subject:(progestin)

University of North Texas

1. Paulos, Peter M. Reproductive and Growth Responses of the Fathead Minnow (Pimephales Promelas) and Japanese Medaka (Oryzias Latipes) to the Synthetic Progestin, Norethindrone.

Degree: 2011, University of North Texas

URL: https://digital.library.unt.edu/ark:/67531/metadc68029/

► A commonly prescribed contraceptive, the synthetic progestin norethindrone (NET) inhibits ovulation in humans. However, ecotoxicological data are lacking. Preliminary tests produced an LC50 for NET… (more)

▼ A commonly prescribed contraceptive, the synthetic progestin norethindrone (NET) inhibits ovulation in humans. However, ecotoxicological data are lacking. Preliminary tests produced an LC50 for NET of > 1.0 mg/L (96-hour, fathead minnow (FHM) and medaka) and a NOEC of 242.0 µg/L, a LOEC of 485.0 µg/L (7-day, growth for FHM and medaka). Reproductive testing revealed a LOEC for fecundity of 24.1 ng/L (21 days, medaka). Further testing confirmed the LOEC of 24.1 ng/L while defining a NOEC of 4.7 ng/L (28 days, medaka). Effect of NET in medaka life-cycle exposure at concentrations exceeding 4.7 ng/L was evident. Few females were present in the 24.7 ng/L exposure concentration, with none in the 104.6 ng/L. Egg production was significantly reduced at concentrations exceeding 4.7 ng/L. Additionally, weight, condition factor and somatic indices were significantly different in males exposed to concentrations exceeding 4.7 ng/L. For fecundity and sexual differentiation; the NOEC was 4.7 ng/L, the LOEC 24.6 ng/L; growth and somatic indices, the NOEC was more appropriately 0.9 ng/L, with effect evident at 4.7 ng/L. Sexual differentiation of the F1 population was similar to the F0. A defining result of this test was development of exceptionally large ovaries in NET- exposed female medaka, perhaps indicative of a threshold limit for exposure in these fish. Results of FHM life-cycle testing were similar, establishing a NOEC for fecundity of 0.9 ng/L, a LOEC of 4.8 ng/L. NET's inhibitory effect on gonadal development was obvious; GSI NOEC for males, 4.8 ng/L, and histological examination confirmed the presence of intersex development at elevated concentrations. Normal physical development and growth were impaired, generally at concentrations exceeding 24.1 ng/L. At exposure concentrations exceeding 4.8 ng/L, external sexual confirmation of fish was difficult; LOEC for finspot development in females, 4.8 ng/L. Sexual determination of the 97.1 ng/L exposure group was impossible; externally, all fish appeared male and internal examination revealed no gonadal development. Advisors/Committee Members: La Point, Thomas W., 1949-, Huggett, Duane B., Waller, William T., Venables, Barney J., Dzialowski, Edward M. (Edward Michael).

Subjects/Keywords: Norethindrone; fathead minnow; progestin; endocrine disruption

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Paulos, P. M. (2011). Reproductive and Growth Responses of the Fathead Minnow (Pimephales Promelas) and Japanese Medaka (Oryzias Latipes) to the Synthetic Progestin, Norethindrone. (Thesis). University of North Texas. Retrieved from https://digital.library.unt.edu/ark:/67531/metadc68029/

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Paulos, Peter M. “Reproductive and Growth Responses of the Fathead Minnow (Pimephales Promelas) and Japanese Medaka (Oryzias Latipes) to the Synthetic Progestin, Norethindrone.” 2011. Thesis, University of North Texas. Accessed January 24, 2021. https://digital.library.unt.edu/ark:/67531/metadc68029/.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Paulos, Peter M. “Reproductive and Growth Responses of the Fathead Minnow (Pimephales Promelas) and Japanese Medaka (Oryzias Latipes) to the Synthetic Progestin, Norethindrone.” 2011. Web. 24 Jan 2021.

Vancouver:

Paulos PM. Reproductive and Growth Responses of the Fathead Minnow (Pimephales Promelas) and Japanese Medaka (Oryzias Latipes) to the Synthetic Progestin, Norethindrone. [Internet] [Thesis]. University of North Texas; 2011. [cited 2021 Jan 24]. Available from: https://digital.library.unt.edu/ark:/67531/metadc68029/.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Paulos PM. Reproductive and Growth Responses of the Fathead Minnow (Pimephales Promelas) and Japanese Medaka (Oryzias Latipes) to the Synthetic Progestin, Norethindrone. [Thesis]. University of North Texas; 2011. Available from: https://digital.library.unt.edu/ark:/67531/metadc68029/

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Mississippi State University

2. Wolf, Victoria Lea. A multi-compartment model of the normal menstrual cycle: Integrating hormonal, ovarian, and endometrial elements.

Degree: MS, Agricultural and Biological Engineering, 2014, Mississippi State University

URL: http://sun.library.msstate.edu/ETD-db/theses/available/etd-04032014-143613/ ;

► The uterine endometrium undergoes cyclical phases of cell proliferation, cell differentiation, and menstruation under the influence of the ovarian hormones, estrogen and progesterone. Since… (more)

Subjects/Keywords: progestin-only contraception; menstrual cycle; estrogen; progesterone; endometrium; mathematical model

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Wolf, V. L. (2014). A multi-compartment model of the normal menstrual cycle: Integrating hormonal, ovarian, and endometrial elements. (Masters Thesis). Mississippi State University. Retrieved from http://sun.library.msstate.edu/ETD-db/theses/available/etd-04032014-143613/ ;

Chicago Manual of Style (16^th Edition):

Wolf, Victoria Lea. “A multi-compartment model of the normal menstrual cycle: Integrating hormonal, ovarian, and endometrial elements.” 2014. Masters Thesis, Mississippi State University. Accessed January 24, 2021. http://sun.library.msstate.edu/ETD-db/theses/available/etd-04032014-143613/ ;.

MLA Handbook (7^th Edition):

Wolf, Victoria Lea. “A multi-compartment model of the normal menstrual cycle: Integrating hormonal, ovarian, and endometrial elements.” 2014. Web. 24 Jan 2021.

Vancouver:

Wolf VL. A multi-compartment model of the normal menstrual cycle: Integrating hormonal, ovarian, and endometrial elements. [Internet] [Masters thesis]. Mississippi State University; 2014. [cited 2021 Jan 24]. Available from: http://sun.library.msstate.edu/ETD-db/theses/available/etd-04032014-143613/ ;.

Council of Science Editors:

Wolf VL. A multi-compartment model of the normal menstrual cycle: Integrating hormonal, ovarian, and endometrial elements. [Masters Thesis]. Mississippi State University; 2014. Available from: http://sun.library.msstate.edu/ETD-db/theses/available/etd-04032014-143613/ ;

University of Illinois – Chicago

3. Toh, May Fern. Identification and Biological Characterization of Progestins from Botanicals In Vitro and In Vivo.

Degree: 2014, University of Illinois – Chicago

URL: http://hdl.handle.net/10027/18868

► Extensive studies have been conducted on botanicals for the presence of estrogenic molecules, unfortunately, very little has ever been done to identify plant-based progestins. This… (more)

▼ Extensive studies have been conducted on botanicals for the presence of estrogenic molecules, unfortunately, very little has ever been done to identify plant-based progestins. This underexplored area is troubling especially since there is a body of scientific evidence supporting the presence of progesterone receptor (PR) modulators in plant material that may affect endocrine function. The goal of this dissertation was to identify and characterize natural progesterone (P4)-like compounds from botanical extracts for the improvement of women’s health. Red clover, hops, angelica, black cohosh, kudzu, dogwood, and chaste-tree berry were investigated for their ability to interact with purified PR, to activate PRE-luciferase transcription in human breast cancer cells, and for tissue specific regulation of P4 inducible genes. Kaempferol was identified as having P4-like activity and may function in a cell-specific manner. In vivo studies revealed that kaempferol exhibited P4-like effects in ovariectomized Sprague-Dawley rat model. Since genistein is a phytoestrogen that previously demonstrated to increase uterine weight and proliferation, the ability of kaempferol to block genistein action in the uterus was investigated. Analyses of proliferation, steroid receptor expression, and induction of well-established PR-regulated targets Areg and Hand2 were completed. In addition, kaempferol in silico binding analysis was completed for PR, as was the activation of ER and AR signaling in vitro in order to determine receptor specificity. The data from this dissertation suggest that kaempferol interacts with PR, activates the receptor without stimulating its degradation, induces known PR target genes in vitro and in vivo and blocked genistein-induced proliferation in the luminal epithelial cells in Sprague-Dawley rat uteri. The toxicity of hops extracts in cell-based assays precluded further investigation in vitro, but an initial bioassay screen suggested that natural progestins can be identified from hops extracts. Further chemical and biological analyses are warranted to identify and characterize progestins from hops. The comprehensive framework outlined in this thesis will provide a promising avenue for the identification of potentially better and safer compounds capable of activating PR signaling from botanical extracts used for women's health. Advisors/Committee Members: Burdette, Joanna E. (advisor), Murphy, Brian T. (committee member), Moore, Terry W. (committee member), Thomas, Douglas D. (committee member), Stocco, Carlos D. (committee member).

Subjects/Keywords: Kaempferol; Progestin; Hormone replacement therapy; Genistein; Red clover; Progesterone receptor

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Toh, M. F. (2014). Identification and Biological Characterization of Progestins from Botanicals In Vitro and In Vivo. (Thesis). University of Illinois – Chicago. Retrieved from http://hdl.handle.net/10027/18868

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Toh, May Fern. “Identification and Biological Characterization of Progestins from Botanicals In Vitro and In Vivo.” 2014. Thesis, University of Illinois – Chicago. Accessed January 24, 2021. http://hdl.handle.net/10027/18868.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Toh, May Fern. “Identification and Biological Characterization of Progestins from Botanicals In Vitro and In Vivo.” 2014. Web. 24 Jan 2021.

Vancouver:

Toh MF. Identification and Biological Characterization of Progestins from Botanicals In Vitro and In Vivo. [Internet] [Thesis]. University of Illinois – Chicago; 2014. [cited 2021 Jan 24]. Available from: http://hdl.handle.net/10027/18868.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Toh MF. Identification and Biological Characterization of Progestins from Botanicals In Vitro and In Vivo. [Thesis]. University of Illinois – Chicago; 2014. Available from: http://hdl.handle.net/10027/18868

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

4. Bick, Alexis J. Cross talk between the glucocorticoid receptor and the progesterone receptor in modulation of progestin responses and HIV-1 infection.

