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Georgia State University
1.
Snyder, Charles D, Jr.
Fracture: Failure as a Path to Utopia in Queer Art and Life.
Degree: MFA, Art and Design, 2015, Georgia State University
URL: https://scholarworks.gsu.edu/art_design_theses/175
► Reproductive Futurism is the pervasive structuring of politics and society around securing of the future through fealty to a figurative Child. The primary means…
(more)
▼ Reproductive Futurism is the pervasive structuring of politics and society around securing of the future through fealty to a figurative
Child. The primary means of the
Child’s proliferation is the normalizing of hetero-reproduction, which privileges heterosexuality with validity and meaning.
Fracture represents a body of work that illustrates my personal break with these normalizing structures, and through embracing my queer sexuality a refusal of the figurative
Child and the future it represents. The work articulates the role sex played in my
failure to adhere to (liberation from) normative relational structures, and proposes potential new configurations of bodies and filial bonds born from that
failure. The work also represents a material shift in my work from photographic prints to intermedia/installation. The evolution of my process as an artist was informed by my personal transformation; my vision of the world shifted, so too did my material/aesthetic response to that world.
Advisors/Committee Members: Constance Thalken, Nancy Floyd, Susan Richmond.
Subjects/Keywords: queer; heteronormativity; failure; reproductive futurism; photography; conceptual art
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APA (6th Edition):
Snyder, Charles D, J. (2015). Fracture: Failure as a Path to Utopia in Queer Art and Life. (Thesis). Georgia State University. Retrieved from https://scholarworks.gsu.edu/art_design_theses/175
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Snyder, Charles D, Jr. “Fracture: Failure as a Path to Utopia in Queer Art and Life.” 2015. Thesis, Georgia State University. Accessed January 16, 2021.
https://scholarworks.gsu.edu/art_design_theses/175.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Snyder, Charles D, Jr. “Fracture: Failure as a Path to Utopia in Queer Art and Life.” 2015. Web. 16 Jan 2021.
Vancouver:
Snyder, Charles D J. Fracture: Failure as a Path to Utopia in Queer Art and Life. [Internet] [Thesis]. Georgia State University; 2015. [cited 2021 Jan 16].
Available from: https://scholarworks.gsu.edu/art_design_theses/175.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Snyder, Charles D J. Fracture: Failure as a Path to Utopia in Queer Art and Life. [Thesis]. Georgia State University; 2015. Available from: https://scholarworks.gsu.edu/art_design_theses/175
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Universidade do Rio Grande do Sul
2.
Tochetto, Caroline.
Análise do viroma de soro de matrizes suínas com partos normais e com natimortalidade.
Degree: 2017, Universidade do Rio Grande do Sul
URL: http://hdl.handle.net/10183/172155
► Falhas reprodutivas são importante causa de prejuízos econômicos na suinocultura. Elas implicam na diminuição do número de leitões nascidos vivos e aumentam o descarte de…
(more)
▼ Falhas reprodutivas são importante causa de prejuízos econômicos na suinocultura. Elas implicam na diminuição do número de leitões nascidos vivos e aumentam o descarte de animais e as taxas de reposição de matrizes, levando à redução da produtividade do rebanho. Embora a maioria dos casos de natimortalidade sejam associados a fatores não infecciosos, os agentes infecciosos possuem um papel importante e ainda pouco conhecido na etiologia deste quadro. Até o presente, nenhum trabalho foi realizado visando o estudo do conjunto de vírus que possam estar presentes em matrizes com eventos de natimortalidade por ocasião do parto. Em função disso, o presente trabalho teve por objetivo examinar o viroma do soro de matrizes suínas com e sem casos de natimortalidade. Foram coletadas 94 amostras de soro de matrizes de seis granjas distribuídas em cinco municípios do Rio Grande do Sul. Em cada granja foram formados dois pools de soros: um composto por matrizes que pariram (um ou mais) natimortos e outro por matrizes que pariram leitegadas sem natimortos. Os pools foram submetidos à extração de ácido nucleico viral, enriquecimento e sequenciamento de alto desempenho, buscando a identificação de agentes que possam representar um fator de risco à natimortalidade em suínos Não foi possível identificar diferenças significativas nos viromas de matrizes correlacionadas à ocorrência de natimortalidade. Não obstante, foi possível identificar uma ampla variedade de genomas virais, a maioria deles correspondendo a vírus das famílias Anelloviridae. Este estudo permitiu ainda identificar 20 genomas completos de três espécies de vírus: torque teno vírus suíno 1a e 1b, circovírus suíno tipo 3 (PCV3) e vírus circulares DNA fita simples codificantes de replicase (CRESS), seis dos quais até o presente ainda não reportados em suínos. Em duas granjas, em matrizes que apresentaram natimortalidade, foram identificados genomas de PCV3, cuja participação como potencial causador de problemas reprodutivos precisa ser futuramente investigada. Não foram identificados vírus com genoma de RNA. Este estudo traz uma contribuição ao conhecimento do viroma em soros de matrizes suínas e, paralelamente, busca contribuir para o esclarecimento das possíveis causas de natimortalidade de origem infecciosa em suínos.
Reproductive failure in swine herds is an important cause of economic losses. It leads to a decrease in the number of piglets reared per sow and may imply in the need for replacement of sows, reducing the productivity in a herd. Although the majority of cases of stillbirths have been attributed to non-infectious causes, several infectious agents have been implicated in the etiology of such condition. Nevertheless, other as yet unknown agents may be involved in the pathogenesis of stillbirths. The aim of this work was to investigate the virome in sera of sows without and with one or more cases of stillbirth in the litter. Sera were collected from 94 sows of six commercial farms in five municipalities in the state of Rio Grande do Sul, Brazil. Two pools of…
Advisors/Committee Members: Roehe, Paulo Michel.
Subjects/Keywords: Natimortalidade : Suinos; Pig; Reproductive failure; Sequência genômica; Reprodução animal; Metagenomic; Genoma viral; NGS; Anellovirus
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❌
APA ·
Chicago ·
MLA ·
Vancouver ·
CSE |
Export
to Zotero / EndNote / Reference
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APA (6th Edition):
Tochetto, C. (2017). Análise do viroma de soro de matrizes suínas com partos normais e com natimortalidade. (Thesis). Universidade do Rio Grande do Sul. Retrieved from http://hdl.handle.net/10183/172155
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Tochetto, Caroline. “Análise do viroma de soro de matrizes suínas com partos normais e com natimortalidade.” 2017. Thesis, Universidade do Rio Grande do Sul. Accessed January 16, 2021.
http://hdl.handle.net/10183/172155.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Tochetto, Caroline. “Análise do viroma de soro de matrizes suínas com partos normais e com natimortalidade.” 2017. Web. 16 Jan 2021.
