subject:(proteomic)

NSYSU

1. Kuo, Tzu-Lei. Proteomic Analysis of Membrane Fraction of Rhabdomyosarcoma Cells in Response to Early Enterovirus 71 Infection.

Degree: Master, Institute of Biomedical Sciences, 2009, NSYSU

URL: http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0728109-142101

► Enterovirus 71 (EV71) infection is one of epidemic disease in children commonly in Taiwan. The clinical manifestation of EV71 infection may include acute respiratory disease,… (more)

Subjects/Keywords: EV71; membrane proteomic

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Kuo, T. (2009). Proteomic Analysis of Membrane Fraction of Rhabdomyosarcoma Cells in Response to Early Enterovirus 71 Infection. (Thesis). NSYSU. Retrieved from http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0728109-142101

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Kuo, Tzu-Lei. “Proteomic Analysis of Membrane Fraction of Rhabdomyosarcoma Cells in Response to Early Enterovirus 71 Infection.” 2009. Thesis, NSYSU. Accessed January 23, 2021. http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0728109-142101.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Kuo, Tzu-Lei. “Proteomic Analysis of Membrane Fraction of Rhabdomyosarcoma Cells in Response to Early Enterovirus 71 Infection.” 2009. Web. 23 Jan 2021.

Vancouver:

Kuo T. Proteomic Analysis of Membrane Fraction of Rhabdomyosarcoma Cells in Response to Early Enterovirus 71 Infection. [Internet] [Thesis]. NSYSU; 2009. [cited 2021 Jan 23]. Available from: http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0728109-142101.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Kuo T. Proteomic Analysis of Membrane Fraction of Rhabdomyosarcoma Cells in Response to Early Enterovirus 71 Infection. [Thesis]. NSYSU; 2009. Available from: http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0728109-142101

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

2. Télot, Lorène. Pour une meilleure compréhension de la physiopathologie de l'Ataxie de Friedreich : apport de protéomique quantitative pour la caractérisation des mécanismes moléculaires altérés : For a better understanding of the physiopathology of Friedreich’ataxia : the contribution of quantitative proteomics for the characterization of altered molecular mechanisms.

Degree: Docteur es, Physiologie et biologie des organismes - populations - interactions. Biologie moléculaire, 2017, Sorbonne Paris Cité

URL: http://www.theses.fr/2017USPCC301

►

L’ataxie de Friedreich (AF) est une maladie neurodégénérative à transmission autosomique récessive. Cette pathologie se caractérise par une dégénérescence spinocérébelleuse, une cardiomyopathie hypertrophique qui est… (more)

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L’ataxie de Friedreich (AF) est une maladie neurodégénérative à transmission autosomique récessive. Cette pathologie se caractérise par une dégénérescence spinocérébelleuse, une cardiomyopathie hypertrophique qui est la cause majeure du décès des patients, et un risque accru de diabète. La mutation majoritaire causant l’AF est une hyper-expansion de triplet GAA dans le premier intron du gène FXN codant la frataxine, une protéine mitochondriale ubiquitaire codée par le génome nucléaire. Ces hyper-expansions instables conduisent à une inhibition de la transcription du gène FXN et donc à une baisse d’expression de la frataxine. Aucun traitement curatif n’est disponible à l’heure actuelle pour cette maladie. Seule une meilleure compréhension de la physiopathologie de l’AF permettra d’envisager le développement de stratégies thérapeutiques efficaces. Plusieurs travaux montrent que la frataxine intervient dans la biosynthèse des centres Fe-S, mais son rôle exact dans cette voie, et sa possible contribution dans d’autres processus biochimiques, doivent encore être élucidés. Par une approche de protéomique quantitative utilisée pour la première fois sur des lignées lymphocytaires issues d’un patient AF et d’un individu non atteint, nous avons pu établir le profil d’expression des protéines associées à un déficit en frataxine. Ces nouvelles données confirment les processus altérés décrits pour l’AF, et ont permis la mise en exergue de nouveaux mécanismes mitochondriaux impactés, comme l’altération de la voie d’importation via CHCHD4. La mitochondrie interagissant avec le réticulum endoplasmique (RE), nous avons analysé et comparé l’impact d’un stress induit par la thapsigargine ciblant le RE sur le profil d’expression des protéines des lymphocytes B AF et contrôles. Ces analyses montrent que le déficit en frataxine rend les mitochondries des cellules de patients AF plus sensibles à un stress du RE, nécessitant la mise en place de réponses adaptatives spécifiques. L’approfondissement des mécanismes altérés associés au déficit en frataxine, avec et sans stress exogène, permettront d’une part, de mieux comprendre la pathogenèse de l’AF et d’autre part, de proposer des stratégies thérapeutiques adaptées.

Friedreich’s ataxia (FRDA) represents the most frequent type of autosomal-recessively inherited ataxia associated with a cardiomyopathy, which is the main cause of the death, and a risk of diabetes. FRDA is caused by mutations in the FXN gene, encoding mitochondrial frataxin, arising from an unstable hyperexpansion of GAA triplet repeats in the first intron of the gene. This hyperexpansion leads to FXN gene silencing and a quantitative decreased expression of frataxin. However despite many efforts to overcome any of these abnormalities, there is currently no efficient treatment to cure or even stop the progression of this disease, mostly because many aspects of the pathological consequences of frataxin depletion are still not fully understood. The precise role of frataxin is still under debate. A key function of frataxin in…

Advisors/Committee Members: Serre, Valérie (thesis director).

Subjects/Keywords: Protéomique quantitative; Proteomic quantitative approach

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Télot, L. (2017). Pour une meilleure compréhension de la physiopathologie de l'Ataxie de Friedreich : apport de protéomique quantitative pour la caractérisation des mécanismes moléculaires altérés : For a better understanding of the physiopathology of Friedreich’ataxia : the contribution of quantitative proteomics for the characterization of altered molecular mechanisms. (Doctoral Dissertation). Sorbonne Paris Cité. Retrieved from http://www.theses.fr/2017USPCC301

Chicago Manual of Style (16^th Edition):

Télot, Lorène. “Pour une meilleure compréhension de la physiopathologie de l'Ataxie de Friedreich : apport de protéomique quantitative pour la caractérisation des mécanismes moléculaires altérés : For a better understanding of the physiopathology of Friedreich’ataxia : the contribution of quantitative proteomics for the characterization of altered molecular mechanisms.” 2017. Doctoral Dissertation, Sorbonne Paris Cité. Accessed January 23, 2021. http://www.theses.fr/2017USPCC301.

MLA Handbook (7^th Edition):

Télot, Lorène. “Pour une meilleure compréhension de la physiopathologie de l'Ataxie de Friedreich : apport de protéomique quantitative pour la caractérisation des mécanismes moléculaires altérés : For a better understanding of the physiopathology of Friedreich’ataxia : the contribution of quantitative proteomics for the characterization of altered molecular mechanisms.” 2017. Web. 23 Jan 2021.

Vancouver:

Télot L. Pour une meilleure compréhension de la physiopathologie de l'Ataxie de Friedreich : apport de protéomique quantitative pour la caractérisation des mécanismes moléculaires altérés : For a better understanding of the physiopathology of Friedreich’ataxia : the contribution of quantitative proteomics for the characterization of altered molecular mechanisms. [Internet] [Doctoral dissertation]. Sorbonne Paris Cité; 2017. [cited 2021 Jan 23]. Available from: http://www.theses.fr/2017USPCC301.

Council of Science Editors:

Télot L. Pour une meilleure compréhension de la physiopathologie de l'Ataxie de Friedreich : apport de protéomique quantitative pour la caractérisation des mécanismes moléculaires altérés : For a better understanding of the physiopathology of Friedreich’ataxia : the contribution of quantitative proteomics for the characterization of altered molecular mechanisms. [Doctoral Dissertation]. Sorbonne Paris Cité; 2017. Available from: http://www.theses.fr/2017USPCC301

University College Cork

3. Jaafar, Siti NurTahirah. Proteomic approaches to environmental stress in mussel Mytilus edulis due to emerging classes of anthropogenic pollutants.

Degree: 2015, University College Cork

URL: http://hdl.handle.net/10468/2102

► Anthropogenic pollutant chemicals pose a major threat to aquatic organisms. There is a need for more research on emerging categories of environmental chemicals such as… (more)

▼ Anthropogenic pollutant chemicals pose a major threat to aquatic organisms. There is a need for more research on emerging categories of environmental chemicals such as nanomaterials, endocrine disruptors and pharmaceuticals. Proteomics offers options and advantages for early warning of alterations in environmental quality by detecting sub-lethal changes in sentinel species such as the mussel, Mytilus edulis. This thesis aimed to compare the potential of traditional biomarkers (such as enzyme activity measurement) and newer redox proteomic approaches. Environmental proteomics, especially a redox proteomics toolbox, may be a novel way to study pollutant effects on organisms which can also yield information on risks to human health. In particular, it can probe subtle biochemical changes at sub-lethal concentrations and thus offer novel insights to toxicity mechanisms. In the first instance, the present research involved a field-study in three stations in Cork Harbour, Ireland (Haulbowline, Ringaskiddy and Douglas) compared to an outharbour control site in Bantry Bay, Ireland. Then, further research was carried out to detect effects of anthropogenic pollution on selected chemicals. Diclofenac is an example of veterinary and human pharmaceuticals, an emerging category of chemical pollutants, with potential to cause serious toxicity to non-target organisms. A second chemical used for this study was copper which is a key source of contamination in marine ecosystems. Thirdly, bisphenol A is a major anthropogenic chemical mainly used in polycarbonate plastics manufacturing that is widespread in the environment. It is also suspected to be an endocrine disruptor. Effects on the gill, the principal feeding organ of mussels, were investigated in particular. Effects on digestive gland were also investigated to compare different outcomes from each tissue. Across the three anthropogenic chemicals studied (diclofenac, copper and bisphenol A), only diclofenac exposure did not show any significant difference towards glutathione transferase (GST) responses. Meanwhile, copper and bisphenol A significantly increased GST in gill. Glutathione reductase (GR) enzyme analysis revealed that all three chemicals have significant responses in gill. Catalase activity showed significant differences in digestive gland exposed to diclofenac and gills exposed to bisphenol A. This study focused then on application of redox proteomics; the study of the oxidative modification of proteins, to M. edulis. Thiol proteins were labelled with 5-iodoacetamidofluorescein prior to one-dimensional and two-dimensional electrophoresis. This clearly revealed some similarities on a portion of the redox proteome across chemical exposures indicating where toxicity mechanism may be common and where effects are unique to a single treatment. This thesis documents that proteomics is a robust tool to provide valuable insights into possible mechanisms of toxicity of anthropogenic contaminants in M. edulis. It is concluded that future research should focus on gill tissue, on… Advisors/Committee Members: Sheehan, David.

Subjects/Keywords: Proteomic; Mytilus edulis; Anthropogenic pollutants

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Jaafar, S. N. (2015). Proteomic approaches to environmental stress in mussel Mytilus edulis due to emerging classes of anthropogenic pollutants. (Thesis). University College Cork. Retrieved from http://hdl.handle.net/10468/2102

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Jaafar, Siti NurTahirah. “Proteomic approaches to environmental stress in mussel Mytilus edulis due to emerging classes of anthropogenic pollutants.” 2015. Thesis, University College Cork. Accessed January 23, 2021. http://hdl.handle.net/10468/2102.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Jaafar, Siti NurTahirah. “Proteomic approaches to environmental stress in mussel Mytilus edulis due to emerging classes of anthropogenic pollutants.” 2015. Web. 23 Jan 2021.

