subject:(spindle)

University of Oxford

1. Dunsch, Anja Katrin. Control of the mitotic spindle by dynein light chain 1 complexes.

Degree: PhD, 2013, University of Oxford

URL: http://ora.ox.ac.uk/objects/uuid:b2fd5670-a035-42ca-aaef-78a30aeaa084 ; https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.595940

► Robust control mechanisms ensure faithful inheritance of an intact genome through the processes of mitosis and cytokinesis. Different populations of the cytoplasmic dynein motor deﬁned… (more)

▼ Robust control mechanisms ensure faithful inheritance of an intact genome through the processes of mitosis and cytokinesis. Different populations of the cytoplasmic dynein motor deﬁned by speciﬁc dynein adaptor complexes are required for the formation of a stable bipolar mitotic spindle. This study analysed how different dynein subcomplexes contribute to spindle formation and orientation. Various dynein subpopulations were identiﬁed by mass spectrometry. I have shown that the dynein light chain 1 (DYNLL1) directly interacts with the kinetochore localised Astrin-Kinastrin complex as well as the spindle microtubule associated complex formed by CHICA and HMMR. I have characterised both complexes and identiﬁed unique functions in chromosome alignment and mitotic spindle orientation, respectively. I have found that Kinastrin (C15orf23) is the major Astrin-interacting protein in mitotic cells and is required for Astrin targeting to microtubule plus ends proximal to the plus tip tracking protein EB1. Fixed cell microscopy revealed that cells over-expressing or depleted of Kinastrin mislocalise Astrin. Additionally, depletion of the Astrin-Kinastrin complex delays chromosome alignment and causes the loss of normal spindle architecture and sister chromatid cohesion before anaphase onset (Dunsch et al., 2011). Using immunoprecipitation and microtubule binding assays, I have shown that CHICA and HMMR interact with one another, and target to the spindle by a microtubule-binding site in the amino-terminal region of HMMR. CHICA interacts with DYNLL1 by a series of conserved TQT motifs in the carboxy-terminal region. Depletion of DYNLL1, CHICA or HMMR causes a slight increase in mitotic index but has little effect on spindle formation or checkpoint function. Fixed and live cell microscopy reveal, however, that the asymmetric distribution of cor tical dynein is lost and the spindle in these cells fails to orient correctly in relation to the culture surface (Dunsch et al., 2012). These ﬁndings presented here suggest that the Astrin-Kinastrin complex is required for normal spindle architecture and chromosome alignment, and that per turbations of this pathway result in delayed mitosis and non-physiological separase activation, whereas HMMR and CHICA act as par t of a dynein-DYNLL1 complex with a speciﬁc function deﬁning or controlling spindle orientation.

Subjects/Keywords: 571.8; Cell Biology; Mitotic spindle; Spindle orientation

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APA (6^th Edition):

Dunsch, A. K. (2013). Control of the mitotic spindle by dynein light chain 1 complexes. (Doctoral Dissertation). University of Oxford. Retrieved from http://ora.ox.ac.uk/objects/uuid:b2fd5670-a035-42ca-aaef-78a30aeaa084 ; https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.595940

Chicago Manual of Style (16^th Edition):

Dunsch, Anja Katrin. “Control of the mitotic spindle by dynein light chain 1 complexes.” 2013. Doctoral Dissertation, University of Oxford. Accessed January 17, 2021. http://ora.ox.ac.uk/objects/uuid:b2fd5670-a035-42ca-aaef-78a30aeaa084 ; https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.595940.

MLA Handbook (7^th Edition):

Dunsch, Anja Katrin. “Control of the mitotic spindle by dynein light chain 1 complexes.” 2013. Web. 17 Jan 2021.

Vancouver:

Dunsch AK. Control of the mitotic spindle by dynein light chain 1 complexes. [Internet] [Doctoral dissertation]. University of Oxford; 2013. [cited 2021 Jan 17]. Available from: http://ora.ox.ac.uk/objects/uuid:b2fd5670-a035-42ca-aaef-78a30aeaa084 ; https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.595940.

Council of Science Editors:

Dunsch AK. Control of the mitotic spindle by dynein light chain 1 complexes. [Doctoral Dissertation]. University of Oxford; 2013. Available from: http://ora.ox.ac.uk/objects/uuid:b2fd5670-a035-42ca-aaef-78a30aeaa084 ; https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.595940

Brno University of Technology

2. Huňka, Radek. Výměna nástrojů u svislého soustruhu: Tool changing of vertical turning lathe.

Degree: 2018, Brno University of Technology

URL: http://hdl.handle.net/11012/4583

► This master's thesis deals with the problems of tool changing of vertical turning lathe. Specifically about modular system of spindle locking in a ram. The… (more)

Subjects/Keywords: Vřeteno; smykadlo; aretace; Spindle; spindle ram; locking

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APA (6^th Edition):

Huňka, R. (2018). Výměna nástrojů u svislého soustruhu: Tool changing of vertical turning lathe. (Thesis). Brno University of Technology. Retrieved from http://hdl.handle.net/11012/4583

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Huňka, Radek. “Výměna nástrojů u svislého soustruhu: Tool changing of vertical turning lathe.” 2018. Thesis, Brno University of Technology. Accessed January 17, 2021. http://hdl.handle.net/11012/4583.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Huňka, Radek. “Výměna nástrojů u svislého soustruhu: Tool changing of vertical turning lathe.” 2018. Web. 17 Jan 2021.

Vancouver:

Huňka R. Výměna nástrojů u svislého soustruhu: Tool changing of vertical turning lathe. [Internet] [Thesis]. Brno University of Technology; 2018. [cited 2021 Jan 17]. Available from: http://hdl.handle.net/11012/4583.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Huňka R. Výměna nástrojů u svislého soustruhu: Tool changing of vertical turning lathe. [Thesis]. Brno University of Technology; 2018. Available from: http://hdl.handle.net/11012/4583

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Rutgers University

3. Wang, Lin-Ing. Regulation of sister centromere fusion and meiotic spindle assembly in Drosophila oocytes.

Degree: PhD, Cell and Developmental Biology, 2019, Rutgers University

URL: https://rucore.libraries.rutgers.edu/rutgers-lib/61996/

►

Accurate meiotic chromosome segregation prevents aneuploidy that can cause developmental problems and lead to infertility. The majority of errors causing aneuploidy in gametes are maternal… (more)

▼

Accurate meiotic chromosome segregation prevents aneuploidy that can cause developmental problems and lead to infertility. The majority of errors causing aneuploidy in gametes are maternal in origin due to several innate differences in female meiosis. In contrast to mitosis and male meiosis, bi-polar spindles in female meiosis are assembled in the absence of centrosomes. Instead, the chromosomes direct bi-polar spindle assembly. Abnormalities in spindle formation or structure can cause non-disjunction of the chromosomes. To understand the mechanisms of proper chromosome segregation in female meiosis, I focused on sister centromere cohesion and spindle assembly in Drosophila oocytes. One important mechanism for proper chromosome segregation in meiosis is that the sister chromatids co-orient via sister centromere fusion, resulting in microtubule attachment to a shared spindle pole. This phenomenon is unique to meiosis I, and is well-studied in budding and fission yeast, it remains unclear how it is regulated in other organisms. Previously our lab has identified two proteins that are required for sister centromere fusion. I characterized these two genes and surprisingly found they regulate sister centromere fusion by two different mechanisms. SPC105R, a kinetochore protein, regulates centromere fusion by recruiting proteins that protect cohesins. Protein phosphatase 1 (PP1), on the other hand, regulates this phenomenon through antagonizing proteins that stabilize microtubule attachment. The latter result suggests that stable microtubule attachment in metaphase I is the prerequisite to release sister centromere fusion without cohesin removal before metaphase II. The chromosomal passenger complex (CPC) has four subunits (INCENP, Aurora B, Borealin and Survivin/Deterin) and it is essential for spindle assembly in Drosophila oocytes. I made several separation-of-function mutations within the Incenp subunit of the CPC and found that interactions with Heterochromatin Protein 1 (HP1) are key to direct CPC-dependent spindle assembly in oocytes. HP1 firstly recruits the CPC to chromosomes through interacting with Borealin, and this interaction is also required for building the central spindle, the central overlap region of the microtubules. Additionally, I found evidence that HP1 interacts directly with INCENP within the central spindle in oocytes to promote homologous chromosome bi-orientation. These results give rise to a model where HP1 could be a novel CPC targeting subunit and reveals a mechanism for how the CPC mediates chromosome-mediated spindle assembly.

Advisors/Committee Members: Mckim, Kim (chair), Steward, Ruth (internal member), Schindler, Karen (internal member), Walworth, Nancy (outside member), School of Graduate Studies.

Subjects/Keywords: Spindle assembly; Drosophila – Genetics; Spindle (Cell division)

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APA (6^th Edition):

Wang, L. (2019). Regulation of sister centromere fusion and meiotic spindle assembly in Drosophila oocytes. (Doctoral Dissertation). Rutgers University. Retrieved from https://rucore.libraries.rutgers.edu/rutgers-lib/61996/

Chicago Manual of Style (16^th Edition):

Wang, Lin-Ing. “Regulation of sister centromere fusion and meiotic spindle assembly in Drosophila oocytes.” 2019. Doctoral Dissertation, Rutgers University. Accessed January 17, 2021. https://rucore.libraries.rutgers.edu/rutgers-lib/61996/.

