subject:(replication initiation protein Rep )

Queensland University of Technology

1. Tsao, Theresa Tsun-Hui. Towards the development of transgenic banana bunchy top virus (BBTV)-resistant banana plants : interference with replication.

Degree: 2008, Queensland University of Technology

URL: https://eprints.qut.edu.au/17031/

► Banana bunchy top virus (BBTV) causes one of the most devastating diseases of banana. Transgenic virus resistance is now considered one of the most promising… (more)

Subjects/Keywords: banana bunchy top virus; nanoviruses; replication initiation protein (Rep); pathogen-derived resistance; BBTV DNA-R; BBTV DNA-S1; BBTV DNA-S3; BBTV DNA-S4; satellite DNA; ATPase; post-transcriptional gene silencing; RNA silencing suppressor

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APA (6^th Edition):

Tsao, T. T. (2008). Towards the development of transgenic banana bunchy top virus (BBTV)-resistant banana plants : interference with replication. (Thesis). Queensland University of Technology. Retrieved from https://eprints.qut.edu.au/17031/

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Tsao, Theresa Tsun-Hui. “Towards the development of transgenic banana bunchy top virus (BBTV)-resistant banana plants : interference with replication.” 2008. Thesis, Queensland University of Technology. Accessed March 01, 2021. https://eprints.qut.edu.au/17031/.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Tsao, Theresa Tsun-Hui. “Towards the development of transgenic banana bunchy top virus (BBTV)-resistant banana plants : interference with replication.” 2008. Web. 01 Mar 2021.

Vancouver:

Tsao TT. Towards the development of transgenic banana bunchy top virus (BBTV)-resistant banana plants : interference with replication. [Internet] [Thesis]. Queensland University of Technology; 2008. [cited 2021 Mar 01]. Available from: https://eprints.qut.edu.au/17031/.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Tsao TT. Towards the development of transgenic banana bunchy top virus (BBTV)-resistant banana plants : interference with replication. [Thesis]. Queensland University of Technology; 2008. Available from: https://eprints.qut.edu.au/17031/

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Queensland University of Technology

2. Hafner, Gregory. Replication of banana bunchy top virus : mechanisms and interference.

Degree: 1998, Queensland University of Technology

URL: http://eprints.qut.edu.au/36970/

Subjects/Keywords: Banana bunchy top disease; Bananas Diseases and pests; banana; bunchy top; virus; disease; transmission; movement; systemic spread; Pentalonia nigronervosa; genome; strand-initiation; self-priming; primer; Rep; replication; initiator; protein; nicking; joining; ribozyme; antisense; resistance; Musa; thesis; doctoral

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APA (6^th Edition):

Hafner, G. (1998). Replication of banana bunchy top virus : mechanisms and interference. (Thesis). Queensland University of Technology. Retrieved from http://eprints.qut.edu.au/36970/

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Hafner, Gregory. “Replication of banana bunchy top virus : mechanisms and interference.” 1998. Thesis, Queensland University of Technology. Accessed March 01, 2021. http://eprints.qut.edu.au/36970/.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Hafner, Gregory. “Replication of banana bunchy top virus : mechanisms and interference.” 1998. Web. 01 Mar 2021.

Vancouver:

Hafner G. Replication of banana bunchy top virus : mechanisms and interference. [Internet] [Thesis]. Queensland University of Technology; 1998. [cited 2021 Mar 01]. Available from: http://eprints.qut.edu.au/36970/.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Hafner G. Replication of banana bunchy top virus : mechanisms and interference. [Thesis]. Queensland University of Technology; 1998. Available from: http://eprints.qut.edu.au/36970/

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Queensland University of Technology

3. Horser, Cathryn Louise. Characterisation of the putative satellite DNAs associated with banana bunchy top virus.

Degree: 2000, Queensland University of Technology

URL: http://eprints.qut.edu.au/37078/

Subjects/Keywords: Banana bunchy top disease; Bananas Diseases and pests; banana bunchy top virus (BBTV); circular single-stranded DNA(cssDNA); nanovirus; geminivirus; replication initiation protein (Rep); BBTV-S1; BBTV-S2; BBTV-Y1; FBNYV; MDV; SCSV; CFDV; thesis; doctoral

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APA (6^th Edition):

Horser, C. L. (2000). Characterisation of the putative satellite DNAs associated with banana bunchy top virus. (Thesis). Queensland University of Technology. Retrieved from http://eprints.qut.edu.au/37078/

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Horser, Cathryn Louise. “Characterisation of the putative satellite DNAs associated with banana bunchy top virus.” 2000. Thesis, Queensland University of Technology. Accessed March 01, 2021. http://eprints.qut.edu.au/37078/.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Horser, Cathryn Louise. “Characterisation of the putative satellite DNAs associated with banana bunchy top virus.” 2000. Web. 01 Mar 2021.

Vancouver:

Horser CL. Characterisation of the putative satellite DNAs associated with banana bunchy top virus. [Internet] [Thesis]. Queensland University of Technology; 2000. [cited 2021 Mar 01]. Available from: http://eprints.qut.edu.au/37078/.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Horser CL. Characterisation of the putative satellite DNAs associated with banana bunchy top virus. [Thesis]. Queensland University of Technology; 2000. Available from: http://eprints.qut.edu.au/37078/

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Vanderbilt University

4. Dhingra, Nalini. Elucidating the role of Dpb11 in replication initiation.

Degree: PhD, Biological Sciences, 2015, Vanderbilt University

URL: http://hdl.handle.net/1803/12863

► Initiation of DNA Replication is a highly regulated process and is characterized by the conversion of inactive Mcm2-7 double hexamer around dsDNA to an active… (more)

▼ Initiation of DNA Replication is a highly regulated process and is characterized by the conversion of inactive Mcm2-7 double hexamer around dsDNA to an active helicase consisting of Mcm2-7 single hexamer which encircles ssDNA along with Cdc45 and GINS. This transition of inactive Mcm2-7 around dsDNA to the active helicase around ssDNA involves several unknown mechanisms. Many proteins like, Sld2, Sld3, Dpb11, Mcm10, have been reported to be essential for replication initiation. Dpb11 is one of these essential proteins that functions in the initiation of DNA replication. Dpb11 binds to S-CDK phosphorylated Sld2 and Sld3 to form a ternary complex during S phase. These interactions are essential for the CDK-dependent activation of DNA replication in budding yeast. Studies have also indicated that chromosomal replication initiates by a fundamentally similar process in all eukaryotes, with Dpb11 being an evolutionary conserved protein. Our studies using purified proteins from budding yeast show that Dpb11 alone binds to Mcm2-7, and Dpb11 also competes with GINS for binding to Mcm2-7. Furthermore, Dpb11 binds directly to single-stranded DNA (ssDNA), and ssDNA inhibits Dpb11 interaction with Mcm2-7. We also found that Dpb11 can recruit Cdc45 to Mcm2-7. We identified a mutant of the BRCT4 motif of Dpb11 that remains bound to Mcm2-7 in the presence of ssDNA (dpb11-m1,m2,m3,m5), and this mutant exhibits a DNA replication defect when expressed in budding yeast cells. Expression of this mutant results in increased interaction between Dpb11 and Mcm2-7 during S phase, impaired GINS interaction with Mcm2-7 during S phase, and decreased RPA interaction with origin DNA during S phase. We propose a model wherein Dpb11 first recruits Cdc45 to Mcm2-7. Dpb11, while bound to Cdc45-Mcm2-7, can block the interaction between GINS and Mcm2-7. Upon the extrusion of ssDNA from the central channel of Mcm2-7, Dpb11 dissociates from Mcm2-7 and Dpb11 binds to ssDNA, thereby allowing GINS to bind to Cdc45-Mcm2-7. Finally, we propose that Dpb11 functions with Sld2 and Sld3 to help control the assembly of the replication fork helicase. Advisors/Committee Members: Dr. Todd Graham (Committee Chair).

Subjects/Keywords: initiation; replication fork; kinase; helicase; replication

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APA (6^th Edition):

Dhingra, N. (2015). Elucidating the role of Dpb11 in replication initiation. (Doctoral Dissertation). Vanderbilt University. Retrieved from http://hdl.handle.net/1803/12863

Chicago Manual of Style (16^th Edition):

Dhingra, Nalini. “Elucidating the role of Dpb11 in replication initiation.” 2015. Doctoral Dissertation, Vanderbilt University. Accessed March 01, 2021. http://hdl.handle.net/1803/12863.

MLA Handbook (7^th Edition):

Dhingra, Nalini. “Elucidating the role of Dpb11 in replication initiation.” 2015. Web. 01 Mar 2021.

Vancouver:

Dhingra N. Elucidating the role of Dpb11 in replication initiation. [Internet] [Doctoral dissertation]. Vanderbilt University; 2015. [cited 2021 Mar 01]. Available from: http://hdl.handle.net/1803/12863.

Council of Science Editors:

Dhingra N. Elucidating the role of Dpb11 in replication initiation. [Doctoral Dissertation]. Vanderbilt University; 2015. Available from: http://hdl.handle.net/1803/12863

Queensland University of Technology

5. Williams, Brett Robert. Development of a novel rep-inducible tomato leaf curl virus expression system.

Degree: 2007, Queensland University of Technology

URL: https://eprints.qut.edu.au/16539/

► Pathogen-derived resistance (PDR) strategies, particularly those based on post-transcriptional gene silencing, have been used with great success for the generation of transgenic plants with resistance… (more)

▼ Pathogen-derived resistance (PDR) strategies, particularly those based on post-transcriptional gene silencing, have been used with great success for the generation of transgenic plants with resistance to RNA viruses. In contrast, a suitable strategy for transgenic resistance to ssDNA plant viruses, including those viruses belonging to the Geminiviridae, has remained elusive. Further, there is no convincing evidence that either post-transcriptional gene silencing, or pathogen-derived resistance in general, would be broadly applicable to ssDNA plant viruses. Researchers at QUT have been developing a novel resistance strategy against ssDNA viruses based on virus-activated expression of a stably integrated suicide gene. The strategy, based on InPAct (In Plant Activation) technology, relies on a "split" suicide gene cassette being arranged in such a way that expression of a lethal ribonuclease (barnase) is dependent on the virus-encoded replication-associated protein (Rep). Upon infection, Rep mediates the release of the construct resulting in the reconstitution of a transcribable and translatable episomal suicide gene expression cassette. The research for this PhD describes the development of an InPAct vector designed to confer resistance to Tomato leaf curl begomovirus (ToLCV), a major cause of disease in Solanaceous crops in the tropics and subtropics. ToLCV-based InPAct vectors were constructed based upon two ToLCV isolates from Australia and North Vietnam. Prior to the generation of InPAct cassettes, the entire ToLCV-[Au] and ToLCV-Vie intergenic regions (IRs) were embedded within the castorbean catalase intron of a β-glucuronidase expression vector to determine the effect of the IR upon transcript processing. Using transient reporter gene assays in tobacco NT-1 cells, it was demonstrated that the ToLCV IRs both contained cryptic intron splice sites which interfered with efficient transcript processing and GUS expression. A series of truncations to the IRs were subsequently made to identify the potential cryptic intron splice sites and/or interfering sequences in both the ToLCV-[Au] and ToLCV-Vie IRs. The final truncated IRs, which were used in the construction the InPAct cassettes, comprised approximately 100 bp and appeared to contain all the necessary cis-acting elements required for efficient rolling circle replication (RCR). Using histochemical GUS assays and Southern analyses, the InPAct cassettes were shown to be activated and replicated only in the presence of the cognate viral Rep. GUS expression levels were shown to be further enhanced in the presence of the ToLCV replication-enhancer protein (REn) and by the addition of the Tobacco yellow dwarf mastrevirus origin of second strand synthesis into the cassette. Under these conditions, Rep-activated GUS expression from the InPAct vectors was found to reach levels similar to that of the benchmark CaMV 35S promoter. Fifteen independent transgenic lines containing the ToLCV-[Au] and -Vie InPAct-GUS cassettes were generated by…

Subjects/Keywords: pathogen-derived resistance (PDR); tomato leaf curl virus; replication-association protein (Rep); begomoviruses

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APA (6^th Edition):

Williams, B. R. (2007). Development of a novel rep-inducible tomato leaf curl virus expression system. (Thesis). Queensland University of Technology. Retrieved from https://eprints.qut.edu.au/16539/

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Williams, Brett Robert. “Development of a novel rep-inducible tomato leaf curl virus expression system.” 2007. Thesis, Queensland University of Technology. Accessed March 01, 2021. https://eprints.qut.edu.au/16539/.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Williams, Brett Robert. “Development of a novel rep-inducible tomato leaf curl virus expression system.” 2007. Web. 01 Mar 2021.

