subject:(re suspension)

NSYSU

1. Wu, Chen-I. Using multi-frequency acoustic instruments to investigate the suspended sediment grain size and concentration characteristic in flume experiment and in the field.

Degree: Master, Marine Geology and chemistry, 2012, NSYSU

URL: http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0726112-142244

► In the past, the suspended sediment concentration (SSC) was mainly measured by the optical backscattering device (OBS) and water sample filtration. However, there has been… (more)

▼ In the past, the suspended sediment concentration (SSC) was mainly measured by the optical backscattering device (OBS) and water sample filtration. However, there has been a new development that user is based on the acoustics backscattering (ABS) to measure the SSC in the world. The acoustic instruments have some advantages that the optical ones do not have. For example, acoustic instruments are not effected by high turbidity, biofouling and high viscosity in the water. Acoustic instruments have high spatial and temporal resolutions. And they can immediately indicate the SSC changes than the water sample filtration method. Therefore, in this study we used the multi-frequency acoustics instrument (AQUAscat-1000) to investigate the relations of the suspended sediment size and concentration to the acoustic characteristics. The results are separated into two parts: (1) The calibration process in the flume. (2) The acoustic results in the field experiment. In the first part, we examined the range of preferred bin size with respect to the different frequencies as well as the condition in the flume, and determine that conclude the 20 mm is the best range for our case. In addition, the gain should be used when the maximum SSC is less than 100 mg/l and vice versa. Therefore, in the field case around the river mouth, the signal gain should be turned off due to high concentrations. According to the sensitivity of the backscatter intensity of different frequencies to the suspended particle sizes, multiple frequencies are needed to derive the SSC when the sediment size becomes smaller. The last point in this part is the comparison of the results between the acoustics and optical instruments. When the suspended particles in the water column are transparent, the optical measurement of the SSC is underestimated, but the acoustic method is not. The result of the field experiment in 2009 showed that the AQUAscat-1000 is a better instrument to quantify SSC than the optical instruments. The SSC increase caused by the bottom sediment re-suspension was due to the wave shear. In the 2011 experiment, although the acoustic results overestimated the SSC at some points but they still had higher relation and significance with water sample data than the optical measurements. The SSC increase caused by the bottom sediment re-suspension was due to the current shear. Advisors/Committee Members: Linus Y.S Chiu (chair), James T. Liu (committee member), Yu-Huai Wang (chair), Ruey-Chang Wei (chair).

Subjects/Keywords: flume; suspended sediment; multi-frequency acoustics; optical measurement; sediment re-suspension

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APA (6^th Edition):

Wu, C. (2012). Using multi-frequency acoustic instruments to investigate the suspended sediment grain size and concentration characteristic in flume experiment and in the field. (Thesis). NSYSU. Retrieved from http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0726112-142244

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Wu, Chen-I. “Using multi-frequency acoustic instruments to investigate the suspended sediment grain size and concentration characteristic in flume experiment and in the field.” 2012. Thesis, NSYSU. Accessed June 20, 2019. http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0726112-142244.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Wu, Chen-I. “Using multi-frequency acoustic instruments to investigate the suspended sediment grain size and concentration characteristic in flume experiment and in the field.” 2012. Web. 20 Jun 2019.

Vancouver:

Wu C. Using multi-frequency acoustic instruments to investigate the suspended sediment grain size and concentration characteristic in flume experiment and in the field. [Internet] [Thesis]. NSYSU; 2012. [cited 2019 Jun 20]. Available from: http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0726112-142244.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Wu C. Using multi-frequency acoustic instruments to investigate the suspended sediment grain size and concentration characteristic in flume experiment and in the field. [Thesis]. NSYSU; 2012. Available from: http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0726112-142244

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Virginia Commonwealth University

