subject:(quorum quenching)

1. Conrado, Isabel Monteiro. Bactérias e as suas redes sociais.

Degree: 2013, Universidade Fernando Pessoa

URL: http://www.rcaap.pt/detail.jsp?id=oai:bdigital.ufp.pt:10284/4168

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Projeto de Pós-Graduação/Dissertação apresentado à Universidade Fernando Pessoa como parte dos requisitos para obtenção do grau de Mestre em Ciências Farmacêuticas

As bactérias são microorganismos… (more)

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Projeto de Pós-Graduação/Dissertação apresentado à Universidade Fernando Pessoa como parte dos requisitos para obtenção do grau de Mestre em Ciências Farmacêuticas

As bactérias são microorganismos primitivos unicelulares que habitam em nichos ecológicos formando comunidades multiespécie e sintetizam moléculas sinalizadoras que lhes permitem comunicar entre si por um mecanismo designado de quorum sensing. O primeiro modelo experimental de quorum sensing a ser estudado foi o da bactéria de Gram-negativo bioluminescente Vibrio fischeri que sintetiza como moléculas de sinalização as N-acil-homoserina lactonas. As bactérias de Gram-negativo comunicam igualmente através de outras moléculas de sinalização nomeadamente, 2-alquil-4-quinolonas (AQs), cadeias longas de ácidos gordos e ésteres metílicos de ácidos gordos. As bactérias de Gram-positivo sintetizam auto-indutores peptídicos. Nesta revisão bibliográfica estão descritos alguns mecanismos de quorum sensing, designadamente, o sistema LuxI/LuxR pela bactéria Víbrio fischeri, sistema de quorum sensing peptídico em Staphylococcus aureus, circuitos de quorum sensing paralelos por Vibrio harveyi, circuitos de quorum sensing competitivos por Bacillus subtilis e circuitos de quorum sensing organizados em série por Pseudomonas aeruginosa. Serão igualmente abordadas novas terapêuticas antibacterianas que incluem a utilização de moléculas anti-quorum sensing. Bacteria are primitive unicellular microorganisms that inhabit ecological niches composed by multispecific communities and synthesize signal molecules that allow them to communicate between each other by quorum sensing. The first studied model of quorum sensing was of the bioluminescent Gram-negative bacteria Vibrio fischeri, which synthesize N-Acyl homoserine lactone signal molecules. Gram-negative bacteria also communicate using other signal molecules, namely 2-alkyl-4-quinolones (AQs), long-chain fatty acids and fatty acid ethyl ester. Gram-positive bacteria, however, synthesize peptide autoinducers. In this review several quorum sensing mechanisms are described, namely the LuxI/LuxR system by the bacteria Vibrio fischeri, peptide quorum sensing in Staphylococcus aureus, parallel quorum sensing circuits by Vibrio harveyi, competitive quorum sensing circuits by Bacillus subtilis and serial quorum sensing circuits by Pseudomonas aeruginosa. Novel antibacterial therapeutics that include the use of anti-quorum sensing molecules will also be approached.

Advisors/Committee Members: Castro, Anabela.

Subjects/Keywords: Bactérias; Comunicação entre as bactérias; Quorum sensing; Quorum quenching; Bacteria; Cross-talk in bacteria; Quorum sensing; Quorum quenching

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Conrado, I. M. (2013). Bactérias e as suas redes sociais. (Thesis). Universidade Fernando Pessoa. Retrieved from http://www.rcaap.pt/detail.jsp?id=oai:bdigital.ufp.pt:10284/4168

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Conrado, Isabel Monteiro. “Bactérias e as suas redes sociais.” 2013. Thesis, Universidade Fernando Pessoa. Accessed January 27, 2021. http://www.rcaap.pt/detail.jsp?id=oai:bdigital.ufp.pt:10284/4168.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Conrado, Isabel Monteiro. “Bactérias e as suas redes sociais.” 2013. Web. 27 Jan 2021.

Vancouver:

Conrado IM. Bactérias e as suas redes sociais. [Internet] [Thesis]. Universidade Fernando Pessoa; 2013. [cited 2021 Jan 27]. Available from: http://www.rcaap.pt/detail.jsp?id=oai:bdigital.ufp.pt:10284/4168.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Conrado IM. Bactérias e as suas redes sociais. [Thesis]. Universidade Fernando Pessoa; 2013. Available from: http://www.rcaap.pt/detail.jsp?id=oai:bdigital.ufp.pt:10284/4168

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Université Paris-Sud – Paris XI

2. Tannières, Mélanie. Interruption de la communication bactérienne dans la rhizosphère par la dégradation enzymatique des signaux quorum sensing : Disruption of bacterial communication in rhizosphere by enzymatic degradation of quorum sensing signals.

Degree: Docteur es, Biologie, 2012, Université Paris-Sud – Paris XI

URL: http://www.theses.fr/2012PA112050

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L’identification, chez divers organismes, d’enzymes de dégradation des N-acyl homosérineslactones (NAHLs) impliquées dans la signalisation QS pose la question de leurs rôles dans lesinteractions bactéries-eucaryotes.… (more)

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L’identification, chez divers organismes, d’enzymes de dégradation des N-acyl homosérineslactones (NAHLs) impliquées dans la signalisation QS pose la question de leurs rôles dans lesinteractions bactéries-eucaryotes. Dans une première partie, une synthèse bibliographique analyse lesconnaissances acquises sur ces enzymes dégradant les NAHLs. Dans une seconde partie, la croissancedes bactéries dégradant les signaux NAHLs a été stimulée par l’application de g-caprolactone (GCL)dans la rhizosphère de plants de pommes de terre à des fins de phytoprotection. L’effet de cetraitement sur la diversité des communautés bactériennes rhizosphériques a été évalué en combinantdifférentes approches d’écologie microbienne moléculaire comme la DGGE, le pyroséquençaged’amplicons rrs, et la métagénomique fonctionnelle. Cette dernière approche appliquée à une banquede 30 000 clones environ a conduit à l’identification d’un gène qsdB codant la dégradation des signauxNAHL. Ce travail révèle ainsi l’existence d’une nouvelle classe d’enzymes de dégradation des NAHLsappartenant à la famille des enzymes possédant une signature amidase (AS) dont des membres sontpar ailleurs impliqués dans la dégradation de composés xénobiotiques. Dans une troisième partie, unsystème expérimental a été développé afin de mesurer le transfert conjugatif du plasmide de virulenceTi (tumor inducing) chez des dérivés du pathogène Agrobacterium tumefaciens, appelés «tricheurs»,incapables de produire des signaux NAHLs mais utilisateurs de ceux produits par les autres bactéries.Ce modèle a permis de montrer l’effet modérateur de lactonases dégradant les NAHLs exprimées chezdes agrobactéries produisant les NAHLs, chez des bactéries réceptrices du plasmide Ti, ou des planteshôtes des agrobactéries sur le transfert conjugatif initié par les tricheurs. L’ensemble de ce travailrévèle à la fois une nouvelle famille d’enzymes impliquées dans la dégradation des NAHLs, ainsiqu’un nouveau rôle de ces enzymes dans la modulation des flux de gènes entre bactériesphytopathogènes en interaction avec une plante hôte.

Identification of bacterial and eukaryotic enzymes that degrade N-acyl homoserine lactones(NAHLs) involved in QS signaling raises the question of their roles in bacteria-eucaryotesinteractions. In a first part of this study, a bibliographic report analyzes the current data on thoseNAHL-degrading enzymes. In a second part, the growth of NAHL-degrading bacteria was stimulatedby g-caprolactone (GCL) amendment in potato rhizosphere to protect this plant against the soft-rotpathogen Pectobacterium. The effect of the GCL treatment on rhizospheric bacterial communities wasevaluated by a combination of different molecular microbial ecology techniques such as DGGE,pyrosequencing and functional metagenomic. This last approach was applied to generate ametagenomic library of ca. 30,000 clones and lead to the identification of the qsdB gene that encodesNAHL degradation, This work revealed the occurrence of a novel class of NAHL-degrading enzymesthat belong to the amidase signature…

Advisors/Committee Members: Faure, Denis (thesis director).

Subjects/Keywords: Quorum sensing; Quorum quenching; Métagénomique; AS family; Tricheur; Agrobacterium; Quorum sensing; Quorum quenching; Metagenomics; AS family; Cheaters; Agrobacterium

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Tannières, M. (2012). Interruption de la communication bactérienne dans la rhizosphère par la dégradation enzymatique des signaux quorum sensing : Disruption of bacterial communication in rhizosphere by enzymatic degradation of quorum sensing signals. (Doctoral Dissertation). Université Paris-Sud – Paris XI. Retrieved from http://www.theses.fr/2012PA112050

Chicago Manual of Style (16^th Edition):

Tannières, Mélanie. “Interruption de la communication bactérienne dans la rhizosphère par la dégradation enzymatique des signaux quorum sensing : Disruption of bacterial communication in rhizosphere by enzymatic degradation of quorum sensing signals.” 2012. Doctoral Dissertation, Université Paris-Sud – Paris XI. Accessed January 27, 2021. http://www.theses.fr/2012PA112050.