Vancouver:
Tochetto C. Análise do viroma de soro de matrizes suínas com partos normais e com natimortalidade. [Internet] [Thesis]. Universidade do Rio Grande do Sul; 2017. [cited 2021 Jan 16].
Available from: http://hdl.handle.net/10183/172155.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Tochetto C. Análise do viroma de soro de matrizes suínas com partos normais e com natimortalidade. [Thesis]. Universidade do Rio Grande do Sul; 2017. Available from: http://hdl.handle.net/10183/172155
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
3.
Giseli Aparecida Ritterbusch.
Pathogenicity study and co-infection investigation by Porcine Circovirus and Swine Torque Teno Virus in materials from sows with reproductive failure.
Degree: 2009, Universidade do Estado de Santa Catarina
URL: http://www.tede.udesc.br/tde_busca/arquivo.php?codArquivo=3363
► Muitos agentes infecciosos tÃm sido associados Ãs falhas reprodutivas na produÃÃo de suÃnos, representando significativas perdas econÃmicas para os suinocultores. Recentemente o Circovirus SuÃno tipo…
(more)
▼ Muitos agentes infecciosos tÃm sido associados Ãs falhas reprodutivas na produÃÃo de suÃnos, representando significativas perdas econÃmicas para os suinocultores. Recentemente o Circovirus SuÃno tipo 2 (PCV2), agente etiolÃgico da circovirose suÃna, foi associado a falhas reprodutivas em suÃnos em diversas partes do mundo. Para confirmar o potencial patogÃnico do PCV2 causando falhas reprodutivas em porcas, à necessÃrio o isolamento do vÃrus e demonstraÃÃo de antÃgeno e Ãcido nuclÃico viral em fetos. Outro agente viral, o Torque Teno VÃrus (TTV), tambÃm foi recentemente associado Ãs infecÃÃes causadas pelo PCV2. O TTV sozinho ainda nÃo tem se mostrado patogÃnico em suÃnos, porÃm, seu papel em co-infecÃÃes com outros patÃgenos vem sendo investigado. O presente trabalho teve por objetivos diagnosticar o PCV2 em infecÃÃes naturais onde existiam falhas reprodutivas, assim como padronizar e aplicar a tÃcnica de ReaÃÃo em Cadeia da Polimerase (PCR) para TTV a partir de ÃrgÃos. Amostras provenientes de casos clÃnicos de campo, como fetos abortados, mumificados, natimortos, leitÃes inviÃveis e material de fÃmeas descartadas foram coletadas e processadas para diagnÃstico da infecÃÃo pelo PCV2 atravÃs de PCR e imunoistoquÃmica (IHQ). Foram colhidas amostras de 21 granjas produtoras de suÃnos, totalizando 169 fetos, que foram necropsiados para coleta de ÃrgÃos. AlÃm disso, amostras de ÃrgÃos reprodutivos de 83 fÃmeas descartadas foram colhidas em 4 abatedouros da regiÃo oeste catarinense. No presente estudo foi possÃvel detectar DNA viral por PCR em 29 (17,1%) dos 169 fetos analisados, sendo coraÃÃo e tecidos linfÃides os ÃrgÃos onde o vÃrus foi identificado com maior freqÃÃncia, 41,4% e 37,8%, respectivamente. A presenÃa do vÃrus foi confirmada por teste de IHQ dos tecidos, sendo encontrado antÃgeno viral em 17 fetos positivos para PCV2 por PCR. As amostras de tecido reprodutivo das fÃmeas tambÃm foram testadas por PCR e o PCV2 foi identificado em 4 porcas (4,8%). Visando a detecÃÃo de TTV foram testadas por PCR amostras de ÃrgÃos reprodutivos de fÃmeas suÃnas, sendo diagnosticados os dois genogrupos de TTV, TTV1 e TTV2 em 25 (30,1%) e 41 (49,3%) fÃmeas, respectivamente. As amostras de fetos que resultaram positivas para PCV2 pela tÃcnica de PCR tambÃm foram testadas para TTV, observando-se a ocorrÃncia de coinfecÃÃo entre estes agentes. Os resultados obtidos evidenciam o provÃvel envolvimento do PCV2 em falhas reprodutivas em fÃmeas suÃnas, bem como mostram que o TTV està presente nas amostras analisadas, confirmando a associaÃÃo com o PCV2
Many infectious agents have been associated with reproductive failure in swine, representing significantly economic losses for production. Recently, Porcine Circovirus Type 2 (PCV2), etiologic agent of PCVAD or PCV2 associated diseases, was associated with reproductive failure in swine around the world. To confirm the pathogenic potential of PCV2 inducing reproductive failure in sows, itâs necessary the viral isolation and antigen and nucleic acid demonstration in fetuses. Other viral…
Advisors/Committee Members: Janice Reis Ciacci Zanella.
Subjects/Keywords: torque teno virus (TTV); MEDICINA VETERINARIA; circovÃrus suÃno tipo 2 (PCV2); fetos; falhas reprodutivas; PCR; torque teno vÃrus (TTV); porcine circovirus type 2; fetuses; PCR; reproductive failure
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❌
APA ·
Chicago ·
MLA ·
Vancouver ·
CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Ritterbusch, G. A. (2009). Pathogenicity study and co-infection investigation by Porcine Circovirus and Swine Torque Teno Virus in materials from sows with reproductive failure. (Thesis). Universidade do Estado de Santa Catarina. Retrieved from http://www.tede.udesc.br/tde_busca/arquivo.php?codArquivo=3363
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Ritterbusch, Giseli Aparecida. “Pathogenicity study and co-infection investigation by Porcine Circovirus and Swine Torque Teno Virus in materials from sows with reproductive failure.” 2009. Thesis, Universidade do Estado de Santa Catarina. Accessed January 16, 2021.
http://www.tede.udesc.br/tde_busca/arquivo.php?codArquivo=3363.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Ritterbusch, Giseli Aparecida. “Pathogenicity study and co-infection investigation by Porcine Circovirus and Swine Torque Teno Virus in materials from sows with reproductive failure.” 2009. Web. 16 Jan 2021.
