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The Ohio State University

1. Baldauff, Rachel Michelle. Interaction of Recombinant Paraoxonase-1 with Reconstituted High-Density Lipoproteins.

Degree: MS, Chemistry, 2011, The Ohio State University

Overwhelming evidence suggests that low levels of high-density lipoproteins (HDL) increase one’s odds of developing coronary artery disease (CAD). This phenomenon could be due in part to the hydrolase activity of the enzyme human serum paraoxonase-1 (huPON1) that is found bound to the HDL in the circulatory system. Many studies have indicated lowered atherogenic effects due to raised huPON1/HDL levels. In order to fully understand these correlations, more insight into how the interaction of huPON1 and HDL may be delivering its beneficial properties is needed. In the current study, the interactions between reconstituted HDL (rHDL) particles and fluorophore-labeled variants of PON1 (G3C9-ybbR and five other variants) were probed via fluorescence anisotropy, effects on arylesterase and paraoxonase activities, and changes in arylesterase activity upon binding to rHDL. While the mutations to the hydrophobic signal sequence (L12A and L12K) did not appear to have significant effects on the arylesterase or paraoxonase activities, the mutations on the active site lid (W194A and W194K) caused increases in both activities. While the fluorescence anisotropy measurements were unable to reveal anything about the binding affinity, the arylesterase stimulation due to rHDL binding did. Advisors/Committee Members: Gustafson, Terry (Advisor), Magliery, Thomas (Advisor).

Subjects/Keywords: Biochemistry; rePON1; rHDL; G3C9

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APA (6th Edition):

Baldauff, R. M. (2011). Interaction of Recombinant Paraoxonase-1 with Reconstituted High-Density Lipoproteins. (Masters Thesis). The Ohio State University. Retrieved from http://rave.ohiolink.edu/etdc/view?acc_num=osu1321986125

Chicago Manual of Style (16th Edition):

Baldauff, Rachel Michelle. “Interaction of Recombinant Paraoxonase-1 with Reconstituted High-Density Lipoproteins.” 2011. Masters Thesis, The Ohio State University. Accessed August 12, 2020. http://rave.ohiolink.edu/etdc/view?acc_num=osu1321986125.

MLA Handbook (7th Edition):

Baldauff, Rachel Michelle. “Interaction of Recombinant Paraoxonase-1 with Reconstituted High-Density Lipoproteins.” 2011. Web. 12 Aug 2020.

Vancouver:

Baldauff RM. Interaction of Recombinant Paraoxonase-1 with Reconstituted High-Density Lipoproteins. [Internet] [Masters thesis]. The Ohio State University; 2011. [cited 2020 Aug 12]. Available from: http://rave.ohiolink.edu/etdc/view?acc_num=osu1321986125.

Council of Science Editors:

Baldauff RM. Interaction of Recombinant Paraoxonase-1 with Reconstituted High-Density Lipoproteins. [Masters Thesis]. The Ohio State University; 2011. Available from: http://rave.ohiolink.edu/etdc/view?acc_num=osu1321986125

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