Vancouver:

Williams BR. Development of a novel rep-inducible tomato leaf curl virus expression system. [Internet] [Thesis]. Queensland University of Technology; 2007. [cited 2021 Mar 01]. Available from: https://eprints.qut.edu.au/16539/.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Williams BR. Development of a novel rep-inducible tomato leaf curl virus expression system. [Thesis]. Queensland University of Technology; 2007. Available from: https://eprints.qut.edu.au/16539/

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Queensland University of Technology

6. Pirlo, Steven Dominic. Identification of viral-based replicating vectors suitable for the development of a sugarcane bioreactor.

Degree: 2007, Queensland University of Technology

URL: https://eprints.qut.edu.au/16548/

► The circular, single-stranded (ss) DNA genomes of plant viruses in the families Geminiviridae and Nanoviridae are replicated within the nucleus of a host cell by… (more)

▼ The circular, single-stranded (ss) DNA genomes of plant viruses in the families Geminiviridae and Nanoviridae are replicated within the nucleus of a host cell by a mechanism called rolling circle replication (RCR). Although this process relies almost exclusively on the replication machinery of the host cell, initiation occurs via the interaction of the viral replication initiation protein (Rep) with regulatory DNA sequences within the viral genome. The use of a virus-based episomal amplification technology as a plant bioreactor platform exploits the process of Rep-mediated RCR for the high-level amplification of virus-based episomes in plants and subsequent expression of heterologous proteins; such an approach offers advantages over existing gene expression technologies. This PhD thesis describes research towards the development of a virus-based episomal amplification system for use in sugarcane. Such a crop is ideally suited for a plant bioreactor system due to the efficient high-level production of plant biomass and the existence of established production, harvesting and processing infrastructure. In order to rapidly assess the potential of a virus-based episomal amplification system in sugarcane, a transient assay system was established. Sugarcane callus was identified as the most suitable cell preparation; providing rapid cell regeneration, uniform experimental samples and upon isolation, total DNA suitable for Southern analysis. This assay system once established, proved effective in rapidly identifying virus-based episomes capable of undergoing RCR within sugarcane host cells. This transient assay system was then used to test the functionality of a virus-based episomal amplification system based on the ssDNA virus, Banana bunchy top virus (BBTV) in sugarcane. BBTV-based episomal amplification vectors were constructed with a reporter gene expression cassette flanked by two copies of the BBTV regulatory DNA sequences. The episomal amplification vectors were bombarded into sugarcane and banana host cells in various combinations and evidence of RCR was assessed through Southern blot analysis. RCR products were identified in banana host cells bombarded with the BBTV-based episomal amplification vectors in combination with vectors encoding BBTV Master-Rep (M Rep). RCR products were not identified within sugarcane cells bombarded with the same construct combinations. Integrated InPAct (In Plant Activation) episomal vectors based on BBTV were then employed to confirm the transient results, in addition, the functionality of an InPAct vector based on an alternate virus, Tobacco yellow dwarf virus (TYDV) was also assessed. InPAct vectors based on BBTV were constructed with an untranslatable expression cassette for integration within the sugarcane genome. Transient experiments were performed to assess the ability of BBTV M-Rep and TYDV Rep to initiate RCR of their respective InPAct vectors. Visual observation of GFP expression indicated that BBTV M-Rep was capable of initiating RCR of the BBTVbased InPAct vectors…

Subjects/Keywords: bioreactor; episomal; amplification; rolling circle replication; replication initiation protein; replication accessory protein; TYDV; BBTV; ToLCV-Au; CSMV; ssDNA plant virus

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APA (6^th Edition):

Pirlo, S. D. (2007). Identification of viral-based replicating vectors suitable for the development of a sugarcane bioreactor. (Thesis). Queensland University of Technology. Retrieved from https://eprints.qut.edu.au/16548/

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Pirlo, Steven Dominic. “Identification of viral-based replicating vectors suitable for the development of a sugarcane bioreactor.” 2007. Thesis, Queensland University of Technology. Accessed March 01, 2021. https://eprints.qut.edu.au/16548/.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Pirlo, Steven Dominic. “Identification of viral-based replicating vectors suitable for the development of a sugarcane bioreactor.” 2007. Web. 01 Mar 2021.

Vancouver:

Pirlo SD. Identification of viral-based replicating vectors suitable for the development of a sugarcane bioreactor. [Internet] [Thesis]. Queensland University of Technology; 2007. [cited 2021 Mar 01]. Available from: https://eprints.qut.edu.au/16548/.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Pirlo SD. Identification of viral-based replicating vectors suitable for the development of a sugarcane bioreactor. [Thesis]. Queensland University of Technology; 2007. Available from: https://eprints.qut.edu.au/16548/

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Temple University

7. Cutrera, Jason Lewis. Insights into the Structure and Function of PrgW and its Conserved Cysteines.

Degree: PhD, 2014, Temple University

URL: http://digital.library.temple.edu/u?/p245801coll10,299645

►

Microbiology and Immunology

Enterococcus faecalis is a Gram-positive bacterial species that is typically a member of the human gastrointestinal tract microbiota. However, E. faecalis is… (more)

▼

Microbiology and Immunology

Enterococcus faecalis is a Gram-positive bacterial species that is typically a member of the human gastrointestinal tract microbiota. However, E. faecalis is also a nosocomial pathogen, which is involved in urinary tract infections, soft tissue infections and endocarditis. In recent times, the occurrence of antibiotic resistance has complicated the treatment of these infections. One of the major differences between commensal and pathogenic strains of E. faecalis is that pathogens contain multiple mobile elements such as plasmids, transposons and integrative conjugative elements (ICE). These elements allow for the acquisition and transfer of virulence factors and resistance genes. Conjugative plasmids are a class of plasmids present in E. faecalis whose transfer to host cells is induced by a small pheromone peptide, cCF10 (LVTLVFV). This peptide is initially encoded as a 22-amino acid precursor (pre-cCF10) from the signal sequence of the chromosomal ccfA gene and is then proteolytically cleaved by signal peptidase II and Eep. Once pCF10 has been transferred a host E. faecalis cell, it is exceptionally stable. A replicon clone is maintained in greater than 85% of host cells over 100 generations in the absence of selection, suggesting the stability of pCF10 is intrinsic to the replicon. Three unique features of the replication initiation protein PrgW may contribute to this stability: (a) the interaction of PrgW with pre-cCF10, (b) disulfide bond formation at three conserved cysteines (C78, C275, and C307) in PrgW, and (c) processing of the nascent PrgW protein. Replication initiation proteins associated with theta replicons, such as pCF10, are often self-contained units. To initiate plasmid replication, the replication initiation protein (PrgW) binds to direct repeats (oriV) in its own coding sequence (prgW). In silico analysis of PrgW suggests the existence of three distinct domains within the protein. The first 122 amino acids are homologous to a conserved domain present in related replication initiation proteins, which includes a Helix-Turn-Helix (HTH) DNA binding domain. This suggests that this domain of PrgW has a DNA-binding function and binds to the oriV site in prgW. The following 61 amino acids are not similar to any known sequence, and are encoded by the DNA sequence containing the direct repeats in the oriV site. This domain may or may not have a distinct function. The remaining sequence forms a domain that contains cysteines C275 and C307, and is also similar to no known structure. It is hypothesized that this domain is related to the stability of pCF10. C307 appears to be critical, as previous experiments indicate that its mutation alone affects plasmid stability. Secondary structure analysis of this domain revealed the presence of multiple alpha-helices that contain distinct hydrophobic domains that possibly contribute to pre-cCF10 binding and PrgW tertiary structure. The positions of the conserved cysteines within these alpha-helices may stabilize a hydrophobic binding…

Advisors/Committee Members: Buttaro, Bettina A.;, Tsygankov, Alexander, Tukel, Cagla, Monestier, Marc, Grubmeyer, Charles;.

Subjects/Keywords: Microbiology;

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APA (6^th Edition):

Cutrera, J. L. (2014). Insights into the Structure and Function of PrgW and its Conserved Cysteines. (Doctoral Dissertation). Temple University. Retrieved from http://digital.library.temple.edu/u?/p245801coll10,299645

Chicago Manual of Style (16^th Edition):

Cutrera, Jason Lewis. “Insights into the Structure and Function of PrgW and its Conserved Cysteines.” 2014. Doctoral Dissertation, Temple University. Accessed March 01, 2021. http://digital.library.temple.edu/u?/p245801coll10,299645.

MLA Handbook (7^th Edition):

Cutrera, Jason Lewis. “Insights into the Structure and Function of PrgW and its Conserved Cysteines.” 2014. Web. 01 Mar 2021.

Vancouver:

Cutrera JL. Insights into the Structure and Function of PrgW and its Conserved Cysteines. [Internet] [Doctoral dissertation]. Temple University; 2014. [cited 2021 Mar 01]. Available from: http://digital.library.temple.edu/u?/p245801coll10,299645.

Council of Science Editors:

Cutrera JL. Insights into the Structure and Function of PrgW and its Conserved Cysteines. [Doctoral Dissertation]. Temple University; 2014. Available from: http://digital.library.temple.edu/u?/p245801coll10,299645

Universiteit Utrecht

8. Breukelen, B. van. The role of the adenovirus DNA binding protein in DNA replication and recombination.

Degree: 2003, Universiteit Utrecht

URL: http://dspace.library.uu.nl:8080/handle/1874/226

► Replication of adenovirus DNA in infected cells is an efficient process that, compared to cellular replication, has the use of a protein primer as a… (more)

Subjects/Keywords: Biologie; adenovirus; DBP; unwinding; replication; recombination; initiation; elongation; protein-interaction; DNA

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APA (6^th Edition):

Breukelen, B. v. (2003). The role of the adenovirus DNA binding protein in DNA replication and recombination. (Doctoral Dissertation). Universiteit Utrecht. Retrieved from http://dspace.library.uu.nl:8080/handle/1874/226

Chicago Manual of Style (16^th Edition):

Breukelen, B van. “The role of the adenovirus DNA binding protein in DNA replication and recombination.” 2003. Doctoral Dissertation, Universiteit Utrecht. Accessed March 01, 2021. http://dspace.library.uu.nl:8080/handle/1874/226.