Degree: Image, Molecular and Cell Biology, 2018, University of Cape Town

URL: http://hdl.handle.net/11427/28403

► Current epidemiological data showing that the use of the injectable contraceptive progestin Depotmedroxyprogesterone acetate (DMPA) is associated with increased HIV-1 acquisition is controversial. However, animal… (more)

▼ Current epidemiological data showing that the use of the injectable contraceptive progestin Depotmedroxyprogesterone acetate (DMPA) is associated with increased HIV-1 acquisition is controversial. However, animal and ex vivo data reveal plausible biological mechanisms whereby MPA may increase HIV-1 acquisition. Relatively high levels of endogenous progesterone (P4) found in the luteal phase of the menstrual cycle have also been linked to increased HIV-1 acquisition in animal, clinical and ex vivo models. One of the central hypotheses of the present study was that the mechanism of MPA-induced increase in HIV-1 infection occurs via a different mechanism to that of the luteal phase. Furthermore, MPA has been shown to activate both the glucocorticoid receptor (GR) and its target, the progesterone receptor (PR) isoform B (PR-B), which are both transcription factors and regulate genes involved in immune function. Both the GR and PR are expressed in the cervix, the primary site of heterosexual HIV-1 infection. PR is regulated by endogenous estrogen (E2), of which the concentrations fluctuate throughout the menstrual cycle, and GR expression also varies in response to stress hormones, leading to conditions of varied relative levels of GR/PR. The immune-related consequences of changing the relative levels of GR and PR-B are not well understood. Therefore another hypothesis of this study was that changing the relative levels of GR/PR-B modulates HIV-1 infection and immunomodulatory gene expression in response to the GR/PR agonist, MPA. Since GR and PR-B recognize similar DNA target sequences and may regulate the same genes at the same time, the final hypothesis of the present study was that GR and PR-B reciprocally modulate each other’s activity, through possible association. To investigate the effects of exogenous hormones on HIV-1 infection and mechanisms thereof, peripheral blood mononuclear cells (PBMCs) and TZM-bl cervical cells were used as model systems for HIV-1 infection. These cells were stimulated with P4 and E2 at concentrations mimicking the menstrual cycle phases or with levels of MPA at the upper range of peak serum levels detected in DMPA users. Cells were infected with the R-tropic HIV-1 infectious molecular clone, HIV-1Bal_Renilla and luciferase assays were used to measure HIV-1 infection. Levels of HIV-1 CD4 receptor and CCR5 co-receptor protein or mRNA were measured by flow cytometry or qPCR, respectively, while activation of CD4+ T cells using the activation marker CD69 was measured by flow cytometry in PBMCs. To investigate the effects of changing GR/PR-B levels on HIV-1 infection and immune gene regulation, GR/PR levels were altered in End1/E6E7 immortalized endocervical and HeLa/TZM-bl cervical carcinoma cells by GR siRNA knockdown with or without the simultaneous over-expression of PR-B, and cells were stimulated with MPA or the GR agonist Dexamethasone. mRNA expression iii of key immunomodulatory genes in End1/E6E7 and HeLa cells was measured by qPCR. The modulation of GR activity by PR-B was assessed… Advisors/Committee Members: Hapgood, Janet (advisor), Avenant, Chanel (advisor).

Subjects/Keywords: contraceptive progestin Depotmedroxyprogesterone acetate; HIV-1 acquisition; ex vivo data

10 more images…

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Bick, A. J. (2018). Cross talk between the glucocorticoid receptor and the progesterone receptor in modulation of progestin responses and HIV-1 infection. (Thesis). University of Cape Town. Retrieved from http://hdl.handle.net/11427/28403

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Bick, Alexis J. “Cross talk between the glucocorticoid receptor and the progesterone receptor in modulation of progestin responses and HIV-1 infection.” 2018. Thesis, University of Cape Town. Accessed January 24, 2021. http://hdl.handle.net/11427/28403.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Bick, Alexis J. “Cross talk between the glucocorticoid receptor and the progesterone receptor in modulation of progestin responses and HIV-1 infection.” 2018. Web. 24 Jan 2021.

Vancouver:

Bick AJ. Cross talk between the glucocorticoid receptor and the progesterone receptor in modulation of progestin responses and HIV-1 infection. [Internet] [Thesis]. University of Cape Town; 2018. [cited 2021 Jan 24]. Available from: http://hdl.handle.net/11427/28403.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Bick AJ. Cross talk between the glucocorticoid receptor and the progesterone receptor in modulation of progestin responses and HIV-1 infection. [Thesis]. University of Cape Town; 2018. Available from: http://hdl.handle.net/11427/28403

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Adelaide

5. Ochnik, Aleksandra Monica. The molecular actions of medroxyprogesterone acetate on androgen receptor signalling and the promotion of breast cancer.

Degree: 2012, University of Adelaide

URL: http://hdl.handle.net/2440/83570

► The Women’s Health Initiative (WHI) clinical trial was the first randomised, double blind, placebo-controlled disease prevention trial to demonstrate epidemiological evidence of a casual link… (more)

▼ The Women’s Health Initiative (WHI) clinical trial was the first randomised, double blind, placebo-controlled disease prevention trial to demonstrate epidemiological evidence of a casual link between the use of combined hormone replacement therapy (cHRT) comprising conjugated equine estrogens (CEE) and the synthetic progestin medroxyprogesterone acetate (MPA) and increased breast cancer risk in post-menopausal women. Since the first WHI report in 2002, other observational studies have demonstrated that it is the addition of the synthetic progestin in the cHRT that is associated with an increase in breast cancer risk. The focus of this thesis was to investigate the following hypothesis formulated in the Dame Roma Mitchell Cancer Research Laboratory, which proposed that MPA possesses antagonistic actions on androgen receptor (AR)-signalling and thereby can disrupt the protective effect of androgens in the breast, thus leading to an increased risk of breast cancer. Androgens have been associated with a growth restrictive role in breast tissue in both humans and animals and are now emerging as key hormonal pathways involved in the pathogenesis of breast cancer. The objectives of this thesis were firstly to determine the relationship between the use of cHRT containing MPA and breast cancer incidence in Australian women, and secondly to perform biological studies to investigate the effect of AR-action in breast epithelial cells. Initial findings described in this thesis led to the identification of a positive association between the use of cHRT preparations containing MPA and breast cancer incidence in Australian women. Subsequent biological based studies were undertaken with non-malignant breast tissues samples from pre- and post- menopausal women in an ex vivo breast explant tissue culture experimental model and the oestrogen receptor (ER), progesterone receptor (PR) and AR positive ZR-75-1 breast cancer cell line to investigate the actions of MPA on AR-signalling and cancer-related intracellular signalling pathways. Collectively these studies demonstrated that the actions of MPA can impede the anti-proliferative actions of DHT in both human postmenopausal non-malignant and malignant breast epithelial cells via AR-mediated actions. Furthermore, the combined actions of DHT and MPA were also shown to de-regulate cancer-related intracellular pathways compared to individual hormone treatments. The findings described in this thesis provide novel results indicating that MPA may promote the development of breast cancer in post-menopausal women taking cHRT via AR-mediated actions and that use of this form of hormone therapy remains a major public health concern. Advisors/Committee Members: Tilley, Wayne Desmond (advisor), Hickey, Theresa Elizabeth (advisor), School of Medicine (school).

Subjects/Keywords: synthetic progestin; medroxyprogesterone acetate; hormone replacement therapy; breast cancer

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Ochnik, A. M. (2012). The molecular actions of medroxyprogesterone acetate on androgen receptor signalling and the promotion of breast cancer. (Thesis). University of Adelaide. Retrieved from http://hdl.handle.net/2440/83570

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Ochnik, Aleksandra Monica. “The molecular actions of medroxyprogesterone acetate on androgen receptor signalling and the promotion of breast cancer.” 2012. Thesis, University of Adelaide. Accessed January 24, 2021. http://hdl.handle.net/2440/83570.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Ochnik, Aleksandra Monica. “The molecular actions of medroxyprogesterone acetate on androgen receptor signalling and the promotion of breast cancer.” 2012. Web. 24 Jan 2021.

Vancouver:

Ochnik AM. The molecular actions of medroxyprogesterone acetate on androgen receptor signalling and the promotion of breast cancer. [Internet] [Thesis]. University of Adelaide; 2012. [cited 2021 Jan 24]. Available from: http://hdl.handle.net/2440/83570.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Ochnik AM. The molecular actions of medroxyprogesterone acetate on androgen receptor signalling and the promotion of breast cancer. [Thesis]. University of Adelaide; 2012. Available from: http://hdl.handle.net/2440/83570

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Washington

6. Beaber, Elisabeth Frances. Use of oral contraceptives (OC) and breast cancer risk among young women by OC formulation and breast cancer subtype.

Degree: PhD, 2012, University of Washington

URL: http://hdl.handle.net/1773/20539

► Background: Many studies suggest a modest increased breast cancer risk is associated with recent oral contraceptive (OC) use, but risks associated with contemporary OCs and… (more)

▼ Background: Many studies suggest a modest increased breast cancer risk is associated with recent oral contraceptive (OC) use, but risks associated with contemporary OCs and molecular subtypes of breast cancer are less well known. Estrogen and progestin doses have declined and new progestins have been used since OCs were introduced. Furthermore, the vast majority of studies have relied on self-reported OC use, rather than pharmacy data. Methods: Two studies examining invasive breast cancer risk were conducted in western Washington state. The first was a population-based interview case-control study among women ages 20-44 from 2004-2010 (985 cases, 882 controls). Logistic regression was used to estimate associations between lifetime OC use, overall risk, and breast cancer risk by subtype (estrogen receptor positive (ER+), ER-, and triple-negative (ER-/PR-/HER2-)). The second study was a nested case-control study among Group Health Cooperative health plan enrollees. Cases were ages 20-49 at diagnosis from 1990-2009 (n=1102) and controls were randomly selected (n=21952). Associations between recent OC use (within 1 year of reference date) by OC formulation and ER status were evaluated using conditional logistic regression. Results: In the first study, recent OC use for ≥5 years increased breast cancer risk (odds ratio (OR)=1.6), as well as lifetime duration of use for ≥15 years (OR=1.5). There was no statistically significant risk heterogeneity by OC formulation. ORs were generally greater among women ages 20-39. ER- cancer ORs were greater than ER+ ORs. Triple-negative breast cancer ORs were especially elevated. In the second study, recent OC use increased breast cancer risk (OR=1.6). Risk was greater for ER+ (OR=1.7) than ER- cancer (OR=1.3). Risk varied by OC formulation, with some OCs associated with an increased risk and others not associated with risk. Conclusions: We found that recent use of contemporary OCs increases breast cancer risk among women ages 20-49 and that long durations of use increases risk among ages 20-44. Risks may be greater among younger women and for triple-negative breast cancer. Risks may also vary by OC formulation. Continued monitoring of breast cancer risk is essential as OC formulations and use patterns continue to evolve. Advisors/Committee Members: Li, Christopher I (advisor).

Subjects/Keywords: breast cancer; case-control study; estrogen; oral contraceptives; progestin; Epidemiology; Epidemiology

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Beaber, E. F. (2012). Use of oral contraceptives (OC) and breast cancer risk among young women by OC formulation and breast cancer subtype. (Doctoral Dissertation). University of Washington. Retrieved from http://hdl.handle.net/1773/20539

Chicago Manual of Style (16^th Edition):

Beaber, Elisabeth Frances. “Use of oral contraceptives (OC) and breast cancer risk among young women by OC formulation and breast cancer subtype.” 2012. Doctoral Dissertation, University of Washington. Accessed January 24, 2021. http://hdl.handle.net/1773/20539.