Vancouver:
Ritterbusch GA. Pathogenicity study and co-infection investigation by Porcine Circovirus and Swine Torque Teno Virus in materials from sows with reproductive failure. [Internet] [Thesis]. Universidade do Estado de Santa Catarina; 2009. [cited 2021 Jan 16].
Available from: http://www.tede.udesc.br/tde_busca/arquivo.php?codArquivo=3363.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Ritterbusch GA. Pathogenicity study and co-infection investigation by Porcine Circovirus and Swine Torque Teno Virus in materials from sows with reproductive failure. [Thesis]. Universidade do Estado de Santa Catarina; 2009. Available from: http://www.tede.udesc.br/tde_busca/arquivo.php?codArquivo=3363
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Texas Medical Center
4.
Gabitzsch, Emily.
Prevalence of Premature Ovarian Failure in Women with Tuberous Sclerosis.
Degree: MS, 2011, Texas Medical Center
URL: https://digitalcommons.library.tmc.edu/utgsbs_dissertations/141
► Tuberous Sclerosis Complex (TSC) is an autosomal dominant tumor suppressor disorder characterized by hamartomas, or benign growths, in various organ systems. Inactivating mutations in…
(more)
▼ Tuberous Sclerosis Complex (TSC) is an autosomal dominant tumor suppressor disorder characterized by hamartomas, or benign growths, in various organ systems. Inactivating mutations in either the
TSC1 or the
TSC2 gene cause most cases of TSC. Recently, the use of ovarian specific conditional knock-out mouse models has demonstrated a crucial role of the TSC genes in ovarian function. Mice with complete deletion of
Tsc1 or
Tsc2 showed accelerated ovarian follicle activation and subsequent premature follicular depletion, consistent with the human condition premature ovarian
failure (POF). POF is defined in women as the cessation of menses before the age of 40 and elevated levels of follicle stimulating hormone (FSH). The prevalence of POF is estimated to be 1%, affecting a substantial number of women in the general population. Nonetheless, the etiology of most cases of POF remains unknown. Based on the mouse model results, we hypothesized that the human TSC1 and TSC2 genes are likely to be crucial for ovarian development and function. Moreover, since women with TSC already have one inactivated TSC gene, we further hypothesized that they may show a higher prevalence of POF. To test this hypothesis, we surveyed 1000 women with TSC belonging to the Tuberous Sclerosis Alliance, a national support organization. 182 questionnaires were analyzed for information on menstrual and
reproductive function, as well as TSC. This self-reported data revealed 8 women (4.4%) with possible POF, as determined by menstrual history report and additional supportive data. This prevalence is much higher than 1% in the general population. Data from all women suggested other
reproductive pathology associated with TSC such as a high rate of miscarriage (41.2%) and menstrual irregularity of any kind (31.2%). These results establish a previously unappreciated effect of TSC on women’s
reproductive health. Moreover, these data suggest that perturbations in the cellular pathways regulated by the TSC genes may play an important role in
reproductive function.
Advisors/Committee Members: Michael J. Gambello, MD, PhD, Hope Northrup, MD, Shala Nader-Eftekhari, MD.
Subjects/Keywords: Tuberous Sclerosis Complex; Premature Ovarian Failure; TSC; TSC1; TSC2; POF; Reproductiev; mTORC; Endocrinology, Diabetes, and Metabolism; Genetics; Medical Genetics; Molecular Genetics; Neurosciences; Other Medical Specialties; Reproductive and Urinary Physiology
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❌
APA ·
Chicago ·
MLA ·
Vancouver ·
CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Gabitzsch, E. (2011). Prevalence of Premature Ovarian Failure in Women with Tuberous Sclerosis. (Thesis). Texas Medical Center. Retrieved from https://digitalcommons.library.tmc.edu/utgsbs_dissertations/141
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Gabitzsch, Emily. “Prevalence of Premature Ovarian Failure in Women with Tuberous Sclerosis.” 2011. Thesis, Texas Medical Center. Accessed January 16, 2021.
https://digitalcommons.library.tmc.edu/utgsbs_dissertations/141.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Gabitzsch, Emily. “Prevalence of Premature Ovarian Failure in Women with Tuberous Sclerosis.” 2011. Web. 16 Jan 2021.
Vancouver:
Gabitzsch E. Prevalence of Premature Ovarian Failure in Women with Tuberous Sclerosis. [Internet] [Thesis]. Texas Medical Center; 2011. [cited 2021 Jan 16].
Available from: https://digitalcommons.library.tmc.edu/utgsbs_dissertations/141.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Gabitzsch E. Prevalence of Premature Ovarian Failure in Women with Tuberous Sclerosis. [Thesis]. Texas Medical Center; 2011. Available from: https://digitalcommons.library.tmc.edu/utgsbs_dissertations/141
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Canterbury
5.
Burrows, Ben Robert.
Do Severe Genetic Bottlenecks Lead to Greater Reproductive Failure?.
Degree: MS, Zoology, 2006, University of Canterbury
URL: http://dx.doi.org/10.26021/5619
► It is generally accepted that populations which experience severe bottlenecks have a reduction in fitness. One of the most frequently reported fitness costs is increased…
(more)
▼ It is generally accepted that populations which experience severe bottlenecks have a reduction in fitness. One of the most frequently reported fitness costs is increased hatching failure in bottlenecked populations of birds. The mechanism responsible for increased hatching failure is unknown. Research on other animals suggest that reduced population numbers cause unavoidable inbreeding that in turn leads to abnormalities in the gametes. In this thesis I examine some of the possible causes for increased hatching failure in severely bottlenecked populations of introduced birds in New Zealand. I look at three traits identified as a cause for infertility or hatching failure previously and determine whether there is a link with the size of a population s bottleneck. It is possible that reduced numbers of sperm reaching the site of fertilisation is a primary cause of hatching failure. I examined the perivitelline membrane of various species of introduced birds and counted the total number of sperm present to compare to how many would be expected in non-bottlenecked species. Although there was no relationship between the size of the bottleneck and the number of sperm present, all species had lower than expected sperm counts. In many species of mammals, a reduction in the quality of sperm is attributed to inbreeding depression bought about by genetic bottlenecks. I next compared the level of sperm abnormalities, variation in midpiece size sperm, and sperm motility with the size of the bottleneck each species passed through when introduced to New Zealand. There was no significant correlation between either the variation in midpiece size or sperm motility with bottleneck size. However, there was a trend for species that passed through more severe bottlenecks to have a slightly higher level of midpiece size and lower motility. Finally, I examined whether there was a link between abnormalities in the eggshell and the size of the respective bottleneck. There was no significant change in eggshell thickness or any change in the number of pores associated bottleneck size. However, there was a decreased number of round pores in severely bottlenecked species, although the consequences of this are unknown. My findings do not directly link a single cause for increased hatching failure in bottlenecked species of birds, but they do highlight the need for monitoring of reproductive traits in endangered species that have experienced a population bottleneck.
