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You searched for subject:(sex sorting). Showing records 1 – 3 of 3 total matches.

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Colorado State University

1. Burroughs, Chelsie Ann. Removing seminal plasma improves sex-sorting of bovine sperm.

Degree: MS(M.S.), Biomedical Sciences, 2011, Colorado State University

To view the abstract, please see the full text of the document. Advisors/Committee Members: Seidel, George E., Jr. (advisor), Graham, James K. (advisor), Curthoys, Norman P. (committee member).

Subjects/Keywords: bovine; spermatozoa; sex-sorting; seminal plasma

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6th Edition):

Burroughs, C. A. (2011). Removing seminal plasma improves sex-sorting of bovine sperm. (Masters Thesis). Colorado State University. Retrieved from http://hdl.handle.net/10217/49864

Chicago Manual of Style (16th Edition):

Burroughs, Chelsie Ann. “Removing seminal plasma improves sex-sorting of bovine sperm.” 2011. Masters Thesis, Colorado State University. Accessed April 19, 2021. http://hdl.handle.net/10217/49864.

MLA Handbook (7th Edition):

Burroughs, Chelsie Ann. “Removing seminal plasma improves sex-sorting of bovine sperm.” 2011. Web. 19 Apr 2021.

Vancouver:

Burroughs CA. Removing seminal plasma improves sex-sorting of bovine sperm. [Internet] [Masters thesis]. Colorado State University; 2011. [cited 2021 Apr 19]. Available from: http://hdl.handle.net/10217/49864.

Council of Science Editors:

Burroughs CA. Removing seminal plasma improves sex-sorting of bovine sperm. [Masters Thesis]. Colorado State University; 2011. Available from: http://hdl.handle.net/10217/49864


University of Sydney

2. Bathgate, Roslyn Anne. Studies on the cryopreservation of boar spermatozoa and its integration into assisted reproductive technologies .

Degree: 2004, University of Sydney

The aim of this thesis was to investigate the possibility of integrating frozen-thawed boar semen into reproductive technologies and into commercial production of pigs in Australia. This was to be achieved by establishing a semen freezing and AI regime that was of a standard acceptable to industry, and integrating the resultant frozen-thawed sperm into other reproductive technologies, such as flow cytometric sperm sorting and IVF. Initially, a protocol for freezing and thawing boar semen was established, based on the method described by Westendorf et al. (1975) and attempts were made to modify this protocol to improve the post-thaw sperm quality, as determined by in vitro assessment of motility, acrosome integrity and longevity. First, the egg yolk used in the freezing extenders was investigated, and the chicken yolk was replaced with either duck or quail yolk. It was shown that there was no benefit in substituting yolk from duck or quail for the chicken yolk traditionally used in freezing extender. Second, the effect of seminal plasma addition to the freezing extender, or seminal plasma addition to resuspension medium post-thaw was tested. Incorporating whole seminal plasma into the freezing extender at levels above 50% was found to be detrimental to post-thaw sperm quality. Reducing levels to 20% of the final volume improved acrosome integrity, but adversely affected motility of sperm. However, adding 20% seminal plasma to the resuspension medium used after thawing of boar semen had no significant influence on sperm quality compared with resuspension in medium without seminal plasma. The antioxidant catalase, and the iron chelator desferal added to the freezing extender, did not improve post-thaw sperm quality, nor was any benefit seen with addition of these substrates to the resuspension medium post-thaw. However, the bioactive phospholipid PAF and its regulating enzyme PAF:AH appeared to enhance post-thaw motility and acrosome integrity of sperm, respectively, when added to the semen pre-freezing. Unfortunately, due to the restrictions imposed on rPAF:AH as a research drug, it was not possible to test the in vivo effects at this time. After the in vitro experiments were completed, the in vivo fertility of frozen-thawed sperm was tested using the optimal freezing protocol and a novel technology, enabling non-surgical deep intrauterine insemination of sows. The aim was to establish the lowest possible dose of frozen-thawed sperm that could be used, without compromising fertility. Successful pregnancies were achieved with doses as low as 62.5 x 106 frozen-thawed sperm but the farrowing rates were too low to be practicable on a commercial scale. This is the first report of litters born after insemination of such a low dose of frozen-thawed sperm and using the novel DIU insemination technique. However, it was concluded that a double dose of 250 x 106 frozen-thawed sperm was the minimum dose required for maintaining acceptable fertility. Reduction in sperm numbers to such an extent made it possible…

