subject:(spermatogenesis)

California State Polytechnic University – Pomona

1. Prajapati, Gaurav. Premature Sperm Activation Prevented by spe-6 in the Nematode, Caenorhabditis elegans.

Degree: MS, Department of Biological Sciences, 2020, California State Polytechnic University – Pomona

URL: http://hdl.handle.net/10211.3/214949

► Spermatogenesis pauses only at the penultimate stage, the spermatid. The pause ends when an external signal activates the spermatid into a spermatozoon. Activation involves two… (more)

▼ Spermatogenesis pauses only at the penultimate stage, the spermatid. The pause ends when an external signal activates the spermatid into a spermatozoon. Activation involves two pathways: (i) the try-5 pathway, named because the trypsin-like protein TRY-5, in the seminal fluid activates spermatids during mating; and (ii) the spe-8 pathway, named because the SPE-8 group complex transduces the activation signal within the sperm after the cells receive an unknown activation signal. Mutations in the spe-8 group genes (spe-8, -12, -19, -27, -29, and -43) result in spermatids that cannot activate in unmated hermaphrodites. We focus on one target of the SPE-8 complex, SPE-6 a serine-threonine kinase protein. Here, we test the hypothesis that active SPE-6 maintains the spermatid stage, and loss of SPE-6 function results in activation. A suppressor screen from Samuel Ward???s laboratory with spe-27(it132ts) generated numerous suppressor mutations that restore partial fertility. The only gene to be hit multiple times in the screen was spe-6. Previously known null spe-6 mutations stall spermatogenesis at the transition from primary to secondary spermatocytes. In contrast, most spe-6 suppressor mutations cause premature spermatid activation. Five suspected alleles of spe-6 were examined through complementation crosses and sequencing; three of five were confirmed to be spe-6 alleles. The alleles uncovered: zq10, zq11, and zq18 are compared to known spe-6 alleles: hc166, hc187, hc188, and hc190 to get a broader view of spe-6. All alleles except zq11 were able to suppress the sterility of spe-29(it127). Additionally, mutant males had active sperm in their seminal vesicles, while wild type males never harbor active sperm. These suppressor mutations were located in various locations with respect to the 3D structure of the protein, and two others were intronic and altered a splice junction, likely reducing transcript availability. These results suggest that a reduction in SPE-6 can be brought about through loss of function point mutations or loss of transcripts, with the effect that sperm activate prematurely, bypassing the need for the spe-8 group pathway signaling. The unique allele zq11 is an allele specific suppressor of spe-27(it132ts): it does not suppress spe-27(it110) or spe-29(it127). Allele specific suppression vi provides genetic evidence that SPE-6 and SPE-27 proteins interact physically and suggests that SPE-6 is a target of SPE-8 group signaling. The two splicing mutations were replicated in spe-44 using CRISPR/Cas9 in an effort to induce conditional mutation in this important gene. However, neither mutation in spe-44 produced a phenotype. Advisors/Committee Members: LaMunyon, Craig (advisor), Buckley, Nancy (committee member).

Subjects/Keywords: spermatogenesis

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Prajapati, G. (2020). Premature Sperm Activation Prevented by spe-6 in the Nematode, Caenorhabditis elegans. (Masters Thesis). California State Polytechnic University – Pomona. Retrieved from http://hdl.handle.net/10211.3/214949

Chicago Manual of Style (16^th Edition):

Prajapati, Gaurav. “Premature Sperm Activation Prevented by spe-6 in the Nematode, Caenorhabditis elegans.” 2020. Masters Thesis, California State Polytechnic University – Pomona. Accessed March 07, 2021. http://hdl.handle.net/10211.3/214949.

MLA Handbook (7^th Edition):

Prajapati, Gaurav. “Premature Sperm Activation Prevented by spe-6 in the Nematode, Caenorhabditis elegans.” 2020. Web. 07 Mar 2021.

Vancouver:

Prajapati G. Premature Sperm Activation Prevented by spe-6 in the Nematode, Caenorhabditis elegans. [Internet] [Masters thesis]. California State Polytechnic University – Pomona; 2020. [cited 2021 Mar 07]. Available from: http://hdl.handle.net/10211.3/214949.

Council of Science Editors:

Prajapati G. Premature Sperm Activation Prevented by spe-6 in the Nematode, Caenorhabditis elegans. [Masters Thesis]. California State Polytechnic University – Pomona; 2020. Available from: http://hdl.handle.net/10211.3/214949

Oregon State University

2. Longo, Frank J., 1939-. The fine structure of spermatid differentiation in the mussel, Mytilus edulis, Linn.

Degree: PhD, Zoology, 1967, Oregon State University

URL: http://hdl.handle.net/1957/47217

► The spermiogenesis of Mytilus edulis (a marine mussel) has been studied, employing techniques of light and electron microscopy and cytochemical preparations. The observations trace the… (more)

▼ The spermiogenesis of Mytilus edulis (a marine mussel) has been studied, employing techniques of light and electron microscopy and cytochemical preparations. The observations trace the development of the acrosomal complex, the nucleus, the mitochondrial middle-piece, and the flagellum. In early spermatids numerous membrane-bounded proacrosomal vesicles arise from several Golgi complexes and coalesce to form a single large acrosomal vesicle. This comes to rest on the anterior surface of the nucleus and invaginates to become an elongated cone-shaped structure. An electron dense, spherical mass of membranoid sleeve material, also arising from the Golgi complex, adjoins the acrosomal vesicle and later occupies its indentation. In mature spermatozoa the membranoid sleeve lines the cavity of the cone-shaped acrosomal vesicle and surrounds the axial rod. Precursor material of the axial rod is first observed between the nucleus and the acrosomal vesicle as an aggregation of fibers. This later extends posteriorly into the nucleus and anteriorly into the cavity of the acrosomal vesicle. In the mature spermatozoon the fibrous axial rod ends posteriorly in the centriolar region of the middle-piece. The endoplasmic reticulum of early spermatids is a rudimentary system of membrane-bounded canaliculi and flattened vesicles which is predominantly free of ribosomes. Independent or clustered ribosome-like particles are present in the ground cytoplasm. By late spermiogenesis the reticulum has largely disappeared and the ground cytoplasm containing a dense particulate aggregate is sloughed off. In the early spermatid a dense filamentous network of chromatin in the interior and along the periphery of the nucleus increases in size by accretion of the finer karyoplasm. Eventually the interaggregate spaces are obliterated as the large, coarsely granular masses coalesce to form the compact, barrel-shaped nucleus of the sperm. By mid-spermiogenesis the randomly distributed elipsoid mitochondria become localized around the nucleus. The association with the nucleus is intimate and in many cases the mitochondria may be found in shallow recesses with the nuclear envelope reflected over their surface. Subsequently the mitochondria became spheroid, reduced to five in number, and restricted to the sperm middle-piece. Two cylindrical centrioles are situated near the base of the nucleus and are surrounded by the mitochondria of the middle-piece. The distal centriole elongates and from it the longitudinal fibrils of the sperm flagellum originate. The proximal centriole, oriented obliquely to the distal centriole, retains its initial structure. Tests with Sudan black B showed the presence of phospholipids in the mitochondria of late spermatids and mature sperm. Cytochemical techniques for carbohydrates indicated a neutral mucoprotein for the proacrosomal vesicles and allowed these to be identified in early spermatogenesis. The development of the Mytilus spermatid was compared with that of other vertebrate… Advisors/Committee Members: Dornfeld, E. J. (advisor).

Subjects/Keywords: Spermatogenesis

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Longo, Frank J., 1. (1967). The fine structure of spermatid differentiation in the mussel, Mytilus edulis, Linn. (Doctoral Dissertation). Oregon State University. Retrieved from http://hdl.handle.net/1957/47217

Chicago Manual of Style (16^th Edition):

Longo, Frank J., 1939-. “The fine structure of spermatid differentiation in the mussel, Mytilus edulis, Linn.” 1967. Doctoral Dissertation, Oregon State University. Accessed March 07, 2021. http://hdl.handle.net/1957/47217.

MLA Handbook (7^th Edition):

Longo, Frank J., 1939-. “The fine structure of spermatid differentiation in the mussel, Mytilus edulis, Linn.” 1967. Web. 07 Mar 2021.

Vancouver:

Longo, Frank J. 1. The fine structure of spermatid differentiation in the mussel, Mytilus edulis, Linn. [Internet] [Doctoral dissertation]. Oregon State University; 1967. [cited 2021 Mar 07]. Available from: http://hdl.handle.net/1957/47217.

Council of Science Editors:

Longo, Frank J. 1. The fine structure of spermatid differentiation in the mussel, Mytilus edulis, Linn. [Doctoral Dissertation]. Oregon State University; 1967. Available from: http://hdl.handle.net/1957/47217

Oregon State University

3. Lin, Samuel. A study of the effect of 1,2-alpha-methlene-6-chloro-delta-4, 6-17-alpha-hydroxy progesterone acetate on the adult male rat including changes in the cell associations of the germinal epithelium.

Degree: MS, Zoology, 1967, Oregon State University

URL: http://hdl.handle.net/1957/47175

► SH 714 (1, 2-alpha-methylene-6-chloro-delta-4, 6-17-alphahydroxyprogesterone acetate) synthesized by Schering AG is an highly antiandrogenic, nonestrogenic compound. The present investigation was undertaken to determine the compound's… (more)

Subjects/Keywords: Spermatogenesis

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Lin, S. (1967). A study of the effect of 1,2-alpha-methlene-6-chloro-delta-4, 6-17-alpha-hydroxy progesterone acetate on the adult male rat including changes in the cell associations of the germinal epithelium. (Masters Thesis). Oregon State University. Retrieved from http://hdl.handle.net/1957/47175

Chicago Manual of Style (16^th Edition):

Lin, Samuel. “A study of the effect of 1,2-alpha-methlene-6-chloro-delta-4, 6-17-alpha-hydroxy progesterone acetate on the adult male rat including changes in the cell associations of the germinal epithelium.” 1967. Masters Thesis, Oregon State University. Accessed March 07, 2021. http://hdl.handle.net/1957/47175.

MLA Handbook (7^th Edition):

Lin, Samuel. “A study of the effect of 1,2-alpha-methlene-6-chloro-delta-4, 6-17-alpha-hydroxy progesterone acetate on the adult male rat including changes in the cell associations of the germinal epithelium.” 1967. Web. 07 Mar 2021.

