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You searched for subject:(sexed sperm). Showing records 1 – 3 of 3 total matches.

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Colorado State University

1. Rasmussen, Sara-Lesley. Applying in vitro-produced embryos and sexed sperm to dairy cattle reproduction.

Degree: MS(M.S.), Biomedical Sciences, 2011, Colorado State University

This study compared the pregnancy rates between embryo transfer of bovine embryos produced in vitro with sexed vs control sperm and artificial insemination (AI) using sexed and unsexed sperm. Cleavage rates for oocytes fertilized with sexed vs control sperm were not different for two of the three bulls used, but were lower (p < 0.05) for the third bull sexed (44%) vs control sperm (70%). There were fewer transferable blastocysts produced per oocyte with sexed sperm (9-19%) than for unsexed sperm (18-26%); (p < 0.05). All cows were on an Ovsynch program to synchronize ovulation. Respective 60 d pregnancy rates at two Colorado dairies were as follows: control AI (43%, n=88; 43%, n=44); AI with X-sorted sperm (34%, n=82; 34%, n=62); embryo transfer (ET) with in vitro-produced (IVP) embryos using unsexed sperm (22%, n=68; 21%, n=39); and ET with IVP embryos using sexed sperm (7%, n=72; 37%, n=40). The pregnancy rate (day 60) for AI using sexed sperm was 78% of that of control sperm. ET pregnancy rates were generally lower than AI rates. At one dairy, abortions between days 32 and term were higher for X-sort ET pregnancies (79% n=14) than for AI control pregnancies (20% n=40); (P < 0.001). However, the other dairy experienced only a 12%, (n=17) abortion rate for transferred embryos produced from X-sorted sperm. The sex ratio of calves was similar to previous studies for AI with control sperm (52% bull calves, n=50), AI with X-sorted sperm (12% bull calves, n=40); ET with IVP embryos using unsexed sperm (50% bull calves, n=18); and ET with IVP embryos using sexed sperm (11% bull calves, n=18). Findings from this experiment indicate that embryo production with sexed sperm is not successful enough to be applied to large-scale dairies that already have successful breeding programs in place. Advisors/Committee Members: Seidel, George E., Jr. (advisor), Graham, James K. (committee member), McCue, Patrick M. (committee member).

Subjects/Keywords: assisted reproduction technologies; dairy cattle fertility; embryo transfer; IVF; pregnancy rates; sexed sperm

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6th Edition):

Rasmussen, S. (2011). Applying in vitro-produced embryos and sexed sperm to dairy cattle reproduction. (Masters Thesis). Colorado State University. Retrieved from http://hdl.handle.net/10217/47443

Chicago Manual of Style (16th Edition):

Rasmussen, Sara-Lesley. “Applying in vitro-produced embryos and sexed sperm to dairy cattle reproduction.” 2011. Masters Thesis, Colorado State University. Accessed April 12, 2021. http://hdl.handle.net/10217/47443.

MLA Handbook (7th Edition):

Rasmussen, Sara-Lesley. “Applying in vitro-produced embryos and sexed sperm to dairy cattle reproduction.” 2011. Web. 12 Apr 2021.

Vancouver:

Rasmussen S. Applying in vitro-produced embryos and sexed sperm to dairy cattle reproduction. [Internet] [Masters thesis]. Colorado State University; 2011. [cited 2021 Apr 12]. Available from: http://hdl.handle.net/10217/47443.

Council of Science Editors:

Rasmussen S. Applying in vitro-produced embryos and sexed sperm to dairy cattle reproduction. [Masters Thesis]. Colorado State University; 2011. Available from: http://hdl.handle.net/10217/47443


Lincoln University

2. Regtien, D. R. The potential of gender-sorted semen for improving dairy farm profitability, efficiency and genetic gain in New Zealand : A dissertation submitted in partial fulfillment of the requirement for the Degree of BAgrSc (Honours).

