subject:(pyoderma)

Texas A&M University

1. Gold, Randi. Carriage of Virulence Factors and Antimicrobial Resistance Genes in Staphylococcus pseudintermedius from Dogs.

Degree: PhD, Veterinary Microbiology, 2015, Texas A&M University

URL: http://hdl.handle.net/1969.1/155019

► Staphylococcus pseudintermedius is the most common microorganism isolated from canine pyoderma and opportunistic infections. Prevalence of methicillin-resistant S. pseudintermedius (MRSP) has increased and multi-drug resistance… (more)

▼ Staphylococcus pseudintermedius is the most common microorganism isolated from canine pyoderma and opportunistic infections. Prevalence of methicillin-resistant S. pseudintermedius (MRSP) has increased and multi-drug resistance has become common. A total of 734 S. pseudintermedius isolates collected from dogs presented to the Veterinary Medical Teaching Hospital from 2007 to 2012 were studied. Isolates were analyzed for antimicrobial resistance and virulence gene carriage. With the emergence of methicillin resistance, veterinarians have begun to use antimicrobials such as amikacin, to treat life-threatening MRSP infections. The most widespread mechanism of amikacin resistance is drug inactivation by aminoglycoside modifying enzymes (AMEs). The most prevalent gene detected here was aph(3′)- IIIa found in 75% (24/32) of isolates followed by aac(6′)/aph(2′′) and ant(4′)-Ia in 12% (4/32) and 3% (1/32), respectively. There was a significant association between amikacin and methicillin resistance. Since AMEs can be transferred from one bacteria to another, amikacin resistance may represent a new nosocomial and zoonotic threat. Clindamycin is an alternative to β-lactam antimicrobial therapy for canine pyoderma. Inducible and constitutive resistance to clindamycin can occur. Approximately forty erm genes encoding methylases involved in clindamycin resistance have been reported, with ermB most commonly found among S. pseudintermedius. We found eight of 608 isolates tested, positive for inducible clindamycin resistance by D-test and PCR detection of ermB. A vaccine against staphylococcal pyoderma would reduce the reliance on antimicrobial drugs. Staphylococcal cell-wall associated proteins (CWAPs) involved in colonization of the host are attractive potential vaccine targets. Eighteen CWAPs encoded by sps genes have been described in S. pseudintermedius; however, four vary in occurrence. Isolates were analyzed by polymerase chain reaction (PCR) for the presence of mecA, SCCmec type I-VI, and spsF, spsO, spsP, and spsQ. There was a significant association between methicillin resistance and carriage of spsP and spsQ. spsP and spsQ may be viable vaccine targets. Advisors/Committee Members: Lawhon, Sara (advisor), Cohen, Noah (committee member), Rodrigues, Aline (committee member), Fajt, Virginia (committee member), Patterson, Adam (committee member).

Subjects/Keywords: staphylococcus pseudintermedius; pyoderma; methicillin-resistant; amikacin resistance; inducible clindamycin resistance

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APA (6^th Edition):

Gold, R. (2015). Carriage of Virulence Factors and Antimicrobial Resistance Genes in Staphylococcus pseudintermedius from Dogs. (Doctoral Dissertation). Texas A&M University. Retrieved from http://hdl.handle.net/1969.1/155019

Chicago Manual of Style (16^th Edition):

Gold, Randi. “Carriage of Virulence Factors and Antimicrobial Resistance Genes in Staphylococcus pseudintermedius from Dogs.” 2015. Doctoral Dissertation, Texas A&M University. Accessed January 18, 2021. http://hdl.handle.net/1969.1/155019.

MLA Handbook (7^th Edition):

Gold, Randi. “Carriage of Virulence Factors and Antimicrobial Resistance Genes in Staphylococcus pseudintermedius from Dogs.” 2015. Web. 18 Jan 2021.

Vancouver:

Gold R. Carriage of Virulence Factors and Antimicrobial Resistance Genes in Staphylococcus pseudintermedius from Dogs. [Internet] [Doctoral dissertation]. Texas A&M University; 2015. [cited 2021 Jan 18]. Available from: http://hdl.handle.net/1969.1/155019.

