Advanced search options

Advanced Search Options 🞨

Browse by author name (“Author name starts with…”).

Find ETDs with:

in
/  
in
/  
in
/  
in

Written in Published in Earliest date Latest date

Sorted by

Results per page:

Sorted by: relevance · author · university · dateNew search

You searched for subject:(prostacyclin PGI2 ). Showing records 1 – 2 of 2 total matches.

Search Limiters

Last 2 Years | English Only

No search limiters apply to these results.

▼ Search Limiters


University of Houston

1. Mohite, Anita. Effect of Selective Up regulation of Prostacyclin Synthesis On Its Signaling And Vascular Protection.

Degree: Pharmacology, Pharmacology, 2012, University of Houston

Prostacyclin (PGI2) is a very important endogenous vascular protector. It provides vascular protection through actions like vasodilatation, platelet aggregation and vascular smooth muscle cell proliferation inhibition and smooth muscle differentiation regulation. Therefore since decades, there have been many ongoing attempts to use PGI2 to treat vascular disorders such as pulmonary arterial hypertension, peripheral artery disease and thrombosis. However, the short half-life of PGI2 has limited its therapeutic potential. PGI2 is synthesized endogenously from arachidonic acid using enzymes cyclooxygenase isoform 1/2(COX-1/2) and prostacyclin synthase (PGIS). Single gene therapy with either COX or PGIS through adenoviral vectors does not specifically overproduce PGI2 and also is associated with inflammatory reactions. Thus to address these problems, our lab engineered a single hybrid gene (COX-1-10aa-PGIS) by fusing COX-1 with PGIS through transmembrane linker of 10 amino acids (10aa). Adipose tissue derived cells have been used therapeutically in several vascular disorders due to their regenerative properties. Thus we isolated and cultured mature adipocytes (MA) from mouse adipose tissue and called it as prostanoid synthesizing fat cells (PSFC). Further, we engineered a novel adipose tissue-derived cell that constantly produces PGI2, through transfecting of the engineered cDNA of the hybrid enzyme (human COX-1-10-aa-PGIS), which has superior triple catalytic functions in directly converting arachidonic acid into PGI2. The gene-transfected cells were further converted into a stable cell line, in which the cells constantly expressed the hybrid enzyme and were capable of overproducing specifically PGI2 and thus we called this cell line as PGI2 producing PSFC (PGI2-PSFC). When the results of the HPLC activity assay and LC/MS/MS were compared between just vector transfected (PSFC) and COX-1-10aa-PGIS gene transfected cells (PGI2-PSFC), it was observed that the majority of the endogenous AA metabolism shifted from that of unwanted PGE2 (in PSFC) to that of the preferred PGI2 (in PGI2-PSFC) with a PGI2/PGE2 ratio change from 0.03 to 25. The PGI2-producing cell line not only exhibited an approximate 50-fold increase in PGI2 biosynthesis, but also demonstrated superior anti-platelet aggregation in vitro, and increased reperfusion in the mouse ischemic hindlimb and thrombogenesis models in vivo. PGI2 regulates functions like vascular smooth muscle cell differentiation and proliferation and apoptosis. These actions are mediated through the PGI2 receptor (IP) and nuclear receptor, peroxisome proliferator-activated receptors (PPAR). MicroRNAs (miRNAs), which are negative regulators of gene expression, also regulate similar functions like PGI2. PGI2 regulates expression of genes by signaling through PPARs. Thus to find out if PGI2 regulates gene expression by regulating miRNA expression by signaling through the IP and PPAR, a miRNA microarray analysis of the PGI2-PSFCs and PSFCs was performed. Analysis of the results obtained… Advisors/Committee Members: Ruan, Ke-He (advisor), Bond, Richard (committee member), William, Louis (committee member), Gao, Xiaolian (committee member), Yu, Rong (committee member).

Subjects/Keywords: Prostacyclin; COX-1-10aa-PGIS; PSFCs; PGI2-PSFCs, Triple catalytic enzyme-1

Record DetailsSimilar RecordsGoogle PlusoneFacebookTwitterCiteULikeMendeleyreddit

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6th Edition):

Mohite, A. (2012). Effect of Selective Up regulation of Prostacyclin Synthesis On Its Signaling And Vascular Protection. (Doctoral Dissertation). University of Houston. Retrieved from http://hdl.handle.net/10657/249

Chicago Manual of Style (16th Edition):

Mohite, Anita. “Effect of Selective Up regulation of Prostacyclin Synthesis On Its Signaling And Vascular Protection.” 2012. Doctoral Dissertation, University of Houston. Accessed April 16, 2021. http://hdl.handle.net/10657/249.

