológica de pacientes sindrômicos encaminhados para a unidade de genética. A casuística deste trabalho foi composta pela análise dos resultados das técnicas de FISH e/ou MLPA e array, utilizadas no diagnóstico de 78 pacientes com MC/ADNPM. Na técnica de FISH, empregada na análise genômica de 22 pacientes, foram utilizadas sondas locus específicas para as regiões das principais síndromes de microdeleção/microduplicação e para as regiões subteloméricas de cromossomos específicos. Por meio desta metodologia, foram identificados ~18,2% dos pacientes com diferentes alterações. Já a técnica de MLPA, utilizada no diagnóstico dos 78 pacientes, por meio dos kits para as principais síndromes de microdeleção/microduplicação e para as regiões subteloméricas, detectou ~34,6% de pacientes com diversas alterações. A técnica de array, realizada em todos os pacientes utilizando diferentes plataformas (Agilent, Affymetrix ou Illumina) apresentou uma taxa de ~42,3% de detecção de pacientes com pelo menos uma alteração patogênica e ~38,5% de pacientes com alterações benignas ou de significado clínico incerto. Ao avaliar as três técnicas concomitantemente foi verificada uma taxa de ~93,6% de concordância, apesar dos resultados não serem iguais em todos os casos e da técnica de MLPA não detectar ~66,2% das alterações em relação ao array. Os resultados obtidos corroboraram com dados da literatura, mas no geral a taxa de detecção foi superior às taxas descritas, o em que em parte pode ser devido ao critério de seleção dos pacientes, sugerindo fortemente que a hipótese clínica adequada é crucial para o sucesso da detecção de alteração. Embora o array seja a ferramenta mais eficiente para o diagnóstico de pacientes com malformações, seu uso como primeiro teste… Advisors/Committee Members: Kulikowski, Leslie Domenici.

Subjects/Keywords: Análise de sequência com séries de oligonucleotídeos; Anormalidades congênitas; Congenital malformations; Deficiências do desenvolvimento; Developmental delay; DNA copy number variations; Hibridização in situ fluorescente; In Situ hybridization fluorescence; Molecular diagnostic techniques; Multiplex polymerase chain reaction; Oligonucleotide array sequence analysis; Reação em cadeia da polimerase multiplex; Técnicas de diagnóstico molecular; Variações do número de cópias de DNA

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Zanardo, E. A. (2014). Avaliação de métodos citogenômicos para diagnóstico de pacientes com malformações congênitas e atraso do desenvolvimento neuropsicomotor. (Masters Thesis). University of São Paulo. Retrieved from http://www.teses.usp.br/teses/disponiveis/5/5141/tde-26022015-103225/ ;

Chicago Manual of Style (16^th Edition):

Zanardo, Evelin Aline. “Avaliação de métodos citogenômicos para diagnóstico de pacientes com malformações congênitas e atraso do desenvolvimento neuropsicomotor.” 2014. Masters Thesis, University of São Paulo. Accessed January 20, 2021. http://www.teses.usp.br/teses/disponiveis/5/5141/tde-26022015-103225/ ;.

MLA Handbook (7^th Edition):

Zanardo, Evelin Aline. “Avaliação de métodos citogenômicos para diagnóstico de pacientes com malformações congênitas e atraso do desenvolvimento neuropsicomotor.” 2014. Web. 20 Jan 2021.

Vancouver:

Zanardo EA. Avaliação de métodos citogenômicos para diagnóstico de pacientes com malformações congênitas e atraso do desenvolvimento neuropsicomotor. [Internet] [Masters thesis]. University of São Paulo; 2014. [cited 2021 Jan 20]. Available from: http://www.teses.usp.br/teses/disponiveis/5/5141/tde-26022015-103225/ ;.

Council of Science Editors:

Zanardo EA. Avaliação de métodos citogenômicos para diagnóstico de pacientes com malformações congênitas e atraso do desenvolvimento neuropsicomotor. [Masters Thesis]. University of São Paulo; 2014. Available from: http://www.teses.usp.br/teses/disponiveis/5/5141/tde-26022015-103225/ ;

NSYSU

5. Chien, Hua-yi. Use of In Situ Bioremediation to Treat Trichloroethylene-contaminated Groundwater.

Degree: PhD, Environmental Engineering, 2010, NSYSU

URL: http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0207110-212744

► Chlorinated aliphatic hydrocarbons (CAHs) include tetrachloroethene (PCE), trichloroethene (TCE), and others. The industrial solvent TCE is among the most ubiquitous chlorinated compounds found in groundwater… (more)

▼ Chlorinated aliphatic hydrocarbons (CAHs) include tetrachloroethene (PCE), trichloroethene (TCE), and others. The industrial solvent TCE is among the most ubiquitous chlorinated compounds found in groundwater pollution. TCE in environment can be removed by physical, chemical and biological procedures. Dehalorespiration is a biological pathway from which bacteria can derive energy from the reductive dechlorination of chlorinated ethenes using hydrogen or organic acids as electron donors and yielding chloride and ethene as degradation products. Dehalorespiration can be used to remediate chlorinated ethene contaminated aquifers if an appropriate aquifer ecosystem exists including populations of dechlorinating bacteria and companion organisms that contribute to the biogeochemical environment conducive to dehalorespiration activity. Enhanced in-situ aerobic or anaerobic bioremediation of chlorinated solvents is a cost-effective, expanding technology for the clean-up of chlorinated solvent-contaminated sites. The objective of this pilot-scale study was to apply an enhanced in situ bioremediation technology to remediate TCE-contaminated groundwater. Both aerobic and anaerobic remedial systems were evaluated at a TCE-spill site located in southern Taiwan. In the aerobic bioremediation zone, the effectiveness of air, nutrient, and sugarcane molasses injection to enhance the aerobic cometabolism on TCE degradation was evaluated. Results show that the decreases in TCE concentration were observed over 204 days operating period. Up to 73ï¼-99ï¼ of TCE removal efficiency was obtained in this treatment system. In the anaerobic test zone, the effectiveness of nutrient and sugarcane molasses injection to enhance the anaerobic dechlorination on TCE degradation was also evaluated. Results show that the decreases in TCE concentration were observed over a 193-day operating period. Up to 53ï¼-91ï¼ of TCE removal efficiency was obtained in this treatment system. Polymerase chain reaction was applied to analyze the gene variation in TCE-microbial degraders during the treatment process. Results from this study indicate that the aerobic TCE-degraders (typeâ methanotrophs and type â¡ methanotrophs) and the gene of degradation enzymes (toluene monooxygenase, toluene dioxygenase, particulate methane monooxygenase) were detected after the treatment process in the aerobic test zone. In the anaerobic treatment zone, Dehalococcoides (anaerobic TCE-degrader) and the gene of degradation enzyme (vcrA and tceA) were detected and a significant drop of TCE concentration was also observed. Based on 16S rDNA sequence analysis, samples of groundwater from aerobic/anaerobic bioremediation zone are close related to the genera of Dehalococcoides sp. MB, Dehalococcoides ethenogenes 195, Dehalococcoides sp. VS, Acidovorax sp., Alicycliphilus sp., Burkholderiales, Caulobacter sp., Caulobacter tuntrae, Caulobacter vibrioides, Comamonadaceae, Hydrogenophaga sp., Iron-reducing bacterium, Mitsuaria chitosanitabida, Rhodocyclacea, Pseudomonas sp., Rhodoferax… Advisors/Committee Members: Huang Wen-Yen (chair), Chen Ku-Fan (chair), Yu Kuang-Chung (chair), Lai Chin-Hsing (chair), Kao Chih-Ming (committee member), Tsai Li-Jyur (chair).

Subjects/Keywords: in situ bioremediation; polymerase chain reaction; groundwater pollution; trichloroethene

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Chien, H. (2010). Use of In Situ Bioremediation to Treat Trichloroethylene-contaminated Groundwater. (Doctoral Dissertation). NSYSU. Retrieved from http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0207110-212744

Chicago Manual of Style (16^th Edition):

Chien, Hua-yi. “Use of In Situ Bioremediation to Treat Trichloroethylene-contaminated Groundwater.” 2010. Doctoral Dissertation, NSYSU. Accessed January 20, 2021. http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0207110-212744.

MLA Handbook (7^th Edition):

Chien, Hua-yi. “Use of In Situ Bioremediation to Treat Trichloroethylene-contaminated Groundwater.” 2010. Web. 20 Jan 2021.

Vancouver:

Chien H. Use of In Situ Bioremediation to Treat Trichloroethylene-contaminated Groundwater. [Internet] [Doctoral dissertation]. NSYSU; 2010. [cited 2021 Jan 20]. Available from: http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0207110-212744.

Council of Science Editors:

Chien H. Use of In Situ Bioremediation to Treat Trichloroethylene-contaminated Groundwater. [Doctoral Dissertation]. NSYSU; 2010. Available from: http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0207110-212744

6. Amary, Maria Fernanda Carriel. Detecção da fusão SS18-SSX em material parafinado e comparação de métodos moleculares como ferramentas no diagnóstico do Sarcoma Sinovial.

Degree: PhD, Patologia, 2007, University of São Paulo

URL: http://www.teses.usp.br/teses/disponiveis/5/5144/tde-20082007-162629/ ;

►

O Sarcoma Sinovial revela consistentemente t(X;18) resultando em SS18- SSX1, SS18-SSX2 e raramente SS18-SSX4. Dos 328 casos incluídos neste estudo, Sarcoma Sinovial foi considerado a… (more)

▼

O Sarcoma Sinovial revela consistentemente t(X;18) resultando em SS18- SSX1, SS18-SSX2 e raramente SS18-SSX4. Dos 328 casos incluídos neste estudo, Sarcoma Sinovial foi considerado a primeira possibilidade diagnóstica ou um importante diagnóstico diferencial em 134 casos: destes, cDNA de qualidade foi obtido em 131. A fusão SS18-SSX foi identificada em 126 (96%) casos (74 SS18-SSX1, 52 SS18-SSX2) através de qRT-PCR e 120 (92%) por RT-PCR convencional. 101 casos no array de tecidos, analisados por FISH, revelaram que 87 (86%) mostraram rearranjo do SS18. Quatro casos positivos por RT-PCR mostraram perda de um sinal spectrum green e 15 casos revelaram cópias múltiplas de SS18: ambos os achados são potencialmente problemáticos na interpretação de resultados. Um dos 3 casos não analisados por RT-PCR por não ter gerado cDNA de qualidade, foi positivo por FISH. A fusão SS18-SSX1 foi demonstrada em 56 SS monofásicos e 18 SS bifásicos. SS18-SSX2 foi detectada em 41 monofásicos e 11 bifásicos. Áreas pouco diferenciadas foram identificadas em 44 casos (31%). Não houve correlação estatisticamente significante entre os subtipos bifásico, monofásico e o tipo de fusão. Cinco casos foram negativos através dos três métodos utilizados, três de localização pleural. Após correlação clínica, o diagnóstico de mesotelioma foi favorecido em um caso, tumor fibroso solitário em outro e o diagnóstico de sarcoma sem outras especificações no terceiro. A possibilidade do diagnóstico de TMBNP não pode ser excluída nos outros dois casos. Nós concluímos que os métodos moleculares são ferramentas auxiliares importantes para o diagnóstico de SS com 95% de sensibilidade e 100% de especificidade, mas os resultados devem ser interpretados à luz de características clínicas e dados imunohistoquímicos.