Council of Science Editors:

Gold R. Carriage of Virulence Factors and Antimicrobial Resistance Genes in Staphylococcus pseudintermedius from Dogs. [Doctoral Dissertation]. Texas A&M University; 2015. Available from: http://hdl.handle.net/1969.1/155019

2. Ansar Kamran C. Pyoderma in dogs;.

Degree: Animal reproduction, 2006, Karnataka Veterinary, Animal and Fisheries Sciences University

URL: http://shodhganga.inflibnet.ac.in/handle/10603/6947

None

Summary, References and Appendix included

Advisors/Committee Members: Yathiraj S.

Subjects/Keywords: Veterinary Sciences; Animal reproduction; Pyoderma; Dogs

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APA (6^th Edition):

C, A. K. (2006). Pyoderma in dogs;. (Thesis). Karnataka Veterinary, Animal and Fisheries Sciences University. Retrieved from http://shodhganga.inflibnet.ac.in/handle/10603/6947

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

C, Ansar Kamran. “Pyoderma in dogs;.” 2006. Thesis, Karnataka Veterinary, Animal and Fisheries Sciences University. Accessed January 18, 2021. http://shodhganga.inflibnet.ac.in/handle/10603/6947.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

C, Ansar Kamran. “Pyoderma in dogs;.” 2006. Web. 18 Jan 2021.

Vancouver:

C AK. Pyoderma in dogs;. [Internet] [Thesis]. Karnataka Veterinary, Animal and Fisheries Sciences University; 2006. [cited 2021 Jan 18]. Available from: http://shodhganga.inflibnet.ac.in/handle/10603/6947.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

C AK. Pyoderma in dogs;. [Thesis]. Karnataka Veterinary, Animal and Fisheries Sciences University; 2006. Available from: http://shodhganga.inflibnet.ac.in/handle/10603/6947

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Cape Town

3. Lawrence, Pat. Pyoderma gangrenosum : the Groote Schuur Hospital experience, 1970-1990.

Degree: Image, Division of Dermatology, 1991, University of Cape Town

URL: http://hdl.handle.net/11427/25568

Subjects/Keywords: Pyoderma - Groote Schuur Hospital, 1970-1990; Pyoderma - diagnosis - Groote Schuur Hospital, 1970-1990; Pyoderma - drug therapy - Groote Schuur Hospital, 1970-1990

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APA (6^th Edition):

Lawrence, P. (1991). Pyoderma gangrenosum : the Groote Schuur Hospital experience, 1970-1990. (Thesis). University of Cape Town. Retrieved from http://hdl.handle.net/11427/25568

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Lawrence, Pat. “Pyoderma gangrenosum : the Groote Schuur Hospital experience, 1970-1990.” 1991. Thesis, University of Cape Town. Accessed January 18, 2021. http://hdl.handle.net/11427/25568.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Lawrence, Pat. “Pyoderma gangrenosum : the Groote Schuur Hospital experience, 1970-1990.” 1991. Web. 18 Jan 2021.

Vancouver:

Lawrence P. Pyoderma gangrenosum : the Groote Schuur Hospital experience, 1970-1990. [Internet] [Thesis]. University of Cape Town; 1991. [cited 2021 Jan 18]. Available from: http://hdl.handle.net/11427/25568.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Lawrence P. Pyoderma gangrenosum : the Groote Schuur Hospital experience, 1970-1990. [Thesis]. University of Cape Town; 1991. Available from: http://hdl.handle.net/11427/25568

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

4. Mercer, Heather Milliken. The Distribution of Single Nucleotide Polymorphisms in Pyoderma Gangrenosum: Biomarker Discovery.