MLA Handbook (7^th Edition):

Breukelen, B van. “The role of the adenovirus DNA binding protein in DNA replication and recombination.” 2003. Web. 01 Mar 2021.

Vancouver:

Breukelen Bv. The role of the adenovirus DNA binding protein in DNA replication and recombination. [Internet] [Doctoral dissertation]. Universiteit Utrecht; 2003. [cited 2021 Mar 01]. Available from: http://dspace.library.uu.nl:8080/handle/1874/226.

Council of Science Editors:

Breukelen Bv. The role of the adenovirus DNA binding protein in DNA replication and recombination. [Doctoral Dissertation]. Universiteit Utrecht; 2003. Available from: http://dspace.library.uu.nl:8080/handle/1874/226

University of Saskatchewan

9. Horbay, Monique Adelle. Inhibition phenotype specific for orië replication-dependent phage growth, and a reappraisal of the Influence of ë P expression on escherichia coli cell metabolism : p-interference phenotype.

Degree: 2005, University of Saskatchewan

URL: http://hdl.handle.net/10388/etd-12222005-145257

► Bacteriophage ë has been used as a model replicon system for forty years. While the basic ë replication initiation scheme has been elucidated for several… (more)

▼ Bacteriophage ë has been used as a model replicon system for forty years. While the basic ë replication initiation scheme has been elucidated for several decades, many aspects of the mechanisms are unclear. I wished to study two unanswered issues in ë replication initiation. Replication initiation of E. coli and ë each depend upon a protein generally called a licensing factor, which brings the DnaB helicase protein to the origin site to begin DNA synthesis. The licensing factors are the products of host gene dnaC and ë gene P. The synthesis of P from ë DNA in an E. coli cell can competitively interfere with DnaC activity needed for E. coli replication initiation. I wished to learn more about what happens to a host cell when exposed to extended P expression. Previous studies in this laboratory suggested that i) the continuous expression of P was tolerated by a subset of exposed cells and that ii) host defects mapping to dnaB could suppress the effect of extended P expression (P-lethality). I used DNA sequencing to determine if these suppressor mutations were within dnaB. I screened known host mutations for their influence on P-lethality. In summary: E. coli strains with GrpD55 and GrpA80 defects were found to each have two point mutations within their dnaB genes. I was unable to isolate mutations within P that suppressed P-lethality and instead obtained regulatory mutations preventing wild type P expression. Two of these sequenced mutations showed that a cI[Ts] lambda repressor was reverted to cI wild type, blocking P expression at all assay temperatures. P-lethality was reversible in cells exposed to P for up to five hours, causing me to suggest that P-Interference be used in place of the term P-lethality. A non-inducible allele of lexA prevented P-mediated cellular filamentation and enhanced P-Interference. This suggests that induction of the SOS response helps cells to tolerate extended P expression. A host strain containing a defective ClpXP protease significantly enhanced cellular sensitivity to P-Interference. This suggests an important role for the ClpXP chaperone-protease complex in degradation of P and cellular resistance to P expression. I present models to explain the P-Interference Phenotype.Recent reports have re-opened the possibility that the tO-oop-pO element influences ë DNA replication initiation. I have also been investigating this possibility. I found that a plasmid with tO-oop-pO (the terminator, nucleotide sequence and promoter for OOP RNA) and orië DNA sequence was inhibitory to the development of repë phages, and designated this the Inhibition Phenotype (IP). In pursuing the mechanism for this inhibition, I mutated the tO-oop-pO and orië elements. I found that the expression of the 77nt OOP RNA transcript and the presence of four 18 base pair repeats (iterons) within orië were required for the IP. I isolated spontaneous phage mutants, resistant to the IP. I determined that singly infected cells were sensitive to the IP but that multiply infected cells escaped the IP. I… Advisors/Committee Members: Hayes, Sidney, Xiao, Wei, Loh, Lambert, Howard, S. Peter, Goldie, Hughes, Cupples, Claire, Bull, Harold.

Subjects/Keywords: OOP RNA; DNA replication initiation; lambda P protein; bacteriophage lambda

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APA (6^th Edition):

Horbay, M. A. (2005). Inhibition phenotype specific for orië replication-dependent phage growth, and a reappraisal of the Influence of ë P expression on escherichia coli cell metabolism : p-interference phenotype. (Thesis). University of Saskatchewan. Retrieved from http://hdl.handle.net/10388/etd-12222005-145257

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Horbay, Monique Adelle. “Inhibition phenotype specific for orië replication-dependent phage growth, and a reappraisal of the Influence of ë P expression on escherichia coli cell metabolism : p-interference phenotype.” 2005. Thesis, University of Saskatchewan. Accessed March 01, 2021. http://hdl.handle.net/10388/etd-12222005-145257.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Horbay, Monique Adelle. “Inhibition phenotype specific for orië replication-dependent phage growth, and a reappraisal of the Influence of ë P expression on escherichia coli cell metabolism : p-interference phenotype.” 2005. Web. 01 Mar 2021.

Vancouver:

Horbay MA. Inhibition phenotype specific for orië replication-dependent phage growth, and a reappraisal of the Influence of ë P expression on escherichia coli cell metabolism : p-interference phenotype. [Internet] [Thesis]. University of Saskatchewan; 2005. [cited 2021 Mar 01]. Available from: http://hdl.handle.net/10388/etd-12222005-145257.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Horbay MA. Inhibition phenotype specific for orië replication-dependent phage growth, and a reappraisal of the Influence of ë P expression on escherichia coli cell metabolism : p-interference phenotype. [Thesis]. University of Saskatchewan; 2005. Available from: http://hdl.handle.net/10388/etd-12222005-145257

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Gothenburg / Göteborgs Universitet

10. Simonsson, Stina 1969-. Initiation of herpes simplex virus DNA replication.

Degree: 2000, University of Gothenburg / Göteborgs Universitet

URL: http://hdl.handle.net/2077/14135

► During virus infection multiple new copies of the virus genome are made by a DNA synthesis machinery. Initiation of DNA replication involves the recognition of… (more)

Subjects/Keywords: initiation of DNA replication; origin binding protein; Herpes Simplex Virus

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APA (6^th Edition):

Simonsson, S. 1. (2000). Initiation of herpes simplex virus DNA replication. (Thesis). University of Gothenburg / Göteborgs Universitet. Retrieved from http://hdl.handle.net/2077/14135

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Simonsson, Stina 1969-. “Initiation of herpes simplex virus DNA replication.” 2000. Thesis, University of Gothenburg / Göteborgs Universitet. Accessed March 01, 2021. http://hdl.handle.net/2077/14135.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Simonsson, Stina 1969-. “Initiation of herpes simplex virus DNA replication.” 2000. Web. 01 Mar 2021.

Vancouver:

Simonsson S1. Initiation of herpes simplex virus DNA replication. [Internet] [Thesis]. University of Gothenburg / Göteborgs Universitet; 2000. [cited 2021 Mar 01]. Available from: http://hdl.handle.net/2077/14135.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Simonsson S1. Initiation of herpes simplex virus DNA replication. [Thesis]. University of Gothenburg / Göteborgs Universitet; 2000. Available from: http://hdl.handle.net/2077/14135

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

11. Brenkman, A.B. (Arjan Bernard). Molecular architecture and function of adenovirus DNA polymerase.

Degree: 2002, University Utrecht

URL: https://dspace.library.uu.nl/handle/1874/462 ; URN:NBN:NL:UI:10-1874-462 ; URN:NBN:NL:UI:10-1874-462 ; https://dspace.library.uu.nl/handle/1874/462

► Central to this thesis is the role of adenovirus DNA polymerase (Ad pol) in adenovirus DNA replication. Ad pol is a member of the family… (more)

Subjects/Keywords: protein priming; DNA polymerase; replication; NFI; transcription factor; adenovirus; termination; initiation; pTP; origin

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Brenkman, A. B. (. B. (2002). Molecular architecture and function of adenovirus DNA polymerase. (Doctoral Dissertation). University Utrecht. Retrieved from https://dspace.library.uu.nl/handle/1874/462 ; URN:NBN:NL:UI:10-1874-462 ; URN:NBN:NL:UI:10-1874-462 ; https://dspace.library.uu.nl/handle/1874/462

Chicago Manual of Style (16^th Edition):

Brenkman, A B (Arjan Bernard). “Molecular architecture and function of adenovirus DNA polymerase.” 2002. Doctoral Dissertation, University Utrecht. Accessed March 01, 2021. https://dspace.library.uu.nl/handle/1874/462 ; URN:NBN:NL:UI:10-1874-462 ; URN:NBN:NL:UI:10-1874-462 ; https://dspace.library.uu.nl/handle/1874/462.

MLA Handbook (7^th Edition):

Brenkman, A B (Arjan Bernard). “Molecular architecture and function of adenovirus DNA polymerase.” 2002. Web. 01 Mar 2021.

Vancouver:

Brenkman AB(B. Molecular architecture and function of adenovirus DNA polymerase. [Internet] [Doctoral dissertation]. University Utrecht; 2002. [cited 2021 Mar 01]. Available from: https://dspace.library.uu.nl/handle/1874/462 ; URN:NBN:NL:UI:10-1874-462 ; URN:NBN:NL:UI:10-1874-462 ; https://dspace.library.uu.nl/handle/1874/462.

Council of Science Editors:

Brenkman AB(B. Molecular architecture and function of adenovirus DNA polymerase. [Doctoral Dissertation]. University Utrecht; 2002. Available from: https://dspace.library.uu.nl/handle/1874/462 ; URN:NBN:NL:UI:10-1874-462 ; URN:NBN:NL:UI:10-1874-462 ; https://dspace.library.uu.nl/handle/1874/462

12. Breukelen, B. van. The role of the adenovirus DNA binding protein in DNA replication and recombination.

Degree: 2003, University Utrecht

URL: https://dspace.library.uu.nl/handle/1874/226 ; URN:NBN:NL:UI:10-1874-226 ; 1874/226 ; URN:NBN:NL:UI:10-1874-226 ; https://dspace.library.uu.nl/handle/1874/226

► Replication of adenovirus DNA in infected cells is an efficient process that, compared to cellular replication, has the use of a protein primer as a… (more)

Subjects/Keywords: adenovirus; DBP; unwinding; replication; recombination; initiation; elongation; protein-interaction; DNA

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Breukelen, B. v. (2003). The role of the adenovirus DNA binding protein in DNA replication and recombination. (Doctoral Dissertation). University Utrecht. Retrieved from https://dspace.library.uu.nl/handle/1874/226 ; URN:NBN:NL:UI:10-1874-226 ; 1874/226 ; URN:NBN:NL:UI:10-1874-226 ; https://dspace.library.uu.nl/handle/1874/226

Chicago Manual of Style (16^th Edition):

Breukelen, B van. “The role of the adenovirus DNA binding protein in DNA replication and recombination.” 2003. Doctoral Dissertation, University Utrecht. Accessed March 01, 2021. https://dspace.library.uu.nl/handle/1874/226 ; URN:NBN:NL:UI:10-1874-226 ; 1874/226 ; URN:NBN:NL:UI:10-1874-226 ; https://dspace.library.uu.nl/handle/1874/226.