MLA Handbook (7^th Edition):

Wang, Lin-Ing. “Regulation of sister centromere fusion and meiotic spindle assembly in Drosophila oocytes.” 2019. Web. 17 Jan 2021.

Vancouver:

Wang L. Regulation of sister centromere fusion and meiotic spindle assembly in Drosophila oocytes. [Internet] [Doctoral dissertation]. Rutgers University; 2019. [cited 2021 Jan 17]. Available from: https://rucore.libraries.rutgers.edu/rutgers-lib/61996/.

Council of Science Editors:

Wang L. Regulation of sister centromere fusion and meiotic spindle assembly in Drosophila oocytes. [Doctoral Dissertation]. Rutgers University; 2019. Available from: https://rucore.libraries.rutgers.edu/rutgers-lib/61996/

University of Edinburgh

4. Yuan, Ivan. Generation of synthetic spindle checkpoint signals.

Degree: PhD, 2016, University of Edinburgh

URL: http://hdl.handle.net/1842/22030

► The spindle checkpoint ensures proper chromosome segregation by monitoring kinetochore-microtubule interactions: unattached kinetochores recruit checkpoint proteins that combine to form a diffusible inhibitor which delays… (more)

Subjects/Keywords: 571.8; spindle checkpoint

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APA (6^th Edition):

Yuan, I. (2016). Generation of synthetic spindle checkpoint signals. (Doctoral Dissertation). University of Edinburgh. Retrieved from http://hdl.handle.net/1842/22030

Chicago Manual of Style (16^th Edition):

Yuan, Ivan. “Generation of synthetic spindle checkpoint signals.” 2016. Doctoral Dissertation, University of Edinburgh. Accessed January 17, 2021. http://hdl.handle.net/1842/22030.

MLA Handbook (7^th Edition):

Yuan, Ivan. “Generation of synthetic spindle checkpoint signals.” 2016. Web. 17 Jan 2021.

Vancouver:

Yuan I. Generation of synthetic spindle checkpoint signals. [Internet] [Doctoral dissertation]. University of Edinburgh; 2016. [cited 2021 Jan 17]. Available from: http://hdl.handle.net/1842/22030.

Council of Science Editors:

Yuan I. Generation of synthetic spindle checkpoint signals. [Doctoral Dissertation]. University of Edinburgh; 2016. Available from: http://hdl.handle.net/1842/22030

Brno University of Technology

5. Petrych, Jan. Návrh přídavného frézovacího zařízení pro CNC Masturn MT54: Design of additional milling equipment for CNC Masturn MT54.

Degree: 2019, Brno University of Technology

URL: http://hdl.handle.net/11012/66290

► This bachelor thesis deals with constructional design of milling spindle for CNC cutter MASTRUN MT54. First part contains thorough analysis of milling spindle problematics. Second… (more)

Subjects/Keywords: Vřeteno; vřetenová ložiska; konstrukce vřetene; pohon vřetene; Spindle; spindle bearings; spindle design; spindle drive

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APA (6^th Edition):

Petrych, J. (2019). Návrh přídavného frézovacího zařízení pro CNC Masturn MT54: Design of additional milling equipment for CNC Masturn MT54. (Thesis). Brno University of Technology. Retrieved from http://hdl.handle.net/11012/66290

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Petrych, Jan. “Návrh přídavného frézovacího zařízení pro CNC Masturn MT54: Design of additional milling equipment for CNC Masturn MT54.” 2019. Thesis, Brno University of Technology. Accessed January 17, 2021. http://hdl.handle.net/11012/66290.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Petrych, Jan. “Návrh přídavného frézovacího zařízení pro CNC Masturn MT54: Design of additional milling equipment for CNC Masturn MT54.” 2019. Web. 17 Jan 2021.

Vancouver:

Petrych J. Návrh přídavného frézovacího zařízení pro CNC Masturn MT54: Design of additional milling equipment for CNC Masturn MT54. [Internet] [Thesis]. Brno University of Technology; 2019. [cited 2021 Jan 17]. Available from: http://hdl.handle.net/11012/66290.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Petrych J. Návrh přídavného frézovacího zařízení pro CNC Masturn MT54: Design of additional milling equipment for CNC Masturn MT54. [Thesis]. Brno University of Technology; 2019. Available from: http://hdl.handle.net/11012/66290

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Brno University of Technology

6. Voda, Pavel. Konstrukce frézovacího vřetene: Design of milling spindl.

Degree: 2019, Brno University of Technology

URL: http://hdl.handle.net/11012/3431

► The aim of this thesis called Design of milling spindl is research and description of milling spindles. The first part is focused on general description… (more)

Subjects/Keywords: Vřeteno; konstrukce vřetena; vřetenová ložiska; pohon vřetena; Spindle; spindle design; spindle bearings; spindle drive

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APA (6^th Edition):

Voda, P. (2019). Konstrukce frézovacího vřetene: Design of milling spindl. (Thesis). Brno University of Technology. Retrieved from http://hdl.handle.net/11012/3431

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Voda, Pavel. “Konstrukce frézovacího vřetene: Design of milling spindl.” 2019. Thesis, Brno University of Technology. Accessed January 17, 2021. http://hdl.handle.net/11012/3431.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Voda, Pavel. “Konstrukce frézovacího vřetene: Design of milling spindl.” 2019. Web. 17 Jan 2021.

Vancouver:

Voda P. Konstrukce frézovacího vřetene: Design of milling spindl. [Internet] [Thesis]. Brno University of Technology; 2019. [cited 2021 Jan 17]. Available from: http://hdl.handle.net/11012/3431.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Voda P. Konstrukce frézovacího vřetene: Design of milling spindl. [Thesis]. Brno University of Technology; 2019. Available from: http://hdl.handle.net/11012/3431

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Brno University of Technology

7. Novák, Tomáš. Konstrukce vřetene soustruhu: Design of lathe spindle.

Degree: 2018, Brno University of Technology

URL: http://hdl.handle.net/11012/10707

► The subject of this bachelor's thesis is a literary overview and a simple design of a lathe spindle with basic calculations. The first part contains… (more)

Subjects/Keywords: Soustruh; vřeteno; konstrukce vřetene; vřetenová ložiska; parametry vřetene; pohon vřetene.; Lathe; spindle; spindle design; spindle bearings; spindle characteristics; spindle drive.

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APA (6^th Edition):

Novák, T. (2018). Konstrukce vřetene soustruhu: Design of lathe spindle. (Thesis). Brno University of Technology. Retrieved from http://hdl.handle.net/11012/10707

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Novák, Tomáš. “Konstrukce vřetene soustruhu: Design of lathe spindle.” 2018. Thesis, Brno University of Technology. Accessed January 17, 2021. http://hdl.handle.net/11012/10707.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Novák, Tomáš. “Konstrukce vřetene soustruhu: Design of lathe spindle.” 2018. Web. 17 Jan 2021.

Vancouver:

Novák T. Konstrukce vřetene soustruhu: Design of lathe spindle. [Internet] [Thesis]. Brno University of Technology; 2018. [cited 2021 Jan 17]. Available from: http://hdl.handle.net/11012/10707.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Novák T. Konstrukce vřetene soustruhu: Design of lathe spindle. [Thesis]. Brno University of Technology; 2018. Available from: http://hdl.handle.net/11012/10707

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of California – Berkeley

8. Woodruff, Jeffrey Blake. Mechanisms of Mitotic Spindle Disassembly and Positioning in Saccharomyces cerevisiae.

Degree: Molecular & Cell Biology, 2011, University of California – Berkeley

URL: http://www.escholarship.org/uc/item/923199gx

► When a cell divides, it must accurately replicate its genetic material and then faithfully segregate this material into the resulting daughter cells. My research addresses… (more)

▼ When a cell divides, it must accurately replicate its genetic material and then faithfully segregate this material into the resulting daughter cells. My research addresses the latter half of this problem, focusing on how the cell regulates the function of the mitotic spindle, an elegant microtubule-based machine that attaches to replicated DNA and pulls it apart during mitosis. Here, I present two studies that investigate how the cell disassembles the mitotic spindle at the end of mitosis and how the cell positions the mitotic spindle prior to mitotic completion.I combined genetic analysis with live-cell fluorescence microscopy to identify the subprocesses driving spindle disassembly as well as the proteins that perform these subprocesses. Our results suggest that mechanistically distinct pathways largely governed by the anaphase-promoting complex, Aurora B kinase, and kinesin-8 cooperate to drive spindle disassembly in budding yeast. We also describe the roles of novel disassembly factors such as the spindle protein She1 and the 7-protein Alternative Replication Factor C complex. Together, these pathways disengage the mitotic spindle halves, inhibit spindle microtubule growth, and promote sustained spindle microtubule depolymerization. Strikingly, combined inhibition of pairs of disassembly pathways yielded cells with hyper-stable spindle remnants, which caused dramatic defects in cell cycle progression, thus establishing that regulated and rapid spindle disassembly is crucial for cell proliferation.To better understand the mechanisms of spindle positioning, I examined how the dynein-driven spindle-positioning pathway in budding yeast is silenced. My work suggests that dynein activity is regulated through interaction with the multi-subunit dynactin complex at anaphase and identifies a new cellular factor, She1, which controls this interaction. Dynactin is a well-known dynein activator, and, in budding yeast, the complete complex is required for dynein-dependent spindle movement. I found that localization of the dynactin complex is cell cycle-regulated, such that dynactin is recruited to astral microtubules, via interaction with dynein, primarily during anaphase. Additionally, we discovered that the protein She1 is a cell cycle-regulated inhibitor of dynein activity. Without She1, dynein activity extends beyond anaphase and, as a result, mis-positions the mitotic spindle. Strikingly, loss of She1 also permits recruitment of the dynactin complex to astral microtubules throughout the cell cycle. These results suggest that in wild-type cells, She1 restricts dynein activity to anaphase by preventing the interaction between dynein and the complete dynactin complex.