Subjects/Keywords: Genetic Bottleneck; reproductive failure; conservation; sperm; eggshell; New Zealand conservation; inbreeding depression
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❌
APA ·
Chicago ·
MLA ·
Vancouver ·
CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Burrows, B. R. (2006). Do Severe Genetic Bottlenecks Lead to Greater Reproductive Failure?. (Masters Thesis). University of Canterbury. Retrieved from http://dx.doi.org/10.26021/5619
Chicago Manual of Style (16th Edition):
Burrows, Ben Robert. “Do Severe Genetic Bottlenecks Lead to Greater Reproductive Failure?.” 2006. Masters Thesis, University of Canterbury. Accessed January 16, 2021.
http://dx.doi.org/10.26021/5619.
MLA Handbook (7th Edition):
Burrows, Ben Robert. “Do Severe Genetic Bottlenecks Lead to Greater Reproductive Failure?.” 2006. Web. 16 Jan 2021.
Vancouver:
Burrows BR. Do Severe Genetic Bottlenecks Lead to Greater Reproductive Failure?. [Internet] [Masters thesis]. University of Canterbury; 2006. [cited 2021 Jan 16].
Available from: http://dx.doi.org/10.26021/5619.
Council of Science Editors:
Burrows BR. Do Severe Genetic Bottlenecks Lead to Greater Reproductive Failure?. [Masters Thesis]. University of Canterbury; 2006. Available from: http://dx.doi.org/10.26021/5619
6.
Camila AndrÃa Salvitti de SÃ Rocha.
DetecÃÃo de parvovÃrus suÃno em material proveniente de porcas com patologias reprodutivas.
Degree: 2010, Universidade do Estado de Santa Catarina
URL: http://www.tede.udesc.br/tde_busca/arquivo.php?codArquivo=3353
► A parvovirose suÃna à uma doenÃa causadora de falhas reprodutivas e seu agente etiolÃgico à o ParvovÃrus SuÃno Tipo 1 (PVS1). Essa enfermidade, de alta…
(more)
▼ A parvovirose suÃna à uma doenÃa causadora de falhas reprodutivas e seu agente etiolÃgico à o ParvovÃrus SuÃno Tipo 1 (PVS1). Essa enfermidade, de alta prevalÃncia e distribuiÃÃo mundial, afeta principalmente porcas nulÃparas nÃo imunes. Clinicamente observa-se retorno ao cio, atraso na data de pariÃÃo, nascimento de leitÃes inviÃveis, fracos, natimortos e, principalmente, mumificados em diferentes tamanhos. Estes problemas reprodutivos acarretam prejuÃzos econÃmicos demonstrados pelos baixos Ãndices produtivos. O presente trabalho objetivou implantar e validar tÃcnicas de diagnÃstico laboratorial para detecÃÃo de PVS1 em infecÃÃes naturais em rebanhos suÃnos que apresentam problemas reprodutivos e realizar caracterizaÃÃo desses isolados. Mais especificamente, objetivou-se diagnosticar, atravÃs de nested-PCR, e caracterizar, atravÃs de anÃlise filogenÃtica, isolados de PVS1 em amostras de tecidos e lÃquido estomacal de fetos e em aparelho reprodutivo de porcas descartadas. Objetivou-se ainda avaliar histologicamente os tecidos que resultaram positivos na nested-PCR; padronizar um controle interno de amplificaÃÃo (CIA) para a nested-PCR de PVS1; padronizar tÃcnica de imunohistoquÃmica (IHQ) em tecidos fetais e padronizar PCR para ParvovÃrus SuÃno Tipo 4 (PVS4). De 27 rebanhos de suÃnos, foram colhidos 230 fetos entre natimortos, mumificados e abortados, os quais foram necropsiados para colheita de ÃrgÃos e lÃquido estomacal. O DNA viral foi detectado por nested-PCR em ÃrgÃos de seis (2,61%) fetos dos 230 analisados, sendo o cerebelo e a medula os ÃrgÃos onde o vÃrus foi detectado com maior frequÃncia (50%). LÃquido estomacal de trÃs (2,75%) fetos foram positivos por nested-PCR, de 109 analisados. AlÃm disso, amostras de ÃrgÃos reprodutivos de 83 porcas descartadas foram colhidas e tambÃm fluido folicular ovariano de 71 delas. O material genÃtico do PVS1 foi diagnosticado em ÃrgÃos de seis porcas (7,23%) e em fluido folicular de trÃs (4,22%) delas. De 19 ÃrgÃos positivos de fetos na nested-PCR, quatro tiveram lesÃo histolÃgica. De seis ovÃrios e seis Ãteros das seis fÃmeas suÃnas descartadas positivas na nested-PCR, cinco ovÃrios estavam ciclando, um estava em anestro e quatro Ãteros tinham algum grau de endometrite. O CIA desenvolvido para a nested-PCR foi amplificado em todas as reaÃÃes, conforme o esperado. Nos testes de IHQ a presenÃa do vÃrus foi confirmada em tecido fetal. A PCR para o PVS4 foi padronizada e testada em ÃrgÃos fetais e de porcas descartadas. Algumas amostras de porcas foram positivas para este novo parvovÃrus suÃno. Os resultados obtidos no estudo evidenciam baixa freqÃÃncia do PVS1 nos rebanhos analisados, bem como nos ÃrgÃos reprodutivos de porcas descartadas, considerando baixo o envolvimento do PVS1 nas falhas reprodutivas. AlÃm disso, as tÃcnicas padronizadas podem ser incorporadas na rotina de diagnÃstico de PVS1 e PVS4
Porcine parvovirosis is a disease that causes reproductive failure and its causative agent is the Porcine Parvovirus Type 1 (PPV1). This disease, with high prevalence and…
Advisors/Committee Members: Janice Reis Ciacci Zanella.