MLA Handbook (7^th Edition):

Tannières, Mélanie. “Interruption de la communication bactérienne dans la rhizosphère par la dégradation enzymatique des signaux quorum sensing : Disruption of bacterial communication in rhizosphere by enzymatic degradation of quorum sensing signals.” 2012. Web. 27 Jan 2021.

Vancouver:

Tannières M. Interruption de la communication bactérienne dans la rhizosphère par la dégradation enzymatique des signaux quorum sensing : Disruption of bacterial communication in rhizosphere by enzymatic degradation of quorum sensing signals. [Internet] [Doctoral dissertation]. Université Paris-Sud – Paris XI; 2012. [cited 2021 Jan 27]. Available from: http://www.theses.fr/2012PA112050.

Council of Science Editors:

Tannières M. Interruption de la communication bactérienne dans la rhizosphère par la dégradation enzymatique des signaux quorum sensing : Disruption of bacterial communication in rhizosphere by enzymatic degradation of quorum sensing signals. [Doctoral Dissertation]. Université Paris-Sud – Paris XI; 2012. Available from: http://www.theses.fr/2012PA112050

3. Bzdrenga, Janek. De la promiscuité des enzymes : cas des PLLs et de leur implication dans l'anti virulence bactérienne et la décontamination des organophosphorés : Enzymatic promiscuity : the case of PLLs and their implication in bacterial anti virulence and organophosphate decontamination.

Degree: Docteur es, Pathologie humaine. Maladies infectieuses, 2016, Aix Marseille Université

URL: http://www.theses.fr/2016AIXM5018

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Les PLLs sont une famille d’enzyme dont l’activité catalytique est double. L’activité lactonase permet entre autre de détruire les AHLs, molécules médiatrices de la communication… (more)

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Les PLLs sont une famille d’enzyme dont l’activité catalytique est double. L’activité lactonase permet entre autre de détruire les AHLs, molécules médiatrices de la communication chez les bactéries Gram négatives, empêchant ainsi la synchronisation à l’échelle de la population de la sécrétion de facteurs de virulence ou la formation de biofilm. Elles sont non seulement capables d’hydrolyser les molécules possédant un noyau lactone, mais aussi les phosphotriésters par promiscuité de substrat. L’activité phosphotriestérase permet de dégrader les composés organophosphorés (OPs) hautement toxiques, que ce soit les insecticides (Paraoxon) ou les agents neurotoxiques de guerre (Sarin, VX). Les travaux effectués ont consisté à évaluer l’efficacité de la PLL SsoPox, provenant de l’archée extrêmophile Sulfolobus solfataricus, pour empêcher la mise en place de la virulence et du biofilm chez Pseudomonas aeruginosa PAO1. Cette évaluation a été effectuée in vitro et in vivo via un modèle d’infection pulmonaire chez le rat. D’autre part, une étude in vitro a été réalisée sur 73 souches cliniques de P. aeruginosa isolés sur des patients atteints de pied diabétique, en évaluant la quantité de pyocyanine et l’activité protéolytique. Enfin, une phase prospective pour identifier de nouvelles PLLs a permis la caractérisation enzymatique et structurale de 2 nouvelles enzymes, SacPox et VmoLac, contribuant ainsi à affiner la connaissance sur cette famille et leur potentiel d’amélioration par ingénierie protéique. Au final, les PLLs offrent un intérêt biotechnologique majeur et peuvent mener à une valorisation concrète pour la santé humaine mais également pour la bioremédiation des OPs.

Phosphotriesterase-Like Lactonases (PLLs) are a family of enzyme displaying a dual catalytic activity. Lactonase activity allows for, among others, the destruction of Acyl Homoserine Lactones (AHLs). These molecules mediate the communication in Gram negative bacteria, allowing them to synchronize group behavior like virulence factor secretion or biofilm formation. Beside their ability to hydrolyze molecules with a lactones moiety, PLL also show substrate promiscuity for hydrolyzing highly toxic organophosphate compounds (OPs), such as pesticides (i.e. Paraoxon) or Chemical Warfare Nerve Agents (CWNAs) (e.g. Sarin & VX). The work described here consisted in evaluating the efficacy of the PLL SsoPox, originating from the extremophile Archaea Sulfolobus solfataricus, to prevent virulence and biofilm formation on the model strain Pseudomonas aeruginosa PAO1. This evaluation was performed both in vitro and in vivo, by using a rat pulmonary infection model. A study has been performed on a strain collection of 73 clinical strains of P. aeruginosa isolated from diabetic foot, to assess the enzyme effects on pyocyanin secretion and protease activity. Finally, a prospective phase to identify new PLLs allowed for the enzymatic and structural characterization of two new enzymes, SacPox and VmoLac, thus contributing to refine the knowledge about this enzyme family…

Advisors/Committee Members: Chabrière, Eric (thesis director).

Subjects/Keywords: Quorum sensing; Quorum quenching; Virulence; Lactonase; Biofilm; Enzyme; Quorum sensing; Quorum quenching; Virulence; Lactonase; Biofilm; Enzyme

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Bzdrenga, J. (2016). De la promiscuité des enzymes : cas des PLLs et de leur implication dans l'anti virulence bactérienne et la décontamination des organophosphorés : Enzymatic promiscuity : the case of PLLs and their implication in bacterial anti virulence and organophosphate decontamination. (Doctoral Dissertation). Aix Marseille Université. Retrieved from http://www.theses.fr/2016AIXM5018

Chicago Manual of Style (16^th Edition):

Bzdrenga, Janek. “De la promiscuité des enzymes : cas des PLLs et de leur implication dans l'anti virulence bactérienne et la décontamination des organophosphorés : Enzymatic promiscuity : the case of PLLs and their implication in bacterial anti virulence and organophosphate decontamination.” 2016. Doctoral Dissertation, Aix Marseille Université. Accessed January 27, 2021. http://www.theses.fr/2016AIXM5018.

MLA Handbook (7^th Edition):

Bzdrenga, Janek. “De la promiscuité des enzymes : cas des PLLs et de leur implication dans l'anti virulence bactérienne et la décontamination des organophosphorés : Enzymatic promiscuity : the case of PLLs and their implication in bacterial anti virulence and organophosphate decontamination.” 2016. Web. 27 Jan 2021.

Vancouver:

Bzdrenga J. De la promiscuité des enzymes : cas des PLLs et de leur implication dans l'anti virulence bactérienne et la décontamination des organophosphorés : Enzymatic promiscuity : the case of PLLs and their implication in bacterial anti virulence and organophosphate decontamination. [Internet] [Doctoral dissertation]. Aix Marseille Université 2016. [cited 2021 Jan 27]. Available from: http://www.theses.fr/2016AIXM5018.

Council of Science Editors:

Bzdrenga J. De la promiscuité des enzymes : cas des PLLs et de leur implication dans l'anti virulence bactérienne et la décontamination des organophosphorés : Enzymatic promiscuity : the case of PLLs and their implication in bacterial anti virulence and organophosphate decontamination. [Doctoral Dissertation]. Aix Marseille Université 2016. Available from: http://www.theses.fr/2016AIXM5018

Virginia Tech

4. Leaman, Eric Joshua. An Experimentally-validated Agent-based Model to Study the Emergent Behavior of Bacterial Communities.

Degree: MS, Mechanical Engineering, 2016, Virginia Tech

URL: http://hdl.handle.net/10919/78072

► Swimming bacteria are ubiquitous in aqueous environments ranging from oceans to fluidic environments within a living host. Furthermore, engineered bacteria are being increasingly utilized for… (more)

Subjects/Keywords: quorum sensing; computational biology; flagellated bacteria; quorum quenching; chemotaxis

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Leaman, E. J. (2016). An Experimentally-validated Agent-based Model to Study the Emergent Behavior of Bacterial Communities. (Masters Thesis). Virginia Tech. Retrieved from http://hdl.handle.net/10919/78072

Chicago Manual of Style (16^th Edition):

Leaman, Eric Joshua. “An Experimentally-validated Agent-based Model to Study the Emergent Behavior of Bacterial Communities.” 2016. Masters Thesis, Virginia Tech. Accessed January 27, 2021. http://hdl.handle.net/10919/78072.

MLA Handbook (7^th Edition):

Leaman, Eric Joshua. “An Experimentally-validated Agent-based Model to Study the Emergent Behavior of Bacterial Communities.” 2016. Web. 27 Jan 2021.

Vancouver:

Leaman EJ. An Experimentally-validated Agent-based Model to Study the Emergent Behavior of Bacterial Communities. [Internet] [Masters thesis]. Virginia Tech; 2016. [cited 2021 Jan 27]. Available from: http://hdl.handle.net/10919/78072.