Subjects/Keywords: Biology; Cellular biology; Aurora B; Mitosis; She1; Spindle; Spindle disassembly; Spindle positioning

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APA (6^th Edition):

Woodruff, J. B. (2011). Mechanisms of Mitotic Spindle Disassembly and Positioning in Saccharomyces cerevisiae. (Thesis). University of California – Berkeley. Retrieved from http://www.escholarship.org/uc/item/923199gx

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Woodruff, Jeffrey Blake. “Mechanisms of Mitotic Spindle Disassembly and Positioning in Saccharomyces cerevisiae.” 2011. Thesis, University of California – Berkeley. Accessed January 17, 2021. http://www.escholarship.org/uc/item/923199gx.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Woodruff, Jeffrey Blake. “Mechanisms of Mitotic Spindle Disassembly and Positioning in Saccharomyces cerevisiae.” 2011. Web. 17 Jan 2021.

Vancouver:

Woodruff JB. Mechanisms of Mitotic Spindle Disassembly and Positioning in Saccharomyces cerevisiae. [Internet] [Thesis]. University of California – Berkeley; 2011. [cited 2021 Jan 17]. Available from: http://www.escholarship.org/uc/item/923199gx.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Woodruff JB. Mechanisms of Mitotic Spindle Disassembly and Positioning in Saccharomyces cerevisiae. [Thesis]. University of California – Berkeley; 2011. Available from: http://www.escholarship.org/uc/item/923199gx

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Edinburgh

9. Costa, Mariana Fernandes Alves. Molecular remodelling of the spindle architecture during metaphase arrest in oocytes.

Degree: PhD, 2018, University of Edinburgh

URL: http://hdl.handle.net/1842/31255

► Oocytes of most species assemble and maintain a functional bipolar spindle in the absence of centrosomes. Strikingly, after bipolar spindle formation, oocytes arrest in metaphase… (more)

▼ Oocytes of most species assemble and maintain a functional bipolar spindle in the absence of centrosomes. Strikingly, after bipolar spindle formation, oocytes arrest in metaphase for several hours before fertilisation. How the dynamic spindle maintains its bipolarity during this long arrest is poorly understood. I hypothesise that the bipolar spindle is stably maintained by changes in the distribution of microtubule-associated proteins (MAPs) on the spindle during the long oocyte arrest. To test this, I generated transgenic flies expressing GFP-tagged microtubule-associated proteins (MAPs), and found that 13 out of 24 proteins change localisation between early and late oocytes. I refer to these changes in MAP localisation after establishment of bipolarity as 'spindle maturation'. In order to identify the molecular mechanisms triggering MAP relocalisation, I manipulated the kinase activity of the cell cycle regulator Cdk1 by over-expressing non-degradable cyclin A or B, the major activators of Cdk1. Their expression prevented re-localisation of distinct sets of MAPs, and disrupted spindle bipolarity and accurate chromosome segregation in oocytes. Kinesin-6 Pavarotti/MKlp1 localised strongly to the spindle equator in late oocytes, whilst nearly always absent from this region in early oocytes. The localisation of Pavarotti to the spindle equator in late oocytes was reduced when cyclin B is over-expressed in oocytes, suggesting a role for Cdk1/cyclin B complex in regulating Pavarotti localisation. Indeed, a Pavarotti/Mklp1 mutant non-phosphorylatable by Cdk1 prematurely localised to the meiotic spindle and disrupted spindle bipolarity. Moreover, removal of Pavarotti from the metaphase-I spindle by RNAi induced spindle defects in oocytes. Therefore, it is likely that the microtubule cross-linking activity of Pavarotti enhances the stability of the metaphase-I spindle during the long arrest. Consistent with this, I found that the microtubule density in the spindle equator is higher in late oocytes. Altogether, I propose that remodelling the molecular architecture of the spindle during the long oocyte arrest is important to stabilise the bipolar spindle without centrosomes.

Subjects/Keywords: 612.6; spindle maturation; oocytes; bipolar spindle formation; microtubule-associated proteins; MAP relocalisation; spindle bipolarity

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APA (6^th Edition):

Costa, M. F. A. (2018). Molecular remodelling of the spindle architecture during metaphase arrest in oocytes. (Doctoral Dissertation). University of Edinburgh. Retrieved from http://hdl.handle.net/1842/31255

Chicago Manual of Style (16^th Edition):

Costa, Mariana Fernandes Alves. “Molecular remodelling of the spindle architecture during metaphase arrest in oocytes.” 2018. Doctoral Dissertation, University of Edinburgh. Accessed January 17, 2021. http://hdl.handle.net/1842/31255.

MLA Handbook (7^th Edition):

Costa, Mariana Fernandes Alves. “Molecular remodelling of the spindle architecture during metaphase arrest in oocytes.” 2018. Web. 17 Jan 2021.

Vancouver:

Costa MFA. Molecular remodelling of the spindle architecture during metaphase arrest in oocytes. [Internet] [Doctoral dissertation]. University of Edinburgh; 2018. [cited 2021 Jan 17]. Available from: http://hdl.handle.net/1842/31255.

Council of Science Editors:

Costa MFA. Molecular remodelling of the spindle architecture during metaphase arrest in oocytes. [Doctoral Dissertation]. University of Edinburgh; 2018. Available from: http://hdl.handle.net/1842/31255

University of Helsinki

10. Halonen, Risto. The Associations Between Sleep Spindles and Reasoning Abilities in Adolescent Girls and Boys.

Degree: Medicinska fakulteten, 2017, University of Helsinki

URL: http://hdl.handle.net/10138/177289

► Sleep spindles are thalamocortical oscillations that occupy the sigma band with trait-like inter-individual variability. Sleep spindles associate with reasoning abilities according to several studies, but… (more)

▼ Sleep spindles are thalamocortical oscillations that occupy the sigma band with trait-like inter-individual variability. Sleep spindles associate with reasoning abilities according to several studies, but some discrepancy exists in the strength and even direction of the associations. This may, to some extent, be due to methodological differences. The stage of brain maturation also affects spindle manifestation. In this community-based study, associations between spindle characteristics and reasoning abilities are examined in an understudied age group, adolescents. An all-night polysomnography was conducted at homes of 178 adolescents (104 girls). Working memory, visuospatial reasoning and verbal reasoning were measured in the same evening. An automatic algorithm was used to detect slow (10–13 Hz) and fast (13–16 Hz) spindles in frontal and central scalp derivations in NREM 2 sleep stage. The associations between spindle variables (density and intensity) and the cognitive test scores were analyzed with linear regression. Genders apart, the analyses were conducted first on the whole group and then separately on the Above Median (AM) and Below Median (BM) intelligence subgroups. In the analyses with all subjects, higher central fast density associated with better verbal reasoning in girls. When examining the subgroups separately, this association was not perceived in the AM group but appeared prominently in the BM group girls. No other associations were found between the spindle variables and the cognitive test scores. A positive spindle-intelligence relation is an established finding in females, but more commonly the association is typified by fluid/visuospatial reasoning and frontal brain areas. In the present study, young age may have related to the accentuated relative significance of more caudal brain regions and verbal intelligence in relation to spindles. The ongoing neural maturation and the heterogeneity of the sample may have contributed to the nature of the findings. More adolescent studies are needed to gain understanding of the matter.

Subjects/Keywords: sleep spindle; spindle density; spindle intensity; intelligence; reasoning; adolescent; maturation; electroencephalography; Psychology; Psykologia; Psykologi

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APA (6^th Edition):

Halonen, R. (2017). The Associations Between Sleep Spindles and Reasoning Abilities in Adolescent Girls and Boys. (Masters Thesis). University of Helsinki. Retrieved from http://hdl.handle.net/10138/177289

Chicago Manual of Style (16^th Edition):

Halonen, Risto. “The Associations Between Sleep Spindles and Reasoning Abilities in Adolescent Girls and Boys.” 2017. Masters Thesis, University of Helsinki. Accessed January 17, 2021. http://hdl.handle.net/10138/177289.

MLA Handbook (7^th Edition):

Halonen, Risto. “The Associations Between Sleep Spindles and Reasoning Abilities in Adolescent Girls and Boys.” 2017. Web. 17 Jan 2021.

Vancouver:

Halonen R. The Associations Between Sleep Spindles and Reasoning Abilities in Adolescent Girls and Boys. [Internet] [Masters thesis]. University of Helsinki; 2017. [cited 2021 Jan 17]. Available from: http://hdl.handle.net/10138/177289.