2. Sobotka, Molly. Legacy sediments in streams - effects on nutrient partitioning during simulated re-suspension events.

Degree: MS, Biology, 2011, Virginia Commonwealth University

URL: https://scholarscompass.vcu.edu/etd/2564

► Anthropogenic inputs of nutrients and sediment are a widespread problem in U.S. streams causing localized impairment and contributing to eutrophication of coastal habitats. Sediments and… (more)

Subjects/Keywords: Coastal Plains stream; nutrient; re-suspension; nitrogen; phosphorus; sediment; Biology; Life Sciences

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APA (6^th Edition):

Sobotka, M. (2011). Legacy sediments in streams - effects on nutrient partitioning during simulated re-suspension events. (Thesis). Virginia Commonwealth University. Retrieved from https://scholarscompass.vcu.edu/etd/2564

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Sobotka, Molly. “Legacy sediments in streams - effects on nutrient partitioning during simulated re-suspension events.” 2011. Thesis, Virginia Commonwealth University. Accessed June 20, 2019. https://scholarscompass.vcu.edu/etd/2564.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Sobotka, Molly. “Legacy sediments in streams - effects on nutrient partitioning during simulated re-suspension events.” 2011. Web. 20 Jun 2019.

Vancouver:

Sobotka M. Legacy sediments in streams - effects on nutrient partitioning during simulated re-suspension events. [Internet] [Thesis]. Virginia Commonwealth University; 2011. [cited 2019 Jun 20]. Available from: https://scholarscompass.vcu.edu/etd/2564.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Sobotka M. Legacy sediments in streams - effects on nutrient partitioning during simulated re-suspension events. [Thesis]. Virginia Commonwealth University; 2011. Available from: https://scholarscompass.vcu.edu/etd/2564

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

EPFL

3. Jenzer Althaus, Jolanda Maria Isabella. Sediment Evacuation from Reservoirs through Intakes by Jet Induced Flow.

Degree: 2011, EPFL

URL: http://infoscience.epfl.ch/record/154766

► Reservoir sedimentation is worldwide a significant long term problem and requires in view of the current mitigation measures an alternative and more sustainable solution. This… (more)

▼ Reservoir sedimentation is worldwide a significant long term problem and requires in view of the current mitigation measures an alternative and more sustainable solution. This challenge motivated the present study with the purpose to develop an alternative efficient method to release sediment out of a reservoir. The concept is based on the release of sediment through the headrace tunnel and turbines whereby a special focus was set on the fine sediment in the area in front of the power intakes. Specific jet arrangements should provide the energy and generate the optimum circulation needed to maintain the sediment in suspension and enhance its entrainment into the power intakes during turbining sequences. This new idea was experimentally tested in a rectangular laboratory tank with the following dimensions: 2 m wide, 1.5 m high and 4 m long. Two jet configurations were systematically investigated: a configuration of four jets arranged in a circle on a horizontal plane and a linear jet configuration located parallel to the front wall. The influence of the jet characteristics (nozzle diameter dj, jet velocity vj, jet discharge Qj, and jet angle θ) and the geometrical configuration parameters on the sediment release was investigated. As initial condition an almost homogeneous sediment concentration distribution was induced by air bubbles. This condition simulated a muddy layer like in front of the dam by the fading of a turbidity current. The water level during all the experiments was held constant by releasing the same discharge through the water intake as was introduced by the jets (experiments with jets) or through the back wall (experiments without jets), respectively. Turbidity measurements combined with flow velocity measurements gave information about the sediment release efficiency. The sediment release (evacuated sediment ratio, ESR) is defined as the evacuated sediment weight Pout divided by the sediment weight initially supplied Pin and represents the normalized temporal integral of the released sediment amount: ESR = Pout/Pin. Analogously, the settled sediment ratio is the settled sediment divided by the sediment weight initially supplied Pin. Experiments without jets as reference configuration showed an almost linear relation between the sediment release and the discharge within the tested range: the higher the discharge, the higher the evacuated sediment ratio. For a constant discharge the ultimate sediment release as well as the settled sediment ratio was easily estimated by a simple physical approach taking into account the settling velocity and the flow field generated by the discharge through the water intake and the back wall. For the tested discharge range the sediment release was between 0.09 and 0.37 for … Advisors/Committee Members: Schleiss, Anton, De Cesare, Giovanni.