MLA Handbook (7^th Edition):

Beaber, Elisabeth Frances. “Use of oral contraceptives (OC) and breast cancer risk among young women by OC formulation and breast cancer subtype.” 2012. Web. 24 Jan 2021.

Vancouver:

Beaber EF. Use of oral contraceptives (OC) and breast cancer risk among young women by OC formulation and breast cancer subtype. [Internet] [Doctoral dissertation]. University of Washington; 2012. [cited 2021 Jan 24]. Available from: http://hdl.handle.net/1773/20539.

Council of Science Editors:

Beaber EF. Use of oral contraceptives (OC) and breast cancer risk among young women by OC formulation and breast cancer subtype. [Doctoral Dissertation]. University of Washington; 2012. Available from: http://hdl.handle.net/1773/20539

7. Perrin-Terrin, Anne-Sophie. Étude de la modulation par l’étonogestrel, l’érythropoïétine et la leptine de la réponse ventilatoire à l'hypercapnie. Détermination de cibles thérapeutiques potentielles pour le traitement des syndromes d’hypoventilation alvéolaire centrale : Study of the modulation by etonogestrel, erythropoietin and leptin of the ventilatory response to hypercapnia. Determination of potential therapeutic targets for the treatment of central alveolar hypoventilation syndromes.

Degree: Docteur es, Santé et Sciences de la vie / Biologie, 2018, Sorbonne Paris Cité

URL: http://www.theses.fr/2018USPCD019

► Les patients atteints de syndromes d’hypoventilation centrale ont une hypoventilation associée à une sensibilité au CO2 réduite ou abolie, au moins pendant le sommeil. A… (more)

▼ Les patients atteints de syndromes d’hypoventilation centrale ont une hypoventilation associée à une sensibilité au CO2 réduite ou abolie, au moins pendant le sommeil. A ce jour, il n’existe pas de traitement pharmacologique (Gozal, 1998, Cielo & Marcus, 2014). Ce constat nous a conduit à étudier les mécanismes de modulation de la commande centrale respiratoire (CCR) de base ou lors d’une hypercapnie par 3 molécules distinctes (i.e. l’étonogestrel, l’érythropoïétine, la leptine) dontl’implication dans la réponse ventilatoire à l’hypercapnie préalablement démontrée ou suggérée a éveillé un intérêt thérapeutique.Dans ce contexte, l’objectif de ces travaux de thèse a constitué d’une part à caractériser l’influence de ces molécules sur la ventilation de base et sur la réponse ventilatoire à l’hypercapnie,d’autre part à déterminer en parallèle leurs mécanismes d’action. Pour aborder cette problématique,nous avons mené nos expérimentations sur des souris sauvages recevant de l’étonogestrel et des souris génétiquement modifiées sous-exprimant l’érythropoïétine (Epo-TAgh) et déficientes en leptine(ob/ob). Afin appréhender les mécanismes centraux mis en jeu par l’ETO en normocapnie, nous avons couplé l’analyse de la CCR lors d’applications pharmacologiques à de l’histologie fonctionnelle sur des préparations ex vivo de bulbe rachidien-moelle épinière isolé de souris nouveau-née. En revanche pour étudier les mécanismes d’actions de l’Epo et la leptine en normocapnie et lors d’une hypercapnie,nous avons couplé une analyse des variables ventilatoires enregistrées par pléthysmographie à de l’histologie fonctionnelle sur des souris ob/ob et Epo-TAgh. Pour ces dernières, une analyse de la CCR sur des préparations ex vivo de bulbe rachidien-moelle épinière isolé de souris nouveau-né Epo-TAgha également été réalisée. Nos données obtenues chez l’animal sauvage sont permis de mettre en évidence un effet dose dépendant de l’ETO sur la fR de base en normocapnie. L’effet global du progestatif semble résulter de la sommation d’un effet facilitateur bulbaire dépendant d’interactions avec des récepteurs GABAA etNMDA et de l’activation des systèmes sérotoninergiques. L’ensemble de nos expériences réalisées sur les souris génétiquement modifiées Epo-TAgh et ob/ob ont permis de conclure que l’Epo ou la leptine n’était pas nécessaire à l’obtention d’une réponse ventilatoire à l’hypercapnie mais qu’un déficit en Epo ou en leptine induisait une modification du patron ventilatoire et du réseau neuronal activé par l’hypercapnie au niveau bulbaire pour l’Epo et également supra-bulbaire et diencéphalique pour la leptine. Nous posons ainsi l’hypothèse que ces modifications soient reliées à une plasticité neuronale importante induite par la déficience en Epo et en leptine. Les données obtenues ouvrent des perspectives quant au fait de déterminer si les mécanismes enclenchés par les progestatifs de la famille des gonanes visant à moduler la CCR pourraient être utilisés comme une alternative thérapeutique pertinente pour le traitement des troubles… Advisors/Committee Members: Voituron, Nicolas (thesis director), Bodineau, Laurence (thesis director).

Subjects/Keywords: Commande centrale respiratoire; Progestatif; Central respiratory drive; Progestin

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Perrin-Terrin, A. (2018). Étude de la modulation par l’étonogestrel, l’érythropoïétine et la leptine de la réponse ventilatoire à l'hypercapnie. Détermination de cibles thérapeutiques potentielles pour le traitement des syndromes d’hypoventilation alvéolaire centrale : Study of the modulation by etonogestrel, erythropoietin and leptin of the ventilatory response to hypercapnia. Determination of potential therapeutic targets for the treatment of central alveolar hypoventilation syndromes. (Doctoral Dissertation). Sorbonne Paris Cité. Retrieved from http://www.theses.fr/2018USPCD019

Chicago Manual of Style (16^th Edition):

Perrin-Terrin, Anne-Sophie. “Étude de la modulation par l’étonogestrel, l’érythropoïétine et la leptine de la réponse ventilatoire à l'hypercapnie. Détermination de cibles thérapeutiques potentielles pour le traitement des syndromes d’hypoventilation alvéolaire centrale : Study of the modulation by etonogestrel, erythropoietin and leptin of the ventilatory response to hypercapnia. Determination of potential therapeutic targets for the treatment of central alveolar hypoventilation syndromes.” 2018. Doctoral Dissertation, Sorbonne Paris Cité. Accessed January 24, 2021. http://www.theses.fr/2018USPCD019.

MLA Handbook (7^th Edition):

Perrin-Terrin, Anne-Sophie. “Étude de la modulation par l’étonogestrel, l’érythropoïétine et la leptine de la réponse ventilatoire à l'hypercapnie. Détermination de cibles thérapeutiques potentielles pour le traitement des syndromes d’hypoventilation alvéolaire centrale : Study of the modulation by etonogestrel, erythropoietin and leptin of the ventilatory response to hypercapnia. Determination of potential therapeutic targets for the treatment of central alveolar hypoventilation syndromes.” 2018. Web. 24 Jan 2021.

Vancouver:

Perrin-Terrin A. Étude de la modulation par l’étonogestrel, l’érythropoïétine et la leptine de la réponse ventilatoire à l'hypercapnie. Détermination de cibles thérapeutiques potentielles pour le traitement des syndromes d’hypoventilation alvéolaire centrale : Study of the modulation by etonogestrel, erythropoietin and leptin of the ventilatory response to hypercapnia. Determination of potential therapeutic targets for the treatment of central alveolar hypoventilation syndromes. [Internet] [Doctoral dissertation]. Sorbonne Paris Cité; 2018. [cited 2021 Jan 24]. Available from: http://www.theses.fr/2018USPCD019.

Council of Science Editors:

Perrin-Terrin A. Étude de la modulation par l’étonogestrel, l’érythropoïétine et la leptine de la réponse ventilatoire à l'hypercapnie. Détermination de cibles thérapeutiques potentielles pour le traitement des syndromes d’hypoventilation alvéolaire centrale : Study of the modulation by etonogestrel, erythropoietin and leptin of the ventilatory response to hypercapnia. Determination of potential therapeutic targets for the treatment of central alveolar hypoventilation syndromes. [Doctoral Dissertation]. Sorbonne Paris Cité; 2018. Available from: http://www.theses.fr/2018USPCD019

Universitat Politècnica de València

8. Morini, Marina Ange Marie. Molecular approaches related to the European eel (Anguilla anguilla) reproductive process .

Degree: 2016, Universitat Politècnica de València

URL: http://hdl.handle.net/10251/68513

► [EN] The European eel (Anguilla anguilla, L., 1758) population is in dramatic decline, so much so that this species has been listed as "Critically Endangered"… (more)

▼ [EN] The European eel (Anguilla anguilla, L., 1758) population is in dramatic decline, so much so that this species has been listed as "Critically Endangered" on the Red List of Threatened Species, by the International Union for Conservation of Nature (IUCN). The European eel has a complex life cycle, with sexual maturation blocked in the absence of the reproductive oceanic migration, and an inability to mature in captivity without the administration of hormonal treatments. Even though experimental maturation induces gamete production of both sexes, the fertilization results in infertile eggs, unviable embryos and larvae, which die within a few days of hatching. Therefore, understanding the eel reproductive physiology during maturation is very important if we want to recover the wild eel population. Furthermore, due to its phylogenetic position, representative of a basal group of teleosts, the Elopomorphs, the Anguilla species may provide insights into ancestral regulatory physiology processes of reproduction in teleosts, the largest group of vertebrates. In this thesis, characterization, phylogeny and synteny analyses have given us new insight into the evolutionary history of the reproductive process in vertebrates. The European eel possesses five membrane (mPRs) and two nuclear (nPR or pgrs) progestin receptors. Eel mPRs clustered in two major monophyletic groups. Phylogeny analysis of vertebrate nPRs and PLCz1 (sperm specific protein) places both eel PLCz1 and nPR sequences at the base of the teleost clade, which is consistent with the basal position of elopomorphs in the phylogeny of teleosts. To further resolve the origin of the duplicated eel nPRs, synteny analyses of the nPR neighboring genes in several vertebrate genomes were performed. Phylogeny and synteny analyses allowed us to propose the hypothesis that eel duplicated nPRs originated from the 3R. In order to gain a better understanding of the role of the genes implicated in eel reproduction, analyses of their regulation during experimental maturation were carried out. The change in salinity induced parallel increases in E2 plasma and nuclear estrogen receptor expression levels, revealing a stimulatory effect of salinity on the E2 signalling pathway along the BPG axis, leading to a control of spermatogonial stem cell renewal. Brain and pituitary estrogen receptors may then mediate the stimulation of androgens and steroidogenic enzymes linked to androgen synthesis. Androgen synthesis is not dependent on temperature, but further maturation requires higher temperatures to induce a change in the steroidogenic pathway towards estrogen and progestin synthesis. This is consistent with our studies on estrogen and progestin receptors. In the testis, progestin seems to regulate meiosis through membrane and nuclear progestin receptors, and final sperm maturation seems to be controlled by both estrogen and progestin through the estrogen and progestin membrane receptors. Finally, eel sperm-specific PLCz1 seems to have an important function in spermatozoa by inducing… Advisors/Committee Members: Asturiano Nemesio, Juan Francisco (advisor), Sánchez Peñaranda, David (advisor).