Degree: MS, College of Arts and Sciences / Department of Biological Sciences, 2013, Kent State University

URL: http://rave.ohiolink.edu/etdc/view?acc_num=kent1383769612

► Pyoderma gangrenosum (PG) is a rare, inflammatory disease marked by reactive, neutrophilic dermatosis. While PG is non-infectious, it is painful, only diagnosed by exclusion of… (more)

▼ Pyoderma gangrenosum (PG) is a rare, inflammatory disease marked by reactive, neutrophilic dermatosis. While PG is non-infectious, it is painful, only diagnosed by exclusion of other disorders (Bhat, 2012), and challenging to treat. Thus, there exists a need for discovery of biomarkers that can aid in faster and more reliable diagnosis. Whole-genome genotyping platforms- such as Affymetrix SNP 6.0 chip - offer rapid and comprehensive ways to survey patients' genomes to identify shared genetic polymorphisms as part of the biomarker discovery.In this work we test the hypothesis that PG is caused by genetic variants within the genome that (1) inhibit neutrophil clearance and immune/inflammatory pathways and (2) that a combination of Single Nucleotide Polymorphisms (SNPs) across multiple loci in this pathway may contribute to this dysfunction. We examined a list of 64,997 SNPs found in 9,831 genes that were shared across six PG patients. These genes were examined to determine whether an over-enrichment of immune- and/or inflammation and/or apoptosis variants exists across patients using Gene Ontology tools. Gene Ontology tools provided insight into the functional classifications of these genes and possible connections to PG etiology. The genes found in common between the PG data set and those known to be active in apoptotic and immunity/inflammatory pathways were further analyzed for the potential effects of genetic variation. Those genetic variants located within exons and 5' and 3' untranslated regions in addition to those that cause missense or nonsense were viewed as the most likely to be related to PG as mutations in those regions are most likely to alter protein products. In addition to the aforementioned genetic regions, those SNPS located in non-coding genic regions (potentially regulatory) as well as those from the inter-genic regions were analyzed. The latter category, while currently lacking known functional annotations, may provide insight into the genome function as putative regulatory regions and/or non-coding RNA as suggested by recent endeavors such as the ENCODE project. Analyses resulted in the discovery of an over-enrichment of SNPs within genes functioning within both apoptotic and immune/inflammatory pathways within the PG Data Set in addition to SNPS located in genes that may be integral to the development of PG. Advisors/Committee Members: Piontkivska, Helen (Advisor).

Subjects/Keywords: Biology; Bioinformatics; Genetics; Pyoderma Gangrenosum; SNP; Single Nucleotide Polymorphism; Genetic Variant; Biomarkers

…Chapter 1: Pyoderma Gangrenosum, Inflammation, and Apoptosis Table 1: List of diseases which are… …of Classic, Ulcerative Pyoderma Gangrenosum (PG). Diagnosis requires both major… …genes ��p. 101 xii LIST OF ABBREVIATIONS PG ��.Pyoderma Gangrenosum SNPs ��Single Nucleotide… …pyoderma gangrenosum, and acne IBD ��Irritable Bowel Syndrome RA ��Rheumatoid Arthritis UC… …POLYMORPHISMS IN PYODERMA GANGRENOSUM: BIOMARKER DISCOVERY I. To be completed by the student: I…

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Mercer, H. M. (2013). The Distribution of Single Nucleotide Polymorphisms in Pyoderma Gangrenosum: Biomarker Discovery. (Masters Thesis). Kent State University. Retrieved from http://rave.ohiolink.edu/etdc/view?acc_num=kent1383769612

Chicago Manual of Style (16^th Edition):

Mercer, Heather Milliken. “The Distribution of Single Nucleotide Polymorphisms in Pyoderma Gangrenosum: Biomarker Discovery.” 2013. Masters Thesis, Kent State University. Accessed January 18, 2021. http://rave.ohiolink.edu/etdc/view?acc_num=kent1383769612.

MLA Handbook (7^th Edition):

Mercer, Heather Milliken. “The Distribution of Single Nucleotide Polymorphisms in Pyoderma Gangrenosum: Biomarker Discovery.” 2013. Web. 18 Jan 2021.

Vancouver:

Mercer HM. The Distribution of Single Nucleotide Polymorphisms in Pyoderma Gangrenosum: Biomarker Discovery. [Internet] [Masters thesis]. Kent State University; 2013. [cited 2021 Jan 18]. Available from: http://rave.ohiolink.edu/etdc/view?acc_num=kent1383769612.

Council of Science Editors:

Mercer HM. The Distribution of Single Nucleotide Polymorphisms in Pyoderma Gangrenosum: Biomarker Discovery. [Masters Thesis]. Kent State University; 2013. Available from: http://rave.ohiolink.edu/etdc/view?acc_num=kent1383769612

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