Council of Science Editors:

Halonen R. The Associations Between Sleep Spindles and Reasoning Abilities in Adolescent Girls and Boys. [Masters Thesis]. University of Helsinki; 2017. Available from: http://hdl.handle.net/10138/177289

San Jose State University

11. Shamai, Krystle. The Effect of Obesity on Muscle Sensory Neuron Function in Mice.

Degree: MS, Biological Sciences, 2015, San Jose State University

URL: https://doi.org/10.31979/etd.k63d-2esz ; https://scholarworks.sjsu.edu/etd_theses/4667

► The sense of body movement and position in a three dimensional space, known as proprioception, is largely responsible for maintaining proper balance and gait.… (more)

Subjects/Keywords: Mice; Muscle spindle; Obesity; Proprioception

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APA (6^th Edition):

Shamai, K. (2015). The Effect of Obesity on Muscle Sensory Neuron Function in Mice. (Masters Thesis). San Jose State University. Retrieved from https://doi.org/10.31979/etd.k63d-2esz ; https://scholarworks.sjsu.edu/etd_theses/4667

Chicago Manual of Style (16^th Edition):

Shamai, Krystle. “The Effect of Obesity on Muscle Sensory Neuron Function in Mice.” 2015. Masters Thesis, San Jose State University. Accessed January 17, 2021. https://doi.org/10.31979/etd.k63d-2esz ; https://scholarworks.sjsu.edu/etd_theses/4667.

MLA Handbook (7^th Edition):

Shamai, Krystle. “The Effect of Obesity on Muscle Sensory Neuron Function in Mice.” 2015. Web. 17 Jan 2021.

Vancouver:

Shamai K. The Effect of Obesity on Muscle Sensory Neuron Function in Mice. [Internet] [Masters thesis]. San Jose State University; 2015. [cited 2021 Jan 17]. Available from: https://doi.org/10.31979/etd.k63d-2esz ; https://scholarworks.sjsu.edu/etd_theses/4667.

Council of Science Editors:

Shamai K. The Effect of Obesity on Muscle Sensory Neuron Function in Mice. [Masters Thesis]. San Jose State University; 2015. Available from: https://doi.org/10.31979/etd.k63d-2esz ; https://scholarworks.sjsu.edu/etd_theses/4667

Cornell University

12. Bergman, Zane. Excessive Dynein Force In She1 Mutants Reveals Differences In Microtubule Anchorage In Budding Yeast.

Degree: PhD, Biochemistry, 2012, Cornell University

URL: http://hdl.handle.net/1813/29532

► Many microtubule functions, such as chromosome segregation and spindle orientation, depend on a stable anchor point in the cell. In the budding yeast, Saccharomyces cerevisiae,… (more)

Subjects/Keywords: microtubules; tubulin; spindle pole body

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APA (6^th Edition):

Bergman, Z. (2012). Excessive Dynein Force In She1 Mutants Reveals Differences In Microtubule Anchorage In Budding Yeast. (Doctoral Dissertation). Cornell University. Retrieved from http://hdl.handle.net/1813/29532

Chicago Manual of Style (16^th Edition):

Bergman, Zane. “Excessive Dynein Force In She1 Mutants Reveals Differences In Microtubule Anchorage In Budding Yeast.” 2012. Doctoral Dissertation, Cornell University. Accessed January 17, 2021. http://hdl.handle.net/1813/29532.

MLA Handbook (7^th Edition):

Bergman, Zane. “Excessive Dynein Force In She1 Mutants Reveals Differences In Microtubule Anchorage In Budding Yeast.” 2012. Web. 17 Jan 2021.

Vancouver:

Bergman Z. Excessive Dynein Force In She1 Mutants Reveals Differences In Microtubule Anchorage In Budding Yeast. [Internet] [Doctoral dissertation]. Cornell University; 2012. [cited 2021 Jan 17]. Available from: http://hdl.handle.net/1813/29532.

Council of Science Editors:

Bergman Z. Excessive Dynein Force In She1 Mutants Reveals Differences In Microtubule Anchorage In Budding Yeast. [Doctoral Dissertation]. Cornell University; 2012. Available from: http://hdl.handle.net/1813/29532

University of Oregon

13. Lu, Michelle. The Construction and Deconstruction of Signaling Systems that Regulate Mitotic Spindle Positioning.

Degree: PhD, Department of Biology, 2013, University of Oregon

URL: http://hdl.handle.net/1794/12955

► Signaling systems regulate the flow of cellular information by organizing proteins in space and time to coordinate a variety of cellular activities that are critical… (more)

▼ Signaling systems regulate the flow of cellular information by organizing proteins in space and time to coordinate a variety of cellular activities that are critical for the proper development, function, and maintenance of cells. Signaling molecules can exhibit several levels of complexity through the utilization of modular protein interactions, which can generate simple linear behaviors or complex behaviors such as ultrasensitivity. Protein modularity also serves as the basis for the vast protein networks that form the regulatory networks that govern several biological activities. My work focuses on the importance of protein modularity in complex biological systems, in particular the regulatory pathways of spindle positioning. The first part of my work involves the construction of a synthetic regulatory network using modular protein interactions in an effort to understand the complex behavior of the natural spindle orientation regulator Pins. Utilizing well-characterized protein domains and their binding partners, I built an autoinhibited protein switch that can be activated by a small protein domain. We found that the input-output relationship of the synthetic protein switch could be tuned by the simple addition of "decoy" domains, domains that bind and sequester input signal, thereby impeding the onset of the output response to generate an input threshold. By varying the number and affinities of the decoy domains, we found that we could transform a simple linear response into a complex, ultrasensitive one. Thus, modular protein interactions can serve as a source of complex behaviors. The second part of my work focuses on elucidating the molecular mechanisms underlying spindle positioning in the Drosophila neuroblast. I found that Pins orients the mitotic spindle by coordinating two opposite-polarity microtubule motors Dynein and Kinesin-73 through its multiple domains. Kinesin-73 also relies on its modular domain architecture to perform its duties in Pins-mediated spindle positioning, where its N-terminal half functions in coordinating cortical-microtubule capture while its C-terminal half functions as a region necessary for the activation of Dynein. Thus, modular protein design allows for the organization of spindle orientation regulators in space to achieve the complex biological activity that is spindle positioning. This dissertation includes previously published and unpublished coauthored material. Advisors/Committee Members: Bowerman, Bruce (advisor).

Subjects/Keywords: Kinesin; Pins; Spindle orientation; Ultrasensitivity

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APA (6^th Edition):

Lu, M. (2013). The Construction and Deconstruction of Signaling Systems that Regulate Mitotic Spindle Positioning. (Doctoral Dissertation). University of Oregon. Retrieved from http://hdl.handle.net/1794/12955

Chicago Manual of Style (16^th Edition):

Lu, Michelle. “The Construction and Deconstruction of Signaling Systems that Regulate Mitotic Spindle Positioning.” 2013. Doctoral Dissertation, University of Oregon. Accessed January 17, 2021. http://hdl.handle.net/1794/12955.

MLA Handbook (7^th Edition):

Lu, Michelle. “The Construction and Deconstruction of Signaling Systems that Regulate Mitotic Spindle Positioning.” 2013. Web. 17 Jan 2021.

Vancouver:

Lu M. The Construction and Deconstruction of Signaling Systems that Regulate Mitotic Spindle Positioning. [Internet] [Doctoral dissertation]. University of Oregon; 2013. [cited 2021 Jan 17]. Available from: http://hdl.handle.net/1794/12955.

Council of Science Editors:

Lu M. The Construction and Deconstruction of Signaling Systems that Regulate Mitotic Spindle Positioning. [Doctoral Dissertation]. University of Oregon; 2013. Available from: http://hdl.handle.net/1794/12955

Colorado State University

14. Tauchman, Eric Cary. Stable kinetochore-microtubule attachment is sufficient to satisfy the spindle assembly checkpoint.

Degree: PhD, Cell and Molecular Biology, 2016, Colorado State University

URL: http://hdl.handle.net/10217/176748

► During mitosis, duplicated sister chromatids attach to microtubules emanating from opposing sides of the bipolar spindle through large protein complexes called kinetochores. The kinetochore proteins… (more)

▼ During mitosis, duplicated sister chromatids attach to microtubules emanating from opposing sides of the bipolar spindle through large protein complexes called kinetochores. The kinetochore proteins that bind spindle microtubules are exquisitely regulated to ensure correct segregation of genetic material at mitotic exit. Aurora B Kinase (ABK) phosphorylates Hec1, a protein that directly binds microtubules. This is critical for enabling the release of incorrect kinetochore-microtubule attachments. Hec1 has nine ABK phosphorylation sites on its tail domain allowing for precise control over binding affinity. We find that at least 7 of these sites are required for wild-type kinetochore-microtubule (K-MT) attachment stability as evaluated by inter-kinetochore distance measures and chromosome alignment capability. We further observe that several sites may have more influence on K-MT attachment stability than others. Hec1 mutations preventing phosphorylation increase kinetochore-microtubule attachment stability. In the absence of stable kinetochore–microtubule (K-MT) attachments, a cell surveillance mechanism known as the spindle assembly checkpoint (SAC) produces an inhibitory signal that prevents anaphase onset. Precisely how the inhibitory SAC signal is extinguished in response to microtubule attachment remains unresolved. To address this, we induced formation of hyper-stable kinetochore–microtubule attachments in human cells using a non-phosphorylatable Hec1mutant, a core component of the attachment machinery. This mutant reduced the ability of ABK to cause release of erroneous K-MT so we could test the hypothesis that stable K-MT attachments satisfy the SAC even if those attachments deviate from the canonical bipolar form. We find that stable attachments are sufficient to satisfy the SAC in the absence of sister kinetochore bi-orientation and strikingly in the absence of detectable microtubule pulling forces or tension. Furthermore, we find that SAC satisfaction occurs despite the absence of large changes in intra-kinetochore distance, suggesting that substantial kinetochore stretching is not required for quenching the SAC signal. These results indicate a conformational change(s), within the kinetochore that occurs upon stable kinetochore-microtubule binding causes the eviction of SAC proteins. This advance in our understanding of SAC function offers insight into the mode of action and the variation in cellular response to mitotic arrest therapies often used in treatments of cancers. Advisors/Committee Members: DeLuca, Jennifer G. (advisor), Chen, Chaoping (committee member), Ross, Eric (committee member), Wilusz, Carol (committee member).

