subject:(pro inflammatory cytokine)

NSYSU

1. Mao, Pu-Wei. Suppressive Effects of Probiotics on Salmonella Induced Inflammation.

Degree: Master, Biological Sciences, 2016, NSYSU

URL: http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0729116-164844

► Salmonella infection in humans is commonly manifested as enterocolitis characterized by induction of epithelial secretion of pro-inflammatory cytokines and diarrhea, accompanied by infiltration of neutrophils… (more)

▼ Salmonella infection in humans is commonly manifested as enterocolitis characterized by induction of epithelial secretion of pro-inflammatory cytokines and diarrhea, accompanied by infiltration of neutrophils in the intestinal submucosa, which is a hall-mark of intestinal inflammation. The recruitment of neutrophils from circulation to the subepithelial region is facilitated by chemokines such as interleukin-8 (IL-8), which is known to be significantly induced upon bacterial entry by host epithelial cells. Probiotics are able to be used in the prevention or treatment of certain types of in-flammatory bowel disease (IBD). Certain types of Lactobacillus strains are successful in modulating pro-inflammatory cytokine signaling, which in turn, reduce inflammation in the gastrointestinal tract. In this study, we used the human intestinal cell line HCT 116 as an intestinal epithelial model, and the human leukemic monocyte cell line THP-1 as a leukocyte model, to determine the effects of four arbitrarily chosen strains of Lactobacillus probiotics on inflammation caused by Salmonella infection. The Salmonella strain used in the study is the wild-type SL1344, grown under anaerobic conditions to late log phase to maximize invasion phenotype. The four probiotic strains are Lactobacillus acidophilus, Lactoba-cillus rhamnosus, Lactobacillus paracasei, and Lactobacillus delbrueckii. Our results show that all four strains of probiotics were able to sporadically reduce mRNA expression of the three tested cytokines, but only Lactobacillus paracasei was able to consistently lower IL-8 expression. Our experimental results had shown in infection tests that upon Salmonella infection, the most acute response is seen in the upregu-lation of IL-8 expression, suggesting a possible relation between inflammation induced by bacterial infection and IL-8 induction. However, invasion assays show that Lactoba-cillus paracasei did not significantly reduce the amount of intracellular bacteria. In-stead, only Lactobacillus rhamnosus, which did not consistently suppress IL-8, signifi-cantly reduced bacterial invasion. These findings suggest that various probiotics sup-press inflammation through different mechanisms, and that the probiotic Lactobacillus rhamnosus is able to protect intestinal epithelial cells by rendering them less suscepti-ble to Salmonella invasion. Advisors/Committee Members: I-Fei Huang (chair), Chih-Wen Shu (chair), Chen Chun-Lin (committee member).

Subjects/Keywords: Salmonella; pro-inflammatory cytokine; inflammation; Lactobacillus; Probiotics; anti-inflammatory; intra-cellular pathogen

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Mao, P. (2016). Suppressive Effects of Probiotics on Salmonella Induced Inflammation. (Thesis). NSYSU. Retrieved from http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0729116-164844

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Mao, Pu-Wei. “Suppressive Effects of Probiotics on Salmonella Induced Inflammation.” 2016. Thesis, NSYSU. Accessed April 16, 2021. http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0729116-164844.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Mao, Pu-Wei. “Suppressive Effects of Probiotics on Salmonella Induced Inflammation.” 2016. Web. 16 Apr 2021.

Vancouver:

Mao P. Suppressive Effects of Probiotics on Salmonella Induced Inflammation. [Internet] [Thesis]. NSYSU; 2016. [cited 2021 Apr 16]. Available from: http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0729116-164844.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Mao P. Suppressive Effects of Probiotics on Salmonella Induced Inflammation. [Thesis]. NSYSU; 2016. Available from: http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0729116-164844

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Alberta

2. Ton, Kim Ph. Nutritional mitigation strategies for antibiotic free broiler production.

Degree: MS, Department of Agricultural, Food, and Nutritional Science, 2013, University of Alberta

URL: https://era.library.ualberta.ca/files/sn00b0209

► Nutritional strategies to mitigate the loss of broiler performance due to the elimination of prophylactic antibiotics were investigated, consisting of two nutrient density starter diets… (more)

Subjects/Keywords: innate immunity; 25 hydroxy vitamin D3; pro-inflammatory cytokine genes; ileal morphology; broiler

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Ton, K. P. (2013). Nutritional mitigation strategies for antibiotic free broiler production. (Masters Thesis). University of Alberta. Retrieved from https://era.library.ualberta.ca/files/sn00b0209

Chicago Manual of Style (16^th Edition):

Ton, Kim Ph. “Nutritional mitigation strategies for antibiotic free broiler production.” 2013. Masters Thesis, University of Alberta. Accessed April 16, 2021. https://era.library.ualberta.ca/files/sn00b0209.