Subjects/Keywords: Receptor; estrogen; progestin; spermatogenesis; eel maturation; reproduction; enzyme; fecondation; phylogeny

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Morini, M. A. M. (2016). Molecular approaches related to the European eel (Anguilla anguilla) reproductive process . (Doctoral Dissertation). Universitat Politècnica de València. Retrieved from http://hdl.handle.net/10251/68513

Chicago Manual of Style (16^th Edition):

Morini, Marina Ange Marie. “Molecular approaches related to the European eel (Anguilla anguilla) reproductive process .” 2016. Doctoral Dissertation, Universitat Politècnica de València. Accessed January 24, 2021. http://hdl.handle.net/10251/68513.

MLA Handbook (7^th Edition):

Morini, Marina Ange Marie. “Molecular approaches related to the European eel (Anguilla anguilla) reproductive process .” 2016. Web. 24 Jan 2021.

Vancouver:

Morini MAM. Molecular approaches related to the European eel (Anguilla anguilla) reproductive process . [Internet] [Doctoral dissertation]. Universitat Politècnica de València; 2016. [cited 2021 Jan 24]. Available from: http://hdl.handle.net/10251/68513.

Council of Science Editors:

Morini MAM. Molecular approaches related to the European eel (Anguilla anguilla) reproductive process . [Doctoral Dissertation]. Universitat Politècnica de València; 2016. Available from: http://hdl.handle.net/10251/68513

Universiteit Utrecht

9. Chen, S.X. Function of progestin and its nuclear receptor in fish spermatogenesis.

Degree: 2010, Universiteit Utrecht

URL: http://dspace.library.uu.nl:8080/handle/1874/179788

► In this thesis, we set out to clone Pgrs from three fish species belonging to different orders, namely the zebrafish (Cypriniformes), the Atlantic salmon (Salmoniformes)… (more)

▼ In this thesis, we set out to clone Pgrs from three fish species belonging to different orders, namely the zebrafish (Cypriniformes), the Atlantic salmon (Salmoniformes) and the Atlantic cod (Gadiformes). Cloning of the full-length cDNAs was the prerequisite for expressing these receptors in a host cell line, which allowed functional characterization of the receptors. These studies indicated that 17alpha,20beta-dihydroxy-4-pregnen-3-one (DHP), a typical progestin in fish, is the most effective native ligand for the teleost Pgrs cloned in this thesis. Moreover, the cloned pgrs are predominantly expressed in testis. Taken together, these first results suggest that Pgrs have important function on the testicular level. Furthermore, the cellular localization of the pgr transcripts was investigated in testis using in situ hybridization. The results indicated pgr mRNA specific signals were observed in Sertoli cells from all three fish species. In zebrafish, pgr mRNA was also detected in Leydig cells. Using zebrafish, our results indicated that the role for Pgr expressed in Leydig cells is to modulate intratesticular levels of other steroid hormones (e.g. cortisol or 11-ketotestosterone) by increasing 11betaHsd activity. Using salmon, quantification of pgr mRNA in testis in relation to plasma DHP levels and germ cell development suggested that the salmon Pgr may be involved in the regulation of early spermatogenesis, in particular the differentiation of late type B spermatogonia and the entry into meiosis. In line with studies in Atlantic salmon, the quantification of pgr mRNA in cod testis during the onset of spermatogenesis indicated that the cod Pgr may be involved in the regulation of the late mitotic and meiotic phases of spermatogenesis. Moreover, the receptor was strongly up-regulated in cod testis tissue approaching the spawning condition, suggesting roles for the Pgr during the final stages of the reproductive cycle, such as has been described previously for other species (e.g. sperm hydration and spermatozoa mobility). Subsequently, using an in vivo experimental model (oestrogen-mediated down-regulation of androgen production to interrupt spermatogenesis), and a recently developed, ex vivo primary zebrafish testis tissue culture system, we could show that DHP treatment induced the proliferation of early spermatogonia as well as their differentiation into late spermatogonia and spermatocytes. Further, transcripts of Sertoli cell-derived growth factor genes were up-regulated after DHP treatment in vivo, but only insulin-like growth factor 1b receptor (igf1rb) mRNA levels showed a significant increase under DHP treatment in vivo and ex vivo. The present thesis expanded the knowledge on the roles of progestins and its nuclear receptor in fish spermatogenesis. Moreover, our results were taken to develop a model in which we suggest that the mitotic phase of spermatogenesis, under the overall support by gonadotropins, is driven by a potential steroid-Igf signalling axis, which may operate in concert with other paracrine… Advisors/Committee Members: Horst, D.J. van der, Schulz, R.W., Bogerd, J..

Subjects/Keywords: Biologie; zebrafish; atlantic salmon; atlantic cod; progestin; nuclear progesterone receptor; spermatogenesis; steroidogenesis

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Chen, S. X. (2010). Function of progestin and its nuclear receptor in fish spermatogenesis. (Doctoral Dissertation). Universiteit Utrecht. Retrieved from http://dspace.library.uu.nl:8080/handle/1874/179788

Chicago Manual of Style (16^th Edition):

Chen, S X. “Function of progestin and its nuclear receptor in fish spermatogenesis.” 2010. Doctoral Dissertation, Universiteit Utrecht. Accessed January 24, 2021. http://dspace.library.uu.nl:8080/handle/1874/179788.

MLA Handbook (7^th Edition):

Chen, S X. “Function of progestin and its nuclear receptor in fish spermatogenesis.” 2010. Web. 24 Jan 2021.

Vancouver:

Chen SX. Function of progestin and its nuclear receptor in fish spermatogenesis. [Internet] [Doctoral dissertation]. Universiteit Utrecht; 2010. [cited 2021 Jan 24]. Available from: http://dspace.library.uu.nl:8080/handle/1874/179788.

Council of Science Editors:

Chen SX. Function of progestin and its nuclear receptor in fish spermatogenesis. [Doctoral Dissertation]. Universiteit Utrecht; 2010. Available from: http://dspace.library.uu.nl:8080/handle/1874/179788

Virginia Tech

10. Utt, Matthew Douglas. The effects of varying the interval from follicular wave emergence to progestin withdrawal on follicular dynamics and the synchrony of estrus in beef cattle.

Degree: MS, Animal and Poultry Sciences, 2002, Virginia Tech

URL: http://hdl.handle.net/10919/33769

► The objective of this experiment was to examine the effects of varying the interval from follicular wave emergence to progestin removal on follicular dynamics and… (more)

Subjects/Keywords: GnRH; estrus synchronization; estradiol; follicle; progestin

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Utt, M. D. (2002). The effects of varying the interval from follicular wave emergence to progestin withdrawal on follicular dynamics and the synchrony of estrus in beef cattle. (Masters Thesis). Virginia Tech. Retrieved from http://hdl.handle.net/10919/33769

Chicago Manual of Style (16^th Edition):

Utt, Matthew Douglas. “The effects of varying the interval from follicular wave emergence to progestin withdrawal on follicular dynamics and the synchrony of estrus in beef cattle.” 2002. Masters Thesis, Virginia Tech. Accessed January 24, 2021. http://hdl.handle.net/10919/33769.

MLA Handbook (7^th Edition):

Utt, Matthew Douglas. “The effects of varying the interval from follicular wave emergence to progestin withdrawal on follicular dynamics and the synchrony of estrus in beef cattle.” 2002. Web. 24 Jan 2021.

Vancouver:

Utt MD. The effects of varying the interval from follicular wave emergence to progestin withdrawal on follicular dynamics and the synchrony of estrus in beef cattle. [Internet] [Masters thesis]. Virginia Tech; 2002. [cited 2021 Jan 24]. Available from: http://hdl.handle.net/10919/33769.

Council of Science Editors:

Utt MD. The effects of varying the interval from follicular wave emergence to progestin withdrawal on follicular dynamics and the synchrony of estrus in beef cattle. [Masters Thesis]. Virginia Tech; 2002. Available from: http://hdl.handle.net/10919/33769

University of Texas – Austin

11. Tan, Wenxian. Elucidating the signal cascades induced by progestins that mediate sperm hypermotility in Atlantic croaker (Micropogonias undulatus) and southern flounder (Paralichthys lethostigma).

Degree: PhD, Marine Science, 2013, University of Texas – Austin

URL: http://hdl.handle.net/2152/28716

► The overall goal of this research was to verify the involvement of membrane progestin receptor alpha (mPRα) in mediating progestin-stimulated sperm hypermotility in the Atlantic… (more)

▼ The overall goal of this research was to verify the involvement of membrane progestin receptor alpha (mPRα) in mediating progestin-stimulated sperm hypermotility in the Atlantic croaker and southern flounder. Sperm motility in Atlantic croaker and southern flounder were tested with both the endogenous progestin, 17,20β,21-trihydroxy-4-pregnen-3-one (20β-S) or the selective mPRα agonist, 10-ethenyl-19-norprogesterone (Org OD 02-0). In croaker, the Pi3k/Akt/Pde and ErbB2/Mapk intracellular signaling pathways were examined. The role of mPRα in mediating sperm hypermotility and fertility in southern flounder was also studied. The effects of seasonal hypoxia on sperm motility in croaker were investigated in a field study in the northern Gulf of Mexico in the fall of 2010. Finally, the effects of acidified activator solution (simulating ocean acidification) were studied in the laboratory. In vitro, Org OD 02-0 mimicked the stimulatory actions of 20β-S in inducing sperm hypermotility and intracellular signaling cascades in croaker and flounder sperm, indicating that mPRα is the mediator of progestin signaling in the sperm of these species. In croaker sperm, both the Pi3k/Akt/Pde and ErbB2/Mapk intracellular signaling pathways were shown to be important mediators of progestin-induced sperm hypermotility, suggesting novel functions of G [subscript olf] βγ-subunits in teleost sperm. In flounder sperm, mPRα was shown to be important in mediating sperm hypermotility as only high motility sperm with high expression of mPRα were responsive to progestin stimulation, resulting in higher fertilization success compared to low motility sperm. A single LHRHa injection resulted in increased sperm motility and fertility, associated with an increase in mPRα expression in the sperm plasma membrane. The results also suggest that the mPRα/Acy/cAMP pathway first described in croaker sperm is present in flounder sperm. Field studies of male Atlantic croaker exposed to chronic seasonal hypoxia showed that hypoxia exposure resulted in smaller gonads, lower spermatogenesis, reduced testicular mPRα expression, and in some sites, reduced sperm motility. Studies with croaker sperm using acidified activator solution to simulate ocean acidification indicated that croaker sperm were sensitive to environmental insult. Furthermore, the results suggested that the progestin signaling mechanism is more sensitive to changes in ocean pH levels than the mechanism that controls sperm motility. Advisors/Committee Members: Thomas, P. (Peter) (advisor).