Subjects/Keywords: microtubule; kinetochore; spindle assembly checkpoint

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APA (6^th Edition):

Tauchman, E. C. (2016). Stable kinetochore-microtubule attachment is sufficient to satisfy the spindle assembly checkpoint. (Doctoral Dissertation). Colorado State University. Retrieved from http://hdl.handle.net/10217/176748

Chicago Manual of Style (16^th Edition):

Tauchman, Eric Cary. “Stable kinetochore-microtubule attachment is sufficient to satisfy the spindle assembly checkpoint.” 2016. Doctoral Dissertation, Colorado State University. Accessed January 17, 2021. http://hdl.handle.net/10217/176748.

MLA Handbook (7^th Edition):

Tauchman, Eric Cary. “Stable kinetochore-microtubule attachment is sufficient to satisfy the spindle assembly checkpoint.” 2016. Web. 17 Jan 2021.

Vancouver:

Tauchman EC. Stable kinetochore-microtubule attachment is sufficient to satisfy the spindle assembly checkpoint. [Internet] [Doctoral dissertation]. Colorado State University; 2016. [cited 2021 Jan 17]. Available from: http://hdl.handle.net/10217/176748.

Council of Science Editors:

Tauchman EC. Stable kinetochore-microtubule attachment is sufficient to satisfy the spindle assembly checkpoint. [Doctoral Dissertation]. Colorado State University; 2016. Available from: http://hdl.handle.net/10217/176748

Brno University of Technology

15. Švarc, Pavel. Konstrukce vřetene soustruhu: Design of lathe spindl.

Degree: 2018, Brno University of Technology

URL: http://hdl.handle.net/11012/7891

► Bachelor thesis is focused on one of the most important parts of lathe – spindle. Thesis is divided into two main parts. First of them… (more)

Subjects/Keywords: Vřeteno; soustruh; konstrukce vřetene; vřetenová ložiska; náhon vřetene; Spindle; lathe; designing of spindle; spindle bearings; spindle drive

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APA (6^th Edition):

Švarc, P. (2018). Konstrukce vřetene soustruhu: Design of lathe spindl. (Thesis). Brno University of Technology. Retrieved from http://hdl.handle.net/11012/7891

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Švarc, Pavel. “Konstrukce vřetene soustruhu: Design of lathe spindl.” 2018. Thesis, Brno University of Technology. Accessed January 17, 2021. http://hdl.handle.net/11012/7891.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Švarc, Pavel. “Konstrukce vřetene soustruhu: Design of lathe spindl.” 2018. Web. 17 Jan 2021.

Vancouver:

Švarc P. Konstrukce vřetene soustruhu: Design of lathe spindl. [Internet] [Thesis]. Brno University of Technology; 2018. [cited 2021 Jan 17]. Available from: http://hdl.handle.net/11012/7891.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Švarc P. Konstrukce vřetene soustruhu: Design of lathe spindl. [Thesis]. Brno University of Technology; 2018. Available from: http://hdl.handle.net/11012/7891

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Edinburgh

16. Głuszek-Kustusz, Agnieszka Agata. Dissecting mechanisms of chromosomemicrotubule interaction in oocytes by new imaging tools.

Degree: PhD, 2014, University of Edinburgh

URL: http://hdl.handle.net/1842/18009

► Chromosome alignment and orientation within the spindle in mitosis and meiosis are determined by chromosome-microtubule interaction. Evidence suggests that within the acentrosomal spindle the mechanism… (more)

Subjects/Keywords: 571.8; kinetochore; spindle; oocytes

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APA (6^th Edition):

Głuszek-Kustusz, A. A. (2014). Dissecting mechanisms of chromosomemicrotubule interaction in oocytes by new imaging tools. (Doctoral Dissertation). University of Edinburgh. Retrieved from http://hdl.handle.net/1842/18009

Chicago Manual of Style (16^th Edition):

Głuszek-Kustusz, Agnieszka Agata. “Dissecting mechanisms of chromosomemicrotubule interaction in oocytes by new imaging tools.” 2014. Doctoral Dissertation, University of Edinburgh. Accessed January 17, 2021. http://hdl.handle.net/1842/18009.

MLA Handbook (7^th Edition):

Głuszek-Kustusz, Agnieszka Agata. “Dissecting mechanisms of chromosomemicrotubule interaction in oocytes by new imaging tools.” 2014. Web. 17 Jan 2021.

Vancouver:

Głuszek-Kustusz AA. Dissecting mechanisms of chromosomemicrotubule interaction in oocytes by new imaging tools. [Internet] [Doctoral dissertation]. University of Edinburgh; 2014. [cited 2021 Jan 17]. Available from: http://hdl.handle.net/1842/18009.

Council of Science Editors:

Głuszek-Kustusz AA. Dissecting mechanisms of chromosomemicrotubule interaction in oocytes by new imaging tools. [Doctoral Dissertation]. University of Edinburgh; 2014. Available from: http://hdl.handle.net/1842/18009

University of New South Wales

17. Tilia, Liza. Is There a Link Between Oocyte Meiotic Spindle Normality and Embryo Euploidy?.

Degree: Women's & Children's Health, 2019, University of New South Wales

URL: http://handle.unsw.edu.au/1959.4/70058

► Aim: To explore the relationship between oocyte meiotic spindle (OMS) morphology, visualised by polarised light microscopy (PLM) at the time of intracytoplasmic sperm injection (ICSI),… (more)

▼ Aim: To explore the relationship between oocyte meiotic spindle (OMS) morphology, visualised by polarised light microscopy (PLM) at the time of intracytoplasmic sperm injection (ICSI), with the genetic make-up of the resulting embryo as determined by preimplantation genetic testing (PGT).Method: Three separate studies were performed using similar methodologies. For all studies, the OMS was visualised by PLM at the time of ICSI and classified as normal or abnormal (dysmorphic, translucent, telophase and not visible). The resulting embryos were cultured and PGT was performed by whole genome analysis.Study 1: A single blastomere was biopsied from suitably developed cleavage stage embryos. PGT was performed by whole genome analysis (WGA).Study 2: Trophectoderm cells (3-5) were biopsied from suitably developed blastocysts and PGT performed by WGA.Study 3: Oocytes at metaphase II and late-maturing oocytes (metaphase I at denudation) were compared for developmental markers such as fertilisation, embryo quality and OMS morphology.The data from these studies was analysed by generalised estimating equation to assess the association of OMS morphologies with developmental markers and the resulting embryo ploidy.Result: The probability of embryo euploidy was associated with a normal compared to abnormal OMS whether embryo ploidy was determined at cleavage or blastocyst stage. The sub-classifications of OMS morphology resulted in similar associations with embryo euploidy at both cleavage and blastocyst stage: There was no significant difference in euploidy rate between normal and dysmorphic OMSs. Translucent and no visible OMSs were associated with embryo aneuploidy. No euploid embryos resulted from oocytes with a telophase OMS. The frequency of all OMS morphology types in late-maturing oocytes was significantly different to their sibling mature oocytes.Conclusion: A link does exist between OMS normality and embryo euploidy. Normal OMS morphology is associated with cleavage and blastocyst stage embryo euploidy compared to oocytes with abnormal OMS morphology. Translucent and not visible OMS morphologies are significantly more likely to be associated with aneuploidy, while oocytes identified in telophase were never associated with a euploid embryo, regardless of the developmental stage of PGT. This is the first study to find an association between OMS morphology and embryo ploidy anda non-invasive method of predicting the resulting embryo ploidy. Advisors/Committee Members: Chapman, Michael, Women's & Children's Health, Faculty of Medicine, UNSW, Venetis, Christos, Women's & Children's Health, Faculty of Medicine, UNSW, Cooke, Simon, Women's & Children's Health, Faculty of Medicine, UNSW.

Subjects/Keywords: Aneuploidy; Oocyte; Meiotic spindle

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APA (6^th Edition):

Tilia, L. (2019). Is There a Link Between Oocyte Meiotic Spindle Normality and Embryo Euploidy?. (Doctoral Dissertation). University of New South Wales. Retrieved from http://handle.unsw.edu.au/1959.4/70058

Chicago Manual of Style (16^th Edition):

Tilia, Liza. “Is There a Link Between Oocyte Meiotic Spindle Normality and Embryo Euploidy?.” 2019. Doctoral Dissertation, University of New South Wales. Accessed January 17, 2021. http://handle.unsw.edu.au/1959.4/70058.

MLA Handbook (7^th Edition):

Tilia, Liza. “Is There a Link Between Oocyte Meiotic Spindle Normality and Embryo Euploidy?.” 2019. Web. 17 Jan 2021.

