subject:(spermatogenesis)

University of Hong Kong

1. Yeung, Seen-yu, Aurora. Regulation and functional studies of the testis-specific temperature-related sequence 4 (TRS4) in spermatogenesis.

Degree: M. Phil., 2014, University of Hong Kong

URL: Yeung, S. A. [楊善如]. (2014). Regulation and functional studies of the testis-specific temperature-related sequence 4 (TRS4) in spermatogenesis. (Thesis). University of Hong Kong, Pokfulam, Hong Kong SAR. Retrieved from http://dx.doi.org/10.5353/th_b5351046 ; http://dx.doi.org/10.5353/th_b5351046 ; http://hdl.handle.net/10722/208024

► Temperature-related sequence 4 (Trs4) is a transcript identified from the testis of a cryptorchid rat model. TRS4 is firstly detected in round spermatids on postnatal… (more)

▼ Temperature-related sequence 4 (Trs4) is a transcript identified from the testis of a cryptorchid rat model. TRS4 is firstly detected in round spermatids on postnatal day 21 mice, and is localized on the acrosome and tails of elongating spermatids and mature spermatozoa, suggesting a role in spermatogenesis. The present study aimed to investigate the regulatory and functional roles of Trs4 in spermatogenesis. To investigate the hormonal regulation of Trs4 in vitro, mouse germ cell were co-cultured with Sertoli (TM4) cell-line for 24 hours with 1μM or 10nM testosterone and the expression of Trs4 was quantitated. It was found that testosterone treatment at physiological level (10nM) significantly down-regulated Trs4 mRNA expression. However, TRS4 protein level did not change significantly. To investigate the functional roles of Trs4 and its interacting proteins (Rshl2, Gstmu1 and Ddc8) in spermatogenesis, the scrotal heat-treated rat model was used. The expression of Trs4, Rshl2, Gstmu1 and Ddc8 transcript were investigated on day 1, 9, 25, 37, 46, 56 and 79 post-heat treatment. Trs4, Rshl2 and Ddc8 transcripts were down-regulated on day 9 after heat-treatment, and recovered from day 37 onward. Immunohistochemical staining of TRS4 and RSHL-2 proteins showed they specifically localized in the cytoplasm of elongating spermatids in rat seminiferous tubules, suggesting that they might interact in regulating the development of elongating spermatids. Ten sessions of electroacupuncture (EA) was applied to the rats on day 9-36 to study the effect of EA in spermatogenesis. It was found that EA did not have any effect on the expression of Trs4, Rshl-2 and Ddc8 during recovery from hyperthermia when compared to the heat-treated only group. However, qPCR analysis on Gstmu1 mRNA expression showed a significant decrease in expression on day 46, and consistent with our TUNEL assay indicating a decrease in apoptosis in the EA group. Therefore, it is possible that Trs4 interacts with Gstmu1 and acts as anti-apoptotic factor for cell survival. To understand functional roles of Trs4 in mouse spermatogenesis, five Trs4 chimeric mice were generated in our laboratory and germline transmissible 〖Trs4〗^(flox/+) mice were identified. Sycp1-Cre mice were used to breed with Trs4 floxed mice to generate Trs4 floxed mice expressing Cre recombinase in spermatocytes (〖Trs4〗^(flox/+);〖Sycp-Cre〗^(+/0)) and aiming to generate Trs4 heterozygous KO mouse. However, after screening 74 offspring, no Trs4 heterozygous KO mouse was identified in this study and was likely to be caused by the low efficiency of recombination in Sycp1-Cre mice. Then, Zp3-Cre mice that expressing Cre-recombinase in oocyte were employed. Crossing of 〖Trs4〗^(flox/+):Zp3-〖Cre〗^(+/0) mice and Trs4 floxed mice could not produce heterozygous or homozygous Trs4 KO mouse, but suggested an aberrant recombination process happened in vivo. PCR assay confirmed DNA rearrangement occurred in regions flanking the exon 4 to the second LoxP site in Trs4 floxed mice. Furthermore, the…

Subjects/Keywords: Spermatogenesis

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Yeung, Seen-yu, A. (2014). Regulation and functional studies of the testis-specific temperature-related sequence 4 (TRS4) in spermatogenesis. (Masters Thesis). University of Hong Kong. Retrieved from Yeung, S. A. [楊善如]. (2014). Regulation and functional studies of the testis-specific temperature-related sequence 4 (TRS4) in spermatogenesis. (Thesis). University of Hong Kong, Pokfulam, Hong Kong SAR. Retrieved from http://dx.doi.org/10.5353/th_b5351046 ; http://dx.doi.org/10.5353/th_b5351046 ; http://hdl.handle.net/10722/208024

Chicago Manual of Style (16^th Edition):

Yeung, Seen-yu, Aurora. “Regulation and functional studies of the testis-specific temperature-related sequence 4 (TRS4) in spermatogenesis.” 2014. Masters Thesis, University of Hong Kong. Accessed December 15, 2019. Yeung, S. A. [楊善如]. (2014). Regulation and functional studies of the testis-specific temperature-related sequence 4 (TRS4) in spermatogenesis. (Thesis). University of Hong Kong, Pokfulam, Hong Kong SAR. Retrieved from http://dx.doi.org/10.5353/th_b5351046 ; http://dx.doi.org/10.5353/th_b5351046 ; http://hdl.handle.net/10722/208024.

MLA Handbook (7^th Edition):

Yeung, Seen-yu, Aurora. “Regulation and functional studies of the testis-specific temperature-related sequence 4 (TRS4) in spermatogenesis.” 2014. Web. 15 Dec 2019.

Vancouver:

Yeung, Seen-yu A. Regulation and functional studies of the testis-specific temperature-related sequence 4 (TRS4) in spermatogenesis. [Internet] [Masters thesis]. University of Hong Kong; 2014. [cited 2019 Dec 15]. Available from: Yeung, S. A. [楊善如]. (2014). Regulation and functional studies of the testis-specific temperature-related sequence 4 (TRS4) in spermatogenesis. (Thesis). University of Hong Kong, Pokfulam, Hong Kong SAR. Retrieved from http://dx.doi.org/10.5353/th_b5351046 ; http://dx.doi.org/10.5353/th_b5351046 ; http://hdl.handle.net/10722/208024.

Council of Science Editors:

Yeung, Seen-yu A. Regulation and functional studies of the testis-specific temperature-related sequence 4 (TRS4) in spermatogenesis. [Masters Thesis]. University of Hong Kong; 2014. Available from: Yeung, S. A. [楊善如]. (2014). Regulation and functional studies of the testis-specific temperature-related sequence 4 (TRS4) in spermatogenesis. (Thesis). University of Hong Kong, Pokfulam, Hong Kong SAR. Retrieved from http://dx.doi.org/10.5353/th_b5351046 ; http://dx.doi.org/10.5353/th_b5351046 ; http://hdl.handle.net/10722/208024

Oregon State University

2. Longo, Frank J., 1939-. The fine structure of spermatid differentiation in the mussel, Mytilus edulis, Linn.

Degree: PhD, Zoology, 1967, Oregon State University

URL: http://hdl.handle.net/1957/47217

► The spermiogenesis of Mytilus edulis (a marine mussel) has been studied, employing techniques of light and electron microscopy and cytochemical preparations. The observations trace the… (more)

▼ The spermiogenesis of Mytilus edulis (a marine mussel) has been studied, employing techniques of light and electron microscopy and cytochemical preparations. The observations trace the development of the acrosomal complex, the nucleus, the mitochondrial middle-piece, and the flagellum. In early spermatids numerous membrane-bounded proacrosomal vesicles arise from several Golgi complexes and coalesce to form a single large acrosomal vesicle. This comes to rest on the anterior surface of the nucleus and invaginates to become an elongated cone-shaped structure. An electron dense, spherical mass of membranoid sleeve material, also arising from the Golgi complex, adjoins the acrosomal vesicle and later occupies its indentation. In mature spermatozoa the membranoid sleeve lines the cavity of the cone-shaped acrosomal vesicle and surrounds the axial rod. Precursor material of the axial rod is first observed between the nucleus and the acrosomal vesicle as an aggregation of fibers. This later extends posteriorly into the nucleus and anteriorly into the cavity of the acrosomal vesicle. In the mature spermatozoon the fibrous axial rod ends posteriorly in the centriolar region of the middle-piece. The endoplasmic reticulum of early spermatids is a rudimentary system of membrane-bounded canaliculi and flattened vesicles which is predominantly free of ribosomes. Independent or clustered ribosome-like particles are present in the ground cytoplasm. By late spermiogenesis the reticulum has largely disappeared and the ground cytoplasm containing a dense particulate aggregate is sloughed off. In the early spermatid a dense filamentous network of chromatin in the interior and along the periphery of the nucleus increases in size by accretion of the finer karyoplasm. Eventually the interaggregate spaces are obliterated as the large, coarsely granular masses coalesce to form the compact, barrel-shaped nucleus of the sperm. By mid-spermiogenesis the randomly distributed elipsoid mitochondria become localized around the nucleus. The association with the nucleus is intimate and in many cases the mitochondria may be found in shallow recesses with the nuclear envelope reflected over their surface. Subsequently the mitochondria became spheroid, reduced to five in number, and restricted to the sperm middle-piece. Two cylindrical centrioles are situated near the base of the nucleus and are surrounded by the mitochondria of the middle-piece. The distal centriole elongates and from it the longitudinal fibrils of the sperm flagellum originate. The proximal centriole, oriented obliquely to the distal centriole, retains its initial structure. Tests with Sudan black B showed the presence of phospholipids in the mitochondria of late spermatids and mature sperm. Cytochemical techniques for carbohydrates indicated a neutral mucoprotein for the proacrosomal vesicles and allowed these to be identified in early spermatogenesis. The development of the Mytilus spermatid was compared with that of other vertebrate… Advisors/Committee Members: Dornfeld, E. J. (advisor).

Subjects/Keywords: Spermatogenesis

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APA (6^th Edition):

Longo, Frank J., 1. (1967). The fine structure of spermatid differentiation in the mussel, Mytilus edulis, Linn. (Doctoral Dissertation). Oregon State University. Retrieved from http://hdl.handle.net/1957/47217

Chicago Manual of Style (16^th Edition):

Longo, Frank J., 1939-. “The fine structure of spermatid differentiation in the mussel, Mytilus edulis, Linn.” 1967. Doctoral Dissertation, Oregon State University. Accessed December 15, 2019. http://hdl.handle.net/1957/47217.

MLA Handbook (7^th Edition):

Longo, Frank J., 1939-. “The fine structure of spermatid differentiation in the mussel, Mytilus edulis, Linn.” 1967. Web. 15 Dec 2019.

Vancouver:

Longo, Frank J. 1. The fine structure of spermatid differentiation in the mussel, Mytilus edulis, Linn. [Internet] [Doctoral dissertation]. Oregon State University; 1967. [cited 2019 Dec 15]. Available from: http://hdl.handle.net/1957/47217.

Council of Science Editors:

Longo, Frank J. 1. The fine structure of spermatid differentiation in the mussel, Mytilus edulis, Linn. [Doctoral Dissertation]. Oregon State University; 1967. Available from: http://hdl.handle.net/1957/47217

University of Hong Kong

3. Tang, Elizabeth Ingrid Yingyu. Regulation of the cytoskeleton during spermatogenesis.

Degree: PhD, 2016, University of Hong Kong

URL: http://hdl.handle.net/10722/238350

► Spermatogenesis is a complex process that takes place in the seminiferous tubules of the mammalian testes to produce spermatozoa. The epithelium of the seminiferous tubules… (more)

▼ Spermatogenesis is a complex process that takes place in the seminiferous tubules of the mammalian testes to produce spermatozoa. The epithelium of the seminiferous tubules is comprised of germ cells (GC), at different stages of development, and Sertoli cells (SC). The SC has the task of creating the blood-testis barrier (BTB), providing nutritional support to GCs, and supporting GC transport across the BTB and epithelium. As the SC is involved in such critical and dynamic processes, it is conceivable that SCs are tightly regulated in order to ensure the cellular events of spermatogenesis are carried out flawlessly. A dynamic cytoskeleton enables the SC to do this. Of the three major classes of filamentous proteins in the SC cytoskeleton: actin filaments, intermediate filaments, and microtubules (MT), actin filaments are the best-studied component in SCs. However, the cytoskeleton cannot be fully understood by looking at its individual components, because they all work together to form the cytoskeleton. Researchers across different fields have found that the relationship between actin filaments and MTs is greater than originally thought. The current understanding of SC MTs is that they are essential for structural support of the cell and providing tracks for GC transport across the epithelium, but, beyond this function, little is known about their regulation and interaction with other cytoskeleton components. Herein it is proposed that SC MTs are involved in regulating the ectoplasmic specialization (ES) and BTB, which are actin-rich cell junction sites within the seminiferous epithelium, as well as transport during spermatogenesis. The studies presented here aim to address the regulation of SC MTs during spermatogenesis. The functions of MT regulatory proteins: MAP/MT affinity-regulating kinases 2 and 4 (MARK2, MARK4) and end-binding protein 1 (EB1), were investigated. MARK2 and MARK4 were shown to be involved in maintenance of the ES and BTB by regulating MTs. Knockdown of MARK2 versus MARK4 in vitro by RNAi in cultured rat SCs revealed that the isoforms differentially regulated the SC ES and BTB. Loss of MARK2 function led to perturbation of TJ-permeability barrier via disruption of TJ protein distribution. Similarly, MARK4 knockdown perturbed the TJ-permeability barrier, however effects were more pronounced than in MARK2 RNAi cells, as its knockdown led to mislocalization of basal ES and TJ proteins. Knockdown of EB1 also perturbed the TJ-permeability barrier by causing mislocalization of junction proteins. Reduction of either MARK2, MARK4, or EB1 disrupted MT distribution throughout SCs, leading to mislocalization of junction proteins. Additionally, it was discovered that alterations in MT arrangement in SCs affected the actin cytoskeleton, which directly supports BTB function. Following these studies, the adjudin model was used to relate these findings to in vivo conditions. Adjudin is a potential male contraceptive and disrupts actin-based adhesion to cause premature GC/spermatid loss. Despite actin…

Subjects/Keywords: Cytoskeleton; Spermatogenesis

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Tang, E. I. Y. (2016). Regulation of the cytoskeleton during spermatogenesis. (Doctoral Dissertation). University of Hong Kong. Retrieved from http://hdl.handle.net/10722/238350

Chicago Manual of Style (16^th Edition):

Tang, Elizabeth Ingrid Yingyu. “Regulation of the cytoskeleton during spermatogenesis.” 2016. Doctoral Dissertation, University of Hong Kong. Accessed December 15, 2019. http://hdl.handle.net/10722/238350.