Synovial Sarcoma consistently harbors t(X;18) resulting in SS18-SSX1, SS18-SSX2 and rarely SS18-SSX4 fusion transcripts. Of 328 cases included in our study, synovial sarcoma was either the primary diagnosis or was very high in the differential diagnosis in 134 cases: of these, amplifiable cDNA was obtained from 131. SS18-SSX fusion products were found in 126 (96%) cases, (74 SS18-SSX1, 52 SS18-SSX2), using quantitative and 120 by conventional RT-PCR. 101 cases in a tissue microarray, analyzed by FISH, revealed that 87 (86%) showed SS18 rearrangement: 4 reverse transcriptase polymerase chain reaction positive cases, reported as negative for FISH, showed loss of one spectrum green signal, and 15 cases had multiple copies of the SS18 gene: both findings are potentially problematic when interpreting results. One of 3 cases, not analyzed by RT PCR due to poor quality RNA, was positive by FISH. SS18-SSX1 was present in 56 monophasic and 18 biphasic synovial sarcoma: SS18-SSX2 was detected in 41 monophasic and 11 biphasic synovial sarcoma. Poorly differentiated areas were identified in 44 cases (31%). There was no statistically significant association between biphasic, monophasic and fusion type. Five cases were negative for SS18 rearrangement by all methods, 3…

Advisors/Committee Members: Bernardi, Fabiola Del Carlo.

Subjects/Keywords: Biologia molecular; Fluorêscencia em hibridização in situ; In situ hybridization fluorescence; Molecular biology; Reação em cadeia da polimerase via transcriptase reversa; Reverse transcriptase polymerase chain reaction; Sarcoma; Sarcoma; Sarcoma sinovial; Sarcoma synovial

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Amary, M. F. C. (2007). Detecção da fusão SS18-SSX em material parafinado e comparação de métodos moleculares como ferramentas no diagnóstico do Sarcoma Sinovial. (Doctoral Dissertation). University of São Paulo. Retrieved from http://www.teses.usp.br/teses/disponiveis/5/5144/tde-20082007-162629/ ;

Chicago Manual of Style (16^th Edition):

Amary, Maria Fernanda Carriel. “Detecção da fusão SS18-SSX em material parafinado e comparação de métodos moleculares como ferramentas no diagnóstico do Sarcoma Sinovial.” 2007. Doctoral Dissertation, University of São Paulo. Accessed January 20, 2021. http://www.teses.usp.br/teses/disponiveis/5/5144/tde-20082007-162629/ ;.

MLA Handbook (7^th Edition):

Amary, Maria Fernanda Carriel. “Detecção da fusão SS18-SSX em material parafinado e comparação de métodos moleculares como ferramentas no diagnóstico do Sarcoma Sinovial.” 2007. Web. 20 Jan 2021.

Vancouver:

Amary MFC. Detecção da fusão SS18-SSX em material parafinado e comparação de métodos moleculares como ferramentas no diagnóstico do Sarcoma Sinovial. [Internet] [Doctoral dissertation]. University of São Paulo; 2007. [cited 2021 Jan 20]. Available from: http://www.teses.usp.br/teses/disponiveis/5/5144/tde-20082007-162629/ ;.

Council of Science Editors:

Amary MFC. Detecção da fusão SS18-SSX em material parafinado e comparação de métodos moleculares como ferramentas no diagnóstico do Sarcoma Sinovial. [Doctoral Dissertation]. University of São Paulo; 2007. Available from: http://www.teses.usp.br/teses/disponiveis/5/5144/tde-20082007-162629/ ;

7. Alexis Dourado Guedes. Determinação do Fenótipo Sexual em uma Criança com Mosaicismo 45,X/46,X,Idic(Yp): Importância da Proporção Relativa da Linhagem 45,X no Tecido Gonadal.

Degree: 2006, Universidade Federal de São Paulo

URL: http://www.bdtd.unifesp.br/tede//tde_busca/arquivo.php?codArquivo=76

►

Não tem.

We report here on a girl who, despite her 45,X/46,X,der(Y) karyotype, showed no signs of virilization or physical signs of the Ullrich-Turner syndrome… (more)

Subjects/Keywords: 1-Mosaicismo; 2-Diferenciação Sexual; 3-Isocromossomos; 4-Cromossomo Y; 5- Hibridação In Situ Fluorescente; 6- Reação de Cadeia de Polimerase.; ENDOCRINOLOGIA; mosaicism; sex differentiation; isochromosomes; Y chromosome; fluorescence in situ hybridization; polymerase chain reaction; Ullrich-Turner yndrome.

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Guedes, A. D. (2006). Determinação do Fenótipo Sexual em uma Criança com Mosaicismo 45,X/46,X,Idic(Yp): Importância da Proporção Relativa da Linhagem 45,X no Tecido Gonadal. (Thesis). Universidade Federal de São Paulo. Retrieved from http://www.bdtd.unifesp.br/tede//tde_busca/arquivo.php?codArquivo=76

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Guedes, Alexis Dourado. “Determinação do Fenótipo Sexual em uma Criança com Mosaicismo 45,X/46,X,Idic(Yp): Importância da Proporção Relativa da Linhagem 45,X no Tecido Gonadal.” 2006. Thesis, Universidade Federal de São Paulo. Accessed January 20, 2021. http://www.bdtd.unifesp.br/tede//tde_busca/arquivo.php?codArquivo=76.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Guedes, Alexis Dourado. “Determinação do Fenótipo Sexual em uma Criança com Mosaicismo 45,X/46,X,Idic(Yp): Importância da Proporção Relativa da Linhagem 45,X no Tecido Gonadal.” 2006. Web. 20 Jan 2021.

Vancouver:

Guedes AD. Determinação do Fenótipo Sexual em uma Criança com Mosaicismo 45,X/46,X,Idic(Yp): Importância da Proporção Relativa da Linhagem 45,X no Tecido Gonadal. [Internet] [Thesis]. Universidade Federal de São Paulo; 2006. [cited 2021 Jan 20]. Available from: http://www.bdtd.unifesp.br/tede//tde_busca/arquivo.php?codArquivo=76.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Guedes AD. Determinação do Fenótipo Sexual em uma Criança com Mosaicismo 45,X/46,X,Idic(Yp): Importância da Proporção Relativa da Linhagem 45,X no Tecido Gonadal. [Thesis]. Universidade Federal de São Paulo; 2006. Available from: http://www.bdtd.unifesp.br/tede//tde_busca/arquivo.php?codArquivo=76

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Florida

8. Pagliai Lopez, Fernando A. Molecular Insights on Protein-Ligand Interaction in the MarR Family of Transcriptional Regulators.

Degree: PhD, Microbiology and Cell Science, 2015, University of Florida

URL: https://ufdc.ufl.edu/UFE0049507

► Gene expression in bacteria is mostly regulated by one-component transcriptional regulators. These proteins contain a DNA-binding site and an allosteric site for metabolite interaction. Transcriptional… (more)

▼ Gene expression in bacteria is mostly regulated by one-component transcriptional regulators. These proteins contain a DNA-binding site and an allosteric site for metabolite interaction. Transcriptional regulators are classified into protein families based on the similarity of the DNA-binding domain. However, this classification fails to integrate the role of effector molecules in the mechanism of gene regulation. In this dissertation, it was proposed to assign functional roles to transcriptional regulators based on the effector molecules that they bind. To accomplish this goal, the MarR-family of transcriptional regulators was used as a model due to the large variety of biological processes that they regulate and the wide spectrum of chemical scaffolds that they sense. An integrative approach, including a high-throughput screening of small molecules, structural modeling, genomic associations, biochemical characterizations, and in vivo validations, led to the identification of SAL1 ligand binding pocket as an essential feature in the regulatory activity of MarR homologs. The role of proteins with unknown function, regulated by a MarR homolog, such as LVIS_0552 and LVIS_0852, was elucidated using Lactobacillus brevis as a model. LVIS_0552 was identified as new transport protein involved in the efflux of novobiocin, whereas LVIS_0852 (named TstT) was identified as a new class of thiosulfate sulfurtransferase involved in the detoxification of cyanide. Also, a new role for a member of the MarR-family was discovered. Besides acting as a transcriptional repressor of tstT, the transcriptional regulator TstR increased the enzymatic activity of TstT, in a process favored by the presence of iron. These findings suggest that the SAL1 pocket could be targeted by small molecules to inhibit a MarR activator, such as in LdtR from the citrus pathogen Liberibacter asiaticus. Thus, competitive inhibitors of native ligand(s) that bind to the SAL1 pocket of MarR transcriptional activators are potential therapeutics in the control of pathogenesis. ( en ) Advisors/Committee Members: LORCA,GRACIELA L (committee chair), FOSTER,JAMIE S (committee member), GONZALEZ,CLAUDIO F (committee member), OSTROV,DAVID A (committee member).

Subjects/Keywords: Amino acids; Binding sites; Chemicals; Cyanides; DNA; Ligands; Molecules; Operon; Plasmids; Polymerase chain reaction; huanglongbing – lactobacillus – marr – transcription

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Pagliai Lopez, F. A. (2015). Molecular Insights on Protein-Ligand Interaction in the MarR Family of Transcriptional Regulators. (Doctoral Dissertation). University of Florida. Retrieved from https://ufdc.ufl.edu/UFE0049507

Chicago Manual of Style (16^th Edition):

Pagliai Lopez, Fernando A. “Molecular Insights on Protein-Ligand Interaction in the MarR Family of Transcriptional Regulators.” 2015. Doctoral Dissertation, University of Florida. Accessed January 20, 2021. https://ufdc.ufl.edu/UFE0049507.

MLA Handbook (7^th Edition):

Pagliai Lopez, Fernando A. “Molecular Insights on Protein-Ligand Interaction in the MarR Family of Transcriptional Regulators.” 2015. Web. 20 Jan 2021.

Vancouver:

Pagliai Lopez FA. Molecular Insights on Protein-Ligand Interaction in the MarR Family of Transcriptional Regulators. [Internet] [Doctoral dissertation]. University of Florida; 2015. [cited 2021 Jan 20]. Available from: https://ufdc.ufl.edu/UFE0049507.

Council of Science Editors:

Pagliai Lopez FA. Molecular Insights on Protein-Ligand Interaction in the MarR Family of Transcriptional Regulators. [Doctoral Dissertation]. University of Florida; 2015. Available from: https://ufdc.ufl.edu/UFE0049507

NSYSU

9. Wang, Yu-Chi. Expression of Immune-Related Genes in the Pacific White Shrimp, Litopenaeus vannamei and Their odulation by beta-glucan via Oral Administration.

