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Texas A&M University
1.
Rodriguez Jr., Jaime Eleazar.
Ecology of a Rodent-Tick-Pathogen Community in East-Central Texas.
Degree: MS, Wildlife and Fisheries Sciences, 2014, Texas A&M University
URL: http://hdl.handle.net/1969.1/153486 
► Rodent species worldwide are critical to the maintenance of tick-borne disease systems because they serve as hosts for ticks and reservoirs for zoonotic pathogens. To…
(more)
▼ Rodent species worldwide are critical to the maintenance of
tick-
borne disease systems because they serve as hosts for ticks and reservoirs for zoonotic
pathogens. To learn more about native fauna that may be involved in enzootic transmission of
pathogens that can cause
tick-
borne diseases (TBDs), a mark-recapture study of rodents was conducted in Brazos County in east-central Texas. My objectives were to: (i) describe the species richness and seasonal activity of rodents; (ii) characterize rodent infestation with ticks over time; and (iii) determine the infection prevalence of rodents and ticks with selected zoonotic
tick-
borne pathogens.
For nineteen months, small mammals were live-trapped two nights per month and subjected to blood and ear biopsy collections. All captured mammals were checked for the presence of ticks, which were removed for diagnostic testing. Additionally, drag sampling was conducted to collect ticks from the vegetation.
Five rodent species (Sigmodon hispidus, Reithrodontomys fulvescens, Peromyscus leucopus, P. gossypinus, and Baiomys taylori) were captured over the course of the study. A large increase in S. hispidus capture success was seen in fall 2013, reflecting the characteristic population booms exhibited by this species. Two
tick species - Amblyomma maculatum and Ixodes scapularis - were found infesting the rodents at low levels (2.33%). No ticks were found in over 14,500 meters of drag sampling the vegetation.
In an analysis of 698 ear biopsies, 3.2% of the specimens were positive for Borrelia miyamotoi, a spirochete that has recently been shown to cause relapsing fever in humans. One specimen (0.1%) was found to be infected with B. lonestari. No ticks were found to be infected with Borrelia. However, 4.3% of the larval A. maculatum pools were positive for a rickettsial endosymbiont. One larval A. maculatum pool and one I. scapularis nymph were found to be infected with Rickettsia monacensis, the causative agent for a Mediterranean spotted fever-like illness in Europe and North Africa. This study identifies novel TBDs in the southern United States and exposes the need for further study of TBD ecology, especially in understudied areas.
Advisors/Committee Members: Light, Jessica E (advisor), Hamer, Sarah A (advisor), Grant, William (committee member).
Subjects/Keywords: Rodent; Tick; Pathogen; Tick-borne pathogens
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APA (6th Edition):
Rodriguez Jr., J. E. (2014). Ecology of a Rodent-Tick-Pathogen Community in East-Central Texas. (Masters Thesis). Texas A&M University. Retrieved from http://hdl.handle.net/1969.1/153486
Chicago Manual of Style (16th Edition):
Rodriguez Jr., Jaime Eleazar. “Ecology of a Rodent-Tick-Pathogen Community in East-Central Texas.” 2014. Masters Thesis, Texas A&M University. Accessed January 22, 2021.
http://hdl.handle.net/1969.1/153486.
MLA Handbook (7th Edition):
Rodriguez Jr., Jaime Eleazar. “Ecology of a Rodent-Tick-Pathogen Community in East-Central Texas.” 2014. Web. 22 Jan 2021.
Vancouver:
Rodriguez Jr. JE. Ecology of a Rodent-Tick-Pathogen Community in East-Central Texas. [Internet] [Masters thesis]. Texas A&M University; 2014. [cited 2021 Jan 22].
Available from: http://hdl.handle.net/1969.1/153486.
Council of Science Editors:
Rodriguez Jr. JE. Ecology of a Rodent-Tick-Pathogen Community in East-Central Texas. [Masters Thesis]. Texas A&M University; 2014. Available from: http://hdl.handle.net/1969.1/153486
University of Pretoria
2.
[No author].
Molecular characterization of tick-borne pathogens of
domestic dogs from communal areas in Botswana
.
Degree: 2012, University of Pretoria
URL: http://upetd.up.ac.za/thesis/available/etd-05242012-144516/
► The occurrence of tick-borne pathogens in dogs on communal land in Botswana was studied using Reverse Line Blot (RLB). Eighty blood samples were collected from…
(more)
▼ The occurrence of
tick-
borne pathogens in dogs on
communal land in Botswana was studied using Reverse Line Blot
(RLB). Eighty blood samples were collected from dogs that underwent
a spay and neuter program in Maun, Botswana. These were sent to the
Department of Veterinary Tropical Diseases, University of Pretoria,
and were stored at the Molecular Laboratory. DNA was extracted from
the samples followed by the Polymerase Chain Reaction (PCR) and the
RLB. Six samples were found to be positive for Ehrlichia canis. Two
of the samples were positive for a concurrent infection of E. canis
and Ehrlichia ruminantium. There was one sample that was positive
for the non-pathogenic Ehrlichia sp. Omatjenne. Although six
samples were positive for the Babesia/Theileria catch-all probe,
none hybridised with any of the species-specific probes.
Copyright
Advisors/Committee Members: Prof B L Penzhorn (advisor), Dr P T Matjila (advisor).
Subjects/Keywords: Domestic dogs;
Botswana;
Tick-borne pathogens;
UCTD
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APA (6th Edition):
author], [. (2012). Molecular characterization of tick-borne pathogens of
domestic dogs from communal areas in Botswana
. (Masters Thesis). University of Pretoria. Retrieved from http://upetd.up.ac.za/thesis/available/etd-05242012-144516/
Chicago Manual of Style (16th Edition):
author], [No. “Molecular characterization of tick-borne pathogens of
domestic dogs from communal areas in Botswana
.” 2012. Masters Thesis, University of Pretoria. Accessed January 22, 2021.
http://upetd.up.ac.za/thesis/available/etd-05242012-144516/.
MLA Handbook (7th Edition):
author], [No. “Molecular characterization of tick-borne pathogens of
domestic dogs from communal areas in Botswana
.” 2012. Web. 22 Jan 2021.
Vancouver:
author] [. Molecular characterization of tick-borne pathogens of
domestic dogs from communal areas in Botswana
. [Internet] [Masters thesis]. University of Pretoria; 2012. [cited 2021 Jan 22].
Available from: http://upetd.up.ac.za/thesis/available/etd-05242012-144516/.
Council of Science Editors:
author] [. Molecular characterization of tick-borne pathogens of
domestic dogs from communal areas in Botswana
. [Masters Thesis]. University of Pretoria; 2012. Available from: http://upetd.up.ac.za/thesis/available/etd-05242012-144516/
Texas A&M University
3.
Galan, Aleyda Patricia.
Baseline Biodiversity Assessment of Small Mammals, Ticks, and Tick-borne Pathogens in South Texas.
Degree: MS, Wildlife and Fisheries Sciences, 2016, Texas A&M University
URL: http://hdl.handle.net/1969.1/159043
► Texas is a large state comprised of 10 ecological regions that provide diverse habitats to a variety of organisms. Stewardship programs throughout the state maintain…
(more)
▼ Texas is a large state comprised of 10 ecological regions that provide diverse habitats to a variety of organisms. Stewardship programs throughout the state maintain healthy habitats for organisms to occupy; yet often these properties are difficult to access, which leads to unknown true organismal diversity, presence, and habitat associations, particularly in South Texas. Additionally, organisms such as small mammals are hosts for ticks and
tick-
borne pathogens (TBPs). Therefore, through the East Foundation’s stewardship program, I created a baseline biodiversity assessment of small mammals, ticks, and
tick-
borne pathogens in Jim Hogg, Kenedy, Starr, and Willacy counties. I utilized several capture techniques including Sherman live traps, Macabee traps, and mist netting across three field seasons. To investigate TBPs presence, ticks and rodent ear biopsies were screened for the pathogen genera Borrelia and Rickettsia using PCR and DNA sequencing.