MLA Handbook (7^th Edition):

Tang, Elizabeth Ingrid Yingyu. “Regulation of the cytoskeleton during spermatogenesis.” 2016. Web. 15 Dec 2019.

Vancouver:

Tang EIY. Regulation of the cytoskeleton during spermatogenesis. [Internet] [Doctoral dissertation]. University of Hong Kong; 2016. [cited 2019 Dec 15]. Available from: http://hdl.handle.net/10722/238350.

Council of Science Editors:

Tang EIY. Regulation of the cytoskeleton during spermatogenesis. [Doctoral Dissertation]. University of Hong Kong; 2016. Available from: http://hdl.handle.net/10722/238350

Oregon State University

4. Lin, Samuel. A study of the effect of 1,2-alpha-methlene-6-chloro-delta-4, 6-17-alpha-hydroxy progesterone acetate on the adult male rat including changes in the cell associations of the germinal epithelium.

Degree: MS, Zoology, 1967, Oregon State University

URL: http://hdl.handle.net/1957/47175

► SH 714 (1, 2-alpha-methylene-6-chloro-delta-4, 6-17-alphahydroxyprogesterone acetate) synthesized by Schering AG is an highly antiandrogenic, nonestrogenic compound. The present investigation was undertaken to determine the compound's… (more)

Subjects/Keywords: Spermatogenesis

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Lin, S. (1967). A study of the effect of 1,2-alpha-methlene-6-chloro-delta-4, 6-17-alpha-hydroxy progesterone acetate on the adult male rat including changes in the cell associations of the germinal epithelium. (Masters Thesis). Oregon State University. Retrieved from http://hdl.handle.net/1957/47175

Chicago Manual of Style (16^th Edition):

Lin, Samuel. “A study of the effect of 1,2-alpha-methlene-6-chloro-delta-4, 6-17-alpha-hydroxy progesterone acetate on the adult male rat including changes in the cell associations of the germinal epithelium.” 1967. Masters Thesis, Oregon State University. Accessed December 15, 2019. http://hdl.handle.net/1957/47175.

MLA Handbook (7^th Edition):

Lin, Samuel. “A study of the effect of 1,2-alpha-methlene-6-chloro-delta-4, 6-17-alpha-hydroxy progesterone acetate on the adult male rat including changes in the cell associations of the germinal epithelium.” 1967. Web. 15 Dec 2019.

Vancouver:

Lin S. A study of the effect of 1,2-alpha-methlene-6-chloro-delta-4, 6-17-alpha-hydroxy progesterone acetate on the adult male rat including changes in the cell associations of the germinal epithelium. [Internet] [Masters thesis]. Oregon State University; 1967. [cited 2019 Dec 15]. Available from: http://hdl.handle.net/1957/47175.

Council of Science Editors:

Lin S. A study of the effect of 1,2-alpha-methlene-6-chloro-delta-4, 6-17-alpha-hydroxy progesterone acetate on the adult male rat including changes in the cell associations of the germinal epithelium. [Masters Thesis]. Oregon State University; 1967. Available from: http://hdl.handle.net/1957/47175

Michigan State University

5. Cattani, Carola Marietta. Preferential segregation of the Y chromosome in spermatogenesis of Drosophila melanogaster.

Degree: MS, Department of Zoology, 1966, Michigan State University

URL: http://etd.lib.msu.edu/islandora/object/etd:40457

Subjects/Keywords: Spermatogenesis

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Cattani, C. M. (1966). Preferential segregation of the Y chromosome in spermatogenesis of Drosophila melanogaster. (Masters Thesis). Michigan State University. Retrieved from http://etd.lib.msu.edu/islandora/object/etd:40457

Chicago Manual of Style (16^th Edition):

Cattani, Carola Marietta. “Preferential segregation of the Y chromosome in spermatogenesis of Drosophila melanogaster.” 1966. Masters Thesis, Michigan State University. Accessed December 15, 2019. http://etd.lib.msu.edu/islandora/object/etd:40457.

MLA Handbook (7^th Edition):

Cattani, Carola Marietta. “Preferential segregation of the Y chromosome in spermatogenesis of Drosophila melanogaster.” 1966. Web. 15 Dec 2019.

Vancouver:

Cattani CM. Preferential segregation of the Y chromosome in spermatogenesis of Drosophila melanogaster. [Internet] [Masters thesis]. Michigan State University; 1966. [cited 2019 Dec 15]. Available from: http://etd.lib.msu.edu/islandora/object/etd:40457.

Council of Science Editors:

Cattani CM. Preferential segregation of the Y chromosome in spermatogenesis of Drosophila melanogaster. [Masters Thesis]. Michigan State University; 1966. Available from: http://etd.lib.msu.edu/islandora/object/etd:40457

University of Hong Kong

6. Zhang, Xu. Regulation of testicular cell junction dynamics.

Degree: PhD, 2014, University of Hong Kong

URL: Zhang, X. [张栩]. (2014). Regulation of testicular cell junction dynamics. (Thesis). University of Hong Kong, Pokfulam, Hong Kong SAR. Retrieved from http://dx.doi.org/10.5353/th_b5312329 ; http://dx.doi.org/10.5353/th_b5312329 ; http://hdl.handle.net/10722/216333

► Spermatogenesis is an important cellular process in which mature haploid spermatozoa are produced from diploid spermatogonia and finally released from the seminiferous epithelium into the… (more)

▼ Spermatogenesis is an important cellular process in which mature haploid spermatozoa are produced from diploid spermatogonia and finally released from the seminiferous epithelium into the tubular lumen. This process involves extensive and timely restructuring of cell junctions at the Sertoli-Sertoli cell interface where the blood-testis barrier (BTB) and basal ectoplasmic specialization (ES) are located and at the Sertoli-germ cell interface where apical ES is present. The restructuring of cell junctions is involved in facilitating the translocation of spermatocytes across the BTB into adluminal compartment of the seminiferous epithelium as well as the detachment of mature elongated spermatids from the epithelium for release. Turnover of the BTB and Sertoli-mature spermatid contact (apical ES) can be interrupted by environmental toxicant such as cadmium (Cd) and is tightly regulated by cytokines such as transforming growth factor betas (TGF-βs). However, the molecular mechanisms involved in the regulation of testicular cell junction dynamics have not been completely explored. This dissertation focused on examining the mechanisms on how Cd and TGF-β3 regulate junction proteins, nectin-2 and JAM-B respectively. Nectin-2 is found at the basal and apical ES for the formation of the BTB and Sertoli-spermatid adhesion. Results of this study have demonstrated that CdCl2 reduces nectin-2 mRNA and protein levels and causes the loss of nectin-2 at the cell-cell interface. Inhibitor and shRNA knockdown have shown that CdCl2 speeds up nectin-2 protein degradation through clathrin-mediated endocytosis. Immunofluorescence staining and endocytosis assays further confirmed that nectin-2 internalization is promoted upon Cd treatment. In addition, Cd causes nectin-2 gene repression by reducing the bioavailability of endogenous transcription factors as well as impeding the binding of positive regulators to the promoter region. Collectively, these results have unraveled a novel and direct regulatory mechanism of Cd-mediated male reproductive dysfunction and explain why testis is more susceptible to Cd toxicity than other tissues. Junctional adhesion molecule-B (JAM-B) is found between Sertoli cells at the BTB as well as between Sertoli and germ cells at the apical ES in the testis. It was found that TGF-β3 downregulates JAM-B mRNA and protein levels, and reduces the bioavailability of JAM-B at the site of cell-cell contact. TGF-β3 promotes JAM-B protein degradation via ubiquitin-proteasome pathway with the activation of Smad signaling cascade. Besides, TGF-β3 destabilizes JAM-B mRNA transcript via ERK1/2 and p54 JNK activation. These results have unraveled the mechanisms by which TGF-β3 exerts negative regulatory effects on JAM-B expression that is crucial for the BTB integrity and Sertoli-germ cell interaction. Taken together, the findings reported herein demonstrate that testicular cell junction proteins are regulated by environmental toxicant and cytokine at the transcriptional, post-transcriptional and…

Subjects/Keywords: Cell interaction; Spermatogenesis

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Zhang, X. (2014). Regulation of testicular cell junction dynamics. (Doctoral Dissertation). University of Hong Kong. Retrieved from Zhang, X. [张栩]. (2014). Regulation of testicular cell junction dynamics. (Thesis). University of Hong Kong, Pokfulam, Hong Kong SAR. Retrieved from http://dx.doi.org/10.5353/th_b5312329 ; http://dx.doi.org/10.5353/th_b5312329 ; http://hdl.handle.net/10722/216333

Chicago Manual of Style (16^th Edition):

Zhang, Xu. “Regulation of testicular cell junction dynamics.” 2014. Doctoral Dissertation, University of Hong Kong. Accessed December 15, 2019. Zhang, X. [张栩]. (2014). Regulation of testicular cell junction dynamics. (Thesis). University of Hong Kong, Pokfulam, Hong Kong SAR. Retrieved from http://dx.doi.org/10.5353/th_b5312329 ; http://dx.doi.org/10.5353/th_b5312329 ; http://hdl.handle.net/10722/216333.

MLA Handbook (7^th Edition):

Zhang, Xu. “Regulation of testicular cell junction dynamics.” 2014. Web. 15 Dec 2019.

Vancouver:

Zhang X. Regulation of testicular cell junction dynamics. [Internet] [Doctoral dissertation]. University of Hong Kong; 2014. [cited 2019 Dec 15]. Available from: Zhang, X. [张栩]. (2014). Regulation of testicular cell junction dynamics. (Thesis). University of Hong Kong, Pokfulam, Hong Kong SAR. Retrieved from http://dx.doi.org/10.5353/th_b5312329 ; http://dx.doi.org/10.5353/th_b5312329 ; http://hdl.handle.net/10722/216333.

Council of Science Editors:

Zhang X. Regulation of testicular cell junction dynamics. [Doctoral Dissertation]. University of Hong Kong; 2014. Available from: Zhang, X. [张栩]. (2014). Regulation of testicular cell junction dynamics. (Thesis). University of Hong Kong, Pokfulam, Hong Kong SAR. Retrieved from http://dx.doi.org/10.5353/th_b5312329 ; http://dx.doi.org/10.5353/th_b5312329 ; http://hdl.handle.net/10722/216333

University of Nairobi

7. Oduma, J.A. Effects of Ripe Carica Papaya Seed Powder on Testicular Histology of Boars .

Degree: 2012, University of Nairobi

URL: http://erepository.uonbi.ac.ke:8080/xmlui/handle/123456789/10910

► The effect of oral administration of ripe Carica Papaya seed powder on testicular histology of Sus scrofa domestica boars has been addressed in the present… (more)

Subjects/Keywords: Pawpaw seeds; Spermatogenesis; Testicular histology

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APA (6^th Edition):

Oduma, J. A. (2012). Effects of Ripe Carica Papaya Seed Powder on Testicular Histology of Boars . (Thesis). University of Nairobi. Retrieved from http://erepository.uonbi.ac.ke:8080/xmlui/handle/123456789/10910

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Oduma, J A. “Effects of Ripe Carica Papaya Seed Powder on Testicular Histology of Boars .” 2012. Thesis, University of Nairobi. Accessed December 15, 2019. http://erepository.uonbi.ac.ke:8080/xmlui/handle/123456789/10910.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Oduma, J A. “Effects of Ripe Carica Papaya Seed Powder on Testicular Histology of Boars .” 2012. Web. 15 Dec 2019.