Degree: PhD, Marine Biology, 2007, NSYSU

URL: http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0704107-153332

► The present study investigated the expression profiles of nine genes involved in immune defense of the Pacific white shrimp Litopenaeus vannamei and their responses to… (more)

▼ The present study investigated the expression profiles of nine genes involved in immune defense of the Pacific white shrimp Litopenaeus vannamei and their responses to oral administration of beta-1,3-glucan. The nine immune related genes were beta-glucan binding protein-high density lipoprotein (BGBP-HDL), lipopolysaccharide/beta-glucan binding protein (LGBP), hemocyanin, prophenoloxidase (proPO), transglutaminase (TGase), penaeidin-3 (PEN-3), crustin, cytosolic manganese superoxide dismutase (cMnSOD), and lysozyme. A series of experiments were carried out in the study including: (1) cDNA cloning and characterization of proPO and LGBP; (2) tissue mRNA expressions of the nine genes in adult shrimp; (3) expression and localization of the nine genes during larval and postlarval ontogenic development; (4) the effects of dietary beta-1,3-glucan on the expression of the nine genes. The cDNA cloning study showed that the proPO cDNA contains an open reading frame of 2061 bp and encodes a 686 amino-acid peptide. The protein sequence of the proPO has a similarity of 85% with those of Penaeus monodon and P. semisulcatus and has an identity of between 58 and 77% with other crustaceans. Northern blot analysis revealed that proPO was constitutively expressed mainly in hemocytes. Its transcripts were observed in hemocytes and many other tissues when detected with RT-PCR. The results of in situ hybridizations showed that the hemocytes that infiltrated in tissues were responsible for the positive signals. The LGBP cDNA contains an open reading frame of 1104 bp and encodes a 367 amino-acid protein with a 17 a. a. signal peptide. The protein sequence of the LGBP has a similarity of 97% with LGBP of L. stylirostris, >90% identity with BGBP of P. monodon and LGBP of Fenneropenaeus chinensis and has an identity of between 63 and 86% with other crustaceans. Northern blot analysis revealed that LGBP was constitutively expressed mainly in hepatopancreas. The results of in situ hybridizations showed that the hepatopancreatic F cells might be the major cell type for LGBP production. Using the complete cDNAs of proPO and LGBP and partial fragments of the other seven genes, their tissue expressions were analyzed by conventional RT-PCR, quantitative real time PCR (qPCR) and in situ hybridization. The results demonstrated that BGBP-HDL, LGBP and hemocyanin were mainly expressed in the hepatopancreas and their expressions levels were about 10 to 30% those of Advisors/Committee Members: Cheng-Fang Chang (chair), Ching-Ming Kuo (chair), Jiann-Chu Chen (chair), Houng-Yung Chen (committee member), Yen-Ling Song (chair), Winton Cheng (chair).

Subjects/Keywords: immune; Litopenaeus vannamei; gene expression; in situ hybridization; beta-glucan; ontogenesis; quantitative real-time polymerase chain reaction (qPCR)

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Wang, Y. (2007). Expression of Immune-Related Genes in the Pacific White Shrimp, Litopenaeus vannamei and Their odulation by beta-glucan via Oral Administration. (Doctoral Dissertation). NSYSU. Retrieved from http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0704107-153332

Chicago Manual of Style (16^th Edition):

Wang, Yu-Chi. “Expression of Immune-Related Genes in the Pacific White Shrimp, Litopenaeus vannamei and Their odulation by beta-glucan via Oral Administration.” 2007. Doctoral Dissertation, NSYSU. Accessed January 20, 2021. http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0704107-153332.

MLA Handbook (7^th Edition):

Wang, Yu-Chi. “Expression of Immune-Related Genes in the Pacific White Shrimp, Litopenaeus vannamei and Their odulation by beta-glucan via Oral Administration.” 2007. Web. 20 Jan 2021.

Vancouver:

Wang Y. Expression of Immune-Related Genes in the Pacific White Shrimp, Litopenaeus vannamei and Their odulation by beta-glucan via Oral Administration. [Internet] [Doctoral dissertation]. NSYSU; 2007. [cited 2021 Jan 20]. Available from: http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0704107-153332.

Council of Science Editors:

Wang Y. Expression of Immune-Related Genes in the Pacific White Shrimp, Litopenaeus vannamei and Their odulation by beta-glucan via Oral Administration. [Doctoral Dissertation]. NSYSU; 2007. Available from: http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0704107-153332

University of Florida

10. Gussow, Ann M. Characterization of a transgenic mouse expressing the HSV-1 latency associated transcript.

Degree: 2004, University of Florida

URL: https://ufdc.ufl.edu/AA00026430

Subjects/Keywords: Cells; DNA; Human herpesvirus 1; Infections; Introns; Neurons; Polymerase chain reaction; Promoter regions; RNA; Transgenes; Department of Molecular Genetics and Microbiology thesis Ph.D ( mesh ); Herpesvirus 1, Human – pathogenicity ( mesh ); In Situ Hybridization – methods ( mesh ); Mouse, Transgenic ( mesh ); Polymerase Chain Reaction – methods ( mesh ); Virus Latency ( mesh )

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Gussow, A. M. (2004). Characterization of a transgenic mouse expressing the HSV-1 latency associated transcript. (Thesis). University of Florida. Retrieved from https://ufdc.ufl.edu/AA00026430

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Gussow, Ann M. “Characterization of a transgenic mouse expressing the HSV-1 latency associated transcript.” 2004. Thesis, University of Florida. Accessed January 20, 2021. https://ufdc.ufl.edu/AA00026430.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Gussow, Ann M. “Characterization of a transgenic mouse expressing the HSV-1 latency associated transcript.” 2004. Web. 20 Jan 2021.

Vancouver:

Gussow AM. Characterization of a transgenic mouse expressing the HSV-1 latency associated transcript. [Internet] [Thesis]. University of Florida; 2004. [cited 2021 Jan 20]. Available from: https://ufdc.ufl.edu/AA00026430.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Gussow AM. Characterization of a transgenic mouse expressing the HSV-1 latency associated transcript. [Thesis]. University of Florida; 2004. Available from: https://ufdc.ufl.edu/AA00026430

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Lethbridge

11. Witharana, Wing Kar Li. Non-Boolean characterization of Homer1a intranuclear transcription foci .

Degree: 2011, University of Lethbridge

URL: http://hdl.handle.net/10133/3402

► Activity-induced immediate-early gene (IEG) transcription foci can be labelled with fluorescent probes, permitting high temporal and spatial resolution in mapping neuronal circuits. Previous quantification approaches… (more)

Subjects/Keywords: Scaffold proteins – Research; Neuroplasticity; Transcription factors; Neural circuitry; Hippocampus (Brain); Rats as laboratory animals; Fluorescence in situ hybridization; Dissertations, Academic

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Witharana, W. K. L. (2011). Non-Boolean characterization of Homer1a intranuclear transcription foci . (Thesis). University of Lethbridge. Retrieved from http://hdl.handle.net/10133/3402

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Witharana, Wing Kar Li. “Non-Boolean characterization of Homer1a intranuclear transcription foci .” 2011. Thesis, University of Lethbridge. Accessed January 20, 2021. http://hdl.handle.net/10133/3402.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Witharana, Wing Kar Li. “Non-Boolean characterization of Homer1a intranuclear transcription foci .” 2011. Web. 20 Jan 2021.

Vancouver:

Witharana WKL. Non-Boolean characterization of Homer1a intranuclear transcription foci . [Internet] [Thesis]. University of Lethbridge; 2011. [cited 2021 Jan 20]. Available from: http://hdl.handle.net/10133/3402.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Witharana WKL. Non-Boolean characterization of Homer1a intranuclear transcription foci . [Thesis]. University of Lethbridge; 2011. Available from: http://hdl.handle.net/10133/3402

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Tartu University

12. Kaplinski, Lauris. The application of oligonucleotide hybridization model for PCR and microarray optimization .

Degree: 2013, Tartu University

URL: http://hdl.handle.net/10062/32163

► Nukleiinhapped on orgaaniliste makromolekulide hulgas unikaalsed tänu oma võimele kodeerida, dekodeerida ja kanda üle digitaalset informatsiooni. See omadus on aluseks nende kasutamisele arenevates tehnoloogiavaldkondades, alates… (more)

▼ Nukleiinhapped on orgaaniliste makromolekulide hulgas unikaalsed tänu oma võimele kodeerida, dekodeerida ja kanda üle digitaalset informatsiooni. See omadus on aluseks nende kasutamisele arenevates tehnoloogiavaldkondades, alates kliinilisest diagnostikast kuni nanotehnoloogia ja informatsiooni talletamiseni. On aga oluline mõista, et digitaalse informatsiooni töötlemise ja säilitamise aluseks nukleiinhapetes on nende keemilised omadused. Tähtsaim nendest on hübridiseerumine - nukleiinhapete võime moodustada spontaanselt kaheahelaline heeliks kahe komplementaarse või osaliselt komplementaarse üheahelalise molekuli liitumisel. Nukleiinhapete hübridisatsiooni termodünaamika arvestamine võimaldab selle protsessi käitumist suure täpsusega modelleerida ja täiustada paljusid biotehnoloogilisi protsesse. Käesolevas väitekirjas on hübridisatsioonimudelit kasutatud multipleks-PCR-i ja detektsiooni mikrokiipide optimeerimiseks. Me töötasime välja ökonoomse algoritmi jaotamaks PCR praimeripaarid multipleksigruppidesse vastavalt nende omavahelisele sobivusele. Algoritm on realiseeritud nii iseseisva programmi kui veebirakendusena. Me uurisime multipleks PCR ebaõnnestumise põhjuseid ja näitasime, et suur arv mittespetsiifilisi seondumiskohti lähte DNA-l vähendab praimerite töötamise edukust. Need praimeripaarid, millel oli liiga suur arv mittespetsiifilisi seondumisi mitte ainult ei töötanud ise halvasti, vaid vähendasid ka teiste nendega koos amplifiseeritud praimeripaaride õnnestumise tõenäosust. Me töötasime välja arvutiprogrammi genereerimaks täieliku nimekirja kõigist võimalikest bakteriaalse tmRNA hübridiseerimisproovidest mis eristaksid omavahel kahte gruppi organisme. Proovide valideerimise käigus me näitasime, et valides hübridisatsioonienergia läviväärtuse suurema kui 4 kcl/mol on võimalik täielikult vältida valepositiivseid signaale. Me uurisime võimalust suurendada bakteriaalse RNA hübridiseerumiskiirust lisades lühikesi spetsiifilisi oligonukleotiide, mis hübridiseerudes lähtemolekulile ei lase selle sekundaarstruktuuril moodustuda. Seda meetodit kasutades tõusis hübridiseerumiskiirus temperatuuril 37C neli korda.; Nucleic acids are unique among all organic macromolecules by the ability to encode, decode and transmit digital information. This property is used in emergent technologies as diverse as medical diagnosis, nanoscale engineering and information storage. Still it is important to understand that the basis of this digital information processing are the chemical properties of nucleic acids, the most important being the spontaneous formation of double-stranded helix between complementary or semi-complementary single-stranded molecules, called hybridization. Taking into account the thermodynamic properties of nucleic acid hybridization allows researchers to model the process with great accuracy and thus improve many associated technologies. In current thesis the hybridization model is used to optimize multiplex PCR and microarray hybridization. We developed an efficient algorithm to distribute PCR primer…

Subjects/Keywords: oligonukleotiidid; hübriidimine; polümeraasahelreaktsioon; biokiibid; modelleerimine (teadus); oligonucleotides; hybridization; polymeraze chain reaction; microarrays; modelling (science)

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Kaplinski, L. (2013). The application of oligonucleotide hybridization model for PCR and microarray optimization . (Thesis). Tartu University. Retrieved from http://hdl.handle.net/10062/32163

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Kaplinski, Lauris. “The application of oligonucleotide hybridization model for PCR and microarray optimization .” 2013. Thesis, Tartu University. Accessed January 20, 2021. http://hdl.handle.net/10062/32163.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Kaplinski, Lauris. “The application of oligonucleotide hybridization model for PCR and microarray optimization .” 2013. Web. 20 Jan 2021.