Subjects/Keywords: reservoir sedimentation; suspended sediment; sediment release; resuspension; circular jet arrangement; UVP-measurements; turbidity measurements; axial mixer-like flow pattern; radial mixer-like flow pattern; jet mixing; numerical simulation; physical experiments; hydro-power plants; turbidity current; sédimentation des réservoirs; sédiments en suspension; évacuation des sédiments; re-suspension; arrangement circulaire de jets; mesures UVP; mesures de turbidité; champs de vitesse de type mélangeur axial; champs de vitesse de type mélangeur radial; mélanges de jets; simulation numérique; expériences physiques; aménagements hydrauliques; courants de turbidité

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Jenzer Althaus, J. M. I. (2011). Sediment Evacuation from Reservoirs through Intakes by Jet Induced Flow. (Thesis). EPFL. Retrieved from http://infoscience.epfl.ch/record/154766

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Jenzer Althaus, Jolanda Maria Isabella. “Sediment Evacuation from Reservoirs through Intakes by Jet Induced Flow.” 2011. Thesis, EPFL. Accessed June 20, 2019. http://infoscience.epfl.ch/record/154766.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Jenzer Althaus, Jolanda Maria Isabella. “Sediment Evacuation from Reservoirs through Intakes by Jet Induced Flow.” 2011. Web. 20 Jun 2019.

Vancouver:

Jenzer Althaus JMI. Sediment Evacuation from Reservoirs through Intakes by Jet Induced Flow. [Internet] [Thesis]. EPFL; 2011. [cited 2019 Jun 20]. Available from: http://infoscience.epfl.ch/record/154766.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Jenzer Althaus JMI. Sediment Evacuation from Reservoirs through Intakes by Jet Induced Flow. [Thesis]. EPFL; 2011. Available from: http://infoscience.epfl.ch/record/154766

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

4. Ossman, Mouheyddine. La culture matérielle de la Mésopotamie du Nord et de ses voisins, d’après l’étude de la céramique, de l’Uruk récent au Bronze ancien I/II : Material culture of Northern Mesopotamia and its Neighbours, according to ceramic study, from Late Uruk Period to Early Bronze Age I/II.

Degree: Docteur es, Langues, histoire et civilisations des mondes anciens, 2013, Université Lumière – Lyon II

URL: http://www.theses.fr/2013LYO20006

► Durant le IVe millénaire, la Mésopotamie et ses voisins ont été reliés via un vaste réseau commercial, établi par des Urukéens lors de leur expansion… (more)