Vancouver:

Oduma JA. Effects of Ripe Carica Papaya Seed Powder on Testicular Histology of Boars . [Internet] [Thesis]. University of Nairobi; 2012. [cited 2019 Dec 15]. Available from: http://erepository.uonbi.ac.ke:8080/xmlui/handle/123456789/10910.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Oduma JA. Effects of Ripe Carica Papaya Seed Powder on Testicular Histology of Boars . [Thesis]. University of Nairobi; 2012. Available from: http://erepository.uonbi.ac.ke:8080/xmlui/handle/123456789/10910

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Hong Kong

8. So, Kam-hei. A study on the role of temperature repressed sequence 4 (Trs4) in spermatogenesis.

Degree: M. Phil., 2011, University of Hong Kong

URL: So, K. [蘇錦熙]. (2011). A study on the role of temperature repressed sequence 4 (Trs4) in spermatogenesis. (Thesis). University of Hong Kong, Pokfulam, Hong Kong SAR. Retrieved from http://dx.doi.org/10.5353/th_b4731070 ; http://dx.doi.org/10.5353/th_b4731070 ; http://hdl.handle.net/10722/174371

► Heat stress inhibits spermatogenesis partly by inducing apoptosis in the testicular germ cells. Using a cryptochid rat model, we identified a temperature-related ESTs 4 (TRS4)… (more)

▼ Heat stress inhibits spermatogenesis partly by inducing apoptosis in the testicular germ cells. Using a cryptochid rat model, we identified a temperature-related ESTs 4 (TRS4) transcript from rat testis. Trs4 mRNA is specifically expressed in the mouse and rat testis from postnatal day 21 and 28 days onwards, respectively. Trs4 protein is located mainly in the elongating spermatids and mature spermatozoa at the acrosome and tail regions. Using a yeat-2-hybrid screening, Trs4 was found to bind Gstmu1, Rslh-2 and Ddc8 proteins. To further characterize the functional role of Trs4 in spermatogenesis, and study how Trs4 interacts its binding proteins for cellular functions, we aimed (1) to screen putative ES cells with Trs4 floxed allele for knockout mice generation, (2) to generate Trs4 deletion constructs and study the cellular localization of Trs4 and its putative binding partners in transfected spermatocyte GC-2spd(s) cell line, (3) to study how heat-treatment regulates the expression of Trs4 and apoptotic molecules. The Trs4 conditional targeting vector was constructed by flanking exons 4-6 with two LoxP sites and electroporated into ES cells. After screening of 480 clones, positive ES cell clones were identified by Southern blotting using 5’- and 3’- probes. Three putative positive clones were identified carrying the floxed allele. Trs4 protein contains putative ubiquitin-like motif (a.a. 119-224), IQ-calmodulin binding motif (a.a. 334-362) and a overlapping bipartite nuclear localization signal (BNL) (a.a. 346-362). Transfection of EGFP fused Trs4 truncated protein demonstrated that the IQ-calmodulin binding motif and BNL signal was important for localization of Trs4 protein in the cytoplasmic/Golgi regions; while the N-terminal contains ubiquitin-like motif and the C-terminal regions direct the expression of the EGFP-fusion protein mainly to the nucleus. The full-length sequence of Trs4 binding partners: Gstmu1, Rshl-2 and Ddc8 were cloned into the pDsRedmonomer-C1 vector, giving red fluorescence protein in the transfected cells. They were colocalized with EGFP-Trs4 in the cytoplasm of the cells, confirming that Trs4 and its interacting protein is likely interact with each other in vivo. As Trs4 colocalize with Gstmu1, a modulator of mitochondrial-dependent pathway in apoptosis, it is suggested that Trs4 is an upstream regulator of apoptosis under heat treatment in germ cells. The functional roles of Trs4 protein Trs4 and apoptotic molecules. The Trs4 conditional targeting vector was constructed by flanking exons 4-6 with two LoxP sites and electroporated into ES cells. After screening of 480 clones, positive ES cell clones were identified by Southern blotting using 5’- and 3’- probes. Three putative positive clones were identified carrying the floxed allele. Trs4 protein contains putative ubiquitin-like motif (a.a. 119-224), IQ-calmodulin binding motif (a.a. 334-362) and a overlapping bipartite nuclear localization signal (BNL) (a.a. 346-362). Transfection of EGFP fused Trs4 truncated protein demonstrated that… Advisors/Committee Members: Lee, CKF, Chiu, CN.

Subjects/Keywords: Spermatogenesis in animals - Genetic aspects.

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APA (6^th Edition):

So, K. (2011). A study on the role of temperature repressed sequence 4 (Trs4) in spermatogenesis. (Masters Thesis). University of Hong Kong. Retrieved from So, K. [蘇錦熙]. (2011). A study on the role of temperature repressed sequence 4 (Trs4) in spermatogenesis. (Thesis). University of Hong Kong, Pokfulam, Hong Kong SAR. Retrieved from http://dx.doi.org/10.5353/th_b4731070 ; http://dx.doi.org/10.5353/th_b4731070 ; http://hdl.handle.net/10722/174371

Chicago Manual of Style (16^th Edition):

So, Kam-hei. “A study on the role of temperature repressed sequence 4 (Trs4) in spermatogenesis.” 2011. Masters Thesis, University of Hong Kong. Accessed December 15, 2019. So, K. [蘇錦熙]. (2011). A study on the role of temperature repressed sequence 4 (Trs4) in spermatogenesis. (Thesis). University of Hong Kong, Pokfulam, Hong Kong SAR. Retrieved from http://dx.doi.org/10.5353/th_b4731070 ; http://dx.doi.org/10.5353/th_b4731070 ; http://hdl.handle.net/10722/174371.

MLA Handbook (7^th Edition):

So, Kam-hei. “A study on the role of temperature repressed sequence 4 (Trs4) in spermatogenesis.” 2011. Web. 15 Dec 2019.

Vancouver:

So K. A study on the role of temperature repressed sequence 4 (Trs4) in spermatogenesis. [Internet] [Masters thesis]. University of Hong Kong; 2011. [cited 2019 Dec 15]. Available from: So, K. [蘇錦熙]. (2011). A study on the role of temperature repressed sequence 4 (Trs4) in spermatogenesis. (Thesis). University of Hong Kong, Pokfulam, Hong Kong SAR. Retrieved from http://dx.doi.org/10.5353/th_b4731070 ; http://dx.doi.org/10.5353/th_b4731070 ; http://hdl.handle.net/10722/174371.

Council of Science Editors:

So K. A study on the role of temperature repressed sequence 4 (Trs4) in spermatogenesis. [Masters Thesis]. University of Hong Kong; 2011. Available from: So, K. [蘇錦熙]. (2011). A study on the role of temperature repressed sequence 4 (Trs4) in spermatogenesis. (Thesis). University of Hong Kong, Pokfulam, Hong Kong SAR. Retrieved from http://dx.doi.org/10.5353/th_b4731070 ; http://dx.doi.org/10.5353/th_b4731070 ; http://hdl.handle.net/10722/174371

Hong Kong University of Science and Technology

9. Xiao, Nan. Role of PICK1 in acrosome formation and male fertility.

Degree: 2009, Hong Kong University of Science and Technology

URL: https://doi.org/10.14711/thesis-b1070232 ; http://repository.ust.hk/ir/bitstream/1783.1-6203/1/th_redirect.html

► Protein interacting with C kinase 1 (PICK1) is a higly conserved peripheral membrane protein involved in protein trafficking, a function that has been well characterized… (more)

Subjects/Keywords: Spermatogenesis; Protein kinases; Infertility, Male

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APA (6^th Edition):

Xiao, N. (2009). Role of PICK1 in acrosome formation and male fertility. (Thesis). Hong Kong University of Science and Technology. Retrieved from https://doi.org/10.14711/thesis-b1070232 ; http://repository.ust.hk/ir/bitstream/1783.1-6203/1/th_redirect.html

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Xiao, Nan. “Role of PICK1 in acrosome formation and male fertility.” 2009. Thesis, Hong Kong University of Science and Technology. Accessed December 15, 2019. https://doi.org/10.14711/thesis-b1070232 ; http://repository.ust.hk/ir/bitstream/1783.1-6203/1/th_redirect.html.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Xiao, Nan. “Role of PICK1 in acrosome formation and male fertility.” 2009. Web. 15 Dec 2019.

Vancouver:

Xiao N. Role of PICK1 in acrosome formation and male fertility. [Internet] [Thesis]. Hong Kong University of Science and Technology; 2009. [cited 2019 Dec 15]. Available from: https://doi.org/10.14711/thesis-b1070232 ; http://repository.ust.hk/ir/bitstream/1783.1-6203/1/th_redirect.html.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Xiao N. Role of PICK1 in acrosome formation and male fertility. [Thesis]. Hong Kong University of Science and Technology; 2009. Available from: https://doi.org/10.14711/thesis-b1070232 ; http://repository.ust.hk/ir/bitstream/1783.1-6203/1/th_redirect.html

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Hong Kong

10. Mok, Ka-wai. Blood testis barrier: its biology and significance in spermatogenesis.

Degree: PhD, 2012, University of Hong Kong

URL: Mok, K. [莫嘉維]. (2012). Blood testis barrier : its biology and significance in spermatogenesis. (Thesis). University of Hong Kong, Pokfulam, Hong Kong SAR. Retrieved from http://dx.doi.org/10.5353/th_b4979950 ; http://dx.doi.org/10.5353/th_b4979950 ; http://hdl.handle.net/10722/181516

► Spermatogenesis takes place in the seminiferous epithelium and it is a tightly regulated process that produces spermatozoa from spermatogonia. During spermatogenesis, germ cells have to… (more)

▼ Spermatogenesis takes place in the seminiferous epithelium and it is a tightly regulated process that produces spermatozoa from spermatogonia. During spermatogenesis, germ cells have to traverse the seminiferous epithelium, from basal to adluminal compartment and finally reach the luminal edge of the seminiferous tubules at spermiation. During the transit of germ cells, they have to get across the blood-testis barrier (BTB), which is formed by adjacent Sertoli cells. Thus, although BTB is considered as one of the tightest blood-tissue barrier, the BTB undergoes cyclic restructuring to “open” transiently for the translocation of germ cells. However, the integrity of the BTB has to remain intact as the BTB is essential for the developing germ cells behind the barrier. For example, the BTB serves as an immunological barrier to “seal” developing germ cells from the systemic circulation. Since how the BTB restructuring is regulated remains elusive, the study herein aims to provide some information regarding to this events. The importance of the BTB to spermatogenesis was demonstrated by treating rats with 50 (lowdose) or 250 mg/kg b.w (high-dose) of adjudin. Although the BTB of rats was perturbed in both groups at week 6 post treatment, as shown by an in vivo BTB functional assay, the BTB of the low-dose group was found to have “resealed” at week 20 whereas the BTB of the high-dose group remained disrupted. Besides, despite almost all germ cells were depleted in both group of rats upon week 2 post treatment, spermatogonia were still present in the testis of rats no matter high- or low-dose of adjudin was used. However, spermatogenesis only recovered in low-dose treated group, which have an intact BTB. This suggests that after spermatogenesis is disrupted, its regeneration of spermatogenesis needs more than the existence of spermatogonia in which an intact BTB is required. After demonstrating the necessity of the BTB for spermatogenesis, the next question I addressed was how its restructuring was modulated. The involvement of mammalian target of rapamycin (mTOR) in manipulating the BTB was investigated. mTOR is able to form two distinct signaling complexes namely mTOR complex 1 (mTORC1) or mTORC2 by assembling with raptor or rictor, respectively. rpS6, which is a downstream molecule of mTORC1 was activated specifically during BTB restructuring and knockdown of rpS6 in cultured Sertoli cells was able to promote the TJ-barrier by inducing deposition of F-actin and BTB proteins at the cell-cell interface, suggesting the role of phosphorylated rpS6 is to “open” the BTB for the transit of spermatocytes. Moreover, the knockdown of rictor led to perturbation of TJ-barrier formed by cultured Sertoli cells via a PKC-α depending actin reorganization, causing internalization of BTB proteins. This indicates mTORC2 is necessary for the maintenance of the BTB and hence the two mTOR complexes work antagonistically to regulate the BTB in which mTORC1 is activated to promote the BTB restructuring while the expression of mTORC2 is… Advisors/Committee Members: Lee, WWM.

Subjects/Keywords: Spermatogenesis in animals.; Testis - Physiology.