Vancouver:

Kaplinski L. The application of oligonucleotide hybridization model for PCR and microarray optimization . [Internet] [Thesis]. Tartu University; 2013. [cited 2021 Jan 20]. Available from: http://hdl.handle.net/10062/32163.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Kaplinski L. The application of oligonucleotide hybridization model for PCR and microarray optimization . [Thesis]. Tartu University; 2013. Available from: http://hdl.handle.net/10062/32163

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Rhodes University

13. Matereke, Lavious Tapiwa. Analysis of predictive power of binding affinity of PBM-derived sequences.

Degree: Faculty of Science, Biochemistry and Microbiology, 2015, Rhodes University

URL: http://hdl.handle.net/10962/d1018666

► A transcription factor (TF) is a protein that binds to specific DNA sequences as part of the initiation stage of transcription. Various methods of finding… (more)

▼ A transcription factor (TF) is a protein that binds to specific DNA sequences as part of the initiation stage of transcription. Various methods of finding these transcription factor binding sites (TFBS) have been developed. In vivo technologies analyze DNA binding regions known to have bound to a TF in a living cell. Most widely used in vivo methods at the moment are chromatin immunoprecipitation followed by deep sequencing (ChIP-seq) and DNase I hypersensitive sites sequencing. In vitro methods derive TFBS based on experiments with TFs and DNA usually in artificial settings or computationally. An example is the Protein Binding Microarray which uses artificially constructed DNA sequences to determine the short sequences that are most likely to bind to a TF. The major drawback of this approach is that binding of TFs in vivo is also dependent on other factors such as chromatin accessibility and the presence of cofactors. Therefore TFBS derived from the PBM technique might not resemble the true DNA binding sequences. In this work, we use PBM data from the UniPROBE motif database, ChIP-seq data and DNase I hypersensitive sites data. Using the Spearman’s rank correlation and area under receiver operating characteristic curve, we compare the enrichment scores which the PBM approach assigns to its identified sequences and the frequency of these sequences in likely binding regions and the human genome as a whole. We also use central motif enrichment analysis (CentriMo) to compare the enrichment of UniPROBE motifs with in vivo derived motifs (from the JASPAR CORE database) in their respective TF ChIP-seq peak region. CentriMo is applied to 14 TF ChIP-seq peak regions from different cell lines. We aim to establish if there is a relationship between the occurrences of UniPROBE 8-mer patterns in likely binding regions and their enrichment score and how well the in vitro derived motifs match in vivo binding specificity. We did not come out with a particular trend showing failure of the PBM approach to predict in vivo binding specificity. Our results show Ets1, Hnf4a and Tcf3 show prediction failure by the PBM technique in terms of our Spearman’s rank correlation for ChIP-seq data and central motif enrichment analysis. However, the PBM technique also matched the in vivo binding specificities of FoxA2, Pou2f2 and Mafk. Failure of the PBM approach was found to be a result of variability in the TF’s binding specificity, the presence of cofactors, narrow binding specificity and the presence ubiquitous binding patterns.

Subjects/Keywords: Transcription factors; Protein binding; DNA-binding proteins; Chromatin; Protein microarrays

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Matereke, L. T. (2015). Analysis of predictive power of binding affinity of PBM-derived sequences. (Thesis). Rhodes University. Retrieved from http://hdl.handle.net/10962/d1018666

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Matereke, Lavious Tapiwa. “Analysis of predictive power of binding affinity of PBM-derived sequences.” 2015. Thesis, Rhodes University. Accessed January 20, 2021. http://hdl.handle.net/10962/d1018666.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Matereke, Lavious Tapiwa. “Analysis of predictive power of binding affinity of PBM-derived sequences.” 2015. Web. 20 Jan 2021.

Vancouver:

Matereke LT. Analysis of predictive power of binding affinity of PBM-derived sequences. [Internet] [Thesis]. Rhodes University; 2015. [cited 2021 Jan 20]. Available from: http://hdl.handle.net/10962/d1018666.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Matereke LT. Analysis of predictive power of binding affinity of PBM-derived sequences. [Thesis]. Rhodes University; 2015. Available from: http://hdl.handle.net/10962/d1018666

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Rhodes University

14. Hlatshwayo, Nkosikhona Rejoyce. Comparison of protein binding microarray derived and ChIP-seq derived transcription factor binding DNA motifs.

Degree: Faculty of Science, Biochemistry and Microbiology, 2015, Rhodes University

URL: http://hdl.handle.net/10962/d1017907

► Transcription factors (TFs) are biologically important proteins that interact with transcription machinery and bind DNA regulatory sequences to regulate gene expression by modulating the synthesis… (more)

▼ Transcription factors (TFs) are biologically important proteins that interact with transcription machinery and bind DNA regulatory sequences to regulate gene expression by modulating the synthesis of the messenger RNA. The regulatory sequences comprise of short conserved regions of a specific length called motifs . TFs have very diverse roles in different cells and play a very significant role in development. TFs have been associated with carcinogenesis in various tissue types, as well as developmental and hormone response disorders. They may be responsible for the regulation of oncogenes and can be oncogenic. Consequently, understanding TF binding and knowing the motifs to which they bind is worthy of attention and research focus. Various projects have made the study of TF binding their main focus; nevertheless, much about TF binding remains confounding. Chromatin immunoprecipitation in conjunction with deep sequencing (ChIP-seq) techniques are a popular method used to investigate DNA-TF interactions in vivo. This procedure is followed by motif discovery and motif enrichment analysis using relevant tools. Protein Binding Microarrays (PBMs) are an in vitro method for investigating DNA-TF interactions. We use a motif enrichment analysis tools (CentriMo and AME) and an empirical quality assessment tool (Area under the ROC curve) to investigate which method yields motifs that are a true representation of in vivo binding. Motif enrichment analysis: On average, ChIP-seq derived motifs from the JASPAR Core database outperformed PBM derived ones from the UniPROBE mouse database. However, the performance of motifs derived using these two methods is not much different from each other when using CentriMo and AME. The E-values from Motif enrichment analysis were not too different from each other or 0. CentriMo showed that in 35 cases JASPAR Core ChIP-seq derived motifs outperformed UniPROBE mouse PBM derived motifs, while it was only in 11 cases that PBM derived motifs outperformed ChIP-seq derived motifs. AME showed that in 18 cases JASPAR Core ChIP-seq derived motifs did better, while only it was only in 3 cases that UniPROBE motifs outperformed ChIP-seq derived motifs. We could not distinguish the performance in 25 cases. Empirical quality assessment: Area under the ROC curve values computations followed by a two-sided t-test showed that there is no significant difference in the average performances of the motifs from the two databases (with 95% confidence, mean of differences=0.0088125 p-value= 0.4874, DF=47) .

Subjects/Keywords: Protein binding; DNA; DNA microarrays; Transcription factors; DNA-protein interactions; Gene regulatory networks

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APA (6^th Edition):

Hlatshwayo, N. R. (2015). Comparison of protein binding microarray derived and ChIP-seq derived transcription factor binding DNA motifs. (Thesis). Rhodes University. Retrieved from http://hdl.handle.net/10962/d1017907

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Hlatshwayo, Nkosikhona Rejoyce. “Comparison of protein binding microarray derived and ChIP-seq derived transcription factor binding DNA motifs.” 2015. Thesis, Rhodes University. Accessed January 20, 2021. http://hdl.handle.net/10962/d1017907.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Hlatshwayo, Nkosikhona Rejoyce. “Comparison of protein binding microarray derived and ChIP-seq derived transcription factor binding DNA motifs.” 2015. Web. 20 Jan 2021.

Vancouver:

Hlatshwayo NR. Comparison of protein binding microarray derived and ChIP-seq derived transcription factor binding DNA motifs. [Internet] [Thesis]. Rhodes University; 2015. [cited 2021 Jan 20]. Available from: http://hdl.handle.net/10962/d1017907.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Hlatshwayo NR. Comparison of protein binding microarray derived and ChIP-seq derived transcription factor binding DNA motifs. [Thesis]. Rhodes University; 2015. Available from: http://hdl.handle.net/10962/d1017907

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

15. 降籏, 友和. Fabrication and characterization of osteoconductive scaffold of recombinant peptide based on　human collagen type I and β-tri calcium phosphate nanoparticles.

Degree: 博士(歯学), 歯学, 2019, Hokkaido University

URL: http://hdl.handle.net/2115/77149

► Recombinant peptide based on human collagen type I has been introduced as a xeno-free polymer material for various tissue engineering approach. In this study, we… (more)

Subjects/Keywords: Bone tissue engineering; rat; reverse transcription polymerase chain reaction (RT-PCR)

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APA (6^th Edition):

降籏, �. (2019). Fabrication and characterization of osteoconductive scaffold of recombinant peptide based on　human collagen type I and β-tri calcium phosphate nanoparticles. (Doctoral Dissertation). Hokkaido University. Retrieved from http://hdl.handle.net/2115/77149

Chicago Manual of Style (16^th Edition):

降籏, 友和. “Fabrication and characterization of osteoconductive scaffold of recombinant peptide based on　human collagen type I and β-tri calcium phosphate nanoparticles.” 2019. Doctoral Dissertation, Hokkaido University. Accessed January 20, 2021. http://hdl.handle.net/2115/77149.

MLA Handbook (7^th Edition):

降籏, 友和. “Fabrication and characterization of osteoconductive scaffold of recombinant peptide based on　human collagen type I and β-tri calcium phosphate nanoparticles.” 2019. Web. 20 Jan 2021.