MLA Handbook (7^th Edition):

Ton, Kim Ph. “Nutritional mitigation strategies for antibiotic free broiler production.” 2013. Web. 16 Apr 2021.

Vancouver:

Ton KP. Nutritional mitigation strategies for antibiotic free broiler production. [Internet] [Masters thesis]. University of Alberta; 2013. [cited 2021 Apr 16]. Available from: https://era.library.ualberta.ca/files/sn00b0209.

Council of Science Editors:

Ton KP. Nutritional mitigation strategies for antibiotic free broiler production. [Masters Thesis]. University of Alberta; 2013. Available from: https://era.library.ualberta.ca/files/sn00b0209

University of Queensland

3. Kazoullis, Andrea. Early interactions between Candida albicans yeasts and human cells in vitro.

Degree: School of Dentistry, 2020, University of Queensland

URL: https://espace.library.uq.edu.au/view/UQ:c664747/s4088646_final_thesis.pdf ; https://espace.library.uq.edu.au/view/UQ:c664747

► Candida species are ubiquitous opportunistic pathogens, causing significant morbidity and mortality in humans. C. albicans can asymptomatically colonise the skin and mucosal surfaces in healthy… (more)

▼ Candida species are ubiquitous opportunistic pathogens, causing significant morbidity and mortality in humans. C. albicans can asymptomatically colonise the skin and mucosal surfaces in healthy and immunologically competent individuals without causing disease. Impairment of normal host defence mechanisms can result in the conversion of C. albicans from a harmless commensal to an opportunistic pathogen that can initiate mucocutaneous forms of infections, such as oral candidiasis, vulvovaginal candidiasis (VVC), and even invasive systemic infections of the bloodstream and deep organs.In vitro and in vivo studies have revealed distinct patterns of strain-dependent tissue invasion and surface colonisation. Yeast strain differences markedly influence the nature and magnitude of both innate and adaptive immune responses against C. albicans infection. The following studies set out to delineate adherence properties and functional differences between two clinical isolates of C. albicans (one, 3630, cutaneous and one, 3683 oral). It was anticipated that the differences observed in mouse studies in vivo would be reflected in their interactions with human phagocytic cells in vitro.The oral isolate, 3683, was significantly more adherent than 3630 to human monocyte-derived macrophages (MDM), and epithelial cells, but not to mouse cells. A survey of six other oral isolates identified one that was like 3683 in its ability to adhere to both phagocytic and non-phagocytic human cells. The addition of homologous cell-free culture supernatant (SN) (termed the secretome) to yeast cells from the same isolate significantly increased adherence of 3630 compared to 3683. In contrast, the addition of secretome to yeast cells of 3683 did not affect adherence to epithelial cells.The secretome of C. albicans consists of glycoproteins that respond to the availability of nutrients and mediate evasion of host immune responses. Mass spectroscopy was performed on lyophilised secretome from both clinical isolates and identified several potential candidates that could be involved in inducing or modulating the host responses to these distinct clinical isolates.Isolate 3683 consistently induced significantly higher IL-1β production from THP-1 derived MDM and peripheral blood mononuclear cells (PBMC), compared to 3630. When the secretome of 3630 and 3683 was added to MDM, IL-1β production was greater from the MDM activated with 3630 SN than from 3683, demonstrating that the secretome from the two strains induced different patterns of responsiveness. The interactions between homologous and heterologous SN co-cultured with yeast cells showed that 3630 induced significantly more IL-1β in the presence of both SNs, whereas the converse was true for 3683. Heat treatment of the yeast cells and SN abrogated the release of IL-1β for both isolates. This pattern of secretion was also seen in the production of IL-18. The secretion of mature IL-1β and IL-18 resulting from caspase-1 cleavage of the two precursor molecules, confirmed assembly of the…

Subjects/Keywords: Innate immunity; Candida albicans; Adherence; Pro-inflammatory cytokine; Inflammasome; Pyroptosis; Secretome; 1105 Dentistry; 1107 Immunology

