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Western Kentucky University

1. Abdulrheem, Ali Jamal. Detection and Quantitation of Tetracycline Antibiotics in Agricultural Swine Wastes.

Degree: MS, Department of Chemistry, 2017, Western Kentucky University

The spread of tetracyclines through agricultural systems is causing the present bacteria to develop antibiotic resistance. The spread of this bacteria, as well as the tetracycline antibiotics in the environment is dangerous because these antibiotics pose health hazards for humans. The overuse of antibiotics, which are added to livestock feed, results in the antibiotics being released into the environment via animal feces. In this research, we have attempted to design an analytical method to isolate antibiotics from agricultural wastes with subsequent detection using liquid chromatography and mass spectrometry (LC-MS). The antibiotics investigated in this study were tetracycline, chlortetracycline, and oxytetracycline. The analytical procedure involves mixing the agricultural samples with an organic solvent, such as methanol, which solubilizes these antibiotics. Next, samples are centrifuged to remove solid particulates. A polymeric weak cation cartridge was used to concentrate and separate the antibiotics from the unwanted organic chemical compounds found in the samples. The antibiotics were released with methanol with small amounts of acid and then detected and quantified using LC-MS and high performance liquid chromatography with a photodiode array detector (HPLC-PDA). Advisors/Committee Members: Dr. Eric Conte (Director), Dr. Stuart Burris, and Dr. John Loughrin.

Subjects/Keywords: liquid chromatography; residual bacteria; solid phase extraction; tetracyclines; Environmental Chemistry; Meat Science; Microbiology

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6th Edition):

Abdulrheem, A. J. (2017). Detection and Quantitation of Tetracycline Antibiotics in Agricultural Swine Wastes. (Masters Thesis). Western Kentucky University. Retrieved from https://digitalcommons.wku.edu/theses/1931

Chicago Manual of Style (16th Edition):

Abdulrheem, Ali Jamal. “Detection and Quantitation of Tetracycline Antibiotics in Agricultural Swine Wastes.” 2017. Masters Thesis, Western Kentucky University. Accessed June 20, 2019. https://digitalcommons.wku.edu/theses/1931.

MLA Handbook (7th Edition):

Abdulrheem, Ali Jamal. “Detection and Quantitation of Tetracycline Antibiotics in Agricultural Swine Wastes.” 2017. Web. 20 Jun 2019.

Vancouver:

Abdulrheem AJ. Detection and Quantitation of Tetracycline Antibiotics in Agricultural Swine Wastes. [Internet] [Masters thesis]. Western Kentucky University; 2017. [cited 2019 Jun 20]. Available from: https://digitalcommons.wku.edu/theses/1931.

Council of Science Editors:

Abdulrheem AJ. Detection and Quantitation of Tetracycline Antibiotics in Agricultural Swine Wastes. [Masters Thesis]. Western Kentucky University; 2017. Available from: https://digitalcommons.wku.edu/theses/1931

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