Degree: 2012, Lincoln University

Contents of CD to be added. Pagination discrepancy - vii, 8-69, 69 pages in total

Gender sorted semen, or "sexed-semen" is a new biotechnology available in New Zealand. There are potential benefits associated with its use on dairy farms, including a greater number of heifer calves born allowing for increased genetic selection pressure for herd replacements, increased income from sale of surplus heifer stock, improvements in animal welfare and improvements in the efficiency of milk production. The disadvantages of the technology include the reduced conception rate and final calving rate of cows, as well as the increased insemination costs and lower average genetic merit of sires available. A bio-economic model is presented that enables comparison of four sexed-semen scenarios in five simulated herds that were based on animals from the Lincoln University Dairy Farm. This enabled analysis of the costs and benefits resulting from the use of sexed­ semen, as well as variation between herds and sensitivities. The marginal net present value outcomes were; -284 for a frozen sexed-semen scenario, 1,056 for a fresh sexed-semen scenario, 353 for the use of sexed-semen sorted to 75% purity and 5,096 for a scenario based on estimates of parameters possible with an improved technology. The results indicate the unreliability of net financial gain through using current gender-sorted semen technology, especially with the use of frozen sexed­ semen. The non-financial benefits and limitations of the products are discussed, and is was concluded that sexed-semen is a breeding technology that allows more flexibility to the management of dairy farms, especially if problems due to sperm viability and sperm wastage during processing can be resolved. If some of the challenges can be addressed, the benefits for milk producers and the dairy industry are substantial.

Subjects/Keywords: sexed-semen; dairy; biotechnology; flow cytometry; sperm sorting; reproduction; genetics; economics; 0701 Agriculture, Land and Farm Management; 070206 Animal Reproduction; 080110 Simulation and Modelling

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6th Edition):

Regtien, D. R. (2012). The potential of gender-sorted semen for improving dairy farm profitability, efficiency and genetic gain in New Zealand : A dissertation submitted in partial fulfillment of the requirement for the Degree of BAgrSc (Honours). (Thesis). Lincoln University. Retrieved from http://hdl.handle.net/10182/13118

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16th Edition):

Regtien, D R. “The potential of gender-sorted semen for improving dairy farm profitability, efficiency and genetic gain in New Zealand : A dissertation submitted in partial fulfillment of the requirement for the Degree of BAgrSc (Honours).” 2012. Thesis, Lincoln University. Accessed April 12, 2021. http://hdl.handle.net/10182/13118.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7th Edition):

Regtien, D R. “The potential of gender-sorted semen for improving dairy farm profitability, efficiency and genetic gain in New Zealand : A dissertation submitted in partial fulfillment of the requirement for the Degree of BAgrSc (Honours).” 2012. Web. 12 Apr 2021.

Vancouver:

Regtien DR. The potential of gender-sorted semen for improving dairy farm profitability, efficiency and genetic gain in New Zealand : A dissertation submitted in partial fulfillment of the requirement for the Degree of BAgrSc (Honours). [Internet] [Thesis]. Lincoln University; 2012. [cited 2021 Apr 12]. Available from: http://hdl.handle.net/10182/13118.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Regtien DR. The potential of gender-sorted semen for improving dairy farm profitability, efficiency and genetic gain in New Zealand : A dissertation submitted in partial fulfillment of the requirement for the Degree of BAgrSc (Honours). [Thesis]. Lincoln University; 2012. Available from: http://hdl.handle.net/10182/13118

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

3. Andrés Mejia Gallego. Avaliação das características da motilidade (CASA), morfologia e funcionalidade da membrana plasmática (HOST) de espermatozóides bovinos sexados por cimetria de fluxo.

Degree: 2010, University of São Paulo

A pré-seleção do sexo tem grande impacto na produtividade da bovinocultura de corte e leite. A única técnica comercialmente viável para a pré-seleção do sexo, até agora, é a sexagem espermática utilizando citometria de fluxo. Atualmente palhetas de sêmen com espermatozóides sexados vêm sendo utilizadas regularmente em programas de inseminação artificial. Entretanto, ainda existem limitações referentes à taxa de gestação em condições de campo, explicadas em parte pela perda da integridade celular dos espermatozóides. O intuito deste experimento foi avaliar os efeitos da sexagem e o tempo de espera do ejaculado para a sexagem as 0, 3 e 6 hs sobre os parâmetros de motilidade pelo sistema computadorizado da motilidade espermática (CASA), funcionalidade da membrana plasmática pelo teste de resistência osmótica (HOST), morfologia dos espermatozóides pela técnica de contraste de interferência diferencial (DIC) e entre as subespécies taurinas (Bos taurus taurus) e zebuínas (Bos taurus indicus). Espermatozóides sexados apresentaram a maioria dos parâmetros de motilidade superiores aos submetidos à técnica convencional, indicando que existe uma seleção por parte do citômetro de fluxo. O tempo em que o espermatozóide bovino espera (até seis horas) para ser submetido ao processo de sexagem pela técnica de citometria de fluxo alterou a linearidade (LIN). A funcionalidade da membrana plasmática foi afetada quando o espermatozóide bovino passa pelo processo de sexagem.. A sexagem induz o aumento de defeitos menores, provavelmente por alterações da membrana plasmática da cauda dos espermatozóides. Os zebuínos são mais sensíveis às alterações da membrana plasmática da cauda em relação aos taurinos. Finalmente existem fortes indícios que espermatozóides bovinos que passam pela técnica de citometria de fluxo entrem em estado de hiperativação.