Vancouver:

降籏 �. Fabrication and characterization of osteoconductive scaffold of recombinant peptide based on　human collagen type I and β-tri calcium phosphate nanoparticles. [Internet] [Doctoral dissertation]. Hokkaido University; 2019. [cited 2021 Jan 20]. Available from: http://hdl.handle.net/2115/77149.

Council of Science Editors:

降籏 �. Fabrication and characterization of osteoconductive scaffold of recombinant peptide based on　human collagen type I and β-tri calcium phosphate nanoparticles. [Doctoral Dissertation]. Hokkaido University; 2019. Available from: http://hdl.handle.net/2115/77149

16. Negasi, Zerihun Hailemariam. Molekularbiologischer Nachweis und Charakterisierung von durch Zecken übertragenen Pathogenen bei Rindern im Südwesten Äthiopiens.

Degree: 2018, Freie Universität Berlin

URL: http://dx.doi.org/10.17169/refubium-61

► Zecken-übertragene Krankheiten (TBDs), welche 80 % der weltweiten Rinderpopulation betreffen, haben einen negativen Einfluss auf die Nutzviehwirtschaft auf der ganzen Welt. Das Vorkommen der Babesiose,… (more)

▼ Zecken-übertragene Krankheiten (TBDs), welche 80 % der weltweiten Rinderpopulation betreffen, haben einen negativen Einfluss auf die Nutzviehwirtschaft auf der ganzen Welt. Das Vorkommen der Babesiose, Anaplasmose, Herzwasserkrankheit und Theileriose bei Rindern in Äthiopien wurde beschrieben. Allerdings ist wenig bekannt über die Bedeutung und die Prävalenz dieser Krankheiten in den lokalen Rinderpopulationen Äthiopiens. Ziel dieser Dissertation war es daher, die Spezieszusammensetzung und die Prävalenz der von Zecken übertragenen Pathogene (TBP) in Rindern im Südwesten von Äthiopien zu bestimmen. Die Dissertation gliedert sich in zwei Studien. Die erste Studie beschäftigt sich mit der Evaluierung von sechs DNS-Extraktionsmethoden. Eine Zehner-Verdünnungsreihe von Rinderblut, das mit Babesia bovis, Theileria mutans, Anaplasma marginale oder Ehrlichia ruminantium infiziert war, wurde durch Verdünnung mit nicht infiziertem Blut hergestellt und auf FTA-Karten („Flinders-Technology-Associates“ cards) übertragen. Anschließend wurde die DNS unter Verwendung von sechs verschiedenen DNS-Extraktionsmethoden aus den FTA-Karten extrahiert. Zusätzlich wurde vergleichend DNS aus den Proben mit einem handelsüblichen Extraktions-Kit isoliert. Ziel war es, das optimale Protokoll für eine sich anschließende molekulare Erfassung der von Zecken übertragenen Pathogene durch PCR und „Reverse-Line-Blot-Hybridisierung“ (RLB) zu bestimmen. Die Ergebnisse dieser Studie zeigten, dass (i) unter den verglichenen Methoden die höchste Nachweisempfindlichkeit für die Erreger erreicht wurde, wenn die DNS aus FTAKarten unter Verwendung der FTA-Reinigungslösung, kombiniert mit der Elution mit 5% Chelex® 100-Harz, extrahiert wurde. Zweitens wurde die Nachweisgrenze verbessert, wenn mehrere Stanzproben von den FTA-Karten der gleichen Probe als Ausgangsmaterial für die DNS-Extraktion verwendet wurden, wobei die Verwendung von sechzehn 3 mm-Stanzproben sich als am praktischsten erwies. (iii) Vergleiche der Nachweisgrenzen von RLB und PCR für die durch Zecken übertragenen Pathogene für Rinder zeigten, dass die Empfindlichkeit der DNS-Extraktion aus den FTA-Karten zwischen verschiedenen TBPs variierte und die Empfindlichkeit der RLB-Hybridisierung in fast allen Fällen 10x höher war als im Vergleich zur Gelelektrophorese. In der zweiten Studie sollte die Prävalenz und Spezieszusammensetzung der TBPs in den lokalen Rinderpopulationen in der Region von Illubator, im Südwesten von Äthiopien, bestimmt werden. Die Extraktionsmethode mit der höchsten Nachweisempfindlichkeit (FTA-Reinigungslösung kombiniert mit 5% Chelex® 100-Harz) wurde in Kombination mit der PCR und einem RLB-Hybridisierungsassay für die Detektion eines breiten Spektrums von TBPs angewandt. Insgesamt wurden Blutproben von 392 Rinder auf die Erreger aus vier TBP-Gattungen untersucht (Babesia spp., Theileria spp., Anaplasma spp. und Ehrlichia spp.). Die Ergebnisse zeigen eine hohe Prävalenz mehrerer Rinder-TBPs in Südwest-Äthiopien, von denen einige eine große veterinärmedizinische Bedeutung… Advisors/Committee Members: male (gender), Clausen, Peter-Henning (firstReferee), Franz Josef Conraths (furtherReferee), Jabbar Sabir Ahmed (furtherReferee).

Subjects/Keywords: Tick-borne diseases; molecular epidemiology; DNA hybridization; polymerase chain reaction; cattle; Ethiopia; 600 Technik, Medizin, angewandte Wissenschaften::630 Landwirtschaft::630 Landwirtschaft und verwandte Bereiche

13 more images…

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APA (6^th Edition):

Negasi, Z. H. (2018). Molekularbiologischer Nachweis und Charakterisierung von durch Zecken übertragenen Pathogenen bei Rindern im Südwesten Äthiopiens. (Thesis). Freie Universität Berlin. Retrieved from http://dx.doi.org/10.17169/refubium-61

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Negasi, Zerihun Hailemariam. “Molekularbiologischer Nachweis und Charakterisierung von durch Zecken übertragenen Pathogenen bei Rindern im Südwesten Äthiopiens.” 2018. Thesis, Freie Universität Berlin. Accessed January 20, 2021. http://dx.doi.org/10.17169/refubium-61.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Negasi, Zerihun Hailemariam. “Molekularbiologischer Nachweis und Charakterisierung von durch Zecken übertragenen Pathogenen bei Rindern im Südwesten Äthiopiens.” 2018. Web. 20 Jan 2021.

Vancouver:

Negasi ZH. Molekularbiologischer Nachweis und Charakterisierung von durch Zecken übertragenen Pathogenen bei Rindern im Südwesten Äthiopiens. [Internet] [Thesis]. Freie Universität Berlin; 2018. [cited 2021 Jan 20]. Available from: http://dx.doi.org/10.17169/refubium-61.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Negasi ZH. Molekularbiologischer Nachweis und Charakterisierung von durch Zecken übertragenen Pathogenen bei Rindern im Südwesten Äthiopiens. [Thesis]. Freie Universität Berlin; 2018. Available from: http://dx.doi.org/10.17169/refubium-61

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Freie Universität Berlin

17. Richter, Nadine. Virological and molecular biological studies for the spreading and the relevance of EHV-5 infections in horses.

Degree: 2008, Freie Universität Berlin

URL: http://dx.doi.org/10.17169/refubium-12388

► The current dissertation is dedicated to the spreading and the relevance of the herpes virus type 5. The main question was whether EHV-5 is present… (more)

▼ The current dissertation is dedicated to the spreading and the relevance of the herpes virus type 5. The main question was whether EHV-5 is present in Germany, in which diagnostic materials and whether EHV-5 plays a decisive role in respiratory, ophthalmologic and unspecific symptoms. Furthermore, it was the objective to see, whether double infections with EHV-5 and EHV-1, EHV-2 or EHV-4 occur and whether vaccine and immune modulators have influence on the presence of EHV-5 At last the tissue- and cell tropism of EHV-5 was examined using nPCR and in-situ-hybridisation. There were two investigation groups: One group of clinically healthy horses, which consisted of 18 animals and a second group, which consisted of 107 sick horses. In total 125 horses were examined To study the tissue- and cell tropism nPCR and in-situ-hybridisation from post-mortem paraffin sections of conjunctival tissues from six different horses and PBMC from five different equines were conducted. EHV-5 was detected in 34 of 125 horses using nPCR. The virus was present in 6/18 clinically healthy horses in PBMC and in 28/107 clinically sick horses, especially in those with ophthalmologic symptoms (25/82) and in 3/19 horses with unspecific symptoms: EHV-5 was detected in 25/82 horses with ocular diseases in 18 cytobrushs (18/90), in two cotton wool swabs (2/11), in six PBMC (6/72) and in two nasal swabs (2/6). The number of the EHV-5 positive horses with ocular diseases does not match the number of the EHV-5 positive samples, because one or more samples (eye swabs, nasal swabs, PBMC) per horse were sent at a time and examined. EHV-5 was present in three horses with unspecific symptoms in two cytobrushs (2/90) and in one PBMC (1/72). Viral double infections of EHV-2 and EHV-5 were detected in three horses with ocular diseases (3/82) in three cytobrushs (3/90) and in two clinically healthy horses (2/18) in two PBMC (2/72). EHV-2 was detected in seven clinically diseased horses (7/107), thereof five ophthalmic equines (5/82), in three cytobrushs (3/90) and four cotton wool swabs (4/11), and in two clinically sound horses (2/18) in two PBMC (2/72). This work could not provide any new clues regarding the double infections with EHV-5 and EHV-1 or EHV-4. After a single treatment with Resequin NN Plus® and a triple application of the immune modulator Zylexis® none of the 18 clinically healthy horses, which were tested EHV-5 positive in PBMC before the therapy, were tested positive for the virus genome in PBMC. Fresh conjunctival tissues from six clinically healthy horses from the slaughterhouse were EHV-5-nPCR positive in one case. From all six cases post- mortem paraffin sections were tested for localization of the virus-DNA by in- situ-hybridisation using an EHV-5-Digoxigenin-labelled-genomic-probe. EHV-5-DNA was detected in cells of the lamina propria in sections of paraffin embedded conjunctiva of one horse. Since all horses were clinically healthy, one can conclude that EHV-5 persists in conjunctiva. PBMC from five clinically diseased horses were tested… Advisors/Committee Members: n (gender), PD Dr. Kerstin Borchers (firstReferee), Prof. Dr. Corinna Eule (furtherReferee), PD Dr. Michael Veit (furtherReferee).

Subjects/Keywords: horses; horse diseases; eye diseases; Equid Herpesvirus 5; Equid Herpesvirus 2; viral infections; polymerase chain reaction; sampling; in situ hybridization; 600 Technik, Medizin, angewandte Wissenschaften::630 Landwirtschaft

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Richter, N. (2008). Virological and molecular biological studies for the spreading and the relevance of EHV-5 infections in horses. (Thesis). Freie Universität Berlin. Retrieved from http://dx.doi.org/10.17169/refubium-12388

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Richter, Nadine. “Virological and molecular biological studies for the spreading and the relevance of EHV-5 infections in horses.” 2008. Thesis, Freie Universität Berlin. Accessed January 20, 2021. http://dx.doi.org/10.17169/refubium-12388.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Richter, Nadine. “Virological and molecular biological studies for the spreading and the relevance of EHV-5 infections in horses.” 2008. Web. 20 Jan 2021.