Vancouver:

Jaafar SN. Proteomic approaches to environmental stress in mussel Mytilus edulis due to emerging classes of anthropogenic pollutants. [Internet] [Thesis]. University College Cork; 2015. [cited 2021 Jan 23]. Available from: http://hdl.handle.net/10468/2102.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Jaafar SN. Proteomic approaches to environmental stress in mussel Mytilus edulis due to emerging classes of anthropogenic pollutants. [Thesis]. University College Cork; 2015. Available from: http://hdl.handle.net/10468/2102

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

4. Owens, Rebecca A. A Global and Targeted Proteomic Investigation of Aspergillus fumigatus.

Degree: 2012, RIAN

URL: http://eprints.maynoothuniversity.ie/6696/

► Aspergillus fumigatus is an opportunistic pathogen that can cause invasive disease in immunocompromised individuals and, less frequently, in immunocompetent hosts. Proteomic investigation of A. fumigatus… (more)

▼ Aspergillus fumigatus is an opportunistic pathogen that can cause invasive disease in immunocompromised individuals and, less frequently, in immunocompetent hosts. Proteomic investigation of A. fumigatus has the potential to enable global analysis of protein expression, identify potential targets for vaccine or diagnostic tool development, and characterise system-wide responses to external stimuli. Implementation of a large-scale proteomic strategy lead to the identification of non-redundant proteins from mycelia (n = 390) and culture supernatants (n = 42) of A. fumigatus. Utilisation of MS-based proteomics facilitated the identification of proteins typically under-represented in 2D-PAGE proteome maps, including proteins with multiple transmembrane regions, hydrophobic proteins and proteins with extremes of molecular mass and pI. Pre-fractionation of complex protein samples, by gel-filtration or gold nanoparticle pre-incubation, demonstrated potential for reduction of sample complexity. Indirect identification of secondary metabolite cluster expression was achieved using a global MS-based proteomic approach, with proteins (n = 20) from LaeA-regulated clusters detected. Targeted immunoproteomics resulted in the identification of antigenic proteins (n = 25) from A. fumigatus, reactive with sera from healthy individuals, and characterisation of these proteins may shed light on the pathobiology of A. fumigatus. Mechanisms involved in the interaction of A. fumigatus with gliotoxin were also examined, using phenotypic analysis, comparative proteomics and metabolomics. Gliotoxin was observed to relieve H2O2-induced stress, in a dose-dependent manner (0 - 10 μg/ml) and this correlated with a significant increase in expression of the gliotoxin oxidoreductase GliT (p < 0.05). This indicates a role for gliotoxin, and potentially GliT, in relief of oxidative stress in A. fumigatus. Correspondingly, proteins associated with response to stress were observed to significantly decrease in expression in the co-addition condition, relative to H2O2 alone (p < 0.05). Comparative proteomic profiling of the gliotoxin-sensitive mutant, A. fumigatus ΔgliK, revealed perturbation of translation, the methyl cycle and the endoplasmic reticulum in response to gliotoxin. This informs on the mechanisms involved in gliotoxin-mediated toxicity and may apply to other gliotoxin-sensitive species. Loss of gliotoxin production in A. fumigatus ΔgliK correlated with significant elevation in intracellular ergothioneine levels (p < 0.001). This study describes the first identification of ergothioneine in A. fumigatus and represents a target for future redox investigations.

Subjects/Keywords: Proteomic Investigation; Aspergillus fumigatus

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Owens, R. A. (2012). A Global and Targeted Proteomic Investigation of Aspergillus fumigatus. (Thesis). RIAN. Retrieved from http://eprints.maynoothuniversity.ie/6696/

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Owens, Rebecca A. “A Global and Targeted Proteomic Investigation of Aspergillus fumigatus.” 2012. Thesis, RIAN. Accessed January 23, 2021. http://eprints.maynoothuniversity.ie/6696/.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Owens, Rebecca A. “A Global and Targeted Proteomic Investigation of Aspergillus fumigatus.” 2012. Web. 23 Jan 2021.

Vancouver:

Owens RA. A Global and Targeted Proteomic Investigation of Aspergillus fumigatus. [Internet] [Thesis]. RIAN; 2012. [cited 2021 Jan 23]. Available from: http://eprints.maynoothuniversity.ie/6696/.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Owens RA. A Global and Targeted Proteomic Investigation of Aspergillus fumigatus. [Thesis]. RIAN; 2012. Available from: http://eprints.maynoothuniversity.ie/6696/

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

5. Owens, Rebecca A. A Global and Targeted Proteomic Investigation of Aspergillus fumigatus.

Degree: 2012, RIAN

URL: http://mural.maynoothuniversity.ie/6696/

► Aspergillus fumigatus is an opportunistic pathogen that can cause invasive disease in immunocompromised individuals and, less frequently, in immunocompetent hosts. Proteomic investigation of A. fumigatus… (more)

▼ Aspergillus fumigatus is an opportunistic pathogen that can cause invasive disease in immunocompromised individuals and, less frequently, in immunocompetent hosts. Proteomic investigation of A. fumigatus has the potential to enable global analysis of protein expression, identify potential targets for vaccine or diagnostic tool development, and characterise system-wide responses to external stimuli. Implementation of a large-scale proteomic strategy lead to the identification of non-redundant proteins from mycelia (n = 390) and culture supernatants (n = 42) of A. fumigatus. Utilisation of MS-based proteomics facilitated the identification of proteins typically under-represented in 2D-PAGE proteome maps, including proteins with multiple transmembrane regions, hydrophobic proteins and proteins with extremes of molecular mass and pI. Pre-fractionation of complex protein samples, by gel-filtration or gold nanoparticle pre-incubation, demonstrated potential for reduction of sample complexity. Indirect identification of secondary metabolite cluster expression was achieved using a global MS-based proteomic approach, with proteins (n = 20) from LaeA-regulated clusters detected. Targeted immunoproteomics resulted in the identification of antigenic proteins (n = 25) from A. fumigatus, reactive with sera from healthy individuals, and characterisation of these proteins may shed light on the pathobiology of A. fumigatus. Mechanisms involved in the interaction of A. fumigatus with gliotoxin were also examined, using phenotypic analysis, comparative proteomics and metabolomics. Gliotoxin was observed to relieve H2O2-induced stress, in a dose-dependent manner (0 - 10 μg/ml) and this correlated with a significant increase in expression of the gliotoxin oxidoreductase GliT (p < 0.05). This indicates a role for gliotoxin, and potentially GliT, in relief of oxidative stress in A. fumigatus. Correspondingly, proteins associated with response to stress were observed to significantly decrease in expression in the co-addition condition, relative to H2O2 alone (p < 0.05). Comparative proteomic profiling of the gliotoxin-sensitive mutant, A. fumigatus ΔgliK, revealed perturbation of translation, the methyl cycle and the endoplasmic reticulum in response to gliotoxin. This informs on the mechanisms involved in gliotoxin-mediated toxicity and may apply to other gliotoxin-sensitive species. Loss of gliotoxin production in A. fumigatus ΔgliK correlated with significant elevation in intracellular ergothioneine levels (p < 0.001). This study describes the first identification of ergothioneine in A. fumigatus and represents a target for future redox investigations.

Subjects/Keywords: Proteomic Investigation; Aspergillus fumigatus

Record Details Similar Records Cite « Share

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Owens, R. A. (2012). A Global and Targeted Proteomic Investigation of Aspergillus fumigatus. (Thesis). RIAN. Retrieved from http://mural.maynoothuniversity.ie/6696/

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Owens, Rebecca A. “A Global and Targeted Proteomic Investigation of Aspergillus fumigatus.” 2012. Thesis, RIAN. Accessed January 23, 2021. http://mural.maynoothuniversity.ie/6696/.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Owens, Rebecca A. “A Global and Targeted Proteomic Investigation of Aspergillus fumigatus.” 2012. Web. 23 Jan 2021.

Vancouver:

Owens RA. A Global and Targeted Proteomic Investigation of Aspergillus fumigatus. [Internet] [Thesis]. RIAN; 2012. [cited 2021 Jan 23]. Available from: http://mural.maynoothuniversity.ie/6696/.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Owens RA. A Global and Targeted Proteomic Investigation of Aspergillus fumigatus. [Thesis]. RIAN; 2012. Available from: http://mural.maynoothuniversity.ie/6696/

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

6. Wheatley, Matthew D. Abiotic-Stress Application, Global Berry Proteome Profiling, and Cold-Stress Tolerance in Transformed Winegrape (Vitis vinifera L.).

Degree: 2011, University of Nevada – Reno

URL: http://hdl.handle.net/11714/3792

► Wine grape (Vitis vinifera L.) was domesticated more than 7,000 years ago and continues to provide one of the most important fruit crops worldwide. V.… (more)

▼ Wine grape (Vitis vinifera L.) was domesticated more than 7,000 years ago and continues to provide one of the most important fruit crops worldwide. V. vinifera cultivars are moderately sensitive to salt and temperature stresses, whereas moderate water deficit stress results in minimal loss in berry yields, increased berry quality, and higher rates of winter survival of vines. The molecular mechanisms controlling both the desirable and undesirable effects of these stresses are poorly understood. To lay the foundation for our current understanding into the molecular mechanisms that underlie these environmental stress responses, a survey of the state-of-the-art literature was performed (Chapter I). For elicitation of a pronounced change in abundance of transcripts in response to abiotic-stresses a recirculating drip hydroponic growth system was built using expanded clay ball media to eliminate the buffering effect of soil. This system effectively ameliorated stress-related symptoms of hypoxia exhibited by vines grown using a previously described media-free hydroponic method (Chapter II). Our understanding of the complex nature of the V. vinifera berry proteome is extremely limited. Using a phenol-based protein extraction protocol, large two-dimensional polyacrylamide gels, and separation of proteins across two overlapping isoelectic focusing (IEF) ranges spanning pI 4-11, a total of 802 proteins were identified from whole grape berries across four distinct phases (phases I-IV) of berry development including immature green berries, lag phase, veráison, and mature berries spanning modified Eichhorn and Lorennz (E-L) system stages 29-38. Abundance profiles were generated by two-dimensional hierarchical clustering for 652 proteins whose abundance profiles could be confirmed across all four phases of berry development resulting in 21 discrete clusters. Profiles were also compared between berries collected from well watered and water deficit treated vines. Fifty one proteins showed significant differences in relative abundance in response to water deficit stress at one or more developmental phases. Comparison of the current results with previously published studies indicated that 606 of the proteins identified were previously undescribed making the current study the most comprehensive ever undertaken to date. Novel insights included the discovery that several pathogenesis-related (PR) proteins appear during early berry development, seed-storage proteins reach maximal abundance during phase III, and programmed cell death (PCD) proteins and associated salvage enzymes peak during phase IV. Water deficit stress resulted in the decreased abundance of proteins associated with mitochondrial energy production, but in the increased abundance of proteins associated with pathogenesis and heat shock responses as well as photosynthetic-, ABA-induced osmotic- and nutrient deficit-stress responses and phenylpropanoid biosynthetic enzymes (Chapter III). To investigate the effect of constitutive expression of a Vitis vinifera CBF… Advisors/Committee Members: Cushman, John C. (advisor), Blomquist, Gary (committee member), Schegg, Kathleen (committee member), Shintani, David (committee member), Berninsone, Patricia (committee member).

Subjects/Keywords: DIGE; grape; proteomic; transgenic

Record Details Similar Records Cite « Share

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Wheatley, M. D. (2011). Abiotic-Stress Application, Global Berry Proteome Profiling, and Cold-Stress Tolerance in Transformed Winegrape (Vitis vinifera L.). (Thesis). University of Nevada – Reno. Retrieved from http://hdl.handle.net/11714/3792

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Wheatley, Matthew D. “Abiotic-Stress Application, Global Berry Proteome Profiling, and Cold-Stress Tolerance in Transformed Winegrape (Vitis vinifera L.).” 2011. Thesis, University of Nevada – Reno. Accessed January 23, 2021. http://hdl.handle.net/11714/3792.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Wheatley, Matthew D. “Abiotic-Stress Application, Global Berry Proteome Profiling, and Cold-Stress Tolerance in Transformed Winegrape (Vitis vinifera L.).” 2011. Web. 23 Jan 2021.

Vancouver:

Wheatley MD. Abiotic-Stress Application, Global Berry Proteome Profiling, and Cold-Stress Tolerance in Transformed Winegrape (Vitis vinifera L.). [Internet] [Thesis]. University of Nevada – Reno; 2011. [cited 2021 Jan 23]. Available from: http://hdl.handle.net/11714/3792.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Wheatley MD. Abiotic-Stress Application, Global Berry Proteome Profiling, and Cold-Stress Tolerance in Transformed Winegrape (Vitis vinifera L.). [Thesis]. University of Nevada – Reno; 2011. Available from: http://hdl.handle.net/11714/3792

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

NSYSU

7. Hou, You-syuan. Using proteomic approach to discover novel biomarkers involved in chemoresistance to B-RAF inhibitor in thyroid cancer.

Degree: Master, Institute of Biomedical Sciences, 2014, NSYSU

URL: http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0631114-003207

► Thyroid carcinoma (TC) is the most common endocrine malignancy and is one of the most rapidly increasing human cancers worldwide. Anaplastic thyroid carcinoma (ATC) is… (more)

Subjects/Keywords: thyroid cancer; BRAF inhibitor; proteomic; apoptosis; resistance

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Hou, Y. (2014). Using proteomic approach to discover novel biomarkers involved in chemoresistance to B-RAF inhibitor in thyroid cancer. (Thesis). NSYSU. Retrieved from http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0631114-003207

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Hou, You-syuan. “Using proteomic approach to discover novel biomarkers involved in chemoresistance to B-RAF inhibitor in thyroid cancer.” 2014. Thesis, NSYSU. Accessed January 23, 2021. http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0631114-003207.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Hou, You-syuan. “Using proteomic approach to discover novel biomarkers involved in chemoresistance to B-RAF inhibitor in thyroid cancer.” 2014. Web. 23 Jan 2021.