Sex selecting has a huge impact in dairy and beef production. The only way commercially viable to obtain sex selecting nowadays, is a flow citometry sex sorted sperm technique. Currently sex sorted sperm have been used regularly in Artificial Insemination programs. Meanwhile, there are limitations about non return rate in farm conditions, superficially explained as cellular loss integrity. The objective of this experiment was assessing sex sorted sperm effects and time to wait 0, 3 and 6 hours before sorting in motility parameters by computer assisted sperm analysis (CASA), plasmatic membrane functionality by hiposmotic swelling test (HOST), sperm morphology by differential interference contrast microcopy (DIC) and between taurine subspecies (Bos taurus taurus) zebuine subspecies (Bos taurus indicus). Sex sorted sperm showed best results in the most motility parameters compared with conventional sperm, suggesting a flow citometer selection. The time to wait of the sperm (untis 6 hours) to keep sorting, altered a linearity (LIN). Plasmatic membrane functionality was affected when sperm runs through flow citometer.. Sex sorted induces increase of minor defects in sperm, probably by tail…

Advisors/Committee Members: Rubens Paes de Arruda, André Furugen Cesar de Andrade, Lúcia Helena Rodrigues.

Subjects/Keywords: Bovinos; Patologia espermática; Sêmen sexado; Sistema computadorizado de avaliação da motilidade; Teste hiposmotico; Bovine; Computer Assisted Motility Analysis; Hyposmotic swelling test; Sexed semen; Sperm pathology

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6th Edition):

Gallego, A. M. (2010). Avaliação das características da motilidade (CASA), morfologia e funcionalidade da membrana plasmática (HOST) de espermatozóides bovinos sexados por cimetria de fluxo. (Masters Thesis). University of São Paulo. Retrieved from http://www.teses.usp.br/teses/disponiveis/10/10131/tde-01042011-143106/

Chicago Manual of Style (16th Edition):

Gallego, Andrés Mejia. “Avaliação das características da motilidade (CASA), morfologia e funcionalidade da membrana plasmática (HOST) de espermatozóides bovinos sexados por cimetria de fluxo.” 2010. Masters Thesis, University of São Paulo. Accessed April 12, 2021. http://www.teses.usp.br/teses/disponiveis/10/10131/tde-01042011-143106/.

MLA Handbook (7th Edition):

Gallego, Andrés Mejia. “Avaliação das características da motilidade (CASA), morfologia e funcionalidade da membrana plasmática (HOST) de espermatozóides bovinos sexados por cimetria de fluxo.” 2010. Web. 12 Apr 2021.

Vancouver:

Gallego AM. Avaliação das características da motilidade (CASA), morfologia e funcionalidade da membrana plasmática (HOST) de espermatozóides bovinos sexados por cimetria de fluxo. [Internet] [Masters thesis]. University of São Paulo; 2010. [cited 2021 Apr 12]. Available from: http://www.teses.usp.br/teses/disponiveis/10/10131/tde-01042011-143106/.

Council of Science Editors:

Gallego AM. Avaliação das características da motilidade (CASA), morfologia e funcionalidade da membrana plasmática (HOST) de espermatozóides bovinos sexados por cimetria de fluxo. [Masters Thesis]. University of São Paulo; 2010. Available from: http://www.teses.usp.br/teses/disponiveis/10/10131/tde-01042011-143106/

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