MLA Handbook (7^th Edition):

Breukelen, B van. “The role of the adenovirus DNA binding protein in DNA replication and recombination.” 2003. Web. 01 Mar 2021.

Vancouver:

Breukelen Bv. The role of the adenovirus DNA binding protein in DNA replication and recombination. [Internet] [Doctoral dissertation]. University Utrecht; 2003. [cited 2021 Mar 01]. Available from: https://dspace.library.uu.nl/handle/1874/226 ; URN:NBN:NL:UI:10-1874-226 ; 1874/226 ; URN:NBN:NL:UI:10-1874-226 ; https://dspace.library.uu.nl/handle/1874/226.

Council of Science Editors:

Breukelen Bv. The role of the adenovirus DNA binding protein in DNA replication and recombination. [Doctoral Dissertation]. University Utrecht; 2003. Available from: https://dspace.library.uu.nl/handle/1874/226 ; URN:NBN:NL:UI:10-1874-226 ; 1874/226 ; URN:NBN:NL:UI:10-1874-226 ; https://dspace.library.uu.nl/handle/1874/226

13. Brenkman, A.B. (Arjan Bernard). Molecular architecture and function of adenovirus DNA polymerase.

Degree: 2002, University Utrecht

URL: https://dspace.library.uu.nl/handle/1874/462 ; URN:NBN:NL:UI:10-1874-462 ; 1874/462 ; URN:NBN:NL:UI:10-1874-462 ; https://dspace.library.uu.nl/handle/1874/462

► Central to this thesis is the role of adenovirus DNA polymerase (Ad pol) in adenovirus DNA replication. Ad pol is a member of the family… (more)

Subjects/Keywords: protein priming; DNA polymerase; replication; NFI; transcription factor; adenovirus; termination; initiation; pTP; origin

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Brenkman, A. B. (. B. (2002). Molecular architecture and function of adenovirus DNA polymerase. (Doctoral Dissertation). University Utrecht. Retrieved from https://dspace.library.uu.nl/handle/1874/462 ; URN:NBN:NL:UI:10-1874-462 ; 1874/462 ; URN:NBN:NL:UI:10-1874-462 ; https://dspace.library.uu.nl/handle/1874/462

Chicago Manual of Style (16^th Edition):

Brenkman, A B (Arjan Bernard). “Molecular architecture and function of adenovirus DNA polymerase.” 2002. Doctoral Dissertation, University Utrecht. Accessed March 01, 2021. https://dspace.library.uu.nl/handle/1874/462 ; URN:NBN:NL:UI:10-1874-462 ; 1874/462 ; URN:NBN:NL:UI:10-1874-462 ; https://dspace.library.uu.nl/handle/1874/462.

MLA Handbook (7^th Edition):

Brenkman, A B (Arjan Bernard). “Molecular architecture and function of adenovirus DNA polymerase.” 2002. Web. 01 Mar 2021.

Vancouver:

Brenkman AB(B. Molecular architecture and function of adenovirus DNA polymerase. [Internet] [Doctoral dissertation]. University Utrecht; 2002. [cited 2021 Mar 01]. Available from: https://dspace.library.uu.nl/handle/1874/462 ; URN:NBN:NL:UI:10-1874-462 ; 1874/462 ; URN:NBN:NL:UI:10-1874-462 ; https://dspace.library.uu.nl/handle/1874/462.

Council of Science Editors:

Brenkman AB(B. Molecular architecture and function of adenovirus DNA polymerase. [Doctoral Dissertation]. University Utrecht; 2002. Available from: https://dspace.library.uu.nl/handle/1874/462 ; URN:NBN:NL:UI:10-1874-462 ; 1874/462 ; URN:NBN:NL:UI:10-1874-462 ; https://dspace.library.uu.nl/handle/1874/462

Queensland University of Technology

14. Hastie, Marcus Lachlan. In vitro activities of BBTV-REP.

Degree: 2001, Queensland University of Technology

URL: http://eprints.qut.edu.au/37077/

Subjects/Keywords: Banana bunchy top disease; Bananas Diseases and pests; REP; replication associated protein; replication initiator protein; banana bunchy top virus; nanovirus; geminivirus; rolling circle replication; topoisomerase; ATPase; thesis; doctoral

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Hastie, M. L. (2001). In vitro activities of BBTV-REP. (Thesis). Queensland University of Technology. Retrieved from http://eprints.qut.edu.au/37077/

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Hastie, Marcus Lachlan. “In vitro activities of BBTV-REP.” 2001. Thesis, Queensland University of Technology. Accessed March 01, 2021. http://eprints.qut.edu.au/37077/.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Hastie, Marcus Lachlan. “In vitro activities of BBTV-REP.” 2001. Web. 01 Mar 2021.

Vancouver:

Hastie ML. In vitro activities of BBTV-REP. [Internet] [Thesis]. Queensland University of Technology; 2001. [cited 2021 Mar 01]. Available from: http://eprints.qut.edu.au/37077/.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Hastie ML. In vitro activities of BBTV-REP. [Thesis]. Queensland University of Technology; 2001. Available from: http://eprints.qut.edu.au/37077/

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Hong Kong University of Science and Technology

15. Huang, Yining. The role of hIpi3 in human DNA replication.

Degree: 2013, Hong Kong University of Science and Technology

URL: http://repository.ust.hk/ir/Record/1783.1-62314 ; https://doi.org/10.14711/thesis-b1254380 ; http://repository.ust.hk/ir/bitstream/1783.1-62314/1/th_redirect.html

► Ipi3 is required for cell viability in Saccharomyces cerevisiae. Yeast Ipi3 has been reported to be an essential component of the Rix1 complex (Rix1-Ipi1-Ipi3) that… (more)

Subjects/Keywords: DNA replication ; DNA-protein interactions

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APA (6^th Edition):

Huang, Y. (2013). The role of hIpi3 in human DNA replication. (Thesis). Hong Kong University of Science and Technology. Retrieved from http://repository.ust.hk/ir/Record/1783.1-62314 ; https://doi.org/10.14711/thesis-b1254380 ; http://repository.ust.hk/ir/bitstream/1783.1-62314/1/th_redirect.html

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Huang, Yining. “The role of hIpi3 in human DNA replication.” 2013. Thesis, Hong Kong University of Science and Technology. Accessed March 01, 2021. http://repository.ust.hk/ir/Record/1783.1-62314 ; https://doi.org/10.14711/thesis-b1254380 ; http://repository.ust.hk/ir/bitstream/1783.1-62314/1/th_redirect.html.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Huang, Yining. “The role of hIpi3 in human DNA replication.” 2013. Web. 01 Mar 2021.

Vancouver:

Huang Y. The role of hIpi3 in human DNA replication. [Internet] [Thesis]. Hong Kong University of Science and Technology; 2013. [cited 2021 Mar 01]. Available from: http://repository.ust.hk/ir/Record/1783.1-62314 ; https://doi.org/10.14711/thesis-b1254380 ; http://repository.ust.hk/ir/bitstream/1783.1-62314/1/th_redirect.html.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Huang Y. The role of hIpi3 in human DNA replication. [Thesis]. Hong Kong University of Science and Technology; 2013. Available from: http://repository.ust.hk/ir/Record/1783.1-62314 ; https://doi.org/10.14711/thesis-b1254380 ; http://repository.ust.hk/ir/bitstream/1783.1-62314/1/th_redirect.html

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Université Paris-Sud – Paris XI

16. Saïfi, Boubekeur. Caractérisation de cycC, un nouveau gène impliqué dans le programme de réplication d'Escherichia coli : Characterization of cycC, a new gene involved in the replication program of Escherichia coli.

Degree: Docteur es, Aspects moléculaires et cellulaires de la biologie, 2012, Université Paris-Sud – Paris XI

URL: http://www.theses.fr/2012PA112211

►

Dans Escherichia coli la Dam Methyl Transferase (DamMT) est responsable du transfert d’un groupement méthyle sur les adénosines situés au cœur du tétranucléotide GATC; il… (more)

▼

Dans Escherichia coli la Dam Methyl Transferase (DamMT) est responsable du transfert d’un groupement méthyle sur les adénosines situés au cœur du tétranucléotide GATC; il s’agit donc d’une activité post réplicative. Ainsi, après le passage de la fourche de réplication, le brin d’ADN nouvellement synthétisé est non méthylé – l’ADN est dit hémimethylé. L’ADN reste hémimethylé pendent une brève période - de l’ordre de la minute - avant d’être reméthylé par la DamMT. L’hypothèse de l’implication de la méthylation de l’ADN dans le contrôle général du programme de maintenance de l’ADN repose essentiellement sur cette observation, puisque l’ADN hemimethyle – exception faite de l’origine de réplication et de la région promotrice du gène dnaA – est diagnostique du passage récent de la fourche de réplication. Cette hypothèse, et le criblage phylogénomique qui en a découlé a conduit a l’identification de plusieurs gènes dont les produits sont supposes être impliqués dans la maintenance de l’ADN. yjaG est l’un de ces gènes. Il a été renomme cycC en raison des dérèglements de la progression du cycle cellulaire associés a un mutant nul de ce gène. L’étude effectuée au cours de ma thèse s’attachera à expliquer l’état actuel de nos connaissances sur la protéine CycC et de son implication dans le processus de réplication de l’ADN. Nos résultats montrent que la protéine CycC est impliquée dans la processivité de la réplication lorsqu’il y a un dommage au niveau de l’ADN. CycC spécifie une activité qui conduit à freiner les fourches de réplication, afin de prévenir des avortements des réplisomes. La surexpression de CycC bloque l’initiation de la réplication entre l’ouverture de la molécule d’ADN et le chargement de l’hélicase réplicative. Nous proposons que CycC interagisse avec le complexe réplicative et ralentit les fourches de réplication. Ce ralentissement prévient de nouvelles collisions lorsque les cellules sont dans des conditions de stress-qui cause des arrêts de la réplication.

In Escherichia coli the Dam Methyl Transferase (DamMT) is responsible for the transfer of a methyl group on the adenosine located in tetranucleotide GATC, so this is a post-replicative activity. Thus, after the passage of the replication fork, the newly synthesized DNA strand is unmethylated - DNA is called hemimethylated. DNA remains hemimethylated in a brief period - about a minute - before being reméthylé by DamMT. The hypothesis of the involvement of DNA methylation in the general control of the maintenance program of the DNA is essentially on this observation, since the hemimethylated DNA - except the origin of replication and the region dnaA gene promoter - is diagnostic of the recent passage of the replication fork. This assumption and phylogenomics screening has led to the identification of several genes whose protein are supposed to be involved in the maintenance of DNA. yjaG is one of these genes. It was renamed cycC, the cell cycle progression is deregulated with a null mutant of this gene. The study in my thesis will focus on explaining…

Advisors/Committee Members: Ferat, Jean-Luc (thesis director).