Council of Science Editors:

Leaman EJ. An Experimentally-validated Agent-based Model to Study the Emergent Behavior of Bacterial Communities. [Masters Thesis]. Virginia Tech; 2016. Available from: http://hdl.handle.net/10919/78072

5. Christiaen, Steven. Exploring the role of quorum sensing and quorum sensing inhibition in the biotherapeutic potential of bacteria.

Degree: 2014, Ghent University

URL: http://hdl.handle.net/1854/LU-4232258

► The involvement of quorum sensing (QS) in several pathogenic bacteria to control biofilm formation and the expression of their virulence factors makes interfering with QS… (more)

Subjects/Keywords: Medicine and Health Sciences; quorum sensing; biotherapeutics; quorum quenching; probiotics

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Christiaen, S. (2014). Exploring the role of quorum sensing and quorum sensing inhibition in the biotherapeutic potential of bacteria. (Thesis). Ghent University. Retrieved from http://hdl.handle.net/1854/LU-4232258

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Christiaen, Steven. “Exploring the role of quorum sensing and quorum sensing inhibition in the biotherapeutic potential of bacteria.” 2014. Thesis, Ghent University. Accessed January 27, 2021. http://hdl.handle.net/1854/LU-4232258.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Christiaen, Steven. “Exploring the role of quorum sensing and quorum sensing inhibition in the biotherapeutic potential of bacteria.” 2014. Web. 27 Jan 2021.

Vancouver:

Christiaen S. Exploring the role of quorum sensing and quorum sensing inhibition in the biotherapeutic potential of bacteria. [Internet] [Thesis]. Ghent University; 2014. [cited 2021 Jan 27]. Available from: http://hdl.handle.net/1854/LU-4232258.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Christiaen S. Exploring the role of quorum sensing and quorum sensing inhibition in the biotherapeutic potential of bacteria. [Thesis]. Ghent University; 2014. Available from: http://hdl.handle.net/1854/LU-4232258

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

6. Chane, Andrea. Régulation, expression in situ et biostimulation de l'activité quorum-quenching d'un agent de biocontrôle : Rhodococcus erythropolis : Regulation, in situ expression and biostimulation of the quorum-quenching activity of a biocontrol agent : Rhodococcus erythropolis.

Degree: Docteur es, Aspects moleculaires et cellulaires de la biologie, 2018, Normandie

URL: http://www.theses.fr/2018NORMR031

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Le biocontrôle est défini comme un ensemble de méthodes de protection des végétaux par l’utilisation de mécanismes naturels. Son principe repose sur la gestion des… (more)

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Le biocontrôle est défini comme un ensemble de méthodes de protection des végétaux par l’utilisation de mécanismes naturels. Son principe repose sur la gestion des équilibres des populations d’agresseurs plutôt que sur leur éradication. La protection des cultures de la pomme de terre Solanum tuberosum contre les bactéries pectinolytiques (Dickeya et Pectobacterium) a été précédemment proposée comme une application du biocontrôle. Il s’agit ici de perturber (quencher) la communication quorum-sensing (QS) utilisée par l’agent pathogène pour coordonner son attaque et sa virulence. Afin d’optimiser cette méthode de lutte par quorum-quenching (QQ) et d’en contrôler l’efficacité, nous avons étudié la voiecatabolique des -lactones d’un agent de biocontrôle, la bactérie Rhodococcus erythropolis. Cette voie est impliquée dans la dégradation des signaux N-acyl-homoserine lactones du pathogène. Nous avons d’abord étudié le rôle du répresseur QsdR ainsi que la régulation transcriptionnelle de l’opéron qsd impliqué dans la dégradation des signaux. La compréhension de cette régulation a permis de générer des biosenseurs capables de monitorer les activités QS du pathogène et QQ du protecteur. Sous microscopie confocale à balayage laser, ces outils ont apporté des preuves visuelles du rôle et du lien entre ces deux activités dans les tissus du tubercule. Enfin, la faible spécificité du répresseur QsdR pour ses ligands, apermis de proposer la -caprolactone, un analogue structural des signaux de QS, comme inducteur de l’opéron qsd. Dans l’ensemble, ces travaux permettent d’approfondir nos connaissances sur le rôle et le fonctionnement du QQ chez R. erythropolis. Ils permettent aussi d’envisager le contrôle de la maladie via un agent dont l’activité de QQ pourra être biostimulée par des lactones peu coûteuses lors de la formulation puis de l’épandage aux champs.

Biocontrol is defined as a set of plant protection methods through the use of natural mechanisms. Its principle involves the control of populations of aggressors rather than their eradication. The protection of the potato Solanum tuberosum against soft-rot bacteria (Dickeya and Pectobacterium) has been previously proposed as an application of biocontrol. This involves disturbing the quorum-sensing (QS) communication used by the pathogen to coordinate its attack and virulence. In order to optimize this quorum-quenching (QQ) biocontrol method and to control its effectiveness, we have studied the catabolic pathway of -lactones of a biocontrol agent, the Rhodococcus erythropolis bacterium. This pathway is involved in the degradation of the pathogen N-acyl-homoserine lactones signals. We firststudied the role of the QsdR repressor as well as the transcriptional regulation of the qsd operon involved in signal degradation. The understanding of this regulation has made it possible to generate biosensors capable of monitoring the QS of the pathogen and QQ of the protector. Under confocal laser scanning microscopy, these tools provided visual evidence of the role and link between…

Advisors/Committee Members: Latour, Xavier (thesis director).

Subjects/Keywords: Lutte biologique; Biocontrôle; Quorum-sensing; Quorum-quenching; Anti-virulence; Lactones; Pomme de terre; Biocontrol; Quorum-sensing; Quorum-quenching; Anti-virulence; Lactones; Potato; 632.9

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Chane, A. (2018). Régulation, expression in situ et biostimulation de l'activité quorum-quenching d'un agent de biocontrôle : Rhodococcus erythropolis : Regulation, in situ expression and biostimulation of the quorum-quenching activity of a biocontrol agent : Rhodococcus erythropolis. (Doctoral Dissertation). Normandie. Retrieved from http://www.theses.fr/2018NORMR031

Chicago Manual of Style (16^th Edition):

Chane, Andrea. “Régulation, expression in situ et biostimulation de l'activité quorum-quenching d'un agent de biocontrôle : Rhodococcus erythropolis : Regulation, in situ expression and biostimulation of the quorum-quenching activity of a biocontrol agent : Rhodococcus erythropolis.” 2018. Doctoral Dissertation, Normandie. Accessed January 27, 2021. http://www.theses.fr/2018NORMR031.

MLA Handbook (7^th Edition):

Chane, Andrea. “Régulation, expression in situ et biostimulation de l'activité quorum-quenching d'un agent de biocontrôle : Rhodococcus erythropolis : Regulation, in situ expression and biostimulation of the quorum-quenching activity of a biocontrol agent : Rhodococcus erythropolis.” 2018. Web. 27 Jan 2021.

Vancouver:

Chane A. Régulation, expression in situ et biostimulation de l'activité quorum-quenching d'un agent de biocontrôle : Rhodococcus erythropolis : Regulation, in situ expression and biostimulation of the quorum-quenching activity of a biocontrol agent : Rhodococcus erythropolis. [Internet] [Doctoral dissertation]. Normandie; 2018. [cited 2021 Jan 27]. Available from: http://www.theses.fr/2018NORMR031.

Council of Science Editors:

Chane A. Régulation, expression in situ et biostimulation de l'activité quorum-quenching d'un agent de biocontrôle : Rhodococcus erythropolis : Regulation, in situ expression and biostimulation of the quorum-quenching activity of a biocontrol agent : Rhodococcus erythropolis. [Doctoral Dissertation]. Normandie; 2018. Available from: http://www.theses.fr/2018NORMR031

7. WANG YUN. APPLICATION OF QUORUM QUENCHING TO CONTROL MICROBIAL BIOFOULING IN MEMBRANE BIOREACTORS.

Degree: 2016, National University of Singapore

URL: http://scholarbank.nus.edu.sg/handle/10635/126072

Subjects/Keywords: Quorum sensing; quorum quenching; MBR; motility; EMMC; biofouling

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

YUN, W. (2016). APPLICATION OF QUORUM QUENCHING TO CONTROL MICROBIAL BIOFOULING IN MEMBRANE BIOREACTORS. (Thesis). National University of Singapore. Retrieved from http://scholarbank.nus.edu.sg/handle/10635/126072

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

YUN, WANG. “APPLICATION OF QUORUM QUENCHING TO CONTROL MICROBIAL BIOFOULING IN MEMBRANE BIOREACTORS.” 2016. Thesis, National University of Singapore. Accessed January 27, 2021. http://scholarbank.nus.edu.sg/handle/10635/126072.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

YUN, WANG. “APPLICATION OF QUORUM QUENCHING TO CONTROL MICROBIAL BIOFOULING IN MEMBRANE BIOREACTORS.” 2016. Web. 27 Jan 2021.