Vancouver:

Hou Y. Using proteomic approach to discover novel biomarkers involved in chemoresistance to B-RAF inhibitor in thyroid cancer. [Internet] [Thesis]. NSYSU; 2014. [cited 2021 Jan 23]. Available from: http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0631114-003207.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Hou Y. Using proteomic approach to discover novel biomarkers involved in chemoresistance to B-RAF inhibitor in thyroid cancer. [Thesis]. NSYSU; 2014. Available from: http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0631114-003207

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Mississippi State University

8. Payne, Angela Inez. RESPONSE OF LISTERIA MONOCYTOGENES TO BILE SALTS.

Degree: MS, Biological Sciences, 2012, Mississippi State University

URL: http://sun.library.msstate.edu/ETD-db/theses/available/etd-04022012-085153/ ;

► <i>Listeria monocytogenes</i> is a food-borne pathogen responsible for the disease listeriosis. The infectious process depends upon survival in high bile salt conditions encountered throughout… (more)

Subjects/Keywords: proteomic analysis; anaerobic; bile salts; Listeria monocytogenes

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APA (6^th Edition):

Payne, A. I. (2012). RESPONSE OF LISTERIA MONOCYTOGENES TO BILE SALTS. (Masters Thesis). Mississippi State University. Retrieved from http://sun.library.msstate.edu/ETD-db/theses/available/etd-04022012-085153/ ;

Chicago Manual of Style (16^th Edition):

Payne, Angela Inez. “RESPONSE OF LISTERIA MONOCYTOGENES TO BILE SALTS.” 2012. Masters Thesis, Mississippi State University. Accessed January 23, 2021. http://sun.library.msstate.edu/ETD-db/theses/available/etd-04022012-085153/ ;.

MLA Handbook (7^th Edition):

Payne, Angela Inez. “RESPONSE OF LISTERIA MONOCYTOGENES TO BILE SALTS.” 2012. Web. 23 Jan 2021.

Vancouver:

Payne AI. RESPONSE OF LISTERIA MONOCYTOGENES TO BILE SALTS. [Internet] [Masters thesis]. Mississippi State University; 2012. [cited 2021 Jan 23]. Available from: http://sun.library.msstate.edu/ETD-db/theses/available/etd-04022012-085153/ ;.

Council of Science Editors:

Payne AI. RESPONSE OF LISTERIA MONOCYTOGENES TO BILE SALTS. [Masters Thesis]. Mississippi State University; 2012. Available from: http://sun.library.msstate.edu/ETD-db/theses/available/etd-04022012-085153/ ;

Cornell University

9. Allen, Krystal. The Gnrh Receptor-Associated Membrane Raft Proteome In Mouse Gonadotrope Cells.

Degree: PhD, Veterinary Medicine, 2012, Cornell University

URL: http://hdl.handle.net/1813/31077

► Gonadotropin releasing hormone (GnRH) is the central hormone of reproduction in vertebrates. This hormone is secreted from the hypothalamus in response to environmental, steroid hormone… (more)

Subjects/Keywords: Gonadotropin Releasing Hormone Receptor; Proteomic; Membrane Raft

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APA (6^th Edition):

Allen, K. (2012). The Gnrh Receptor-Associated Membrane Raft Proteome In Mouse Gonadotrope Cells. (Doctoral Dissertation). Cornell University. Retrieved from http://hdl.handle.net/1813/31077

Chicago Manual of Style (16^th Edition):

Allen, Krystal. “The Gnrh Receptor-Associated Membrane Raft Proteome In Mouse Gonadotrope Cells.” 2012. Doctoral Dissertation, Cornell University. Accessed January 23, 2021. http://hdl.handle.net/1813/31077.

MLA Handbook (7^th Edition):

Allen, Krystal. “The Gnrh Receptor-Associated Membrane Raft Proteome In Mouse Gonadotrope Cells.” 2012. Web. 23 Jan 2021.

Vancouver:

Allen K. The Gnrh Receptor-Associated Membrane Raft Proteome In Mouse Gonadotrope Cells. [Internet] [Doctoral dissertation]. Cornell University; 2012. [cited 2021 Jan 23]. Available from: http://hdl.handle.net/1813/31077.

Council of Science Editors:

Allen K. The Gnrh Receptor-Associated Membrane Raft Proteome In Mouse Gonadotrope Cells. [Doctoral Dissertation]. Cornell University; 2012. Available from: http://hdl.handle.net/1813/31077

10. Venkata, Saijyothi Aluru. Proteomic Profiling of Tear fluid for Potential Biomarker Discovery in Dry Eye Syndrome; -.

Degree: Bilogical Sciene, 2014, INFLIBNET

URL: http://shodhganga.inflibnet.ac.in/handle/10603/26180

►

Human tears contain large number of proteins exerting significant influence on tear newlinefilm stability ocular surface integrity and visual function Proteins secreted by the newlinelacrimal… (more)

▼

Human tears contain large number of proteins exerting significant influence on tear newlinefilm stability ocular surface integrity and visual function Proteins secreted by the newlinelacrimal glands have been shown to contribute to the dynamics of the tear film in newlineboth health and disease The possible mediators of lacrimal gland insufficiency in newlineDES includes increased levels of pro inflammatory cytokines production of auto newlineantibodies apoptosis alterations in signaling molecules hormonal imbalance and newlinemany others Therefore alterations in the proteins profile are indicative of the newlinedisease mechanism and identification of marker protein can give clues on the disease newlineseverity as well as on the underlying pathology Proteomic study using mass newlinespectrometric analysis to identify protein biomarkers further linking it to the disease newlineactivity as well as the treatment responses have been reported However there are newlinelimited studies using tear as a specimen to identify such biomarkers Dry eye newlinesyndrome DES is multi factorial diseases of the tears and ocular surface that newlineresults in symptoms of discomfort visual disturbance and tear film instability with newlinepotential damage to the ocular surface It is accompanied with increased osmolarity newlineof the tear film and inflammation Tear acts as good specimen that is non invasively newlineobtained to look for the tear protein changes in DES Moreover the prevalence of newlineDES is increasing and is ranging from 108 to 571 worldwide while the treatment newlineprotocol just involves management by using tear lubricants that are visco elastic newlineagents with no other valuable components and therefore warrants attention globally newlineTherefore the proposed study is to look for the differential expression of tear newlineproteins in DES which has a potential outcome to develop diagnostics as well as newlinetherapeutics The proposed study collected tear using Schirmers strip in the newlinerecruited DES cases as per clinical guidelines and criterions As prospective casecontrol newlinestudy age and sex matched control n73 mean age 43 ± 12 y 30 M 43 F newlineand DES cases n129 mean a

-

Advisors/Committee Members: Angayarkanni, N.

Subjects/Keywords: Eye Syndrome; Potential Biomarker; Proteomic; Tear fluid

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APA (6^th Edition):

Venkata, S. A. (2014). Proteomic Profiling of Tear fluid for Potential Biomarker Discovery in Dry Eye Syndrome; -. (Thesis). INFLIBNET. Retrieved from http://shodhganga.inflibnet.ac.in/handle/10603/26180

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Venkata, Saijyothi Aluru. “Proteomic Profiling of Tear fluid for Potential Biomarker Discovery in Dry Eye Syndrome; -.” 2014. Thesis, INFLIBNET. Accessed January 23, 2021. http://shodhganga.inflibnet.ac.in/handle/10603/26180.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Venkata, Saijyothi Aluru. “Proteomic Profiling of Tear fluid for Potential Biomarker Discovery in Dry Eye Syndrome; -.” 2014. Web. 23 Jan 2021.

Vancouver:

Venkata SA. Proteomic Profiling of Tear fluid for Potential Biomarker Discovery in Dry Eye Syndrome; -. [Internet] [Thesis]. INFLIBNET; 2014. [cited 2021 Jan 23]. Available from: http://shodhganga.inflibnet.ac.in/handle/10603/26180.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Venkata SA. Proteomic Profiling of Tear fluid for Potential Biomarker Discovery in Dry Eye Syndrome; -. [Thesis]. INFLIBNET; 2014. Available from: http://shodhganga.inflibnet.ac.in/handle/10603/26180

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Waterloo

11. Ho, Raymond. Proteomic Analysis of Chinese Hamster Ovary Cells Producing Glycosylated Monoclonal Antibodies.

Degree: 2013, University of Waterloo

URL: http://hdl.handle.net/10012/7622

► Therapeutic monoclonal antibodies (MAb) are produced as secreted complex glycoproteins from mammalian cell systems and represent one of the most important classes of therapeutic medicines… (more)

▼ Therapeutic monoclonal antibodies (MAb) are produced as secreted complex glycoproteins from mammalian cell systems and represent one of the most important classes of therapeutic medicines for the treatment of a variety of human diseases. Their benefit in health care and high economic impact provide the driving force for the development of improved production levels with the focus of optimizing clinical efficacy. One important issue is the optimization of monoclonal antibody production. A frequent approach used to address this challenge is the engineering of mammalian cell lines to increase antibody production levels through genetic manipulation. Valuable information can then be obtained by monitoring the effects of genetic changes on the biochemistry of the cell associated with MAb production. Global protein expression profiling of mammalian cells used for the production of biopharmaceuticals may reveal key biochemical characteristics associated with MAb-producing cell lines. A better understanding of these characteristics can in turn lead to more rational strategies for cell line and process development. The proposed research relates to a larger NSERC Strategic Network (MAbNet) Grant to develop and establish a novel platform for the large-scale manufacture of specific glycoforms of therapeutic monoclonal antibodies. The efficacy of these recombinant MAbs will be enhanced by the control of their glycosylation profiles. The work presented in this thesis will assist MAbNet in meeting their objectives. Specifically, we use 2D-Differential In-Gel Electrophoresis (2D-DIGE) to quantify protein expression differences between EG2-hFc1-producing Chinese Hamster Ovary cells (CHO-1A7) with its parental cell line (CHO-BRI). Here, we identified 34 unique differentially expressed proteins associated with EG2-hFc1 production that relate to various biological processes including protein processing, carbohydrate metabolism, amino acid metabolism, energy metabolism, apoptosis, and cell proliferation pathways. The majority of identified significant protein expression changes and their associated metabolic processes seem to prioritize energy production in CHO-1A7 cells. Due to the metabolic load of recombinant antibody production, the CHO-1A7 cell line attempts to meet the energy requirements needed for recombinant protein biosynthesis while maintaining cell viability and efficient protein folding mechanisms. A 2-D proteome reference map was also constructed for the CHO-BRI host cell line containing 131 identified protein spots. The map provides information that will further expand our understanding of this particular cell line. It will be a useful tool for studies investigating physiological responses and protein expression patterns of CHO-BRI to genetic and environmental perturbations. The set of identified differentially expressed proteins provides data on the downstream changes in protein expression due to genetic manipulation, and furthermore can provide targets for cell-line specific optimization of antibody…

Subjects/Keywords: chinese hamster ovary; proteomic; glycosylation; monoclonal antibody

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Ho, R. (2013). Proteomic Analysis of Chinese Hamster Ovary Cells Producing Glycosylated Monoclonal Antibodies. (Thesis). University of Waterloo. Retrieved from http://hdl.handle.net/10012/7622

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Ho, Raymond. “Proteomic Analysis of Chinese Hamster Ovary Cells Producing Glycosylated Monoclonal Antibodies.” 2013. Thesis, University of Waterloo. Accessed January 23, 2021. http://hdl.handle.net/10012/7622.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Ho, Raymond. “Proteomic Analysis of Chinese Hamster Ovary Cells Producing Glycosylated Monoclonal Antibodies.” 2013. Web. 23 Jan 2021.

Vancouver:

Ho R. Proteomic Analysis of Chinese Hamster Ovary Cells Producing Glycosylated Monoclonal Antibodies. [Internet] [Thesis]. University of Waterloo; 2013. [cited 2021 Jan 23]. Available from: http://hdl.handle.net/10012/7622.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Ho R. Proteomic Analysis of Chinese Hamster Ovary Cells Producing Glycosylated Monoclonal Antibodies. [Thesis]. University of Waterloo; 2013. Available from: http://hdl.handle.net/10012/7622

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Newcastle

12. Ewen, Katherine. Large-scale proteomic screen and signal transduction analysis used to study embryonic gonad and germ cell development.