Subjects/Keywords: Initiation de la réplication; Réactivation des fourches; Processivité de la réplication; Initiation of replication; Forks reactivation; Processivity of replication

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Saïfi, B. (2012). Caractérisation de cycC, un nouveau gène impliqué dans le programme de réplication d'Escherichia coli : Characterization of cycC, a new gene involved in the replication program of Escherichia coli. (Doctoral Dissertation). Université Paris-Sud – Paris XI. Retrieved from http://www.theses.fr/2012PA112211

Chicago Manual of Style (16^th Edition):

Saïfi, Boubekeur. “Caractérisation de cycC, un nouveau gène impliqué dans le programme de réplication d'Escherichia coli : Characterization of cycC, a new gene involved in the replication program of Escherichia coli.” 2012. Doctoral Dissertation, Université Paris-Sud – Paris XI. Accessed March 01, 2021. http://www.theses.fr/2012PA112211.

MLA Handbook (7^th Edition):

Saïfi, Boubekeur. “Caractérisation de cycC, un nouveau gène impliqué dans le programme de réplication d'Escherichia coli : Characterization of cycC, a new gene involved in the replication program of Escherichia coli.” 2012. Web. 01 Mar 2021.

Vancouver:

Saïfi B. Caractérisation de cycC, un nouveau gène impliqué dans le programme de réplication d'Escherichia coli : Characterization of cycC, a new gene involved in the replication program of Escherichia coli. [Internet] [Doctoral dissertation]. Université Paris-Sud – Paris XI; 2012. [cited 2021 Mar 01]. Available from: http://www.theses.fr/2012PA112211.

Council of Science Editors:

Saïfi B. Caractérisation de cycC, un nouveau gène impliqué dans le programme de réplication d'Escherichia coli : Characterization of cycC, a new gene involved in the replication program of Escherichia coli. [Doctoral Dissertation]. Université Paris-Sud – Paris XI; 2012. Available from: http://www.theses.fr/2012PA112211

University of Minnesota

17. Lovendahl, Klaus. Adaptation of HUH endonucleases for protein-DNA conjugation.

Degree: PhD, Biochemistry, Molecular Bio, and Biophysics, 2018, University of Minnesota

URL: http://hdl.handle.net/11299/201685

► The conjugation of proteins to DNA is a useful technique for basic research and medicine. Current methods for conjugation are limited in both specificity and… (more)

Subjects/Keywords: dna barcoding; huh; magnetic tweezers; protein-dna conjugation; relaxase; rep; replicase

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APA (6^th Edition):

Lovendahl, K. (2018). Adaptation of HUH endonucleases for protein-DNA conjugation. (Doctoral Dissertation). University of Minnesota. Retrieved from http://hdl.handle.net/11299/201685

Chicago Manual of Style (16^th Edition):

Lovendahl, Klaus. “Adaptation of HUH endonucleases for protein-DNA conjugation.” 2018. Doctoral Dissertation, University of Minnesota. Accessed March 01, 2021. http://hdl.handle.net/11299/201685.

MLA Handbook (7^th Edition):

Lovendahl, Klaus. “Adaptation of HUH endonucleases for protein-DNA conjugation.” 2018. Web. 01 Mar 2021.

Vancouver:

Lovendahl K. Adaptation of HUH endonucleases for protein-DNA conjugation. [Internet] [Doctoral dissertation]. University of Minnesota; 2018. [cited 2021 Mar 01]. Available from: http://hdl.handle.net/11299/201685.

Council of Science Editors:

Lovendahl K. Adaptation of HUH endonucleases for protein-DNA conjugation. [Doctoral Dissertation]. University of Minnesota; 2018. Available from: http://hdl.handle.net/11299/201685

University of California – San Diego

18. Li, Dongyang. DNA replication and cell size control in Escherichia coli.

Degree: Biology, 2018, University of California – San Diego

URL: http://www.escholarship.org/uc/item/5tt038j7

► The deﬁning feature of living organisms is their capacity to reproduce and pass on the genetic information so that their progeny can ﬂourish. For bacteria,… (more)

▼ The deﬁning feature of living organisms is their capacity to reproduce and pass on the genetic information so that their progeny can ﬂourish. For bacteria, reproduction is a feat by itself – Escherichia coli cells cultured in optimal conditions grow rapidly and divide about every 20 minutes. In other words, the cell has to replicate all cellular contents, and be ready to divide evenly into two daughter cells within this 20 minutes. Biosynthesis of new cellular materials, e.g. proteins, nucleic acis, lipids and other metabolites accumulate and roughly doubles after every generation. Notably, the deoxyribonucleic acid (DNA) encodes genetic informationand needs to be duplicated in order to faithfully pass on this information to the progeny. This process of DNA replication in the cell needs to dynamically adapt to ﬂuctuation in growth condition and cellular physiology. Such coordination is controlled at the ﬁrst step of replcation – the initiation of replication. In this thesis, I presented the development of methods for measuring DNA replication duration (replication period), the quantitative relationship between DNA replication and cell size as well as the mechanism of replication initiation. DNA replication measurement laid the foundation of studying the quantitative relationship between cell size and DNA replication. A general growth law was proposed to describe cell size regulation in light of three physiological variables including biosynthesis rate, cell cycle progression and replicaiton initiation. Of the three variables, the mass when cell initiates replication (initiation mass) remains invariant despite a wide spectrum of antibiotics or growth limitation challenge. This invariant initiation mass called into question about the mechanism of initiation to achieve such constancy. We proposed a simple threshold model to explain how cells can maintain a invariant initiation mass by regulating the expression of initiation regulators (initiators). The initiaiton mass is inversely proportional to the initiator levels, which is held constant. Experimental evidence was provided to test our model prediction.

Subjects/Keywords: Biology; Microbiology; Biophysics; bacterial physiology; cell size; DNA replication; Escherichia coli; replication initiation

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Li, D. (2018). DNA replication and cell size control in Escherichia coli. (Thesis). University of California – San Diego. Retrieved from http://www.escholarship.org/uc/item/5tt038j7

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Li, Dongyang. “DNA replication and cell size control in Escherichia coli.” 2018. Thesis, University of California – San Diego. Accessed March 01, 2021. http://www.escholarship.org/uc/item/5tt038j7.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Li, Dongyang. “DNA replication and cell size control in Escherichia coli.” 2018. Web. 01 Mar 2021.

Vancouver:

Li D. DNA replication and cell size control in Escherichia coli. [Internet] [Thesis]. University of California – San Diego; 2018. [cited 2021 Mar 01]. Available from: http://www.escholarship.org/uc/item/5tt038j7.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Li D. DNA replication and cell size control in Escherichia coli. [Thesis]. University of California – San Diego; 2018. Available from: http://www.escholarship.org/uc/item/5tt038j7

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Cambridge

19. Jatikusumo, Vincentius Aji. Treslin and its role in the assembly of the replicative DNA helicase.

Degree: PhD, 2020, University of Cambridge

URL: https://www.repository.cam.ac.uk/handle/1810/301381

► DNA replication is an essential biochemical process that underlies genetic inheritance. In eukaryotic cells, this process is carried out by a multi-subunit protein complex called… (more)

▼ DNA replication is an essential biochemical process that underlies genetic inheritance. In eukaryotic cells, this process is carried out by a multi-subunit protein complex called replisome. The core of replisome is made up of six-subunit protein ring Mcm2–7, which contains weak DNA helicase activity. In order to fully activate the helicase activity, protein co-activators of Cdc45 and GINS have to be recruited to assemble a replicative DNA helicase called the CMG (Cdc45-Mcm2–7-GINS) complex. The active CMG subsequently unwinds the parental DNA duplex to provide single-stranded DNA templates for replicative DNA polymerases. The precise mechanism for CMG assembly and activation is still poorly understood. Recent studies using model organisms suggested the importance of protein called Sld3/Treslin for CMG assembly. The aim of the project was to understand in detail how human Treslin participates in the assembly of the CMG helicase. The interactions of Treslin with Cdc45, Mcm2–7, and DNA were characterised by a series of biochemical, biophysical, and structural biology experiments. Cdc45–Treslin interaction was shown to be conserved from budding yeast to human, in which it is mediated by a domain called Sld3/Treslin Homology Domain (SHD). However, human Treslin contains an additional Cdc45-binding site—next to the C-terminus of SHD— in an intrinsically disordered region referred to as Treslin ’extension’ (Treslinext). Treslinext was also demonstrated to interact with Mcm2–7 ring and DNA. S-phase Cyclin-Dependent Kinase (S-CDK) appeared to regulate Treslin’s interaction with Cdc45 and Mcm2–7, in which it targets a number of potential S-CDK phosphorylation sites present in Treslinext. As a part of this project, low-resolution Cdc45–Treslin and medium-resolution of MCM single hexamer 3D maps were obtained through single-particle cryo-electron microscopy (cryo-EM) analyses. The results provide molecular basis for Treslin’s role in the assembly of the CMG complex. Treslin acts as a molecular chaperone for Cdc45 recruitment to the DNA-bound Mcm2-7 ring. Treslin’s role during CMG assembly may involve its binding ability to the Mcm2–7 and DNA. Furthermore, the regulatory role of S-CDK in Treslin’s interactions with Cdc45 and Mcm2–7 suggests a link of the CMG assembly control to the cell cycle in human.

Subjects/Keywords: Treslin; DNA replication; CryoEM; Biochemistry; CMG Helicase; MCM; Cdc45; DNA replication initiation; Replisome

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Jatikusumo, V. A. (2020). Treslin and its role in the assembly of the replicative DNA helicase. (Doctoral Dissertation). University of Cambridge. Retrieved from https://www.repository.cam.ac.uk/handle/1810/301381

Chicago Manual of Style (16^th Edition):

Jatikusumo, Vincentius Aji. “Treslin and its role in the assembly of the replicative DNA helicase.” 2020. Doctoral Dissertation, University of Cambridge. Accessed March 01, 2021. https://www.repository.cam.ac.uk/handle/1810/301381.

MLA Handbook (7^th Edition):

Jatikusumo, Vincentius Aji. “Treslin and its role in the assembly of the replicative DNA helicase.” 2020. Web. 01 Mar 2021.

Vancouver:

Jatikusumo VA. Treslin and its role in the assembly of the replicative DNA helicase. [Internet] [Doctoral dissertation]. University of Cambridge; 2020. [cited 2021 Mar 01]. Available from: https://www.repository.cam.ac.uk/handle/1810/301381.