Vancouver:

YUN W. APPLICATION OF QUORUM QUENCHING TO CONTROL MICROBIAL BIOFOULING IN MEMBRANE BIOREACTORS. [Internet] [Thesis]. National University of Singapore; 2016. [cited 2021 Jan 27]. Available from: http://scholarbank.nus.edu.sg/handle/10635/126072.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

YUN W. APPLICATION OF QUORUM QUENCHING TO CONTROL MICROBIAL BIOFOULING IN MEMBRANE BIOREACTORS. [Thesis]. National University of Singapore; 2016. Available from: http://scholarbank.nus.edu.sg/handle/10635/126072

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Maryland

8. Roy, Varnika. ALTERING THE AI-2 MEDIATED QUORUM SENSING CIRCUITRY TO QUENCH BACTERIAL COMMUNICATION NETWORKS.

Degree: Molecular and Cell Biology, 2011, University of Maryland

URL: http://hdl.handle.net/1903/11709

► The emergence of antibiotic resistant bacteria poses a global threat to human health and has been classified as a clinical super-challenge of the 21st century.… (more)

▼ The emergence of antibiotic resistant bacteria poses a global threat to human health and has been classified as a clinical super-challenge of the 21st century. This has necessitated research on new antimicrobials that inhibit bacterial virulence by mechanisms other than those that target bacterial growth or viability. Such approaches have been reported to pose less evolutionary pressure on bacteria to evolve and become resistant to antibiotics. Bacterial cell-cell communication, termed quorum sensing (QS), is mediated by signatures of small molecules. QS via these small molecules has been linked to numerous undesirable bacterial phenotypes such as biofilm formation, onset of pathogenicity, triggering of virulence genes etc. The small signaling molecules represent targets for intercepting bacterial communication (and their resultant undesirable phenotypes). We have devised two strategies that interrupt bacterial communication in multispecies bacterial cultures by targeting the interspecies signaling molecule autoinducer-2 (AI-2), which is produced or recognized by over 70 species of bacteria. Our first approach is to bring the native intracellular AI-2 signal processing mechanisms to the extracellular surroundings to quench the QS response of bacteria. Specifically we deliver the Escherichia coli AI-2 kinase, LsrK, to E. coli populations ex vivo and phosphorylate and degrade the extracellular AI-2. This significantly attenuates the native QS response in E. coli. Similar results are obtained in a tri-species synthetic ecosystem comprising E. coli, Salmonella typhimurium and Vibrio harveyi. In our second quenching strategy, we explore a panel of small synthetic molecules that are analogs of AI-2 (C1-alkyl analogs). The analogs are observed to cause species-specific and cross-species quorum quenching in the tri-species synthetic ecosystems of the aforementioned strains. Some of the AI-2 analogs quench pyocyanin (toxin production) in the opportunistic pathogen Pseudomonas aeruginosa. Based on these observations, I used analog cocktails to quench QS en masse in assembled synthetic ecosystems. Finally, I tested the efficiency of the analogs in quenching pathogenic phenotypes such as biofilm formation in E. coli. The analogs inhibit biofilm formation and act in concert with antibiotics to reduce biofilm formation even further. Our results suggest entirely new modalities for interrupting or tailoring the networks of communication among bacteria and identifying drug targets to develop the next generation of antimicrobials based on QS. Advisors/Committee Members: Bentley, William E (advisor).

Subjects/Keywords: Molecular Biology; Biomedical Engineering; Biochemistry; AI-2; AI-2 Analogs; Cell-Cell Communication; LsrK; Quorum Quenching; Quorum Sensing

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Roy, V. (2011). ALTERING THE AI-2 MEDIATED QUORUM SENSING CIRCUITRY TO QUENCH BACTERIAL COMMUNICATION NETWORKS. (Thesis). University of Maryland. Retrieved from http://hdl.handle.net/1903/11709

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Roy, Varnika. “ALTERING THE AI-2 MEDIATED QUORUM SENSING CIRCUITRY TO QUENCH BACTERIAL COMMUNICATION NETWORKS.” 2011. Thesis, University of Maryland. Accessed January 27, 2021. http://hdl.handle.net/1903/11709.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Roy, Varnika. “ALTERING THE AI-2 MEDIATED QUORUM SENSING CIRCUITRY TO QUENCH BACTERIAL COMMUNICATION NETWORKS.” 2011. Web. 27 Jan 2021.

Vancouver:

Roy V. ALTERING THE AI-2 MEDIATED QUORUM SENSING CIRCUITRY TO QUENCH BACTERIAL COMMUNICATION NETWORKS. [Internet] [Thesis]. University of Maryland; 2011. [cited 2021 Jan 27]. Available from: http://hdl.handle.net/1903/11709.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Roy V. ALTERING THE AI-2 MEDIATED QUORUM SENSING CIRCUITRY TO QUENCH BACTERIAL COMMUNICATION NETWORKS. [Thesis]. University of Maryland; 2011. Available from: http://hdl.handle.net/1903/11709

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Texas – Austin

9. Clevenger, Kenneth David. Investigation and engineering of PvdQ, a Pseudomonas aeruginosa enzyme at the nexus of quorum sensing and iron uptake pathways.

Degree: PhD, Biochemistry, 2014, University of Texas – Austin

URL: http://hdl.handle.net/2152/32902

► The gram-negative human pathogen Pseudomonas aeruginosa is a widespread global health concern. Two key pathways of P. aeruginosa that are involved in its infection and/or… (more)

▼ The gram-negative human pathogen Pseudomonas aeruginosa is a widespread global health concern. Two key pathways of P. aeruginosa that are involved in its infection and/or survival within mammalian hosts are the pyoverdine biosynthetic pathway, which is necessary for iron acquisition, and the N-acyl-homoserine lactone (AHL) quorum sensing (QS) system, which is necessary for P. aeruginosa virulence and infection. The P. aeruginosa enzyme PvdQ is unique in that it is proposed to play roles in both these pathways. To investigate the role of PvdQ in P. aeruginosa, the enzyme’s in vitro substrate profile was characterized and found to favor substrates with unsubstituted myristic acyl groups, supporting a role for PvdQ in pyoverdine biosynthesis, but challenging its proposed role in regulating P. aeruginosa QS. Knowledge of PvdQ’s substrate preference and mechanism enabled rational inhibitor design, leading to the discovery of long chain (≥ 10 carbons) n-alkylboronic acids as highly potent transition state analog inhibitors of PvdQ, with 1-tridecylboronic acid having a Ki of 200 ± 40 pM and having the ability to recapitulate the growth phenotype of a pvdQ transposon insertion strain of P. aeruginosa under certain experimental conditions. Investigation of PvdQ inhibition by alkylboronic acids was extended to short- and medium-chain alkylboronic acids and revealed an eight order of magnitude span of affinity that is linearly related to alkyl chain length. Through structural studies, short-chain alkylboronic acids (≤ 4 carbons) were found to adopt an alternate binding conformation relative to other alkylboronic acids, which suggests a mechanism by which PvdQ excludes short substrates such as P. aeruginosa QS molecule N-butyroyl-homoserine lactone. Finally, a circular permutation of PvdQ (cpPvdQ) was designed and constructed in order to uncouple the catalytic and self-processing activities of PvdQ and to facilitate the production of mutants that affect catalysis and specificity. The cpPvdQ monomer recapitulates the catalytic and structural features of PvdQ. A catalytically impaired cpPvdQ mutant that is shown to maintain affinity for PvdQ’s native substrate, the pyoverdine precursor, but does not hydrolyze the substrate, has been produced and will be a valuable tool for future efforts to trap a PvdQ derivative in complex with the pyoverdine precursor. Advisors/Committee Members: Fast, Walter L. (advisor), Whitman, Christian P (committee member), Whiteley, Marvin (committee member), Keatinge-Clay, Adrian (committee member), Zhang, Jessie Y (committee member).

Subjects/Keywords: NTN-hydrolase; PvdQ; Siderophore; Pyoverdine; NRPS; Quorum-sensing; Quorum-quenching; Alkylboronic acid; Boronic acid; Iron limited growth; Pseudomonas aeruginosa

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Clevenger, K. D. (2014). Investigation and engineering of PvdQ, a Pseudomonas aeruginosa enzyme at the nexus of quorum sensing and iron uptake pathways. (Doctoral Dissertation). University of Texas – Austin. Retrieved from http://hdl.handle.net/2152/32902

Chicago Manual of Style (16^th Edition):

Clevenger, Kenneth David. “Investigation and engineering of PvdQ, a Pseudomonas aeruginosa enzyme at the nexus of quorum sensing and iron uptake pathways.” 2014. Doctoral Dissertation, University of Texas – Austin. Accessed January 27, 2021. http://hdl.handle.net/2152/32902.