Vancouver:

Richter N. Virological and molecular biological studies for the spreading and the relevance of EHV-5 infections in horses. [Internet] [Thesis]. Freie Universität Berlin; 2008. [cited 2021 Jan 20]. Available from: http://dx.doi.org/10.17169/refubium-12388.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Richter N. Virological and molecular biological studies for the spreading and the relevance of EHV-5 infections in horses. [Thesis]. Freie Universität Berlin; 2008. Available from: http://dx.doi.org/10.17169/refubium-12388

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Utah

18. Hsieh, Fen-Fen. Development of a sensitive polymerase chain reaction assay for group A streptococci;.

Degree: MS;, Pathology;, 1995, University of Utah

URL: http://content.lib.utah.edu/cdm/singleitem/collection/etd1/id/1062/rec/308

► S. pyogenes causes many important human diseases. Rheumatic fever is a systemic inflammatory and nonsuppurative sequla of acute streptococcal pharyngitis that occurs in a small… (more)

Subjects/Keywords: Polymerase Chain Reaction; DNA

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APA (6^th Edition):

Hsieh, F. (1995). Development of a sensitive polymerase chain reaction assay for group A streptococci;. (Masters Thesis). University of Utah. Retrieved from http://content.lib.utah.edu/cdm/singleitem/collection/etd1/id/1062/rec/308

Chicago Manual of Style (16^th Edition):

Hsieh, Fen-Fen. “Development of a sensitive polymerase chain reaction assay for group A streptococci;.” 1995. Masters Thesis, University of Utah. Accessed January 20, 2021. http://content.lib.utah.edu/cdm/singleitem/collection/etd1/id/1062/rec/308.

MLA Handbook (7^th Edition):

Hsieh, Fen-Fen. “Development of a sensitive polymerase chain reaction assay for group A streptococci;.” 1995. Web. 20 Jan 2021.

Vancouver:

Hsieh F. Development of a sensitive polymerase chain reaction assay for group A streptococci;. [Internet] [Masters thesis]. University of Utah; 1995. [cited 2021 Jan 20]. Available from: http://content.lib.utah.edu/cdm/singleitem/collection/etd1/id/1062/rec/308.

Council of Science Editors:

Hsieh F. Development of a sensitive polymerase chain reaction assay for group A streptococci;. [Masters Thesis]. University of Utah; 1995. Available from: http://content.lib.utah.edu/cdm/singleitem/collection/etd1/id/1062/rec/308

University of the Western Cape

19. Kasu, Mohaimin. Validation and application of a highly discriminating and rapid 10-locus Y-STR DNA profiling system .

Degree: 2019, University of the Western Cape

URL: http://hdl.handle.net/11394/6760

► DNA profiling the male specific region on the Y-chromosome is fundamental to forensic practise. Its recognised as a powerful analytical tool for investigation of sexual… (more)

Subjects/Keywords: DNA profiling; Forensic genetics; Polymerase Chain Reaction; Rapidly mutating; Sexual assault

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APA (6^th Edition):

Kasu, M. (2019). Validation and application of a highly discriminating and rapid 10-locus Y-STR DNA profiling system . (Thesis). University of the Western Cape. Retrieved from http://hdl.handle.net/11394/6760

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Kasu, Mohaimin. “Validation and application of a highly discriminating and rapid 10-locus Y-STR DNA profiling system .” 2019. Thesis, University of the Western Cape. Accessed January 20, 2021. http://hdl.handle.net/11394/6760.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Kasu, Mohaimin. “Validation and application of a highly discriminating and rapid 10-locus Y-STR DNA profiling system .” 2019. Web. 20 Jan 2021.

Vancouver:

Kasu M. Validation and application of a highly discriminating and rapid 10-locus Y-STR DNA profiling system . [Internet] [Thesis]. University of the Western Cape; 2019. [cited 2021 Jan 20]. Available from: http://hdl.handle.net/11394/6760.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Kasu M. Validation and application of a highly discriminating and rapid 10-locus Y-STR DNA profiling system . [Thesis]. University of the Western Cape; 2019. Available from: http://hdl.handle.net/11394/6760

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Hong Kong University of Science and Technology

20. Mao, Mao. The role of poly (ADP-ribose) polymerase 1 in DNA damage-induced downregulation of the ubiquitin ligase CHFR.

Degree: 2014, Hong Kong University of Science and Technology

URL: http://repository.ust.hk/ir/Record/1783.1-83613 ; https://doi.org/10.14711/thesis-b1274538 ; http://repository.ust.hk/ir/bitstream/1783.1-83613/1/th_redirect.html

► CHFR (Checkpoint with Forkhead-associated and RING finger domains) is involved in a prophase checkpoint upon microtubule stress and is suppressed by DNA methylation in many… (more)

▼ CHFR (Checkpoint with Forkhead-associated and RING finger domains) is involved in a prophase checkpoint upon microtubule stress and is suppressed by DNA methylation in many cancer cell lines. Apart from its checkpoint functions and E₃ ubiquitin ligase activity, CHFR contains PAR-binding zinc-fingers which can interact with poly(ADP-ribose) (PAR) modifications. Poly(ADP-ribose) Polymerase1 (PARP1) is the most important member of PARP family. It is activated by DNA strand breaks, which then synthesizes a long and branched PAR network at DNA damage sites by auto-PARylation. This coordinates the recruitment of various downstream proteins involved in DNA damage response through affinity to PARylation or protein-protein interactions. Thus CHFR is also speculated to play a role in DNA damage responses through its PBZ motif. Previously, our group observed that CHFR was downregulated upon DNA damage induced by ionizing radiation or alkylating agent treatment, at the same time when PARP1 was activated. This downregulation could be inhibited by proteasome inhibitors or PARP inhibitors, indicating the involvement of a PARylation- and ubiquitin-dependent pathway. In my studies, I found that CHFR was downregulated not at the transcriptional level but dependent on auto-ubiquitination. Elevation of PARylation by PARG depletion could enhance the downregulation of CHFR. Collated with the fact that PARP inhibition stabilized CHFR but not its PBZ mutant, I speculated that CHFR downregulation was PARylation dependent. Then I demonstrated PARP1 depletion by siRNA or overexpression of PARP1 increased or decreased CHFR level, respectively, suggesting a role of PARP1 in this regulation. Co-immunoprecipitation results indicated an increased binding affinity between CHFR and PARP1, which could be abolished by PARP inhibitors or PBZ mutation. Finally, I utilized an in vivo ubiquitination assay to assess the ubiquitination status of CHFR and found that CHFR is more poly-ubiquitinated after alkylating agent treatment than after PARP inhibitor treatment. Taken together, I proposed a model that after DNA damage, CHFR is downregulated by auto-ubiquitination-mediated degradation through the binding to PARylation synthesized by PARP1.

Subjects/Keywords: ADP-ribosylation ; Polymerase chain reaction ; Cell cycle ; Regulation ; DNA damage

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Mao, M. (2014). The role of poly (ADP-ribose) polymerase 1 in DNA damage-induced downregulation of the ubiquitin ligase CHFR. (Thesis). Hong Kong University of Science and Technology. Retrieved from http://repository.ust.hk/ir/Record/1783.1-83613 ; https://doi.org/10.14711/thesis-b1274538 ; http://repository.ust.hk/ir/bitstream/1783.1-83613/1/th_redirect.html

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Mao, Mao. “The role of poly (ADP-ribose) polymerase 1 in DNA damage-induced downregulation of the ubiquitin ligase CHFR.” 2014. Thesis, Hong Kong University of Science and Technology. Accessed January 20, 2021. http://repository.ust.hk/ir/Record/1783.1-83613 ; https://doi.org/10.14711/thesis-b1274538 ; http://repository.ust.hk/ir/bitstream/1783.1-83613/1/th_redirect.html.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Mao, Mao. “The role of poly (ADP-ribose) polymerase 1 in DNA damage-induced downregulation of the ubiquitin ligase CHFR.” 2014. Web. 20 Jan 2021.

Vancouver:

Mao M. The role of poly (ADP-ribose) polymerase 1 in DNA damage-induced downregulation of the ubiquitin ligase CHFR. [Internet] [Thesis]. Hong Kong University of Science and Technology; 2014. [cited 2021 Jan 20]. Available from: http://repository.ust.hk/ir/Record/1783.1-83613 ; https://doi.org/10.14711/thesis-b1274538 ; http://repository.ust.hk/ir/bitstream/1783.1-83613/1/th_redirect.html.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Mao M. The role of poly (ADP-ribose) polymerase 1 in DNA damage-induced downregulation of the ubiquitin ligase CHFR. [Thesis]. Hong Kong University of Science and Technology; 2014. Available from: http://repository.ust.hk/ir/Record/1783.1-83613 ; https://doi.org/10.14711/thesis-b1274538 ; http://repository.ust.hk/ir/bitstream/1783.1-83613/1/th_redirect.html

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Western Ontario

21. Guha Roy, Debanjan. A new algorithm for primer design.

Degree: 2019, University of Western Ontario

URL: https://ir.lib.uwo.ca/etd/6643

► The Polymerase Chain Reaction (PCR) technology is widely used to create DNA copies. It has impacted many diverse fields including genetics, forensics, molecular paleontology, medical… (more)

Subjects/Keywords: DNA; Polymerase Chain Reaction; Primer3; bestPrimer; Other Computer Sciences

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APA (6^th Edition):

Guha Roy, D. (2019). A new algorithm for primer design. (Thesis). University of Western Ontario. Retrieved from https://ir.lib.uwo.ca/etd/6643

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Guha Roy, Debanjan. “A new algorithm for primer design.” 2019. Thesis, University of Western Ontario. Accessed January 20, 2021. https://ir.lib.uwo.ca/etd/6643.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Guha Roy, Debanjan. “A new algorithm for primer design.” 2019. Web. 20 Jan 2021.

Vancouver:

Guha Roy D. A new algorithm for primer design. [Internet] [Thesis]. University of Western Ontario; 2019. [cited 2021 Jan 20]. Available from: https://ir.lib.uwo.ca/etd/6643.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Guha Roy D. A new algorithm for primer design. [Thesis]. University of Western Ontario; 2019. Available from: https://ir.lib.uwo.ca/etd/6643

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Georgia

22. Thomas, Jesse Michael. Molecular surveillance for lymphoproliferative disease virus in hunter-killed wild turkeys (Meleagris gallopavo) from the eastern United States.