Vancouver:

Tilia L. Is There a Link Between Oocyte Meiotic Spindle Normality and Embryo Euploidy?. [Internet] [Doctoral dissertation]. University of New South Wales; 2019. [cited 2021 Jan 17]. Available from: http://handle.unsw.edu.au/1959.4/70058.

Council of Science Editors:

Tilia L. Is There a Link Between Oocyte Meiotic Spindle Normality and Embryo Euploidy?. [Doctoral Dissertation]. University of New South Wales; 2019. Available from: http://handle.unsw.edu.au/1959.4/70058

University of Texas – Austin

18. Shaikh, Tamanna Abdulhakim. Muscle spindle responses following fatigue and ischemia.

Degree: MSin Kinesiology, Kinesiology, 2011, University of Texas – Austin

URL: http://hdl.handle.net/2152/ETD-UT-2011-08-4229

► The purpose of this study was to determine whether ischemia would enhance muscle spindle responses to tendon tap and vibration during submaximal fatiguing contractions in… (more)

Subjects/Keywords: Muscle spindle; Motor units; Ischemia

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APA (6^th Edition):

Shaikh, T. A. (2011). Muscle spindle responses following fatigue and ischemia. (Masters Thesis). University of Texas – Austin. Retrieved from http://hdl.handle.net/2152/ETD-UT-2011-08-4229

Chicago Manual of Style (16^th Edition):

Shaikh, Tamanna Abdulhakim. “Muscle spindle responses following fatigue and ischemia.” 2011. Masters Thesis, University of Texas – Austin. Accessed January 17, 2021. http://hdl.handle.net/2152/ETD-UT-2011-08-4229.

MLA Handbook (7^th Edition):

Shaikh, Tamanna Abdulhakim. “Muscle spindle responses following fatigue and ischemia.” 2011. Web. 17 Jan 2021.

Vancouver:

Shaikh TA. Muscle spindle responses following fatigue and ischemia. [Internet] [Masters thesis]. University of Texas – Austin; 2011. [cited 2021 Jan 17]. Available from: http://hdl.handle.net/2152/ETD-UT-2011-08-4229.

Council of Science Editors:

Shaikh TA. Muscle spindle responses following fatigue and ischemia. [Masters Thesis]. University of Texas – Austin; 2011. Available from: http://hdl.handle.net/2152/ETD-UT-2011-08-4229

University of California – San Francisco

19. Hueschen, Christina Lynn. Building the Mitotic Spindle: Spatial Regulation and Function of Force at Microtubule Minus-Ends.

Degree: Biomedical Sciences, 2018, University of California – San Francisco

URL: http://www.escholarship.org/uc/item/6389717b

► Each time a cell divides, the microtubule cytoskeleton self-organizes into the metaphase spindle: an ellipsoidal steady-state structure that holds its stereotyped shape despite microtubule turnover… (more)

Subjects/Keywords: Cellular biology; Biophysics; dynein; microtubules; mitosis; self-organization; spindle; spindle mechanics

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APA (6^th Edition):

Hueschen, C. L. (2018). Building the Mitotic Spindle: Spatial Regulation and Function of Force at Microtubule Minus-Ends. (Thesis). University of California – San Francisco. Retrieved from http://www.escholarship.org/uc/item/6389717b

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Hueschen, Christina Lynn. “Building the Mitotic Spindle: Spatial Regulation and Function of Force at Microtubule Minus-Ends.” 2018. Thesis, University of California – San Francisco. Accessed January 17, 2021. http://www.escholarship.org/uc/item/6389717b.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Hueschen, Christina Lynn. “Building the Mitotic Spindle: Spatial Regulation and Function of Force at Microtubule Minus-Ends.” 2018. Web. 17 Jan 2021.

Vancouver:

Hueschen CL. Building the Mitotic Spindle: Spatial Regulation and Function of Force at Microtubule Minus-Ends. [Internet] [Thesis]. University of California – San Francisco; 2018. [cited 2021 Jan 17]. Available from: http://www.escholarship.org/uc/item/6389717b.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Hueschen CL. Building the Mitotic Spindle: Spatial Regulation and Function of Force at Microtubule Minus-Ends. [Thesis]. University of California – San Francisco; 2018. Available from: http://www.escholarship.org/uc/item/6389717b

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Iowa State University

20. Yao, Changfu. A nuclear-derived proteinaceous matrix embeds the microtubule spindle apparatus during mitosis.

Degree: 2013, Iowa State University

URL: https://lib.dr.iastate.edu/etd/13478

► ABSTRACT< The nucleus undergoes a dramatic reorganization as the cell prepares to segregate its duplicated chromosomes during cell division. For many years s, the prevailing… (more)

▼ ABSTRACT< The nucleus undergoes a dramatic reorganization as the cell prepares to segregate its duplicated chromosomes during cell division. For many years s, the prevailing view was that in open mitosis the nucleus completely disassembled during early mitotic stages, thus enabling cytoplasmic microtubules emanating from the separated centrosomes to form a mitotic spindle. This cytocentric view largely discounted any nuclear contributions to regulation of mitotic progression (Johansen and Johansen, 2009; De Souza and Osmani, 2009; Simon and Wilson, 2011; Sandquist et al., 2011). A spindle matrix has long been proposed to serve as a relatively stable or elastic molecular matrix that interacts with the microtubule spindle apparatus, based on the consideration of a mechanical and functional support for the stabilization of the microtubule spindle during force generation; however, whether such a structure exists and its molecular and structural composition has remained controversial. In Drosophila we have identified four nuclear proteins, Skeletor, Chromator, Megator, and EAST from two different nuclear compartments that interact with each other (reviewed in Johansen et al., 2012) and that redistribute during prophase to form a dynamic, gel-like spindle matrix that embeds the microtubule spindle apparatus, stretching from pole-to-pole (Yao et al., 2012a). In this dissertation, I present the dynamic distribution of the spindle matrix components using a live imaging approach by expressing GFP tagged spindle matrix complex components in Drosophila syncytial embryos. As shown in the dissertation, this matrix forms prior to nuclear envelope breakdown and specific interactions between spindle matrix molecules are necessary for complex formation and cohesion (Yao et al., 2012a). When microtubules are depolymerized with colchicine just prior to metaphase, the spindle matrix contracts and coalesces around the chromosomes suggesting that microtubules act as "struts" stretching the spindle matrix. Furthermore, in colchicine treated embryos free tubulin accumulates co-extensively with the spindle matrix proteins suggesting that this enrichment is dependent on one or more proteins within the spindle matrix with tubulin binding activity. Biochemical interaction assays show a potential direct interaction between Chromator and polymerized microtubules or free tubulin. This tubulin binding activity of Chromator provides support for the hypothesis that reorganization of nuclear proteins into a spindle matrix may play a wider functional role in spatially regulating cell cycle progression factors in conjunction with contributing to microtubule spindle assembly and dynamics. Moreover, we have demonstrated that the coiled-coil domain of Megator is responsible and required for Megator's spindle matrix localization and function. During interphase Chromator localizes to the interband region of Drosophila polytene chromosomes and is required for the maintenance of chromosome morphology. Here I show that the N-terminus domain of Chromator is…

Subjects/Keywords: Chromator; Megator; Microtubules; Mitotic spindle; Spindle matrix; Biochemistry; Biology; Cell Biology

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APA (6^th Edition):

Yao, C. (2013). A nuclear-derived proteinaceous matrix embeds the microtubule spindle apparatus during mitosis. (Thesis). Iowa State University. Retrieved from https://lib.dr.iastate.edu/etd/13478

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Yao, Changfu. “A nuclear-derived proteinaceous matrix embeds the microtubule spindle apparatus during mitosis.” 2013. Thesis, Iowa State University. Accessed January 17, 2021. https://lib.dr.iastate.edu/etd/13478.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Yao, Changfu. “A nuclear-derived proteinaceous matrix embeds the microtubule spindle apparatus during mitosis.” 2013. Web. 17 Jan 2021.

Vancouver:

Yao C. A nuclear-derived proteinaceous matrix embeds the microtubule spindle apparatus during mitosis. [Internet] [Thesis]. Iowa State University; 2013. [cited 2021 Jan 17]. Available from: https://lib.dr.iastate.edu/etd/13478.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Yao C. A nuclear-derived proteinaceous matrix embeds the microtubule spindle apparatus during mitosis. [Thesis]. Iowa State University; 2013. Available from: https://lib.dr.iastate.edu/etd/13478

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Brno University of Technology

21. Zábojník, Petr. Soustružnické vřeteno: Lathe spindle.

Degree: 2019, Brno University of Technology

URL: http://hdl.handle.net/11012/3557

► Bachelor’s thesis will be talking about one of main parts of lathe, about spindle. In first part I’m going to make a research in this… (more)

Subjects/Keywords: Vřeteno; konstrukce vřetene; soustruh; Spindle; designing of spindle; lathe

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APA (6^th Edition):

Zábojník, P. (2019). Soustružnické vřeteno: Lathe spindle. (Thesis). Brno University of Technology. Retrieved from http://hdl.handle.net/11012/3557

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Zábojník, Petr. “Soustružnické vřeteno: Lathe spindle.” 2019. Thesis, Brno University of Technology. Accessed January 17, 2021. http://hdl.handle.net/11012/3557.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Zábojník, Petr. “Soustružnické vřeteno: Lathe spindle.” 2019. Web. 17 Jan 2021.