MLA Handbook (7^th Edition):

Clevenger, Kenneth David. “Investigation and engineering of PvdQ, a Pseudomonas aeruginosa enzyme at the nexus of quorum sensing and iron uptake pathways.” 2014. Web. 27 Jan 2021.

Vancouver:

Clevenger KD. Investigation and engineering of PvdQ, a Pseudomonas aeruginosa enzyme at the nexus of quorum sensing and iron uptake pathways. [Internet] [Doctoral dissertation]. University of Texas – Austin; 2014. [cited 2021 Jan 27]. Available from: http://hdl.handle.net/2152/32902.

Council of Science Editors:

Clevenger KD. Investigation and engineering of PvdQ, a Pseudomonas aeruginosa enzyme at the nexus of quorum sensing and iron uptake pathways. [Doctoral Dissertation]. University of Texas – Austin; 2014. Available from: http://hdl.handle.net/2152/32902

10. Dussan Garzon , Jenny. Identification of a Possible Novel N-Acyl Homoserin Lactonase from Lysinibacillus sphaericus and Geobacillus sp. Strains .

Degree: 2015, Universidad de los Andes

URL: http://documentodegrado.uniandes.edu.co/documentos/200921677_fecha_2015_01_17_hora_20_41_39_parte_1.pdf

► Recientemente gran cantidad de publicaciones sobre quorum quenching se enfocan en el descubrimiento y caracterización de nuevas AHL-lactonasas debido a su potencial aplicación en el… (more)

Subjects/Keywords: Quorum sensing; Quorum quenching; Lysinibacillus sphaericus; AHL-lactonase; Sporulated bacilli

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Dussan Garzon , J. (2015). Identification of a Possible Novel N-Acyl Homoserin Lactonase from Lysinibacillus sphaericus and Geobacillus sp. Strains . (Thesis). Universidad de los Andes. Retrieved from http://documentodegrado.uniandes.edu.co/documentos/200921677_fecha_2015_01_17_hora_20_41_39_parte_1.pdf

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Dussan Garzon , Jenny. “Identification of a Possible Novel N-Acyl Homoserin Lactonase from Lysinibacillus sphaericus and Geobacillus sp. Strains .” 2015. Thesis, Universidad de los Andes. Accessed January 27, 2021. http://documentodegrado.uniandes.edu.co/documentos/200921677_fecha_2015_01_17_hora_20_41_39_parte_1.pdf.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Dussan Garzon , Jenny. “Identification of a Possible Novel N-Acyl Homoserin Lactonase from Lysinibacillus sphaericus and Geobacillus sp. Strains .” 2015. Web. 27 Jan 2021.

Vancouver:

Dussan Garzon J. Identification of a Possible Novel N-Acyl Homoserin Lactonase from Lysinibacillus sphaericus and Geobacillus sp. Strains . [Internet] [Thesis]. Universidad de los Andes; 2015. [cited 2021 Jan 27]. Available from: http://documentodegrado.uniandes.edu.co/documentos/200921677_fecha_2015_01_17_hora_20_41_39_parte_1.pdf.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Dussan Garzon J. Identification of a Possible Novel N-Acyl Homoserin Lactonase from Lysinibacillus sphaericus and Geobacillus sp. Strains . [Thesis]. Universidad de los Andes; 2015. Available from: http://documentodegrado.uniandes.edu.co/documentos/200921677_fecha_2015_01_17_hora_20_41_39_parte_1.pdf

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

11. Gomez Garzon , Camilo Andres. Identification of a Possible Novel N-Acyl Homoserin Lactonase from Lysinibacillus sphaericus and Geobacillus sp. Strains .

Degree: 2015, Universidad de los Andes

URL: http://documentodegrado.uniandes.edu.co/documentos/200921677_fecha_2015_01_17_hora_20_41_39_parte_1.pdf

► Recientemente gran cantidad de publicaciones sobre quorum quenching se enfocan en el descubrimiento y caracterización de nuevas AHL-lactonasas debido a su potencial aplicación en el… (more)

Subjects/Keywords: Quorum sensing; Quorum quenching; Lysinibacillus sphaericus; AHL-lactonase; Sporulated bacilli

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Gomez Garzon , C. A. (2015). Identification of a Possible Novel N-Acyl Homoserin Lactonase from Lysinibacillus sphaericus and Geobacillus sp. Strains . (Thesis). Universidad de los Andes. Retrieved from http://documentodegrado.uniandes.edu.co/documentos/200921677_fecha_2015_01_17_hora_20_41_39_parte_1.pdf

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Gomez Garzon , Camilo Andres. “Identification of a Possible Novel N-Acyl Homoserin Lactonase from Lysinibacillus sphaericus and Geobacillus sp. Strains .” 2015. Thesis, Universidad de los Andes. Accessed January 27, 2021. http://documentodegrado.uniandes.edu.co/documentos/200921677_fecha_2015_01_17_hora_20_41_39_parte_1.pdf.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Gomez Garzon , Camilo Andres. “Identification of a Possible Novel N-Acyl Homoserin Lactonase from Lysinibacillus sphaericus and Geobacillus sp. Strains .” 2015. Web. 27 Jan 2021.

Vancouver:

Gomez Garzon CA. Identification of a Possible Novel N-Acyl Homoserin Lactonase from Lysinibacillus sphaericus and Geobacillus sp. Strains . [Internet] [Thesis]. Universidad de los Andes; 2015. [cited 2021 Jan 27]. Available from: http://documentodegrado.uniandes.edu.co/documentos/200921677_fecha_2015_01_17_hora_20_41_39_parte_1.pdf.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Gomez Garzon CA. Identification of a Possible Novel N-Acyl Homoserin Lactonase from Lysinibacillus sphaericus and Geobacillus sp. Strains . [Thesis]. Universidad de los Andes; 2015. Available from: http://documentodegrado.uniandes.edu.co/documentos/200921677_fecha_2015_01_17_hora_20_41_39_parte_1.pdf

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

12. Giraldo Perez , Daniel. Modelación determinista de un sistema de represiladores acoplados mediante un sistema de quorum sensing con la implementación de una lactonasa como mecanismo de reinicio.

Degree: 2013, Universidad de los Andes

URL: http://documentodegrado.uniandes.edu.co/documentos/200911848_fecha_2014_08_11_hora_17_33_43_parte_1.pdf

► Diversos esfuerzos han sido realizados para el desarrollo de circuitos genéticos que cumplan funciones específicas como puertas lógicas, generadores de pulsos y osciladores. Sin embargo,… (more)

Subjects/Keywords: quorum sensing; biologia sintetica; modelo determinista; quorum quenching; represilador; repressilator

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APA (6^th Edition):

Giraldo Perez , D. (2013). Modelación determinista de un sistema de represiladores acoplados mediante un sistema de quorum sensing con la implementación de una lactonasa como mecanismo de reinicio. (Thesis). Universidad de los Andes. Retrieved from http://documentodegrado.uniandes.edu.co/documentos/200911848_fecha_2014_08_11_hora_17_33_43_parte_1.pdf

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Giraldo Perez , Daniel. “Modelación determinista de un sistema de represiladores acoplados mediante un sistema de quorum sensing con la implementación de una lactonasa como mecanismo de reinicio. ” 2013. Thesis, Universidad de los Andes. Accessed January 27, 2021. http://documentodegrado.uniandes.edu.co/documentos/200911848_fecha_2014_08_11_hora_17_33_43_parte_1.pdf.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Giraldo Perez , Daniel. “Modelación determinista de un sistema de represiladores acoplados mediante un sistema de quorum sensing con la implementación de una lactonasa como mecanismo de reinicio. ” 2013. Web. 27 Jan 2021.