Subjects/Keywords: pig; cryopreservation; sperm; sex-sorting; AI

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6th Edition):

Bathgate, R. A. (2004). Studies on the cryopreservation of boar spermatozoa and its integration into assisted reproductive technologies . (Thesis). University of Sydney. Retrieved from http://hdl.handle.net/2123/1279

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16th Edition):

Bathgate, Roslyn Anne. “Studies on the cryopreservation of boar spermatozoa and its integration into assisted reproductive technologies .” 2004. Thesis, University of Sydney. Accessed April 19, 2021. http://hdl.handle.net/2123/1279.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7th Edition):

Bathgate, Roslyn Anne. “Studies on the cryopreservation of boar spermatozoa and its integration into assisted reproductive technologies .” 2004. Web. 19 Apr 2021.

Vancouver:

Bathgate RA. Studies on the cryopreservation of boar spermatozoa and its integration into assisted reproductive technologies . [Internet] [Thesis]. University of Sydney; 2004. [cited 2021 Apr 19]. Available from: http://hdl.handle.net/2123/1279.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Bathgate RA. Studies on the cryopreservation of boar spermatozoa and its integration into assisted reproductive technologies . [Thesis]. University of Sydney; 2004. Available from: http://hdl.handle.net/2123/1279

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

3. Hendriksen, Peter. X- and Y-chromosomal gene expression during spermatogenesis, studied in the context of separation of X and Y spermatozoa.

Degree: Department of Urology, 1996, Erasmus University Medical Center

textabstractMankind has been interested in S6X preselection since ancient times. Methods presumed to achieve sex preselection have already been described by Greek philosophers as far as 500 B.C. (reviewed by Betteridge, 1984). Although it took almost 2500 years since then to develop reliable and repeatable methods, sex preselection is now possible and is actually applied in both humans and livestock. It can be achieved either by sexing of early embryos or by separation of X and Y chromosome-bearing spermatozoa (X and Y spermatozoa).

Subjects/Keywords: X-chromosomes; Y-chromosomes; cell sorting; gene expression; sex chromosomes; spermatozoa

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6th Edition):

Hendriksen, P. (1996). X- and Y-chromosomal gene expression during spermatogenesis, studied in the context of separation of X and Y spermatozoa. (Doctoral Dissertation). Erasmus University Medical Center. Retrieved from http://hdl.handle.net/1765/22578

Chicago Manual of Style (16th Edition):

Hendriksen, Peter. “X- and Y-chromosomal gene expression during spermatogenesis, studied in the context of separation of X and Y spermatozoa.” 1996. Doctoral Dissertation, Erasmus University Medical Center. Accessed April 19, 2021. http://hdl.handle.net/1765/22578.

MLA Handbook (7th Edition):

Hendriksen, Peter. “X- and Y-chromosomal gene expression during spermatogenesis, studied in the context of separation of X and Y spermatozoa.” 1996. Web. 19 Apr 2021.

Vancouver:

Hendriksen P. X- and Y-chromosomal gene expression during spermatogenesis, studied in the context of separation of X and Y spermatozoa. [Internet] [Doctoral dissertation]. Erasmus University Medical Center; 1996. [cited 2021 Apr 19]. Available from: http://hdl.handle.net/1765/22578.

Council of Science Editors:

Hendriksen P. X- and Y-chromosomal gene expression during spermatogenesis, studied in the context of separation of X and Y spermatozoa. [Doctoral Dissertation]. Erasmus University Medical Center; 1996. Available from: http://hdl.handle.net/1765/22578

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