I captured 398 terrestrial, fossorial, and volant small mammals representing 18 species. Of these small mammals, 367 were terrestrial small mammals that were inspected for ticks. A total of 306 rodent ear biopsies were screened for TBPs, of which all were negative for Borrelia and Rickettsia. All ticks were negative for Borrelia and Rickettsia. Additionally, surveys for Texas pocket gophers revealed associations between their burrow systems and herpetofauna. A total of 125 herpetofauna were observed utilizing Texas pocket gopher mounds across East Foundation properties. This baseline assessment of small mammals, ticks, and
tick-
borne pathogens will serve as a reference point for future research efforts.
Advisors/Committee Members: Light, Jessica E (advisor), Voelker, Gary (committee member), Hamer, Sarah A (committee member), Campbell, Tyler A (committee member).
Subjects/Keywords: small mammals; ticks; tick-borne pathogens
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CSE |
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APA (6th Edition):
Galan, A. P. (2016). Baseline Biodiversity Assessment of Small Mammals, Ticks, and Tick-borne Pathogens in South Texas. (Masters Thesis). Texas A&M University. Retrieved from http://hdl.handle.net/1969.1/159043
Chicago Manual of Style (16th Edition):
Galan, Aleyda Patricia. “Baseline Biodiversity Assessment of Small Mammals, Ticks, and Tick-borne Pathogens in South Texas.” 2016. Masters Thesis, Texas A&M University. Accessed January 22, 2021.
http://hdl.handle.net/1969.1/159043.
MLA Handbook (7th Edition):
Galan, Aleyda Patricia. “Baseline Biodiversity Assessment of Small Mammals, Ticks, and Tick-borne Pathogens in South Texas.” 2016. Web. 22 Jan 2021.
Vancouver:
Galan AP. Baseline Biodiversity Assessment of Small Mammals, Ticks, and Tick-borne Pathogens in South Texas. [Internet] [Masters thesis]. Texas A&M University; 2016. [cited 2021 Jan 22].
Available from: http://hdl.handle.net/1969.1/159043.
Council of Science Editors:
Galan AP. Baseline Biodiversity Assessment of Small Mammals, Ticks, and Tick-borne Pathogens in South Texas. [Masters Thesis]. Texas A&M University; 2016. Available from: http://hdl.handle.net/1969.1/159043
University of Pretoria
4.
Sibanda, Donald Ray.
Molecular
characterization of tick-borne pathogens of domestic dogs from
communal areas in Botswana.
Degree: Veterinary Tropical
Diseases, 2011, University of Pretoria
URL: http://hdl.handle.net/2263/24948
► The occurrence of tick-borne pathogens in dogs on communal land in Botswana was studied using Reverse Line Blot (RLB). Eighty blood samples were collected from…
(more)
▼ The occurrence of
tick-
borne pathogens in dogs on
communal land in Botswana was studied using Reverse Line Blot
(RLB). Eighty blood samples were collected from dogs that underwent
a spay and neuter program in Maun, Botswana. These were sent to the
Department of Veterinary Tropical Diseases, University of Pretoria,
and were stored at the Molecular Laboratory. DNA was extracted from
the samples followed by the Polymerase Chain Reaction (PCR) and the
RLB. Six samples were found to be positive for Ehrlichia canis. Two
of the samples were positive for a concurrent infection of E. canis
and Ehrlichia ruminantium. There was one sample that was positive
for the non-pathogenic Ehrlichia sp. Omatjenne. Although six
samples were positive for the Babesia/Theileria catch-all probe,
none hybridised with any of the species-specific probes.
Copyright
Advisors/Committee Members: Penzhorn, Barend Louis (advisor), Matjila, P.T. (Paul Tshepo) (advisor).
Subjects/Keywords: Domestic
dogs;
Botswana; Tick-borne
pathogens;
UCTD
Record Details
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APA ·
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Manager
APA (6th Edition):
Sibanda, D. R. (2011). Molecular
characterization of tick-borne pathogens of domestic dogs from
communal areas in Botswana. (Masters Thesis). University of Pretoria. Retrieved from http://hdl.handle.net/2263/24948
Chicago Manual of Style (16th Edition):
Sibanda, Donald Ray. “Molecular
characterization of tick-borne pathogens of domestic dogs from
communal areas in Botswana.” 2011. Masters Thesis, University of Pretoria. Accessed January 22, 2021.
http://hdl.handle.net/2263/24948.
MLA Handbook (7th Edition):
Sibanda, Donald Ray. “Molecular
characterization of tick-borne pathogens of domestic dogs from
communal areas in Botswana.” 2011. Web. 22 Jan 2021.
Vancouver:
Sibanda DR. Molecular
characterization of tick-borne pathogens of domestic dogs from
communal areas in Botswana. [Internet] [Masters thesis]. University of Pretoria; 2011. [cited 2021 Jan 22].
Available from: http://hdl.handle.net/2263/24948.
Council of Science Editors:
Sibanda DR. Molecular
characterization of tick-borne pathogens of domestic dogs from
communal areas in Botswana. [Masters Thesis]. University of Pretoria; 2011. Available from: http://hdl.handle.net/2263/24948
University of Pretoria
5.
Matjila, P.T. (Paul Tshepo).
Molecular detection and characterization of tick-borne
pathogens of dogs
.
Degree: 2009, University of Pretoria
URL: http://upetd.up.ac.za/thesis/available/etd-01212009-122421/
► This thesis focuses on the molecular characterization of tick-borne parasites of dogs in South Africa. Emphasis is placed on Babesia, Erhlichia, incidental and novel parasite…
(more)
▼ This thesis focuses on the molecular
characterization of
tick-
borne parasites of dogs in South Africa.
Emphasis is placed on Babesia, Erhlichia, incidental and novel
parasite infections that may cause morbidity or mortality in
infected dogs. An outbreak of canine babesiosis in the Netherlands
is also reported in this thesis. Molecular techniques were employed
to isolate, amplify and characterize genomic DNA of these parasites
to species level. During preliminary screening of blood samples
collected from various sites in the country, that included the
Onderstepoort Veterinary Academic Hospital, SPCAs and private
clinics throughout seven provinces in South Africa, it was
discovered that domestic dogs harboured a wide variety of
tick-
borne pathogens. The most frequently encountered parasites in
South Africa were Babesia rossi, a novel Theileria species of dogs,
B. vogeli and Ehrlichia canis respectively. The parasites occurred
as single or mixed infections. Incidental infections that included
B. gibsoni and Trypanosoma congolense were also detected using PCR.
Although it was anticipated that zoonotic Ehrlichial infections of
dogs would be detected, none were found. Babesia vogeli was
reported for the first time in South Africa although, without any
clinical significance. An outbreak of autochthonous canine
babesiosis in the Netherlands was confirmed to have been caused by
Babesia canis. Dermacentor reticulates was implicated in the
transmission of the parasite to naïve dogs. Clinical significance
of B. rossi and the novel Theileria sp. of dogs was evaluated.
Babesia rossi was found to be of significant clinical importance.
Genotyping of B. rossi isolates revealed that parasite genotypes
could be correlated to disease phenotype. Additionally, specific
genotypes could also be associated with fatalities. Although the
characterization of the Theileria sp. in dogs was a first report in
South Africa, the clinical significance of this infection in dogs
appeared to be poorly resolved. The dangers of having non-endemic
species becoming established in South Africa was highlighted with
the incidental finding of a B. gibsoni infection in an imported
dog. The results of this thesis have shown therefore that
populations of dogs that live in tickendemic areas are exposed to
single or multiple
tick-
borne pathogens. These
pathogens continue
to cause morbidity and mortality in susceptible dogs. Correct
diagnosis (supported by molecular diagnostic tools) followed by
appropriate treatment offers a better understanding and management
of these
tick-
borne pathogens. Preventative measures should be
fully evaluated and applied to prevent these
tick-
borne pathogens
from adversely affecting the canine population in South Africa and
elsewhere.