Subjects/Keywords: parvovÃrus suÃno tipo 1 (PVS1); falhas reprodutivas; fetos; porcas descartadas; nested-PCR; parvovÃrus suÃno tipo 4 (PVS4); imunohistoquÃmica; porcine parvovirus type 1 (PPV1); reproductive failure; fetuses; culled sow; nested-PCR; porcinepParvovirus type 4 (PPV4); immunohistochemistry; MEDICINA VETERINARIA
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to Zotero / EndNote / Reference
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APA (6th Edition):
Rocha, C. A. S. d. S. (2010). DetecÃÃo de parvovÃrus suÃno em material proveniente de porcas com patologias reprodutivas. (Thesis). Universidade do Estado de Santa Catarina. Retrieved from http://www.tede.udesc.br/tde_busca/arquivo.php?codArquivo=3353
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Rocha, Camila AndrÃa Salvitti de SÃ. “DetecÃÃo de parvovÃrus suÃno em material proveniente de porcas com patologias reprodutivas.” 2010. Thesis, Universidade do Estado de Santa Catarina. Accessed January 16, 2021.
http://www.tede.udesc.br/tde_busca/arquivo.php?codArquivo=3353.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Rocha, Camila AndrÃa Salvitti de SÃ. “DetecÃÃo de parvovÃrus suÃno em material proveniente de porcas com patologias reprodutivas.” 2010. Web. 16 Jan 2021.
Vancouver:
Rocha CASdS. DetecÃÃo de parvovÃrus suÃno em material proveniente de porcas com patologias reprodutivas. [Internet] [Thesis]. Universidade do Estado de Santa Catarina; 2010. [cited 2021 Jan 16].
Available from: http://www.tede.udesc.br/tde_busca/arquivo.php?codArquivo=3353.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Rocha CASdS. DetecÃÃo de parvovÃrus suÃno em material proveniente de porcas com patologias reprodutivas. [Thesis]. Universidade do Estado de Santa Catarina; 2010. Available from: http://www.tede.udesc.br/tde_busca/arquivo.php?codArquivo=3353
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Marshall University
7.
Ward, Dakota Blake.
Mechanistic Study of Diatrizoic Acid Induced Proximal Tubule Cytotoxicity.
Degree: 2019, Marshall University
URL: https://mds.marshall.edu/etd/1208
► Radiocontrast media (RCM) are necessary for many diagnostic procedures such as arteriography, percutaneous coronary intervention (PCI), and computed tomography. Contrast-induced acute kidney injury (CI-AKI) is…
(more)
▼ Radiocontrast media (RCM) are necessary for many diagnostic procedures such as arteriography, percutaneous coronary intervention (PCI), and computed tomography. Contrast-induced acute kidney injury (CI-AKI) is the third most common cause of hospital-associated kidney damage accounting for 10-25% of cases worldwide. The mechanisms of contrast-induced renal impairment are not entirely known, but diminished renal hemodynamics, inflammatory responses, and direct cytotoxicity have been hypothesized. The purpose of this study was to investigate the mechanisms of direct cytotoxicity observed in HK-2 cells following treatment with diatrizoic acid (DA) and to determine the source of this damage. Mitochondrial function, endoplasmic reticulum (ER) stress, oxidative stress, and the role of calcium were examined in response to exposure to DA. HK-2 cells were grown to confluency for 48 hr and exposed to 0-30 mg I/mL of DA for 2, 8, or 24 hr. The vehicle used for all studies was phosphate buffered saline (PBS). Mitochondrial and cell viability were decreased within 2 and 24 hr, respectively, as shown by MTT assays and trypan blue exclusion cell counts. Mitochondrial function was monitored using an Agilent Seahorse analyzer and cell mito stress tests, cell glycolysis stress tests, mito fuel flex tests, and real-time ATP rate assays. Oxidative stress, ER stress, and mitophagy were assessed in whole cell lysate and cell fractions using OxyBlot and Western blot analysis for 4-hydroxynonenol (4-HNE), tumor necrosis factor alpha (TNFα), NADPH oxidase 4 (NOX4), manganese superoxide dismutase (MnSOD), glucose-regulated protein 78 (GRP78), C/EBP homologous protein (CHOP), microtubule-associated proteins 1A/1B light chain 3B (LC3B), cytochrome c, and caspases 3, 4, and 12. The role of calcium in mitophagy and apoptosis was determined by pretreating HK-2 cells with various calcium modulators such as BAPTA-AM, EGTA, 2-APB, or calpeptin prior to the addition of DA. Studies conducted using Seahorse XF technology and analysis of LC3BI and II expression determined that DA alters mitochondrial function within 8 hr. MTT and calpain activity assays indicated that disruption of calcium homeostasis plays a role in DA induced cytotoxicity within 8 hr. An increase in oxidative stress and loss of mitochondrial membrane integrity was evident within 24 hr exposure to 18 mg I/mL DA. DA induces apoptosis at 24 hr exposure as shown by Western blot analysis of cytochrome c leakage and activation of caspase 3 and 12. These studies indicate that mitochondrial damage and oxidative stress occur in HK-2 cells treated with DA, and maintaining calcium homeostasis may help prevent DA-induced cytotoxicity.
Subjects/Keywords: contrast-induced acute kidney injury; diatrizoic acid; mitophagy; oxidative stress; proximal tubule cytotoxicity; Seahorse XFe; <; p>; Acute renal failure.<; /p>;
<; p>; Cytokines – Research.<; /p>;
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❌
APA ·
Chicago ·
MLA ·
Vancouver ·
CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Ward, D. B. (2019). Mechanistic Study of Diatrizoic Acid Induced Proximal Tubule Cytotoxicity. (Thesis). Marshall University. Retrieved from https://mds.marshall.edu/etd/1208
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Ward, Dakota Blake. “Mechanistic Study of Diatrizoic Acid Induced Proximal Tubule Cytotoxicity.” 2019. Thesis, Marshall University. Accessed January 16, 2021.
https://mds.marshall.edu/etd/1208.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Ward, Dakota Blake. “Mechanistic Study of Diatrizoic Acid Induced Proximal Tubule Cytotoxicity.” 2019. Web. 16 Jan 2021.