Vancouver:

Lin S. A study of the effect of 1,2-alpha-methlene-6-chloro-delta-4, 6-17-alpha-hydroxy progesterone acetate on the adult male rat including changes in the cell associations of the germinal epithelium. [Internet] [Masters thesis]. Oregon State University; 1967. [cited 2021 Mar 07]. Available from: http://hdl.handle.net/1957/47175.

Council of Science Editors:

Lin S. A study of the effect of 1,2-alpha-methlene-6-chloro-delta-4, 6-17-alpha-hydroxy progesterone acetate on the adult male rat including changes in the cell associations of the germinal epithelium. [Masters Thesis]. Oregon State University; 1967. Available from: http://hdl.handle.net/1957/47175

Michigan State University

4. Cattani, Carola Marietta. Preferential segregation of the Y chromosome in spermatogenesis of Drosophila melanogaster.

Degree: MS, Department of Zoology, 1966, Michigan State University

URL: http://etd.lib.msu.edu/islandora/object/etd:40457

Subjects/Keywords: Spermatogenesis

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Cattani, C. M. (1966). Preferential segregation of the Y chromosome in spermatogenesis of Drosophila melanogaster. (Masters Thesis). Michigan State University. Retrieved from http://etd.lib.msu.edu/islandora/object/etd:40457

Chicago Manual of Style (16^th Edition):

Cattani, Carola Marietta. “Preferential segregation of the Y chromosome in spermatogenesis of Drosophila melanogaster.” 1966. Masters Thesis, Michigan State University. Accessed March 07, 2021. http://etd.lib.msu.edu/islandora/object/etd:40457.

MLA Handbook (7^th Edition):

Cattani, Carola Marietta. “Preferential segregation of the Y chromosome in spermatogenesis of Drosophila melanogaster.” 1966. Web. 07 Mar 2021.

Vancouver:

Cattani CM. Preferential segregation of the Y chromosome in spermatogenesis of Drosophila melanogaster. [Internet] [Masters thesis]. Michigan State University; 1966. [cited 2021 Mar 07]. Available from: http://etd.lib.msu.edu/islandora/object/etd:40457.

Council of Science Editors:

Cattani CM. Preferential segregation of the Y chromosome in spermatogenesis of Drosophila melanogaster. [Masters Thesis]. Michigan State University; 1966. Available from: http://etd.lib.msu.edu/islandora/object/etd:40457

Texas Tech University

5. Smith, Fannie E. Human spermatogenesis: a study of the spermatogonial population and ultrastructural aspects of spermatozoal development.

Degree: Biology, 1976, Texas Tech University

URL: http://hdl.handle.net/2346/10908

Subjects/Keywords: Spermatogenesis

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Smith, F. E. (1976). Human spermatogenesis: a study of the spermatogonial population and ultrastructural aspects of spermatozoal development. (Thesis). Texas Tech University. Retrieved from http://hdl.handle.net/2346/10908

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Smith, Fannie E. “Human spermatogenesis: a study of the spermatogonial population and ultrastructural aspects of spermatozoal development.” 1976. Thesis, Texas Tech University. Accessed March 07, 2021. http://hdl.handle.net/2346/10908.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Smith, Fannie E. “Human spermatogenesis: a study of the spermatogonial population and ultrastructural aspects of spermatozoal development.” 1976. Web. 07 Mar 2021.

Vancouver:

Smith FE. Human spermatogenesis: a study of the spermatogonial population and ultrastructural aspects of spermatozoal development. [Internet] [Thesis]. Texas Tech University; 1976. [cited 2021 Mar 07]. Available from: http://hdl.handle.net/2346/10908.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Smith FE. Human spermatogenesis: a study of the spermatogonial population and ultrastructural aspects of spermatozoal development. [Thesis]. Texas Tech University; 1976. Available from: http://hdl.handle.net/2346/10908

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Hong Kong

6. 楊善如. Regulation and functional studies of the testis-specific temperature-related sequence 4 (TRS4) in spermatogenesis.

Degree: 2014, University of Hong Kong

URL: http://hdl.handle.net/10722/208024

► Temperature-related sequence 4 (Trs4) is a transcript identified from the testis of a cryptorchid rat model. TRS4 is firstly detected in round spermatids on postnatal… (more)

▼ Temperature-related sequence 4 (Trs4) is a transcript identified from the testis of a cryptorchid rat model. TRS4 is firstly detected in round spermatids on postnatal day 21 mice, and is localized on the acrosome and tails of elongating spermatids and mature spermatozoa, suggesting a role in spermatogenesis. The present study aimed to investigate the regulatory and functional roles of Trs4 in spermatogenesis. To investigate the hormonal regulation of Trs4 in vitro, mouse germ cell were co-cultured with Sertoli (TM4) cell-line for 24 hours with 1μM or 10nM testosterone and the expression of Trs4 was quantitated. It was found that testosterone treatment at physiological level (10nM) significantly down-regulated Trs4 mRNA expression. However, TRS4 protein level did not change significantly. To investigate the functional roles of Trs4 and its interacting proteins (Rshl2, Gstmu1 and Ddc8) in spermatogenesis, the scrotal heat-treated rat model was used. The expression of Trs4, Rshl2, Gstmu1 and Ddc8 transcript were investigated on day 1, 9, 25, 37, 46, 56 and 79 post-heat treatment. Trs4, Rshl2 and Ddc8 transcripts were down-regulated on day 9 after heat-treatment, and recovered from day 37 onward. Immunohistochemical staining of TRS4 and RSHL-2 proteins showed they specifically localized in the cytoplasm of elongating spermatids in rat seminiferous tubules, suggesting that they might interact in regulating the development of elongating spermatids. Ten sessions of electroacupuncture (EA) was applied to the rats on day 9-36 to study the effect of EA in spermatogenesis. It was found that EA did not have any effect on the expression of Trs4, Rshl-2 and Ddc8 during recovery from hyperthermia when compared to the heat-treated only group. However, qPCR analysis on Gstmu1 mRNA expression showed a significant decrease in expression on day 46, and consistent with our TUNEL assay indicating a decrease in apoptosis in the EA group. Therefore, it is possible that Trs4 interacts with Gstmu1 and acts as anti-apoptotic factor for cell survival. To understand functional roles of Trs4 in mouse spermatogenesis, five Trs4 chimeric mice were generated in our laboratory and germline transmissible 〖Trs4〗^(flox/+) mice were identified. Sycp1-Cre mice were used to breed with Trs4 floxed mice to generate Trs4 floxed mice expressing Cre recombinase in spermatocytes (〖Trs4〗^(flox/+);〖Sycp-Cre〗^(+/0)) and aiming to generate Trs4 heterozygous KO mouse. However, after screening 74 offspring, no Trs4 heterozygous KO mouse was identified in this study and was likely to be caused by the low efficiency of recombination in Sycp1-Cre mice. Then, Zp3-Cre mice that expressing Cre-recombinase in oocyte were employed. Crossing of 〖Trs4〗^(flox/+):Zp3-〖Cre〗^(+/0) mice and Trs4 floxed mice could not produce heterozygous or homozygous Trs4 KO mouse, but suggested an aberrant recombination process happened in vivo. PCR assay confirmed DNA rearrangement occurred in regions flanking the exon 4 to the second LoxP site in Trs4 floxed mice. Furthermore, the…

Subjects/Keywords: Spermatogenesis

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

楊善如. (2014). Regulation and functional studies of the testis-specific temperature-related sequence 4 (TRS4) in spermatogenesis. (Thesis). University of Hong Kong. Retrieved from http://hdl.handle.net/10722/208024

Note: this citation may be lacking information needed for this citation format:
Author name may be incomplete
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

楊善如. “Regulation and functional studies of the testis-specific temperature-related sequence 4 (TRS4) in spermatogenesis.” 2014. Thesis, University of Hong Kong. Accessed March 07, 2021. http://hdl.handle.net/10722/208024.

Note: this citation may be lacking information needed for this citation format:
Author name may be incomplete
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

楊善如. “Regulation and functional studies of the testis-specific temperature-related sequence 4 (TRS4) in spermatogenesis.” 2014. Web. 07 Mar 2021.

Note: this citation may be lacking information needed for this citation format:
Author name may be incomplete

Vancouver:

楊善如. Regulation and functional studies of the testis-specific temperature-related sequence 4 (TRS4) in spermatogenesis. [Internet] [Thesis]. University of Hong Kong; 2014. [cited 2021 Mar 07]. Available from: http://hdl.handle.net/10722/208024.

Note: this citation may be lacking information needed for this citation format:
Author name may be incomplete
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

楊善如. Regulation and functional studies of the testis-specific temperature-related sequence 4 (TRS4) in spermatogenesis. [Thesis]. University of Hong Kong; 2014. Available from: http://hdl.handle.net/10722/208024

Note: this citation may be lacking information needed for this citation format:
Author name may be incomplete
Not specified: Masters Thesis or Doctoral Dissertation

Cornell University

7. Sartain, Caroline. Rna Regulation During Gametogenesis And Triggers Of Egg Activation In Drosophila Melanogaster.

Degree: PhD, Genetics, 2013, Cornell University

URL: http://hdl.handle.net/1813/34218

► One of the most critical transitory periods during development occurs when an oocyte prepares to undergo embryogenesis, in a process called "egg activation". In many… (more)

▼ One of the most critical transitory periods during development occurs when an oocyte prepares to undergo embryogenesis, in a process called "egg activation". In many organisms, the oocyte has been at rest in the ovary, stored in a dormant state for a period ranging from a number of days (as in flies) to several decades (as in humans). Upon egg activation, this dormant egg must suddenly undergo several major cellular and molecular changes that prepare it for embryogenesis, including restructuring of the vitelline membrane, completion of meiosis, and changes to mRNA and protein pools. Where most organisms require a fertilizing sperm to set off these events, fruitflies are unique in that egg activation occurs prior to requirement of fertilization. I have used Drosophila melanogaster as a model system to dissect apart some key events of egg activation that are driven solely by the maternal components of the oocyte. In most animals, the initial trigger from the sperm sparks a calcium wave that traverses through the oocyte, acting as a message to kick off previously silenced biochemical pathways. For many years, it was unknown whether calcium played a role in egg activation in Drosophila. I have discovered that although Drosophila oocytes do not require a fertilizing sperm, a calcium wave does occur during egg activation. I have characterized the dynamics of this calcium influx using live imaging of Drosophila oocytes undergoing in vitro egg activation. Furthermore, I found that the calcium flux is not dependent upon internal stores but depends on extracellular calcium concentrations. Additionally, I have determined that the extracellular calcium likely enters the oocyte through mechanosensory TRP channels. Downstream of the calcium signal, there is major turnover of the maternallystored mRNAs within the oocyte. I have examined the phenotype and transcriptome changes in eggs from mothers carrying the prage mutation, an allele previously uncharacterized in egg activation. I have also performed microarray analysis of the global set of transcripts that undergo cytoplasmic polyadenylation by WISPY at egg activation. These data taken together give us a big-picture view of the global changes occurring in the mRNA pool during egg activation. Advisors/Committee Members: Wolfner, Mariana Federica (chair), Goldberg, Michael Lewis (committee member), Liu, Jun (committee member).