Degree: 2014, University of Georgia

URL: http://hdl.handle.net/10724/30033

► Three avian retroviruses can cause lymphoid tumors in galliforms, including avian leukosis virus, reticuloendotheliosis virus, and lymphoproliferative disease virus (LPDV). Historically, LPDV was considered a… (more)

Subjects/Keywords: Lymphoproliferative Disease Virus; LPDV, polymerase chain reaction; proviral DNA; wild turkey

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APA (6^th Edition):

Thomas, J. M. (2014). Molecular surveillance for lymphoproliferative disease virus in hunter-killed wild turkeys (Meleagris gallopavo) from the eastern United States. (Thesis). University of Georgia. Retrieved from http://hdl.handle.net/10724/30033

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Thomas, Jesse Michael. “Molecular surveillance for lymphoproliferative disease virus in hunter-killed wild turkeys (Meleagris gallopavo) from the eastern United States.” 2014. Thesis, University of Georgia. Accessed January 20, 2021. http://hdl.handle.net/10724/30033.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Thomas, Jesse Michael. “Molecular surveillance for lymphoproliferative disease virus in hunter-killed wild turkeys (Meleagris gallopavo) from the eastern United States.” 2014. Web. 20 Jan 2021.

Vancouver:

Thomas JM. Molecular surveillance for lymphoproliferative disease virus in hunter-killed wild turkeys (Meleagris gallopavo) from the eastern United States. [Internet] [Thesis]. University of Georgia; 2014. [cited 2021 Jan 20]. Available from: http://hdl.handle.net/10724/30033.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Thomas JM. Molecular surveillance for lymphoproliferative disease virus in hunter-killed wild turkeys (Meleagris gallopavo) from the eastern United States. [Thesis]. University of Georgia; 2014. Available from: http://hdl.handle.net/10724/30033

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Montana

23. Streeter, James M. Effect of DNA polymerases on the production of short tandem repeat stutter bands.

Degree: MS, 1999, University of Montana

URL: https://scholarworks.umt.edu/etd/9253

Subjects/Keywords: Polymerase chain reaction.; DNA fingerprints.

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APA (6^th Edition):

Streeter, J. M. (1999). Effect of DNA polymerases on the production of short tandem repeat stutter bands. (Masters Thesis). University of Montana. Retrieved from https://scholarworks.umt.edu/etd/9253

Chicago Manual of Style (16^th Edition):

Streeter, James M. “Effect of DNA polymerases on the production of short tandem repeat stutter bands.” 1999. Masters Thesis, University of Montana. Accessed January 20, 2021. https://scholarworks.umt.edu/etd/9253.

MLA Handbook (7^th Edition):

Streeter, James M. “Effect of DNA polymerases on the production of short tandem repeat stutter bands.” 1999. Web. 20 Jan 2021.

Vancouver:

Streeter JM. Effect of DNA polymerases on the production of short tandem repeat stutter bands. [Internet] [Masters thesis]. University of Montana; 1999. [cited 2021 Jan 20]. Available from: https://scholarworks.umt.edu/etd/9253.

Council of Science Editors:

Streeter JM. Effect of DNA polymerases on the production of short tandem repeat stutter bands. [Masters Thesis]. University of Montana; 1999. Available from: https://scholarworks.umt.edu/etd/9253

Universidade Estadual de Campinas

24. Conte, Fabio Frangiotti. Analise da expressão e localização do transcrito do gene EFHC1 no cerebro de roedores durante o desenvolvimento e no animal adulto: Analysis of the expression profile and distribution of EFHC1 gene transcript during rodent brain development and in the adult animal.

Degree: 2009, Universidade Estadual de Campinas

URL: http://repositorio.unicamp.br/jspui/handle/REPOSIP/316859

► Abstract: Epilepsy is a frequent condition that affects around 1.5% of general population and is considered worldwide as a public health problem. The term epilepsy… (more)

▼ Abstract: Epilepsy is a frequent condition that affects around 1.5% of general population and is considered worldwide as a public health problem. The term epilepsy refers to a group of chronic neurological disorders with different etiologies and prognostics. However, a common characteristic of all epilepsies is the recurrence of seizures. Juvenile myoclonic epilepsy (JME), one of the most common forms of epilepsy, is characterized by myoclonic seizures mainly at awakening. JME has been intensively studied at the molecular level. Recently, one of the putative causative genes for JME was cloned. This gene, called EFHC1, encodes a protein with 640 amino acids that has three DM10 domains, with unknown function, and a calcium binding motif, called Ef-Hand. EFHC1 protein associates with microtubules and, therefore, it actively participates in the cellular division process. In addition, it has been demonstrated that this protein induces apoptosis in cultured neurons through the association with an R-type voltage-dependent calcium channel (Cav2.3).To date, five different EFHC1 missense mutations identified in patients with JME were shown to decrease this proapoptotic function in cell models. The ortholog genes in mouse and rat, both named Efhc1, were also isolated. The murine gene encodes a protein with around 75KDa, homolog to Chlamydomonas reinhardtii Rib72 protein. Efhc1 protein presents its highest expression levels in tissues and organs that have mobile cilia and flagella, like testis, oviduct and, in the central nervous system, the ependymal cells. The aim of this study was to determine the distribution and expression profile of Efhc1 genes and evaluate the viability of functional studies by the modulation of the its expression during the development of the brain of mice and rats. Real time polymerase chain reaction (Real Time PCR) revealed that there is no difference in the expression of Efhc1 transcript between right and left hemispheres in both species. Western blot experiments corroborated this finding. In addition, the highest levels of Efhc1 mRNA were found at intra-uterine stages in mouse and in adulthood in rat. In common, there was a progressive decrease in Efhc1 expression from 1-day-old neonates to 14-days-old animals in both species. In situ hybridization studies showed that rat and mouse Efhc1 mRNAs are expressed in ependymal cells of ventricle walls. Our findings suggest that Efhc1 expression is more important during initial phases of brain development and that at this stage it could be involved in key developmental mechanisms underlying JME. The technique of RNA interference (RNAi) promotes a potent and specific gene silence and it was employed in this project to study the function of Efhc1 gene in cultured mammal cells and in the brain of mice in different developmental stages. This approach has been widely used to achieve this aim. Both for cultured cells assays as for in vivo experiments, the percentage of gene silence was evaluated with Real Time PCR and Western blot. In addition, negative controls… Advisors/Committee Members: UNIVERSIDADE ESTADUAL DE CAMPINAS (CRUESP), Lopes-Cendes, Íscia Teresinha, 1964- (advisor), Universidade Estadual de Campinas. Instituto de Biologia (institution), Programa de Pós-Graduação em Genética e Biologia Molecular (nameofprogram), Papes, Fabio (committee member), Sonatti, Maria de Fatima (committee member), Valle, Angela Cristina (committee member), Paço-Larson, Maria Luisa (committee member).

Subjects/Keywords: Epilepsia mioclônica juvenil; Expressão gênica; Reação em cadeia da polimerase; Hibridização in situ; Interferência de RNA; Juvenile myoclonic epilepsy; Gene expression; Polymerase chain reaction; In situ hybridization; RNA interference

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APA (6^th Edition):

Conte, F. F. (2009). Analise da expressão e localização do transcrito do gene EFHC1 no cerebro de roedores durante o desenvolvimento e no animal adulto: Analysis of the expression profile and distribution of EFHC1 gene transcript during rodent brain development and in the adult animal. (Thesis). Universidade Estadual de Campinas. Retrieved from http://repositorio.unicamp.br/jspui/handle/REPOSIP/316859

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Conte, Fabio Frangiotti. “Analise da expressão e localização do transcrito do gene EFHC1 no cerebro de roedores durante o desenvolvimento e no animal adulto: Analysis of the expression profile and distribution of EFHC1 gene transcript during rodent brain development and in the adult animal.” 2009. Thesis, Universidade Estadual de Campinas. Accessed January 20, 2021. http://repositorio.unicamp.br/jspui/handle/REPOSIP/316859.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Conte, Fabio Frangiotti. “Analise da expressão e localização do transcrito do gene EFHC1 no cerebro de roedores durante o desenvolvimento e no animal adulto: Analysis of the expression profile and distribution of EFHC1 gene transcript during rodent brain development and in the adult animal.” 2009. Web. 20 Jan 2021.

Vancouver:

Conte FF. Analise da expressão e localização do transcrito do gene EFHC1 no cerebro de roedores durante o desenvolvimento e no animal adulto: Analysis of the expression profile and distribution of EFHC1 gene transcript during rodent brain development and in the adult animal. [Internet] [Thesis]. Universidade Estadual de Campinas; 2009. [cited 2021 Jan 20]. Available from: http://repositorio.unicamp.br/jspui/handle/REPOSIP/316859.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Conte FF. Analise da expressão e localização do transcrito do gene EFHC1 no cerebro de roedores durante o desenvolvimento e no animal adulto: Analysis of the expression profile and distribution of EFHC1 gene transcript during rodent brain development and in the adult animal. [Thesis]. Universidade Estadual de Campinas; 2009. Available from: http://repositorio.unicamp.br/jspui/handle/REPOSIP/316859

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Florida

25. CRESAWN, STEVEN GAINES ( Author, Primary ). Genetic Inquiry into Vaccinia Virus Intermediate and Late Gene Regulation.

Degree: University of Florida

URL: https://ufdc.ufl.edu/UFE0010102

Subjects/Keywords: DNA; Enzymes; Genes; Genetic mutation; Genomes; In vitro fertilization; Peptide elongation factors; Polymerase chain reaction; RNA; Vaccinia virus

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APA (6^th Edition):

CRESAWN, STEVEN GAINES ( Author, P. ). (n.d.). Genetic Inquiry into Vaccinia Virus Intermediate and Late Gene Regulation. (Thesis). University of Florida. Retrieved from https://ufdc.ufl.edu/UFE0010102

Note: this citation may be lacking information needed for this citation format:
No year of publication.
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

CRESAWN, STEVEN GAINES ( Author, Primary ). “Genetic Inquiry into Vaccinia Virus Intermediate and Late Gene Regulation.” Thesis, University of Florida. Accessed January 20, 2021. https://ufdc.ufl.edu/UFE0010102.

Note: this citation may be lacking information needed for this citation format:
No year of publication.
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

CRESAWN, STEVEN GAINES ( Author, Primary ). “Genetic Inquiry into Vaccinia Virus Intermediate and Late Gene Regulation.” Web. 20 Jan 2021.

Note: this citation may be lacking information needed for this citation format:
No year of publication.

Vancouver:

CRESAWN, STEVEN GAINES ( Author P). Genetic Inquiry into Vaccinia Virus Intermediate and Late Gene Regulation. [Internet] [Thesis]. University of Florida; [cited 2021 Jan 20]. Available from: https://ufdc.ufl.edu/UFE0010102.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
No year of publication.

Council of Science Editors:

CRESAWN, STEVEN GAINES ( Author P). Genetic Inquiry into Vaccinia Virus Intermediate and Late Gene Regulation. [Thesis]. University of Florida; Available from: https://ufdc.ufl.edu/UFE0010102

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
No year of publication.

University of Florida

26. Febres-Rodriguez, Vicente Jose, 1964-. Molecular characterization of citrus tristeza virus genes and their use in plant transformation.