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APA (6^th Edition):

Mok, K. (2012). Blood testis barrier: its biology and significance in spermatogenesis. (Doctoral Dissertation). University of Hong Kong. Retrieved from Mok, K. [莫嘉維]. (2012). Blood testis barrier : its biology and significance in spermatogenesis. (Thesis). University of Hong Kong, Pokfulam, Hong Kong SAR. Retrieved from http://dx.doi.org/10.5353/th_b4979950 ; http://dx.doi.org/10.5353/th_b4979950 ; http://hdl.handle.net/10722/181516

Chicago Manual of Style (16^th Edition):

Mok, Ka-wai. “Blood testis barrier: its biology and significance in spermatogenesis.” 2012. Doctoral Dissertation, University of Hong Kong. Accessed December 15, 2019. Mok, K. [莫嘉維]. (2012). Blood testis barrier : its biology and significance in spermatogenesis. (Thesis). University of Hong Kong, Pokfulam, Hong Kong SAR. Retrieved from http://dx.doi.org/10.5353/th_b4979950 ; http://dx.doi.org/10.5353/th_b4979950 ; http://hdl.handle.net/10722/181516.

MLA Handbook (7^th Edition):

Mok, Ka-wai. “Blood testis barrier: its biology and significance in spermatogenesis.” 2012. Web. 15 Dec 2019.

Vancouver:

Mok K. Blood testis barrier: its biology and significance in spermatogenesis. [Internet] [Doctoral dissertation]. University of Hong Kong; 2012. [cited 2019 Dec 15]. Available from: Mok, K. [莫嘉維]. (2012). Blood testis barrier : its biology and significance in spermatogenesis. (Thesis). University of Hong Kong, Pokfulam, Hong Kong SAR. Retrieved from http://dx.doi.org/10.5353/th_b4979950 ; http://dx.doi.org/10.5353/th_b4979950 ; http://hdl.handle.net/10722/181516.

Council of Science Editors:

Mok K. Blood testis barrier: its biology and significance in spermatogenesis. [Doctoral Dissertation]. University of Hong Kong; 2012. Available from: Mok, K. [莫嘉維]. (2012). Blood testis barrier : its biology and significance in spermatogenesis. (Thesis). University of Hong Kong, Pokfulam, Hong Kong SAR. Retrieved from http://dx.doi.org/10.5353/th_b4979950 ; http://dx.doi.org/10.5353/th_b4979950 ; http://hdl.handle.net/10722/181516

Cornell University

11. Sartain, Caroline. Rna Regulation During Gametogenesis And Triggers Of Egg Activation In Drosophila Melanogaster .

Degree: 2013, Cornell University

URL: http://hdl.handle.net/1813/34218

► One of the most critical transitory periods during development occurs when an oocyte prepares to undergo embryogenesis, in a process called "egg activation". In many… (more)

▼ One of the most critical transitory periods during development occurs when an oocyte prepares to undergo embryogenesis, in a process called "egg activation". In many organisms, the oocyte has been at rest in the ovary, stored in a dormant state for a period ranging from a number of days (as in flies) to several decades (as in humans). Upon egg activation, this dormant egg must suddenly undergo several major cellular and molecular changes that prepare it for embryogenesis, including restructuring of the vitelline membrane, completion of meiosis, and changes to mRNA and protein pools. Where most organisms require a fertilizing sperm to set off these events, fruitflies are unique in that egg activation occurs prior to requirement of fertilization. I have used Drosophila melanogaster as a model system to dissect apart some key events of egg activation that are driven solely by the maternal components of the oocyte. In most animals, the initial trigger from the sperm sparks a calcium wave that traverses through the oocyte, acting as a message to kick off previously silenced biochemical pathways. For many years, it was unknown whether calcium played a role in egg activation in Drosophila. I have discovered that although Drosophila oocytes do not require a fertilizing sperm, a calcium wave does occur during egg activation. I have characterized the dynamics of this calcium influx using live imaging of Drosophila oocytes undergoing in vitro egg activation. Furthermore, I found that the calcium flux is not dependent upon internal stores but depends on extracellular calcium concentrations. Additionally, I have determined that the extracellular calcium likely enters the oocyte through mechanosensory TRP channels. Downstream of the calcium signal, there is major turnover of the maternallystored mRNAs within the oocyte. I have examined the phenotype and transcriptome changes in eggs from mothers carrying the prage mutation, an allele previously uncharacterized in egg activation. I have also performed microarray analysis of the global set of transcripts that undergo cytoplasmic polyadenylation by WISPY at egg activation. These data taken together give us a big-picture view of the global changes occurring in the mRNA pool during egg activation. Advisors/Committee Members: Goldberg, Michael Lewis (committeeMember), Liu, Jun (committeeMember).

Subjects/Keywords: Drosophila; egg activation; spermatogenesis

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APA (6^th Edition):

Sartain, C. (2013). Rna Regulation During Gametogenesis And Triggers Of Egg Activation In Drosophila Melanogaster . (Thesis). Cornell University. Retrieved from http://hdl.handle.net/1813/34218

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Sartain, Caroline. “Rna Regulation During Gametogenesis And Triggers Of Egg Activation In Drosophila Melanogaster .” 2013. Thesis, Cornell University. Accessed December 15, 2019. http://hdl.handle.net/1813/34218.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Sartain, Caroline. “Rna Regulation During Gametogenesis And Triggers Of Egg Activation In Drosophila Melanogaster .” 2013. Web. 15 Dec 2019.

Vancouver:

Sartain C. Rna Regulation During Gametogenesis And Triggers Of Egg Activation In Drosophila Melanogaster . [Internet] [Thesis]. Cornell University; 2013. [cited 2019 Dec 15]. Available from: http://hdl.handle.net/1813/34218.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Sartain C. Rna Regulation During Gametogenesis And Triggers Of Egg Activation In Drosophila Melanogaster . [Thesis]. Cornell University; 2013. Available from: http://hdl.handle.net/1813/34218

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Southern Illinois University

12. Roewer, Jesse F. CONSEQUENCE OF PREMATURE AND CHRONIC LUTEINIZING HORMONE RECEPTOR ACTIVATION ON TESTICULAR SPERMATOGENIC CELL DEVELOPMENT.

Degree: MS, Molecular Cellular and Systemic Physiology, 2010, Southern Illinois University

URL: http://opensiuc.lib.siu.edu/theses/253

► Luteinizing hormone (LH), one of the two gonadotropin hormones released from the anterior pituitary gland, binds to its receptor (LHR) in the gonads to… (more)

▼ Luteinizing hormone (LH), one of the two gonadotropin hormones released from the anterior pituitary gland, binds to its receptor (LHR) in the gonads to stimulate steroid hormone production, in addition to ovulation and gametogenesis. Mutations of the receptors amino acid sequence have the ability to either constitutively activate or inactivate it. All activating mutations result in male-limited precocious puberty. Males with this condition undergo puberty around 4 years of age, and have a premature elevation in testosterone levels and premature skeletal development. In order to understand how chronic ligand-mediated activation of the LHR affects gonadal development and function, a mouse model expressing a yoked hormone-receptor (YHR) complex, engineered by covalently linking the hormone human chorionic gonadotropin to the rat LHR, has been studied. YHR+ males have prepubertally elevated testosterone and decreased gonadotropin levels, smaller testis, and smaller average seminiferous tubule diameters when compared to wild type (WT) animals. In a preliminary breeding study it was shown that YHR+ males were sub-fertile. Based the phenotype exhibited by the YHR+ mice, it was hypothesized that increased levels of testosterone in addition to decreased gonadotropin hormone levels in neonatal and prepubertal mice that results from premature activation of the luteinizing hormone receptor causes spermatogenesis to be impaired. The first objective of this study was to determine if there was a difference in the testicular germ cell and Sertoli cell populations in the WT and YHR+ animals using flow cytometry and systematic Sertoli cell counting, respectively. There was no difference in the Sertoli cell population between YHR+ animals and WT controls, but there were significantly fewer total germ cells in YHR+ animals at 10 days and from 4 weeks through adulthood. The second objective was to calculate the daily sperm production in the testis and epididymis of WT and YHR+ animals in order to determine if there is a further decrease in the total sperm count due to an epididymal dysfunction. Interestingly, there were significantly fewer sperm calculated in the caput/corpus region of the epididymis in YHR+ males at 12 weeks of age, but not in the testis and cauda epididymis. Furthermore, the daily sperm production in WT and YHR+ mice at 16 weeks of age were not significantly different. The final objective was to determine if the decrease in germ cells observed in YHR+ animals is the result of decreased proliferation or an increase in either germ cell or Sertoli cell apoptosis. Quantification of germ cell and Sertoli cell proliferation revealed no significant difference between the WT and YHR+ animals. Similar findings were found after quantification of apoptotic germ cell and Sertoli cells. Taken together, these data suggest that premature elevation in testosterone and persistently lower levels of circulating follicle stimulating hormone (FSH) are affecting Sertoli cell function, which is causing a reduced germ cell to Sertoli cell… Advisors/Committee Members: Narayan, Dr. Prema.

Subjects/Keywords: Germ cells; Reproduction; Spermatogenesis

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APA (6^th Edition):

Roewer, J. F. (2010). CONSEQUENCE OF PREMATURE AND CHRONIC LUTEINIZING HORMONE RECEPTOR ACTIVATION ON TESTICULAR SPERMATOGENIC CELL DEVELOPMENT. (Masters Thesis). Southern Illinois University. Retrieved from http://opensiuc.lib.siu.edu/theses/253

Chicago Manual of Style (16^th Edition):

Roewer, Jesse F. “CONSEQUENCE OF PREMATURE AND CHRONIC LUTEINIZING HORMONE RECEPTOR ACTIVATION ON TESTICULAR SPERMATOGENIC CELL DEVELOPMENT.” 2010. Masters Thesis, Southern Illinois University. Accessed December 15, 2019. http://opensiuc.lib.siu.edu/theses/253.

MLA Handbook (7^th Edition):

Roewer, Jesse F. “CONSEQUENCE OF PREMATURE AND CHRONIC LUTEINIZING HORMONE RECEPTOR ACTIVATION ON TESTICULAR SPERMATOGENIC CELL DEVELOPMENT.” 2010. Web. 15 Dec 2019.

Vancouver:

Roewer JF. CONSEQUENCE OF PREMATURE AND CHRONIC LUTEINIZING HORMONE RECEPTOR ACTIVATION ON TESTICULAR SPERMATOGENIC CELL DEVELOPMENT. [Internet] [Masters thesis]. Southern Illinois University; 2010. [cited 2019 Dec 15]. Available from: http://opensiuc.lib.siu.edu/theses/253.

Council of Science Editors:

Roewer JF. CONSEQUENCE OF PREMATURE AND CHRONIC LUTEINIZING HORMONE RECEPTOR ACTIVATION ON TESTICULAR SPERMATOGENIC CELL DEVELOPMENT. [Masters Thesis]. Southern Illinois University; 2010. Available from: http://opensiuc.lib.siu.edu/theses/253

University of Newcastle

13. Lv , Yonggang. Study the male reproductive biology of a marine invertebrate Galeolaria caespitosa and investigate its potential as a bio-indicator species of coastal marine pollution.

Degree: PhD, 2016, University of Newcastle

URL: http://hdl.handle.net/1959.13/1332731

►

Research Doctorate - Doctor of Philosophy (PhD)

The history of marine pollution can be traced back to the very beginning of human civilisation. Nevertheless, this… (more)

▼

Research Doctorate - Doctor of Philosophy (PhD)

The history of marine pollution can be traced back to the very beginning of human civilisation. Nevertheless, this issue did not raise serious concern until a threshold level was reached with devastating consequences for the marine ecosystem. In recent years, bio-indicator species have been gradually embraced as one of the most effective approaches to predict, monitor and assess marine contamination. The statistics acquired from biomonitoring not only facilitate determination of the biological significance of man-made hazardous substances on marine ecosystems but also establish an important foundation for marine wildlife conservation and public health management. The focal species in this PhD project is an Australian native marine invertebrate Galeolaria caespitosa, which is recognised as a potential bio-indicator species for coastal marine pollution and a model organism for use in laboratory toxicity tests, due to a number of remarkable life history characteristics it exhibits. According to a range of recent studies, the gametes of G. caespitosa, particularly its spermatozoa, and the processes of fertilisation, embryogenesis and larval development were susceptible to marine contaminants and could be utilised as sensitive indicators of pollutants in the immediate environment. Nevertheless, the reproductive biology of this species, particular the male reproductive system, has only been superficially described. This knowledge gap has created an opportunity to undertake a comprehensive investigation of reproduction and embryonic development in this species including the sensitivity of these processes to common environmental contaminants. (1) Utilising serial histological sections and electron microscopic techniques, the male reproductive system in G. caespitosa was reconstructed and the general pattern of spermatogenesis was established. This study for the first time established a comprehensive model of male reproductive system in polychaetes, with the function of each compartment being elucidated in detail. In addition, clear similarities were found between the male reproductive system in this simple invertebrate and human beings in terms of the structure and function.(2) As the differentiation of round spermatids in G. caespitosa took place in the germinal fluid without any physical support from nurse cells, spermiogenesis in this species was regarded as a convenient model for studying the underlying mechanisms of this complex differentiation process. Therefore, spermiogenesis was analysed in detail at the ultrastructural level and a preliminary study was performed to reproduce the differentiation of spermatids in vitro. This in vitro study revealed that the regulators of spermiogenesis in G. caespitosa were gender-specific and involved proteinaceous constituents thereby providing a basis for future studies addressing the molecular regulation of this complex differentiation process. (3) Exposure of spermatozoa to low…

Advisors/Committee Members: University of Newcastle. Faculty of Science & Information Technology, School of Environmental and Life Sciences.