Council of Science Editors:

Jatikusumo VA. Treslin and its role in the assembly of the replicative DNA helicase. [Doctoral Dissertation]. University of Cambridge; 2020. Available from: https://www.repository.cam.ac.uk/handle/1810/301381

University of Cambridge

20. Jatikusumo, Vincentius Aji. Treslin and its role in the assembly of the replicative DNA helicase.

Degree: PhD, 2020, University of Cambridge

URL: https://doi.org/10.17863/CAM.48462 ; https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.801694

► DNA replication is an essential biochemical process that underlies genetic inheritance. In eukaryotic cells, this process is carried out by a multi-subunit protein complex called… (more)

▼ DNA replication is an essential biochemical process that underlies genetic inheritance. In eukaryotic cells, this process is carried out by a multi-subunit protein complex called replisome. The core of replisome is made up of six-subunit protein ring Mcm2–7, which contains weak DNA helicase activity. In order to fully activate the helicase activity, protein co-activators of Cdc45 and GINS have to be recruited to assemble a replicative DNA helicase called the CMG (Cdc45-Mcm2–7-GINS) complex. The active CMG subsequently unwinds the parental DNA duplex to provide single-stranded DNA templates for replicative DNA polymerases. The precise mechanism for CMG assembly and activation is still poorly understood. Recent studies using model organisms suggested the importance of protein called Sld3/Treslin for CMG assembly. The aim of the project was to understand in detail how human Treslin participates in the assembly of the CMG helicase. The interactions of Treslin with Cdc45, Mcm2–7, and DNA were characterised by a series of biochemical, biophysical, and structural biology experiments. Cdc45–Treslin interaction was shown to be conserved from budding yeast to human, in which it is mediated by a domain called Sld3/Treslin Homology Domain (SHD). However, human Treslin contains an additional Cdc45-binding site—next to the C-terminus of SHD— in an intrinsically disordered region referred to as Treslin ’extension’ (Treslinext). Treslinext was also demonstrated to interact with Mcm2–7 ring and DNA. S-phase Cyclin-Dependent Kinase (S-CDK) appeared to regulate Treslin’s interaction with Cdc45 and Mcm2–7, in which it targets a number of potential S-CDK phosphorylation sites present in Treslinext. As a part of this project, low-resolution Cdc45–Treslin and medium-resolution of MCM single hexamer 3D maps were obtained through single-particle cryo-electron microscopy (cryo-EM) analyses. The results provide molecular basis for Treslin’s role in the assembly of the CMG complex. Treslin acts as a molecular chaperone for Cdc45 recruitment to the DNA-bound Mcm2-7 ring. Treslin’s role during CMG assembly may involve its binding ability to the Mcm2–7 and DNA. Furthermore, the regulatory role of S-CDK in Treslin’s interactions with Cdc45 and Mcm2–7 suggests a link of the CMG assembly control to the cell cycle in human.

Subjects/Keywords: Treslin; DNA replication; CryoEM; Biochemistry; CMG Helicase; MCM; Cdc45; DNA replication initiation; Replisome

Record Details Similar Records Cite « Share

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Jatikusumo, V. A. (2020). Treslin and its role in the assembly of the replicative DNA helicase. (Doctoral Dissertation). University of Cambridge. Retrieved from https://doi.org/10.17863/CAM.48462 ; https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.801694

Chicago Manual of Style (16^th Edition):

Jatikusumo, Vincentius Aji. “Treslin and its role in the assembly of the replicative DNA helicase.” 2020. Doctoral Dissertation, University of Cambridge. Accessed March 01, 2021. https://doi.org/10.17863/CAM.48462 ; https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.801694.

MLA Handbook (7^th Edition):

Jatikusumo, Vincentius Aji. “Treslin and its role in the assembly of the replicative DNA helicase.” 2020. Web. 01 Mar 2021.

Vancouver:

Jatikusumo VA. Treslin and its role in the assembly of the replicative DNA helicase. [Internet] [Doctoral dissertation]. University of Cambridge; 2020. [cited 2021 Mar 01]. Available from: https://doi.org/10.17863/CAM.48462 ; https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.801694.

Council of Science Editors:

Jatikusumo VA. Treslin and its role in the assembly of the replicative DNA helicase. [Doctoral Dissertation]. University of Cambridge; 2020. Available from: https://doi.org/10.17863/CAM.48462 ; https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.801694

University of Waterloo

21. Ramer, Matthew. Characterization of the association of Dbf4 and Cdc7 with Mcm2-7 and chromatin in Saccharomyces cerevisiae.

Degree: 2012, University of Waterloo

URL: http://hdl.handle.net/10012/6429

► Initiation of DNA replication requires the action of the Dbf4/Cdc7 kinase complex (DDK) which is also a phosphorylation target of Rad53 kinase in the S-phase… (more)

▼ Initiation of DNA replication requires the action of the Dbf4/Cdc7 kinase complex (DDK) which is also a phosphorylation target of Rad53 kinase in the S-phase checkpoint. DDK is thought to trigger DNA replication by phosphorylating members of the Mcm2-7 complex present at origins of replication. While DDK phosphorylation sites have been identified on Mcm2-7, the contributions made by Dbf4 and Cdc7 to the targeting of the complex have not been established. DDK has also been implicated in the S-phase checkpoint response since it is removed from chromatin in a Rad53-dependent manner. The interaction of Dbf4 and Cdc7 with each of the Mcm2-7 subunits was assessed and showed an interaction between Dbf4 and Mcm2 and Mcm6, while interactions between Cdc7 and Mcm4 and Mcm5 were observed. Mutations in Mcm2 and Mcm4 that disrupt the interactions with Dbf4 or Cdc7 showed modest growth impairment and compromised DNA replication, while simultaneous abrogation of both interactions resulted in lethality. Strains overexpressing Mcm2 or Mcm4 were sensitive to genotoxic agents, while overexpression of Mcm2 in a Mcm4Δ175-333 strain background resulted in a severe growth impairment as well as sensitivity to genotoxic stress. ChIP analysis revealed the possibility of Dbf4/Cdc7 localization to origin flanking regions through most of S-phase, which may redistribute to origins at the time of firing. Fluorescence microscopy of Mcm2 and Dbf4 in S-phase seem to show a punctate pattern of staining, consistent with these factors’ localization to ‘replication factories.’ By using a Dbf4ΔN mutant, the N-motif was shown to be required for the Rad53-mediated removal of Dbf4 from chromatin under checkpoint conditions. Initial optimization of a DNA combing protocol was also performed, which along with Dbf4ΔN mutant and the fluorescently-epitope tagged strains, will be useful tools for evaluating a role for DDK in the S-phase checkpoint response. Altered levels of DNA replication factors have been implicated in many human cancers. The data presented in this study provide novel insight into the normal process of the initiation of DNA replication which can be applied to research involving higher eukaryotes, including humans, and can serve as a benchmark for comparison with the cancer phenotype.

Subjects/Keywords: Cell cycle; Initiation of DNA Replication; S-phase checkpoint

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Ramer, M. (2012). Characterization of the association of Dbf4 and Cdc7 with Mcm2-7 and chromatin in Saccharomyces cerevisiae. (Thesis). University of Waterloo. Retrieved from http://hdl.handle.net/10012/6429

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Ramer, Matthew. “Characterization of the association of Dbf4 and Cdc7 with Mcm2-7 and chromatin in Saccharomyces cerevisiae.” 2012. Thesis, University of Waterloo. Accessed March 01, 2021. http://hdl.handle.net/10012/6429.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Ramer, Matthew. “Characterization of the association of Dbf4 and Cdc7 with Mcm2-7 and chromatin in Saccharomyces cerevisiae.” 2012. Web. 01 Mar 2021.

Vancouver:

Ramer M. Characterization of the association of Dbf4 and Cdc7 with Mcm2-7 and chromatin in Saccharomyces cerevisiae. [Internet] [Thesis]. University of Waterloo; 2012. [cited 2021 Mar 01]. Available from: http://hdl.handle.net/10012/6429.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Ramer M. Characterization of the association of Dbf4 and Cdc7 with Mcm2-7 and chromatin in Saccharomyces cerevisiae. [Thesis]. University of Waterloo; 2012. Available from: http://hdl.handle.net/10012/6429

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Universiteit Utrecht

22. Brenkman, A.B. (Arjan Bernard). Molecular architecture and function of adenovirus DNA polymerase.

Degree: 2002, Universiteit Utrecht

URL: http://dspace.library.uu.nl:8080/handle/1874/462

► Central to this thesis is the role of adenovirus DNA polymerase (Ad pol) in adenovirus DNA replication. Ad pol is a member of the family… (more)

Subjects/Keywords: Geneeskunde; protein priming; DNA polymerase; replication; NFI; transcription factor; adenovirus; termination; initiation; pTP; origin

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Brenkman, A. B. (. B. (2002). Molecular architecture and function of adenovirus DNA polymerase. (Doctoral Dissertation). Universiteit Utrecht. Retrieved from http://dspace.library.uu.nl:8080/handle/1874/462

Chicago Manual of Style (16^th Edition):

Brenkman, A B (Arjan Bernard). “Molecular architecture and function of adenovirus DNA polymerase.” 2002. Doctoral Dissertation, Universiteit Utrecht. Accessed March 01, 2021. http://dspace.library.uu.nl:8080/handle/1874/462.

MLA Handbook (7^th Edition):

Brenkman, A B (Arjan Bernard). “Molecular architecture and function of adenovirus DNA polymerase.” 2002. Web. 01 Mar 2021.

Vancouver:

Brenkman AB(B. Molecular architecture and function of adenovirus DNA polymerase. [Internet] [Doctoral dissertation]. Universiteit Utrecht; 2002. [cited 2021 Mar 01]. Available from: http://dspace.library.uu.nl:8080/handle/1874/462.

Council of Science Editors:

Brenkman AB(B. Molecular architecture and function of adenovirus DNA polymerase. [Doctoral Dissertation]. Universiteit Utrecht; 2002. Available from: http://dspace.library.uu.nl:8080/handle/1874/462

Vanderbilt University

23. Guler, Gulfem Dilek. Human DNA helicase B in replication fork surveillance and replication stress recovery.

Degree: PhD, Biological Sciences, 2012, Vanderbilt University

URL: http://hdl.handle.net/1803/15350

► Correct and faithful genome duplication is crucial for preserving genomic integrity. Genome duplication is, therefore, highly regulated through a complex network of proteins that accomplish… (more)

▼ Correct and faithful genome duplication is crucial for preserving genomic integrity. Genome duplication is, therefore, highly regulated through a complex network of proteins that accomplish DNA replication in addition to DNA repair, as needed. Human DNA helicase B (HDHB) was previously proposed to function in DNA replication and DNA damage response. Work presented in this dissertation aimed to gain insight into the cellular pathway(s) HDHB participates in, particularly in response to replication stress. Contrary to previous studies where helicase-dead DHB inhibited DNA replication, we did not observe any cell cycle or DNA replication defect in HDHB silenced cells, suggesting that HDHB activity in DNA replication can be compensated by other proteins in the absence of HDHB. On the other hand, HDHB silencing disrupted efficient recovery from replication stress. HDHB silenced cells were capable of activating checkpoint signaling, suggesting a checkpoint-independent or downstream pathway for HDHB function. Consistent with a role in replication stress response, HDHB accumulated on chromatin upon UV, hydroxyurea and camptothecin exposure in a time- and dose-dependent manner. We found that genotoxin-induced HDHB accumulation on chromatin is RPA-dependent. Biophysical and biochemical characterization revealed a direct physical interaction between RPA70N basic cleft and a conserved acidic motif in HDHB helicase domain. The interaction interface between HDHB and RPA70N is strikingly similar to the previously reported interaction interfaces of RPA70N and p53, ATRIP, Rad9 or Mre11. Site-directed mutagenesis of the HDHB-RPA70N interaction interface demonstrated its contribution to HDHB recruitment to chromatin upon genotoxin exposure. These results altogether implicate HDHB in replication stress response. Advisors/Committee Members: Ellen Fanning (committee member), Daniel Kaplan (committee member), David Cortez (committee member), Walter J. Chazin (committee member), James G. Patton (Committee Chair).

Subjects/Keywords: Replication protein A; RPA; DNA helicase; DNA replication; DNA repair; replication stress; HelB; HDHB

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Guler, G. D. (2012). Human DNA helicase B in replication fork surveillance and replication stress recovery. (Doctoral Dissertation). Vanderbilt University. Retrieved from http://hdl.handle.net/1803/15350

Chicago Manual of Style (16^th Edition):

Guler, Gulfem Dilek. “Human DNA helicase B in replication fork surveillance and replication stress recovery.” 2012. Doctoral Dissertation, Vanderbilt University. Accessed March 01, 2021. http://hdl.handle.net/1803/15350.