Advisors/Committee Members: Prof B L Penzhorn (advisor), Prof F Jongejan (advisor).
Subjects/Keywords: Tick-borne pathogens;
South africa;
Molecular;
Dogs;
UCTD
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Manager
APA (6th Edition):
Matjila, P. T. (. T. (2009). Molecular detection and characterization of tick-borne
pathogens of dogs
. (Doctoral Dissertation). University of Pretoria. Retrieved from http://upetd.up.ac.za/thesis/available/etd-01212009-122421/
Chicago Manual of Style (16th Edition):
Matjila, P T (Paul Tshepo). “Molecular detection and characterization of tick-borne
pathogens of dogs
.” 2009. Doctoral Dissertation, University of Pretoria. Accessed January 22, 2021.
http://upetd.up.ac.za/thesis/available/etd-01212009-122421/.
MLA Handbook (7th Edition):
Matjila, P T (Paul Tshepo). “Molecular detection and characterization of tick-borne
pathogens of dogs
.” 2009. Web. 22 Jan 2021.
Vancouver:
Matjila PT(T. Molecular detection and characterization of tick-borne
pathogens of dogs
. [Internet] [Doctoral dissertation]. University of Pretoria; 2009. [cited 2021 Jan 22].
Available from: http://upetd.up.ac.za/thesis/available/etd-01212009-122421/.
Council of Science Editors:
Matjila PT(T. Molecular detection and characterization of tick-borne
pathogens of dogs
. [Doctoral Dissertation]. University of Pretoria; 2009. Available from: http://upetd.up.ac.za/thesis/available/etd-01212009-122421/
University of Tennessee – Knoxville
6.
Mays, Sarah Elizabeth.
Trapping Methods for Ixodid Ticks and Pathogen Associations of <i>Amblyomma maculatum</i> (Gulf Coast Tick) in Western Tennessee.
Degree: MS, Entomology and Plant Pathology, 2015, University of Tennessee – Knoxville
URL: https://trace.tennessee.edu/utk_gradthes/3337
► Ticks are vectors of disease agents and pests of humans and animals. Various methods are used for tick monitoring and pathogen surveillance to assess…
(more)
▼ Ticks are vectors of disease agents and pests of humans and animals. Various methods are used for
tick monitoring and pathogen surveillance to assess
tick distributions, pathogen prevalence and control measures, such as monitoring the changing geographic distribution of the Gulf Coast
tick,
Amblyomma maculatum. This project (1) compared the effectiveness of six trapping methods for the collection of hard (Ixodid) ticks in a typical grassland-forest habitat in southwestern Tennessee, and (2) examined pathogen associations of
A. maculatum collected in western Tennessee.
To compare trapping methods across time and habitat types, a temporal study was conducted in 2013 and a habitat study was conducted in 2014. Conventional
tick collection methods (dragging, flagging, dry ice trapping, and sweep-netting) and novel methods (carbon dioxide (CO
2)-reinforced dragging and flagging) were compared across five monthly sampling periods. Dragging, CO
2 dragging, CO
2 flagging and dry ice trapping were then compared across four habitat types (grassland, upland deciduous, bottomland deciduous, and coniferous). Significant interactions between trapping method and sampling period (2013) and between trapping method and habitat (2014) were identified. In both studies, the novel methods were comparable to their conventional counterparts; the addition of CO
2 did not significantly increase the number of ticks collected. Dry ice trapping and dragging were effective methods of
tick collection across time and habitat types, and were among the most effective methods for all species collected.
To detect
pathogens associated with
A. maculatum and identify the best surveillance methods for monitoring infected ticks, questing and host-feeding
A. maculatum (n = 265) collected in the 2013 and 2014 trapping studies and other concurrent studies were PCR-screened for
Rickettsia,
Ehrlichia, and
Borrelia species. Of the
A. maculatum screened, none were
Borrelia positive, 2 were
Ehrlichia positive, and 60 were infected with
R. parkeri (a pathogenic
Rickettsia). No particular surveillance technique (e.g. habitat type or collection source) was significantly more effective for detection of infected
A. maculatum. The results of this project demonstrate the importance of monitoring and surveillance methods based upon habitat, target species, and research objectives, and the need for continued monitoring and surveillance of ticks, including
A. maculatum.
Advisors/Committee Members: Rebecca T. Trout Fryxell, Allan E. Houston, Graham J. Hickling, Ernest C. Bernard.
Subjects/Keywords: Ixodidae; Ehrlichia; Rickettsia; Trapping; Tick-borne pathogens; Entomology
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Manager
APA (6th Edition):
Mays, S. E. (2015). Trapping Methods for Ixodid Ticks and Pathogen Associations of <i>Amblyomma maculatum</i> (Gulf Coast Tick) in Western Tennessee. (Thesis). University of Tennessee – Knoxville. Retrieved from https://trace.tennessee.edu/utk_gradthes/3337
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Mays, Sarah Elizabeth. “Trapping Methods for Ixodid Ticks and Pathogen Associations of <i>Amblyomma maculatum</i> (Gulf Coast Tick) in Western Tennessee.” 2015. Thesis, University of Tennessee – Knoxville. Accessed January 22, 2021.
https://trace.tennessee.edu/utk_gradthes/3337.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Mays, Sarah Elizabeth. “Trapping Methods for Ixodid Ticks and Pathogen Associations of <i>Amblyomma maculatum</i> (Gulf Coast Tick) in Western Tennessee.” 2015. Web. 22 Jan 2021.
Vancouver:
Mays SE. Trapping Methods for Ixodid Ticks and Pathogen Associations of <i>Amblyomma maculatum</i> (Gulf Coast Tick) in Western Tennessee. [Internet] [Thesis]. University of Tennessee – Knoxville; 2015. [cited 2021 Jan 22].
Available from: https://trace.tennessee.edu/utk_gradthes/3337.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Mays SE. Trapping Methods for Ixodid Ticks and Pathogen Associations of <i>Amblyomma maculatum</i> (Gulf Coast Tick) in Western Tennessee. [Thesis]. University of Tennessee – Knoxville; 2015. Available from: https://trace.tennessee.edu/utk_gradthes/3337
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Freie Universität Berlin
7.
Hagedorn, Peter.
Analysis of Ixodes, Haemaphysalis and Dermacentor ticks for tick-borne
pathogens around Berlin.
Degree: 2013, Freie Universität Berlin
URL: http://dx.doi.org/10.17169/refubium-8980
► Tick-borne pathogens are responsible for several human and livestock diseases with occasionally severe consequences. Therefore, it is relevant for the public health system of a…
(more)
▼ Tick-
borne pathogens are responsible for several human and livestock diseases
with occasionally severe consequences. Therefore, it is relevant for the
public health system of a country to analyse the potential risk for
tick-
borne
infections to increase the awareness and introduce control measures. In
Germany surveillance of
tick-
borne diseases is usually limited to
tick-
borne
encephalitis (TBE) virus, while other important
pathogens are omitted. Patchy
information on TBE incidence and
tick distribution in the country is
available, with most of the studies focused on areas in the Southern and
Western part of Germany. Likewise, little is known about the distribution of
tick species and the harboured
pathogens in the area of Berlin. In this study
we collected larvae, nymphs and adult ticks in seven representative sampling
sites around Berlin in a monthly planned routine over 28 months. Only month
with snow cover or sub-zero temperatures when no
tick activity was expected
were excluded. Based on the total number of collected ticks the different
boroughs where ticks were collected were attributed to classes of
tick
densities followed by the additional analysis of the presence of different
tick-
borne pathogens (Borrelia burgdorferi sensu lato, Rickettsia spp.,
Babesia spp., and Anaplasma phagocytophilum) in the collected ticks, using new
commercially available assays for this purpose. The results of these
investigations were validated with investigations done with conventional
molecularbiological methods. The results presented provide valuable data for
the estimation of the presence of ticks and the risk of infection by
tick-
borne pathogens in the Berlin area. By broadening the collection to the larval
stage, it was possible to find a possible explanation why Berlin does not
belong to TBE risk areas. The detection of borrelia pathogenic for humans in
D. reticulatus supports the idea that D. reticulatus could be a source of
borrelia infections in humans. The detection of a lot of rickettsia species
illustrates that diseases caused by rickettsia should be taken into account
and physicians should display increased alertness to these diseases. To our
knowledge this is the first time Rickettsia raoultii has been revealed in I.
ricinus in Germany. The confirmation of Babesia and Anaplasma phagocytophilum
in ticks collected around Berlin demonstrates that the appearance of
babesiosis and human granulocytic anaplasmosis in this area has to be also
considered. In the area of Brieskow-Finkenheerd Haemaphysalis concinna
appeared as an established population with a high number of ticks of all
stages. To identify the possible transmission of human
pathogens by
Haemaphysalis concinna further investigations are needed.