Degree: PhD, 2010, University of Newcastle

URL: http://hdl.handle.net/1959.13/807499

►

Research Doctorate - Doctor of Philosophy (PhD)

In the fields of sex determination, embryonic gonad development and germ cell differentiation, much effort has been placed… (more)

▼

Research Doctorate - Doctor of Philosophy (PhD)

In the fields of sex determination, embryonic gonad development and germ cell differentiation, much effort has been placed in performing large-scale screens anchored in RNA and DNA technologies. Whilst these technologies have identified new candidate genes, they are unable to provide expression data for functionally active gene products. Recent improvements in the sensitivity of proteomic screening technologies have made analysis of the embryonic gonadal proteome experimentally feasible. Thus, major aims of my PhD were to generate a data set of gonadal proteins expressed at the time of sex determination, and to identify differentially expressed proteins that potentially regulate early events in gonadogenesis, germ cell development and sex differentiation. To detect and identify gonadal proteins, I used two-dimensional nano-flow liquid chromatography and tandem mass spectrometry. I identified 1037 proteins which primarily serve in RNA post-transcriptional modification and trafficking, protein synthesis and folding, and post-translational modification. Over 300 proteins were not identified in a similar transcriptomic study. Over 60 proteins were identified with potential links to human disorders of sexual development (DSDs). I identified four proteins up-regulated in the ovary and three proteins up-regulated in the testis at a critical time point in sex determination. I also identified five proteins increasing, and three proteins decreasing, in expression during early testis development. Two of these temporally-regulated proteins are associated with human DSDs. The majority of these expression differences have not been detected at the transcript level. These data provide novel targets potentially controlling events in gonadogenesis, sex determination and germ cell differentiation. Recently, the field of germ cell sex differentiation was revolutionised with the discovery that retinoic acid (RA) initiates meiosis in female embryonic germ cells, and that the RA-degrading enzyme CYP26B1 inhibits meiosis in male germ cells, which consequently cease mitotic division till after birth. How these somatic environmental factors regulate the transition from mitosis to meiosis or mitotic arrest remains unanswered. p38 MAP kinase (MAPK) signalling initiates mitotic arrest in other differentiating cell types. Thus, a specific aim of my PhD was to investigate the role of p38 MAPK in controlling male germ cell differentiation. To address this aim experimentally, I analysed expression of p38 MAPK in embryonic gonads and found it to be activated in differentiating male germ cells. I then blocked p38 MAPK signalling and found that male germ cells expressed pluripotency and meiosis-associated proteins in a similar pattern to their female counterparts, and that testes exhibited more meiotic germ cells. These data suggest that p38 MAPK signalling contributes to meiosis inhibition in testicular germ cells, and potentially directs them towards quiescence. The studies outlined in this thesis…

Advisors/Committee Members: University of Newcastle. Faculty of Science and Information Technology, School of Environmental and Life Sciences.

Subjects/Keywords: gonad; germ cell; proteomic; p38 MAPK

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APA (6^th Edition):

Ewen, K. (2010). Large-scale proteomic screen and signal transduction analysis used to study embryonic gonad and germ cell development. (Doctoral Dissertation). University of Newcastle. Retrieved from http://hdl.handle.net/1959.13/807499

Chicago Manual of Style (16^th Edition):

Ewen, Katherine. “Large-scale proteomic screen and signal transduction analysis used to study embryonic gonad and germ cell development.” 2010. Doctoral Dissertation, University of Newcastle. Accessed January 23, 2021. http://hdl.handle.net/1959.13/807499.

MLA Handbook (7^th Edition):

Ewen, Katherine. “Large-scale proteomic screen and signal transduction analysis used to study embryonic gonad and germ cell development.” 2010. Web. 23 Jan 2021.

Vancouver:

Ewen K. Large-scale proteomic screen and signal transduction analysis used to study embryonic gonad and germ cell development. [Internet] [Doctoral dissertation]. University of Newcastle; 2010. [cited 2021 Jan 23]. Available from: http://hdl.handle.net/1959.13/807499.

Council of Science Editors:

Ewen K. Large-scale proteomic screen and signal transduction analysis used to study embryonic gonad and germ cell development. [Doctoral Dissertation]. University of Newcastle; 2010. Available from: http://hdl.handle.net/1959.13/807499

University of Illinois – Chicago

13. Zeng, Qi. Proteomic Analysis of Individual Drosophila Hemolymph.

Degree: 2015, University of Illinois – Chicago

URL: http://hdl.handle.net/10027/19413

► Developing analysis methods and tools to understand the chemical information of the proteome of an organism of limited volume are challenging and vital for modern… (more)

▼ Developing analysis methods and tools to understand the chemical information of the proteome of an organism of limited volume are challenging and vital for modern proteomic studies. Drosophila melanogaster (also called fruit fly) is one of the ideal animal models for the study of proteomic composition because of the high degree of genome homology with the human genome. Nevertheless, it has less than 50 nL blood (hemolymph) available for collection due to its small size (about 3 mm). The goal of this thesis is to present sample preparation and analysis methods to improve the identification of proteins in volume-limited biological samples using a series of chromatography and mass spectrometry methods. A hyphenated nano-reverse phase liquid chromatography chip column-mass spectrometry (nano-RPLC chip column-MS) method was developed to obtain proteomic information of hemolymph from an individual fruit fly. This is the first study to report a qualitative analysis of proteomic composition of hemolymph from individual adult female fruit flies. A microliter-scale protein digestion protocol was also developed to assist the digestion efficiency of limited volume sample. With the improved sample preparation method, six novel proteins were identified for the first time at the translation level. Detection of 13 proteins that are well-known in the literature speaks to the method’s validity and demonstrates the ability to reproducibly analyze volume-limited samples from individual fruit flies for protein content. Further, comprehensive prefractionation methods were developed by fabricating 2-cm long chromatography columns for individual fruit fly hemolymph samples. Detection of lower abundance proteins was enhanced with reverse phase and ion exchange prefractionation methods when followed by high mass resolution and high mass accuracy fourier transform ion cyclotron resonance-mass spectrometry (FT-ICR) and Orbitrap mass spectrometers. CE was brought on-line connected with a portable ion trap mass spectrometer by developing an interface. The sheathless CE-MS hyphenated instrument, built in house, enables efficient separation and detection of a list of amino acid standards and provides an alternative fast separation and detection method for small molecule analysis. This coupled CE-MS instrument compliments the complicated FT-ICR tandem mass spectrometry identification for larger molecular weight protein samples. Advisors/Committee Members: Shippy, Scott (advisor), Keiderling, Timothy (committee member), Fung, Leslie (committee member), Hanley, Luke (committee member), Orenic, Teresa (committee member).

Subjects/Keywords: Analytical chemistry; Proteomic analysis; Drosophila hemolymph

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APA (6^th Edition):

Zeng, Q. (2015). Proteomic Analysis of Individual Drosophila Hemolymph. (Thesis). University of Illinois – Chicago. Retrieved from http://hdl.handle.net/10027/19413

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Zeng, Qi. “Proteomic Analysis of Individual Drosophila Hemolymph.” 2015. Thesis, University of Illinois – Chicago. Accessed January 23, 2021. http://hdl.handle.net/10027/19413.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Zeng, Qi. “Proteomic Analysis of Individual Drosophila Hemolymph.” 2015. Web. 23 Jan 2021.

Vancouver:

Zeng Q. Proteomic Analysis of Individual Drosophila Hemolymph. [Internet] [Thesis]. University of Illinois – Chicago; 2015. [cited 2021 Jan 23]. Available from: http://hdl.handle.net/10027/19413.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Zeng Q. Proteomic Analysis of Individual Drosophila Hemolymph. [Thesis]. University of Illinois – Chicago; 2015. Available from: http://hdl.handle.net/10027/19413

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Cambridge

14. Quarantotti, Valentina. Towards the understanding of pericentriolar satellite biology.

Degree: PhD, 2018, University of Cambridge

URL: https://www.repository.cam.ac.uk/handle/1810/274539

► Pericentriolar satellites (PS) are electron dense granules surrounding the centrosome, the major microtubule-organizing centre in eukaryotic cells. In cycling cells the centrosome promotes spindle assembly… (more)

▼ Pericentriolar satellites (PS) are electron dense granules surrounding the centrosome, the major microtubule-organizing centre in eukaryotic cells. In cycling cells the centrosome promotes spindle assembly and the faithful execution of mitosis. In non-cycling cells it is involved in forming the cilium, a plasma membrane-resident organelle, which mediates crucial signalling pathways in development and tissue homeostasis. PS are thought to contribute to centrosome formation, through the microtubule-dependent transport of centrosome components, and they are involved in ciliogenesis and stress response. Moreover, several proteins that localize to PS are mutated in human ciliopathies and neurodevelopmental disorders. The precise roles of PS in the various molecular pathways and diseases are however poorly understood, in part due to the limited knowledge of their composition. In the first part of my study I performed a comprehensive analysis of the pericentriolar satellite proteome. This was achieved by sucrose sedimentation of PS, combined with affinity purification of a key PS component, PCM1. To eliminate contamination by centrosomes, the PS proteome was determined from wild-type cells as well as from two cell lines genetically engineered to lack centrosomes. Mass spectrometry identified 170 PS components including most of the previously described PS proteins, confirming the validity of the approach. Having determined the proteomic composition of PS from DT40 cells, I then performed validation studies both in chicken and human cell lines. In the second part of my study, I aimed to use the list of PS proteins to uncover new biological roles for pericentriolar satellites. I devised two distinct approaches to gain functional insights. First, I generated a cell line lacking PCM1 as a tool to study the role(s) of PS and PS components. Second, I performed loss-of-function studies on a set of new PS proteins to determine their function(s) in maintaining the canonical PS distribution and in forming primary cilia.

Subjects/Keywords: centrosome; centriole; pericentriolar satellite; PCM1; proteomic composition

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APA (6^th Edition):

Quarantotti, V. (2018). Towards the understanding of pericentriolar satellite biology. (Doctoral Dissertation). University of Cambridge. Retrieved from https://www.repository.cam.ac.uk/handle/1810/274539

Chicago Manual of Style (16^th Edition):

Quarantotti, Valentina. “Towards the understanding of pericentriolar satellite biology.” 2018. Doctoral Dissertation, University of Cambridge. Accessed January 23, 2021. https://www.repository.cam.ac.uk/handle/1810/274539.

MLA Handbook (7^th Edition):

Quarantotti, Valentina. “Towards the understanding of pericentriolar satellite biology.” 2018. Web. 23 Jan 2021.

Vancouver:

Quarantotti V. Towards the understanding of pericentriolar satellite biology. [Internet] [Doctoral dissertation]. University of Cambridge; 2018. [cited 2021 Jan 23]. Available from: https://www.repository.cam.ac.uk/handle/1810/274539.

Council of Science Editors:

Quarantotti V. Towards the understanding of pericentriolar satellite biology. [Doctoral Dissertation]. University of Cambridge; 2018. Available from: https://www.repository.cam.ac.uk/handle/1810/274539

Université Catholique de Louvain

15. Ambroise, Jérôme. Contribution of biostatistical methods to genomic and proteomic data analysis : a case for microarray data analysis, transcriptional network inference, and protein binding site detection.

Degree: 2012, Université Catholique de Louvain

URL: http://hdl.handle.net/2078.1/109684

►

The main scope of this thesis is the application of biostatistical methods to genomic and proteomic data analyses. Each part deals specifically with one bioinformatics… (more)

Subjects/Keywords: Bioinformatics; Biostatistics; Genomic; Transcriptomic; Proteomic; Microarray

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APA (6^th Edition):

Ambroise, J. (2012). Contribution of biostatistical methods to genomic and proteomic data analysis : a case for microarray data analysis, transcriptional network inference, and protein binding site detection. (Thesis). Université Catholique de Louvain. Retrieved from http://hdl.handle.net/2078.1/109684

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Ambroise, Jérôme. “Contribution of biostatistical methods to genomic and proteomic data analysis : a case for microarray data analysis, transcriptional network inference, and protein binding site detection.” 2012. Thesis, Université Catholique de Louvain. Accessed January 23, 2021. http://hdl.handle.net/2078.1/109684.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Ambroise, Jérôme. “Contribution of biostatistical methods to genomic and proteomic data analysis : a case for microarray data analysis, transcriptional network inference, and protein binding site detection.” 2012. Web. 23 Jan 2021.

