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1. Addae-Mensah, Kweku Amissah. A microfabricated microcantilever array: A platform for investigation of cellular biomechanics and microforces in vitro.

Degree: PhD, Biomedical Engineering, 2008, Vanderbilt University

Living cells and tissues experience mechanical forces in their physiological environments that are known to affect many cellular processes. Also of importance are the mechanical properties of cells, as well as the microforces generated by cellular processes in their microenvironments. The difficulty associated with studying these phenomena in vivo has led to alternatives such as using in vitro models. The need for experimental techniques for investigating cellular biomechanics and mechanobiology in vitro has fueled an evolution in the technology used in these studies. Particularly noteworthy are some of the new biomicroelectromechanical systems (BioMEMs) devices and techniques. This study describes cellular micromechanical techniques and methods that have been developed for extit{in vitro} studies. Improvements made to a passive array of vertical microcantilevers, for detecting cellular microforces and studying in vitro cell mechanics are presented. A new technique that uses poly(vinyl alcohol) (PVA) as a lift-off agent to attach structures to the microcantilevers, thereby providing a means to actively move the microcantilevers is introduced. The use of the improved microcantilever array platform as a potential assay for cardiac myofibrillogenesis will also be described. Finally the use of cryogenic etching techniques for making master molds for the microcantilever arrays is described, and subsequent arrays are used to investigate the biological responses of mesenchymal stem cells to forces generated by post deflections. Advisors/Committee Members: Kevin K. Parker (committee member), Franz J. Baudenbacher (committee member), Paul E. Moore (committee member), Michael I. Miga (committee member), John P. Wikswo (chair).

Subjects/Keywords: microcontact printing labeling techniques; quantum dots; mesengenic process; SU-8; PDMS; polydimethylsiloxane; salinization; stress fiber like structures; SFLS; fluorescene recovery after photobleaching; FRAP; subcelluar laser ablation; SLA; hippocampal neurons; two dimensional fast fourier transform; 2D-FFT; bandpass filtering; measurement techniques; scotch tape test; blister test; adhesion strength; polymer composite structures; cell-cell adhesion; slow cardiac calcium waves; volumetric strain; volumetric distortion; divergence; curl; Otsu threshold; dry reactive ion etching; DRIE; reactive ion etching; RIE; softh lithography; projection lithography; contact lithography; e-beam lithography; HASM; human airway smooth muscle

laser ablation. UV Ultra violet. VIIBRE Vanderbilt Institute for Integrative Biosystems… …used to tug and pull, creating linear forces. A laser beam is used in tandem with a… …is functionalized, is bound to the cell surface as in MTC. The high intensity laser beam… 

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6th Edition):

Addae-Mensah, K. A. (2008). A microfabricated microcantilever array: A platform for investigation of cellular biomechanics and microforces in vitro. (Doctoral Dissertation). Vanderbilt University. Retrieved from http://etd.library.vanderbilt.edu//available/etd-08182008-153315/ ;

Chicago Manual of Style (16th Edition):

Addae-Mensah, Kweku Amissah. “A microfabricated microcantilever array: A platform for investigation of cellular biomechanics and microforces in vitro.” 2008. Doctoral Dissertation, Vanderbilt University. Accessed October 16, 2019. http://etd.library.vanderbilt.edu//available/etd-08182008-153315/ ;.

MLA Handbook (7th Edition):

Addae-Mensah, Kweku Amissah. “A microfabricated microcantilever array: A platform for investigation of cellular biomechanics and microforces in vitro.” 2008. Web. 16 Oct 2019.

Vancouver:

Addae-Mensah KA. A microfabricated microcantilever array: A platform for investigation of cellular biomechanics and microforces in vitro. [Internet] [Doctoral dissertation]. Vanderbilt University; 2008. [cited 2019 Oct 16]. Available from: http://etd.library.vanderbilt.edu//available/etd-08182008-153315/ ;.

Council of Science Editors:

Addae-Mensah KA. A microfabricated microcantilever array: A platform for investigation of cellular biomechanics and microforces in vitro. [Doctoral Dissertation]. Vanderbilt University; 2008. Available from: http://etd.library.vanderbilt.edu//available/etd-08182008-153315/ ;

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