Advisors/Committee Members: [email protected] (contact), m (gender), Prof. Dr. Matthias Niedrig (RKI/HUB) (firstReferee), Prof. Dr. Rupert Mutzel (FUB) (furtherReferee).
Subjects/Keywords: Tick-borne pathogens; Ixodes ricinus; Dermacentor reticulatus; Borrelia burgdorferi; Babesia spp.; Anaplasma phagocytophilum;
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APA ·
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CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Hagedorn, P. (2013). Analysis of Ixodes, Haemaphysalis and Dermacentor ticks for tick-borne
pathogens around Berlin. (Thesis). Freie Universität Berlin. Retrieved from http://dx.doi.org/10.17169/refubium-8980
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Hagedorn, Peter. “Analysis of Ixodes, Haemaphysalis and Dermacentor ticks for tick-borne
pathogens around Berlin.” 2013. Thesis, Freie Universität Berlin. Accessed January 22, 2021.
http://dx.doi.org/10.17169/refubium-8980.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Hagedorn, Peter. “Analysis of Ixodes, Haemaphysalis and Dermacentor ticks for tick-borne
pathogens around Berlin.” 2013. Web. 22 Jan 2021.
Vancouver:
Hagedorn P. Analysis of Ixodes, Haemaphysalis and Dermacentor ticks for tick-borne
pathogens around Berlin. [Internet] [Thesis]. Freie Universität Berlin; 2013. [cited 2021 Jan 22].
Available from: http://dx.doi.org/10.17169/refubium-8980.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Hagedorn P. Analysis of Ixodes, Haemaphysalis and Dermacentor ticks for tick-borne
pathogens around Berlin. [Thesis]. Freie Universität Berlin; 2013. Available from: http://dx.doi.org/10.17169/refubium-8980
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
University of Pretoria
8.
Matjila, P.T. (Paul
Tshepo).
Molecular
detection and characterization of tick-borne pathogens of
dogs.
Degree: Veterinary Tropical
Diseases, 2009, University of Pretoria
URL: http://hdl.handle.net/2263/24372
► This thesis focuses on the molecular characterization of tick-borne parasites of dogs in South Africa. Emphasis is placed on Babesia, Erhlichia, incidental and novel parasite…
(more)
▼ This thesis focuses on the molecular characterization of
tick-
borne parasites of dogs in South Africa. Emphasis is placed on
Babesia, Erhlichia, incidental and novel parasite infections that
may cause morbidity or mortality in infected dogs. An outbreak of
canine babesiosis in the Netherlands is also reported in this
thesis. Molecular techniques were employed to isolate, amplify and
characterize genomic DNA of these parasites to species level.
During preliminary screening of blood samples collected from
various sites in the country, that included the Onderstepoort
Veterinary Academic Hospital, SPCAs and private clinics throughout
seven provinces in South Africa, it was discovered that domestic
dogs harboured a wide variety of
tick-
borne pathogens. The most
frequently encountered parasites in South Africa were Babesia
rossi, a novel Theileria species of dogs, B. vogeli and Ehrlichia
canis respectively. The parasites occurred as single or mixed
infections. Incidental infections that included B. gibsoni and
Trypanosoma congolense were also detected using PCR. Although it
was anticipated that zoonotic Ehrlichial infections of dogs would
be detected, none were found. Babesia vogeli was reported for the
first time in South Africa although, without any clinical
significance. An outbreak of autochthonous canine babesiosis in the
Netherlands was confirmed to have been caused by Babesia canis.
Dermacentor reticulates was implicated in the transmission of the
parasite to naïve dogs. Clinical significance of B. rossi and the
novel Theileria sp. of dogs was evaluated. Babesia rossi was found
to be of significant clinical importance. Genotyping of B. rossi
isolates revealed that parasite genotypes could be correlated to
disease phenotype. Additionally, specific genotypes could also be
associated with fatalities. Although the characterization of the
Theileria sp. in dogs was a first report in South Africa, the
clinical significance of this infection in dogs appeared to be
poorly resolved. The dangers of having non-endemic species becoming
established in South Africa was highlighted with the incidental
finding of a B. gibsoni infection in an imported dog. The results
of this thesis have shown therefore that populations of dogs that
live in tickendemic areas are exposed to single or multiple
tick-
borne pathogens. These
pathogens continue to cause morbidity
and mortality in susceptible dogs. Correct diagnosis (supported by
molecular diagnostic tools) followed by appropriate treatment
offers a better understanding and management of these
tick-
borne
pathogens. Preventative measures should be fully evaluated and
applied to prevent these
tick-
borne pathogens from adversely
affecting the canine population in South Africa and
elsewhere.
Advisors/Committee Members: Penzhorn, Barend Louis (advisor), Jongejan, Frans (advisor).
Subjects/Keywords: Tick-borne
pathogens; South
africa;
Molecular;
Dogs;
UCTD
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APA (6th Edition):
Matjila, P. T. (. (2009). Molecular
detection and characterization of tick-borne pathogens of
dogs. (Doctoral Dissertation). University of Pretoria. Retrieved from http://hdl.handle.net/2263/24372
Chicago Manual of Style (16th Edition):
Matjila, P T (Paul. “Molecular
detection and characterization of tick-borne pathogens of
dogs.” 2009. Doctoral Dissertation, University of Pretoria. Accessed January 22, 2021.
http://hdl.handle.net/2263/24372.
MLA Handbook (7th Edition):
Matjila, P T (Paul. “Molecular
detection and characterization of tick-borne pathogens of
dogs.” 2009. Web. 22 Jan 2021.
Vancouver:
Matjila PT(. Molecular
detection and characterization of tick-borne pathogens of
dogs. [Internet] [Doctoral dissertation]. University of Pretoria; 2009. [cited 2021 Jan 22].
Available from: http://hdl.handle.net/2263/24372.
Council of Science Editors:
Matjila PT(. Molecular
detection and characterization of tick-borne pathogens of
dogs. [Doctoral Dissertation]. University of Pretoria; 2009. Available from: http://hdl.handle.net/2263/24372
Freie Universität Berlin
9.
Rehman, Abdul.
Epidemiologie von Zecken und durch Zecken übertragene Pathogene in den ariden
und semiariden agrarökologischen Zonen Pakistans.