Subjects/Keywords: Sperm; Hypermotility; Progestins; Fertility; Signaling; Membrane progestin receptors; Hypoxia; PH; Atlantic croaker; Southern flounder

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Tan, W. (2013). Elucidating the signal cascades induced by progestins that mediate sperm hypermotility in Atlantic croaker (Micropogonias undulatus) and southern flounder (Paralichthys lethostigma). (Doctoral Dissertation). University of Texas – Austin. Retrieved from http://hdl.handle.net/2152/28716

Chicago Manual of Style (16^th Edition):

Tan, Wenxian. “Elucidating the signal cascades induced by progestins that mediate sperm hypermotility in Atlantic croaker (Micropogonias undulatus) and southern flounder (Paralichthys lethostigma).” 2013. Doctoral Dissertation, University of Texas – Austin. Accessed January 24, 2021. http://hdl.handle.net/2152/28716.

MLA Handbook (7^th Edition):

Tan, Wenxian. “Elucidating the signal cascades induced by progestins that mediate sperm hypermotility in Atlantic croaker (Micropogonias undulatus) and southern flounder (Paralichthys lethostigma).” 2013. Web. 24 Jan 2021.

Vancouver:

Tan W. Elucidating the signal cascades induced by progestins that mediate sperm hypermotility in Atlantic croaker (Micropogonias undulatus) and southern flounder (Paralichthys lethostigma). [Internet] [Doctoral dissertation]. University of Texas – Austin; 2013. [cited 2021 Jan 24]. Available from: http://hdl.handle.net/2152/28716.

Council of Science Editors:

Tan W. Elucidating the signal cascades induced by progestins that mediate sperm hypermotility in Atlantic croaker (Micropogonias undulatus) and southern flounder (Paralichthys lethostigma). [Doctoral Dissertation]. University of Texas – Austin; 2013. Available from: http://hdl.handle.net/2152/28716

12. Garoche, Clémentine. Effets biologiques et mécanismes d'action de ligands environnementaux du récepteur nucléaire de la progestérone chez le poisson zèbre : Modes of action and biological effects of environmental ligands of the nuclear progesterone receptor in zebrafish.

Degree: Docteur es, Biologie, 2017, Rennes 1

URL: http://www.theses.fr/2017REN1B023

►

Chez les vertébrés, la progestérone (P4) est un progestatif endogène agissant via les récepteurs nucléaires et membranaires de la progestérone pour médier des processus clés… (more)

▼

Chez les vertébrés, la progestérone (P4) est un progestatif endogène agissant via les récepteurs nucléaires et membranaires de la progestérone pour médier des processus clés du développement et de la reproduction. De nombreuses molécules sont conçues pour mimer l’action de la P4. Ces progestatifs synthétiques et la P4 sont très utilisés en tant que substances pharmaceutiques et certains sont retrouvées dans l’environnement aquatique, posant un risque pour les organismes. Cependant, les informations sont insuffisantes pour évaluer les dangers et les risques de ces substances pour l’environnement. Dans ce travail de thèse, les mécanismes d’action et les effets biologiques des progestatifs sur le poisson zèbre ont été étudiés grâce à une combinaison d’outils in vitro et in vivo de type gène-rapporteur basés sur le mécanisme d’action des perturbateurs endocriniens. Nous avons caractérisé le profil toxicologique complexe de 26 progestatifs vis-à-vis de différents récepteurs nucléaires stéroïdiens humain et poisson zèbre et vis-à-vis de l’expression tissu-spécifique de gènes de la stéroïdogenèse (cyp19a1b dans le cerveau et cyp11c1 dans les interrénales). Nos résultats montrent que certains progestatifs peuvent perturber différentes voies de signalisation chez les larves en développement au niveau moléculaire, cellulaire et tissulaire ainsi qu’au niveau physiologique. Dans l’ensemble, les résultats de cette thèse permettent une meilleure caractérisation des dangers des progestatifs sur différentes cibles endocriniennes.

Among vertebrates, progesterone (P4) is an endogenous progestin acting through the nuclear and molecular progesterone receptor to mediate key processes of development and reproduction. Many molecules are designed to mimic the action of P4. These synthetic progestins and P4 are widely used as pharmaceuticals and some of them are found in this aquatic environment, thus posing risks to aquatic species. However, information is lacking to evaluate the dangers and risks of these substances for the environment. This thesis studied the mechanisms of action and the effects of progestins on zebrafish, using a combination of reporter-gene in vitro and in vivo tools based on the mechanism of action of endocrine disruptors. We characterized the toxicological profile of 26 progestins towards different human and zebrafish nuclear steroid receptors and towards the tissue-specific expression of steroidogenic genes (cyp19a1b in the brain and cyp11c1 in the interrenal cells). Our results show that some progestins can disrupt different signaling pathways in developing larvae at the molecular level, cellular and tissular level, and physiological level. Overall, the results of this thesis allow for a better characterization of the dangers of progestins on several endocrine targets.

Advisors/Committee Members: Kah, Olivier (thesis director), Brion, François (thesis director).

Subjects/Keywords: Poisson zèbre; Perturbateur endocrinien; Progestatif; Progesterone; Bioessai; Environnement aquatique; Zebrafish; Endocrine disruptor; Progestin; Progesterone; Bioassay; Aquatic environment

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Garoche, C. (2017). Effets biologiques et mécanismes d'action de ligands environnementaux du récepteur nucléaire de la progestérone chez le poisson zèbre : Modes of action and biological effects of environmental ligands of the nuclear progesterone receptor in zebrafish. (Doctoral Dissertation). Rennes 1. Retrieved from http://www.theses.fr/2017REN1B023

Chicago Manual of Style (16^th Edition):

Garoche, Clémentine. “Effets biologiques et mécanismes d'action de ligands environnementaux du récepteur nucléaire de la progestérone chez le poisson zèbre : Modes of action and biological effects of environmental ligands of the nuclear progesterone receptor in zebrafish.” 2017. Doctoral Dissertation, Rennes 1. Accessed January 24, 2021. http://www.theses.fr/2017REN1B023.

MLA Handbook (7^th Edition):

Garoche, Clémentine. “Effets biologiques et mécanismes d'action de ligands environnementaux du récepteur nucléaire de la progestérone chez le poisson zèbre : Modes of action and biological effects of environmental ligands of the nuclear progesterone receptor in zebrafish.” 2017. Web. 24 Jan 2021.

Vancouver:

Garoche C. Effets biologiques et mécanismes d'action de ligands environnementaux du récepteur nucléaire de la progestérone chez le poisson zèbre : Modes of action and biological effects of environmental ligands of the nuclear progesterone receptor in zebrafish. [Internet] [Doctoral dissertation]. Rennes 1; 2017. [cited 2021 Jan 24]. Available from: http://www.theses.fr/2017REN1B023.

Council of Science Editors:

Garoche C. Effets biologiques et mécanismes d'action de ligands environnementaux du récepteur nucléaire de la progestérone chez le poisson zèbre : Modes of action and biological effects of environmental ligands of the nuclear progesterone receptor in zebrafish. [Doctoral Dissertation]. Rennes 1; 2017. Available from: http://www.theses.fr/2017REN1B023

University of Southern California

13. Atrio, Jessica Maria. Clinical research in women's reproductive health and the human immunodeficiency virus.

Degree: MS, Clinical, Biomedical and Translational Investigations, 2015, University of Southern California

URL: http://digitallibrary.usc.edu/cdm/compoundobject/collection/p15799coll3/id/290139/rec/1386

► Objective: Pharmacokinetic interactions exist between combined oral contraceptives and protease inhibitors (PI). However, such information is lacking for progestin-only oral contraception. We sought to define… (more)

Subjects/Keywords: HIV; hormonal contraception; contraceptive; protease inhibitor; progestin only pills; norethindrone; pharmacokinetics; contraception; drug interaction; pregnancy prevention; antiretroviral; Norvir

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Atrio, J. M. (2015). Clinical research in women's reproductive health and the human immunodeficiency virus. (Masters Thesis). University of Southern California. Retrieved from http://digitallibrary.usc.edu/cdm/compoundobject/collection/p15799coll3/id/290139/rec/1386

Chicago Manual of Style (16^th Edition):

Atrio, Jessica Maria. “Clinical research in women's reproductive health and the human immunodeficiency virus.” 2015. Masters Thesis, University of Southern California. Accessed January 24, 2021. http://digitallibrary.usc.edu/cdm/compoundobject/collection/p15799coll3/id/290139/rec/1386.

MLA Handbook (7^th Edition):

Atrio, Jessica Maria. “Clinical research in women's reproductive health and the human immunodeficiency virus.” 2015. Web. 24 Jan 2021.

Vancouver:

Atrio JM. Clinical research in women's reproductive health and the human immunodeficiency virus. [Internet] [Masters thesis]. University of Southern California; 2015. [cited 2021 Jan 24]. Available from: http://digitallibrary.usc.edu/cdm/compoundobject/collection/p15799coll3/id/290139/rec/1386.

Council of Science Editors:

Atrio JM. Clinical research in women's reproductive health and the human immunodeficiency virus. [Masters Thesis]. University of Southern California; 2015. Available from: http://digitallibrary.usc.edu/cdm/compoundobject/collection/p15799coll3/id/290139/rec/1386

University of Southern California

14. Saxena, Tanmai. Steroid hormones, hormone-related genes, reproductive factors and breast cancer in the California Teachers Study.