Subjects/Keywords: Drosophila; egg activation; spermatogenesis

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Sartain, C. (2013). Rna Regulation During Gametogenesis And Triggers Of Egg Activation In Drosophila Melanogaster. (Doctoral Dissertation). Cornell University. Retrieved from http://hdl.handle.net/1813/34218

Chicago Manual of Style (16^th Edition):

Sartain, Caroline. “Rna Regulation During Gametogenesis And Triggers Of Egg Activation In Drosophila Melanogaster.” 2013. Doctoral Dissertation, Cornell University. Accessed March 07, 2021. http://hdl.handle.net/1813/34218.

MLA Handbook (7^th Edition):

Sartain, Caroline. “Rna Regulation During Gametogenesis And Triggers Of Egg Activation In Drosophila Melanogaster.” 2013. Web. 07 Mar 2021.

Vancouver:

Sartain C. Rna Regulation During Gametogenesis And Triggers Of Egg Activation In Drosophila Melanogaster. [Internet] [Doctoral dissertation]. Cornell University; 2013. [cited 2021 Mar 07]. Available from: http://hdl.handle.net/1813/34218.

Council of Science Editors:

Sartain C. Rna Regulation During Gametogenesis And Triggers Of Egg Activation In Drosophila Melanogaster. [Doctoral Dissertation]. Cornell University; 2013. Available from: http://hdl.handle.net/1813/34218

University of Newcastle

8. Lv , Yonggang. Study the male reproductive biology of a marine invertebrate Galeolaria caespitosa and investigate its potential as a bio-indicator species of coastal marine pollution.

Degree: PhD, 2016, University of Newcastle

URL: http://hdl.handle.net/1959.13/1332731

►

Research Doctorate - Doctor of Philosophy (PhD)

The history of marine pollution can be traced back to the very beginning of human civilisation. Nevertheless, this… (more)

▼

Research Doctorate - Doctor of Philosophy (PhD)

The history of marine pollution can be traced back to the very beginning of human civilisation. Nevertheless, this issue did not raise serious concern until a threshold level was reached with devastating consequences for the marine ecosystem. In recent years, bio-indicator species have been gradually embraced as one of the most effective approaches to predict, monitor and assess marine contamination. The statistics acquired from biomonitoring not only facilitate determination of the biological significance of man-made hazardous substances on marine ecosystems but also establish an important foundation for marine wildlife conservation and public health management. The focal species in this PhD project is an Australian native marine invertebrate Galeolaria caespitosa, which is recognised as a potential bio-indicator species for coastal marine pollution and a model organism for use in laboratory toxicity tests, due to a number of remarkable life history characteristics it exhibits. According to a range of recent studies, the gametes of G. caespitosa, particularly its spermatozoa, and the processes of fertilisation, embryogenesis and larval development were susceptible to marine contaminants and could be utilised as sensitive indicators of pollutants in the immediate environment. Nevertheless, the reproductive biology of this species, particular the male reproductive system, has only been superficially described. This knowledge gap has created an opportunity to undertake a comprehensive investigation of reproduction and embryonic development in this species including the sensitivity of these processes to common environmental contaminants. (1) Utilising serial histological sections and electron microscopic techniques, the male reproductive system in G. caespitosa was reconstructed and the general pattern of spermatogenesis was established. This study for the first time established a comprehensive model of male reproductive system in polychaetes, with the function of each compartment being elucidated in detail. In addition, clear similarities were found between the male reproductive system in this simple invertebrate and human beings in terms of the structure and function.(2) As the differentiation of round spermatids in G. caespitosa took place in the germinal fluid without any physical support from nurse cells, spermiogenesis in this species was regarded as a convenient model for studying the underlying mechanisms of this complex differentiation process. Therefore, spermiogenesis was analysed in detail at the ultrastructural level and a preliminary study was performed to reproduce the differentiation of spermatids in vitro. This in vitro study revealed that the regulators of spermiogenesis in G. caespitosa were gender-specific and involved proteinaceous constituents thereby providing a basis for future studies addressing the molecular regulation of this complex differentiation process. (3) Exposure of spermatozoa to low…

Advisors/Committee Members: University of Newcastle. Faculty of Science & Information Technology, School of Environmental and Life Sciences.

Subjects/Keywords: spermatogenesis; embryogenesis; plasticizer; bio-indicator

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Lv , Y. (2016). Study the male reproductive biology of a marine invertebrate Galeolaria caespitosa and investigate its potential as a bio-indicator species of coastal marine pollution. (Doctoral Dissertation). University of Newcastle. Retrieved from http://hdl.handle.net/1959.13/1332731

Chicago Manual of Style (16^th Edition):

Lv , Yonggang. “Study the male reproductive biology of a marine invertebrate Galeolaria caespitosa and investigate its potential as a bio-indicator species of coastal marine pollution.” 2016. Doctoral Dissertation, University of Newcastle. Accessed March 07, 2021. http://hdl.handle.net/1959.13/1332731.

MLA Handbook (7^th Edition):

Lv , Yonggang. “Study the male reproductive biology of a marine invertebrate Galeolaria caespitosa and investigate its potential as a bio-indicator species of coastal marine pollution.” 2016. Web. 07 Mar 2021.

Vancouver:

Lv Y. Study the male reproductive biology of a marine invertebrate Galeolaria caespitosa and investigate its potential as a bio-indicator species of coastal marine pollution. [Internet] [Doctoral dissertation]. University of Newcastle; 2016. [cited 2021 Mar 07]. Available from: http://hdl.handle.net/1959.13/1332731.

Council of Science Editors:

Lv Y. Study the male reproductive biology of a marine invertebrate Galeolaria caespitosa and investigate its potential as a bio-indicator species of coastal marine pollution. [Doctoral Dissertation]. University of Newcastle; 2016. Available from: http://hdl.handle.net/1959.13/1332731

University of Nairobi

9. Oduma, J.A. Effects of Ripe Carica Papaya Seed Powder on Testicular Histology of Boars .

Degree: 2012, University of Nairobi

URL: http://erepository.uonbi.ac.ke:8080/xmlui/handle/123456789/10910

► The effect of oral administration of ripe Carica Papaya seed powder on testicular histology of Sus scrofa domestica boars has been addressed in the present… (more)

Subjects/Keywords: Pawpaw seeds; Spermatogenesis; Testicular histology

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Oduma, J. A. (2012). Effects of Ripe Carica Papaya Seed Powder on Testicular Histology of Boars . (Thesis). University of Nairobi. Retrieved from http://erepository.uonbi.ac.ke:8080/xmlui/handle/123456789/10910

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Oduma, J A. “Effects of Ripe Carica Papaya Seed Powder on Testicular Histology of Boars .” 2012. Thesis, University of Nairobi. Accessed March 07, 2021. http://erepository.uonbi.ac.ke:8080/xmlui/handle/123456789/10910.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Oduma, J A. “Effects of Ripe Carica Papaya Seed Powder on Testicular Histology of Boars .” 2012. Web. 07 Mar 2021.

Vancouver:

Oduma JA. Effects of Ripe Carica Papaya Seed Powder on Testicular Histology of Boars . [Internet] [Thesis]. University of Nairobi; 2012. [cited 2021 Mar 07]. Available from: http://erepository.uonbi.ac.ke:8080/xmlui/handle/123456789/10910.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Oduma JA. Effects of Ripe Carica Papaya Seed Powder on Testicular Histology of Boars . [Thesis]. University of Nairobi; 2012. Available from: http://erepository.uonbi.ac.ke:8080/xmlui/handle/123456789/10910

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Guelph

10. Giffin, Jennifer L. Determinants of Testicular Echotexture in the Sexually Immature Ram Lamb.

Degree: PhD, Department of Biomedical Sciences, 2015, University of Guelph

URL: https://atrium.lib.uoguelph.ca/xmlui/handle/10214/8662

► Throughout sexual maturation, dynamic changes in testicular macro- and microstructure and reproductive hormone levels occur. Future adult reproductive capability is critically dependent on these changes;… (more)

▼ Throughout sexual maturation, dynamic changes in testicular macro- and microstructure and reproductive hormone levels occur. Future adult reproductive capability is critically dependent on these changes; therefore, regular monitoring of pubertal testicular development is desirable. However, conventional methods of assessment do not permit the frequent and non-invasive examination of testicular function. Recently, scrotal ultrasonography in conjunction with computer-assisted image analysis has emerged as a potential non-invasive alternative for male reproductive assessment. In this procedure, testicular echotexture, or the appearance of the ultrasonogram, is objectively quantified on the basis of brightness or intensity of the minute picture elements, or pixels, comprising the image. In general, testicular pixel intensity increases with age throughout sexual maturation; however, periodic fluctuations occur. Changes in testicular echotexture are related to microstructural attributes of the testes and reproductive hormone secretion, but reports on these relationships have been inconsistent. Therefore, the overall objective of the studies presented in this thesis was to investigate how testicular echotexture and its associations with testicular histomorphology and endocrine profiles may be influenced by various factors including: i) scrotal/testicular integument; ii) blood flow/content; iii) stage of development; and iv) altered spermatogenic onset. Serial scrotal ultrasonograms, testicular biopsies or castrations and blood samples were collected from early postnatal or peripubescent ram lambs and analyzed to obtain echotextural, histomorphological and endocrine data, respectively. Testicular echotextural attributes were influenced by the presence of the scrotal and testicular integument, the stage of spermatogenic development (mitotic vs. post-mitotic) and the amount of germ cell heterogeneity present with or without retinoic acid exposure, but not by blood flow or content. Tubular and luminal size of the seminiferous tubules could be predicted from testicular pixel intensity using a simple linear regression model and demonstrated a moderate strength correlation with testicular echotextural parameters, as did a number of cellular and sub-cellular histomorphological attributes and reproductive hormone levels. These results clearly demonstrate the ability of testicular echotexture to detect experimentally and clinically relevant markers of testicular function. Computerized image analysis of scrotal ultrasonograms holds promise as a non-invasive diagnostic and prognostic tool and novel monitoring device of testicular histophysiology. Advisors/Committee Members: Bartlewski, Pawel M. (advisor).