Subjects/Keywords: spermatogenesis; embryogenesis; plasticizer; bio-indicator

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APA (6^th Edition):

Lv , Y. (2016). Study the male reproductive biology of a marine invertebrate Galeolaria caespitosa and investigate its potential as a bio-indicator species of coastal marine pollution. (Doctoral Dissertation). University of Newcastle. Retrieved from http://hdl.handle.net/1959.13/1332731

Chicago Manual of Style (16^th Edition):

Lv , Yonggang. “Study the male reproductive biology of a marine invertebrate Galeolaria caespitosa and investigate its potential as a bio-indicator species of coastal marine pollution.” 2016. Doctoral Dissertation, University of Newcastle. Accessed December 15, 2019. http://hdl.handle.net/1959.13/1332731.

MLA Handbook (7^th Edition):

Lv , Yonggang. “Study the male reproductive biology of a marine invertebrate Galeolaria caespitosa and investigate its potential as a bio-indicator species of coastal marine pollution.” 2016. Web. 15 Dec 2019.

Vancouver:

Lv Y. Study the male reproductive biology of a marine invertebrate Galeolaria caespitosa and investigate its potential as a bio-indicator species of coastal marine pollution. [Internet] [Doctoral dissertation]. University of Newcastle; 2016. [cited 2019 Dec 15]. Available from: http://hdl.handle.net/1959.13/1332731.

Council of Science Editors:

Lv Y. Study the male reproductive biology of a marine invertebrate Galeolaria caespitosa and investigate its potential as a bio-indicator species of coastal marine pollution. [Doctoral Dissertation]. University of Newcastle; 2016. Available from: http://hdl.handle.net/1959.13/1332731

University of Guelph

14. Giffin, Jennifer L. Determinants of Testicular Echotexture in the Sexually Immature Ram Lamb .

Degree: 2015, University of Guelph

URL: https://atrium.lib.uoguelph.ca/xmlui/handle/10214/8662

► Throughout sexual maturation, dynamic changes in testicular macro- and microstructure and reproductive hormone levels occur. Future adult reproductive capability is critically dependent on these changes;… (more)

▼ Throughout sexual maturation, dynamic changes in testicular macro- and microstructure and reproductive hormone levels occur. Future adult reproductive capability is critically dependent on these changes; therefore, regular monitoring of pubertal testicular development is desirable. However, conventional methods of assessment do not permit the frequent and non-invasive examination of testicular function. Recently, scrotal ultrasonography in conjunction with computer-assisted image analysis has emerged as a potential non-invasive alternative for male reproductive assessment. In this procedure, testicular echotexture, or the appearance of the ultrasonogram, is objectively quantified on the basis of brightness or intensity of the minute picture elements, or pixels, comprising the image. In general, testicular pixel intensity increases with age throughout sexual maturation; however, periodic fluctuations occur. Changes in testicular echotexture are related to microstructural attributes of the testes and reproductive hormone secretion, but reports on these relationships have been inconsistent. Therefore, the overall objective of the studies presented in this thesis was to investigate how testicular echotexture and its associations with testicular histomorphology and endocrine profiles may be influenced by various factors including: i) scrotal/testicular integument; ii) blood flow/content; iii) stage of development; and iv) altered spermatogenic onset. Serial scrotal ultrasonograms, testicular biopsies or castrations and blood samples were collected from early postnatal or peripubescent ram lambs and analyzed to obtain echotextural, histomorphological and endocrine data, respectively. Testicular echotextural attributes were influenced by the presence of the scrotal and testicular integument, the stage of spermatogenic development (mitotic vs. post-mitotic) and the amount of germ cell heterogeneity present with or without retinoic acid exposure, but not by blood flow or content. Tubular and luminal size of the seminiferous tubules could be predicted from testicular pixel intensity using a simple linear regression model and demonstrated a moderate strength correlation with testicular echotextural parameters, as did a number of cellular and sub-cellular histomorphological attributes and reproductive hormone levels. These results clearly demonstrate the ability of testicular echotexture to detect experimentally and clinically relevant markers of testicular function. Computerized image analysis of scrotal ultrasonograms holds promise as a non-invasive diagnostic and prognostic tool and novel monitoring device of testicular histophysiology. Advisors/Committee Members: Bartlewski, Pawel M (advisor).

Subjects/Keywords: testis; ultrasound; puberty; spermatogenesis

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APA (6^th Edition):

Giffin, J. L. (2015). Determinants of Testicular Echotexture in the Sexually Immature Ram Lamb . (Thesis). University of Guelph. Retrieved from https://atrium.lib.uoguelph.ca/xmlui/handle/10214/8662

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Giffin, Jennifer L. “Determinants of Testicular Echotexture in the Sexually Immature Ram Lamb .” 2015. Thesis, University of Guelph. Accessed December 15, 2019. https://atrium.lib.uoguelph.ca/xmlui/handle/10214/8662.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Giffin, Jennifer L. “Determinants of Testicular Echotexture in the Sexually Immature Ram Lamb .” 2015. Web. 15 Dec 2019.

Vancouver:

Giffin JL. Determinants of Testicular Echotexture in the Sexually Immature Ram Lamb . [Internet] [Thesis]. University of Guelph; 2015. [cited 2019 Dec 15]. Available from: https://atrium.lib.uoguelph.ca/xmlui/handle/10214/8662.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Giffin JL. Determinants of Testicular Echotexture in the Sexually Immature Ram Lamb . [Thesis]. University of Guelph; 2015. Available from: https://atrium.lib.uoguelph.ca/xmlui/handle/10214/8662

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Michigan State University

15. Poirier, Gary Raymond, 1938-. An ultrastructural study of spermatogenesis in two species of Rana.

Degree: PhD, Department of Zoology, 1970, Michigan State University

URL: http://etd.lib.msu.edu/islandora/object/etd:37231

Subjects/Keywords: Spermatogenesis; Frogs

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Poirier, Gary Raymond, 1. (1970). An ultrastructural study of spermatogenesis in two species of Rana. (Doctoral Dissertation). Michigan State University. Retrieved from http://etd.lib.msu.edu/islandora/object/etd:37231

Chicago Manual of Style (16^th Edition):

Poirier, Gary Raymond, 1938-. “An ultrastructural study of spermatogenesis in two species of Rana.” 1970. Doctoral Dissertation, Michigan State University. Accessed December 15, 2019. http://etd.lib.msu.edu/islandora/object/etd:37231.

MLA Handbook (7^th Edition):

Poirier, Gary Raymond, 1938-. “An ultrastructural study of spermatogenesis in two species of Rana.” 1970. Web. 15 Dec 2019.

Vancouver:

Poirier, Gary Raymond 1. An ultrastructural study of spermatogenesis in two species of Rana. [Internet] [Doctoral dissertation]. Michigan State University; 1970. [cited 2019 Dec 15]. Available from: http://etd.lib.msu.edu/islandora/object/etd:37231.

Council of Science Editors:

Poirier, Gary Raymond 1. An ultrastructural study of spermatogenesis in two species of Rana. [Doctoral Dissertation]. Michigan State University; 1970. Available from: http://etd.lib.msu.edu/islandora/object/etd:37231

16. Ruggiu, Matteo. The function of DAZL and RBM, two candidate fertility genes.

Degree: PhD, 1999, Open University

URL: https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.300014

Subjects/Keywords: 572.8; Spermatogenesis

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APA (6^th Edition):

Ruggiu, M. (1999). The function of DAZL and RBM, two candidate fertility genes. (Doctoral Dissertation). Open University. Retrieved from https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.300014

Chicago Manual of Style (16^th Edition):

Ruggiu, Matteo. “The function of DAZL and RBM, two candidate fertility genes.” 1999. Doctoral Dissertation, Open University. Accessed December 15, 2019. https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.300014.

MLA Handbook (7^th Edition):

Ruggiu, Matteo. “The function of DAZL and RBM, two candidate fertility genes.” 1999. Web. 15 Dec 2019.

Vancouver:

Ruggiu M. The function of DAZL and RBM, two candidate fertility genes. [Internet] [Doctoral dissertation]. Open University; 1999. [cited 2019 Dec 15]. Available from: https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.300014.

Council of Science Editors:

Ruggiu M. The function of DAZL and RBM, two candidate fertility genes. [Doctoral Dissertation]. Open University; 1999. Available from: https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.300014

Australian National University

17. Anuar, Nur Diana. Using TALENs to Knockout H2A.Lap1 Function in Mice .

Degree: 2018, Australian National University

URL: http://hdl.handle.net/1885/143927

► The Tremethick laboratory in 2012 discovered a new mouse histone variant, which was designated H2A.Lap1 (Lack of an acidic patch), which, in the adult, is… (more)

▼ The Tremethick laboratory in 2012 discovered a new mouse histone variant, which was designated H2A.Lap1 (Lack of an acidic patch), which, in the adult, is uniquely expressed in the testis and the brain. It was proposed that in the testis, H2A.Lap1 is involved in a new mechanism of gene regulation whereby H2A.Lap1 co-ordinately activates the expression of many genes by being targeted to and directly opening the chromatin region encompassing their transcription start site (TSS). More recently, this laboratory also identified H2A.Lap1 as a component of the intron-exon boundary of active genes expressed in the testis and the brain. These observations suggested a role in transcription and splicing but all the experiments to date have been correlative in nature. Therefore, the major aim of this thesis was to generate an in vivo mouse knockout model in order to directly test the importance of H2A.Lap1. However, the generation of a H2A.Lap1 knockout (KO) mouse was potentially a technically challenging feat because the H2A.Lap1 protein is expressed from three different genes (all located on the X chromosome). In this thesis, we report a simplified TALEN approach that achieved this goal whereby we used one pair of TALENs to simultaneously disrupt the three gene copies of H2A.Lap1. To our knowledge, this has not been done before. Importantly, bioinformatics analyses and wet-lab validation on exome sequencing data of our KO mice did not reveal any TALEN-induced off-target mutations, proving the reliability of this genome editing approach in generating a H2A.Lap1-specific KO model. Since H2A.Lap1 is predominantly expressed in the testis, we first explored whether are H2A.Lap1 KO mice are fertile. Our results showed that the loss of H2A.Lap1 caused a mild subfertility phenotype that manifested itself in smaller litter sizes when the hemizygous KO males (H2A.Lap1 -/Y) were mated with wild type females (H2A.Lap1 +/+). Given that these hemizygous KO mice are still able to reproduce despite the absence of H2A.Lap1 suggested that H2A.Lap1 is important but not essential for fertility. Alternatively though, H2A.Lap1 may be critical but compensatory epigenetic mechanisms may come into play in its absence. To test this hypothesis, I examined the immunofluorescence distribution of several active promoter-associated histone post translational modifications (PTMs) in germ cells, as well as investigating another H2A.Lap1 like histone variant, H2A.L.2 (expressed at latter spermatogenesis stages compared to H2A.Lap1) in H2A.Lap1 -/Y mice and their wt siblings. The immunostaining analyses showed that, in comparison to the wt mice, H3K4me3 become more enriched in the euchromatin of round spermatids of KO mice, implying a possible involvement of this PTM in a compensation mechanism. In addition, and most…

Subjects/Keywords: epigenetics; chromatin; histone variants; spermatogenesis

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APA (6^th Edition):

Anuar, N. D. (2018). Using TALENs to Knockout H2A.Lap1 Function in Mice . (Thesis). Australian National University. Retrieved from http://hdl.handle.net/1885/143927

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Anuar, Nur Diana. “Using TALENs to Knockout H2A.Lap1 Function in Mice .” 2018. Thesis, Australian National University. Accessed December 15, 2019. http://hdl.handle.net/1885/143927.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Anuar, Nur Diana. “Using TALENs to Knockout H2A.Lap1 Function in Mice .” 2018. Web. 15 Dec 2019.

Vancouver:

Anuar ND. Using TALENs to Knockout H2A.Lap1 Function in Mice . [Internet] [Thesis]. Australian National University; 2018. [cited 2019 Dec 15]. Available from: http://hdl.handle.net/1885/143927.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Anuar ND. Using TALENs to Knockout H2A.Lap1 Function in Mice . [Thesis]. Australian National University; 2018. Available from: http://hdl.handle.net/1885/143927

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

The Ohio State University

18. Kumar, Devi. Ultrastructure of the mature spermatozoa and the process of spermiogenesis in the cockroach, Nauphoeta cinerea (Dictyoptera: Blattaria: Blaberidae).