Degree: 1995, University of Florida

URL: https://ufdc.ufl.edu/AA00022440

Subjects/Keywords: Activating transcription factors; Amino acids; Antiserum; DNA; Open reading frames; Pitting; Plasmids; Polymerase chain reaction; Proteins; RNA; Plant Pathology thesis Ph. D

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APA (6^th Edition):

Febres-Rodriguez, Vicente Jose, 1. (1995). Molecular characterization of citrus tristeza virus genes and their use in plant transformation. (Thesis). University of Florida. Retrieved from https://ufdc.ufl.edu/AA00022440

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Febres-Rodriguez, Vicente Jose, 1964-. “Molecular characterization of citrus tristeza virus genes and their use in plant transformation.” 1995. Thesis, University of Florida. Accessed January 20, 2021. https://ufdc.ufl.edu/AA00022440.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Febres-Rodriguez, Vicente Jose, 1964-. “Molecular characterization of citrus tristeza virus genes and their use in plant transformation.” 1995. Web. 20 Jan 2021.

Vancouver:

Febres-Rodriguez, Vicente Jose 1. Molecular characterization of citrus tristeza virus genes and their use in plant transformation. [Internet] [Thesis]. University of Florida; 1995. [cited 2021 Jan 20]. Available from: https://ufdc.ufl.edu/AA00022440.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Febres-Rodriguez, Vicente Jose 1. Molecular characterization of citrus tristeza virus genes and their use in plant transformation. [Thesis]. University of Florida; 1995. Available from: https://ufdc.ufl.edu/AA00022440

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Loma Linda University

27. Brown, Amber. Quantitative Characterization of Conserved Noncoding Regions by Flow Cytometry.

Degree: MS, Basic Sciences, 2014, Loma Linda University

URL: https://scholarsrepository.llu.edu/etd/200

► Spatial and temporal regulation of developmental gene transcription often involves regulatory sequences found in noncoding DNA separate from the gene’s promoter. Conservation of a… (more)

Subjects/Keywords: Biochemistry; Biochemistry, Biophysics, and Structural Biology; Medicine and Health Sciences; Flow Cytometry; Gene Expression Profiling; Reverse Transcriptase Polymerase Chain Reaction; Fibroblast Growth Factors; Receptor Protein-Tyrosine Kinases; Receptors - Retinoic Acid; Mesoderm; Electroporation; Limb buds; Models - Biological; Development Gene Transcription; Noncoding DNA; Morphogenesis; CNR-Green flourescent protein; Fluorescent Microscopy Localization

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Brown, A. (2014). Quantitative Characterization of Conserved Noncoding Regions by Flow Cytometry. (Thesis). Loma Linda University. Retrieved from https://scholarsrepository.llu.edu/etd/200

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Brown, Amber. “Quantitative Characterization of Conserved Noncoding Regions by Flow Cytometry.” 2014. Thesis, Loma Linda University. Accessed January 20, 2021. https://scholarsrepository.llu.edu/etd/200.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Brown, Amber. “Quantitative Characterization of Conserved Noncoding Regions by Flow Cytometry.” 2014. Web. 20 Jan 2021.

Vancouver:

Brown A. Quantitative Characterization of Conserved Noncoding Regions by Flow Cytometry. [Internet] [Thesis]. Loma Linda University; 2014. [cited 2021 Jan 20]. Available from: https://scholarsrepository.llu.edu/etd/200.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Brown A. Quantitative Characterization of Conserved Noncoding Regions by Flow Cytometry. [Thesis]. Loma Linda University; 2014. Available from: https://scholarsrepository.llu.edu/etd/200

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Universiteit Utrecht

28. Radonjić, Marijana. Genome-wide analysis of transcription regulation during the cell quiescence cycle.

Degree: 2006, Universiteit Utrecht

URL: http://dspace.library.uu.nl:8080/handle/1874/12619

► All organisms have cells that are capable of exiting the normal cell cycle and entering a non-proliferative state termed quiescence or G0. Most eukaryotic cells,… (more)

Subjects/Keywords: Geneeskunde; transcription regulation; quiescence; G0; DNA microarrays; chip on chip; RNA polymerase II; transcription factors; S. cerevisiae

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Radonjić, M. (2006). Genome-wide analysis of transcription regulation during the cell quiescence cycle. (Doctoral Dissertation). Universiteit Utrecht. Retrieved from http://dspace.library.uu.nl:8080/handle/1874/12619

Chicago Manual of Style (16^th Edition):

Radonjić, Marijana. “Genome-wide analysis of transcription regulation during the cell quiescence cycle.” 2006. Doctoral Dissertation, Universiteit Utrecht. Accessed January 20, 2021. http://dspace.library.uu.nl:8080/handle/1874/12619.

MLA Handbook (7^th Edition):

Radonjić, Marijana. “Genome-wide analysis of transcription regulation during the cell quiescence cycle.” 2006. Web. 20 Jan 2021.

Vancouver:

Radonjić M. Genome-wide analysis of transcription regulation during the cell quiescence cycle. [Internet] [Doctoral dissertation]. Universiteit Utrecht; 2006. [cited 2021 Jan 20]. Available from: http://dspace.library.uu.nl:8080/handle/1874/12619.

Council of Science Editors:

Radonjić M. Genome-wide analysis of transcription regulation during the cell quiescence cycle. [Doctoral Dissertation]. Universiteit Utrecht; 2006. Available from: http://dspace.library.uu.nl:8080/handle/1874/12619

29. Radonjić, Marijana. Genome-wide analysis of transcription regulation during the cell quiescence cycle.

Degree: 2006, University Utrecht

URL: https://dspace.library.uu.nl/handle/1874/12619 ; URN:NBN:NL:UI:10-1874-12619 ; urn:isbn:90-393-4334-9 ; URN:NBN:NL:UI:10-1874-12619 ; https://dspace.library.uu.nl/handle/1874/12619

► All organisms have cells that are capable of exiting the normal cell cycle and entering a non-proliferative state termed quiescence or G0. Most eukaryotic cells,… (more)

Subjects/Keywords: transcription regulation; quiescence; G0; DNA microarrays; chip on chip; RNA polymerase II; transcription factors; S. cerevisiae

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Radonjić, M. (2006). Genome-wide analysis of transcription regulation during the cell quiescence cycle. (Doctoral Dissertation). University Utrecht. Retrieved from https://dspace.library.uu.nl/handle/1874/12619 ; URN:NBN:NL:UI:10-1874-12619 ; urn:isbn:90-393-4334-9 ; URN:NBN:NL:UI:10-1874-12619 ; https://dspace.library.uu.nl/handle/1874/12619

Chicago Manual of Style (16^th Edition):

Radonjić, Marijana. “Genome-wide analysis of transcription regulation during the cell quiescence cycle.” 2006. Doctoral Dissertation, University Utrecht. Accessed January 20, 2021. https://dspace.library.uu.nl/handle/1874/12619 ; URN:NBN:NL:UI:10-1874-12619 ; urn:isbn:90-393-4334-9 ; URN:NBN:NL:UI:10-1874-12619 ; https://dspace.library.uu.nl/handle/1874/12619.

MLA Handbook (7^th Edition):

Radonjić, Marijana. “Genome-wide analysis of transcription regulation during the cell quiescence cycle.” 2006. Web. 20 Jan 2021.

Vancouver:

Radonjić M. Genome-wide analysis of transcription regulation during the cell quiescence cycle. [Internet] [Doctoral dissertation]. University Utrecht; 2006. [cited 2021 Jan 20]. Available from: https://dspace.library.uu.nl/handle/1874/12619 ; URN:NBN:NL:UI:10-1874-12619 ; urn:isbn:90-393-4334-9 ; URN:NBN:NL:UI:10-1874-12619 ; https://dspace.library.uu.nl/handle/1874/12619.

Council of Science Editors:

Radonjić M. Genome-wide analysis of transcription regulation during the cell quiescence cycle. [Doctoral Dissertation]. University Utrecht; 2006. Available from: https://dspace.library.uu.nl/handle/1874/12619 ; URN:NBN:NL:UI:10-1874-12619 ; urn:isbn:90-393-4334-9 ; URN:NBN:NL:UI:10-1874-12619 ; https://dspace.library.uu.nl/handle/1874/12619

30. Radonjić, Marijana. Genome-wide analysis of transcription regulation during the cell quiescence cycle.

Degree: 2006, University Utrecht

URL: https://dspace.library.uu.nl/handle/1874/12619 ; URN:NBN:NL:UI:10-1874-12619 ; 1874/12619 ; urn:isbn:9039343349 ; URN:NBN:NL:UI:10-1874-12619 ; https://dspace.library.uu.nl/handle/1874/12619

► All organisms have cells that are capable of exiting the normal cell cycle and entering a non-proliferative state termed quiescence or G0. Most eukaryotic cells,… (more)

Subjects/Keywords: transcription regulation; quiescence; G0; DNA microarrays; chip on chip; RNA polymerase II; transcription factors; S. cerevisiae

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Radonjić, M. (2006). Genome-wide analysis of transcription regulation during the cell quiescence cycle. (Doctoral Dissertation). University Utrecht. Retrieved from https://dspace.library.uu.nl/handle/1874/12619 ; URN:NBN:NL:UI:10-1874-12619 ; 1874/12619 ; urn:isbn:9039343349 ; URN:NBN:NL:UI:10-1874-12619 ; https://dspace.library.uu.nl/handle/1874/12619

Chicago Manual of Style (16^th Edition):

Radonjić, Marijana. “Genome-wide analysis of transcription regulation during the cell quiescence cycle.” 2006. Doctoral Dissertation, University Utrecht. Accessed January 20, 2021. https://dspace.library.uu.nl/handle/1874/12619 ; URN:NBN:NL:UI:10-1874-12619 ; 1874/12619 ; urn:isbn:9039343349 ; URN:NBN:NL:UI:10-1874-12619 ; https://dspace.library.uu.nl/handle/1874/12619.

MLA Handbook (7^th Edition):

Radonjić, Marijana. “Genome-wide analysis of transcription regulation during the cell quiescence cycle.” 2006. Web. 20 Jan 2021.

Vancouver:

Radonjić M. Genome-wide analysis of transcription regulation during the cell quiescence cycle. [Internet] [Doctoral dissertation]. University Utrecht; 2006. [cited 2021 Jan 20]. Available from: https://dspace.library.uu.nl/handle/1874/12619 ; URN:NBN:NL:UI:10-1874-12619 ; 1874/12619 ; urn:isbn:9039343349 ; URN:NBN:NL:UI:10-1874-12619 ; https://dspace.library.uu.nl/handle/1874/12619.

Council of Science Editors:

Radonjić M. Genome-wide analysis of transcription regulation during the cell quiescence cycle. [Doctoral Dissertation]. University Utrecht; 2006. Available from: https://dspace.library.uu.nl/handle/1874/12619 ; URN:NBN:NL:UI:10-1874-12619 ; 1874/12619 ; urn:isbn:9039343349 ; URN:NBN:NL:UI:10-1874-12619 ; https://dspace.library.uu.nl/handle/1874/12619

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Open Access Theses and Dissertations