Vancouver:

Ambroise J. Contribution of biostatistical methods to genomic and proteomic data analysis : a case for microarray data analysis, transcriptional network inference, and protein binding site detection. [Internet] [Thesis]. Université Catholique de Louvain; 2012. [cited 2021 Jan 23]. Available from: http://hdl.handle.net/2078.1/109684.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Ambroise J. Contribution of biostatistical methods to genomic and proteomic data analysis : a case for microarray data analysis, transcriptional network inference, and protein binding site detection. [Thesis]. Université Catholique de Louvain; 2012. Available from: http://hdl.handle.net/2078.1/109684

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

16. Collins, Cassandra. A Genomic and Proteomic Investigation of the Plant Pathogen Armillaria mellea: Buried Treasure or Hidden Threat?.

Degree: 2013, RIAN

URL: http://eprints.maynoothuniversity.ie/4513/

► Armillaria mellea is a major plant pathogen of timber and agronomic crops. Yet, no large-scale “-omics” data are available to enable new studies, and limited… (more)

▼ Armillaria mellea is a major plant pathogen of timber and agronomic crops. Yet, no large-scale “-omics” data are available to enable new studies, and limited experimental models are available to investigate basidiomycete pathogenicity. Herein it is revealed that the A. mellea genome comprises 58.35 Mb, contains 14473 gene models, of average length 1575 bp (4.72 introns/gene). A novel, large-scale proteomic shotgun analysis method, was developed for high-throughput proteomics, applicable to other Armillaria spp. and basidiomycete studies. Tandem mass spectrometry identified 951 mycelial (n = 629 unique) and secreted (n = 183 unique) proteins from A. mellea. Almost 100 mycelial proteins were either species-specific or previously unidentified at the protein level. Signal sequence occurrence was 4-fold greater for secreted (50.2%) compared to mycelial (12%) proteins. Analyses revealed a rich reservoir of carbohydrate degrading enzymes, laccases, lignin peroxidases and large cytochrome P450ome in the A. mellea proteome, reminiscent of both basidiomycete and ascomycete glycodegradative arsenals. Under oxidative stress, A. mellea underwent extensive proteome remodelling within 3 hours and comparative proteomics detected differentially regulated proteins (n = 14). Expression of proteins involved in methionine and polyamine biosynthesis (n = 2) and proteins (n = 2) involved maintenance of cellular homeostasis by regulation of homocysteine levels were significantly upregulated. This response may mitigate against oxidative cellular damage. Proteins (n = 2) putatively involved in the regulation of biosynthesis of specific polyamines were also identified. Remarkably, A. mellea exhibits a specific and significant killing effect against Candida albicans during co-culture. Proteomic investigation of this interaction revealed 205 secreted proteins, with the unique expression of defensive and potentially offensive A. mellea proteins (n = 30). Four proteins expressed in co-cultures were specific to A. mellea, or previously unidentified either by homology or at the protein level. Overall, the data reveal new insights into the origin of basidiomycete virulence, and a new model system for further studies aimed at deciphering fungal pathogenic mechanisms is presented.

Subjects/Keywords: Biology; Genomic; Proteomic; Plant Pathogen; Armillaria mellea

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APA (6^th Edition):

Collins, C. (2013). A Genomic and Proteomic Investigation of the Plant Pathogen Armillaria mellea: Buried Treasure or Hidden Threat?. (Thesis). RIAN. Retrieved from http://eprints.maynoothuniversity.ie/4513/

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Collins, Cassandra. “A Genomic and Proteomic Investigation of the Plant Pathogen Armillaria mellea: Buried Treasure or Hidden Threat?.” 2013. Thesis, RIAN. Accessed January 23, 2021. http://eprints.maynoothuniversity.ie/4513/.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Collins, Cassandra. “A Genomic and Proteomic Investigation of the Plant Pathogen Armillaria mellea: Buried Treasure or Hidden Threat?.” 2013. Web. 23 Jan 2021.

Vancouver:

Collins C. A Genomic and Proteomic Investigation of the Plant Pathogen Armillaria mellea: Buried Treasure or Hidden Threat?. [Internet] [Thesis]. RIAN; 2013. [cited 2021 Jan 23]. Available from: http://eprints.maynoothuniversity.ie/4513/.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Collins C. A Genomic and Proteomic Investigation of the Plant Pathogen Armillaria mellea: Buried Treasure or Hidden Threat?. [Thesis]. RIAN; 2013. Available from: http://eprints.maynoothuniversity.ie/4513/

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Iowa State University

17. Harischandra, Hiruni. Filarial nematode exosome-like vesicles (ELVs): Functional significance and control relevance.

Degree: 2017, Iowa State University

URL: https://lib.dr.iastate.edu/etd/16720

► Lymphatic filariasis (LF) is a neglected tropical disease that affects over 120 million people worldwide and is caused by three filarial nematodes including Brugia malayi.… (more)

▼ Lymphatic filariasis (LF) is a neglected tropical disease that affects over 120 million people worldwide and is caused by three filarial nematodes including Brugia malayi. Despite mass drug administration (MDA) to populations at risk for over 17 years, LF still remains a global health concern. Parasitism depends on specific interactions between the parasite and host. Our efforts of understanding these intricate interactions revealed a novel mechanism through which Brugia actively delivers small regulatory RNA and proteins to the host tissues via exosomes-like vesicles (ELV). Proteomics reveal stage and gender specific cargo, including proteins with potential immunomodulatory capacity. This suggests not only stage specific modulation of the host but also potential sexual dimorphism, and supports the hypothesis that these vesicles are released by the parasite to cater to its survival within the host. We have shown that these ELVs are functional and elicit a stage specific response in host macrophages, further supporting the above hypothesis. Further exploration of the possibility of exploiting these ELVs in disease control efforts revealed that exosomal release in these parasites is inhibited by current anthelmintics used to treat LF including ivermectin (IVM). Previous studies suggest a host component in parasite clearance by IVM and the fact that ELV release is inhibited by IVM not only supports this hypothesis but also provides evidence for how such a mechanism might take place. If our hypothesis that ELVs are released by these parasites to immunomodulate the host to aid in survival within the host is true, then inhibition of these vesicle by IVM leaves the parasites vulnerable to host defense mechanisms, thereby allowing effective parasite clearance. The use of ELV release inhibition as a platform for screening novel drugs resulted in the identification of a panel of L-type calcium channel inhibitors that block exosomal release in these parasites more potently than IVM. Current diagnostic methods for LF are suboptimal and here we demonstrate proof of principle of using miRNA as biomarkers for more accurate diagnosis. Collectively our results reveal a novel mechanism in Brugia and demonstrate the potential of exploiting it in a wide spectrum of control efforts.

Subjects/Keywords: Brugia malayi; Exosome; GPCR; Proteomic; Genetics; Parasitology

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APA (6^th Edition):

Harischandra, H. (2017). Filarial nematode exosome-like vesicles (ELVs): Functional significance and control relevance. (Thesis). Iowa State University. Retrieved from https://lib.dr.iastate.edu/etd/16720

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Harischandra, Hiruni. “Filarial nematode exosome-like vesicles (ELVs): Functional significance and control relevance.” 2017. Thesis, Iowa State University. Accessed January 23, 2021. https://lib.dr.iastate.edu/etd/16720.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Harischandra, Hiruni. “Filarial nematode exosome-like vesicles (ELVs): Functional significance and control relevance.” 2017. Web. 23 Jan 2021.

Vancouver:

Harischandra H. Filarial nematode exosome-like vesicles (ELVs): Functional significance and control relevance. [Internet] [Thesis]. Iowa State University; 2017. [cited 2021 Jan 23]. Available from: https://lib.dr.iastate.edu/etd/16720.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Harischandra H. Filarial nematode exosome-like vesicles (ELVs): Functional significance and control relevance. [Thesis]. Iowa State University; 2017. Available from: https://lib.dr.iastate.edu/etd/16720

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

18. Collins, Cassandra. A Genomic and Proteomic Investigation of the Plant Pathogen Armillaria mellea: Buried Treasure or Hidden Threat?.

Degree: 2013, RIAN

URL: http://mural.maynoothuniversity.ie/4513/

► Armillaria mellea is a major plant pathogen of timber and agronomic crops. Yet, no large-scale “-omics” data are available to enable new studies, and limited… (more)

▼ Armillaria mellea is a major plant pathogen of timber and agronomic crops. Yet, no large-scale “-omics” data are available to enable new studies, and limited experimental models are available to investigate basidiomycete pathogenicity. Herein it is revealed that the A. mellea genome comprises 58.35 Mb, contains 14473 gene models, of average length 1575 bp (4.72 introns/gene). A novel, large-scale proteomic shotgun analysis method, was developed for high-throughput proteomics, applicable to other Armillaria spp. and basidiomycete studies. Tandem mass spectrometry identified 951 mycelial (n = 629 unique) and secreted (n = 183 unique) proteins from A. mellea. Almost 100 mycelial proteins were either species-specific or previously unidentified at the protein level. Signal sequence occurrence was 4-fold greater for secreted (50.2%) compared to mycelial (12%) proteins. Analyses revealed a rich reservoir of carbohydrate degrading enzymes, laccases, lignin peroxidases and large cytochrome P450ome in the A. mellea proteome, reminiscent of both basidiomycete and ascomycete glycodegradative arsenals. Under oxidative stress, A. mellea underwent extensive proteome remodelling within 3 hours and comparative proteomics detected differentially regulated proteins (n = 14). Expression of proteins involved in methionine and polyamine biosynthesis (n = 2) and proteins (n = 2) involved maintenance of cellular homeostasis by regulation of homocysteine levels were significantly upregulated. This response may mitigate against oxidative cellular damage. Proteins (n = 2) putatively involved in the regulation of biosynthesis of specific polyamines were also identified. Remarkably, A. mellea exhibits a specific and significant killing effect against Candida albicans during co-culture. Proteomic investigation of this interaction revealed 205 secreted proteins, with the unique expression of defensive and potentially offensive A. mellea proteins (n = 30). Four proteins expressed in co-cultures were specific to A. mellea, or previously unidentified either by homology or at the protein level. Overall, the data reveal new insights into the origin of basidiomycete virulence, and a new model system for further studies aimed at deciphering fungal pathogenic mechanisms is presented.

Subjects/Keywords: Biology; Genomic; Proteomic; Plant Pathogen; Armillaria mellea

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APA (6^th Edition):

Collins, C. (2013). A Genomic and Proteomic Investigation of the Plant Pathogen Armillaria mellea: Buried Treasure or Hidden Threat?. (Thesis). RIAN. Retrieved from http://mural.maynoothuniversity.ie/4513/

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Collins, Cassandra. “A Genomic and Proteomic Investigation of the Plant Pathogen Armillaria mellea: Buried Treasure or Hidden Threat?.” 2013. Thesis, RIAN. Accessed January 23, 2021. http://mural.maynoothuniversity.ie/4513/.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Collins, Cassandra. “A Genomic and Proteomic Investigation of the Plant Pathogen Armillaria mellea: Buried Treasure or Hidden Threat?.” 2013. Web. 23 Jan 2021.

Vancouver:

Collins C. A Genomic and Proteomic Investigation of the Plant Pathogen Armillaria mellea: Buried Treasure or Hidden Threat?. [Internet] [Thesis]. RIAN; 2013. [cited 2021 Jan 23]. Available from: http://mural.maynoothuniversity.ie/4513/.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Collins C. A Genomic and Proteomic Investigation of the Plant Pathogen Armillaria mellea: Buried Treasure or Hidden Threat?. [Thesis]. RIAN; 2013. Available from: http://mural.maynoothuniversity.ie/4513/

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Louisville

19. Lominadze, George, 1978-. Defining the mechanisms of neutrophil exocytosis using proteomic techniques.

Degree: PhD, 2005, University of Louisville

URL: 10.18297/etd/852 ; https://ir.library.louisville.edu/etd/852

► Exocytosis of intracellular granules is critical for conversion of inactive, circulating neutrophils to fully activated cells. The p38 MAPK pathway plays a central role in… (more)

Subjects/Keywords: Neutrophil; Exocytosis; Proteomic

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APA (6^th Edition):

Lominadze, George, 1. (2005). Defining the mechanisms of neutrophil exocytosis using proteomic techniques. (Doctoral Dissertation). University of Louisville. Retrieved from 10.18297/etd/852 ; https://ir.library.louisville.edu/etd/852

Chicago Manual of Style (16^th Edition):

Lominadze, George, 1978-. “Defining the mechanisms of neutrophil exocytosis using proteomic techniques.” 2005. Doctoral Dissertation, University of Louisville. Accessed January 23, 2021. 10.18297/etd/852 ; https://ir.library.louisville.edu/etd/852.

MLA Handbook (7^th Edition):

Lominadze, George, 1978-. “Defining the mechanisms of neutrophil exocytosis using proteomic techniques.” 2005. Web. 23 Jan 2021.