Vancouver:

Giraldo Perez D. Modelación determinista de un sistema de represiladores acoplados mediante un sistema de quorum sensing con la implementación de una lactonasa como mecanismo de reinicio. [Internet] [Thesis]. Universidad de los Andes; 2013. [cited 2021 Jan 27]. Available from: http://documentodegrado.uniandes.edu.co/documentos/200911848_fecha_2014_08_11_hora_17_33_43_parte_1.pdf.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Giraldo Perez D. Modelación determinista de un sistema de represiladores acoplados mediante un sistema de quorum sensing con la implementación de una lactonasa como mecanismo de reinicio. [Thesis]. Universidad de los Andes; 2013. Available from: http://documentodegrado.uniandes.edu.co/documentos/200911848_fecha_2014_08_11_hora_17_33_43_parte_1.pdf

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Otago

13. Wiggins, Thomas James. Disrupting Pseudomonas aeruginosa quorum sensing .

Degree: University of Otago

URL: http://hdl.handle.net/10523/9651

► Many clinically relevant bacteria use a population-dependent communication network - known as quorum sensing - to regulate the processes essential for the establishment of infections.… (more)

▼ Many clinically relevant bacteria use a population-dependent communication network - known as quorum sensing - to regulate the processes essential for the establishment of infections. Quorum sensing enables pathogens to restrict the expression of specific genes until a population capable of overwhelming the host defences is reached. Due to its importance in bacterial virulence and biofilm formation, the disruption of this system (quorum quenching) is a promising strategy to treat bacterial infections. Quorum sensing involves the synthesis, secretion, and detection of chemical signals, termed autoinducers. Disrupting any one of these steps can inhibit quorum sensing. This project investigated two different approaches for quorum quenching: the enzymatic degradation of autoinducers, and the inhibition of autoinducer synthesis. The model system used to investigate the effectiveness of these approaches was Pseudomonas aeruginosa PAO1. P. aeruginosa is an ubiquitous environmental bacterium and opportunistic pathogen. The first approach investigated the degradation of the two N-acyl-homoserine lactone (AHL) autoinducers produced by P. aeruginosa; N-butyryl-L-homoserine lactone (C4-HSL) and N-3-oxo-dodecanoyl-L-homoserine lactone (3-oxo-C12-HSL). The P. aeruginosa AHL acylase HacB was selected as a scaffold for mutagenesis to introduce acylase activity towards C4-HSL and generate a broad specificity acylase. Although mutagenesis of HacB successfully introduced C4-HSL activity, the formation of P. aeruginosa PAO1 biofilms was not disrupted. Therefore, an alternative quorum quenching method that focused on inhibiting AHL production was investigated. During the synthesis of AHLs, the by-product 5'-methylthioadenosine (MTA) is also generated. MTA is rapidly recycled to prevent product inhibition. To determine if this pathway was a viable target for drug development, the effects of deleting it in P. aeruginosa PAO1 were characterised. Deletion of the MTA recycling pathway reduced C4-HSL accumulation, pyocyanin production, and swarming motility, however 3-oxo-C12-HSL accumulation and biofilm formation were not affected. The work presented here shows that targeting P. aeruginosa quorum sensing is a viable approach to reducing virulence, and provides the foundation for further development of effective quorum quenching methods. Advisors/Committee Members: Lamont, Iain L (advisor).

Subjects/Keywords: New Zealand; Psuedomonas; aeruginosa; quorum; sensing; quenching; AHL; acylase

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Wiggins, T. J. (n.d.). Disrupting Pseudomonas aeruginosa quorum sensing . (Doctoral Dissertation). University of Otago. Retrieved from http://hdl.handle.net/10523/9651

Note: this citation may be lacking information needed for this citation format:
No year of publication.

Chicago Manual of Style (16^th Edition):

Wiggins, Thomas James. “Disrupting Pseudomonas aeruginosa quorum sensing .” Doctoral Dissertation, University of Otago. Accessed January 27, 2021. http://hdl.handle.net/10523/9651.

Note: this citation may be lacking information needed for this citation format:
No year of publication.

MLA Handbook (7^th Edition):

Wiggins, Thomas James. “Disrupting Pseudomonas aeruginosa quorum sensing .” Web. 27 Jan 2021.

Note: this citation may be lacking information needed for this citation format:
No year of publication.

Vancouver:

Wiggins TJ. Disrupting Pseudomonas aeruginosa quorum sensing . [Internet] [Doctoral dissertation]. University of Otago; [cited 2021 Jan 27]. Available from: http://hdl.handle.net/10523/9651.

Note: this citation may be lacking information needed for this citation format:
No year of publication.

Council of Science Editors:

Wiggins TJ. Disrupting Pseudomonas aeruginosa quorum sensing . [Doctoral Dissertation]. University of Otago; Available from: http://hdl.handle.net/10523/9651

Note: this citation may be lacking information needed for this citation format:
No year of publication.

Univerzitet u Beogradu

14. Janakiev, Tamara, 1989-, 31844199. Mikrobiom šljive (Prunus domestica L.) i potencijal odabranih izolata za biokontrolu patogena šljive.

Degree: Biološki fakultet, 2020, Univerzitet u Beogradu

URL: https://fedorabg.bg.ac.rs/fedora/get/o:22024/bdef:Content/get

►

Biologija - Mikrobiologija / Biology - Microbiology

Šljiva (Prunus domestica L.) ima izuzetan značaj kao voćarska kultura u Srbiji i tradicionalno se prerađuje u rakiju… (more)

▼

Biologija - Mikrobiologija / Biology - Microbiology

Šljiva (Prunus domestica L.) ima izuzetan značaj kao voćarska kultura u Srbiji i tradicionalno se prerađuje u rakiju šljivovicu. S obzirom da je godišnji prinos šljive ugrožen različitim bolestima, naročito izazivačima bakterioznog raka i mrke truleži neophodno je naći efikasne agense za njihovo suzbijanje, a naročito alternativu upotrebi hemijskih pesticida. U ovom radu je prvi put analiziran mikrobiom filosfere četiri sorte šljive (Čačanska lepotica, Čačanska rodna, Požegača i Ranka), obuhvatajući ranu (maj) i kasnu fenološku fazu (juli) razvića ploda. Indeksi alfa i beta diverziteta su pokazali da su zajednice bile heterogene i da je njihov sastav zavisio od sezone uzorkovanja i sorti šljive. U ukupnoj bakterijskoj zajednici je metagenomskim pristupom utvrđeno da su predstavnici razdela Proteobacteria bili najdominantniji, a najzastupljenije vrste su bile iz rodova: Methylobacterium, Sphingomonas i Hymenobacter. Tradicionalnim kultivabilnim pristupom i analizom sekvenci gena za 16S rRNK identifikovane su Pseudomonas syringae i Pseudomonas graminis kao najdominantnije vrste. Analiza mikobioma je ukazala na dominatnu zastupljenost predstavnika razdela Ascomycota. Vrste iz rodova Aureobasidium i Cryptococcus su bile najučestalije u maju, dok su u julu predstavnici različitih rodova dominirali na sortama šljive (Cryptococcus, Metschnikowia, Fusarium i Hanseniaspora). U okviru kolekcije kultivabilnih gljiva, identifikovano je 11 izolata Monilinia laxa na osnovu filogenetske analize sekvenci ITS regiona. Odabrani izolati M. laxa su prouzrokovali tipične simptome na ubranim plodovima sorti Požegača i Čačanska rodna, a testiranjem potencijalnih bakterijskih patogena su izdvojeni izolati P. syringae ČL2/2 i P7/16_2, koji su izazivali nekroze na ubranim listovima šljive svih ispitivanih sorti. Analizom antagonističkog potencijala kolekcije bakterija i kvasaca poreklom sa sve četiri sorte je izdvojen izolat Bacillus thuringiensis R3/3 sa snažnom antibakterijskom aktivnošću protiv autohtonih i referentnih fitopatogena...

Advisors/Committee Members: Berić, Tanja, 1974-, 12870759.

Subjects/Keywords: microbial diversity; phyllosphere; plum; NGS; Monilinia laxa; Bacillus thuringiensis; Pseudomonas synxantha; antagonism; quorum quenching; phenazines; biological control

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APA (6^th Edition):

Janakiev, Tamara, 1989-, 3. (2020). Mikrobiom šljive (Prunus domestica L.) i potencijal odabranih izolata za biokontrolu patogena šljive. (Thesis). Univerzitet u Beogradu. Retrieved from https://fedorabg.bg.ac.rs/fedora/get/o:22024/bdef:Content/get

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Janakiev, Tamara, 1989-, 31844199. “Mikrobiom šljive (Prunus domestica L.) i potencijal odabranih izolata za biokontrolu patogena šljive.” 2020. Thesis, Univerzitet u Beogradu. Accessed January 27, 2021. https://fedorabg.bg.ac.rs/fedora/get/o:22024/bdef:Content/get.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Janakiev, Tamara, 1989-, 31844199. “Mikrobiom šljive (Prunus domestica L.) i potencijal odabranih izolata za biokontrolu patogena šljive.” 2020. Web. 27 Jan 2021.

Vancouver:

Janakiev, Tamara, 1989- 3. Mikrobiom šljive (Prunus domestica L.) i potencijal odabranih izolata za biokontrolu patogena šljive. [Internet] [Thesis]. Univerzitet u Beogradu; 2020. [cited 2021 Jan 27]. Available from: https://fedorabg.bg.ac.rs/fedora/get/o:22024/bdef:Content/get.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Janakiev, Tamara, 1989- 3. Mikrobiom šljive (Prunus domestica L.) i potencijal odabranih izolata za biokontrolu patogena šljive. [Thesis]. Univerzitet u Beogradu; 2020. Available from: https://fedorabg.bg.ac.rs/fedora/get/o:22024/bdef:Content/get

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

15. Kusari, Parijat. Isolation, identification and characterization of endophytes from Cannabis sativa L. and Radula marginata.