▼ Durant le IVe millénaire, la Mésopotamie et ses voisins ont été reliés via un vaste réseau commercial, établi par des Urukéens lors de leur expansion en Iran et dans le Nord mésopotamien. À la fin du IVe et au début du IIIe, la Mésopotamie et ses voisins ont connu une phase de crise dont les causes nous sont toujours obscures en raison de l’absence des documents écrits remontant à cette phase. A cette phase, le réseau commercial urukéen et la colonisation urukéenne en Iran et en Mésopotamie du Nord, s’étant effondrés, les sites urukéens ont été abandonnés, ainsi qu’un grand nombre de sites indigènes qui ont été abandonnés. Le contact entre le Nord et le Sud mésopotamien fut suspendu. Pourtant, d’autres sites furent fondés que ce soit en Iran ou en Mésopotamie. Bien que la culture céramique mésopotamienne ne se soit modifiée profondément à cette phase cruciale, des traditions peintres réapparaissent soudainement alors que les Mésopotamiens les avaient abandonnées depuis 9 siècles (au Post-Obeid). Trois aires à céramique peinte apparaissent en Mésopotamie : en Irak du centre, de l’Est, du Nord (horizon de Ninive 5) et l’Iran a employé massivement la peinture. Cependant, le Sumer a conservé les traditions urukéennes qui ne connaissent pas de peinture. A noter que la peinture en Iran a été employée avant et durant l’expansion urukéenne. De plus, il semble avoir existé un corridor de contact reliant le West iranien aux trois zones à peinture en Mésopotamie. Mais comment peut-on expliquer ce phénomène de la réapparition de la peinture en Mésopotamie centrale et du Nord : s’agit-il des déplacements des groupes iraniens en Mésopotamie, ou des simples influences ? On ne peut pas traiter de la question de la fin du IVe/début du IIIe millénaire sans confronter, d’une part au problème concernant le destin des Urukéens. Ces derniers ont vécu plus de 4 siècles dans la périphérie durant l’expansion. Et d’autre part on est confronté au problème de la fondation des sites urbain aux touts début du 3e millénaire (Mari, Terqa, Kharab Sayyar et Chuera).Pour répondre à ces questions, nous avons choisi des sites-clés en Iran et en Mésopotamie. Leur céramique (et d’autre matériel) est étudié tout d’abord dans leur position stratigraphique, site par site, puis selon leur répartition dans l’espace. Au même temps, cette étude strato-céramique a été combinée avec une étude portée sur les changements dans l’occupation dans l’espace (abandon et fondation) et sur les transformations culturelles, surtout en Iran du Nord-Ouest et de l’Ouest où le phénomène de l’expansion transcaucasienne s’étend jusqu’au Kermanshah et le Nord du Luristan dans le Zagros central).Basant sur des comparaisons céramiques et sur d’autres aspects archéologiques, nous avons tenté de corréler entre la stratigraphie des sites étudiés. Nous avons évité de faire d’un site ou d’une région le « centre du monde ». Nous avons plutôt regardé chaque site et région à partir de ses voisins. A la fin de chaque partie ou chapitre, nous avons relié entre les régions étudiées, du point de… Advisors/Committee Members: Rouault, Olivier (thesis director).

Subjects/Keywords: Chalcolithique récent; Uruk; Uruk-Final; Transition; Mohammed Arab; Ninive 5; Djamdet Nasr; Proto-Élamite; Dynastique Archaïque; Bronze ancien; Early Jazire; Disparition de la peinture; Céramique; Réapparition de la peinture; Écarlate; Expansion; Retrait; Suspension; Reprise; Contact; Transcaucasien; Abandon; Fondation; Sumerianisation; Chute; Late Chalcolithic; Uruk; End-Uruk; Transition; Mohammed Arab; Nineveh 5; Jamdat Nasr; Proto-Elamite; Dynastic Archaic; Early Bronze Age; Early Jazire; Disappearance of painting; Ware; Ceramic; Reappearance of painting; Scarlet Ware; Suspension; Re-Establishment; Contact; Transcaucasian; Expansion; Uruk fall; Abandonment; Foundation; Sumerianisation

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Ossman, M. (2013). La culture matérielle de la Mésopotamie du Nord et de ses voisins, d’après l’étude de la céramique, de l’Uruk récent au Bronze ancien I/II : Material culture of Northern Mesopotamia and its Neighbours, according to ceramic study, from Late Uruk Period to Early Bronze Age I/II. (Doctoral Dissertation). Université Lumière – Lyon II. Retrieved from http://www.theses.fr/2013LYO20006

Chicago Manual of Style (16^th Edition):

Ossman, Mouheyddine. “La culture matérielle de la Mésopotamie du Nord et de ses voisins, d’après l’étude de la céramique, de l’Uruk récent au Bronze ancien I/II : Material culture of Northern Mesopotamia and its Neighbours, according to ceramic study, from Late Uruk Period to Early Bronze Age I/II.” 2013. Doctoral Dissertation, Université Lumière – Lyon II. Accessed June 20, 2019. http://www.theses.fr/2013LYO20006.