Degree: PhD, Epidemiology, 2013, University of Southern California

URL: http://digitallibrary.usc.edu/cdm/compoundobject/collection/p15799coll127/id/643780/rec/6069

► The California Teachers Study (CTS) is a prospective cohort of 133,479 women. The cohort was initiated in the fall of 1995 and participants were all… (more)

▼ The California Teachers Study (CTS) is a prospective cohort of 133,479 women. The cohort was initiated in the fall of 1995 and participants were all current and former public school teachers or administrators that belonged to the California State Teachers Retirement System (STRS). Each member joined the cohort when she completed the study baseline questionnaire assessing information on known breast cancer risk factors in addition to background, dietary, physical, reproductive, personal health, and family health histories. Members meeting residency requirements and giving consent were followed and incident invasive breast cancer diagnoses were identified through annual linkages with the California Cancer Registry (CCR). ❧ This dissertation evaluates the role of postmenopausal hormone therapy as well as genetic variation in hormone-related genes for their respective and combined role in breast cancer risk in the CTS. ❧ Chapter 1, entitled “Menopausal hormone therapy and subsequent risk of specific invasive breast cancer subtypes in the California Teachers Study” is the study of use, recency, duration, and formulation of hormone therapy in its relation to breast cancer risk. An abstract was published in the conference proceedings and a poster was accepted as part of the 2009 American Association for Cancer Research (AACR) Annual Meeting, Denver, Colorado. The complete manuscript is published in Cancer Epidemiology, Biomarkers & Prevention (Cancer Epidemiol Biomarkers Prev. 2010 Sep;19(9):2366-78. Epub 2010 Aug 10.). ❧ Chapter 2, entitled “Hormone-related genes, body mass index, menopausal hormone therapy, and breast cancer risk in the California Teachers Study,” examines the risk of breast cancer associated with common variation in five candidate hormone binding genes. Additionally, it evaluates how variation in these genes interacts with body mass index and menopausal hormone therapy in determining risk of invasive breast cancer. This analysis was completed in a nested case-control study in the CTS. Overall as well as breast cancer subtype specific results are presented for single nucleotide polymorphisms (SNPs) in AR, ESR1, ESR2, PGR, and SHBG. The manuscript is currently under review by the CTS Steering Committee. ❧ Chapter 3, entitled “Genetic Variation in Hormone Metabolism Pathway Genes, Reproductive-Related Factors, and Breast Cancer Risk in the California Teachers Study,” is another analysis in the CTS nested case-control study. It analyses 452 SNPs in 29 candidate genes in the hormone metabolism pathway for an interaction with reproductive breast cancer risk factors. Age at menarche, parity, age at first full-term pregnancy and age at natural menopause are analyzed for interaction with these common SNPs separately for all invasive breast cancer as well as ER+/PR+ invasive breast tumors. Advisors/Committee Members: McKean-Cowdin, RobertaWu, Anna (Committee Chair), Haiman, Christopher A. (Committee Member), Press, Michael (Committee Member).

Subjects/Keywords: breast cancer; hormone receptor; hormone therapy; estrogen therapy; estrogen-progestin therapy; interaction; genetic interaction; hormone metabolism; reproductive factors; risk

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Saxena, T. (2013). Steroid hormones, hormone-related genes, reproductive factors and breast cancer in the California Teachers Study. (Doctoral Dissertation). University of Southern California. Retrieved from http://digitallibrary.usc.edu/cdm/compoundobject/collection/p15799coll127/id/643780/rec/6069

Chicago Manual of Style (16^th Edition):

Saxena, Tanmai. “Steroid hormones, hormone-related genes, reproductive factors and breast cancer in the California Teachers Study.” 2013. Doctoral Dissertation, University of Southern California. Accessed January 24, 2021. http://digitallibrary.usc.edu/cdm/compoundobject/collection/p15799coll127/id/643780/rec/6069.

MLA Handbook (7^th Edition):

Saxena, Tanmai. “Steroid hormones, hormone-related genes, reproductive factors and breast cancer in the California Teachers Study.” 2013. Web. 24 Jan 2021.

Vancouver:

Saxena T. Steroid hormones, hormone-related genes, reproductive factors and breast cancer in the California Teachers Study. [Internet] [Doctoral dissertation]. University of Southern California; 2013. [cited 2021 Jan 24]. Available from: http://digitallibrary.usc.edu/cdm/compoundobject/collection/p15799coll127/id/643780/rec/6069.

Council of Science Editors:

Saxena T. Steroid hormones, hormone-related genes, reproductive factors and breast cancer in the California Teachers Study. [Doctoral Dissertation]. University of Southern California; 2013. Available from: http://digitallibrary.usc.edu/cdm/compoundobject/collection/p15799coll127/id/643780/rec/6069

Tampere University

15. Ahola, Tytti. The Role of Membrane-Initiated Signalling in Progestin-Induced Growth Inhibition in Mammary Epithelial and Breast Cancer Cells .

Degree: Lääketieteen laitos - Medical School, 2004, Tampere University

URL: https://trepo.tuni.fi/handle/10024/67383

► Progesteronin synteettistä muotoa progestiinia käytetään laajalti rintasyövän hoidossa, osana hormonikorvaushoitoa ja hormonaalista ehkäisyä. Viimeaikaiset tutkimukset hormonikorvaushoidoista kuitenkin osoittavat, että progestiini lisää rintasyövän riskiä. Tämän johdosta… (more)

▼ Progesteronin synteettistä muotoa progestiinia käytetään laajalti rintasyövän hoidossa, osana hormonikorvaushoitoa ja hormonaalista ehkäisyä. Viimeaikaiset tutkimukset hormonikorvaushoidoista kuitenkin osoittavat, että progestiini lisää rintasyövän riskiä. Tämän johdosta tutkimukset, jotka selvittävät progestiinin tapaa vaikuttaa solujen kasvuun ovat ajankohtaisia. Tässä työssä tutkittiin progestiinin vaikutusmekanismia käyttäen mallina rintasyöpäsoluja. Havaitsisimme progestiinin säätelevän solukalvon pintareseptorin GPR30:en (G protein-coupled receptor) ilmentymistä lähetti-RNA tasolla. GPR30:en ilmentyminen esti sekä rintasyöpäsolujen että ei-pahanlaatuisten maitorauhassolujen jakautumista. Osoitimme käyttäen ns antisense tekniikkaa, että GPR30:en ilmentyminen oli kriittinen progestiinin kyvylle estää solujen kasvua. Kun GPR30:en lähetti-RNA taso väheni, myös progestiinin vaikutus kasvuun heikkeni. Tutkimuksessamme progestiini esti MAPK (mitogen-activated protein kinase) solun signaalinvälitysjärjestelmän aktivaatiota GPR30 reseptorin kautta. GPR30:lla havaitsimme myös olevan vaikutusta geenien luennan aktiivisuuteen ja tätä säätelevän molekyylin TIF2 cofactorin ilmentymisen määrään. Työ osoittaa yhteneväisen vaikutusmekanismin solun pinnan reseptorin GPR30:en ja solun tumareseptoriin vaikuttavan progestiinin kesken. Tulokset viittaavat siihen, että progestiinin vaikutus soluihin ainakin osittain välittyisi solun pintareseptorin GPR30:en kautta. Työ tuo esiin uuden mahdollisen vaikutusmekanismin, jolla steroidihormonit säätelevät solujen jakautumista. Löytämällä reseptoriin sitoutuva molekyyli voidaan varmistaa nyt saadut tulokset ja mahdollisesti tätä molekyyliä käyttäen estää solujen jakautumista maitorauhassoluissa välttäen progestiinin kasvua lisäävä vaikutus.; 1. Progestins and progesterone enhanced the expression of GPCR mRNA at 24-48 h in different breast cancer cell lines. Other steroid hormones did not upregulate GPCR30 mRNA expression, and the expression was abrogated by anti-progestin RU486 suggesting progestin specificity in the mRNA regulation. Although a number of progestin target genes have been described, the result presented provides an interesting new insight into progestin signalling, since GPCR30 gene has been suggested to be critical for estrogen-mediated MAPK activation. 2. Transient expression of GPCR30 inhibited proliferation of MCF-7 breast cancer cells, and GPCR30 antisense enhanced proliferation. In immortalized HME cells stable expression of GPCR30 inhibited proliferation. We have characterized GPCR30 as a growth inhibitory receptor for the first time. The results are consistent with the evidence that other family members of GPCR30 inhibit growth, particularly the rat homologue of GPCR30. 3. Progestin-induced growth effects: cyclin D1 down-regulation, G1 phase arrest and inhibition of cell proliferation were diminished in MCF-7 cells when GPR30 expression was down-regulated by the antisense, suggesting that GPCR30 is critical for a progestininduced growth effect. Additionally, the…

Subjects/Keywords: progestiini ; GPR30 ; rintasyöpäsolut ; progestin ; GPR30 ; breast cancer cells

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Ahola, T. (2004). The Role of Membrane-Initiated Signalling in Progestin-Induced Growth Inhibition in Mammary Epithelial and Breast Cancer Cells . (Doctoral Dissertation). Tampere University. Retrieved from https://trepo.tuni.fi/handle/10024/67383

Chicago Manual of Style (16^th Edition):

Ahola, Tytti. “The Role of Membrane-Initiated Signalling in Progestin-Induced Growth Inhibition in Mammary Epithelial and Breast Cancer Cells .” 2004. Doctoral Dissertation, Tampere University. Accessed January 24, 2021. https://trepo.tuni.fi/handle/10024/67383.

MLA Handbook (7^th Edition):

Ahola, Tytti. “The Role of Membrane-Initiated Signalling in Progestin-Induced Growth Inhibition in Mammary Epithelial and Breast Cancer Cells .” 2004. Web. 24 Jan 2021.

Vancouver:

Ahola T. The Role of Membrane-Initiated Signalling in Progestin-Induced Growth Inhibition in Mammary Epithelial and Breast Cancer Cells . [Internet] [Doctoral dissertation]. Tampere University; 2004. [cited 2021 Jan 24]. Available from: https://trepo.tuni.fi/handle/10024/67383.

Council of Science Editors:

Ahola T. The Role of Membrane-Initiated Signalling in Progestin-Induced Growth Inhibition in Mammary Epithelial and Breast Cancer Cells . [Doctoral Dissertation]. Tampere University; 2004. Available from: https://trepo.tuni.fi/handle/10024/67383

16. Jimenez Filho, Diego Lobon [UNESP]. Fatores de risco para a saúde coletiva e para o meio ambiente na utilização de hormônios em programas de reprodução assistida em bovinos.

Degree: 2016, Universidade Estadual Paulista

URL: http://hdl.handle.net/11449/144965

► O objetivo geral do estudo foi avaliar os riscos sanitários associados ao uso de fontes exógenas de hormônios sexuais nos programas de sincronização e indução… (more)