Subjects/Keywords: testis; ultrasound; puberty; spermatogenesis

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Giffin, J. L. (2015). Determinants of Testicular Echotexture in the Sexually Immature Ram Lamb. (Doctoral Dissertation). University of Guelph. Retrieved from https://atrium.lib.uoguelph.ca/xmlui/handle/10214/8662

Chicago Manual of Style (16^th Edition):

Giffin, Jennifer L. “Determinants of Testicular Echotexture in the Sexually Immature Ram Lamb.” 2015. Doctoral Dissertation, University of Guelph. Accessed March 07, 2021. https://atrium.lib.uoguelph.ca/xmlui/handle/10214/8662.

MLA Handbook (7^th Edition):

Giffin, Jennifer L. “Determinants of Testicular Echotexture in the Sexually Immature Ram Lamb.” 2015. Web. 07 Mar 2021.

Vancouver:

Giffin JL. Determinants of Testicular Echotexture in the Sexually Immature Ram Lamb. [Internet] [Doctoral dissertation]. University of Guelph; 2015. [cited 2021 Mar 07]. Available from: https://atrium.lib.uoguelph.ca/xmlui/handle/10214/8662.

Council of Science Editors:

Giffin JL. Determinants of Testicular Echotexture in the Sexually Immature Ram Lamb. [Doctoral Dissertation]. University of Guelph; 2015. Available from: https://atrium.lib.uoguelph.ca/xmlui/handle/10214/8662

11. Ruggiu, Matteo. The function of DAZL and RBM, two candidate fertility genes.

Degree: PhD, 1999, Open University

URL: https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.300014

Subjects/Keywords: 572.8; Spermatogenesis

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Ruggiu, M. (1999). The function of DAZL and RBM, two candidate fertility genes. (Doctoral Dissertation). Open University. Retrieved from https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.300014

Chicago Manual of Style (16^th Edition):

Ruggiu, Matteo. “The function of DAZL and RBM, two candidate fertility genes.” 1999. Doctoral Dissertation, Open University. Accessed March 07, 2021. https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.300014.

MLA Handbook (7^th Edition):

Ruggiu, Matteo. “The function of DAZL and RBM, two candidate fertility genes.” 1999. Web. 07 Mar 2021.

Vancouver:

Ruggiu M. The function of DAZL and RBM, two candidate fertility genes. [Internet] [Doctoral dissertation]. Open University; 1999. [cited 2021 Mar 07]. Available from: https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.300014.

Council of Science Editors:

Ruggiu M. The function of DAZL and RBM, two candidate fertility genes. [Doctoral Dissertation]. Open University; 1999. Available from: https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.300014

12. Grootegoed, Anton. Hormones and the control of biochemical processes in spermatogenesis.

Degree: 1978, Erasmus University Medical Center

URL: http://hdl.handle.net/1765/25954

► textabstractThe aim of the experiments described in this thesis was to contribute to a better understanding of the relationship, in molecular terms, between hormones and… (more)

Subjects/Keywords: hormones; spermatogenesis

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Grootegoed, A. (1978). Hormones and the control of biochemical processes in spermatogenesis. (Doctoral Dissertation). Erasmus University Medical Center. Retrieved from http://hdl.handle.net/1765/25954

Chicago Manual of Style (16^th Edition):

Grootegoed, Anton. “Hormones and the control of biochemical processes in spermatogenesis.” 1978. Doctoral Dissertation, Erasmus University Medical Center. Accessed March 07, 2021. http://hdl.handle.net/1765/25954.

MLA Handbook (7^th Edition):

Grootegoed, Anton. “Hormones and the control of biochemical processes in spermatogenesis.” 1978. Web. 07 Mar 2021.

Vancouver:

Grootegoed A. Hormones and the control of biochemical processes in spermatogenesis. [Internet] [Doctoral dissertation]. Erasmus University Medical Center; 1978. [cited 2021 Mar 07]. Available from: http://hdl.handle.net/1765/25954.

Council of Science Editors:

Grootegoed A. Hormones and the control of biochemical processes in spermatogenesis. [Doctoral Dissertation]. Erasmus University Medical Center; 1978. Available from: http://hdl.handle.net/1765/25954

Texas Tech University

13. McIlroy, Anne Salman. Spermatogenesis of Bufo woodhousei: a thesis in zoology.

Degree: Zoology, 1955, Texas Tech University

URL: http://hdl.handle.net/2346/8582

► Por a number of years the spermatogenesis of amphibians has been of interest to zoologists because (1) these are the first vertebrates to have emerged… (more)

Subjects/Keywords: Spermatogenesis; Bufo

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

McIlroy, A. S. (1955). Spermatogenesis of Bufo woodhousei: a thesis in zoology. (Thesis). Texas Tech University. Retrieved from http://hdl.handle.net/2346/8582

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

McIlroy, Anne Salman. “Spermatogenesis of Bufo woodhousei: a thesis in zoology.” 1955. Thesis, Texas Tech University. Accessed March 07, 2021. http://hdl.handle.net/2346/8582.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

McIlroy, Anne Salman. “Spermatogenesis of Bufo woodhousei: a thesis in zoology.” 1955. Web. 07 Mar 2021.

Vancouver:

McIlroy AS. Spermatogenesis of Bufo woodhousei: a thesis in zoology. [Internet] [Thesis]. Texas Tech University; 1955. [cited 2021 Mar 07]. Available from: http://hdl.handle.net/2346/8582.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

McIlroy AS. Spermatogenesis of Bufo woodhousei: a thesis in zoology. [Thesis]. Texas Tech University; 1955. Available from: http://hdl.handle.net/2346/8582

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Texas Tech University

14. McIlroy, Anne Salman. Spermatogenesis of Bufo woodhousei: a thesis in zoology.

Degree: Zoology, 1955, Texas Tech University

URL: http://hdl.handle.net/2346/20780

Subjects/Keywords: Bufo; Spermatogenesis

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

McIlroy, A. S. (1955). Spermatogenesis of Bufo woodhousei: a thesis in zoology. (Thesis). Texas Tech University. Retrieved from http://hdl.handle.net/2346/20780

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

McIlroy, Anne Salman. “Spermatogenesis of Bufo woodhousei: a thesis in zoology.” 1955. Thesis, Texas Tech University. Accessed March 07, 2021. http://hdl.handle.net/2346/20780.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

McIlroy, Anne Salman. “Spermatogenesis of Bufo woodhousei: a thesis in zoology.” 1955. Web. 07 Mar 2021.

Vancouver:

McIlroy AS. Spermatogenesis of Bufo woodhousei: a thesis in zoology. [Internet] [Thesis]. Texas Tech University; 1955. [cited 2021 Mar 07]. Available from: http://hdl.handle.net/2346/20780.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

McIlroy AS. Spermatogenesis of Bufo woodhousei: a thesis in zoology. [Thesis]. Texas Tech University; 1955. Available from: http://hdl.handle.net/2346/20780

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Hong Kong

15. Tang, Elizabeth Ingrid Yingyu. Regulation of the cytoskeleton during spermatogenesis.

Degree: 2016, University of Hong Kong

URL: http://hdl.handle.net/10722/238350

► Spermatogenesis is a complex process that takes place in the seminiferous tubules of the mammalian testes to produce spermatozoa. The epithelium of the seminiferous tubules… (more)

▼ Spermatogenesis is a complex process that takes place in the seminiferous tubules of the mammalian testes to produce spermatozoa. The epithelium of the seminiferous tubules is comprised of germ cells (GC), at different stages of development, and Sertoli cells (SC). The SC has the task of creating the blood-testis barrier (BTB), providing nutritional support to GCs, and supporting GC transport across the BTB and epithelium. As the SC is involved in such critical and dynamic processes, it is conceivable that SCs are tightly regulated in order to ensure the cellular events of spermatogenesis are carried out flawlessly. A dynamic cytoskeleton enables the SC to do this. Of the three major classes of filamentous proteins in the SC cytoskeleton: actin filaments, intermediate filaments, and microtubules (MT), actin filaments are the best-studied component in SCs. However, the cytoskeleton cannot be fully understood by looking at its individual components, because they all work together to form the cytoskeleton. Researchers across different fields have found that the relationship between actin filaments and MTs is greater than originally thought. The current understanding of SC MTs is that they are essential for structural support of the cell and providing tracks for GC transport across the epithelium, but, beyond this function, little is known about their regulation and interaction with other cytoskeleton components. Herein it is proposed that SC MTs are involved in regulating the ectoplasmic specialization (ES) and BTB, which are actin-rich cell junction sites within the seminiferous epithelium, as well as transport during spermatogenesis. The studies presented here aim to address the regulation of SC MTs during spermatogenesis. The functions of MT regulatory proteins: MAP/MT affinity-regulating kinases 2 and 4 (MARK2, MARK4) and end-binding protein 1 (EB1), were investigated. MARK2 and MARK4 were shown to be involved in maintenance of the ES and BTB by regulating MTs. Knockdown of MARK2 versus MARK4 in vitro by RNAi in cultured rat SCs revealed that the isoforms differentially regulated the SC ES and BTB. Loss of MARK2 function led to perturbation of TJ-permeability barrier via disruption of TJ protein distribution. Similarly, MARK4 knockdown perturbed the TJ-permeability barrier, however effects were more pronounced than in MARK2 RNAi cells, as its knockdown led to mislocalization of basal ES and TJ proteins. Knockdown of EB1 also perturbed the TJ-permeability barrier by causing mislocalization of junction proteins. Reduction of either MARK2, MARK4, or EB1 disrupted MT distribution throughout SCs, leading to mislocalization of junction proteins. Additionally, it was discovered that alterations in MT arrangement in SCs affected the actin cytoskeleton, which directly supports BTB function. Following these studies, the adjudin model was used to relate these findings to in vivo conditions. Adjudin is a potential male contraceptive and disrupts actin-based adhesion to cause premature GC/spermatid loss. Despite actin…

Subjects/Keywords: Cytoskeleton; Spermatogenesis

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Tang, E. I. Y. (2016). Regulation of the cytoskeleton during spermatogenesis. (Thesis). University of Hong Kong. Retrieved from http://hdl.handle.net/10722/238350

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Tang, Elizabeth Ingrid Yingyu. “Regulation of the cytoskeleton during spermatogenesis.” 2016. Thesis, University of Hong Kong. Accessed March 07, 2021. http://hdl.handle.net/10722/238350.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Tang, Elizabeth Ingrid Yingyu. “Regulation of the cytoskeleton during spermatogenesis.” 2016. Web. 07 Mar 2021.