MLA Handbook (7^th Edition):

Ossman, Mouheyddine. “La culture matérielle de la Mésopotamie du Nord et de ses voisins, d’après l’étude de la céramique, de l’Uruk récent au Bronze ancien I/II : Material culture of Northern Mesopotamia and its Neighbours, according to ceramic study, from Late Uruk Period to Early Bronze Age I/II.” 2013. Web. 20 Jun 2019.

Vancouver:

Ossman M. La culture matérielle de la Mésopotamie du Nord et de ses voisins, d’après l’étude de la céramique, de l’Uruk récent au Bronze ancien I/II : Material culture of Northern Mesopotamia and its Neighbours, according to ceramic study, from Late Uruk Period to Early Bronze Age I/II. [Internet] [Doctoral dissertation]. Université Lumière – Lyon II; 2013. [cited 2019 Jun 20]. Available from: http://www.theses.fr/2013LYO20006.

Council of Science Editors:

Ossman M. La culture matérielle de la Mésopotamie du Nord et de ses voisins, d’après l’étude de la céramique, de l’Uruk récent au Bronze ancien I/II : Material culture of Northern Mesopotamia and its Neighbours, according to ceramic study, from Late Uruk Period to Early Bronze Age I/II. [Doctoral Dissertation]. Université Lumière – Lyon II; 2013. Available from: http://www.theses.fr/2013LYO20006

University of Western Australia

5. Kularatne, Kottabogoda Angidigedera Samantha Rangajeewa. Factors influencing sediment re-suspension and cross-shore suspended sediment flux in the frequency domain.

Degree: PhD, 2006, University of Western Australia

URL: http://repository.uwa.edu.au:80/R/?func=dbin-jump-full&object_id=8340&local_base=GEN01-INS01

►

[Truncated abstract] With rapidly increasing population densities along coastlines and rising global sea levels, coastal protection has become a major concern for coastal communities. Predicting… (more)

▼

[Truncated abstract] With rapidly increasing population densities along coastlines and rising global sea levels, coastal protection has become a major concern for coastal communities. Predicting sediment transport in nearshore regions, however, is one of the most challenging tasks faced by coastal researchers in designing coastal structures or beach nourishment schemes. Although nearshore sediment transport mainly occurs in the longshore direction, cross-shore sediment transport is crucial in determining the shoreline evolution and beach morphology . . . This study investigated the factors influencing sediment re-suspension and cross-shore suspended sediment flux in the frequency domain through a series of field measurements conducted at several different locations and a numerical model. Only oscillatory flow components were examined and the mean flow components were not considered. Although many different factors such as cross-shore location with respect to breaker line, significant wave height to water depth ratio (Hs/h), normalised horizontal velocity skewness (<u³>/‹u²›³/²), median grain size (d50), breaker type, and wave groupiness appeared to influence the magnitude of cross-shore suspended sediment flux, bed ripples was identified as the major contributing factor in changing the direction of suspended sediment flux due to incident swell waves. Moreover, the direction changed significantly with ripple type. High frequency measurements, obtained to examine the influence of turbulent kinetic energy (TKE) on higher sediment suspension events observed under wave groups indicated that higher TKE was generated at the seabed by approaching wave groups, which in turn resulted in higher suspension events.

[Truncated abstract] With rapidly increasing population densities along coastlines and rising global sea levels, coastal protection has become a major concern for coastal communities. Predicting sediment transport in nearshore regions, however, is one of the most challenging tasks faced by coastal researchers in designing coastal structures or beach nourishment schemes. Although nearshore sediment transport mainly occurs in the longshore direction, cross-shore sediment transport is crucial in determining the shoreline evolution and beach morphology . . . This study investigated the factors influencing sediment re-suspension and cross-shore suspended sediment flux in the frequency domain through a series of field measurements conducted at several different locations and a numerical model. Only oscillatory flow components were examined and the mean flow components were not considered. Although many different factors such as cross-shore location with respect to breaker line, significant wave height to water depth ratio (Hs/h), normalised horizontal velocity skewness (<u³>/‹u²›³/²), median grain size (d50), breaker type, and wave groupiness appeared to influence the magnitude of cross-shore suspended sediment flux, bed ripples was identified as the major…