Degree: 2014, Technische Universität Dortmund

URL: http://dx.doi.org/10.17877/DE290R-7295

Subjects/Keywords: Endophytes; Cannabis sativa L.; Quorum quenching; HPLC-HRMS; MALDI-imaging; 660

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Kusari, P. (2014). Isolation, identification and characterization of endophytes from Cannabis sativa L. and Radula marginata. (Doctoral Dissertation). Technische Universität Dortmund. Retrieved from http://dx.doi.org/10.17877/DE290R-7295

Chicago Manual of Style (16^th Edition):

Kusari, Parijat. “Isolation, identification and characterization of endophytes from Cannabis sativa L. and Radula marginata.” 2014. Doctoral Dissertation, Technische Universität Dortmund. Accessed January 27, 2021. http://dx.doi.org/10.17877/DE290R-7295.

MLA Handbook (7^th Edition):

Kusari, Parijat. “Isolation, identification and characterization of endophytes from Cannabis sativa L. and Radula marginata.” 2014. Web. 27 Jan 2021.

Vancouver:

Kusari P. Isolation, identification and characterization of endophytes from Cannabis sativa L. and Radula marginata. [Internet] [Doctoral dissertation]. Technische Universität Dortmund; 2014. [cited 2021 Jan 27]. Available from: http://dx.doi.org/10.17877/DE290R-7295.

Council of Science Editors:

Kusari P. Isolation, identification and characterization of endophytes from Cannabis sativa L. and Radula marginata. [Doctoral Dissertation]. Technische Universität Dortmund; 2014. Available from: http://dx.doi.org/10.17877/DE290R-7295

Universitat Politècnica de Catalunya

16. Ivanova, Kristina Dimitrova. Nanostructured coatings for controlling bacterial biofilms and antibiotic resistance.

Degree: Departament d'Enginyeria Química, 2017, Universitat Politècnica de Catalunya

URL: http://hdl.handle.net/10803/405631

► La rápida aparición de bacterias resistentes a fármacos es uno de los problemas más graves del sistema sanitario convirtiéndose en un gran reto encontrar nuevos… (more)

▼ La rápida aparición de bacterias resistentes a fármacos es uno de los problemas más graves del sistema sanitario convirtiéndose en un gran reto encontrar nuevos antibióticos. Para superar la acción de los antibióticos, las bacterias utilizan diferentes mecanismos de resistencia incluyendo la formación de biopelículas. Las biopelículas son comunidades complejas de células bacterianas unidas por una matriz polimérica comúnmente encontradas en dispositivos médicos invasivos como los catéteres urinarios. Cuando los patógenos adoptan este modo de crecimiento en superficies, evitan eficazmente las defensas inmunitarias y el efecto de los antibióticos, causando infecciones. Esta tesis se centra en el desarrollo de nuevos materiales y recubrimientos nanoestructurados para el control de biopelículas bacterianas y la reducción de su resistencia a antibióticos. Por lo tanto, las enzimas acilasa y amilasa, capaces de degradar los componentes necesarios de las bacterias para formar biopelículas, se combinaron de forma innovadora en nanorecubrimientos híbridos para intervenir en el crecimiento de las biopelículas en dispositivos médicos permanentes. Además, la transformación de agentes antimicrobianos a forma "nano" se utilizó para mejorar su eficacia antibacteriana y superarlos mecanismos de resistencia a fármacos de las bacterias. Estas estrategias ofrecen nuevas perspectivas para el tratamiento de las infecciones relacionadas con biopelículas, limitando la selección y propagación de la resistencia bacteriana. La primera parte de la tesis describe la generación de recubrimientos multicapa de enzimas capaces de interferir con el sistema de comunicación bacteriana, denominado quórum sensing (QS) y prevenir la formación de biopelículas en el superficie de los catéteres urinarios. Esto se consiguió por deposición alternada de acilasa, cargada negativamente, y polietilenimina, cargada de manera opuesta, en la forma de capa a capa (LbL). Los catéteres recubiertos con acilasa inhibieron el proceso QS de bacterias Gram-negativas y redujeron significativamente el crecimiento de biopelículas en los catéteres urinarios en condiciones que imitaban la situación real en pacientes cateterizados. Adicionalmente, la acilasa se combinó con la enzima amilasa en recubrimientos híbridos capaces de interferir con las señales de comunicación entre bacterias y la integridad de la matriz de la biopelícula. El ensamblaje de las dos enzimas en recubrimientos híbridos dio lugar a una inhibición de la formación de biopelículas más fuerte en función de la localización de la acilasa o de la amilasa en la multicapa. Nanorecubrimientos con acilasa en la capa más externa redujo la formación de biopelículas en catéteres de silicona in vitro y en un modelo animal in vivo. La tesis también muestra la eficacia de nanomateriales para el control de biopelículas bacterianas. Las nanoesferas (NSs) antibacterianas, que contienen en su estructura multicapas ensambladas que se liberan bajo demanda, fueron depositados sobre silicona. Aminocelulosa (AC) se transformó primero… Advisors/Committee Members: [email protected] (authoremail), false (authoremailshow), Tzanov, Tzanko (director), true (authorsendemail).

Subjects/Keywords: Enzymes; Layer-by-Layer coatings; Sonochemistry; Nanospheres; Urinary catheters; Quorum quenching; Antibacterial activity; Biofilm prevention; Antibiotic resistance; Àrees temàtiques de la UPC::Enginyeria química; 66

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Ivanova, K. D. (2017). Nanostructured coatings for controlling bacterial biofilms and antibiotic resistance. (Thesis). Universitat Politècnica de Catalunya. Retrieved from http://hdl.handle.net/10803/405631

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Ivanova, Kristina Dimitrova. “Nanostructured coatings for controlling bacterial biofilms and antibiotic resistance.” 2017. Thesis, Universitat Politècnica de Catalunya. Accessed January 27, 2021. http://hdl.handle.net/10803/405631.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Ivanova, Kristina Dimitrova. “Nanostructured coatings for controlling bacterial biofilms and antibiotic resistance.” 2017. Web. 27 Jan 2021.

Vancouver:

Ivanova KD. Nanostructured coatings for controlling bacterial biofilms and antibiotic resistance. [Internet] [Thesis]. Universitat Politècnica de Catalunya; 2017. [cited 2021 Jan 27]. Available from: http://hdl.handle.net/10803/405631.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Ivanova KD. Nanostructured coatings for controlling bacterial biofilms and antibiotic resistance. [Thesis]. Universitat Politècnica de Catalunya; 2017. Available from: http://hdl.handle.net/10803/405631

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

17. WANG CHAO. Genetic and signal regulation of quorum sensing in Agrobacterium tumefaciens.

Degree: 2007, National University of Singapore

URL: http://scholarbank.nus.edu.sg/handle/10635/12969

Subjects/Keywords: quorum quenching; semialdehyde acid; signal degradation; crown gall; stress response; ppGpp

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

CHAO, W. (2007). Genetic and signal regulation of quorum sensing in Agrobacterium tumefaciens. (Thesis). National University of Singapore. Retrieved from http://scholarbank.nus.edu.sg/handle/10635/12969

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

CHAO, WANG. “Genetic and signal regulation of quorum sensing in Agrobacterium tumefaciens.” 2007. Thesis, National University of Singapore. Accessed January 27, 2021. http://scholarbank.nus.edu.sg/handle/10635/12969.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

CHAO, WANG. “Genetic and signal regulation of quorum sensing in Agrobacterium tumefaciens.” 2007. Web. 27 Jan 2021.

Vancouver:

CHAO W. Genetic and signal regulation of quorum sensing in Agrobacterium tumefaciens. [Internet] [Thesis]. National University of Singapore; 2007. [cited 2021 Jan 27]. Available from: http://scholarbank.nus.edu.sg/handle/10635/12969.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

CHAO W. Genetic and signal regulation of quorum sensing in Agrobacterium tumefaciens. [Thesis]. National University of Singapore; 2007. Available from: http://scholarbank.nus.edu.sg/handle/10635/12969

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

18. TAY SONG BUCK, TERENCE. SYNTHETIC ENZYMOLOGY APPLICATIONS IN THERAPEUTICS AND BIOREMEDIATION.

Degree: 2017, National University of Singapore

URL: http://scholarbank.nus.edu.sg/handle/10635/137922

Subjects/Keywords: Quorum-quenching; metal-independent enzyme; Chromobacterium violaceum; bioremediation; gold bioleaching; electronic waste

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

TAY SONG BUCK, T. (2017). SYNTHETIC ENZYMOLOGY APPLICATIONS IN THERAPEUTICS AND BIOREMEDIATION. (Thesis). National University of Singapore. Retrieved from http://scholarbank.nus.edu.sg/handle/10635/137922

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

TAY SONG BUCK, TERENCE. “SYNTHETIC ENZYMOLOGY APPLICATIONS IN THERAPEUTICS AND BIOREMEDIATION.” 2017. Thesis, National University of Singapore. Accessed January 27, 2021. http://scholarbank.nus.edu.sg/handle/10635/137922.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

TAY SONG BUCK, TERENCE. “SYNTHETIC ENZYMOLOGY APPLICATIONS IN THERAPEUTICS AND BIOREMEDIATION.” 2017. Web. 27 Jan 2021.