MLA Handbook (7^th Edition):

Guler, Gulfem Dilek. “Human DNA helicase B in replication fork surveillance and replication stress recovery.” 2012. Web. 01 Mar 2021.

Vancouver:

Guler GD. Human DNA helicase B in replication fork surveillance and replication stress recovery. [Internet] [Doctoral dissertation]. Vanderbilt University; 2012. [cited 2021 Mar 01]. Available from: http://hdl.handle.net/1803/15350.

Council of Science Editors:

Guler GD. Human DNA helicase B in replication fork surveillance and replication stress recovery. [Doctoral Dissertation]. Vanderbilt University; 2012. Available from: http://hdl.handle.net/1803/15350

Queensland University of Technology

24. Chanson, Aurelie Heitiare. Recombinant protein production using a Tobacco yellow dwarf virus-based episomal expression vector : control of Rep activity.

Degree: 2009, Queensland University of Technology

URL: https://eprints.qut.edu.au/30290/

► Over the past decade, plants have been used as expression hosts for the production of pharmaceutically important and commercially valuable proteins. Plants offer many advantages… (more)

▼ Over the past decade, plants have been used as expression hosts for the production of pharmaceutically important and commercially valuable proteins. Plants offer many advantages over other expression systems such as lower production costs, rapid scale up of production, similar post-translational modification as animals and the low likelihood of contamination with animal pathogens, microbial toxins or oncogenic sequences. However, improving recombinant protein yield remains one of the greatest challenges to molecular farming. In-Plant Activation (InPAct) is a newly developed technology that offers activatable and high-level expression of heterologous proteins in plants. InPAct vectors contain the geminivirus cis elements essential for rolling circle replication (RCR) and are arranged such that the gene of interest is only expressed in the presence of the cognate viral replication-associated protein (Rep). The expression of Rep in planta may be controlled by a tissue-specific, developmentally regulated or chemically inducible promoter such that heterologous protein accumulation can be spatially and temporally controlled. One of the challenges for the successful exploitation of InPAct technology is the control of Rep expression as even very low levels of this protein can reduce transformation efficiency, cause abnormal phenotypes and premature activation of the InPAct vector in regenerated plants. Tight regulation over transgene expression is also essential if expressing cytotoxic products. Unfortunately, many tissue-specific and inducible promoters are unsuitable for controlling expression of Rep due to low basal activity in the absence of inducer or in tissues other than the target tissue. This PhD aimed to control Rep activity through the production of single chain variable fragments (scFvs) specific to the motif III of Tobacco yellow dwarf virus (TbYDV) Rep. Due to the important role played by the conserved motif III in the RCR, it was postulated that such scFvs can be used to neutralise the activity of the low amount of Rep expressed from a “leaky” inducible promoter, thus preventing activation of the TbYDV-based InPAct vector until intentional induction. Such scFvs could also offer the potential to confer partial or complete resistance to TbYDV, and possibly heterologous viruses as motif III is conserved between geminiviruses. Studies were first undertaken to determine the levels of TbYDV Rep and TbYDV replication-associated protein A (RepA) required for optimal transgene expression from a TbYDV-based InPAct vector. Transient assays in a non-regenerable Nicotiana tabacum (NT-1) cell line were undertaken using a TbYDV-based InPAct vector containing the uidA reporter gene (encoding GUS) in combination with TbYDV Rep and RepA under the control of promoters with high (CaMV 35S) or low (Banana bunchy top virus DNA-R, BT1) activity. The replication enhancer protein of Tomato leaf curl begomovirus (ToLCV), REn, was also used in some co-bombardment experiments to examine whether RepA could be substituted by a replication…

Subjects/Keywords: recombinant protein production; tobacco yellow dwarf virus; episomal expression vector; Rep activity

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Chanson, A. H. (2009). Recombinant protein production using a Tobacco yellow dwarf virus-based episomal expression vector : control of Rep activity. (Thesis). Queensland University of Technology. Retrieved from https://eprints.qut.edu.au/30290/

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Chanson, Aurelie Heitiare. “Recombinant protein production using a Tobacco yellow dwarf virus-based episomal expression vector : control of Rep activity.” 2009. Thesis, Queensland University of Technology. Accessed March 01, 2021. https://eprints.qut.edu.au/30290/.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Chanson, Aurelie Heitiare. “Recombinant protein production using a Tobacco yellow dwarf virus-based episomal expression vector : control of Rep activity.” 2009. Web. 01 Mar 2021.

Vancouver:

Chanson AH. Recombinant protein production using a Tobacco yellow dwarf virus-based episomal expression vector : control of Rep activity. [Internet] [Thesis]. Queensland University of Technology; 2009. [cited 2021 Mar 01]. Available from: https://eprints.qut.edu.au/30290/.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Chanson AH. Recombinant protein production using a Tobacco yellow dwarf virus-based episomal expression vector : control of Rep activity. [Thesis]. Queensland University of Technology; 2009. Available from: https://eprints.qut.edu.au/30290/

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Université Paris-Sud – Paris XI

25. Graindorge, Dany. Effets du rayonnement ultraviolet a sur la réplication de l’adn chez les eucaryotes supérieurs : Effects of ultraviolet radiation on the replication of DNA in higher eukaryotes.

Degree: Docteur es, Biologie cellulaire et moléculaire, 2012, Université Paris-Sud – Paris XI

URL: http://www.theses.fr/2012PA11T056

► Le rayonnement ultraviolet (UV) émis par le soleil et qui atteint la peau de chaque individu est composé majoritairement de photons UVA (λ de 315… (more)

▼ Le rayonnement ultraviolet (UV) émis par le soleil et qui atteint la peau de chaque individu est composé majoritairement de photons UVA (λ de 315 à 400 nm), le reste (5 à 10 %) étant composé d’UVB les plus longs (λ de 300 à 315 nm), car les radiations de longueur d’onde 300nm, c’est-à-dire les plus toxiques en terme de santé humaine, sont absorbées par la couche d’ozone stratosphérique. Contrairement aux UVB, les radiations UVA sont faiblement absorbées par l’ADN et de fait, génèrent peu de dimères cyclobutaniques de pyrimidines. Néanmoins, un des problèmes majeurs posés par une exposition aux UVA tient à ce que ce rayonnement excite certains composés endogènes photosensibles, inducteurs de la production d’espèces réactives de l’oxygène (ROS) qui peuvent alors endommager les composants cellulaires tels que les lipides,les acides nucléiques et les protéines. De ce fait, si les UVB restent le facteur étiologique majeur contribuant à la cancérogenèse cutanée photoinduite, un rôle des UVA, via la production de ROS, semble également émerger. Des précédents travaux obtenus au laboratoire ont montré que le rayonnement UVA ralentit la réplication de l’ADN, indépendamment de l’activation des points de contrôle du cycle cellulaire. Les auteurs ont émis l’hypothèse que les UVA, via l’oxydation des protéines, pouvaient altérer la machinerie de réplication. Mon travail de thèse a donc consisté à tenter de préciser le mécanisme qui gouverne ce retard de la réplication de l’ADN induit par les UVA dans les cellules de mammifères.Pour étudier au niveau moléculaire les effets des UVA sur la réplication, nous avons tout d’abord mis en place et utilisé au laboratoire la technique du peignage moléculaire (DNA combing) qui permet de mesurer divers paramètres de la réplication. Ainsi, nous montrons que le rayonnement UVA inhibe immédiatement et transitoirement les vitesses de fourches alors que l’inhibition sur l’initiation des origines est plus prolongée. Dans le cadre d’une collaboration, nous montrons également que les radiations UVA induisent une diminution modeste et transitoire du pool de dNTPs intracellulaires. La complémentation en ribonucléosides ne semble pas suffisante pour restaurer une vélocité normale de fourches immédiatement après UVA, ni la réplication dans sa totalité. En parallèle, nous observons l’oxydation réversible de la sous-unité R1 de la ribonucléotide réductase impliquée dans la biosynthèse des dNTPs. Bien que cette oxydation ne puisse expliquer la baisse transitoire du pool de nucléotides après UVA, nous ne pouvons pas exclure que d’autres formes d’oxydation de la RNR puissent affecter son activité.La présence d’azide de sodium (NaN3) au cours de l’irradiation UVA prévient le retard réplicatif, limite l’oxydation de la sous-unité R1 et la diminution du pool de dNTPs, ce qui démontre que ce retard de réplication est totalement dépendant des ROS, principalement de l’oxygène singulet généré pendant l’irradiation.L’ensemble de nos résultats indiquent que les UVA affectent le processus de réplication en modifiant non… Advisors/Committee Members: Sage, Evelyne (thesis director).

Subjects/Keywords: UVA; Stress oxydant; Réplication; Élongation; Initiation,; DNTPs; Ribonucléotide réductase; UVA; Oxidative stress; DNA replication; Elongation; Initiation; DNTPs; RRibonucleotide reductase.

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Graindorge, D. (2012). Effets du rayonnement ultraviolet a sur la réplication de l’adn chez les eucaryotes supérieurs : Effects of ultraviolet radiation on the replication of DNA in higher eukaryotes. (Doctoral Dissertation). Université Paris-Sud – Paris XI. Retrieved from http://www.theses.fr/2012PA11T056

Chicago Manual of Style (16^th Edition):

Graindorge, Dany. “Effets du rayonnement ultraviolet a sur la réplication de l’adn chez les eucaryotes supérieurs : Effects of ultraviolet radiation on the replication of DNA in higher eukaryotes.” 2012. Doctoral Dissertation, Université Paris-Sud – Paris XI. Accessed March 01, 2021. http://www.theses.fr/2012PA11T056.

MLA Handbook (7^th Edition):

Graindorge, Dany. “Effets du rayonnement ultraviolet a sur la réplication de l’adn chez les eucaryotes supérieurs : Effects of ultraviolet radiation on the replication of DNA in higher eukaryotes.” 2012. Web. 01 Mar 2021.

Vancouver:

Graindorge D. Effets du rayonnement ultraviolet a sur la réplication de l’adn chez les eucaryotes supérieurs : Effects of ultraviolet radiation on the replication of DNA in higher eukaryotes. [Internet] [Doctoral dissertation]. Université Paris-Sud – Paris XI; 2012. [cited 2021 Mar 01]. Available from: http://www.theses.fr/2012PA11T056.