Subjects/Keywords: Coastal engineering; Coast changes; Beach erosion; Sediment transport; Suspended sediments; Sediment re-suspension; Bed ripples; Cross-shore sediment flux; Turbulant kinetic energy

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APA (6^th Edition):

Kularatne, K. A. S. R. (2006). Factors influencing sediment re-suspension and cross-shore suspended sediment flux in the frequency domain. (Doctoral Dissertation). University of Western Australia. Retrieved from http://repository.uwa.edu.au:80/R/?func=dbin-jump-full&object_id=8340&local_base=GEN01-INS01

Chicago Manual of Style (16^th Edition):

Kularatne, Kottabogoda Angidigedera Samantha Rangajeewa. “Factors influencing sediment re-suspension and cross-shore suspended sediment flux in the frequency domain.” 2006. Doctoral Dissertation, University of Western Australia. Accessed June 20, 2019. http://repository.uwa.edu.au:80/R/?func=dbin-jump-full&object_id=8340&local_base=GEN01-INS01.

MLA Handbook (7^th Edition):

Kularatne, Kottabogoda Angidigedera Samantha Rangajeewa. “Factors influencing sediment re-suspension and cross-shore suspended sediment flux in the frequency domain.” 2006. Web. 20 Jun 2019.

Vancouver:

Kularatne KASR. Factors influencing sediment re-suspension and cross-shore suspended sediment flux in the frequency domain. [Internet] [Doctoral dissertation]. University of Western Australia; 2006. [cited 2019 Jun 20]. Available from: http://repository.uwa.edu.au:80/R/?func=dbin-jump-full&object_id=8340&local_base=GEN01-INS01.

Council of Science Editors:

Kularatne KASR. Factors influencing sediment re-suspension and cross-shore suspended sediment flux in the frequency domain. [Doctoral Dissertation]. University of Western Australia; 2006. Available from: http://repository.uwa.edu.au:80/R/?func=dbin-jump-full&object_id=8340&local_base=GEN01-INS01

University of Florida

6. Cho, Hwan Chul. Physical and CFD Models of PM Separtion and Scour in Hydrodynamic Unit Operation.

Degree: PhD, Environmental Engineering Sciences, 2012, University of Florida

URL: http://ufdc.ufl.edu/UFE0044037

Subjects/Keywords: Charge flow devices; Diameters; Flow velocity; Hydrodynamics; Modeling; Particulate materials; Rain; Separators; Storms; Velocity; cfd; pm; re-suspension; runoff; scour; stormwater; uop

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APA (6^th Edition):

Cho, H. C. (2012). Physical and CFD Models of PM Separtion and Scour in Hydrodynamic Unit Operation. (Doctoral Dissertation). University of Florida. Retrieved from http://ufdc.ufl.edu/UFE0044037

Chicago Manual of Style (16^th Edition):

Cho, Hwan Chul. “Physical and CFD Models of PM Separtion and Scour in Hydrodynamic Unit Operation.” 2012. Doctoral Dissertation, University of Florida. Accessed June 20, 2019. http://ufdc.ufl.edu/UFE0044037.

MLA Handbook (7^th Edition):

Cho, Hwan Chul. “Physical and CFD Models of PM Separtion and Scour in Hydrodynamic Unit Operation.” 2012. Web. 20 Jun 2019.

Vancouver:

Cho HC. Physical and CFD Models of PM Separtion and Scour in Hydrodynamic Unit Operation. [Internet] [Doctoral dissertation]. University of Florida; 2012. [cited 2019 Jun 20]. Available from: http://ufdc.ufl.edu/UFE0044037.