Vancouver:

TAY SONG BUCK T. SYNTHETIC ENZYMOLOGY APPLICATIONS IN THERAPEUTICS AND BIOREMEDIATION. [Internet] [Thesis]. National University of Singapore; 2017. [cited 2021 Jan 27]. Available from: http://scholarbank.nus.edu.sg/handle/10635/137922.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

TAY SONG BUCK T. SYNTHETIC ENZYMOLOGY APPLICATIONS IN THERAPEUTICS AND BIOREMEDIATION. [Thesis]. National University of Singapore; 2017. Available from: http://scholarbank.nus.edu.sg/handle/10635/137922

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

19. Momb, Jessica E. An investigation of a quorum-quenching lactonase from Bacillus thuringiensis.

Degree: PhD, Biochemistry, 2009, University of Texas – Austin

URL: http://hdl.handle.net/2152/ETD-UT-2009-12-421

► Gram-negative bacteria use N-acyl homoserine lactones (AHLs) to sense population density and regulate gene expression, including virulent phenotypes. The quorum-quenching AHL lactonase from Bacillus thuringiensis… (more)

▼ Gram-negative bacteria use N-acyl homoserine lactones (AHLs) to sense population density and regulate gene expression, including virulent phenotypes. The quorum-quenching AHL lactonase from Bacillus thuringiensis cleaves the lactone ring of AHLs, disabling this mode of gene regulation. Despite the potential applications of this enzyme as an antibacterial weapon, little was known about it's lactone ring-opening mechanism. As a member of the metallo-beta-lactamase superfamily, AHL lactonase requires two divalent metal ions for catalysis. NMR experiments confirm that these metal ions are also involved in proper enzyme folding. The chemical mechanism of ring opening was explored using isotope incorporation studies, and hydrolysis was determined to proceed via a nucleophilic attack by a solvent-derived hydroxide at the carbonyl of the lactone ring. A transient, kinetically significant metal-leaving group interaction was detected in steady-state kinetic assays with AHL lactonase containing alternative divalent metal ions hydrolyzing a sulfur-containing substrate. High-resolution crystal structures implicated two residues in substrate binding and hydrolysis, Tyr194 and Asp108. Site-directed mutagenesis of these residues followed by steady-state kinetic studies with wild-type and mutant enzymes hydrolyzing a spectrum of AHL substrates revealed that mutations Y194F and D108N significantly affect catalysis. Combining these results allows the proposal of a detailed hydrolytic mechanism. The binding site for the N-acyl hydrophobic moiety was probed using steady-state kinetics with a variety of naturally occurring and non-natural AHL substrates, and these studies indicate that AHL lactonase will accept a broad range of homoserine lactone containing substrates. Crystal structures with AHL substrates and non-hydrolyzable analogs reveal two distinct binding sites for this N-acyl group. Based on the ability of this enzyme to accommodate a variety of substrates, AHL lactonase was shown to have the ability to quench quorum sensing regulated by a newly discovered class of homoserine lactone signal molecules possessing an N-aryl group using a bioassay. Steady-state kinetic studies confirm that this class of signal molecules are indeed substrates for AHL lactonase. Advisors/Committee Members: Fast, Walter L. (advisor), Hoffman, David (committee member), Johnson, Kenneth (committee member), Whitman, Christian (committee member), Zhang, Yan Jessie (committee member).

Subjects/Keywords: Quorum-quenching; Lactonase; Metalloenyzme; N-acyl homoserine lactone

…Bioassay for Quorum Quenching Using the R. palustris Quorum Sensing System… …Bioassay for Quorum Quenching of the R. palustris Quorum Sensing System.....115 Discussion… …purified quorum quenching acylase to cultures of P. aeruginosa resulted in a reduction of… …1 Quorum Sensing in Gramnegative Bacteria… …114 xiii List of Figures Figure 1.1 Structure of the Vibrio fischeri quorum sensing…

10 more images…

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Momb, J. E. (2009). An investigation of a quorum-quenching lactonase from Bacillus thuringiensis. (Doctoral Dissertation). University of Texas – Austin. Retrieved from http://hdl.handle.net/2152/ETD-UT-2009-12-421

Chicago Manual of Style (16^th Edition):

Momb, Jessica E. “An investigation of a quorum-quenching lactonase from Bacillus thuringiensis.” 2009. Doctoral Dissertation, University of Texas – Austin. Accessed January 27, 2021. http://hdl.handle.net/2152/ETD-UT-2009-12-421.

MLA Handbook (7^th Edition):

Momb, Jessica E. “An investigation of a quorum-quenching lactonase from Bacillus thuringiensis.” 2009. Web. 27 Jan 2021.

Vancouver:

Momb JE. An investigation of a quorum-quenching lactonase from Bacillus thuringiensis. [Internet] [Doctoral dissertation]. University of Texas – Austin; 2009. [cited 2021 Jan 27]. Available from: http://hdl.handle.net/2152/ETD-UT-2009-12-421.

Council of Science Editors:

Momb JE. An investigation of a quorum-quenching lactonase from Bacillus thuringiensis. [Doctoral Dissertation]. University of Texas – Austin; 2009. Available from: http://hdl.handle.net/2152/ETD-UT-2009-12-421

University of Otago

20. Murugayah, Shereen Asha. Engineering quorum-quenching enzymes .

Degree: University of Otago

URL: http://hdl.handle.net/10523/8288

► Biofilms are structured communities of bacteria that adhere to surfaces and shield bacteria from hostile surroundings. They are implicated in 75% of all infections in… (more)

Subjects/Keywords: acyl-homoserine lactone acylase; quorum quenching; glutaryl-7-amino-cephalosporanic acid acylase; enzyme engineering; acyl-homoserine lactone; AHL

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Murugayah, S. A. (n.d.). Engineering quorum-quenching enzymes . (Doctoral Dissertation). University of Otago. Retrieved from http://hdl.handle.net/10523/8288

Note: this citation may be lacking information needed for this citation format:
No year of publication.

Chicago Manual of Style (16^th Edition):

Murugayah, Shereen Asha. “Engineering quorum-quenching enzymes .” Doctoral Dissertation, University of Otago. Accessed January 27, 2021. http://hdl.handle.net/10523/8288.

Note: this citation may be lacking information needed for this citation format:
No year of publication.

MLA Handbook (7^th Edition):

Murugayah, Shereen Asha. “Engineering quorum-quenching enzymes .” Web. 27 Jan 2021.

Note: this citation may be lacking information needed for this citation format:
No year of publication.

Vancouver:

Murugayah SA. Engineering quorum-quenching enzymes . [Internet] [Doctoral dissertation]. University of Otago; [cited 2021 Jan 27]. Available from: http://hdl.handle.net/10523/8288.

Note: this citation may be lacking information needed for this citation format:
No year of publication.

Council of Science Editors:

Murugayah SA. Engineering quorum-quenching enzymes . [Doctoral Dissertation]. University of Otago; Available from: http://hdl.handle.net/10523/8288

Note: this citation may be lacking information needed for this citation format:
No year of publication.

21. HUANG SHUJUAN. MEMBRANE FOULING CONTROL IN INDUSTRIAL WASTEWATER TREATMENT.

Degree: 2020, National University of Singapore

URL: https://scholarbank.nus.edu.sg/handle/10635/168230

Subjects/Keywords: Membrane bioreactor; pharmaceutical wastewater; ferric hydroxide; N-acyl homoserine lactone; SPE-LLE; quorum quenching

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

SHUJUAN, H. (2020). MEMBRANE FOULING CONTROL IN INDUSTRIAL WASTEWATER TREATMENT. (Thesis). National University of Singapore. Retrieved from https://scholarbank.nus.edu.sg/handle/10635/168230

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

SHUJUAN, HUANG. “MEMBRANE FOULING CONTROL IN INDUSTRIAL WASTEWATER TREATMENT.” 2020. Thesis, National University of Singapore. Accessed January 27, 2021. https://scholarbank.nus.edu.sg/handle/10635/168230.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

SHUJUAN, HUANG. “MEMBRANE FOULING CONTROL IN INDUSTRIAL WASTEWATER TREATMENT.” 2020. Web. 27 Jan 2021.

Vancouver:

SHUJUAN H. MEMBRANE FOULING CONTROL IN INDUSTRIAL WASTEWATER TREATMENT. [Internet] [Thesis]. National University of Singapore; 2020. [cited 2021 Jan 27]. Available from: https://scholarbank.nus.edu.sg/handle/10635/168230.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

SHUJUAN H. MEMBRANE FOULING CONTROL IN INDUSTRIAL WASTEWATER TREATMENT. [Thesis]. National University of Singapore; 2020. Available from: https://scholarbank.nus.edu.sg/handle/10635/168230

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

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Open Access Theses and Dissertations