Vancouver:

Lominadze, George 1. Defining the mechanisms of neutrophil exocytosis using proteomic techniques. [Internet] [Doctoral dissertation]. University of Louisville; 2005. [cited 2021 Jan 23]. Available from: 10.18297/etd/852 ; https://ir.library.louisville.edu/etd/852.

Council of Science Editors:

Lominadze, George 1. Defining the mechanisms of neutrophil exocytosis using proteomic techniques. [Doctoral Dissertation]. University of Louisville; 2005. Available from: 10.18297/etd/852 ; https://ir.library.louisville.edu/etd/852

University of Sydney

20. Bernhard, Oliver Karl. Proteomic Investigation of the HIV Receptors CD4 and DC-Sign/CD209 .

Degree: 2004, University of Sydney

URL: http://hdl.handle.net/2123/585

► HIV infection and disease is a multistage process that involves a variety of cell types as the virus spreads through the body. Initially, dendritic cells… (more)

▼ HIV infection and disease is a multistage process that involves a variety of cell types as the virus spreads through the body. Initially, dendritic cells (DCs) present at the mucosal site of infection bind and internalise HIV for degradation and presentation to T cells. As the DCs migrate to lymph nodes and mature, part of the internalised virions remains infective inside endosomal compartments. During formation of the immunological synapse between CD4 T cells and DCs, infective virions from dendritic cells are transferred to CD4 T cells leading to a strong infection of those cells allowing rapid virus dissemination throughout the body and establishment of the typical HIV infection. Various membrane receptors are involved in this process. Initial HIV binding to DCs is mediated by C-type lectin receptors such as the mannose receptor or DC-SIGN (DC specific intracellular adhesion molecule 3 grabbing non integrin) which is followed by virus internalisation and lysis albeit virus induced changes in endocytic routing prevents a proportion from degradation. Productive infection of DCs has also been observed allowing trans infection of CD4 T cells through a different mechanism. HIV infection of CD4 T cells, DCs and other cells is a multistep process initiated by binding of HIV envelope gp120 to the CD4 receptor, a 55 kDa transmembrane glycoprotein. Subsequent conformational changes in gp120 allow binding to a chemokine receptor, either CCR5 or CXCR4, followed by membrane fusion and infection. The aim of this thesis was to investigate protein associations with the HIV receptors DC-SIGN and CD4 in order to elucidate the mechanism of complex formation, virus entry and/or defining target sites for antiretroviral drugs. This thesis used a proteomic approach for studying the receptors with mass spectrometry-based protein identification as its core technology. A range of different approaches were developed and compared for identification of protein interactions and characterisation of the identified protein associations. An affinity purification of the CD4 receptor complex from lymphoid cells was used as the basis for detecting novel CD4-binding proteins. For this approach a strategy based on mass spectrometry identification of CD4 associating proteins using affinity chromatography and affinity-tag mediated purification of tryptic peptides was developed. This method proved successful for the identification of CD4 interacting proteins such as the strongly associated kinase p56lck, however a limited number of non-specifically bound proteins were also identified along the receptor complex. Using one-dimensional SDS-polyacrylamide gel electrophoresis followed by in-gel digests and mass spectrometry analysis, a large number of non-specifically binding proteins were identified along the CD4/lck complex. Evaluation of different lysis buffers in several independent experiments demonstrated that there was a large and inconsistent array of proteins that were obviously non-specifically bound to the receptor. No further specific binding…

Subjects/Keywords: Proteomic; HIV Receptors

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Bernhard, O. K. (2004). Proteomic Investigation of the HIV Receptors CD4 and DC-Sign/CD209 . (Thesis). University of Sydney. Retrieved from http://hdl.handle.net/2123/585

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Bernhard, Oliver Karl. “Proteomic Investigation of the HIV Receptors CD4 and DC-Sign/CD209 .” 2004. Thesis, University of Sydney. Accessed January 23, 2021. http://hdl.handle.net/2123/585.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Bernhard, Oliver Karl. “Proteomic Investigation of the HIV Receptors CD4 and DC-Sign/CD209 .” 2004. Web. 23 Jan 2021.

Vancouver:

Bernhard OK. Proteomic Investigation of the HIV Receptors CD4 and DC-Sign/CD209 . [Internet] [Thesis]. University of Sydney; 2004. [cited 2021 Jan 23]. Available from: http://hdl.handle.net/2123/585.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Bernhard OK. Proteomic Investigation of the HIV Receptors CD4 and DC-Sign/CD209 . [Thesis]. University of Sydney; 2004. Available from: http://hdl.handle.net/2123/585

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Sydney

21. Bali, Hana. Synergism from combination of platinum drugs and selected phytochemicals in colorectal cancer .

Degree: 2018, University of Sydney

URL: http://hdl.handle.net/2123/18798

► Colorectal cancer is the second most leading cause of death among all reported cancer mortality. Chemotherapy is the treatment of choice to treat metastasized colorectal… (more)

Subjects/Keywords: colorectal cancer; platinum drugs; phytochemical; proteomic

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Bali, H. (2018). Synergism from combination of platinum drugs and selected phytochemicals in colorectal cancer . (Thesis). University of Sydney. Retrieved from http://hdl.handle.net/2123/18798

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Bali, Hana. “Synergism from combination of platinum drugs and selected phytochemicals in colorectal cancer .” 2018. Thesis, University of Sydney. Accessed January 23, 2021. http://hdl.handle.net/2123/18798.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Bali, Hana. “Synergism from combination of platinum drugs and selected phytochemicals in colorectal cancer .” 2018. Web. 23 Jan 2021.

Vancouver:

Bali H. Synergism from combination of platinum drugs and selected phytochemicals in colorectal cancer . [Internet] [Thesis]. University of Sydney; 2018. [cited 2021 Jan 23]. Available from: http://hdl.handle.net/2123/18798.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Bali H. Synergism from combination of platinum drugs and selected phytochemicals in colorectal cancer . [Thesis]. University of Sydney; 2018. Available from: http://hdl.handle.net/2123/18798

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

22. Paulo Costa Carvalho. Reconhecimento de padrões proteômicos e genômicos por aprendizagem de máquinas para o disgnóstico médico.

Degree: 2005, Fundação Oswaldo Cruz

URL: http://www.bdtd.cict.fiocruz.br/tedesimplificado/tde_busca/arquivo.php?codArquivo=5

► Objetivo: Aplicar aprendizagem de máquinas para revelar padrões moleculares criptografados no nosso perfil proteômico / genômico humano para auxilio no diagnóstico médico personalizado. Resultados e… (more)

▼ Objetivo: Aplicar aprendizagem de máquinas para revelar padrões moleculares criptografados no nosso perfil proteômico / genômico humano para auxilio no diagnóstico médico personalizado. Resultados e conclusões: 1. Estudos referentes ao perfil proteômico: Foi realizada uma comparação entre o perfil proteômico do soro de pacientes com doença de Hodgkin (HD) e de teóricos saudáveis (CS), com o intuito de buscar padrões moleculares diferencialmente expressos para a obtenção de diagnóstico personalizado. Inicialmente, foi feito um gel unidimensional que revelou duas bandas sobre-expressas (~26 e 18 kDa) em pacientes com HD (p<0.01). Posteriormente, por espectrometria de massa (electrospray - ESI), foram obtidos espectros oriundos do soro de 30 CS e 30 pacientes com HD. Foi implementado um classificador baseado em máquinas de vetor de suporte (SVM) que classificou corretamente todos os espectros como pertencentes ao seu respectivo conjunto baseandose no método de validação cruzada leave-one-out. Um novo algorítmo intitulado analise por divergência máxima (MDA) foi utilizado no espectro multicarga para rastrear biomarcadores. Com apenas dois marcadores, já era possível classificar corretamente 97% de todos os indivíduos. Acreditamos que esta foi a primeira vez que SVM foi aplicado a espectros ESI para fins de diagnóstico médico. Uma nova abordagem para problemas multi-classe que denominamos máquina de clusterização por elipsóides (ECM) foi utilizada para selecionar regiões no espectro livre dos efeitos da doença de Hodgkin e mapeá-los no espaço de características. Todos os CS e pacientes com HD foram corretamente classificados pelo teste de validação cruzada leave-one-out. As fronteiras elípticas aqui modeladas poderiam representar uma definição geométrica de um soro controle. Eventualmente, ao assimilar novos biomarcadores, acreditamos que o modelo poderá ser utilizado para o diagnóstico simultâneo de diversos tipos de câncer. 2. Análise de perfis genéticos: Neste estudo é demonstrada uma abordagem computacional para avaliação personalizada da severidade de hipertensão, combinando marcadores genômicos associados ao sistema reninaangeotensina- aldosterona (RAAS) e dados clínicos. Os marcadores genômicos mais relevantes para a tomada de decisão são prontamente selecionados de um banco de dados, com combinações pré-computadas relativas ao genótipo e dados clínicos do indivíduo, para um diagnóstico personalizado. O risco da doença é avaliado por modelo gerado a partir da técnica de vetor de suporte, e depois é mensurada a distância Euclidiana até a fronteira de decisão do modelo. Para gerar este banco de dados, uma nova metodologia híbrida de seleção de características foi utilizada, tendo como conjunto de treinamento dados clínicos e genômicos de brasileiros hipertensos e normotensos. A seleção de características aplicada aos dados aplótipos RAAS confirmaram a sua associação com a hipertensão e elucidaram padrões distintos de polimorfismo para grupos com diferentes etnias. 3. Computação distribuída A maioria das soluções grid… Advisors/Committee Members: Wim Maurits Sylvain Degrave, Gilberto Barbosa Domont.

Subjects/Keywords: BIOLOGIA MOLECULAR; Análise de perfis genéticos; Computação distribuída; Perfil proteômico; Proteomic profile studies; Genetic profile analysis; Proteomic profile

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Carvalho, P. C. (2005). Reconhecimento de padrões proteômicos e genômicos por aprendizagem de máquinas para o disgnóstico médico. (Thesis). Fundação Oswaldo Cruz. Retrieved from http://www.bdtd.cict.fiocruz.br/tedesimplificado/tde_busca/arquivo.php?codArquivo=5

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Carvalho, Paulo Costa. “Reconhecimento de padrões proteômicos e genômicos por aprendizagem de máquinas para o disgnóstico médico.” 2005. Thesis, Fundação Oswaldo Cruz. Accessed January 23, 2021. http://www.bdtd.cict.fiocruz.br/tedesimplificado/tde_busca/arquivo.php?codArquivo=5.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Carvalho, Paulo Costa. “Reconhecimento de padrões proteômicos e genômicos por aprendizagem de máquinas para o disgnóstico médico.” 2005. Web. 23 Jan 2021.

Vancouver:

Carvalho PC. Reconhecimento de padrões proteômicos e genômicos por aprendizagem de máquinas para o disgnóstico médico. [Internet] [Thesis]. Fundação Oswaldo Cruz; 2005. [cited 2021 Jan 23]. Available from: http://www.bdtd.cict.fiocruz.br/tedesimplificado/tde_busca/arquivo.php?codArquivo=5.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Carvalho PC. Reconhecimento de padrões proteômicos e genômicos por aprendizagem de máquinas para o disgnóstico médico. [Thesis]. Fundação Oswaldo Cruz; 2005. Available from: http://www.bdtd.cict.fiocruz.br/tedesimplificado/tde_busca/arquivo.php?codArquivo=5

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

23. Ngo, Chinh Quoc. Proteomic profiling of metalloenzymes via robust coordination of active-site metal.

Degree: MA, Chemistry, 2018, University of Texas – Austin

URL: http://hdl.handle.net/2152/63543

► Proteomic profiling of metalloenzymes in their native state is possible without using a covalent crosslinking group, if the interaction between the metal-binding group of the… (more)

Subjects/Keywords: Proteomic; Carbonic anhydrase; Proteomic profiling; Metalloenzymes

…native metal-protein interaction and/or metalation state.10 Traditional, bottom-up proteomic… …complicates analysis. False metalation states caused by incompatible proteomic techniques need to be… …later adopted for the proteomic labeling of metalloproteases (MPs), Zn-containing… …Proteomic profiling of HDACs, another very important class of Zn-containing enzymes, was achieved… …on proteomic profiling of carbonic anhydrase (CA).17,18 The probe design…

4 more images…

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APA (6^th Edition):

Ngo, C. Q. (2018). Proteomic profiling of metalloenzymes via robust coordination of active-site metal. (Masters Thesis). University of Texas – Austin. Retrieved from http://hdl.handle.net/2152/63543

Chicago Manual of Style (16^th Edition):

Ngo, Chinh Quoc. “Proteomic profiling of metalloenzymes via robust coordination of active-site metal.” 2018. Masters Thesis, University of Texas – Austin. Accessed January 23, 2021. http://hdl.handle.net/2152/63543.