Vancouver:

Tang EIY. Regulation of the cytoskeleton during spermatogenesis. [Internet] [Thesis]. University of Hong Kong; 2016. [cited 2021 Mar 07]. Available from: http://hdl.handle.net/10722/238350.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Tang EIY. Regulation of the cytoskeleton during spermatogenesis. [Thesis]. University of Hong Kong; 2016. Available from: http://hdl.handle.net/10722/238350

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Texas – Austin

16. McKee, Chad Marcus, 1975-. The modulation of apoptosis in testicular germ cells following toxicant-induced cellular stress.

Degree: PhD, Cell and Molecular Biology, 2007, University of Texas – Austin

URL: http://hdl.handle.net/2152/3633

► Di-(2-ethylhexyl) phthalate (DEHP) is a ubiquitous environmental toxicant. The active metabolite of DEHP, mono-(2-ethylhexyl) phthalate (MEHP), is ultimately responsible for disrupting the process of spermatogenesis… (more)

▼ Di-(2-ethylhexyl) phthalate (DEHP) is a ubiquitous environmental toxicant. The active metabolite of DEHP, mono-(2-ethylhexyl) phthalate (MEHP), is ultimately responsible for disrupting the process of spermatogenesis and promoting germ cell death. In addition, this toxicant has been positively correlated with developmental problems such as cryptorchidism, a derangement of the seminiferous tubules, and a syndrome called testicular dysgenesis, leading to reduced sperm number. The potential impact of MEHP on human fertility justifies a detailed investigation into the mechanisms by which this agent causes germ cell death. MEHP is known to directly target and damage the Sertoli cell, a testicular cell whose main function is to support the development of the principle germ cell types from the earliest stem cell to the most mature spermatozoa. This dissertation examines the downstream effect of Sertoli cell damage on germ cell homeostasis and the proteins that modulate the sensitivity of germ cells to undergo apoptotic elimination. Specifically, the stabilization of the p53 protein is proposed to be an important upstream determinant of Fas-mediated apoptosis in germ cells following MEHP exposure. Furthermore, that the resulting cell death is the result of increased death receptor expression and c-FLIPL ubiquitinylation. The mechanism is speculated to reside in the spermatocyte sub-population of germ cells, which appears to be most responsive to changes in apoptosis. Exposures of wild type mice to MEHP caused an increased p53 stability and elevated protein levels of the membrane-bound death receptors Fas and DR5 in testicular spermatocytes. The expression of these proteins occur coincident with increases in spermatocyte apoptosis and are driven by p53 activity. To further assess the mechanisms responsible for the sensitivity of germ cells to undergo p53-mediated apoptosis, we used the germ cell line GC-2spd(ts) (a p53 temperature sensitive spermatocyte-like cell line that allows for p53 nuclear localization at 32°C but not 37°C). Induction of the p53 protein led to higher levels of the death receptors DR5 and Fas, activation of caspase-8, and decreases in c-FLIPL. Addition of TRAIL (the cognate ligand for DR5) and the agonistic DR5 agonistic antibody MD5-1, triggered a robust synergistic increase of apoptosis in GC-2 cells maintained at the p53 permissive temperature (32°C). DR5 levels on the germ cell plasma membrane were considerably enhanced following these treatments. Immunoprecipitation of c-FLIPL suggests that the protein is ubiquitinylated after cellular stress and concomitant with p53 activity. Experiments also reveal that c-FLIPL levels may be influenced by Itch, a regulatory protein able to label targets for the proteasomal degradation using a ubiquitinylating E3 ligase. Immunohistochemical detection in adult wild type mouse testis show robust increases in Itch protein levels upon MEHP treatment (1g/kg) and subsequently localization to the cytoplasm of meiotic spermatocyte germ cells. Western blot analysis of testis… Advisors/Committee Members: Richburg, John H. (advisor).

Subjects/Keywords: Apoptosis; Spermatogenesis

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

McKee, Chad Marcus, 1. (2007). The modulation of apoptosis in testicular germ cells following toxicant-induced cellular stress. (Doctoral Dissertation). University of Texas – Austin. Retrieved from http://hdl.handle.net/2152/3633

Chicago Manual of Style (16^th Edition):

McKee, Chad Marcus, 1975-. “The modulation of apoptosis in testicular germ cells following toxicant-induced cellular stress.” 2007. Doctoral Dissertation, University of Texas – Austin. Accessed March 07, 2021. http://hdl.handle.net/2152/3633.

MLA Handbook (7^th Edition):

McKee, Chad Marcus, 1975-. “The modulation of apoptosis in testicular germ cells following toxicant-induced cellular stress.” 2007. Web. 07 Mar 2021.

Vancouver:

McKee, Chad Marcus 1. The modulation of apoptosis in testicular germ cells following toxicant-induced cellular stress. [Internet] [Doctoral dissertation]. University of Texas – Austin; 2007. [cited 2021 Mar 07]. Available from: http://hdl.handle.net/2152/3633.

Council of Science Editors:

McKee, Chad Marcus 1. The modulation of apoptosis in testicular germ cells following toxicant-induced cellular stress. [Doctoral Dissertation]. University of Texas – Austin; 2007. Available from: http://hdl.handle.net/2152/3633

Michigan State University

17. Poirier, Gary Raymond, 1938-. An ultrastructural study of spermatogenesis in two species of Rana.

Degree: PhD, Department of Zoology, 1970, Michigan State University

URL: http://etd.lib.msu.edu/islandora/object/etd:37231

Subjects/Keywords: Spermatogenesis; Frogs

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Poirier, Gary Raymond, 1. (1970). An ultrastructural study of spermatogenesis in two species of Rana. (Doctoral Dissertation). Michigan State University. Retrieved from http://etd.lib.msu.edu/islandora/object/etd:37231

Chicago Manual of Style (16^th Edition):

Poirier, Gary Raymond, 1938-. “An ultrastructural study of spermatogenesis in two species of Rana.” 1970. Doctoral Dissertation, Michigan State University. Accessed March 07, 2021. http://etd.lib.msu.edu/islandora/object/etd:37231.

MLA Handbook (7^th Edition):

Poirier, Gary Raymond, 1938-. “An ultrastructural study of spermatogenesis in two species of Rana.” 1970. Web. 07 Mar 2021.

Vancouver:

Poirier, Gary Raymond 1. An ultrastructural study of spermatogenesis in two species of Rana. [Internet] [Doctoral dissertation]. Michigan State University; 1970. [cited 2021 Mar 07]. Available from: http://etd.lib.msu.edu/islandora/object/etd:37231.

Council of Science Editors:

Poirier, Gary Raymond 1. An ultrastructural study of spermatogenesis in two species of Rana. [Doctoral Dissertation]. Michigan State University; 1970. Available from: http://etd.lib.msu.edu/islandora/object/etd:37231

Cape Peninsula University of Technology

18. Aboua, Yapo Guillaume. The impact of organic hydroperoxides and a red palm oil supplemented diet on spermatogenesis, sperm function and sperm apoptosis .

Degree: 2009, Cape Peninsula University of Technology

URL: http://etd.cput.ac.za/handle/20.500.11838/1523

► Many environmental, physiological, and genetic factors have been shown to impair sperm function through oxidative damage. Oxidative stress (OS) arises as a consequence of excessive… (more)

▼ Many environmental, physiological, and genetic factors have been shown to impair sperm function through oxidative damage. Oxidative stress (OS) arises as a consequence of excessive reactive oxygen species (ROS) production and/or impaired antioxidant defence mechanisms. The decline in male reproductive health generated considerable public and scientific concerns about the possible role of environmental contaminants. A better understanding of how OS affects sperm function will be beneficial as it might help in the design of new and effective treatment strategies to combat the problem of increasing male subfertility. Studies have suggested that antioxidant nutrients and/or medicines play a protective role in human health. Crude red palm oil (RPO) is known to be the richest natural plant source of antioxidants such as carotenoids, tocopherols and metalloporpheryns. The aims of this study were twofold: (i) To establish an in vivo animal model of OS by exposing rat to organic hydroperoxide such as t-butyl hydroperoxide (tbHP) and cumene hydroperoxide (cHP) through repeated intraperitoneal injections that can be used for studying these effects on testicular tissue, epididymal sperm and sperm function as well as male reproductive parameters in general. (ii) To investigate the effects of a RPO supplemented diet on male reproductive parameters and tissue in animals exposed to OS. In the first part of the study, male Wistar rats aged 10-12 weeks were randomly placed in groups and received standard rat chow (SRC) and water ad lib. Animals were injected intraperitoneally with saline (0.5 ml), t-butyl hydroperoxide (5µM, 10µM, 20µM and 40µM; 0.5 ml) or cumene hydroperoxide cHP (2.5µM, 5µM, 10µM and 20µM; 0.5 ml) over a 60 day period. In the second part, male Wistar rats aged 10-12 weeks were placed randomly in three groups and fed with SRC. Group 1 received no supplement while the food of groups 2 and 3 were supplemented with 2 mL and 4 mL RPO (in 25 gm SRC/day) respectively. Each group was further divided into 3 subgroups and injected intraperitoneally daily with either saline, 10µM cHP or 20µM tbHP respectively. This was done for 5 consecutive days per week over a 60 day period. Sperm concentrations, and motility, lipid peroxidation, superoxide dismutase (SOD), catalase (CAT) and glutathione (GSH) activities as well as apoptosis were assessed.

Subjects/Keywords: Spermatogenesis; Spermatozoa – Physiology; Palm oil; Peroxides

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Aboua, Y. G. (2009). The impact of organic hydroperoxides and a red palm oil supplemented diet on spermatogenesis, sperm function and sperm apoptosis . (Thesis). Cape Peninsula University of Technology. Retrieved from http://etd.cput.ac.za/handle/20.500.11838/1523

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Aboua, Yapo Guillaume. “The impact of organic hydroperoxides and a red palm oil supplemented diet on spermatogenesis, sperm function and sperm apoptosis .” 2009. Thesis, Cape Peninsula University of Technology. Accessed March 07, 2021. http://etd.cput.ac.za/handle/20.500.11838/1523.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Aboua, Yapo Guillaume. “The impact of organic hydroperoxides and a red palm oil supplemented diet on spermatogenesis, sperm function and sperm apoptosis .” 2009. Web. 07 Mar 2021.