Degree: 2017, Freie Universität Berlin
URL: http://dx.doi.org/10.17169/refubium-7207
► Zecken und durch Zecken übertragene Krankheiten haben einen großen Einfluss auf die Tiergesundheit und den Lebensunterhalt der Halter von Nutztieren, besonders in Entwicklungsländern. Das Klima…
(more)
▼ Zecken und durch Zecken übertragene Krankheiten haben einen großen Einfluss
auf die Tiergesundheit und den Lebensunterhalt der Halter von Nutztieren,
besonders in Entwicklungsländern. Das Klima Pakistans ist ideal für Zecken,
dennoch mangelt es an systematischen Studien, die sich mit diesen Parasiten
und den von ihnen in diesem Land übertragenen Krankheiten beschäftigen. Um die
Verbreitung der Zecken besser zu verstehen, wurde das Land mit Hilfe von
Global-Aridity Daten in fünf agrarökologische Zonen unterteilt. Daten von 108
landwirtschaftlichen Betrieben aus 9 Bezirken sind in diese Studie
eingeflossen, womit die ariden und semiariden agrarökologischen Zonen der
Provinz Punjab abgedeckt werden. Zecken wurden in jedem Betrieb von zwei
zufällig ausgewählten Tieren jeder vorkommenden Wiederkäuerart gesammelt (194
Wasserbüffel, 179 Rinder, 80 Ziegen und 18 Schafe) und in 70% Ethanol
gelagert. Die morphologische Bestimmung der Zecken erfolgte mit taxonomischen
Standardschlüsseln und Multikey Software, einem computerbasierten polychotomen
Schlüssel. Diese Bestimmung wurde bestätigt durch Sequenzierung eines
Teilfragments des zweiten Internal Transcribed Spacers (ITS2) und der
Untereinheit 1 des Cytochromoxidase (cox1) Gens von zufällig gewählten
Individuen jeder Art, was die Übereinstimmung morphologischer und molekularer
Daten für die Bestimmung der vier gefundenen Zeckenarten bestätigt. Die
Verbreitung der Zecken in den verschiedenen agrarökologischen Zonen war
signifikant verschieden (P = 0,037). Es gab keinen Betrieb ohne Zecken.
Insgesamt wurden 3.807 Schildzecken (12 Larven, 1231 Nymphen, 1303 Weibchen
und 1261 Männchen) aus vier Arten gesammelt: Hyalomma anatolicum (n = 3021;
79,3 %), Rhipicephalus microplus (n = 715; 18,8%), Hyalomma dromedarii (n =
41; 1,0%) und Rhipicephalus turanicus (n = 30; 0,9 %). R. microplus war die
häufigste Art in der semiariden Zone, während Hy. anatolicum die häufigste
Zecke der ariden Zone war. Hy. dromedarii und Rh. turanicus wurden nur in der
ariden Zone gefunden. In allen Bezirken wurden mehrere Arten gefunden, außer
in Multan, wo es nur Hy. anatolicum gab. Der Gesamtanteil der mit Zecken
befallenen Tiere betrug 78,3 % (369/471) und war signifikant verschieden (P <
0,001; χ² = 126,9) unter den Tierarten. Am größten war der Anteil bei Rindern
(89,9%), gefolgt von Wasserbüffeln (81,4%), Ziegen (60%) und Schafen (11,1%).
Der mittlere Zeckenbefall, 43 Zecken pro Tier, von 27 bis 67 reichend, war
signifikant verschieden (P < 0,001) unter den Tierarten und zwar am höchsten
bei Rindern (58), gefolgt von Wasserbüffeln (38), Ziegen (19) und Schafen
(4,5). Bei den großen Wiederkäuern hatten alte Tiere mehr Zecken als jüngere
(Wasserbüffel P = 0,02; Rind P = 0,002). Es wurde ferner beobachtet, dass
Weibchen mehr Zecken hatten als Männchen (Wasserbüffel P = 0,002; Rind P <
0,001; Ziege P = 0,014; Schaf P = 0,02). Die Befallsintensität war signifikant
niedriger bei einheimischen Tieren als bei exotischen (P = 0,001) und
Kreuzungsrassen (P = 0,001), aber der Unterschied zwischen exotischen und…
Advisors/Committee Members: m (gender), Prof. Dr. Franz J. Conraths (firstReferee), Prof. Dr. Peter-Henning Clausen (furtherReferee), Univ.-Prof. Dr. Marcus G. Doherr (furtherReferee).
Subjects/Keywords: Ticks; tick-borne pathogens; anaplasmoses; ehrlichioses; rickettsial diseases; babesiosis; theileriosis; risk factors; livestock; 600 Technik, Medizin, angewandte Wissenschaften::630 Landwirtschaft::630 Landwirtschaft und verwandte Bereiche
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APA ·
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CSE |
Export
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Manager
APA (6th Edition):
Rehman, A. (2017). Epidemiologie von Zecken und durch Zecken übertragene Pathogene in den ariden
und semiariden agrarökologischen Zonen Pakistans. (Thesis). Freie Universität Berlin. Retrieved from http://dx.doi.org/10.17169/refubium-7207
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Rehman, Abdul. “Epidemiologie von Zecken und durch Zecken übertragene Pathogene in den ariden
und semiariden agrarökologischen Zonen Pakistans.” 2017. Thesis, Freie Universität Berlin. Accessed January 22, 2021.
http://dx.doi.org/10.17169/refubium-7207.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Rehman, Abdul. “Epidemiologie von Zecken und durch Zecken übertragene Pathogene in den ariden
und semiariden agrarökologischen Zonen Pakistans.” 2017. Web. 22 Jan 2021.
Vancouver:
Rehman A. Epidemiologie von Zecken und durch Zecken übertragene Pathogene in den ariden
und semiariden agrarökologischen Zonen Pakistans. [Internet] [Thesis]. Freie Universität Berlin; 2017. [cited 2021 Jan 22].
Available from: http://dx.doi.org/10.17169/refubium-7207.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Rehman A. Epidemiologie von Zecken und durch Zecken übertragene Pathogene in den ariden
und semiariden agrarökologischen Zonen Pakistans. [Thesis]. Freie Universität Berlin; 2017. Available from: http://dx.doi.org/10.17169/refubium-7207
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
10.
Gondard, Mathilde.
A la découverte des agents pathogènes et microorganismes des tiques par séquençage de nouvelle génération et QPCR microfluidique à haut débit : Screening of tick-borne pathogens and microorganisms in caribbean ticks by next generation sequencing and high-throughput microfluidic real-time PCR.
Degree: Docteur es, Sciences de la vie et de la santé, 2017, Université Paris-Est
URL: http://www.theses.fr/2017PESC1017
► Les maladies à transmission vectorielle sont dues à des agents pathogènes transmis par des arthropodes hématophages. Ces vecteurs assurent une transmission active (mécanique ou biologique)…
(more)
▼ Les maladies à transmission vectorielle sont dues à des agents pathogènes transmis par des arthropodes hématophages. Ces vecteurs assurent une transmission active (mécanique ou biologique) d’un agent infectieux d’un vertébré vers un autre vertébré. A l’échelle mondiale, les tiques sont responsables de la transmission de la plus grande variété d’agents pathogènes, elles transmettent des microorganismes responsables de maladies bactériennes (borréliose de Lyme, rickettsioses) ou parasitaires (babésioses, theilérioses), ou même virales (encéphalite à tiques).Les Antilles se situent au cœur de la zone Néotropicale des Caraïbes, et constituent une zone à risque pour l’émergence de maladies vectorielles en raison des conditions climatiques favorables aux vecteurs et des échanges intercontinentaux importants (flux illégal d’animaux, oiseaux migrateurs,…). La situation épidémiologique de la zone Caraïbe vis-à-vis des maladies transmises par les tiques est très peu documentée. Les études menées sur le terrain portent essentiellement sur des agents pathogènes affectant les animaux comme Ehrlichia ruminantium, Babesia (bovis et bigemina) et Anaplasma marginale et sont donc loin de pouvoir répondre aux questions concernant le risque d’émergence ou de réémergence de maladies à tique. Ainsi, il est nécessaire et urgent de développer des outils efficaces de surveillance épidémiologique qui permettraient la détection des agents pathogènes, nouveaux, connus ou non suspectés présents dans les tiques. C’est dans ce contexte d’amélioration des performances de veille sanitaire des maladies à tiques dans les Caraïbes que prend place le projet de thèse. La visée de la thèse était de faire un état des lieux des agents pathogènes d’intérêt médical et vétérinaire présents dans les tiques caribéennes à l’aide de techniques de détection à haut débit. Pour cela nous avons d’abord réalisé un séquençage à haut débit d’ARN extraits de tiques collectées en Guadeloupe et en Martinique afin de réaliser un inventaire sans a priori des agents pathogènes (bactéries, parasites, et virus) présents. Cette analyse a permis de mettre en évidence une grande diversité en microorganismes pathogènes au sein de nos échantillons, révélant également la présence de quatre virus appartenant à de nouveaux genres viraux récemment décrits et associés aux arthropodes. Les informations obtenues via le séquençage, additionnées aux données disponibles dans la littérature ont permis de constituer ainsi une liste des agents pathogènes transmis par les tiques nécessitant une surveillance sanitaire dans les caraïbes. A partir de ce répertoire nous avons développé un système de dépistage à haut-débit d’agents infectieux applicable à toute la zone des caraïbes. L’outil de détection est un support microfluidique de type puce à ADN, basé sur la technologie BioMarkTM dynamic arrays (Fluidigm Corporation) qui permet de réaliser de la PCR en temps réel à haut débit afin de détecter simultanément 48 à 96 cibles au sein de 48 à 96 échantillons. Deux puces ont été développées, une…
Advisors/Committee Members: Vayssier-Taussat, Muriel (thesis director), Albina, Emmanuel (thesis director).