▼ O objetivo geral do estudo foi avaliar os riscos sanitários associados ao uso de fontes exógenas de hormônios sexuais nos programas de sincronização e indução de ovulação “Inseminação Artificial em Tempo Fixo (IATF)”, “Transferência de Embriões em Tempo Fixo (TETF)”, "Superestimulação ovariana (SOV)” sobre a saúde coletiva e meio ambiente. Para isto, o trabalho foi dividido em quatro capítulos. O primeiro capítulo apresenta as considerações gerais sobre legislação, período de carência, equipamentos de proteção individual, grupos de risco e resíduos sólidos de saúde. No segundo capítulo o objetivo foi avaliar o conhecimento e a percepção de risco dos criadores de bovinos e médicos veterinários na utilização de hormônios e determinar os possíveis riscos para a saúde coletiva. Neste experimento foram entrevistados 65 criadores e 40 médicos veterinários que utilizavam hormônios nos programas de reprodução assistida em bovinos (IATF, TETF, SOV). A análise de correspondência múltipla foi processada com os fatores socioeconômicos (escolaridade, tempo na atividade) e a percepção de risco no uso de hormônios reprodutivos. A totalidade dos criadores entrevistados afirmaram que “os estabelecimentos que comercializam produtos veterinários não exigiram prescrição veterinária para a venda dos hormônios”. Os “períodos de carências” na carne e no leite eram desconhecidos por 69,2% dos criadores e por 65% dos médicos veterinários. Dos profissionais que afirmaram conhecer a informação, apenas dois a citaram corretamente. O “uso de equipamentos de proteção individual (EPI)” durante a manipulação dos hormônios foi declarado por 56,9% dos criadores e 92,5% dos médicos veterinários, sendo “as luvas de procedimentos” o EPI mais utilizado. Ao serem questionados sobre “o grupo de pessoas inaptas à manipulação hormonal”, 21,5% dos criadores e 62,5% dos médicos veterinários afirmaram conhecer o grupo de risco. Houve associação entre criadores com “ensino superior” e “tempo na atividade menor que 5 anos” com a variável “conhece o grupo de pessoas que não deve manipular hormônios” (p < 0,01). O fator socioeconômico dos médicos veterinários “mais de 11 anos na atividade” também apresentou associação com a variável “conhece o período de carência” (p < 0,05). O terceiro capítulo teve como objetivo avaliar o conhecimento dos criadores e médicos veterinários sobre o descarte dos resíduos sólidos de saúde (RSS) e avaliar a possível contaminação ambiental durante a lavagem dos dispositivos intravaginais de progesterona. Foram consideradas questões socioeconômicas e sobre o descarte dos RSS. Além das entrevistas, foram realizadas análises de progestinas na água utilizada na lavagem dos dispositivos intravaginais de progesterona. As progestinas foram extraídas por cromatografia líquida de alta eficiência (HPLC) e analisadas por espectrometria de massas sequencial (MS/MS). Dos 65 criadores, 18,5% disseram ter “recebido orientação de como promover o descarte dos RSS”, entretanto, apenas um criador entrevistado promove o “correto descarte desse material”. A… Advisors/Committee Members: Dutra, Iveraldo dos Santos [UNESP], Mingoti, Gisele Zoccal [UNESP], Universidade Estadual Paulista (UNESP).

Subjects/Keywords: Breeding biotechnology; Personal protective equipment; Withholding period; Progestin; Health solid waste; Biotécnicas reprodutivas; Equipamentos de proteção individual; Períodos de carência; Progestinas; Resíduos sólidos de saúde

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Jimenez Filho, D. L. [. (2016). Fatores de risco para a saúde coletiva e para o meio ambiente na utilização de hormônios em programas de reprodução assistida em bovinos. (Thesis). Universidade Estadual Paulista. Retrieved from http://hdl.handle.net/11449/144965

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Jimenez Filho, Diego Lobon [UNESP]. “Fatores de risco para a saúde coletiva e para o meio ambiente na utilização de hormônios em programas de reprodução assistida em bovinos.” 2016. Thesis, Universidade Estadual Paulista. Accessed January 24, 2021. http://hdl.handle.net/11449/144965.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Jimenez Filho, Diego Lobon [UNESP]. “Fatores de risco para a saúde coletiva e para o meio ambiente na utilização de hormônios em programas de reprodução assistida em bovinos.” 2016. Web. 24 Jan 2021.

Vancouver:

Jimenez Filho DL[. Fatores de risco para a saúde coletiva e para o meio ambiente na utilização de hormônios em programas de reprodução assistida em bovinos. [Internet] [Thesis]. Universidade Estadual Paulista; 2016. [cited 2021 Jan 24]. Available from: http://hdl.handle.net/11449/144965.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Jimenez Filho DL[. Fatores de risco para a saúde coletiva e para o meio ambiente na utilização de hormônios em programas de reprodução assistida em bovinos. [Thesis]. Universidade Estadual Paulista; 2016. Available from: http://hdl.handle.net/11449/144965

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

17. Campion, Caroline. Progesterone Receptor Isoform Signaling In Ovarian Cancer Cellular Senescence.

Degree: PhD, Microbiology, Immunology and Cancer Biology, 2015, University of Minnesota

URL: http://hdl.handle.net/11299/175339

► As the deadliest of all gynecologic malignancies, ovarian cancer has a death rate of more than 50% due to late detection and diagnosis of the… (more)

▼ As the deadliest of all gynecologic malignancies, ovarian cancer has a death rate of more than 50% due to late detection and diagnosis of the disease and intrinsic or acquired resistance to current therapeutic regimens. The identification of robust biomarkers for early detection will have a substantial impact on survival rates, while prognostic molecular markers may allow for efficacious targeted therapeutic strategies. Progesterone plays a pivotal role in the development and progression of hormone-regulated tumors. In the breast, progesterone promotes a proliferative, pro-survival response, but inhibits growth in the uterus and ovary. The opposing biology in ovarian versus breast cancer cells may be largely dependent on cell context. The paradoxical effects of progesterone observed in ovarian relative to breast cancer cells may be attributed to actions of the nuclear progesterone receptor (PR) and its isoforms, PR-B and PR-A and their relative regulation (i.e. by post-translational modifications and co-factor binding partners), differential cross-talk between PR and growth factor-mediated signaling pathways (i.e. protein kinases), and/or altered expression levels in the target cells. In contrast to breast cancer, the detailed mechanisms of progesterone action in ovarian cancer are poorly understood. The goals of our studies were to determine the level of PR isoform expression in primary and cell models of ovarian cancer, to define the biological consequences of PR expression and activity on ovarian cancer cell biology, and to identify the key cofactor(s) required for mediating PR-dependent signaling actions. We demonstrated that ligand-activated PR-B induced a non-proliferative cell fate, known as cellular senescence, through a FOXO1-dependent mechanism in ovarian cancer cells. PR-B and FOXO1 were co-recruited to the same PRE-containing region of the upstream promoter of p21 upon progestin (R5020) treatment. Both proteins are required to cooperatively activate p21 expression based on data from PR-null control cells and lentiviral-delivered shRNA FOXO1-knockdown studies. Stable knock-down of FOXO1 inhibited progestin-induced p21 expression in ES-2 cells stably expressing GFP-tagged PR-B and blocked PR-dependent cellular senescence. Next, we investigated the biological consequences of PR isoform-specific gene regulation in ovarian cancer models, as well as directly compared PR isoform-selective transcriptomes between ovarian and breast cancer models. In ovarian cancer cells, PR-A is relatively insensitive to hormone, and PR-B (but not PR-A) is capable of inducing FOXO1 and p21 expression required for progestin-mediated cellular senescence in ovarian cancer cells. Furthermore, our studies revealed FOXO1 as a critical cofactor and determinant for the regulation of PR hormone sensitivity. Namely, activated FOXO1 confers PR-B-like behavior to PR-A, and in the presence of FOXO1, PR-A trans-activates classical PR-B target genes and induces robust cellular senescence. Finally, we utilized a novel ex vivo explant…

Subjects/Keywords: Breast Cancer; FOXO1; Ovarian Cancer; Progesterone Receptor; progestin; Senescence

…34 Figure 2-8: Progestin treatment of GFP-PR-containing cells stimulates FOXO1 expression… …in ovarian and breast cancer cells. �� 70 Figure 3-6: Progestin treatment… …10: FOXO1 enhances progestin-dependent PR-A transcriptional activity in Hela cells… …85 Figure 3-12: Progestin mediates p21 and FOXO1 expression ex vivo in PR+ human primary… …use of progestin-containing oral contraceptives is associated with decreasing lifetime risk…

6 more images…

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Campion, C. (2015). Progesterone Receptor Isoform Signaling In Ovarian Cancer Cellular Senescence. (Doctoral Dissertation). University of Minnesota. Retrieved from http://hdl.handle.net/11299/175339

Chicago Manual of Style (16^th Edition):

Campion, Caroline. “Progesterone Receptor Isoform Signaling In Ovarian Cancer Cellular Senescence.” 2015. Doctoral Dissertation, University of Minnesota. Accessed January 24, 2021. http://hdl.handle.net/11299/175339.

MLA Handbook (7^th Edition):

Campion, Caroline. “Progesterone Receptor Isoform Signaling In Ovarian Cancer Cellular Senescence.” 2015. Web. 24 Jan 2021.

Vancouver:

Campion C. Progesterone Receptor Isoform Signaling In Ovarian Cancer Cellular Senescence. [Internet] [Doctoral dissertation]. University of Minnesota; 2015. [cited 2021 Jan 24]. Available from: http://hdl.handle.net/11299/175339.

Council of Science Editors:

Campion C. Progesterone Receptor Isoform Signaling In Ovarian Cancer Cellular Senescence. [Doctoral Dissertation]. University of Minnesota; 2015. Available from: http://hdl.handle.net/11299/175339

University of Maryland

18. Bauer, Rosemary Aileen. Priming with Oral Progestin Before Ovulation Induction Facilitates Ovarian Function in the Cat (Felis catus).

Degree: Animal Sciences, 2007, University of Maryland

URL: http://hdl.handle.net/1903/7667

► Artificial insemination (AI) has been developed in multiple felid species as a tool for retaining gene diversity in threatened or endangered populations. Yet, pregnancy success… (more)

▼ Artificial insemination (AI) has been developed in multiple felid species as a tool for retaining gene diversity in threatened or endangered populations. Yet, pregnancy success remains low (< 5%) following AI in most felids, particularly in species that spontaneously ovulate. This failure has been attributed to variable ovarian status at the time of insemination and adverse residual effects caused by exogenous gonadotropins used to induce ovulation. Using the domestic cat as a research model, a new AI regimen that incorporated short-term ovarian suppression with oral progestin (altrenogest; ALT) before ovulation induction was investigated. The hypothesis was that oral progestin priming would prevent spontaneous ovulation, improve ovarian responsiveness to exogenous gonadotropins and mitigate adverse effects caused by persistent gonadotropin actions. Specific objectives were to: (1) increase fundamental understanding of the mechanisms controlling ovarian function; and (2) characterize how oral progestin priming prior to exogenous gonadotropin treatment influences ovarian responsiveness, fertilization, early embryonic development and luteal function in the cat. Fecal hormone monitoring was used to establish an ALT dosage that provides rapid, reversible ovarian suppression with no residual effects on estrous cyclicity. With this information, the influence of progestin priming on ovarian responsiveness to exogenous gonadotropin dosage was investigated. Priming increased ovarian sensitivity to gonadotropins, supporting the use of lower dosages for ovulation induction. Next, in vivo fertilization success and in vitro early embryonic development was characterized following laparoscopic, intrauterine AI in cats treated with ALT. Progestin-primed females demonstrated a good ovarian response to ovulation induction and more consistent embryonic development, compared to cats treated with gonadotropins alone. Furthermore, endocrine data revealed that normal luteal progesterone levels were maintained only in queens primed with the oral progestin. Finally, histology and quantitative RT-PCR were used to characterize the differential effects on luteal function observed. Aberrant CL progesterone production was not associated with changes in ovarian morphology, or the expression of six specific genes associated with luteal function and progesterone biosynthesis. Overall, these studies increased knowledge of domestic cat reproductive physiology and improved understanding of ovarian suppression for enhanced AI efficiency in felids. Advisors/Committee Members: Ottinger, Mary Ann (advisor).