Council of Science Editors:

Cho HC. Physical and CFD Models of PM Separtion and Scour in Hydrodynamic Unit Operation. [Doctoral Dissertation]. University of Florida; 2012. Available from: http://ufdc.ufl.edu/UFE0044037

EPFL

7. Seger, Urban. Electrical cell manipulation in microfluidic systems.

Degree: 2006, EPFL

URL: http://infoscience.epfl.ch/record/86087

► This dissertation reports on the development of devices and concepts for electrical and microfluidic cell manipulation. In the present context, the term cell manipulation stands… (more)

▼ This dissertation reports on the development of devices and concepts for electrical and microfluidic cell manipulation. In the present context, the term cell manipulation stands for both cell handling and cell modification. The combination of microfluidic channels with micropatterned electrodes allows for the definition of highly localised chemical and electrical environments with spatial resolution comparable to the size of a cell. The devices fabricated in the frame of this thesis employ dielectrophoretic particle handling schemes such as deflection and trapping in pressure-controlled laminar flows to bring cells to – or immobilise them at – locations where cell altering electric fields or chemicals are present. The two concepts of dielectrophoretic cell dipping and cell immersion are introduced and experimentally shown for erythrocytes dipped into Rhodamine in flow, and for individually immobilised Jurkat cells immersed by Trypan Blue. Also, in-situ membrane breakdown in high intensity AC electric fields is optically assessed by efflux of haemoglobin (haemolysis) and by influx of nucleic stains or fluorescence-enhancing ions. The most advanced experiments are on-chip medium exchange followed immediately by electropermeablisation or electrodeformation. The majority of assays presented in this thesis are carried out in microfabricated glass-polymer-glass chips featuring top-bottom electrodes. The devices are fluidically controlled by external gas pressure bridging circuits. Experimental evidence of the unmatched precision of pressure bridging is given in the case of micrometric xy positioning of cells at the intersection of two perpendicular microfluidic channels. Further shown in this document are two methods of optical in-situ temperature measurements, important for bioinstrument characterisation. The two concepts of thermoquenching of a fluorescent dye and the original thermoprecipitation of "smart polymers" are used. The last part of this work deals with the innovative, conceptual engineering tool Liquid Electrode. The general concept and its advantages over solid-state electrodes are given, followed by numerical particle tracking in the case of the novel lateral nDEP particle deflection. The chapter on liquid electrodes concludes with preliminary experimental results of buffer swapping of cells in flow and of AC electropermeabilisation of erythrocytes at frequencies far below the cut-off frequency of corresponding solid-state microelectrodes. Advisors/Committee Members: Renaud, Philippe.

Subjects/Keywords: lab-on-a-chip; pressure-balanced flow; cell positioning; dielectrophoresis; single-cell trapping; cell dipping; re-suspension; electropermeabilisation; cell lysis; dipped electrodeformation; fluorescence; Joule heating; thermoprecipitation; liquid electrodes; lab-on-a-chip; flux équilibré par pression; positionnement de cellules; diélectrophorèse; piégeage de cellules; cell dipping; resuspension; électroperméabilisation; lyse cellulaire; électrodéformation; fluorescence; effet Joule; thermoprécipitation; électrodes liquides

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Seger, U. (2006). Electrical cell manipulation in microfluidic systems. (Thesis). EPFL. Retrieved from http://infoscience.epfl.ch/record/86087

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Seger, Urban. “Electrical cell manipulation in microfluidic systems.” 2006. Thesis, EPFL. Accessed June 20, 2019. http://infoscience.epfl.ch/record/86087.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Seger, Urban. “Electrical cell manipulation in microfluidic systems.” 2006. Web. 20 Jun 2019.

Vancouver:

Seger U. Electrical cell manipulation in microfluidic systems. [Internet] [Thesis]. EPFL; 2006. [cited 2019 Jun 20]. Available from: http://infoscience.epfl.ch/record/86087.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Seger U. Electrical cell manipulation in microfluidic systems. [Thesis]. EPFL; 2006. Available from: http://infoscience.epfl.ch/record/86087

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Open Access Theses and Dissertations