Subjects/Keywords: Agents pathogènes; Tique; QPCR microfluidique à haut débit; Sequençage de nouvelle generation; Epidemiologie; Caraïbes; Tick-Borne pathogens; Ticks; High-Throughput microfluidic real-Time PCR; Next generation sequencing; Epidemiology; Caribbean
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APA ·
Chicago ·
MLA ·
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CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Gondard, M. (2017). A la découverte des agents pathogènes et microorganismes des tiques par séquençage de nouvelle génération et QPCR microfluidique à haut débit : Screening of tick-borne pathogens and microorganisms in caribbean ticks by next generation sequencing and high-throughput microfluidic real-time PCR. (Doctoral Dissertation). Université Paris-Est. Retrieved from http://www.theses.fr/2017PESC1017
Chicago Manual of Style (16th Edition):
Gondard, Mathilde. “A la découverte des agents pathogènes et microorganismes des tiques par séquençage de nouvelle génération et QPCR microfluidique à haut débit : Screening of tick-borne pathogens and microorganisms in caribbean ticks by next generation sequencing and high-throughput microfluidic real-time PCR.” 2017. Doctoral Dissertation, Université Paris-Est. Accessed January 22, 2021.
http://www.theses.fr/2017PESC1017.
MLA Handbook (7th Edition):
Gondard, Mathilde. “A la découverte des agents pathogènes et microorganismes des tiques par séquençage de nouvelle génération et QPCR microfluidique à haut débit : Screening of tick-borne pathogens and microorganisms in caribbean ticks by next generation sequencing and high-throughput microfluidic real-time PCR.” 2017. Web. 22 Jan 2021.
Vancouver:
Gondard M. A la découverte des agents pathogènes et microorganismes des tiques par séquençage de nouvelle génération et QPCR microfluidique à haut débit : Screening of tick-borne pathogens and microorganisms in caribbean ticks by next generation sequencing and high-throughput microfluidic real-time PCR. [Internet] [Doctoral dissertation]. Université Paris-Est; 2017. [cited 2021 Jan 22].
Available from: http://www.theses.fr/2017PESC1017.
Council of Science Editors:
Gondard M. A la découverte des agents pathogènes et microorganismes des tiques par séquençage de nouvelle génération et QPCR microfluidique à haut débit : Screening of tick-borne pathogens and microorganisms in caribbean ticks by next generation sequencing and high-throughput microfluidic real-time PCR. [Doctoral Dissertation]. Université Paris-Est; 2017. Available from: http://www.theses.fr/2017PESC1017
RMIT University
11.
Johnstone-Robertson, S.
Disease emergence and dynamics on biologically motivated contact networks.
Degree: 2017, RMIT University
URL: http://researchbank.rmit.edu.au/view/rmit:162413
► Infectious disease transmission requires that epidemiologically relevant contact occurs between infectious and susceptible individuals. Thus, for mathematical models to accurately predict disease emergence and dynamics…
(more)
▼ Infectious disease transmission requires that epidemiologically relevant contact occurs between infectious and susceptible individuals. Thus, for mathematical models to accurately predict disease emergence and dynamics they must incorporate the contact patterns responsible for transmission. In this context, this thesis investigates how the level of contact detail included in an infectious disease model influences its predictions. Three models are considered. The first investigates infections spreading through territorial populations, with potential canine rabies spread in Australian wild dogs a case study. Two factors governing wild dog contacts are considered: geographic distance and heterogeneous wild dog behaviour. Not including spatial constraints results in a model that overestimates the probability of an epidemic and that fails to generate the outcome 'rate of spread'. Conversely, not incorporating heterogeneous dog behaviour results in a model that underestimates the probability an epidemic will occur. The second model investigates tick-borne pathogen spread between ticks and vertebrate hosts. Key features of tick feeding behaviour include: tick aggregation on hosts, co-aggregation of larval and nymphal ticks on the same hosts, and co-feeding. Co-aggregation increases R0. Models failing to incorporate tick co-aggregation will therefore underestimate the likelihood of pathogen emergence, especially in geographic regions and seasons where larval burden is high and for pathogens mainly transmitted during co-feeding. The third model investigates the effect of clustering (triangle and square contact patterns) on the spread of infection through social networks. Clustering reduces R0 and the magnitude of the reduction increases with higher transmission probabilities. Models that fail to incorporate clustering will overestimate the likelihood of disease establishment, especially for highly transmissible diseases. In conclusion, the three disease models collectively reveal model predictions are improved and additional outcomes are generated by the inclusion of realistic host contact patterns. These findings reinforce the value of incorporating biologically-faithful contact patterns into infectious disease models.
Subjects/Keywords: Fields of Research; contact patterns; infectious disease model; basic reproduction number; R0; canine rabies; Australian wild dogs; dingos; dingoes; spatial model; heterogeneity; tick-borne pathogens; aggregation; co-aggregation; co-feeding; clustering; social networks
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APA ·
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APA (6th Edition):
Johnstone-Robertson, S. (2017). Disease emergence and dynamics on biologically motivated contact networks. (Thesis). RMIT University. Retrieved from http://researchbank.rmit.edu.au/view/rmit:162413
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Johnstone-Robertson, S. “Disease emergence and dynamics on biologically motivated contact networks.” 2017. Thesis, RMIT University. Accessed January 22, 2021.
http://researchbank.rmit.edu.au/view/rmit:162413.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Johnstone-Robertson, S. “Disease emergence and dynamics on biologically motivated contact networks.” 2017. Web. 22 Jan 2021.
Vancouver:
Johnstone-Robertson S. Disease emergence and dynamics on biologically motivated contact networks. [Internet] [Thesis]. RMIT University; 2017. [cited 2021 Jan 22].
Available from: http://researchbank.rmit.edu.au/view/rmit:162413.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Johnstone-Robertson S. Disease emergence and dynamics on biologically motivated contact networks. [Thesis]. RMIT University; 2017. Available from: http://researchbank.rmit.edu.au/view/rmit:162413
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Western Kentucky University
12.
Tackett, Kristina.
The Lyme Disease Spirochete, <i>Borrelia burgdorferi</i>, in Tick Species Collected from Raccoons (<i>Procyon lotor</i>) and Opossums (<i>Didelphis virginiana</i>) Trapped in the Warren and Barren Counties of South Central Kentucky.