Vancouver:
Ward DB. Mechanistic Study of Diatrizoic Acid Induced Proximal Tubule Cytotoxicity. [Internet] [Thesis]. Marshall University; 2019. [cited 2021 Jan 16].
Available from: https://mds.marshall.edu/etd/1208.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Ward DB. Mechanistic Study of Diatrizoic Acid Induced Proximal Tubule Cytotoxicity. [Thesis]. Marshall University; 2019. Available from: https://mds.marshall.edu/etd/1208
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Lund
8.
Bungum, Mona.
Studies on genetic aberrations as possible predictors of
the outcome of assisted reproduction.
Degree: 2008, University of Lund
URL: https://lup.lub.lu.se/record/1213344
;
https://portal.research.lu.se/ws/files/3599758/1213359.pdf
► Traditionally, diagnosis of male infertility has relied upon microscopic assessment of semen. The normality criteria set by the World Health Organization (WHO) in regard to…
(more)
▼ Traditionally, diagnosis of male infertility has
relied upon microscopic assessment of semen. The normality criteria
set by the World Health Organization (WHO) in regard to sperm
concentration, motility and morphology are, however, poor
predictors of fertility. In about half of the involuntarily
childless couples the causes are solely or partly male related. In
60-75% of cases the aetiology of reduced semen quality remains
unexplained and is referred to as being idiopathic. In recent years
fragmentation of sperm DNA and/or genetic polymorphisms have
attracted an increased interest in seeking for causes of male
mediated infertility. However, when the work behind this thesis was
initiated, the knowledge regarding the impact of somatic and germ
cells genetic aberrations, in relation to in vivo and in vitro
fertility, was rather limited. Many of the involuntarily childless
couples seek for assisted reproduction (ART), however, the
“baby-take-home rates” in ART are still relatively low. One of the
reasons for this is a lack of adequate methods to evaluate the
fertility potential of a couple and also a lack of methods to
identify the most effective type of ART treatment for a given
couple. Thus, the overall aim of these studies was to identify
genetic markers with a potential to predict the chance of success
or failure of ART. The present studies demonstrated that DNA
fragmentation index (DFI), as measured by the sperm chromatin
structure assay (SCSA), can be used as an independent predictor of
fertility in couples undergoing intrauterine insemination (IUI). If
DFI is above 30% the chance of pregnancy by IUI is close to zero
and intracytoplasmic sperm injection (ICSI) is more effective than
classical in vitro fertilisation (IVF).DFI was predictive of the
outcome of ART only when measured in neat semen, not in density
gradient centrifuged. Moreover, the present studies have shown
that, like the other sperm parameters, DFI is subject to a
significant intra-individual variation, with a coefficient of
variation of 29%. Finally, it was found that in men from couples
experiencing IVF fertilisation failure a specific polymorphism in
the Protein C Inhibitor (PCI) gene is significantly more common
compared to cases with normal fertilisation rates after IVF. The
results indicate that in a subgroup of cases total fertilisation
failure may be caused by polymorphisms in the PCI gene. In
conclusion the present studies have demonstrated that both somatic
and sperm genetic aberrations can be used as markers of the outcome
of ART.
Subjects/Keywords: Obstetrics, Gynecology and Reproductive
Medicine; assisted reproduction; IUI; Sperm chromatin; ICSI; intra-individual variation; fertilisation failure genetic aberration; Protein C inhibitor gene; sperm DNA damage SCSA; IVF
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❌
APA ·
Chicago ·
MLA ·
Vancouver ·
CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Bungum, M. (2008). Studies on genetic aberrations as possible predictors of
the outcome of assisted reproduction. (Doctoral Dissertation). University of Lund. Retrieved from https://lup.lub.lu.se/record/1213344 ; https://portal.research.lu.se/ws/files/3599758/1213359.pdf
Chicago Manual of Style (16th Edition):
Bungum, Mona. “Studies on genetic aberrations as possible predictors of
the outcome of assisted reproduction.” 2008. Doctoral Dissertation, University of Lund. Accessed January 16, 2021.
https://lup.lub.lu.se/record/1213344 ; https://portal.research.lu.se/ws/files/3599758/1213359.pdf.
MLA Handbook (7th Edition):
Bungum, Mona. “Studies on genetic aberrations as possible predictors of
the outcome of assisted reproduction.” 2008. Web. 16 Jan 2021.
Vancouver:
Bungum M. Studies on genetic aberrations as possible predictors of
the outcome of assisted reproduction. [Internet] [Doctoral dissertation]. University of Lund; 2008. [cited 2021 Jan 16].
Available from: https://lup.lub.lu.se/record/1213344 ; https://portal.research.lu.se/ws/files/3599758/1213359.pdf.
Council of Science Editors:
Bungum M. Studies on genetic aberrations as possible predictors of
the outcome of assisted reproduction. [Doctoral Dissertation]. University of Lund; 2008. Available from: https://lup.lub.lu.se/record/1213344 ; https://portal.research.lu.se/ws/files/3599758/1213359.pdf
9.
McKenzie, Jane.
Population demographics of New Zealand fur seals (Arctocephalus forsteri).
Degree: 2006, La Trobe University
URL: http://www.lib.latrobe.edu.au./thesis/public/adt-LTU20080509.121141
► Assessment of trophic interactions between increasing populations of New Zealand fur seals (Arctocephalus forsteri) and fisheries in southern Australia is limited due to a lack…
(more)
▼ Assessment of trophic interactions between increasing populations of New Zealand fur seals (Arctocephalus forsteri) and fisheries in southern Australia is limited due to a lack of species specific demographic data and an understanding of the factors influencing population growth. To establish species specific demographic parameters a cross-sectional sample of New Zealand fur seal females (330) and males (100) were caught and individually-marked on Kangaroo Island, South Australia between 2000 and 2003. The seals were aged through examination of a postcanine tooth, which was removed from each animal to investigate age-specific life-history parameters. Annual formation of cementum layers was confirmed and accuracy in age estimation was determined by examination of teeth removed from individuals of known-age. Indirect methods of assessing reproductive maturity based on mammary teat characteristics indicated that females first gave birth between 4-8 years of age, with an average age at reproductive maturity of 5 years. Among reproductively mature females, age-specific reproductive rates increased rapidly between 4-7 years of age, reaching maximum rates of 70-81% between 8-13 years, and gradually decreased in older females. No females older than 22 years were recorded to pup. Age of first territory tenure in males ranged from 8-10 years. The oldest female and male were 25 and 19 years old, respectively. Post-weaning growth in females was monophasic, characterised by high growth rates in length and mass during the juvenile growth stage, followed by a gradual decline in growth rates after reproductive maturity. In contrast, growth in males was biphasic and displayed a secondary growth spurt in both length and mass, which coincided with sexual and social maturation, followed by a rapid decline in growth rates. Age-specific survival rates were high (0.823-0.953) among prime-age females (8-13 yrs of age) and declined in older females. Relative change in annual pup production was strongly correlated with reproductive rates of prime-age females and adult female survival between breeding seasons.