MLA Handbook (7^th Edition):

Ngo, Chinh Quoc. “Proteomic profiling of metalloenzymes via robust coordination of active-site metal.” 2018. Web. 23 Jan 2021.

Vancouver:

Ngo CQ. Proteomic profiling of metalloenzymes via robust coordination of active-site metal. [Internet] [Masters thesis]. University of Texas – Austin; 2018. [cited 2021 Jan 23]. Available from: http://hdl.handle.net/2152/63543.

Council of Science Editors:

Ngo CQ. Proteomic profiling of metalloenzymes via robust coordination of active-site metal. [Masters Thesis]. University of Texas – Austin; 2018. Available from: http://hdl.handle.net/2152/63543

NSYSU

24. Hsu, Cheng-Chieh. Study of CYP26B1 and associated proteins in betel quid induced oral cancers.

Degree: Master, Institute of Biomedical Sciences, 2013, NSYSU

URL: http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0615113-102942

► Approximately 600 million people in the world chew betel quid (BQ). In 2004, the International Agency for Research on Cancer (IARC) declared BQ chewing without… (more)

Subjects/Keywords: Betel Quid; Arecoline; CYP26B1; Areca nut; Oral cancer; Proteomic

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APA (6^th Edition):

Hsu, C. (2013). Study of CYP26B1 and associated proteins in betel quid induced oral cancers. (Thesis). NSYSU. Retrieved from http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0615113-102942

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Hsu, Cheng-Chieh. “Study of CYP26B1 and associated proteins in betel quid induced oral cancers.” 2013. Thesis, NSYSU. Accessed January 23, 2021. http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0615113-102942.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Hsu, Cheng-Chieh. “Study of CYP26B1 and associated proteins in betel quid induced oral cancers.” 2013. Web. 23 Jan 2021.

Vancouver:

Hsu C. Study of CYP26B1 and associated proteins in betel quid induced oral cancers. [Internet] [Thesis]. NSYSU; 2013. [cited 2021 Jan 23]. Available from: http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0615113-102942.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Hsu C. Study of CYP26B1 and associated proteins in betel quid induced oral cancers. [Thesis]. NSYSU; 2013. Available from: http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0615113-102942

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

UCLA

25. Quach, Austin. Determinants of the epigenetic clock.

Degree: Human Genetics, 2018, UCLA

URL: http://www.escholarship.org/uc/item/0w5293jn

► It has been observed that the epigenome exhibits significant changes with increased age. These changes are so consistent that they have been used to develop… (more)

Subjects/Keywords: Aging; Analytical chemistry; aging; epigenetic clock; LC-MS; metabolomic; proteomic; transcriptomic

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APA (6^th Edition):

Quach, A. (2018). Determinants of the epigenetic clock. (Thesis). UCLA. Retrieved from http://www.escholarship.org/uc/item/0w5293jn

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Quach, Austin. “Determinants of the epigenetic clock.” 2018. Thesis, UCLA. Accessed January 23, 2021. http://www.escholarship.org/uc/item/0w5293jn.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Quach, Austin. “Determinants of the epigenetic clock.” 2018. Web. 23 Jan 2021.

Vancouver:

Quach A. Determinants of the epigenetic clock. [Internet] [Thesis]. UCLA; 2018. [cited 2021 Jan 23]. Available from: http://www.escholarship.org/uc/item/0w5293jn.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Quach A. Determinants of the epigenetic clock. [Thesis]. UCLA; 2018. Available from: http://www.escholarship.org/uc/item/0w5293jn

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Tasmania

26. Alharbi, MAS. Aging and recovery of Listeria monocytogenes ScottA.

Degree: 2018, University of Tasmania

URL: https://eprints.utas.edu.au/28360/1/Alharbi_whole_thesis.pdf

► The bacterial growth curve is considered to involve four phases: lag, exponential, stationary and death/decline phase. The lag phase is the time that bacteria use… (more)

▼ The bacterial growth curve is considered to involve four phases: lag, exponential, stationary and death/decline phase. The lag phase is the time that bacteria use to recover and adapt to a new environment before shifting to exponential growth at a rate determined by the environmental conditions and available resources. Aging, on the other hand, is the process in which the inheritance of “new” and “old” cell poles by bacterial cells during cell division as well as putative accumulation of protein aggregates. Stress has been shown to be needed to make aging inevitable, and aging leads to cells that lose fitness thus providing a finite duration to a stressed population. Significant knowledge gaps still exist about the lag phase and bacterial aging, in particular, mechanistic details of the processes and whether broad scale principals apply. The thesis study aims to describe the microbiological, biochemical and regulatory-level mechanisms of the effect of cell aging and lag phase recovery in Listeria monocytogenes (strain Scott A). L. monocytogenes was utilised in the study due to its comparatively streamlined genome, high level of genetic and protein-level characterisation and the fact it is an important food pathogen that causes the disease listeriosis. The species is adept at switching its lifestyle from an environmental saprophyte to a human parasite and thus has a capacity to respond and adapt to relatively hostile environments. In the environment, it can survive due to this high degree of adaptability, and this has resulted in a ubiquitous distribution. How it adapts in the scenarios of enforced aging and subsequent recovery was approached by mainly using quantitative proteomics, allowing assessment of most essential cell functions. To enable understanding of the effect of culture-based aging on L. monocytogenes ScottA growth and survival behaviour, morphological and metabolic changes, virulence potential, and ATP levels during aging processes were assessed. Aging was enforced by holding cells at 37°C deep into the stationary growth phase for up to 21 days. The temperature used represents the optimal temperature for growth rate (T(_opt)). In older cultures (aged =≥7 d) lag phase was proceeded by possible short, rapid recovery of a small segment (0.5-1.5 log units) of the population. The duration of the lag phase increased with the length of time the cells were initially incubated. The generation time of outgrowth increased with the duration of initial incubation suggesting more aged cells lost fitness. Extended incubation resulted in increasingly poor growth on agar. Aged cultures lose the ability to retain crystal violet, indicative of a thinner, less networked peptidoglycan layer. They also demonstrated cell elongation (2-10 fold increase), evidence of failed septation completion, and autolysis. After 7 d incubation, ATP levels in the cells collapsed to <10(^-17) moles/cell from approximately 10(^-15) moles/cell. Catabolic enzymes associated with protein, lipid, phosphate, and carbohydrate metabolism…

Subjects/Keywords: Listeria; aging; proteomic; recovery; regulon; lag phase; stationary phase

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Alharbi, M. (2018). Aging and recovery of Listeria monocytogenes ScottA. (Thesis). University of Tasmania. Retrieved from https://eprints.utas.edu.au/28360/1/Alharbi_whole_thesis.pdf

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Alharbi, MAS. “Aging and recovery of Listeria monocytogenes ScottA.” 2018. Thesis, University of Tasmania. Accessed January 23, 2021. https://eprints.utas.edu.au/28360/1/Alharbi_whole_thesis.pdf.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Alharbi, MAS. “Aging and recovery of Listeria monocytogenes ScottA.” 2018. Web. 23 Jan 2021.

Vancouver:

Alharbi M. Aging and recovery of Listeria monocytogenes ScottA. [Internet] [Thesis]. University of Tasmania; 2018. [cited 2021 Jan 23]. Available from: https://eprints.utas.edu.au/28360/1/Alharbi_whole_thesis.pdf.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Alharbi M. Aging and recovery of Listeria monocytogenes ScottA. [Thesis]. University of Tasmania; 2018. Available from: https://eprints.utas.edu.au/28360/1/Alharbi_whole_thesis.pdf

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Alberta

27. Saleem,fozia. The use of modern metabolomics and proteomics to address the health challenges facing the Canadian cattle industry.

Degree: PhD, Department of Biological Sciences, 2013, University of Alberta

URL: https://era.library.ualberta.ca/files/v979v447g

► Naturally cattle only consumed grass, hay and other forage crops but modern cattle industry has started shifting them from a natural grazing diet to a… (more)

Subjects/Keywords: prion diseases; lipopoysaccharides; Health challenges, cattle, metabolomics, proteomic, LPS, BSE

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APA (6^th Edition):

Saleem,fozia. (2013). The use of modern metabolomics and proteomics to address the health challenges facing the Canadian cattle industry. (Doctoral Dissertation). University of Alberta. Retrieved from https://era.library.ualberta.ca/files/v979v447g

Note: this citation may be lacking information needed for this citation format:
Author name may be incomplete

Chicago Manual of Style (16^th Edition):

Saleem,fozia. “The use of modern metabolomics and proteomics to address the health challenges facing the Canadian cattle industry.” 2013. Doctoral Dissertation, University of Alberta. Accessed January 23, 2021. https://era.library.ualberta.ca/files/v979v447g.

Note: this citation may be lacking information needed for this citation format:
Author name may be incomplete

MLA Handbook (7^th Edition):

Saleem,fozia. “The use of modern metabolomics and proteomics to address the health challenges facing the Canadian cattle industry.” 2013. Web. 23 Jan 2021.

Note: this citation may be lacking information needed for this citation format:
Author name may be incomplete

Vancouver:

Saleem,fozia. The use of modern metabolomics and proteomics to address the health challenges facing the Canadian cattle industry. [Internet] [Doctoral dissertation]. University of Alberta; 2013. [cited 2021 Jan 23]. Available from: https://era.library.ualberta.ca/files/v979v447g.

Note: this citation may be lacking information needed for this citation format:
Author name may be incomplete

Council of Science Editors:

Saleem,fozia. The use of modern metabolomics and proteomics to address the health challenges facing the Canadian cattle industry. [Doctoral Dissertation]. University of Alberta; 2013. Available from: https://era.library.ualberta.ca/files/v979v447g

Note: this citation may be lacking information needed for this citation format:
Author name may be incomplete

Université Montpellier II

28. Batxelli, Isabelle. Recherche d'un profil protéique corrélé aux encéphalopathies spongiformes subaigües transmissibles (ESST) : analyses en spectrométrie de masse SELDI-TOF : Search of a proteic profile correlated to the TSEs by SELDI-TOF MS technology.

Degree: Docteur es, Neurosciences, 2010, Université Montpellier II

URL: http://www.theses.fr/2010MON20127

►

Les encéphalopathies spongiformes subaigües transmissibles (ESST) sont des maladies neurodégénératives affectant l'Homme et les animaux, l'issue est toujours fatale. La détection dans le sang de… (more)

▼

Les encéphalopathies spongiformes subaigües transmissibles (ESST) sont des maladies neurodégénératives affectant l'Homme et les animaux, l'issue est toujours fatale. La détection dans le sang de l'agent pathogène responsable de l'infection (protéine prion pathologique)reste difficile à ce jour et l'identification de nouveaux biomarqueurs impliqués dans la physiopathologie des ESST constitue un projet ambitieux et risqué. Dans ce contexte, notre objectif est d'établir un profil protéique corrélé aux ESST. L'utilisation d'un modèle animalbien caractérisé : la tremblante naturelle du mouton, d'une technologie adaptée à l'analyse de profils protéiques : SELDI-TOF MS et d'un fluide biologique : le sérum, a constitué la base de nos travaux de recherche. Dans un premier temps, les protocoles expérimentaux ont été mis en place et optimisés. Puis, ils ont été évalués pour leur pertinence dans la discrimination de moutons pathologiques en phases précoce et tardive de la maladie versus des moutons contrôles par analyse des sérums fractionnés ou non. Des biomarqueurs potentiels de faibles poids moléculaires ont été sélectionnés à l'aide de la méthode statistique SAM et une signature protéique permettant un diagnostic précoce a été établie (87% de sensibilité et 90%de spécificité). Un des biomarqueurs a été identifié comme étant un fragment de la transthyrétine, puis son potentiel discriminant a été évalué en SELDI-TOF MS dans une étude cinétique de hamsters Syriens infectés par la scrapie, en western blot et par dosage ELISA.Finalement, une cohorte de validation constituée de moutons appelés « scrapie-free » a permis de valider les biomarqueurs les plus pertinents.