Degree: MS, Department of Biology, 2009, Western Kentucky University
URL: https://digitalcommons.wku.edu/theses/118
► The incidence of tick-borne zoonoses such as Ehrlichiosis, Rocky Mountain Spotted Fever, and Lyme disease has steadily increased in the southeastern United States in recent…
(more)
▼ The incidence of
tick-
borne zoonoses such as Ehrlichiosis, Rocky Mountain Spotted Fever, and Lyme disease has steadily increased in the southeastern United States in recent years. According to the Centers for Disease Control and Prevention (CDC), the southeastern states accounted for 1,200 of the 27,000 total cases of Lyme disease reported in the U.S. in 2007. Although <i>Ixodes scapularis</i> is the most commonly recognized vector for the Lyme disease spirochete <i>Borrelia burgdorferi</i>, <i>Dermacentor variabilis</i> (a common vector for Rocky Mountain Spotted Fever) also has been shown to be a viable host for this pathogen. The purpose of the present study was to use PCR and DNA sequencing technologies to determine if <i>Borrelia burgdorferi sensu lato</i> is present in ticks and whole blood samples removed from raccoons and opossums trapped in south-central Kentucky.
Raccoons and opossums were trapped in Barren and Warren counties of Kentucky between June 2007 and June 2008. Ticks were removed and stored in 70% ethanol. Sterile blood samples were collected into three 10 ml tubes containing the anticoagulant K2EDTA and stored at 4°C. Genomic DNA was extracted from ticks and blood samples using a QIAamp DNA mini kit and a QIAamp DNA blood mini kit (Qiagen) respectively. DNA samples were analyzed by polymerase chain reaction (PCR) for the presence of <i>B. burgdorferi</i> using oligonucleotide primers specific for the OspA gene.
A total of 976 ticks were collected. Three different species were obtained from raccoons; <i>Dermacentor variabilis, Amblyomma americanum</i>, and <i>Ixodes sp</i>. <i>Dermacentor variabilis</i> was the only
tick species found on opossums. Twenty-five percent (163/642) of the
tick DNA samples were positive for <i>Borrelia burgdorferi</i>. Prevalence of <i>B. burgdorferi</i> by
tick species was 24.4% (141/577) in <i>D. variabilis</i>, 40.6% (13/32) in <i>A. americanum</i>, and 27.6% (8/29) in <i>I. scapularis</i>. In the present study, 15.7% (8/51) of the total raccoon blood samples examined by PCR were positive for <i>B. burgdorferi</i>, while no opossum blood samples were positive. The high prevalence of <i>B. burgdorferi</i> in ticks common to raccoons and opossums observed in this study, as well as in a
tick species that aggressively bites humans in the southeast U. S. (<i>A. americanum</i>), creates concern that there are ample opportunities for people to come in contact with the infected ticks on these animals. Future studies are urgently needed to fully assess the presence and prevalence of <i>B. burgdorferi</i> in Kentucky and other southeastern states in the U. S.
Advisors/Committee Members: Dr. Cheryl Davis (Director), Dr. Nancy Rice, Dr. Rodney King.
Subjects/Keywords: lyme disease; public health; tick-Borne pathogens; genome sequence; Cell Biology; Genetics; Public Health
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APA ·
Chicago ·
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APA (6th Edition):
Tackett, K. (2009). The Lyme Disease Spirochete, <i>Borrelia burgdorferi</i>, in Tick Species Collected from Raccoons (<i>Procyon lotor</i>) and Opossums (<i>Didelphis virginiana</i>) Trapped in the Warren and Barren Counties of South Central Kentucky. (Masters Thesis). Western Kentucky University. Retrieved from https://digitalcommons.wku.edu/theses/118
Chicago Manual of Style (16th Edition):
Tackett, Kristina. “The Lyme Disease Spirochete, <i>Borrelia burgdorferi</i>, in Tick Species Collected from Raccoons (<i>Procyon lotor</i>) and Opossums (<i>Didelphis virginiana</i>) Trapped in the Warren and Barren Counties of South Central Kentucky.” 2009. Masters Thesis, Western Kentucky University. Accessed January 22, 2021.
https://digitalcommons.wku.edu/theses/118.
MLA Handbook (7th Edition):
Tackett, Kristina. “The Lyme Disease Spirochete, <i>Borrelia burgdorferi</i>, in Tick Species Collected from Raccoons (<i>Procyon lotor</i>) and Opossums (<i>Didelphis virginiana</i>) Trapped in the Warren and Barren Counties of South Central Kentucky.” 2009. Web. 22 Jan 2021.
Vancouver:
Tackett K. The Lyme Disease Spirochete, <i>Borrelia burgdorferi</i>, in Tick Species Collected from Raccoons (<i>Procyon lotor</i>) and Opossums (<i>Didelphis virginiana</i>) Trapped in the Warren and Barren Counties of South Central Kentucky. [Internet] [Masters thesis]. Western Kentucky University; 2009. [cited 2021 Jan 22].
Available from: https://digitalcommons.wku.edu/theses/118.
Council of Science Editors:
Tackett K. The Lyme Disease Spirochete, <i>Borrelia burgdorferi</i>, in Tick Species Collected from Raccoons (<i>Procyon lotor</i>) and Opossums (<i>Didelphis virginiana</i>) Trapped in the Warren and Barren Counties of South Central Kentucky. [Masters Thesis]. Western Kentucky University; 2009. Available from: https://digitalcommons.wku.edu/theses/118
Freie Universität Berlin
13.
Maaz, Denny.
Der Einfluss von Koinfektionen auf die Reservoirkompetenz von peridomestischen Nagetieren für Zecken-übertragene Krankheitserreger.
Degree: 2019, Freie Universität Berlin
URL: http://dx.doi.org/10.17169/refubium-1570
► Kleine Nagetiere und ihre Nester sind faszinierende und existentielle Biotope für zahlreiche Arthropoden-, Helminthen-, und intestinale Protozoenarten. Auf der anderen Seite können einige der parasitischen…
(more)
▼ Kleine Nagetiere und ihre Nester sind faszinierende und existentielle Biotope für zahlreiche Arthropoden-, Helminthen-, und intestinale Protozoenarten. Auf der anderen Seite können einige der parasitischen Arten Krankheiten bei Menschen und Haustieren verursachen. Zudem sind wilde Wühlmäuse und Mäuse Reservoirwirte für viele Zecken übertragene Pathogene. Trotz allem sind die Biodiversität und das quantitative Vorkommen vieler Wildnagerparasiten, sowie ihr zoonotisches Potential noch immer weitgehend unbekannt.
Sechs Nagetierarten aus den Gattungen Apodemus, Myodes und Microtus umfassen wahrscheinlich die individuenreichsten, nicht kommensalen Nagetierarten in Europa und gehören zugleich zu den wichtigsten Wirten für juvenile Zeckenstadien in urbanen Gebieten wie Berlin. Daher fasst diese Thesis zu Beginn erstmalig alle 460 Invertebraten- und 69 Protozoenarten zusammen, welche diese sechs Nagetierarten in Europa parasitieren oder anderweitig mit ihnen assoziiert sind und gibt zudem einen kurzen Überblick über die Taxonomie, die wichtigsten morphologischen Charakteristiken, die Lebenszyklen und das zoonotische Potential der Makroparasiten und intestinalen Kokzidien.