Subjects/Keywords: Biology, Animal Physiology; cat; ovary; progestin; gonadotropin; endocrine; fecal steroids

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Bauer, R. A. (2007). Priming with Oral Progestin Before Ovulation Induction Facilitates Ovarian Function in the Cat (Felis catus). (Thesis). University of Maryland. Retrieved from http://hdl.handle.net/1903/7667

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Bauer, Rosemary Aileen. “Priming with Oral Progestin Before Ovulation Induction Facilitates Ovarian Function in the Cat (Felis catus).” 2007. Thesis, University of Maryland. Accessed January 24, 2021. http://hdl.handle.net/1903/7667.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Bauer, Rosemary Aileen. “Priming with Oral Progestin Before Ovulation Induction Facilitates Ovarian Function in the Cat (Felis catus).” 2007. Web. 24 Jan 2021.

Vancouver:

Bauer RA. Priming with Oral Progestin Before Ovulation Induction Facilitates Ovarian Function in the Cat (Felis catus). [Internet] [Thesis]. University of Maryland; 2007. [cited 2021 Jan 24]. Available from: http://hdl.handle.net/1903/7667.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Bauer RA. Priming with Oral Progestin Before Ovulation Induction Facilitates Ovarian Function in the Cat (Felis catus). [Thesis]. University of Maryland; 2007. Available from: http://hdl.handle.net/1903/7667

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Georgia

19. McLean, Amy Katherine. Using controlled internal drug release (CIDR) inserts for estrus synchronization in dairy heifers.

Degree: 2014, University of Georgia

URL: http://hdl.handle.net/10724/21336

► In Experiment 1, 164 heifers were assigned to two treatment groups. Each received a CIDR insert for 7 days. Treatment 1 received prostaglandin (PGF2 á… (more)

Subjects/Keywords: Controlled Internal Drug Release (CIDR); Dairy heifer; Estrus synchronization; Progestin; Estradiol cypionate (ECP); Gonadotropin releasing hormone (GnRH); Prostaglandin (PGF2á); and Timed artificial insemination (TAI)

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

McLean, A. K. (2014). Using controlled internal drug release (CIDR) inserts for estrus synchronization in dairy heifers. (Thesis). University of Georgia. Retrieved from http://hdl.handle.net/10724/21336

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

McLean, Amy Katherine. “Using controlled internal drug release (CIDR) inserts for estrus synchronization in dairy heifers.” 2014. Thesis, University of Georgia. Accessed January 24, 2021. http://hdl.handle.net/10724/21336.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

McLean, Amy Katherine. “Using controlled internal drug release (CIDR) inserts for estrus synchronization in dairy heifers.” 2014. Web. 24 Jan 2021.

Vancouver:

McLean AK. Using controlled internal drug release (CIDR) inserts for estrus synchronization in dairy heifers. [Internet] [Thesis]. University of Georgia; 2014. [cited 2021 Jan 24]. Available from: http://hdl.handle.net/10724/21336.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

McLean AK. Using controlled internal drug release (CIDR) inserts for estrus synchronization in dairy heifers. [Thesis]. University of Georgia; 2014. Available from: http://hdl.handle.net/10724/21336

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

20. Chen, S.X. Function of progestin and its nuclear receptor in fish spermatogenesis.

Degree: 2010, University Utrecht

URL: https://dspace.library.uu.nl/handle/1874/179788 ; URN:NBN:NL:UI:10-1874-179788 ; 1874/179788 ; URN:NBN:NL:UI:10-1874-179788 ; https://dspace.library.uu.nl/handle/1874/179788

► In this thesis, we set out to clone Pgrs from three fish species belonging to different orders, namely the zebrafish (Cypriniformes), the Atlantic salmon (Salmoniformes)… (more)

▼ In this thesis, we set out to clone Pgrs from three fish species belonging to different orders, namely the zebrafish (Cypriniformes), the Atlantic salmon (Salmoniformes) and the Atlantic cod (Gadiformes). Cloning of the full-length cDNAs was the prerequisite for expressing these receptors in a host cell line, which allowed functional characterization of the receptors. These studies indicated that 17alpha,20beta-dihydroxy-4-pregnen-3-one (DHP), a typical progestin in fish, is the most effective native ligand for the teleost Pgrs cloned in this thesis. Moreover, the cloned pgrs are predominantly expressed in testis. Taken together, these first results suggest that Pgrs have important function on the testicular level. Furthermore, the cellular localization of the pgr transcripts was investigated in testis using in situ hybridization. The results indicated pgr mRNA specific signals were observed in Sertoli cells from all three fish species. In zebrafish, pgr mRNA was also detected in Leydig cells. Using zebrafish, our results indicated that the role for Pgr expressed in Leydig cells is to modulate intratesticular levels of other steroid hormones (e.g. cortisol or 11-ketotestosterone) by increasing 11betaHsd activity. Using salmon, quantification of pgr mRNA in testis in relation to plasma DHP levels and germ cell development suggested that the salmon Pgr may be involved in the regulation of early spermatogenesis, in particular the differentiation of late type B spermatogonia and the entry into meiosis. In line with studies in Atlantic salmon, the quantification of pgr mRNA in cod testis during the onset of spermatogenesis indicated that the cod Pgr may be involved in the regulation of the late mitotic and meiotic phases of spermatogenesis. Moreover, the receptor was strongly up-regulated in cod testis tissue approaching the spawning condition, suggesting roles for the Pgr during the final stages of the reproductive cycle, such as has been described previously for other species (e.g. sperm hydration and spermatozoa mobility). Subsequently, using an in vivo experimental model (oestrogen-mediated down-regulation of androgen production to interrupt spermatogenesis), and a recently developed, ex vivo primary zebrafish testis tissue culture system, we could show that DHP treatment induced the proliferation of early spermatogonia as well as their differentiation into late spermatogonia and spermatocytes. Further, transcripts of Sertoli cell-derived growth factor genes were up-regulated after DHP treatment in vivo, but only insulin-like growth factor 1b receptor (igf1rb) mRNA levels showed a significant increase under DHP treatment in vivo and ex vivo. The present thesis expanded the knowledge on the roles of progestins and its nuclear receptor in fish spermatogenesis. Moreover, our results were taken to develop a model in which we suggest that the mitotic phase of spermatogenesis, under the overall support by gonadotropins, is driven by a potential steroid-Igf signalling axis, which may operate in concert with other paracrine… Advisors/Committee Members: Horst, D.J. van der, Schulz, R.W., Bogerd, J..

Subjects/Keywords: zebrafish; atlantic salmon; atlantic cod; progestin; nuclear progesterone receptor; spermatogenesis; steroidogenesis

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Chen, S. X. (2010). Function of progestin and its nuclear receptor in fish spermatogenesis. (Doctoral Dissertation). University Utrecht. Retrieved from https://dspace.library.uu.nl/handle/1874/179788 ; URN:NBN:NL:UI:10-1874-179788 ; 1874/179788 ; URN:NBN:NL:UI:10-1874-179788 ; https://dspace.library.uu.nl/handle/1874/179788

Chicago Manual of Style (16^th Edition):

Chen, S X. “Function of progestin and its nuclear receptor in fish spermatogenesis.” 2010. Doctoral Dissertation, University Utrecht. Accessed January 24, 2021. https://dspace.library.uu.nl/handle/1874/179788 ; URN:NBN:NL:UI:10-1874-179788 ; 1874/179788 ; URN:NBN:NL:UI:10-1874-179788 ; https://dspace.library.uu.nl/handle/1874/179788.

MLA Handbook (7^th Edition):

Chen, S X. “Function of progestin and its nuclear receptor in fish spermatogenesis.” 2010. Web. 24 Jan 2021.

Vancouver:

Chen SX. Function of progestin and its nuclear receptor in fish spermatogenesis. [Internet] [Doctoral dissertation]. University Utrecht; 2010. [cited 2021 Jan 24]. Available from: https://dspace.library.uu.nl/handle/1874/179788 ; URN:NBN:NL:UI:10-1874-179788 ; 1874/179788 ; URN:NBN:NL:UI:10-1874-179788 ; https://dspace.library.uu.nl/handle/1874/179788.

Council of Science Editors:

Chen SX. Function of progestin and its nuclear receptor in fish spermatogenesis. [Doctoral Dissertation]. University Utrecht; 2010. Available from: https://dspace.library.uu.nl/handle/1874/179788 ; URN:NBN:NL:UI:10-1874-179788 ; 1874/179788 ; URN:NBN:NL:UI:10-1874-179788 ; https://dspace.library.uu.nl/handle/1874/179788

University of Southern California

21. Razavi, Pedram. Hormonal and genetic risk factors of endometrial cancer and trends in incidence and survival of adult acute lymphoblastic leukemia.

Degree: PhD, Epidemiology, 2009, University of Southern California

URL: http://digitallibrary.usc.edu/cdm/compoundobject/collection/p15799coll127/id/278284/rec/3222

► This dissertation consists of three chapters:; The first chapter examines the association between lifetime postmenopausal hormone therapy (HT) use and risk of endometrial cancer using… (more)

Subjects/Keywords: acute lymphoblastic leukemia; race; ethnicity; hispanic; survival; incidence eate; body mass index; endometrial cancer; single nucleotide polymorphism; haplotype; postmenopausal hormone therapy; estrogen; progestin

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Razavi, P. (2009). Hormonal and genetic risk factors of endometrial cancer and trends in incidence and survival of adult acute lymphoblastic leukemia. (Doctoral Dissertation). University of Southern California. Retrieved from http://digitallibrary.usc.edu/cdm/compoundobject/collection/p15799coll127/id/278284/rec/3222

Chicago Manual of Style (16^th Edition):

Razavi, Pedram. “Hormonal and genetic risk factors of endometrial cancer and trends in incidence and survival of adult acute lymphoblastic leukemia.” 2009. Doctoral Dissertation, University of Southern California. Accessed January 24, 2021. http://digitallibrary.usc.edu/cdm/compoundobject/collection/p15799coll127/id/278284/rec/3222.

MLA Handbook (7^th Edition):

Razavi, Pedram. “Hormonal and genetic risk factors of endometrial cancer and trends in incidence and survival of adult acute lymphoblastic leukemia.” 2009. Web. 24 Jan 2021.

Vancouver:

Razavi P. Hormonal and genetic risk factors of endometrial cancer and trends in incidence and survival of adult acute lymphoblastic leukemia. [Internet] [Doctoral dissertation]. University of Southern California; 2009. [cited 2021 Jan 24]. Available from: http://digitallibrary.usc.edu/cdm/compoundobject/collection/p15799coll127/id/278284/rec/3222.

Council of Science Editors:

Razavi P. Hormonal and genetic risk factors of endometrial cancer and trends in incidence and survival of adult acute lymphoblastic leukemia. [Doctoral Dissertation]. University of Southern California; 2009. Available from: http://digitallibrary.usc.edu/cdm/compoundobject/collection/p15799coll127/id/278284/rec/3222

		in
And / Or
		in
And / Or
		in
And / Or
		in

Open Access Theses and Dissertations