Transmissible spongiform encephalopathies (TSEs) are fatal neurodegenerative diseasesoccurring in animals and humans for which no ante-mortem diagnostic test in biological fluidsis available. In such pathologies, detection of the pathological form of the prion protein (i.e.,the causative factor) in blood is difficult. Identification of new biomarkers implicated in thepathway of prion infection is relevant. In this context, our objective was to find a proteicprofile correlated to TSEs. We used a well-known TSE model: scrapie in sheep breeding, amass spectrometry technology easy-to-use for proteic profiling: SELDI-TOF MS and abiological fluid: serum. First, experimental tools have been developed and optimized. Thesetools were evaluated for their discriminating potential of control sheep and animals with earlyor late phase scrapie using a large number of serum samples (fractionated or not). Then, usingthe SAM statistical method, potential low molecular weight biomarkers were selected. Amongthese biomarkers, a protein signature pattern was identified; it can discriminate between earlyphase scrapie and control sera (sensitivity of 87% and specificity of 90%). One of theseproteins was identified as a fragment of transthyretin and evaluated as a biomarker using aSELDI-TOF MS kinetic study of sera from scrapie infected Syrian hamsters. This biomarkerwas also confirmed by…

Advisors/Committee Members: Mourton, Chantal (thesis director).

Subjects/Keywords: Biomarqueurs; Sang; Protéomique; Esst; Scrapie; Biomarker; Blood; Proteomic; TSEs; Scrapie

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Batxelli, I. (2010). Recherche d'un profil protéique corrélé aux encéphalopathies spongiformes subaigües transmissibles (ESST) : analyses en spectrométrie de masse SELDI-TOF : Search of a proteic profile correlated to the TSEs by SELDI-TOF MS technology. (Doctoral Dissertation). Université Montpellier II. Retrieved from http://www.theses.fr/2010MON20127

Chicago Manual of Style (16^th Edition):

Batxelli, Isabelle. “Recherche d'un profil protéique corrélé aux encéphalopathies spongiformes subaigües transmissibles (ESST) : analyses en spectrométrie de masse SELDI-TOF : Search of a proteic profile correlated to the TSEs by SELDI-TOF MS technology.” 2010. Doctoral Dissertation, Université Montpellier II. Accessed January 23, 2021. http://www.theses.fr/2010MON20127.

MLA Handbook (7^th Edition):

Batxelli, Isabelle. “Recherche d'un profil protéique corrélé aux encéphalopathies spongiformes subaigües transmissibles (ESST) : analyses en spectrométrie de masse SELDI-TOF : Search of a proteic profile correlated to the TSEs by SELDI-TOF MS technology.” 2010. Web. 23 Jan 2021.

Vancouver:

Batxelli I. Recherche d'un profil protéique corrélé aux encéphalopathies spongiformes subaigües transmissibles (ESST) : analyses en spectrométrie de masse SELDI-TOF : Search of a proteic profile correlated to the TSEs by SELDI-TOF MS technology. [Internet] [Doctoral dissertation]. Université Montpellier II; 2010. [cited 2021 Jan 23]. Available from: http://www.theses.fr/2010MON20127.

Council of Science Editors:

Batxelli I. Recherche d'un profil protéique corrélé aux encéphalopathies spongiformes subaigües transmissibles (ESST) : analyses en spectrométrie de masse SELDI-TOF : Search of a proteic profile correlated to the TSEs by SELDI-TOF MS technology. [Doctoral Dissertation]. Université Montpellier II; 2010. Available from: http://www.theses.fr/2010MON20127

University of Tasmania

29. Feng, Shi. Is Proteorhodopsin a general light-driven stress adaptation system for survival in cold environments.

Degree: 2014, University of Tasmania

URL: https://eprints.utas.edu.au/22486/1/front-Shi-thesis.pdf ; https://eprints.utas.edu.au/22486/2/Whole-Shi-thesis.pdf

► This study aimed to achieve a better understanding of microbial adaptations in sea ice focusing on the physiological role of the light harvesting proton pump… (more)

▼ This study aimed to achieve a better understanding of microbial adaptations in sea ice focusing on the physiological role of the light harvesting proton pump proteorhodopsin. To carry out these aims the research mainly focused on exploring the genome biology, physiology and life strategy of the model sea-ice bacterial species Psychroflexus torquis, an extremely psychrophilic member of the family Flavobacteriaceae (phylum Bacteroidetes). P. torquis has a bipolar distribution and is only known to occur in polar sea-ice and associated polar waters. It possesses proteorhodopsin and is believed to have a predominantly epiphytic lifestyle, mainly dwelling on sea-ice diatoms in sea-ice basal assemblages. This study extensively used gel-free label-free based proteomic approach to explore P. torquis’ genome biology and unravel the role of proteorhodopsin in aiding the species adaptation to the extreme sea ice environment. Sea ice has been estimated to have only become a stable feature on Earth in the last few million years ago thus it has been hypothesized that bacteria adapted to sea-ice acquired or exchange survival traits via horizontal gene transfer (HGT) between other sea ice dwelling microorganisms relatively recently. To examine the question whether sea-ice bacteria, such as P. torquis are endemic and display sea ice-ecosystem specialism a comparison of P. torquis’ genome to its very closely related (99% 16S rRNA gene sequence similarity) sister species, P. gondwanensis ACAM 44T, which is only known to dwell in Antarctic hypersaline lakes, was performed. This comparison allowed for the determination of the level of HGT, what traits show evidence of HGT, what traits are relevant to the sea-ice ecosystem, and whether these genes are highly expressed, which would be indicative of their biological importance to P. torquis. The results show that in P. torquis ATCC 700755T (genome size 4.3 Mbp) HGT has occurred much more extensively compared to P. gondwanensis (genome size 3.3 Mbp) and genetic features that can be linked as a sea ice specific adaptation are mainly concentrated on numerous genomic islands absent from P. gondwanensis. Genes encoding sea-ice ecosystem relevant traits, such as secreted exopolysaccharide, poly-unsaturated fatty acids, and ice binding proteins, form gene clusters on a number of these genomic islands. Proteomic analysis revealed that the encoded proteins for many sea-ice relevant traits are highly abundant under standard laboratory growth conditions. The genomic islands feature comparatively low gene density, a high concentration of pseudogenes, repetitive genetic elements, and addiction modules, indicative of large scale HGT either via phage or conjugation driven insertions.The overall results suggest the extensive level and nature of gene acquisition in P. torquis indicates its potential evolution to sea-ice ecosystem specialism. In that respect P. torquis seems to be an excellent model to study sea-ice functional biology. The initial screening of the P. torquis…

Subjects/Keywords: Proteorhodopsin; sea-ice; proteomic; comparative geomics; evolution; life strategy

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Feng, S. (2014). Is Proteorhodopsin a general light-driven stress adaptation system for survival in cold environments. (Thesis). University of Tasmania. Retrieved from https://eprints.utas.edu.au/22486/1/front-Shi-thesis.pdf ; https://eprints.utas.edu.au/22486/2/Whole-Shi-thesis.pdf

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Feng, Shi. “Is Proteorhodopsin a general light-driven stress adaptation system for survival in cold environments.” 2014. Thesis, University of Tasmania. Accessed January 23, 2021. https://eprints.utas.edu.au/22486/1/front-Shi-thesis.pdf ; https://eprints.utas.edu.au/22486/2/Whole-Shi-thesis.pdf.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Feng, Shi. “Is Proteorhodopsin a general light-driven stress adaptation system for survival in cold environments.” 2014. Web. 23 Jan 2021.

Vancouver:

Feng S. Is Proteorhodopsin a general light-driven stress adaptation system for survival in cold environments. [Internet] [Thesis]. University of Tasmania; 2014. [cited 2021 Jan 23]. Available from: https://eprints.utas.edu.au/22486/1/front-Shi-thesis.pdf ; https://eprints.utas.edu.au/22486/2/Whole-Shi-thesis.pdf.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Feng S. Is Proteorhodopsin a general light-driven stress adaptation system for survival in cold environments. [Thesis]. University of Tasmania; 2014. Available from: https://eprints.utas.edu.au/22486/1/front-Shi-thesis.pdf ; https://eprints.utas.edu.au/22486/2/Whole-Shi-thesis.pdf

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

30. Frozza, Caroline Olivieri da Silva. Caracterização química e avaliação da atividade biológica da própolis vermelha em células tumorais e não tumorais.

Degree: 2012, Universidade de Caxias do Sul

URL: https://repositorio.ucs.br/handle/11338/658

► A própolis vermelha brasileira tem atraído interesses científicos e econômicos devido às suas variadas atividades biológicas. Este produto natural possui composição química diferente de acordo… (more)

▼ A própolis vermelha brasileira tem atraído interesses científicos e econômicos devido às suas variadas atividades biológicas. Este produto natural possui composição química diferente de acordo com a região na qual é encontrado, sendo necessária uma completa caracterização química para cada tipo de própolis, a fim de se elucidar os compostos presentes e possivelmente responsáveis por estas atividades. Dentre as atividades biológicas mais investigadas, destacam-se as atividades antioxidante e antitumoral. Neste trabalho, buscou-se caracterizar quimicamente o extrato hidroalcoólico da própolis vermelha brasileira, avaliar as atividades antioxidantes e antitumorais, além de investigar o padrão proteico de células tumorais de laringe tratadas e não tratadas com extratos da própolis vermelha através da análise proteômica comparativa. A caracterização química realizada através de espectrometria de massas com ionização por electrospray mostrou que a própolis apresenta moléculas complexas, principalmente isoflavonoides, compostos com importantes atividades biológicas. Os extratos hidroalcoólicos obtidos a partir da própolis vermelha revelaram um significante conteúdo de polifenóis associados a uma habilidade de sequestrar radicais livres DPPH·. Os extratos também apresentaram atividades superóxido-dismutase-/ike e catalase-/ike, indicando que podem exercer um papel fundamental na manutenção fisiológica do equilíbrio redox quando em um organismo. A atividade citotóxica dos extratos da própolis foi avaliada nas linhagens tumorais Hep-2, HeLa e não tumoral Hek-293, sendo que os valores de IC50 foram menores para a linhagens tumorais em relação a não tumoral. Desta forma, sugere-se uma seletividade da própolis vermelha quanto às linhagens tumorais. A análise proteômica, usando eletroforese bidimensional associada à cromatografia líquida de alta eficiência acoplada a espectrômetro de massa, permitiu a comparação dos mapas proteicos da linhagem Hep-2, na ausência ou presença de extratos da própolis vermelha, nas concentrações 6 f.1g/mL (não citotóxica) e 120 f.lg/mL (IC50). A excisão manual de 325 spots foi efetuada nos géis 2D- SDS-PAGE, em que 177 proteínas foram identificadas. Estas proteínas foram relacionadas com diversos processos metabólicos e estruturais como produção e conversão de energia, transporte e metabolismo de carboidratos, modificação pós-traducional, reciclagem de proteínas e chaperonas, proteínas do citoesqueleto, proteínas de reparo, entre outros. Das proteínas identificadas com expressão diferencial, cinco apresentaram expressão reduzida na presença do extrato da própolis, este em sua maior concentração (120 f.lg/mL). Apenas duas proteínas identificadas neste estudo mostraram expressão aumentada na concentração não citotóxica (6 f.lg/mL) do extrato da própolis vermelha. Os resultados da proteômica comparativa mostram que a própolis interfere em um conjunto de eventos intracelulares e, assim, passa a ser uma candidata promissora para inibir o crescimento celular e contribuir para os diferentes passos… Advisors/Committee Members: Ely, Mariana Roesch, Henriques, João Antonio Pêgas.

Subjects/Keywords: Própole; Tumores; Agentes antineoplásicos; Antioxidantes; Red propolis; Antitumor; Antioxidant; Proteomic

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Frozza, C. O. d. S. (2012). Caracterização química e avaliação da atividade biológica da própolis vermelha em células tumorais e não tumorais. (Masters Thesis). Universidade de Caxias do Sul. Retrieved from https://repositorio.ucs.br/handle/11338/658

Chicago Manual of Style (16^th Edition):

Frozza, Caroline Olivieri da Silva. “Caracterização química e avaliação da atividade biológica da própolis vermelha em células tumorais e não tumorais.” 2012. Masters Thesis, Universidade de Caxias do Sul. Accessed January 23, 2021. https://repositorio.ucs.br/handle/11338/658.

MLA Handbook (7^th Edition):

Frozza, Caroline Olivieri da Silva. “Caracterização química e avaliação da atividade biológica da própolis vermelha em células tumorais e não tumorais.” 2012. Web. 23 Jan 2021.

Vancouver:

Frozza COdS. Caracterização química e avaliação da atividade biológica da própolis vermelha em células tumorais e não tumorais. [Internet] [Masters thesis]. Universidade de Caxias do Sul; 2012. [cited 2021 Jan 23]. Available from: https://repositorio.ucs.br/handle/11338/658.

Council of Science Editors:

Frozza COdS. Caracterização química e avaliação da atividade biológica da própolis vermelha em células tumorais e não tumorais. [Masters Thesis]. Universidade de Caxias do Sul; 2012. Available from: https://repositorio.ucs.br/handle/11338/658

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Open Access Theses and Dissertations