Vancouver:

Aboua YG. The impact of organic hydroperoxides and a red palm oil supplemented diet on spermatogenesis, sperm function and sperm apoptosis . [Internet] [Thesis]. Cape Peninsula University of Technology; 2009. [cited 2021 Mar 07]. Available from: http://etd.cput.ac.za/handle/20.500.11838/1523.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Aboua YG. The impact of organic hydroperoxides and a red palm oil supplemented diet on spermatogenesis, sperm function and sperm apoptosis . [Thesis]. Cape Peninsula University of Technology; 2009. Available from: http://etd.cput.ac.za/handle/20.500.11838/1523

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Hong Kong

19. 左妍. Gene targeting to study a novel acrosome specific gene VAD1.3/AEP1 in spermatogenesis.

Degree: 2008, University of Hong Kong

URL: http://hdl.handle.net/10722/54422

Subjects/Keywords: Spermatogenesis.; Gene targeting.

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

左妍. (2008). Gene targeting to study a novel acrosome specific gene VAD1.3/AEP1 in spermatogenesis. (Thesis). University of Hong Kong. Retrieved from http://hdl.handle.net/10722/54422

Note: this citation may be lacking information needed for this citation format:
Author name may be incomplete
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

左妍. “Gene targeting to study a novel acrosome specific gene VAD1.3/AEP1 in spermatogenesis.” 2008. Thesis, University of Hong Kong. Accessed March 07, 2021. http://hdl.handle.net/10722/54422.

Note: this citation may be lacking information needed for this citation format:
Author name may be incomplete
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

左妍. “Gene targeting to study a novel acrosome specific gene VAD1.3/AEP1 in spermatogenesis.” 2008. Web. 07 Mar 2021.

Note: this citation may be lacking information needed for this citation format:
Author name may be incomplete

Vancouver:

左妍. Gene targeting to study a novel acrosome specific gene VAD1.3/AEP1 in spermatogenesis. [Internet] [Thesis]. University of Hong Kong; 2008. [cited 2021 Mar 07]. Available from: http://hdl.handle.net/10722/54422.

Note: this citation may be lacking information needed for this citation format:
Author name may be incomplete
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

左妍. Gene targeting to study a novel acrosome specific gene VAD1.3/AEP1 in spermatogenesis. [Thesis]. University of Hong Kong; 2008. Available from: http://hdl.handle.net/10722/54422

Note: this citation may be lacking information needed for this citation format:
Author name may be incomplete
Not specified: Masters Thesis or Doctoral Dissertation

University of Hong Kong

20. Li, Wing-man, Michelle. Gap junction regulates the blood-testis barrier dynamics during spermatogenesis.

Degree: 2010, University of Hong Kong

URL: http://hdl.handle.net/10722/132274

Subjects/Keywords: Spermatogenesis.; Cell junctions.

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Li, Wing-man, M. (2010). Gap junction regulates the blood-testis barrier dynamics during spermatogenesis. (Thesis). University of Hong Kong. Retrieved from http://hdl.handle.net/10722/132274

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Li, Wing-man, Michelle. “Gap junction regulates the blood-testis barrier dynamics during spermatogenesis.” 2010. Thesis, University of Hong Kong. Accessed March 07, 2021. http://hdl.handle.net/10722/132274.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Li, Wing-man, Michelle. “Gap junction regulates the blood-testis barrier dynamics during spermatogenesis.” 2010. Web. 07 Mar 2021.

Vancouver:

Li, Wing-man M. Gap junction regulates the blood-testis barrier dynamics during spermatogenesis. [Internet] [Thesis]. University of Hong Kong; 2010. [cited 2021 Mar 07]. Available from: http://hdl.handle.net/10722/132274.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Li, Wing-man M. Gap junction regulates the blood-testis barrier dynamics during spermatogenesis. [Thesis]. University of Hong Kong; 2010. Available from: http://hdl.handle.net/10722/132274

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Kashmir

21. Safaya, Phoola. Studies on Chromosomes of Kashmir fishes with their number structure and karyotypes in Spermatogenesis of twelve fishes;.

Degree: 2014, University of Kashmir

URL: http://shodhganga.inflibnet.ac.in/handle/10603/20413

newline

Advisors/Committee Members: Das, S. M..

Subjects/Keywords: Chromosomes-Spermatogenesis-Kashmir

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Safaya, P. (2014). Studies on Chromosomes of Kashmir fishes with their number structure and karyotypes in Spermatogenesis of twelve fishes;. (Thesis). University of Kashmir. Retrieved from http://shodhganga.inflibnet.ac.in/handle/10603/20413

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Safaya, Phoola. “Studies on Chromosomes of Kashmir fishes with their number structure and karyotypes in Spermatogenesis of twelve fishes;.” 2014. Thesis, University of Kashmir. Accessed March 07, 2021. http://shodhganga.inflibnet.ac.in/handle/10603/20413.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Safaya, Phoola. “Studies on Chromosomes of Kashmir fishes with their number structure and karyotypes in Spermatogenesis of twelve fishes;.” 2014. Web. 07 Mar 2021.

Vancouver:

Safaya P. Studies on Chromosomes of Kashmir fishes with their number structure and karyotypes in Spermatogenesis of twelve fishes;. [Internet] [Thesis]. University of Kashmir; 2014. [cited 2021 Mar 07]. Available from: http://shodhganga.inflibnet.ac.in/handle/10603/20413.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Safaya P. Studies on Chromosomes of Kashmir fishes with their number structure and karyotypes in Spermatogenesis of twelve fishes;. [Thesis]. University of Kashmir; 2014. Available from: http://shodhganga.inflibnet.ac.in/handle/10603/20413

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Texas A&M University

22. Heninger, Noah Leland, III. The role of testicular germ cell apoptosis during equine spermatogenesis.

Degree: PhD, Physiology of Reproduction, 2007, Texas A&M University

URL: http://hdl.handle.net/1969.1/4691

► Apoptosis in testicular germ cells has been demonstrated in many species. Features of apoptosis reported in other species were used to confirm use of the… (more)

▼ Apoptosis in testicular germ cells has been demonstrated in many species. Features of apoptosis reported in other species were used to confirm use of the TUNEL assay in stallion testes. Eight stallions with normal testicular size and semen quality were evaluated to determine the germ cell types and stages where apoptosis most commonly occurs. Mean numbers of TUNEL-positive germ cells per 100 Sertoli cell nuclei were highest in stages IV and V of the seminiferous epithelial cycle corresponding to meiotic divisions of primary spermatocytes and mitotic proliferation of B1 and B2 spermatogonia. Round and elongated spermatids were labeled less frequently by the TUNEL assay. To examine the relationships between germ cell apoptotic rate and spermatogenic efficiency, seminal traits were assessed to classify stallions into normal or reduced quality semen groups. Apoptotic rates were higher for stages IV-VI and stage VIII seminiferous tubules in stallions with reduced semen quality. Daily sperm production (DSP) per gram and per testis were lower for stallions with reduced semen quality. Regression analyses revealed negative linear relationships for germ cell apoptotic rate with DSP/g, DSP/testis, daily sperm output, progressively motile sperm and morphologically normal sperm in ejaculates. Mean circulating concentrations of inhibin were lower for stallions ejaculating reduced quality semen. Apoptotic rate was negatively correlated with concentrations of inhibin and estradiol-17b and positively correlated with concentrations of LH and FSH. To study germ cell apoptosis and formation of the Sertoli cell barrier during the initiation of spermatogenesis, tubule development was classified based on lumen score. Formation of a seminiferous tubule lumen was consistent with events leading to development of a Sertoli cell barrier. A primary wave of apoptosis removed early differentiating germ cell types prior to the formation of a tubule lumen facilitating both the formation of a tubule lumen and a Sertoli cell barrier. A second wave of apoptosis occurred after the formation of a lumen but before seminiferous tubule cross-sections contained a full complement of germ cells. In conclusion, apoptosis is an essential mechanism during normal spermatogenesis. Apoptosis also accounts for low numbers of normal sperm seen in the ejaculates of some stallions. Advisors/Committee Members: Forrest, David W. (advisor), Varner, Dickson D. (advisor), Blanchard, Terry L. (committee member), Johnson, Larry (committee member).

Subjects/Keywords: Apoptosis; spermatogenesis; stallion

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Heninger, Noah Leland, I. (2007). The role of testicular germ cell apoptosis during equine spermatogenesis. (Doctoral Dissertation). Texas A&M University. Retrieved from http://hdl.handle.net/1969.1/4691

Chicago Manual of Style (16^th Edition):

Heninger, Noah Leland, III. “The role of testicular germ cell apoptosis during equine spermatogenesis.” 2007. Doctoral Dissertation, Texas A&M University. Accessed March 07, 2021. http://hdl.handle.net/1969.1/4691.

MLA Handbook (7^th Edition):

Heninger, Noah Leland, III. “The role of testicular germ cell apoptosis during equine spermatogenesis.” 2007. Web. 07 Mar 2021.

Vancouver:

Heninger, Noah Leland I. The role of testicular germ cell apoptosis during equine spermatogenesis. [Internet] [Doctoral dissertation]. Texas A&M University; 2007. [cited 2021 Mar 07]. Available from: http://hdl.handle.net/1969.1/4691.

Council of Science Editors:

Heninger, Noah Leland I. The role of testicular germ cell apoptosis during equine spermatogenesis. [Doctoral Dissertation]. Texas A&M University; 2007. Available from: http://hdl.handle.net/1969.1/4691

University of Newcastle

23. McIver, Skye Courtney. Regulation of the transition from gonocytes to spermatogonia.

Degree: PhD, 2013, University of Newcastle

URL: http://hdl.handle.net/1959.13/1036706

►

Research Doctorate - Doctor of Philosophy (PhD)

In the western world there has been a significant decline in male reproductive health over the last 100… (more)

▼

Research Doctorate - Doctor of Philosophy (PhD)

In the western world there has been a significant decline in male reproductive health over the last 100 years, e.g. decreased sperm production and function and increased male infertility. Along with these more subtle changes the incidence of defects of reproductive development, including undescended testis and testicular germ cell cancer (seminoma and non-seminoma), have increased. Seminoma and non-seminoma are now the most common malignancy observed in young men. Both seminoma and non-seminoma develop from the pre-invasive lesion Carcinoma in Situ (CIS). The origin of CIS cells is still under debate however CIS cells are believed to result from improperly differentiated primordial germ cells (PGCs) or gonocytes. In general CIS cells express remarkably similar transcriptomes to gonocytes. Therefore it is essential to understand the cellular transition between gonocytes to spermatogonia to gain a better understanding of the aetiology of testicular germ cell tumours. MicroRNA (miRNA) are important regulators of posttranscriptional gene expression and have been identified as essential for embryogenesis, primordial germ cell differentiation and spermatogenesis. The aberrant expression of miRNA molecules is associated with male infertility and tumourigenesis including that of testicular germ cell tumours. We therefore examined the change in miRNA expression between postnatal gonocytes and spermatogonia for insights into how miRNA expression could influence this essential developmental process. We identified seven differentially expressed miRNA molecules between gonocytes and spermatogonia. The down regulated miRNA (miR-290.5p, 291a-5p, 293 and 294*) all belong to the miR-290 family, which is a key regulator of pluripotency as well as primordial germ cell survival and proliferation. The up-regulated miRNA (miR-136, 463* and 743a) are not as well known however miR-136 is thought to be a tumour suppressor and miR-743a is involved in oxidative stress. Target prediction software identified an abundance of targets in both the PTEN and Wnt signalling pathways. Wnt signalling is known to help maintain the undifferentiated spermatogonial stem cell population promote their proliferation. The ablation of PTEN expression is associated with the transformation of CIS into overt testicular germ cell cancer. The most highly expressed miRNAs, which were differentially expressed between gonocytes and spermatogonia, were selected for target identification using RNA/RNA pull-down as well as knockdown technology. The array analysis of the pull-down assay identified the presence of multiple targets from each of the miRNAs assayed (miR291a-5p, 293 and 743a) in the PDGF and RAC signalling pathways. PDGF has been documented to be involved in both the migration and proliferation of gonocytes. RAC signalling is required to transverse the blood testis barrier, which develops at postnatal day 8, and therefore is essential for later stages of spermatogenesis. Knockdown of miR291a-5p, 293 and 743a…

Advisors/Committee Members: University of Newcastle. Faculty of Science & Information Technology, School of Environmental and Life Sciences.