Subjects/Keywords: New Zealand fur seal; Kangaroo Island - South Australia; New Zealand fur seal - Population viability analysis; New Zealand fur seal - Breeding; Fisheries - South Australia - Environmental variability; Marine mammal populations; Pinniped; otarid; remote chemical immobilization; darting; anaesthesia; isoflurane; midazolam; zoletil; tiletamine-zolazepam; restraint; behavioral response; fecundity; pregnancy rates; progesterone; reproduction; reproductive failure; site fidelity; mortality; territorial; size dimorphism; life table; ageing; growth layer groups; re-colonization
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❌
APA ·
Chicago ·
MLA ·
Vancouver ·
CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
McKenzie, J. (2006). Population demographics of New Zealand fur seals (Arctocephalus forsteri). (Thesis). La Trobe University. Retrieved from http://www.lib.latrobe.edu.au./thesis/public/adt-LTU20080509.121141
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
McKenzie, Jane. “Population demographics of New Zealand fur seals (Arctocephalus forsteri).” 2006. Thesis, La Trobe University. Accessed January 16, 2021.
http://www.lib.latrobe.edu.au./thesis/public/adt-LTU20080509.121141.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
McKenzie, Jane. “Population demographics of New Zealand fur seals (Arctocephalus forsteri).” 2006. Web. 16 Jan 2021.
Vancouver:
McKenzie J. Population demographics of New Zealand fur seals (Arctocephalus forsteri). [Internet] [Thesis]. La Trobe University; 2006. [cited 2021 Jan 16].
Available from: http://www.lib.latrobe.edu.au./thesis/public/adt-LTU20080509.121141.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
McKenzie J. Population demographics of New Zealand fur seals (Arctocephalus forsteri). [Thesis]. La Trobe University; 2006. Available from: http://www.lib.latrobe.edu.au./thesis/public/adt-LTU20080509.121141
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Latrobe University
10.
McKenzie, Jane.
Population demographics of New Zealand fur seals (Arctocephalus forsteri).
Degree: 2006, Latrobe University
URL: http://hdl.handle.net/1959.9/468696
► Assessment of trophic interactions between increasing populations of New Zealand fur seals (Arctocephalus forsteri) and fisheries in southern Australia is limited due to a lack…
(more)
▼ Assessment of trophic interactions between increasing populations of New Zealand fur seals (Arctocephalus forsteri) and fisheries in southern Australia is limited due to a lack of species specific demographic data and an understanding of the factors influencing population growth. To establish species specific demographic parameters a cross-sectional sample of New Zealand fur seal females (330) and males (100) were caught and individually-marked on Kangaroo Island, South Australia between 2000 and 2003. The seals were aged through examination of a postcanine tooth, which was removed from each animal to investigate age-specific life-history parameters. Annual formation of cementum layers was confirmed and accuracy in age estimation was determined by examination of teeth removed from individuals of known-age.Indirect methods of assessing reproductive maturity based on mammary teat characteristics indicated that females first gave birth between 4-8 years of age, with an average age at reproductive maturity of 5 years. Among reproductively mature females, age-specific reproductive rates increased rapidly between 4-7 years of age, reaching maximum rates of 70-81% between 8-13 years, and gradually decreased in older females. No females older than 22 years were recorded to pup. Age of first territory tenure in males ranged from 8-10 years. The oldest female and male were 25 and 19 years old, respectively. Post-weaning growth in females was monophasic, characterised by high growth rates in length and mass during the juvenile growth stage, followed by a gradual decline in growth rates after reproductive maturity.In contrast, growth in males was biphasic and displayed a secondary growth spurt in both length and mass, which coincided with sexual and social maturation, followed by a rapid decline in growth rates. Age-specific survival rates were high (0.823-0.953) among prime-age females (8-13yrs of age) and declined in older females. Relative change in annual pup production was strongly correlated with reproductive rates of prime-age females and adult female survival between breeding seasons.
Subjects/Keywords: Pinniped; otarid; remote chemical immobilization; darting; anaesthesia; isoflurane; midazolam; zoletil; tiletamine-zolazepam; restraint; behavioral response; fecundity; pregnancy rates; progesterone; reproduction; reproductive failure; site fidelity; mortality; territorial; size dimorphism; life table; ageing; growth layer groups; re-colonization; New Zealand fur seal – South Australia – Kangaroo Island; New Zealand fur seal – Population viability analysis – South Australia – Kangaroo Island; New Zealand fur seal – Breeding – South Australia – Kangaroo Island; Fisheries – South Australia – Environmental aspects; Marine mammal populations – South Australia – Kangaroo Island
Record Details
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Record Details
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❌
APA ·
Chicago ·
MLA ·
Vancouver ·
CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
McKenzie, J. (2006). Population demographics of New Zealand fur seals (Arctocephalus forsteri). (Thesis). Latrobe University. Retrieved from http://hdl.handle.net/1959.9/468696
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
McKenzie, Jane. “Population demographics of New Zealand fur seals (Arctocephalus forsteri).” 2006. Thesis, Latrobe University. Accessed January 16, 2021.
http://hdl.handle.net/1959.9/468696.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
McKenzie, Jane. “Population demographics of New Zealand fur seals (Arctocephalus forsteri).” 2006. Web. 16 Jan 2021.
Vancouver:
McKenzie J. Population demographics of New Zealand fur seals (Arctocephalus forsteri). [Internet] [Thesis]. Latrobe University; 2006. [cited 2021 Jan 16].