In einer longitudinalen Freilandstudie wurden 257 Nagetiere dieser sechs Arten an vier Fangorten in Berlin gefangen und auf intestinale Kokzidien, Makroparasiten und andere Invertebraten untersucht. Die Ektoparasiten, sowie phoretische und nicht parasitische Arthropoden, welche im Fell und auf der Haut der Nagetiere gefunden wurden, werden im Manuskript 1 behandelt, während Helminthen und intestinale Kokzidien in Manuskript 2 präsentiert werden. Eine hohe Taxonvielfalt von mindestens 84 Arten, welche sich aus 63 Arthropoden , 20 Helminthenarten und einem höheren Kokzidientaxon zusammensetzt, wurde auf/in den Nagetieren in Berlin festgestellt und kein einziges Tier war frei von parasitischen Infektionen. Die Zecke Ixodes ricinus war die häufigste Art mit einer Prävalenz von 56% und einer mittleren Intensität von 9,4 Zecken pro Nagetierwirt. Acht Milbenarten und eine Nematodenart stellten Erstnachweise für die deutsche Fauna dar. Nagetierart, Fangort und Jahreszeit hatten deutlichen Einfluss auf das quantitative Vorkommen der meisten Parasitengruppen. Mäuse waren häufiger mit Trematoden, hymenolepididen Bandwürmern, dem Nematoden Heterakis spumosa, Tierläusen und listrophoriden Milben, aber seltener mit anoplocephaliden Bandwürmern, trombiculiden und myocoptiden Milben befallen als Wühlmäuse. Unter Verwendung einer Nicht-metrischen multidimensionalen Skalierung erschien die Wirtsart von größerer Bedeutung zu sein als der Fangort, da die Nagetierindividuen allein aufgrund ihrer Parasitentaxa einer von drei Wirtstaxongruppen zugeordnet werden konnten. Der unterschiedliche Grad an Urbanisierung zwischen den Fangorten hingegen beeinflusste nicht nur die Präsenz und Abundanz der Nagetierarten, sondern auch den Artenreichtum der Nagetierparasiten. Im Vergleich zu ländlichen Gebieten waren die Mäuse und Wühlmäuse in Berlin mit weniger Arthropodenarten mit…
Advisors/Committee Members: male (gender), von Samson-Himmelstjerna, Georg (firstReferee), Hofer, Heribert (furtherReferee).
Subjects/Keywords: ticks; wild rodents; tick-borne pathogens; co-infections; ectoparasites; endoparasites; Arthropoda; mites; helminths; Ixodes ricinus; 500 Naturwissenschaften und Mathematik::570 Biowissenschaften; Biologie::570 Biowissenschaften; Biologie; 500 Naturwissenschaften und Mathematik::570 Biowissenschaften; Biologie::577 Ökologie; 500 Naturwissenschaften und Mathematik::590 Tiere (Zoologie)::590 Tiere (Zoologie)
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APA (6th Edition):
Maaz, D. (2019). Der Einfluss von Koinfektionen auf die Reservoirkompetenz von peridomestischen Nagetieren für Zecken-übertragene Krankheitserreger. (Thesis). Freie Universität Berlin. Retrieved from http://dx.doi.org/10.17169/refubium-1570
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Maaz, Denny. “Der Einfluss von Koinfektionen auf die Reservoirkompetenz von peridomestischen Nagetieren für Zecken-übertragene Krankheitserreger.” 2019. Thesis, Freie Universität Berlin. Accessed January 22, 2021.
http://dx.doi.org/10.17169/refubium-1570.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Maaz, Denny. “Der Einfluss von Koinfektionen auf die Reservoirkompetenz von peridomestischen Nagetieren für Zecken-übertragene Krankheitserreger.” 2019. Web. 22 Jan 2021.
Vancouver:
Maaz D. Der Einfluss von Koinfektionen auf die Reservoirkompetenz von peridomestischen Nagetieren für Zecken-übertragene Krankheitserreger. [Internet] [Thesis]. Freie Universität Berlin; 2019. [cited 2021 Jan 22].
Available from: http://dx.doi.org/10.17169/refubium-1570.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Maaz D. Der Einfluss von Koinfektionen auf die Reservoirkompetenz von peridomestischen Nagetieren für Zecken-übertragene Krankheitserreger. [Thesis]. Freie Universität Berlin; 2019. Available from: http://dx.doi.org/10.17169/refubium-1570
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
14.
Agostinho Gonçalves Costa da Silva, Patrícia.
Functions and requirements of conserved RNA structures in the 3’ untranslated region of Flaviviruses.
Degree: 2011, Department of Medical Microbiology, Faculty of Medicine / Leiden university Medical Center (LUMC), Leiden University
URL: http://hdl.handle.net/1887/17775
► The Flavivirus genus contains nearly 80 viruses, including many important human pathogens such as dengue virus, yellow fever virus, West Nile virus and tick-borne encephalitis…
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▼ The Flavivirus genus contains nearly 80 viruses, including many important human pathogens such as dengue virus, yellow fever virus, West Nile virus and tick-borne encephalitis virus. To reduce and prevent the impact of flavivirus infection on society, vaccines and effective therapies are required. However, this can only be achieved by increasing our knowledge regarding fundamental aspects of the molecular biology of flaviviruses and a better understanding of the interactions between the virus, the host and the vector. Flaviviruses are small, enveloped viruses containing a positive single-stranded RNA genome of approximately 11 kb with a 5’-cap structure and a non-polyadenylated 3’ end. The flavivirus 3’ untranslated region (UTR) is known to be important for replication and translation of the viral genome. The 3’ UTR contains well conserved RNA sequences and is predicted to fold into a highly complex structure involving several stem-loops and RNA pseudoknots. Some of these motifs and structures have been studied in detail and attributed a biological function, although only a small fraction of the predicted structures has been confirmed by probing. The aim of this thesis was to characterize and determine the biological function of RNA elements present in the flavivirus 3’ UTR.
Subjects/Keywords: Dengue virus; Flavivirus genus; Flavivirus infection; Pathogens; RNA; Tick-borne encephalitis virus; Vaccines; Viruses; West Nile virus; Yellow fever virus
…arthropod-borne and many of them are important human
pathogens that can cause a variety of… …virus (JEV), West Nile virus (WNV), and tick-borne
encephalitis virus… …x29; mosquito-borne, (ii) tick-borne, and (iii) no known
vector (… …established that mosquito- and tick-borne viruses represent two different evolutionary lineages 20… …borne viruses later diverged into the tick-borne and then
the mosquito-borne virus clusters…
Record Details
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Record Details
Similar Records
Cite
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❌
APA ·
Chicago ·
MLA ·
Vancouver ·
CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Agostinho Gonçalves Costa da Silva, P. (2011). Functions and requirements of conserved RNA structures in the 3’ untranslated region of Flaviviruses. (Doctoral Dissertation). Department of Medical Microbiology, Faculty of Medicine / Leiden university Medical Center (LUMC), Leiden University. Retrieved from http://hdl.handle.net/1887/17775
Chicago Manual of Style (16th Edition):
Agostinho Gonçalves Costa da Silva, Patrícia. “Functions and requirements of conserved RNA structures in the 3’ untranslated region of Flaviviruses.” 2011. Doctoral Dissertation, Department of Medical Microbiology, Faculty of Medicine / Leiden university Medical Center (LUMC), Leiden University. Accessed January 22, 2021.
http://hdl.handle.net/1887/17775.
MLA Handbook (7th Edition):
Agostinho Gonçalves Costa da Silva, Patrícia. “Functions and requirements of conserved RNA structures in the 3’ untranslated region of Flaviviruses.” 2011. Web. 22 Jan 2021.
Vancouver:
Agostinho Gonçalves Costa da Silva P. Functions and requirements of conserved RNA structures in the 3’ untranslated region of Flaviviruses. [Internet] [Doctoral dissertation]. Department of Medical Microbiology, Faculty of Medicine / Leiden university Medical Center (LUMC), Leiden University; 2011. [cited 2021 Jan 22].
Available from: http://hdl.handle.net/1887/17775.
Council of Science Editors:
Agostinho Gonçalves Costa da Silva P. Functions and requirements of conserved RNA structures in the 3’ untranslated region of Flaviviruses. [Doctoral Dissertation]. Department of Medical Microbiology, Faculty of Medicine / Leiden university Medical Center (LUMC), Leiden University; 2011. Available from: http://hdl.handle.net/1887/17775
. 
Open Access Theses and Dissertations