Vancouver:

Zábojník P. Soustružnické vřeteno: Lathe spindle. [Internet] [Thesis]. Brno University of Technology; 2019. [cited 2021 Jan 17]. Available from: http://hdl.handle.net/11012/3557.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Zábojník P. Soustružnické vřeteno: Lathe spindle. [Thesis]. Brno University of Technology; 2019. Available from: http://hdl.handle.net/11012/3557

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Brno University of Technology

22. Sobotka, Radek. Konstrukce vřetene vrtacího stroje: Design of boring machine spindle.

Degree: 2019, Brno University of Technology

URL: http://hdl.handle.net/11012/31732

► The subject of this bachelor’s thesis is the design of a drilling machine. The first part consists of a research in the field of drilling… (more)

Subjects/Keywords: Vřeteno; vrtací stroj; vřetenová ložiska; pohon vřetena; parametry vřetena; konstrukce vřetena; Spindle; drilling machine; spindle bearings; spindle drive; spindle characteristics; spindle design

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APA (6^th Edition):

Sobotka, R. (2019). Konstrukce vřetene vrtacího stroje: Design of boring machine spindle. (Thesis). Brno University of Technology. Retrieved from http://hdl.handle.net/11012/31732

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Sobotka, Radek. “Konstrukce vřetene vrtacího stroje: Design of boring machine spindle.” 2019. Thesis, Brno University of Technology. Accessed January 17, 2021. http://hdl.handle.net/11012/31732.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Sobotka, Radek. “Konstrukce vřetene vrtacího stroje: Design of boring machine spindle.” 2019. Web. 17 Jan 2021.

Vancouver:

Sobotka R. Konstrukce vřetene vrtacího stroje: Design of boring machine spindle. [Internet] [Thesis]. Brno University of Technology; 2019. [cited 2021 Jan 17]. Available from: http://hdl.handle.net/11012/31732.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Sobotka R. Konstrukce vřetene vrtacího stroje: Design of boring machine spindle. [Thesis]. Brno University of Technology; 2019. Available from: http://hdl.handle.net/11012/31732

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Brno University of Technology

23. Slavíček, Josef. Konstrukce vřetene brusky: Design of grinding spindl.

Degree: 2019, Brno University of Technology

URL: http://hdl.handle.net/11012/3449

► The subject of this bachelor thesis is the construction of grinding machine spindle. The work is divided into two main parts. The first part is… (more)

Subjects/Keywords: Vřeteno; konstrukce vřetene; tuhost vřetene; brousící stroj; hydrostatické vřeteno; elektrovřeteno; náhon vřetene.; Spindle; construction of spindle; rigidity of spindle; grinding machine; hydrostatic spindle; electrospindle; drive of spindle.

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APA (6^th Edition):

Slavíček, J. (2019). Konstrukce vřetene brusky: Design of grinding spindl. (Thesis). Brno University of Technology. Retrieved from http://hdl.handle.net/11012/3449

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Slavíček, Josef. “Konstrukce vřetene brusky: Design of grinding spindl.” 2019. Thesis, Brno University of Technology. Accessed January 17, 2021. http://hdl.handle.net/11012/3449.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Slavíček, Josef. “Konstrukce vřetene brusky: Design of grinding spindl.” 2019. Web. 17 Jan 2021.

Vancouver:

Slavíček J. Konstrukce vřetene brusky: Design of grinding spindl. [Internet] [Thesis]. Brno University of Technology; 2019. [cited 2021 Jan 17]. Available from: http://hdl.handle.net/11012/3449.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Slavíček J. Konstrukce vřetene brusky: Design of grinding spindl. [Thesis]. Brno University of Technology; 2019. Available from: http://hdl.handle.net/11012/3449

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Brno University of Technology

24. Šatný, Patrik. Návrh vřeteníku malého soustruhu: Design of spindle for a small lathe.

Degree: 2020, Brno University of Technology

URL: http://hdl.handle.net/11012/192957

► The thesis deals with the design of a headstock for a small lathe. The first part of the thesis deals with the description of individual… (more)

Subjects/Keywords: Vřeteník; vřeteno; elektrovřeteno; vřetenová ložiska; vřetenová hřídel; soustružnické centrum; Headstock; spindle; electrospindle; spindle bearings; spindle shaft; turning center

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APA (6^th Edition):

Šatný, P. (2020). Návrh vřeteníku malého soustruhu: Design of spindle for a small lathe. (Thesis). Brno University of Technology. Retrieved from http://hdl.handle.net/11012/192957

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Šatný, Patrik. “Návrh vřeteníku malého soustruhu: Design of spindle for a small lathe.” 2020. Thesis, Brno University of Technology. Accessed January 17, 2021. http://hdl.handle.net/11012/192957.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Šatný, Patrik. “Návrh vřeteníku malého soustruhu: Design of spindle for a small lathe.” 2020. Web. 17 Jan 2021.

Vancouver:

Šatný P. Návrh vřeteníku malého soustruhu: Design of spindle for a small lathe. [Internet] [Thesis]. Brno University of Technology; 2020. [cited 2021 Jan 17]. Available from: http://hdl.handle.net/11012/192957.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Šatný P. Návrh vřeteníku malého soustruhu: Design of spindle for a small lathe. [Thesis]. Brno University of Technology; 2020. Available from: http://hdl.handle.net/11012/192957

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Brno University of Technology

25. Žádník, Lubomír. Návrh vřeteníku soustružnického centra: Design of cartridge spindle for turning machines.

Degree: 2020, Brno University of Technology

URL: http://hdl.handle.net/11012/192960

► The diploma thesis deals with the design of a built-in headstock of a turning center. The aim of the work is the design of a… (more)

Subjects/Keywords: Vřeteno; vřeteník; soustružnické centrum; uložení vřetene; pohon vřetene; Spindle; headstock; turning centre; spindle Bering; spindle drive

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APA (6^th Edition):

Žádník, L. (2020). Návrh vřeteníku soustružnického centra: Design of cartridge spindle for turning machines. (Thesis). Brno University of Technology. Retrieved from http://hdl.handle.net/11012/192960

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Žádník, Lubomír. “Návrh vřeteníku soustružnického centra: Design of cartridge spindle for turning machines.” 2020. Thesis, Brno University of Technology. Accessed January 17, 2021. http://hdl.handle.net/11012/192960.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Žádník, Lubomír. “Návrh vřeteníku soustružnického centra: Design of cartridge spindle for turning machines.” 2020. Web. 17 Jan 2021.

Vancouver:

Žádník L. Návrh vřeteníku soustružnického centra: Design of cartridge spindle for turning machines. [Internet] [Thesis]. Brno University of Technology; 2020. [cited 2021 Jan 17]. Available from: http://hdl.handle.net/11012/192960.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Žádník L. Návrh vřeteníku soustružnického centra: Design of cartridge spindle for turning machines. [Thesis]. Brno University of Technology; 2020. Available from: http://hdl.handle.net/11012/192960

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Brno University of Technology

26. Burian, Viktor. Návrh vřeteníku frézovacího centra: Design of cartridge spindle for milling machines.

Degree: 2019, Brno University of Technology

URL: http://hdl.handle.net/11012/83130

► This master´s thesis deals with the design of a cartridge spindle for milling machines. The aim of the work is to design a spindle with… (more)

Subjects/Keywords: vřeteník; vřeteno; frézovací centrum; vřetenová ložiska; vřetenová hřídel; headstock; spindle; milling machines; spindle bearings; spindle shaft

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APA (6^th Edition):

Burian, V. (2019). Návrh vřeteníku frézovacího centra: Design of cartridge spindle for milling machines. (Thesis). Brno University of Technology. Retrieved from http://hdl.handle.net/11012/83130

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Burian, Viktor. “Návrh vřeteníku frézovacího centra: Design of cartridge spindle for milling machines.” 2019. Thesis, Brno University of Technology. Accessed January 17, 2021. http://hdl.handle.net/11012/83130.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Burian, Viktor. “Návrh vřeteníku frézovacího centra: Design of cartridge spindle for milling machines.” 2019. Web. 17 Jan 2021.

Vancouver:

Burian V. Návrh vřeteníku frézovacího centra: Design of cartridge spindle for milling machines. [Internet] [Thesis]. Brno University of Technology; 2019. [cited 2021 Jan 17]. Available from: http://hdl.handle.net/11012/83130.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Burian V. Návrh vřeteníku frézovacího centra: Design of cartridge spindle for milling machines. [Thesis]. Brno University of Technology; 2019. Available from: http://hdl.handle.net/11012/83130

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Rochester Institute of Technology

27. Hoyt, Jamie. Machine tool spindle design.

Degree: Mechanical Engineering, 2000, Rochester Institute of Technology

URL: https://scholarworks.rit.edu/theses/7199

► In modern machine tool applications the performance of a machine tool is judged by its ability to produce work-pieces accurately and efficiently. The stiffness of… (more)

Subjects/Keywords: Mechanical engineering; Spindle

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APA (6^th Edition):

Hoyt, J. (2000). Machine tool spindle design. (Thesis). Rochester Institute of Technology. Retrieved from https://scholarworks.rit.edu/theses/7199

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Hoyt, Jamie. “Machine tool spindle design.” 2000. Thesis, Rochester Institute of Technology. Accessed January 17, 2021. https://scholarworks.rit.edu/theses/7199.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Hoyt, Jamie. “Machine tool spindle design.” 2000. Web. 17 Jan 2021.