MLA Handbook (7th Edition):

Mohite, Anita. “Effect of Selective Up regulation of Prostacyclin Synthesis On Its Signaling And Vascular Protection.” 2012. Web. 16 Apr 2021.

Vancouver:

Mohite A. Effect of Selective Up regulation of Prostacyclin Synthesis On Its Signaling And Vascular Protection. [Internet] [Doctoral dissertation]. University of Houston; 2012. [cited 2021 Apr 16]. Available from: http://hdl.handle.net/10657/249.

Council of Science Editors:

Mohite A. Effect of Selective Up regulation of Prostacyclin Synthesis On Its Signaling And Vascular Protection. [Doctoral Dissertation]. University of Houston; 2012. Available from: http://hdl.handle.net/10657/249


Vanderbilt University

2. Bloodworth, Melissa Harintho. Regulation of Immune Responses during Airway Inflammation.

Degree: PhD, Microbiology and Immunology, 2017, Vanderbilt University

Allergic asthma is refractory to corticosteroid treatment in up to 10% of patients and often leads to hospital admissions caused by respiratory viral and/ or bacterial infections. In these studies, I found that: 1) STAT6 inhibited innate γδ17 cell immune responses. STAT6 suppression of γδ17 cell function may provide one explanation for why asthmatic patients have significantly greater risk for invasive bacterial disease, including pneumonia, than nonasthmatic subjects. 2) A GLP-1R agonist, an FDA-approved agent currently used for Type II Diabetes, attenuated the type 2 immune response to RSV and attenuates RSV illness. The current availability of GLP-1R agonists for human treatment highlights the clinical significance of these studies as this therapy could be immediately transferrable to RSV disease. 3) PGI2, an FDA-approved agent currently used for pulmonary hypertension, protected against autoimmunity; enhanced Treg stability and function; rendered T effector cells more susceptible to Treg- mediated suppression; and promoted iTreg differentiation. PGI2 may therefore represent a novel treatment strategy for diseases that result from Treg dysregulation. Advisors/Committee Members: Ray Stokes Peebles Jr., MD (committee member), Wonder Drake, MD (committee member), Joshua P. Fessel, MD, PhD (committee member), Amy S. Major, PhD (committee member), John V. Williams (committee member), David M. Aronoff, MD (Committee Chair).

Subjects/Keywords: type 2 immunity (Th2); gamma-delta 17 (γδ17) cells; STAT6; Klebsiella pneumoniae; glucagon-like peptide 1 (GLP-1); respiratory syncytial virus (RSV); phenome-wide association study (PheWAS); regulatory T cells (Tregs); prostacyclin (PGI2)

Record DetailsSimilar RecordsGoogle PlusoneFacebookTwitterCiteULikeMendeleyreddit

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6th Edition):

Bloodworth, M. H. (2017). Regulation of Immune Responses during Airway Inflammation. (Doctoral Dissertation). Vanderbilt University. Retrieved from http://hdl.handle.net/1803/11246

Chicago Manual of Style (16th Edition):

Bloodworth, Melissa Harintho. “Regulation of Immune Responses during Airway Inflammation.” 2017. Doctoral Dissertation, Vanderbilt University. Accessed April 16, 2021. http://hdl.handle.net/1803/11246.

MLA Handbook (7th Edition):

Bloodworth, Melissa Harintho. “Regulation of Immune Responses during Airway Inflammation.” 2017. Web. 16 Apr 2021.

Vancouver:

Bloodworth MH. Regulation of Immune Responses during Airway Inflammation. [Internet] [Doctoral dissertation]. Vanderbilt University; 2017. [cited 2021 Apr 16]. Available from: http://hdl.handle.net/1803/11246.

Council of Science Editors:

Bloodworth MH. Regulation of Immune Responses during Airway Inflammation. [Doctoral Dissertation]. Vanderbilt University; 2017. Available from: http://hdl.handle.net/1803/11246

.