Subjects/Keywords: spermatogenesis; gonocytes; miRNA; differentiation; thesis by publication

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

McIver, S. C. (2013). Regulation of the transition from gonocytes to spermatogonia. (Doctoral Dissertation). University of Newcastle. Retrieved from http://hdl.handle.net/1959.13/1036706

Chicago Manual of Style (16^th Edition):

McIver, Skye Courtney. “Regulation of the transition from gonocytes to spermatogonia.” 2013. Doctoral Dissertation, University of Newcastle. Accessed March 07, 2021. http://hdl.handle.net/1959.13/1036706.

MLA Handbook (7^th Edition):

McIver, Skye Courtney. “Regulation of the transition from gonocytes to spermatogonia.” 2013. Web. 07 Mar 2021.

Vancouver:

McIver SC. Regulation of the transition from gonocytes to spermatogonia. [Internet] [Doctoral dissertation]. University of Newcastle; 2013. [cited 2021 Mar 07]. Available from: http://hdl.handle.net/1959.13/1036706.

Council of Science Editors:

McIver SC. Regulation of the transition from gonocytes to spermatogonia. [Doctoral Dissertation]. University of Newcastle; 2013. Available from: http://hdl.handle.net/1959.13/1036706

University of Texas Southwestern Medical Center

24. Goertz, Meredith Johanna. Foxo Transcription Factors Control Spermatogonial Stem Cell Self-Renewal and the Initiation of Spermatogenesis.

Degree: 2013, University of Texas Southwestern Medical Center

URL: http://hdl.handle.net/2152.5/1247

► Spermatogonial stem cells (SSCs), as the foundation for spermatogenesis, must maintain a balance of both self-renewal and differentiation. Although several factors important for these processes… (more)

Subjects/Keywords: Forkhead Transcription Factors; Spermatogenesis; Stem Cells

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Goertz, M. J. (2013). Foxo Transcription Factors Control Spermatogonial Stem Cell Self-Renewal and the Initiation of Spermatogenesis. (Thesis). University of Texas Southwestern Medical Center. Retrieved from http://hdl.handle.net/2152.5/1247

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Goertz, Meredith Johanna. “Foxo Transcription Factors Control Spermatogonial Stem Cell Self-Renewal and the Initiation of Spermatogenesis.” 2013. Thesis, University of Texas Southwestern Medical Center. Accessed March 07, 2021. http://hdl.handle.net/2152.5/1247.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Goertz, Meredith Johanna. “Foxo Transcription Factors Control Spermatogonial Stem Cell Self-Renewal and the Initiation of Spermatogenesis.” 2013. Web. 07 Mar 2021.

Vancouver:

Goertz MJ. Foxo Transcription Factors Control Spermatogonial Stem Cell Self-Renewal and the Initiation of Spermatogenesis. [Internet] [Thesis]. University of Texas Southwestern Medical Center; 2013. [cited 2021 Mar 07]. Available from: http://hdl.handle.net/2152.5/1247.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Goertz MJ. Foxo Transcription Factors Control Spermatogonial Stem Cell Self-Renewal and the Initiation of Spermatogenesis. [Thesis]. University of Texas Southwestern Medical Center; 2013. Available from: http://hdl.handle.net/2152.5/1247

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Texas Southwestern Medical Center

25. Baker, Michael David. The Small GTPase Rheb Is Required for Spermatogenesis but not Oogenesis.

Degree: 2013, University of Texas Southwestern Medical Center

URL: http://hdl.handle.net/2152.5/2712

► The process of germ cell development is under the tight control of various signaling pathways among which the PI3K-PKB-mTOR pathway is of critical importance. Previous… (more)

Subjects/Keywords: Monomeric GTP-Binding Proteins; Neuropeptides; Oogenesis; Spermatogenesis

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Baker, M. D. (2013). The Small GTPase Rheb Is Required for Spermatogenesis but not Oogenesis. (Thesis). University of Texas Southwestern Medical Center. Retrieved from http://hdl.handle.net/2152.5/2712

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Baker, Michael David. “The Small GTPase Rheb Is Required for Spermatogenesis but not Oogenesis.” 2013. Thesis, University of Texas Southwestern Medical Center. Accessed March 07, 2021. http://hdl.handle.net/2152.5/2712.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Baker, Michael David. “The Small GTPase Rheb Is Required for Spermatogenesis but not Oogenesis.” 2013. Web. 07 Mar 2021.

Vancouver:

Baker MD. The Small GTPase Rheb Is Required for Spermatogenesis but not Oogenesis. [Internet] [Thesis]. University of Texas Southwestern Medical Center; 2013. [cited 2021 Mar 07]. Available from: http://hdl.handle.net/2152.5/2712.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Baker MD. The Small GTPase Rheb Is Required for Spermatogenesis but not Oogenesis. [Thesis]. University of Texas Southwestern Medical Center; 2013. Available from: http://hdl.handle.net/2152.5/2712

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

East Carolina University

26. Kirsanov, Oleksandr. ANALYSIS OF TRANSGENIC MOUSE MODELS TO STUDY MAMMALIAN SPERMATOGONIA.

Degree: MS, MS-Biomedical Science, 2018, East Carolina University

URL: http://hdl.handle.net/10342/6969

► Spermatogenesis in the mammalian testis results in the daily production of millions of spermatozoa. This developmental process is founded upon the actions of a small… (more)

Subjects/Keywords: Spermatogonia; Animals; Mice, Transgenic; Mammals; Spermatogenesis

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Kirsanov, O. (2018). ANALYSIS OF TRANSGENIC MOUSE MODELS TO STUDY MAMMALIAN SPERMATOGONIA. (Masters Thesis). East Carolina University. Retrieved from http://hdl.handle.net/10342/6969

Chicago Manual of Style (16^th Edition):

Kirsanov, Oleksandr. “ANALYSIS OF TRANSGENIC MOUSE MODELS TO STUDY MAMMALIAN SPERMATOGONIA.” 2018. Masters Thesis, East Carolina University. Accessed March 07, 2021. http://hdl.handle.net/10342/6969.

MLA Handbook (7^th Edition):

Kirsanov, Oleksandr. “ANALYSIS OF TRANSGENIC MOUSE MODELS TO STUDY MAMMALIAN SPERMATOGONIA.” 2018. Web. 07 Mar 2021.

Vancouver:

Kirsanov O. ANALYSIS OF TRANSGENIC MOUSE MODELS TO STUDY MAMMALIAN SPERMATOGONIA. [Internet] [Masters thesis]. East Carolina University; 2018. [cited 2021 Mar 07]. Available from: http://hdl.handle.net/10342/6969.

Council of Science Editors:

Kirsanov O. ANALYSIS OF TRANSGENIC MOUSE MODELS TO STUDY MAMMALIAN SPERMATOGONIA. [Masters Thesis]. East Carolina University; 2018. Available from: http://hdl.handle.net/10342/6969

University of Newcastle upon Tyne

27. Floros, Vasileios. An investigation of the role of Notch signalling in germ cell development using mouse embryonic stem cells.

Degree: PhD, 2013, University of Newcastle upon Tyne

URL: http://theses.ncl.ac.uk/jspui/handle/10443/2282 ; https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.603414

Subjects/Keywords: 571.8; Proteins, Spermatogenesis

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Floros, V. (2013). An investigation of the role of Notch signalling in germ cell development using mouse embryonic stem cells. (Doctoral Dissertation). University of Newcastle upon Tyne. Retrieved from http://theses.ncl.ac.uk/jspui/handle/10443/2282 ; https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.603414

Chicago Manual of Style (16^th Edition):

Floros, Vasileios. “An investigation of the role of Notch signalling in germ cell development using mouse embryonic stem cells.” 2013. Doctoral Dissertation, University of Newcastle upon Tyne. Accessed March 07, 2021. http://theses.ncl.ac.uk/jspui/handle/10443/2282 ; https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.603414.

MLA Handbook (7^th Edition):

Floros, Vasileios. “An investigation of the role of Notch signalling in germ cell development using mouse embryonic stem cells.” 2013. Web. 07 Mar 2021.

Vancouver:

Floros V. An investigation of the role of Notch signalling in germ cell development using mouse embryonic stem cells. [Internet] [Doctoral dissertation]. University of Newcastle upon Tyne; 2013. [cited 2021 Mar 07]. Available from: http://theses.ncl.ac.uk/jspui/handle/10443/2282 ; https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.603414.