Vancouver:

Hoyt J. Machine tool spindle design. [Internet] [Thesis]. Rochester Institute of Technology; 2000. [cited 2021 Jan 17]. Available from: https://scholarworks.rit.edu/theses/7199.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Hoyt J. Machine tool spindle design. [Thesis]. Rochester Institute of Technology; 2000. Available from: https://scholarworks.rit.edu/theses/7199

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Utah

28. Ross, Thomas James. Microelectric discharge machining spindle design.

Degree: MS, Mechanical Engineering, 2014, University of Utah

URL: http://content.lib.utah.edu/cdm/singleitem/collection/etd3/id/3381/rec/1579

► Technological advancements have created a demand for ever more complex components with extremely small features, high aspect ratios, and tight tolerances. Components are also being… (more)

▼ Technological advancements have created a demand for ever more complex components with extremely small features, high aspect ratios, and tight tolerances. Components are also being made from harder materials, which are more difficult to produce with traditional machining methods. Electric discharge machining (EDM) offers a manufacturing method that addresses these issues. Micro electric discharge milling is a powerful method that rotates the electrode and enables the cutting of complex 3-D pockets with standard electrodes.In order to meet some of the challenges in the micro manufacturing industry, an micro electric discharge milling spindle has been designed. This design uses a standard industrial collet, is capable of loading a large variety of electrode sizes and shapes, and incorporates optimal dielectric flushing. The spindle design offers injection flushing through the electrode and side flushing for the use of solid electrodes. This spindle design also offers the unique feature of variable flushing pressure. The spindle varies the injection pressure automatically to maintain a constant flow rate of dielectric fluid through the electrode as the electrode becomes shorter. The spindle is also capable of automatically feeding and fixturing the electrode as it wears down. The addition of a C-axis gives the spindle the unique ability to rotationally orient the tool. When the C-axis ability of the spindle is used in conjunction with wire electric discharge grinding, the machine is given the ability to produce a large variety of electrode shapes with extremely high aspect ratios and small features.This spindle design offers an economical versatile and compact solution to micro-electric discharge milling and can be easily placed into a CNC machine platform for accurate creation of complex features. The electrode fixturing range, variable dielectric pressure, and C-axis capabilities of this design are unique to this design and are not offered on the current market.

Subjects/Keywords: Design; Discharge; Machining; Microelectric; Mill; Spindle

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APA (6^th Edition):

Ross, T. J. (2014). Microelectric discharge machining spindle design. (Masters Thesis). University of Utah. Retrieved from http://content.lib.utah.edu/cdm/singleitem/collection/etd3/id/3381/rec/1579

Chicago Manual of Style (16^th Edition):

Ross, Thomas James. “Microelectric discharge machining spindle design.” 2014. Masters Thesis, University of Utah. Accessed January 17, 2021. http://content.lib.utah.edu/cdm/singleitem/collection/etd3/id/3381/rec/1579.

MLA Handbook (7^th Edition):

Ross, Thomas James. “Microelectric discharge machining spindle design.” 2014. Web. 17 Jan 2021.

Vancouver:

Ross TJ. Microelectric discharge machining spindle design. [Internet] [Masters thesis]. University of Utah; 2014. [cited 2021 Jan 17]. Available from: http://content.lib.utah.edu/cdm/singleitem/collection/etd3/id/3381/rec/1579.

Council of Science Editors:

Ross TJ. Microelectric discharge machining spindle design. [Masters Thesis]. University of Utah; 2014. Available from: http://content.lib.utah.edu/cdm/singleitem/collection/etd3/id/3381/rec/1579

Universiteit Utrecht

29. Tas, R.P. The role of the centrosome in mitotic spindle formation.

Degree: 2014, Universiteit Utrecht

URL: http://dspace.library.uu.nl:8080/handle/1874/296987

► During cell division, correct chromosome segregation between the two daughter cells is important to maintain the genetic balance in the organism. A bipolar mitotic spindle… (more)

Subjects/Keywords: Centrosome; non-centrosomal; microtubule; spindle formation; review

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APA (6^th Edition):

Tas, R. P. (2014). The role of the centrosome in mitotic spindle formation. (Masters Thesis). Universiteit Utrecht. Retrieved from http://dspace.library.uu.nl:8080/handle/1874/296987

Chicago Manual of Style (16^th Edition):

Tas, R P. “The role of the centrosome in mitotic spindle formation.” 2014. Masters Thesis, Universiteit Utrecht. Accessed January 17, 2021. http://dspace.library.uu.nl:8080/handle/1874/296987.

MLA Handbook (7^th Edition):

Tas, R P. “The role of the centrosome in mitotic spindle formation.” 2014. Web. 17 Jan 2021.

Vancouver:

Tas RP. The role of the centrosome in mitotic spindle formation. [Internet] [Masters thesis]. Universiteit Utrecht; 2014. [cited 2021 Jan 17]. Available from: http://dspace.library.uu.nl:8080/handle/1874/296987.

Council of Science Editors:

Tas RP. The role of the centrosome in mitotic spindle formation. [Masters Thesis]. Universiteit Utrecht; 2014. Available from: http://dspace.library.uu.nl:8080/handle/1874/296987

University of Manchester

30. Hewitt, Laura. Using a novel small molecule inhibitor to investigate the role of Mps1 kinase activity.

Degree: 2011, University of Manchester

URL: http://www.manchester.ac.uk/escholar/uk-ac-man-scw:104768

► During mitosis, accurate chromosome segregation is essential: gain or loss of genetic information can be detrimental to cell viability, or promote tumourigenesis. The mitotic checkpoint… (more)

▼ During mitosis, accurate chromosome segregation is essential: gain or loss of genetic information can be detrimental to cell viability, or promote tumourigenesis. The mitotic checkpoint (also known as the spindle assembly checkpoint or SAC) ensures accurate chromosome segregation by delaying cell cycle progression until accuracy can be guaranteed. Mps1 is a protein kinase that is crucial for mitotic checkpoint signalling and also for proper chromosome alignment at metaphase. However, the precise role of Mps1’s catalytic activity is still unclear. Here, I present AZ3146, a novel small molecule inhibitor of Mps1. AZ3146 inhibits recombinant Mps1 in vitro with an IC50 of ~35 nM, and has low activity against a panel of 50 kinases, suggesting a reasonable degree of selectivity. As predicted for an Mps1 inhibitor, AZ1346 treatment led to spindle checkpoint malfunction in cells, accelerated mitotic timing, and perturbed the kinetochore localisation of the checkpoint effector Mad2. AZ3146 has a negative effect on cell viability, suggesting it leads to detrimental missegregations. Thus, the cellular effects of AZ3146 are consistent with Mps1 inhibition, and I was able to use the compound confidently as a tool to further probe the role of Mps1 activity in cells.Strikingly, levels of Mps1 increased at unattached kinetochores following inhibition of its kinase activity, suggesting Mps1’s kinetochore localisation is regulated by its own activity. A kinase-dead GFP-Mps1 fusion protein only accumulated at kinetochores in the absence of endogenous, active Mps1, implicating intra-molecular interactions in regulation of Mps1’s kinetochore localisation. I confirm a role for Mps1 in the mechanism of chromosome alignment, but in contrast to previous reports I did not detect a decrease in Aurora B activity following Mps1 inhibition. On the contrary, both Mps1’s phosphorylation status and its kinetochore localisation were affected by treatment with the Aurora B inhibitor ZM447439, placing Mps1 downstream of Aurora B. As an alternative explanation for the alignment defect in cells with reduced Mps1 activity, I found that levels of the plus-end directed kinesin Cenp-E were markedly decreased at unaligned kinetochores. I propose a model in which catalytically active Mps1’s auto-release from kinetochores simultaneously promotes both mitotic checkpoint signalling and chromosome alignment by facilitating Mad2 dimerisation and Cenp-E binding at unattached kinetochores. Advisors/Committee Members: Taylor, Stephen.

Subjects/Keywords: Mps1; mitosis; mitotic checkpoint; spindle assembly checkpoint

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APA (6^th Edition):

Hewitt, L. (2011). Using a novel small molecule inhibitor to investigate the role of Mps1 kinase activity. (Doctoral Dissertation). University of Manchester. Retrieved from http://www.manchester.ac.uk/escholar/uk-ac-man-scw:104768

Chicago Manual of Style (16^th Edition):

Hewitt, Laura. “Using a novel small molecule inhibitor to investigate the role of Mps1 kinase activity.” 2011. Doctoral Dissertation, University of Manchester. Accessed January 17, 2021. http://www.manchester.ac.uk/escholar/uk-ac-man-scw:104768.

MLA Handbook (7^th Edition):

Hewitt, Laura. “Using a novel small molecule inhibitor to investigate the role of Mps1 kinase activity.” 2011. Web. 17 Jan 2021.

Vancouver:

Hewitt L. Using a novel small molecule inhibitor to investigate the role of Mps1 kinase activity. [Internet] [Doctoral dissertation]. University of Manchester; 2011. [cited 2021 Jan 17]. Available from: http://www.manchester.ac.uk/escholar/uk-ac-man-scw:104768.

Council of Science Editors:

Hewitt L. Using a novel small molecule inhibitor to investigate the role of Mps1 kinase activity. [Doctoral Dissertation]. University of Manchester; 2011. Available from: http://www.manchester.ac.uk/escholar/uk-ac-man-scw:104768

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