Degree: PhD, Graduate School, 1976, The Ohio State University

URL: http://rave.ohiolink.edu/etdc/view?acc_num=osu1487003143385501

Subjects/Keywords: Biology; Cockroaches; Spermatogenesis

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APA (6^th Edition):

Kumar, D. (1976). Ultrastructure of the mature spermatozoa and the process of spermiogenesis in the cockroach, Nauphoeta cinerea (Dictyoptera: Blattaria: Blaberidae). (Doctoral Dissertation). The Ohio State University. Retrieved from http://rave.ohiolink.edu/etdc/view?acc_num=osu1487003143385501

Chicago Manual of Style (16^th Edition):

Kumar, Devi. “Ultrastructure of the mature spermatozoa and the process of spermiogenesis in the cockroach, Nauphoeta cinerea (Dictyoptera: Blattaria: Blaberidae).” 1976. Doctoral Dissertation, The Ohio State University. Accessed December 15, 2019. http://rave.ohiolink.edu/etdc/view?acc_num=osu1487003143385501.

MLA Handbook (7^th Edition):

Kumar, Devi. “Ultrastructure of the mature spermatozoa and the process of spermiogenesis in the cockroach, Nauphoeta cinerea (Dictyoptera: Blattaria: Blaberidae).” 1976. Web. 15 Dec 2019.

Vancouver:

Kumar D. Ultrastructure of the mature spermatozoa and the process of spermiogenesis in the cockroach, Nauphoeta cinerea (Dictyoptera: Blattaria: Blaberidae). [Internet] [Doctoral dissertation]. The Ohio State University; 1976. [cited 2019 Dec 15]. Available from: http://rave.ohiolink.edu/etdc/view?acc_num=osu1487003143385501.

Council of Science Editors:

Kumar D. Ultrastructure of the mature spermatozoa and the process of spermiogenesis in the cockroach, Nauphoeta cinerea (Dictyoptera: Blattaria: Blaberidae). [Doctoral Dissertation]. The Ohio State University; 1976. Available from: http://rave.ohiolink.edu/etdc/view?acc_num=osu1487003143385501

University of Kentucky

19. Keesling, David C. INVESTIGATING THE PED PROTEIN AND ITS EFFECT ON TRANSLATIONAL CONTROL IN DROSOPHILA MELANOGASTER SPERMATOGENESIS.

Degree: 2012, University of Kentucky

URL: http://uknowledge.uky.edu/biology_etds/2

► Inactive mutants of the ped gene cause two phenotypes in Drosophila melanogaster: male sterility and the early translation of DHODH within spermatogenesis. Investigation of the… (more)

Subjects/Keywords: translational control; deubiquitination; spermatogenesis; otubain; proteolysis; Biology

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APA (6^th Edition):

Keesling, D. C. (2012). INVESTIGATING THE PED PROTEIN AND ITS EFFECT ON TRANSLATIONAL CONTROL IN DROSOPHILA MELANOGASTER SPERMATOGENESIS. (Masters Thesis). University of Kentucky. Retrieved from http://uknowledge.uky.edu/biology_etds/2

Chicago Manual of Style (16^th Edition):

Keesling, David C. “INVESTIGATING THE PED PROTEIN AND ITS EFFECT ON TRANSLATIONAL CONTROL IN DROSOPHILA MELANOGASTER SPERMATOGENESIS.” 2012. Masters Thesis, University of Kentucky. Accessed December 15, 2019. http://uknowledge.uky.edu/biology_etds/2.

MLA Handbook (7^th Edition):

Keesling, David C. “INVESTIGATING THE PED PROTEIN AND ITS EFFECT ON TRANSLATIONAL CONTROL IN DROSOPHILA MELANOGASTER SPERMATOGENESIS.” 2012. Web. 15 Dec 2019.

Vancouver:

Keesling DC. INVESTIGATING THE PED PROTEIN AND ITS EFFECT ON TRANSLATIONAL CONTROL IN DROSOPHILA MELANOGASTER SPERMATOGENESIS. [Internet] [Masters thesis]. University of Kentucky; 2012. [cited 2019 Dec 15]. Available from: http://uknowledge.uky.edu/biology_etds/2.

Council of Science Editors:

Keesling DC. INVESTIGATING THE PED PROTEIN AND ITS EFFECT ON TRANSLATIONAL CONTROL IN DROSOPHILA MELANOGASTER SPERMATOGENESIS. [Masters Thesis]. University of Kentucky; 2012. Available from: http://uknowledge.uky.edu/biology_etds/2

Texas A&M University

20. Heninger, Noah Leland, III. The role of testicular germ cell apoptosis during equine spermatogenesis.

Degree: 2007, Texas A&M University

URL: http://hdl.handle.net/1969.1/4691

► Apoptosis in testicular germ cells has been demonstrated in many species. Features of apoptosis reported in other species were used to confirm use of the… (more)

▼ Apoptosis in testicular germ cells has been demonstrated in many species. Features of apoptosis reported in other species were used to confirm use of the TUNEL assay in stallion testes. Eight stallions with normal testicular size and semen quality were evaluated to determine the germ cell types and stages where apoptosis most commonly occurs. Mean numbers of TUNEL-positive germ cells per 100 Sertoli cell nuclei were highest in stages IV and V of the seminiferous epithelial cycle corresponding to meiotic divisions of primary spermatocytes and mitotic proliferation of B1 and B2 spermatogonia. Round and elongated spermatids were labeled less frequently by the TUNEL assay. To examine the relationships between germ cell apoptotic rate and spermatogenic efficiency, seminal traits were assessed to classify stallions into normal or reduced quality semen groups. Apoptotic rates were higher for stages IV-VI and stage VIII seminiferous tubules in stallions with reduced semen quality. Daily sperm production (DSP) per gram and per testis were lower for stallions with reduced semen quality. Regression analyses revealed negative linear relationships for germ cell apoptotic rate with DSP/g, DSP/testis, daily sperm output, progressively motile sperm and morphologically normal sperm in ejaculates. Mean circulating concentrations of inhibin were lower for stallions ejaculating reduced quality semen. Apoptotic rate was negatively correlated with concentrations of inhibin and estradiol-17b and positively correlated with concentrations of LH and FSH. To study germ cell apoptosis and formation of the Sertoli cell barrier during the initiation of spermatogenesis, tubule development was classified based on lumen score. Formation of a seminiferous tubule lumen was consistent with events leading to development of a Sertoli cell barrier. A primary wave of apoptosis removed early differentiating germ cell types prior to the formation of a tubule lumen facilitating both the formation of a tubule lumen and a Sertoli cell barrier. A second wave of apoptosis occurred after the formation of a lumen but before seminiferous tubule cross-sections contained a full complement of germ cells. In conclusion, apoptosis is an essential mechanism during normal spermatogenesis. Apoptosis also accounts for low numbers of normal sperm seen in the ejaculates of some stallions. Advisors/Committee Members: Forrest, David W. (advisor), Varner, Dickson D. (advisor), Blanchard, Terry L. (committee member), Johnson, Larry (committee member).

Subjects/Keywords: Apoptosis; spermatogenesis; stallion

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APA (6^th Edition):

Heninger, Noah Leland, I. (2007). The role of testicular germ cell apoptosis during equine spermatogenesis. (Thesis). Texas A&M University. Retrieved from http://hdl.handle.net/1969.1/4691

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Heninger, Noah Leland, III. “The role of testicular germ cell apoptosis during equine spermatogenesis.” 2007. Thesis, Texas A&M University. Accessed December 15, 2019. http://hdl.handle.net/1969.1/4691.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Heninger, Noah Leland, III. “The role of testicular germ cell apoptosis during equine spermatogenesis.” 2007. Web. 15 Dec 2019.

Vancouver:

Heninger, Noah Leland I. The role of testicular germ cell apoptosis during equine spermatogenesis. [Internet] [Thesis]. Texas A&M University; 2007. [cited 2019 Dec 15]. Available from: http://hdl.handle.net/1969.1/4691.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Heninger, Noah Leland I. The role of testicular germ cell apoptosis during equine spermatogenesis. [Thesis]. Texas A&M University; 2007. Available from: http://hdl.handle.net/1969.1/4691

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Kashmir

21. Safaya, Phoola. Studies on Chromosomes of Kashmir fishes with their number structure and karyotypes in Spermatogenesis of twelve fishes;.

Degree: 2014, University of Kashmir

URL: http://shodhganga.inflibnet.ac.in/handle/10603/20413

newline

Advisors/Committee Members: Das, S. M..

Subjects/Keywords: Chromosomes-Spermatogenesis-Kashmir

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APA (6^th Edition):

Safaya, P. (2014). Studies on Chromosomes of Kashmir fishes with their number structure and karyotypes in Spermatogenesis of twelve fishes;. (Thesis). University of Kashmir. Retrieved from http://shodhganga.inflibnet.ac.in/handle/10603/20413

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Safaya, Phoola. “Studies on Chromosomes of Kashmir fishes with their number structure and karyotypes in Spermatogenesis of twelve fishes;.” 2014. Thesis, University of Kashmir. Accessed December 15, 2019. http://shodhganga.inflibnet.ac.in/handle/10603/20413.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Safaya, Phoola. “Studies on Chromosomes of Kashmir fishes with their number structure and karyotypes in Spermatogenesis of twelve fishes;.” 2014. Web. 15 Dec 2019.

Vancouver:

Safaya P. Studies on Chromosomes of Kashmir fishes with their number structure and karyotypes in Spermatogenesis of twelve fishes;. [Internet] [Thesis]. University of Kashmir; 2014. [cited 2019 Dec 15]. Available from: http://shodhganga.inflibnet.ac.in/handle/10603/20413.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Safaya P. Studies on Chromosomes of Kashmir fishes with their number structure and karyotypes in Spermatogenesis of twelve fishes;. [Thesis]. University of Kashmir; 2014. Available from: http://shodhganga.inflibnet.ac.in/handle/10603/20413

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Cape Peninsula University of Technology

22. Aboua, Yapo Guillaume. The impact of organic hydroperoxides and a red palm oil supplemented diet on spermatogenesis, sperm function and sperm apoptosis .

Degree: 2009, Cape Peninsula University of Technology

URL: http://etd.cput.ac.za/handle/20.500.11838/1523

► Many environmental, physiological, and genetic factors have been shown to impair sperm function through oxidative damage. Oxidative stress (OS) arises as a consequence of excessive… (more)

▼ Many environmental, physiological, and genetic factors have been shown to impair sperm function through oxidative damage. Oxidative stress (OS) arises as a consequence of excessive reactive oxygen species (ROS) production and/or impaired antioxidant defence mechanisms. The decline in male reproductive health generated considerable public and scientific concerns about the possible role of environmental contaminants. A better understanding of how OS affects sperm function will be beneficial as it might help in the design of new and effective treatment strategies to combat the problem of increasing male subfertility. Studies have suggested that antioxidant nutrients and/or medicines play a protective role in human health. Crude red palm oil (RPO) is known to be the richest natural plant source of antioxidants such as carotenoids, tocopherols and metalloporpheryns. The aims of this study were twofold: (i) To establish an in vivo animal model of OS by exposing rat to organic hydroperoxide such as t-butyl hydroperoxide (tbHP) and cumene hydroperoxide (cHP) through repeated intraperitoneal injections that can be used for studying these effects on testicular tissue, epididymal sperm and sperm function as well as male reproductive parameters in general. (ii) To investigate the effects of a RPO supplemented diet on male reproductive parameters and tissue in animals exposed to OS. In the first part of the study, male Wistar rats aged 10-12 weeks were randomly placed in groups and received standard rat chow (SRC) and water ad lib. Animals were injected intraperitoneally with saline (0.5 ml), t-butyl hydroperoxide (5µM, 10µM, 20µM and 40µM; 0.5 ml) or cumene hydroperoxide cHP (2.5µM, 5µM, 10µM and 20µM; 0.5 ml) over a 60 day period. In the second part, male Wistar rats aged 10-12 weeks were placed randomly in three groups and fed with SRC. Group 1 received no supplement while the food of groups 2 and 3 were supplemented with 2 mL and 4 mL RPO (in 25 gm SRC/day) respectively. Each group was further divided into 3 subgroups and injected intraperitoneally daily with either saline, 10µM cHP or 20µM tbHP respectively. This was done for 5 consecutive days per week over a 60 day period. Sperm concentrations, and motility, lipid peroxidation, superoxide dismutase (SOD), catalase (CAT) and glutathione (GSH) activities as well as apoptosis were assessed.