Available from: http://hdl.handle.net/1959.9/468696.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
McKenzie J. Population demographics of New Zealand fur seals (Arctocephalus forsteri). [Thesis]. Latrobe University; 2006. Available from: http://hdl.handle.net/1959.9/468696
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Otago
11.
Wylie, Matthew John.
Reproductive physiology of wreckfish (hāpuku) Polyprion oxygeneios in captivity
.
Degree: University of Otago
URL: http://hdl.handle.net/10523/7414
► Summary New Zealand’s flagship aquaculture species are currently the green-lipped mussel (Perna canaliculus), the Pacific oyster (Crassostrea gigas) and the chinook salmon (Oncorhynchus tshawytscha). However,…
(more)
▼ Summary
New Zealand’s flagship aquaculture species are currently the green-lipped mussel (Perna canaliculus), the Pacific oyster (Crassostrea gigas) and the chinook salmon (Oncorhynchus tshawytscha). However, important drivers to achieve growth of the aquaculture sector have been identified through the diversification of species being cultured and the development of innovative products. In response to this, the New Zealand National Institute of Water and Atmospheric Research (NIWA) has responded by conducting extensive research into the culture of the wreckfish species Polyprion oxygeneios – locally known as hāpuku or groper.
Information is limited the
reproductive biology, behaviour and life histories of both wild hāpuku and those maintained in captivity. In the wild, it is believed that breeding individuals migrate to deep and in many cases unfishable areas to spawn while wild-caught captivity-acclimated broodstock maintained under photo-thermal control communally spawn without hormonal intervention. However, despite the development of hatchery technologies to raise hāpuku larvae by NIWA, seed supply is still reliant on these wild-caught captivity-acclimated broodstock and significant issues bottlenecking production include variable egg quality and poor larval survival.
It is now evident that the development of appropriate broodstock management strategies for an emerging aquaculture species like hāpuku requires the ability to identify, monitor and control ovarian growth, oocyte maturation and ovulation in stock. Research within this thesis makes a significant contribution to such requirements by studying first filial (F1) fish in captivity at various stages of the
reproductive cycle.
The first study has a basic focus examining ovarian development by RNA-seq on tissues at different stages oogenesis (see Chapter 2 for details). The aim of this study was provide a snapshot of the composite changes in gene expression associated with oocyte growth in hāpuku – particularly during the transition from previtellogenic to early vitellogenic stages of ovarian growth. Thirty-five differentially expressed genes were identified and upregulated between previtellogenic and early vitellogenic stages of development. Once these genes were grouped according to tentative functions, those associated with the respiratory electron transport chain, lipid metabolism, steroidogenesis and mineral/ solute transport were apparent.
The second study centred around advancing puberty by examining the effects of several sex steroid and neuropeptide treatments on the pituitary-gonadal axis of pre-pubertal hāpuku in vivo (see Chapter 3 for details). While puberty could not be advanced, outcomes from this study included insights into the negative feedback effects of androgens on the pituitary-gonadal axis of pre-pubertal hāpuku and directions for future research towards the exploration of alternative treatments to advance puberty.
In order to develop more appropriate broodstock management strategies, Chapter 4 aimed to describe ovarian…
Advisors/Committee Members: Lokman, Pieter Mark (advisor).
Subjects/Keywords: BPG axis;
wreckfish;
groper;
hapuku;
hapuka;
aquaculture;
GnRHa;
oocyte;
Polyprion;
vitellogenesis;
kisspeptin;
spawning;
androgen;
estrogen;
oestrogen;
11KT;
E2;
oogenesis;
F1;
gonadotropin;
Lh;
Fsh;
Transcriptome;
RNA-seq;
follicle-stimulating hormone;
ovary;
pituitary;
kiss;
luteinizing hormone;
brain;
gametogenesis;
EVac;
steroidogenesis;
puberty;
finfish;
broodstock;
temperature;
photoperiod;
plasma;
RT-PCR;
mRNA;
cycle;
reproductive;
oxygeneios;
NIWA;
egg;
egg quality;
induction;
gnrhr;
lhr;
fshr;
aromatase;
cyp19a;
receptor;
beta;
glycoprotein;
feedback;
physiology;
cativity;
maturity;
maturation;
yolk;
lipid;
gamete;
life-cycle closure;
F2;
HiSeq;
previtellogenic;
final oocyte maturation;
fish;
teleost;
perciform;
ovaries;
ovulation;
pre-pubertal;
kiss2;
dummy run;
maiden;
biopsy;
cannulation;
failure;
seed;
dysfunction
Record Details
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Record Details
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❌
APA ·
Chicago ·
MLA ·
Vancouver ·
CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Wylie, M. J. (n.d.). Reproductive physiology of wreckfish (hāpuku) Polyprion oxygeneios in captivity
. (Doctoral Dissertation). University of Otago. Retrieved from http://hdl.handle.net/10523/7414
Note: this citation may be lacking information needed for this citation format:
No year of publication.
Chicago Manual of Style (16th Edition):
Wylie, Matthew John. “Reproductive physiology of wreckfish (hāpuku) Polyprion oxygeneios in captivity
.” Doctoral Dissertation, University of Otago. Accessed January 16, 2021.
http://hdl.handle.net/10523/7414.
Note: this citation may be lacking information needed for this citation format:
No year of publication.
MLA Handbook (7th Edition):
Wylie, Matthew John. “Reproductive physiology of wreckfish (hāpuku) Polyprion oxygeneios in captivity
.” Web. 16 Jan 2021.
Note: this citation may be lacking information needed for this citation format:
No year of publication.
Vancouver:
Wylie MJ. Reproductive physiology of wreckfish (hāpuku) Polyprion oxygeneios in captivity
. [Internet] [Doctoral dissertation]. University of Otago; [cited 2021 Jan 16].
Available from: http://hdl.handle.net/10523/7414.
Note: this citation may be lacking information needed for this citation format:
No year of publication.
Council of Science Editors:
Wylie MJ. Reproductive physiology of wreckfish (hāpuku) Polyprion oxygeneios in captivity
. [Doctoral Dissertation]. University of Otago; Available from: http://hdl.handle.net/10523/7414
Note: this citation may be lacking information needed for this citation format:
No year of publication.
.