Council of Science Editors:

Floros V. An investigation of the role of Notch signalling in germ cell development using mouse embryonic stem cells. [Doctoral Dissertation]. University of Newcastle upon Tyne; 2013. Available from: http://theses.ncl.ac.uk/jspui/handle/10443/2282 ; https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.603414

East Carolina University

28. Velte, Ellen Kay. The Role of Differential Sensitivity to Retinoic Acid (RA) in Regulating Spermatogonial Fate.

Degree: PhD, PHD-Anatomy and Cell Biology, 2018, East Carolina University

URL: http://hdl.handle.net/10342/7078

► In the mammalian testis, the foundation of spermatogenesis is provided by spermatogonial stem cells (SSCs); their progeny either remain as stem cells (following a self-renewal… (more)

Subjects/Keywords: retinoic acid; CYP26; talarozole; Spermatogonia; Tretinoin; Spermatogenesis

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Velte, E. K. (2018). The Role of Differential Sensitivity to Retinoic Acid (RA) in Regulating Spermatogonial Fate. (Doctoral Dissertation). East Carolina University. Retrieved from http://hdl.handle.net/10342/7078

Chicago Manual of Style (16^th Edition):

Velte, Ellen Kay. “The Role of Differential Sensitivity to Retinoic Acid (RA) in Regulating Spermatogonial Fate.” 2018. Doctoral Dissertation, East Carolina University. Accessed March 07, 2021. http://hdl.handle.net/10342/7078.

MLA Handbook (7^th Edition):

Velte, Ellen Kay. “The Role of Differential Sensitivity to Retinoic Acid (RA) in Regulating Spermatogonial Fate.” 2018. Web. 07 Mar 2021.

Vancouver:

Velte EK. The Role of Differential Sensitivity to Retinoic Acid (RA) in Regulating Spermatogonial Fate. [Internet] [Doctoral dissertation]. East Carolina University; 2018. [cited 2021 Mar 07]. Available from: http://hdl.handle.net/10342/7078.

Council of Science Editors:

Velte EK. The Role of Differential Sensitivity to Retinoic Acid (RA) in Regulating Spermatogonial Fate. [Doctoral Dissertation]. East Carolina University; 2018. Available from: http://hdl.handle.net/10342/7078

University of Illinois – Urbana-Champaign

29. Bi, Jiajia. Expression and function of basigin during early pregnancy and spermatogenesis.

Degree: PhD, 0002, 2013, University of Illinois – Urbana-Champaign

URL: http://hdl.handle.net/2142/44411

► Basigin (BSG) is a multifunctional glycoprotein that plays an important role in both female and male reproduction since female knockout (KO) mice are infertile and… (more)

▼ Basigin (BSG) is a multifunctional glycoprotein that plays an important role in both female and male reproduction since female knockout (KO) mice are infertile and male KO mice are sterile. The aim of the present study was to determine 1) whether BSG is required for proliferation of the uterine luminal epithelium during early pregnancy in preparation for implantation; 2) whether BSG is required for HESCs decidualization; and 3) whether BSG is essential for the interactions between gametes and Sertoli cells during spermatogenesis. BSG protein was expressed in the uterine epithelium at estrus in βERKO mice but not in αERKO mice. However, a higher level of Bsg mRNA was observed in the uteri of αERKO mice as compared with wild type (WT) and βERKO mice. In the mouse, estrogen alone induces the proliferation of both luminal and glandular epithelial cells during early pregnancy. On day 1 of pregnancy, the expression levels of ERα and a well-known estrogen responsive gene, MUC1, appeared to be normal in the uteri of Bsg KO females. This suggested that the circulating estradiol levels in Bsg KO mice are normal. I examined proliferation in uterine epithelial cells and found that, in WT mice, uterine epithelial cells were highly proliferative as measured by expression of Ki67 in both the luminal and glandular epithelial cells. In contrast, Ki67 expression was significantly decreased in the epithelial cells of Bsg KO uteri. Immunochemistry using the BSG antibody revealed that the expression of BSG in C/EBPβ null uteri was heterogeneous and spotty in the luminal epithelium as compared with a more homogeneous expression pattern of BSG in the uterine epithelium of WT uteri. These results indicate that C/EBPβ may be one of the factors regulating the expression of BSG in mouse uterine epithelium. Decidualization of human endometrial stromal cells (HESCs) into decidual cells is carefully controlled, both spatially and temporally, by a balance of stimulatory and inhibitory signals from the uterine endometrium and is a prerequisite for successful implantation. Decidualization is associated with induction of a number of genes including wingless-type MMTV integration site family member (WNT4) and forkhead transcription factor forkhead box O1A (FOXO1). In the present study, HESCs were transfected with small interfering RNAs targeting BSG gene expression. Expression of the decidualization markers Insulin-like growth factor-binding protein 1 (IGFBP1) and Prolactin (PRL) was significantly inhibited in cells with down regulated BSG expression. Silencing of BSG in HESCs also impaired expression of several MMPs. Microarray analysis revealed that both WNT4 and FOXO1 and its downstream targets are under the regulation of BSG during decidualization in HESCs. The Bsg KO testis lacks elongated spermatids and mature spermatozoa, a phenotype similar to that of alpha-mannosidase IIx (MX) KO mice. MX regulates formation of N-acetylglucosamine (GlcNAc) terminated N-glycans that participate in germ cell-Sertoli cell adhesion. Results showed that Bsg… Advisors/Committee Members: Nowak, Romana A. (advisor), Nowak, Romana A. (Committee Chair), Bahr, Janice M. (committee member), Miller, David J. (committee member), Li, Quanxi (committee member).

Subjects/Keywords: Basigin (BSG); Implantation; decidualization; spermatogenesis; Infertility; Sterility

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Bi, J. (2013). Expression and function of basigin during early pregnancy and spermatogenesis. (Doctoral Dissertation). University of Illinois – Urbana-Champaign. Retrieved from http://hdl.handle.net/2142/44411

Chicago Manual of Style (16^th Edition):

Bi, Jiajia. “Expression and function of basigin during early pregnancy and spermatogenesis.” 2013. Doctoral Dissertation, University of Illinois – Urbana-Champaign. Accessed March 07, 2021. http://hdl.handle.net/2142/44411.

MLA Handbook (7^th Edition):

Bi, Jiajia. “Expression and function of basigin during early pregnancy and spermatogenesis.” 2013. Web. 07 Mar 2021.

Vancouver:

Bi J. Expression and function of basigin during early pregnancy and spermatogenesis. [Internet] [Doctoral dissertation]. University of Illinois – Urbana-Champaign; 2013. [cited 2021 Mar 07]. Available from: http://hdl.handle.net/2142/44411.

Council of Science Editors:

Bi J. Expression and function of basigin during early pregnancy and spermatogenesis. [Doctoral Dissertation]. University of Illinois – Urbana-Champaign; 2013. Available from: http://hdl.handle.net/2142/44411

University of Minnesota

30. Zhang, Teng. Investigation of the doublesex and mab-3 related transcription factors (Dmrts) in mammalian germ cell development.

Degree: PhD, Molecular, Cellular, Developmental Biology and Genetics, 2016, University of Minnesota

URL: http://hdl.handle.net/11299/181692

► My thesis work is focused on mammalian germ cell development, specifically focusing on the Dmrt (Doublesex and mab-3 related transcription factor) genes, which play key… (more)

▼ My thesis work is focused on mammalian germ cell development, specifically focusing on the Dmrt (Doublesex and mab-3 related transcription factor) genes, which play key roles in gonad and germ cell development in a broad range of species. Male mammals produce prodigious quantities of sperm continuously over a long reproductive life. What makes this possible is a population of spermatogonial stem cells (SSCs), which support a complex differentiation program that includes a series of proliferative spermatogonial divisions followed by meiosis and spermiation. My work has focused on how spermatogonial development is coordinated and controlled to sustain fertility. For my thesis research, I have aimed to understand how two related transcription factors, DMRT1 and DMRT6, coordinate mammalian spermatogenesis, from spermatogonial stem cell (SSC) development to meiotic entry. DMRT6 is expressed only in differentiating spermatogonia and is a direct target of DMRT1. Therefore, I hypothesized that DMRT6 functions downstream of DMRT1 in spermatogenesis to regulate the final steps leading to meiotic entry. I generated a conditional Dmrt6 allele and found that mutants on the C57BL/6J strain have severe defects in spermatogonial development leading to infertility. This phenotype was caused by inappropriate expression of spermatogonial differentiation factors and resulted in most late spermatogonia undergoing apoptosis. On the 129Sv background, mutant germ cells could complete spermatogonial differentiation and enter meiosis, but they showed defects in meiotic chromosome pairing, establishment of the XY body, and processing of recombination foci, and they mainly arrested in mid-pachynema. I also performed mRNA profiling of Dmrt6 mutant testes together with DMRT6 ChIP-seq to show that DMRT6 represses genes involved in spermatogonial differentiation and activates genes required for meiotic prophase. My results indicated that DMRT6 promotes meiotic entry by shutting down the spermatogonial differentiation program, and at the same time activates key meiotic genes to coordinate the transition from mitosis to meiosis. This finding was significant because previous studies only identified genes that function in either mitotic or meiotic development but I identified a gene that bridges this gap to coordinate the transition between these two developmental programs. . DMRT1 is expressed in spermatogonia, and its expression is silenced when cells enter meiosis. Previous work from our lab showed that DMRT1 controls the mitosis/meiosis switch. I focused on its roles early in spermatogonial development. Because DMRT1 is expressed both in SSCs and committed progenitor cells (spermatogonia), I hypothesized that DMRT1 is also required for SSC development. My work with Dmrt1 in mice has revealed that Dmrt1 is required to maintain SSCs during steady state spermatogenesis, where it appears to transcriptionally activate critical regulators of SSC maintenance including Plzf. I also found that Dmrt1 is required for regeneration of SSCs after…

Subjects/Keywords: DMRT; germ cells; meiosis; spermatogenesis; Testis

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Zhang, T. (2016). Investigation of the doublesex and mab-3 related transcription factors (Dmrts) in mammalian germ cell development. (Doctoral Dissertation). University of Minnesota. Retrieved from http://hdl.handle.net/11299/181692

Chicago Manual of Style (16^th Edition):

Zhang, Teng. “Investigation of the doublesex and mab-3 related transcription factors (Dmrts) in mammalian germ cell development.” 2016. Doctoral Dissertation, University of Minnesota. Accessed March 07, 2021. http://hdl.handle.net/11299/181692.

MLA Handbook (7^th Edition):

Zhang, Teng. “Investigation of the doublesex and mab-3 related transcription factors (Dmrts) in mammalian germ cell development.” 2016. Web. 07 Mar 2021.

Vancouver:

Zhang T. Investigation of the doublesex and mab-3 related transcription factors (Dmrts) in mammalian germ cell development. [Internet] [Doctoral dissertation]. University of Minnesota; 2016. [cited 2021 Mar 07]. Available from: http://hdl.handle.net/11299/181692.

Council of Science Editors:

Zhang T. Investigation of the doublesex and mab-3 related transcription factors (Dmrts) in mammalian germ cell development. [Doctoral Dissertation]. University of Minnesota; 2016. Available from: http://hdl.handle.net/11299/181692

Open Access Theses and Dissertations