Subjects/Keywords: Spermatogenesis; Spermatozoa – Physiology; Palm oil; Peroxides

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APA (6^th Edition):

Aboua, Y. G. (2009). The impact of organic hydroperoxides and a red palm oil supplemented diet on spermatogenesis, sperm function and sperm apoptosis . (Thesis). Cape Peninsula University of Technology. Retrieved from http://etd.cput.ac.za/handle/20.500.11838/1523

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Aboua, Yapo Guillaume. “The impact of organic hydroperoxides and a red palm oil supplemented diet on spermatogenesis, sperm function and sperm apoptosis .” 2009. Thesis, Cape Peninsula University of Technology. Accessed December 15, 2019. http://etd.cput.ac.za/handle/20.500.11838/1523.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Aboua, Yapo Guillaume. “The impact of organic hydroperoxides and a red palm oil supplemented diet on spermatogenesis, sperm function and sperm apoptosis .” 2009. Web. 15 Dec 2019.

Vancouver:

Aboua YG. The impact of organic hydroperoxides and a red palm oil supplemented diet on spermatogenesis, sperm function and sperm apoptosis . [Internet] [Thesis]. Cape Peninsula University of Technology; 2009. [cited 2019 Dec 15]. Available from: http://etd.cput.ac.za/handle/20.500.11838/1523.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Aboua YG. The impact of organic hydroperoxides and a red palm oil supplemented diet on spermatogenesis, sperm function and sperm apoptosis . [Thesis]. Cape Peninsula University of Technology; 2009. Available from: http://etd.cput.ac.za/handle/20.500.11838/1523

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Newcastle upon Tyne

23. Floros, Vasileios. An investigation of the role of Notch signalling in germ cell development using mouse embryonic stem cells.

Degree: PhD, 2013, University of Newcastle upon Tyne

URL: http://hdl.handle.net/10443/2282

Subjects/Keywords: 571.8; Proteins, Spermatogenesis

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APA (6^th Edition):

Floros, V. (2013). An investigation of the role of Notch signalling in germ cell development using mouse embryonic stem cells. (Doctoral Dissertation). University of Newcastle upon Tyne. Retrieved from http://hdl.handle.net/10443/2282

Chicago Manual of Style (16^th Edition):

Floros, Vasileios. “An investigation of the role of Notch signalling in germ cell development using mouse embryonic stem cells.” 2013. Doctoral Dissertation, University of Newcastle upon Tyne. Accessed December 15, 2019. http://hdl.handle.net/10443/2282.

MLA Handbook (7^th Edition):

Floros, Vasileios. “An investigation of the role of Notch signalling in germ cell development using mouse embryonic stem cells.” 2013. Web. 15 Dec 2019.

Vancouver:

Floros V. An investigation of the role of Notch signalling in germ cell development using mouse embryonic stem cells. [Internet] [Doctoral dissertation]. University of Newcastle upon Tyne; 2013. [cited 2019 Dec 15]. Available from: http://hdl.handle.net/10443/2282.

Council of Science Editors:

Floros V. An investigation of the role of Notch signalling in germ cell development using mouse embryonic stem cells. [Doctoral Dissertation]. University of Newcastle upon Tyne; 2013. Available from: http://hdl.handle.net/10443/2282

University of Hong Kong

24. Cao, Shanbo. Gene targeting to study a novel testis-specific gene Vad1.2 in spermatogenesis.

Degree: PhD, 2012, University of Hong Kong

URL: Cao, S. [曹善柏]. (2012). Gene targeting to study a novel testis-specific gene Vad1.2 in spermatogenesis. (Thesis). University of Hong Kong, Pokfulam, Hong Kong SAR. Retrieved from http://dx.doi.org/10.5353/th_b4979925 ; http://dx.doi.org/10.5353/th_b4979925 ; http://hdl.handle.net/10722/207896

► Spermatogenesis is regulated by steroid hormones which induce expression of various genes responsible for the growth, proliferation and differentiation of spermatogonia to form mature haploid… (more)

▼ Spermatogenesis is regulated by steroid hormones which induce expression of various genes responsible for the growth, proliferation and differentiation of spermatogonia to form mature haploid spermatozoa. The surrounding somatic cells including Leydig and Sertoli cells support the whole process in vivo. Previously, we used the post-vitamin A treated vitamin-A-deficient (PVA-VAD) rat model to study spermatogenesis, and identified 24 genes that are differentially up-regulated after retinol treatment. Vad1.2 is one of the up-regulated transcripts expressed in the rat testis from postnatal day 25. Vad1.2 transcript is localized to the round and elongating spermatids in the adult mouse testis. In silico analyses showed that Vad1.2 transcript is down-regulated in patients with teratozoospermia and non-obstructive azoospermia, suggesting that Vad1.2 may have important roles in spermatogenesis. However, how Vad1.2 affects spermatogenesis remains unclear. Therefore, the present study was designed to study the functional roles of Vad1.2 protein in mice using gene targeting approach and investigate the molecular changes in mice with Vad1.2 deficiency. Vad1.2 polyclonal antibody was raised against the full-length mouse Vad1.2 recombination protein and affinity purified. Vad1.2 protein was localized to the cytoplasm and flagellum of condensing spermatids, specifically to the fibrous sheath (FS) in cauda epididymal spermatozoa. Vad1.2 conditional knockout vector was constructed and used to generate Vad1.2 null mice. Vad1.2-/- male mice developed normally but were subfertile with reduced sperm count and motility. Vad1.2-/- male mice had smaller testis and higher incident of sloughing of immature germ cells into the seminiferous lumens when compared to the wild-type. Yet, the rates of germ cell proliferation and apoptosis were similar between the wild-type and the mutant testis. Interestingly approximately 50% of the mutant cauda epididymal spermatozoa showed deformed flagella and demonstrated structural defects typically associated with bending of flagellum at the principal piece or at the midpiece/principal piece junctions. The acrosome, nucleus and mitochondrial sheath of these spermatozoa appeared normal, while the flagellum displayed structural abnormalities including deformation of the two longitudinal columns of the FS and disruption of a portion of FS, suggesting that Vad1.2 might be involved in the biogenesis of FS in spermatogenesis. Furthermore, Vad1.2 interacted with Akap4 in vivo, and the two proteins were co-immunoprecipitated from the testis or cauda epididymal spermatozoa lysates. Akap4 and Vad1.2 were localized to the tail region of the testicular spermatids and cauda epididymal spermatozoa. The expression levels of pro- and mature Akap4 in Vad1.2-/- testes were markedly increased when compared with the wild-type mice. However, a significant decrease of Akap4 was found in the mutant cauda epididymal spermatozoa, suggesting that most of the mature Akap4 failed to incorporate into the FS. Taken together, Vad1.2 plays… Advisors/Committee Members: Yeung, WSB, Lee, CKF.

Subjects/Keywords: Gene targeting; Spermatogenesis in animals - Genetic aspects

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APA (6^th Edition):

Cao, S. (2012). Gene targeting to study a novel testis-specific gene Vad1.2 in spermatogenesis. (Doctoral Dissertation). University of Hong Kong. Retrieved from Cao, S. [曹善柏]. (2012). Gene targeting to study a novel testis-specific gene Vad1.2 in spermatogenesis. (Thesis). University of Hong Kong, Pokfulam, Hong Kong SAR. Retrieved from http://dx.doi.org/10.5353/th_b4979925 ; http://dx.doi.org/10.5353/th_b4979925 ; http://hdl.handle.net/10722/207896

Chicago Manual of Style (16^th Edition):

Cao, Shanbo. “Gene targeting to study a novel testis-specific gene Vad1.2 in spermatogenesis.” 2012. Doctoral Dissertation, University of Hong Kong. Accessed December 15, 2019. Cao, S. [曹善柏]. (2012). Gene targeting to study a novel testis-specific gene Vad1.2 in spermatogenesis. (Thesis). University of Hong Kong, Pokfulam, Hong Kong SAR. Retrieved from http://dx.doi.org/10.5353/th_b4979925 ; http://dx.doi.org/10.5353/th_b4979925 ; http://hdl.handle.net/10722/207896.

MLA Handbook (7^th Edition):

Cao, Shanbo. “Gene targeting to study a novel testis-specific gene Vad1.2 in spermatogenesis.” 2012. Web. 15 Dec 2019.

Vancouver:

Cao S. Gene targeting to study a novel testis-specific gene Vad1.2 in spermatogenesis. [Internet] [Doctoral dissertation]. University of Hong Kong; 2012. [cited 2019 Dec 15]. Available from: Cao, S. [曹善柏]. (2012). Gene targeting to study a novel testis-specific gene Vad1.2 in spermatogenesis. (Thesis). University of Hong Kong, Pokfulam, Hong Kong SAR. Retrieved from http://dx.doi.org/10.5353/th_b4979925 ; http://dx.doi.org/10.5353/th_b4979925 ; http://hdl.handle.net/10722/207896.

Council of Science Editors:

Cao S. Gene targeting to study a novel testis-specific gene Vad1.2 in spermatogenesis. [Doctoral Dissertation]. University of Hong Kong; 2012. Available from: Cao, S. [曹善柏]. (2012). Gene targeting to study a novel testis-specific gene Vad1.2 in spermatogenesis. (Thesis). University of Hong Kong, Pokfulam, Hong Kong SAR. Retrieved from http://dx.doi.org/10.5353/th_b4979925 ; http://dx.doi.org/10.5353/th_b4979925 ; http://hdl.handle.net/10722/207896

University of Hong Kong

25. Zuo, Yan. Gene targeting to study a novel acrosome specific gene VAD1.3/AEP1 in spermatogenesis.

Degree: PhD, 2008, University of Hong Kong

URL: Zuo, Y. [左妍]. (2008). Gene targeting to study a novel acrosome specific gene VAD1.3/AEP1 in spermatogenesis. (Thesis). University of Hong Kong, Pokfulam, Hong Kong SAR. Retrieved from http://dx.doi.org/10.5353/th_b4163425 ; http://dx.doi.org/10.5353/th_b4163425 ; http://hdl.handle.net/10722/54422

published_or_final_version

Obstetrics and Gynaecology

Doctoral

Doctor of Philosophy

Advisors/Committee Members: Lee, CKF, Yeung, WSB.

Subjects/Keywords: Spermatogenesis.; Gene targeting.

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APA (6^th Edition):

Zuo, Y. (2008). Gene targeting to study a novel acrosome specific gene VAD1.3/AEP1 in spermatogenesis. (Doctoral Dissertation). University of Hong Kong. Retrieved from Zuo, Y. [左妍]. (2008). Gene targeting to study a novel acrosome specific gene VAD1.3/AEP1 in spermatogenesis. (Thesis). University of Hong Kong, Pokfulam, Hong Kong SAR. Retrieved from http://dx.doi.org/10.5353/th_b4163425 ; http://dx.doi.org/10.5353/th_b4163425 ; http://hdl.handle.net/10722/54422

Chicago Manual of Style (16^th Edition):

Zuo, Yan. “Gene targeting to study a novel acrosome specific gene VAD1.3/AEP1 in spermatogenesis.” 2008. Doctoral Dissertation, University of Hong Kong. Accessed December 15, 2019. Zuo, Y. [左妍]. (2008). Gene targeting to study a novel acrosome specific gene VAD1.3/AEP1 in spermatogenesis. (Thesis). University of Hong Kong, Pokfulam, Hong Kong SAR. Retrieved from http://dx.doi.org/10.5353/th_b4163425 ; http://dx.doi.org/10.5353/th_b4163425 ; http://hdl.handle.net/10722/54422.

MLA Handbook (7^th Edition):

Zuo, Yan. “Gene targeting to study a novel acrosome specific gene VAD1.3/AEP1 in spermatogenesis.” 2008. Web. 15 Dec 2019.

Vancouver:

Zuo Y. Gene targeting to study a novel acrosome specific gene VAD1.3/AEP1 in spermatogenesis. [Internet] [Doctoral dissertation]. University of Hong Kong; 2008. [cited 2019 Dec 15]. Available from: Zuo, Y. [左妍]. (2008). Gene targeting to study a novel acrosome specific gene VAD1.3/AEP1 in spermatogenesis. (Thesis). University of Hong Kong, Pokfulam, Hong Kong SAR. Retrieved from http://dx.doi.org/10.5353/th_b4163425 ; http://dx.doi.org/10.5353/th_b4163425 ; http://hdl.handle.net/10722/54422.

Council of Science Editors:

Zuo Y. Gene targeting to study a novel acrosome specific gene VAD1.3/AEP1 in spermatogenesis. [Doctoral Dissertation]. University of Hong Kong; 2008. Available from: Zuo, Y. [左妍]. (2008). Gene targeting to study a novel acrosome specific gene VAD1.3/AEP1 in spermatogenesis. (Thesis). University of Hong Kong, Pokfulam, Hong Kong SAR. Retrieved from http://dx.doi.org/10.5353/th_b4163425 ; http://dx.doi.org/10.5353/th_b4163425 ; http://hdl.handle.net/10722/54422

University of Hong Kong

26. 李穎雯.; Li, Wing-man, Michelle. Gap junction regulates the blood-testis barrier dynamics during spermatogenesis.