Council of Science Editors:

Graindorge D. Effets du rayonnement ultraviolet a sur la réplication de l’adn chez les eucaryotes supérieurs : Effects of ultraviolet radiation on the replication of DNA in higher eukaryotes. [Doctoral Dissertation]. Université Paris-Sud – Paris XI; 2012. Available from: http://www.theses.fr/2012PA11T056

Hong Kong University of Science and Technology

26. Amin, Aftab. Studies of human and budding yeast DNA replication initiation proteins.

Degree: 2016, Hong Kong University of Science and Technology

URL: http://repository.ust.hk/ir/Record/1783.1-86342 ; https://doi.org/10.14711/thesis-b1610639 ; http://repository.ust.hk/ir/bitstream/1783.1-86342/1/th_redirect.html

► DNA replication is a stringently regulated cellular process. In proliferating normal cells, replication initiation proteins (RIPs) are sequentially loaded on to origins of replication during… (more)

▼ DNA replication is a stringently regulated cellular process. In proliferating normal cells, replication initiation proteins (RIPs) are sequentially loaded on to origins of replication during the M-to-G1 transition to form the pre-replication complex (pre-RC), in a process known as replication licensing. Critically, RIPs ensure chromosomal DNA is replicated only once per cell cycle, following origin activation. RIPs (Noc3p, Ipi3p, Cdt1p, Cdc6p, Mcm2-7p) are recruited by the origin recognition complex (ORC), which binds and marks replication origins throughout the cell cycle to form the pre-RC. The detailed mechanism(s) and regulation of the pre-RC and its architecture still remain unclear. In this study, 146 combinations of pairwise protein-protein interactions among 16 human pre-RC proteins were systematically and comprehensively examined for the first time. The yeast two-hybrid assay identified 72 positive interactions, of which 41 were previously unknown. hNOC3 and hIPI3 proteins had interactions with several RIPs, suggesting a role in DNA replication, similar to that found in the budding yeast homologs. The self-interactions of hORC2, -4, -5 and hNOC3 proteins were also of particular interest. Critically this study may provide a foundation for understanding the architecture(s) and function(s) of the human pre-RC. ORC self-interaction in the budding yeast was also examined to verify the Orc1p, -2p, -5p and -6p self-interactions. Subsequent experiments further supported the ORC dimerization mechanism. According to this model, ORC components self-interact to form dimer complexes (double-hexamers), at replication origins, prior to pre-RC formation. Following replication initiation, at origins in S phase, ORC double-hexamers dissociate into single-hexamers, which bind to the duplicated origins until late M phase, prior to replication-licensing. During the M-to-G1 transition, the double-hexamers, completing a semi-conservative, cell cycle dependent ORC 'dimerization cycle'. Depletion of non-chromatin bound ORC from the nucleus during the M-to-G1 transition abrogates ORC self-interactions, pre-RC formation and DNA replication. Three different yeast NOC3 temperature sensitive mutants were also examined in relation to DNA replication (ORC dimerization, pre-RC formation and maintenance, and cell cycle progression) and ribosome biogenesis in budding yeast to conclude that the functions of Noc3p in DNA replication and ribosome biogenesis are separable. Inactivation of Noc3p results in the failure of ORC dimerization, and MCM loading at the M-to-G1 transition and maintenance in G1 phase. Noc3p is the only non-ORC/MCM RIP that possesses self-interaction and potential dimerization. Noc3p and its self-interaction may be a prerequisite for ORC dimerization and eventual MCM double-hexamer loading. The 'ORC dimerization' model provides a symmetric platform to load the symmetric pre-RCs and guards against origin re-licensing within the same cell cycle by marking and protecting the nascent sister…

Subjects/Keywords: DNA replication ; DNA-protein interactions ; Protein-protein interactions ; Yeast

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APA (6^th Edition):

Amin, A. (2016). Studies of human and budding yeast DNA replication initiation proteins. (Thesis). Hong Kong University of Science and Technology. Retrieved from http://repository.ust.hk/ir/Record/1783.1-86342 ; https://doi.org/10.14711/thesis-b1610639 ; http://repository.ust.hk/ir/bitstream/1783.1-86342/1/th_redirect.html

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Amin, Aftab. “Studies of human and budding yeast DNA replication initiation proteins.” 2016. Thesis, Hong Kong University of Science and Technology. Accessed March 01, 2021. http://repository.ust.hk/ir/Record/1783.1-86342 ; https://doi.org/10.14711/thesis-b1610639 ; http://repository.ust.hk/ir/bitstream/1783.1-86342/1/th_redirect.html.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Amin, Aftab. “Studies of human and budding yeast DNA replication initiation proteins.” 2016. Web. 01 Mar 2021.

Vancouver:

Amin A. Studies of human and budding yeast DNA replication initiation proteins. [Internet] [Thesis]. Hong Kong University of Science and Technology; 2016. [cited 2021 Mar 01]. Available from: http://repository.ust.hk/ir/Record/1783.1-86342 ; https://doi.org/10.14711/thesis-b1610639 ; http://repository.ust.hk/ir/bitstream/1783.1-86342/1/th_redirect.html.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Amin A. Studies of human and budding yeast DNA replication initiation proteins. [Thesis]. Hong Kong University of Science and Technology; 2016. Available from: http://repository.ust.hk/ir/Record/1783.1-86342 ; https://doi.org/10.14711/thesis-b1610639 ; http://repository.ust.hk/ir/bitstream/1783.1-86342/1/th_redirect.html

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Vanderbilt University

27. Zamora Vargas, Paula Francisca. Functions of the nonstructural protein σNS in reovirus replication.

Degree: PhD, Microbiology and Immunology, 2018, Vanderbilt University

URL: http://hdl.handle.net/1803/12735

► Viral nonstructural proteins, which are not packaged into virions, are essential for the replication of most viruses. Reovirus, a nonenveloped, double-stranded RNA (dsRNA) virus, encodes… (more)

Subjects/Keywords: reovirus; dsRNA replication; RNA-binding protein; σNS

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Zamora Vargas, P. F. (2018). Functions of the nonstructural protein σNS in reovirus replication. (Doctoral Dissertation). Vanderbilt University. Retrieved from http://hdl.handle.net/1803/12735

Chicago Manual of Style (16^th Edition):

Zamora Vargas, Paula Francisca. “Functions of the nonstructural protein σNS in reovirus replication.” 2018. Doctoral Dissertation, Vanderbilt University. Accessed March 01, 2021. http://hdl.handle.net/1803/12735.

MLA Handbook (7^th Edition):

Zamora Vargas, Paula Francisca. “Functions of the nonstructural protein σNS in reovirus replication.” 2018. Web. 01 Mar 2021.

Vancouver:

Zamora Vargas PF. Functions of the nonstructural protein σNS in reovirus replication. [Internet] [Doctoral dissertation]. Vanderbilt University; 2018. [cited 2021 Mar 01]. Available from: http://hdl.handle.net/1803/12735.

Council of Science Editors:

Zamora Vargas PF. Functions of the nonstructural protein σNS in reovirus replication. [Doctoral Dissertation]. Vanderbilt University; 2018. Available from: http://hdl.handle.net/1803/12735

Vanderbilt University

28. Alers Rivera, Ileana. NMR analysis of the RPA dimer core RPA32D/14.

Degree: MS, Chemical and Physical Biology, 2011, Vanderbilt University

URL: http://hdl.handle.net/1803/13061

► CHEMICAL AND PHYSICAL BIOLOGY NMR STUDIES OF THE RPA DIMER CORE RPA32D/14 ILEANA ALERS RIVERA Thesis under the direction of Professor Walter J. Chazin Replication… (more)

Subjects/Keywords: telomeres; Replication Protein A; G-quadruplex

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Alers Rivera, I. (2011). NMR analysis of the RPA dimer core RPA32D/14. (Thesis). Vanderbilt University. Retrieved from http://hdl.handle.net/1803/13061

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Alers Rivera, Ileana. “NMR analysis of the RPA dimer core RPA32D/14.” 2011. Thesis, Vanderbilt University. Accessed March 01, 2021. http://hdl.handle.net/1803/13061.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Alers Rivera, Ileana. “NMR analysis of the RPA dimer core RPA32D/14.” 2011. Web. 01 Mar 2021.

Vancouver:

Alers Rivera I. NMR analysis of the RPA dimer core RPA32D/14. [Internet] [Thesis]. Vanderbilt University; 2011. [cited 2021 Mar 01]. Available from: http://hdl.handle.net/1803/13061.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Alers Rivera I. NMR analysis of the RPA dimer core RPA32D/14. [Thesis]. Vanderbilt University; 2011. Available from: http://hdl.handle.net/1803/13061

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Vanderbilt University

29. Riddle, Abigail Leigh. Characterizing the role of the Est1 protein in stimulating the recruitment of the Est3 protein to the yeast telomerase complex.

Degree: MS, Biological Sciences, 2012, Vanderbilt University

URL: http://hdl.handle.net/1803/12435

► Telomeres are protective protein/DNA complexes that cap the ends of linear chromosomes. The repetitive, TG-rich telomeric sequences are elongated by the telomerase ribonucleoprotein. In Saccharomyces… (more)

Subjects/Keywords: DNA replication; protein complex; assembly; cancer

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Riddle, A. L. (2012). Characterizing the role of the Est1 protein in stimulating the recruitment of the Est3 protein to the yeast telomerase complex. (Thesis). Vanderbilt University. Retrieved from http://hdl.handle.net/1803/12435

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Riddle, Abigail Leigh. “Characterizing the role of the Est1 protein in stimulating the recruitment of the Est3 protein to the yeast telomerase complex.” 2012. Thesis, Vanderbilt University. Accessed March 01, 2021. http://hdl.handle.net/1803/12435.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Riddle, Abigail Leigh. “Characterizing the role of the Est1 protein in stimulating the recruitment of the Est3 protein to the yeast telomerase complex.” 2012. Web. 01 Mar 2021.

Vancouver:

Riddle AL. Characterizing the role of the Est1 protein in stimulating the recruitment of the Est3 protein to the yeast telomerase complex. [Internet] [Thesis]. Vanderbilt University; 2012. [cited 2021 Mar 01]. Available from: http://hdl.handle.net/1803/12435.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Riddle AL. Characterizing the role of the Est1 protein in stimulating the recruitment of the Est3 protein to the yeast telomerase complex. [Thesis]. Vanderbilt University; 2012. Available from: http://hdl.handle.net/1803/12435

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Jawaharlal Nehru University

30. Mehra, Parul. Functional characterization of plasmodium falciparum replication initiation proteins; -.

Degree: Biology, 2007, Jawaharlal Nehru University

URL: http://shodhganga.inflibnet.ac.in/handle/10603/17572

None

References p.152-165

Advisors/Committee Members: Tyagi, Rakesh K, Dhar, Suman K.

Subjects/Keywords: Molecular Biology; Replication; plasmodium; falciparum; initiation

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Mehra, P. (2007). Functional characterization of plasmodium falciparum replication initiation proteins; -. (Thesis). Jawaharlal Nehru University. Retrieved from http://shodhganga.inflibnet.ac.in/handle/10603/17572

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Mehra, Parul. “Functional characterization of plasmodium falciparum replication initiation proteins; -.” 2007. Thesis, Jawaharlal Nehru University. Accessed March 01, 2021. http://shodhganga.inflibnet.ac.in/handle/10603/17572.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Mehra, Parul. “Functional characterization of plasmodium falciparum replication initiation proteins; -.” 2007. Web. 01 Mar 2021.

Vancouver:

Mehra P. Functional characterization of plasmodium falciparum replication initiation proteins; -. [Internet] [Thesis]. Jawaharlal Nehru University; 2007. [cited 2021 Mar 01]. Available from: http://shodhganga.inflibnet.ac.in/handle/10603/17572.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Mehra P. Functional characterization of plasmodium falciparum replication initiation proteins; -. [Thesis]. Jawaharlal Nehru University; 2007. Available from: http://shodhganga.inflibnet.ac.in/handle/10603/17572

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

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Open Access Theses and Dissertations