Degree: PhD, 2010, University of Hong Kong

URL: Li, W. M. [李穎雯]. (2010). Gap junction regulates the blood-testis barrier dynamics during spermatogenesis. (Thesis). University of Hong Kong, Pokfulam, Hong Kong SAR. Retrieved from http://dx.doi.org/10.5353/th_b4545022 ; http://dx.doi.org/10.5353/th_b4545022 ; http://hdl.handle.net/10722/132274

published_or_final_version

Biological Sciences

Doctoral

Doctor of Philosophy

Advisors/Committee Members: Lee, WWM.

Subjects/Keywords: Spermatogenesis.; Cell junctions.

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APA (6^th Edition):

李穎雯.; Li, Wing-man, M. (2010). Gap junction regulates the blood-testis barrier dynamics during spermatogenesis. (Doctoral Dissertation). University of Hong Kong. Retrieved from Li, W. M. [李穎雯]. (2010). Gap junction regulates the blood-testis barrier dynamics during spermatogenesis. (Thesis). University of Hong Kong, Pokfulam, Hong Kong SAR. Retrieved from http://dx.doi.org/10.5353/th_b4545022 ; http://dx.doi.org/10.5353/th_b4545022 ; http://hdl.handle.net/10722/132274

Chicago Manual of Style (16^th Edition):

李穎雯.; Li, Wing-man, Michelle. “Gap junction regulates the blood-testis barrier dynamics during spermatogenesis.” 2010. Doctoral Dissertation, University of Hong Kong. Accessed December 15, 2019. Li, W. M. [李穎雯]. (2010). Gap junction regulates the blood-testis barrier dynamics during spermatogenesis. (Thesis). University of Hong Kong, Pokfulam, Hong Kong SAR. Retrieved from http://dx.doi.org/10.5353/th_b4545022 ; http://dx.doi.org/10.5353/th_b4545022 ; http://hdl.handle.net/10722/132274.

MLA Handbook (7^th Edition):

李穎雯.; Li, Wing-man, Michelle. “Gap junction regulates the blood-testis barrier dynamics during spermatogenesis.” 2010. Web. 15 Dec 2019.

Vancouver:

李穎雯.; Li, Wing-man M. Gap junction regulates the blood-testis barrier dynamics during spermatogenesis. [Internet] [Doctoral dissertation]. University of Hong Kong; 2010. [cited 2019 Dec 15]. Available from: Li, W. M. [李穎雯]. (2010). Gap junction regulates the blood-testis barrier dynamics during spermatogenesis. (Thesis). University of Hong Kong, Pokfulam, Hong Kong SAR. Retrieved from http://dx.doi.org/10.5353/th_b4545022 ; http://dx.doi.org/10.5353/th_b4545022 ; http://hdl.handle.net/10722/132274.

Council of Science Editors:

李穎雯.; Li, Wing-man M. Gap junction regulates the blood-testis barrier dynamics during spermatogenesis. [Doctoral Dissertation]. University of Hong Kong; 2010. Available from: Li, W. M. [李穎雯]. (2010). Gap junction regulates the blood-testis barrier dynamics during spermatogenesis. (Thesis). University of Hong Kong, Pokfulam, Hong Kong SAR. Retrieved from http://dx.doi.org/10.5353/th_b4545022 ; http://dx.doi.org/10.5353/th_b4545022 ; http://hdl.handle.net/10722/132274

Kent State University

27. Joudeh, Nidaa M. THE ROLE OF SERINE/THREONINE PHOSPHATASES IN SPERMFUNCTION.

Degree: PhD, College of Arts and Sciences / Department of Biological Sciences, 2017, Kent State University

URL: http://rave.ohiolink.edu/etdc/view?acc_num=kent1493594290482411

► This thesis sheds light on the role of two main Ser/Thr phosphatases in sperm function:PP1¿2, and Calcineurin (PP2B). The results show for the first time… (more)

▼ This thesis sheds light on the role of two main Ser/Thr phosphatases in sperm function:PP1¿2, and Calcineurin (PP2B). The results show for the first time that spermatogenesis in thetestis can be supported by the somatic PP1 isoform PP1¿1, and that the unique male germ cellphosphatase “PP1¿2” has probably evolved in mammals along with the evolution of themammalian epididymis for the sole purpose of supporting sperm epididymal maturation.We suggest that calcineurin has high activity in caput sperm due to the high levels ofintracellular calcium. And that the effect of high calcium levels along with high phosphataseactivity in caput sperm, have an inhibitory effect on sperm motility, opposing the effect ofcalcium on motility during sperm hyperactivation in the female reproductive tract. We proposethat calcium signaling in sperm function has a biphasic role depending on which calciumdependent proteins are selectively activated at each developmental stage of the sperm journey.Calcieurin may also have a role in regulating mitochondrial activity. A shift in energy productionpathways and substrate utilization occurs during sperm transition from the caput to the caudaepididymis, and calcineurin is believed to play a role in this process.With PP1¿2 shown to be irreplaceable by PP1¿1 during sperm epididymal maturation, thefollowing step would be comparing the phosphoproteome of PP1¿2 bearing sperm with those ofPP1¿1 bearing sperm. A wide phosphoproteome analysis will reveal the specific substrates ofPP1¿2 that PP1¿1 are unable to dephosphorylate, highlighting proteins with an essential role inepididymal maturation and sperm motility regulation. There is a possibility that the phenotype ofPP1¿1 rescue males is caused by the effect of the transgenes incorporation into a random region145of the mouse genome. The only way to test this hypothesis would be the generation of a newtransgenic model using a gene knock-in technique, where the endogenous promoters functionwill be maintained, and there will be no random incorporation of genetic material into thegenome. If the results of that new transgenic model support our data, then only we can confirmour conclusion.There is strong evidence that calcineurin has a role in mitochondrial energy productionand activity. The following step would be analyzing PPP3R2 KO males for additionalmitochondrial function parameters, such as oxygen consumption or measurement of a number ofmitochondrial enzyme activities. Scanning electron microscopy can be performed to look for anymitochondrial elongation or fusion. Advisors/Committee Members: Vijayaraghavan, Srinivasan (Advisor).

Subjects/Keywords: Biology; Spermatogenesis; Sperm function; Male reproduction

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APA (6^th Edition):

Joudeh, N. M. (2017). THE ROLE OF SERINE/THREONINE PHOSPHATASES IN SPERMFUNCTION. (Doctoral Dissertation). Kent State University. Retrieved from http://rave.ohiolink.edu/etdc/view?acc_num=kent1493594290482411

Chicago Manual of Style (16^th Edition):

Joudeh, Nidaa M. “THE ROLE OF SERINE/THREONINE PHOSPHATASES IN SPERMFUNCTION.” 2017. Doctoral Dissertation, Kent State University. Accessed December 15, 2019. http://rave.ohiolink.edu/etdc/view?acc_num=kent1493594290482411.

MLA Handbook (7^th Edition):

Joudeh, Nidaa M. “THE ROLE OF SERINE/THREONINE PHOSPHATASES IN SPERMFUNCTION.” 2017. Web. 15 Dec 2019.

Vancouver:

Joudeh NM. THE ROLE OF SERINE/THREONINE PHOSPHATASES IN SPERMFUNCTION. [Internet] [Doctoral dissertation]. Kent State University; 2017. [cited 2019 Dec 15]. Available from: http://rave.ohiolink.edu/etdc/view?acc_num=kent1493594290482411.

Council of Science Editors:

Joudeh NM. THE ROLE OF SERINE/THREONINE PHOSPHATASES IN SPERMFUNCTION. [Doctoral Dissertation]. Kent State University; 2017. Available from: http://rave.ohiolink.edu/etdc/view?acc_num=kent1493594290482411

McGill University

28. Dempster, Alastair Robert. Maintenance of the Testis of Acheta Domesticus (L.) In Vitro.

Degree: PhD, Department of Entomology, 1984, McGill University

URL: http://digitool.library.mcgill.ca/thesisfile154079.pdf

►

The in vitro maintenance of the testis of Acheta domesticus (Orthoptera) was attempted. Oxygen consumption measurements of incubated testes showed that they could be maintained… (more)

Subjects/Keywords: Crickets.; Spermatogenesis.; Testis.

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APA (6^th Edition):

Dempster, A. R. (1984). Maintenance of the Testis of Acheta Domesticus (L.) In Vitro. (Doctoral Dissertation). McGill University. Retrieved from http://digitool.library.mcgill.ca/thesisfile154079.pdf

Chicago Manual of Style (16^th Edition):

Dempster, Alastair Robert. “Maintenance of the Testis of Acheta Domesticus (L.) In Vitro.” 1984. Doctoral Dissertation, McGill University. Accessed December 15, 2019. http://digitool.library.mcgill.ca/thesisfile154079.pdf.

MLA Handbook (7^th Edition):

Dempster, Alastair Robert. “Maintenance of the Testis of Acheta Domesticus (L.) In Vitro.” 1984. Web. 15 Dec 2019.

Vancouver:

Dempster AR. Maintenance of the Testis of Acheta Domesticus (L.) In Vitro. [Internet] [Doctoral dissertation]. McGill University; 1984. [cited 2019 Dec 15]. Available from: http://digitool.library.mcgill.ca/thesisfile154079.pdf.

Council of Science Editors:

Dempster AR. Maintenance of the Testis of Acheta Domesticus (L.) In Vitro. [Doctoral Dissertation]. McGill University; 1984. Available from: http://digitool.library.mcgill.ca/thesisfile154079.pdf

East Carolina University

29. Kirsanov, Oleksandr. ANALYSIS OF TRANSGENIC MOUSE MODELS TO STUDY MAMMALIAN SPERMATOGONIA.

Degree: 2018, East Carolina University

URL: http://hdl.handle.net/10342/6969

► Spermatogenesis in the mammalian testis results in the daily production of millions of spermatozoa. This developmental process is founded upon the actions of a small… (more)

Subjects/Keywords: Spermatogonia; Animals; Mice, Transgenic; Mammals; Spermatogenesis

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APA (6^th Edition):

Kirsanov, O. (2018). ANALYSIS OF TRANSGENIC MOUSE MODELS TO STUDY MAMMALIAN SPERMATOGONIA. (Thesis). East Carolina University. Retrieved from http://hdl.handle.net/10342/6969

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Kirsanov, Oleksandr. “ANALYSIS OF TRANSGENIC MOUSE MODELS TO STUDY MAMMALIAN SPERMATOGONIA.” 2018. Thesis, East Carolina University. Accessed December 15, 2019. http://hdl.handle.net/10342/6969.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Kirsanov, Oleksandr. “ANALYSIS OF TRANSGENIC MOUSE MODELS TO STUDY MAMMALIAN SPERMATOGONIA.” 2018. Web. 15 Dec 2019.

Vancouver:

Kirsanov O. ANALYSIS OF TRANSGENIC MOUSE MODELS TO STUDY MAMMALIAN SPERMATOGONIA. [Internet] [Thesis]. East Carolina University; 2018. [cited 2019 Dec 15]. Available from: http://hdl.handle.net/10342/6969.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Kirsanov O. ANALYSIS OF TRANSGENIC MOUSE MODELS TO STUDY MAMMALIAN SPERMATOGONIA. [Thesis]. East Carolina University; 2018. Available from: http://hdl.handle.net/10342/6969

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Cornell University

30. Hilz, Stephanie Renee. Elucidating Roles For Ago-Associated Small Rnas In Male Mammalian Meiosis Using Integrative Transcriptome Profiling.

Degree: 2016, Cornell University

URL: http://hdl.handle.net/1813/45350

Subjects/Keywords: microRNAs; spermatogenesis; meiosis

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APA (6^th Edition):

Hilz, S. R. (2016). Elucidating Roles For Ago-Associated Small Rnas In Male Mammalian Meiosis Using Integrative Transcriptome Profiling. (Thesis). Cornell University. Retrieved from http://hdl.handle.net/1813/45350

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Hilz, Stephanie Renee. “Elucidating Roles For Ago-Associated Small Rnas In Male Mammalian Meiosis Using Integrative Transcriptome Profiling. ” 2016. Thesis, Cornell University. Accessed December 15, 2019. http://hdl.handle.net/1813/45350.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Hilz, Stephanie Renee. “Elucidating Roles For Ago-Associated Small Rnas In Male Mammalian Meiosis Using Integrative Transcriptome Profiling. ” 2016. Web. 15 Dec 2019.

Vancouver:

Hilz SR. Elucidating Roles For Ago-Associated Small Rnas In Male Mammalian Meiosis Using Integrative Transcriptome Profiling. [Internet] [Thesis]. Cornell University; 2016. [cited 2019 Dec 15]. Available from: http://hdl.handle.net/1813/45350.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Hilz SR. Elucidating Roles For Ago-Associated Small Rnas In Male Mammalian Meiosis Using Integrative Transcriptome Profiling. [Thesis]. Cornell University; 2016. Available from: http://hdl.handle.net/1813/45350

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Open Access Theses and Dissertations