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Kazoullis, A. (2020). Early interactions between Candida albicans yeasts and human cells in vitro. (Thesis). University of Queensland. Retrieved from https://espace.library.uq.edu.au/view/UQ:c664747/s4088646_final_thesis.pdf ; https://espace.library.uq.edu.au/view/UQ:c664747

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Kazoullis, Andrea. “Early interactions between Candida albicans yeasts and human cells in vitro.” 2020. Thesis, University of Queensland. Accessed April 16, 2021. https://espace.library.uq.edu.au/view/UQ:c664747/s4088646_final_thesis.pdf ; https://espace.library.uq.edu.au/view/UQ:c664747.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Kazoullis, Andrea. “Early interactions between Candida albicans yeasts and human cells in vitro.” 2020. Web. 16 Apr 2021.

Vancouver:

Kazoullis A. Early interactions between Candida albicans yeasts and human cells in vitro. [Internet] [Thesis]. University of Queensland; 2020. [cited 2021 Apr 16]. Available from: https://espace.library.uq.edu.au/view/UQ:c664747/s4088646_final_thesis.pdf ; https://espace.library.uq.edu.au/view/UQ:c664747.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Kazoullis A. Early interactions between Candida albicans yeasts and human cells in vitro. [Thesis]. University of Queensland; 2020. Available from: https://espace.library.uq.edu.au/view/UQ:c664747/s4088646_final_thesis.pdf ; https://espace.library.uq.edu.au/view/UQ:c664747

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Freie Universität Berlin

4. Korten, Irma. Local immune response while a Helicobacter pylori infection in a vaccination study.

Degree: 2016, Freie Universität Berlin

URL: http://dx.doi.org/10.17169/refubium-7435

► Introduction: Infection with the bacterium Helicobacter pylori (H. pylori) is still one of the leading causes of gastritis and gastro-duodenal ulcer and predisposes to risk… (more)

▼ Introduction: Infection with the bacterium Helicobacter pylori (H. pylori) is still one of the leading causes of gastritis and gastro-duodenal ulcer and predisposes to risk for gastric cancer. Considering the increasing prevalence of resistance to antibiotics, the development of a reliable vaccine against H. pylori infections would constitute a major advance in the prevention of gastrointestinal illnesses. The objective of this work, which was carried out as part of a collaborative Helicobacter pylori vaccine study of the Max Planck Institute (MPI) for Infection Biology in Berlin and the Charité University Medicine Berlin, was to identify and characterize the role of forkhead box protein P3 (FOXP3)-expressing regulatory T lymphocytes (Treg) in the antral and duodenal mucosa in the pro-tection against H. pylori persistence and in the immunomodulation during infection. The work in-cludes the analysis of expression patterns of anti-inflammatory cytokines interleukin-4 (IL-4) and interleukin-10 (IL-10), of the immunomodulatory cytokine interferon-γ (INFγ), and of the pro-inflammatory cytokine tumor necrosis factor α (TNFα) during the course of an infection. Methods: 43 healthy H. pylori-negative participants were randomly assigned to three groups. Eleven participants received the H. pylori Urease A/B vaccine (DB2), another 11 participants received a vaccine with the H. pylori-spezific antigen HP231 (DB4), and a control group of 21 participants received the adjuvant only (Typhoral® L, an oral typhoid vaccine). All participants received a dosis of 105 bacteria of the H. pylori Baylor strain six weeks after vaccination. Histopathological examina-tions of the antral and duodenal mucosa biopsies were performed before and during the infection. Expression of FOXP3+ Treg in the antrum and duodenum was assessed with immunohistochemistry. Cytokine concentrations (INFγ, TNFα, IL-4 und IL-10) in the biopsy supernatants were measured via multiplex analyses using the Cytometric bead array (CBA), followed by flow cytometry. Results: Four of the 43 (9.3%) eradicated the infection (one participant from the DB4 group and three participants from the control group). There were no significant between- group differences regarding either FOXP3+ Treg or cytokine levels (INFγ, TNFα, IL-4 und IL-10) during the course of the infection. Participants successfully eradicating H. pylori exhibited, however, moderate alterations of cytokine and FOXP3 patterns. Conclusion: The DB2 and DB4 vaccines had no protective influence on H. pylori infection. The present results do not provide sufficient information with respect to a potential mechanism underly-ing H. pylori eradication. The study nevertheless provides further support for the hypothesis that successful eradication involves the Th1 immune response. Advisors/Committee Members: [email protected] (contact), w (gender), N.N. (firstReferee), N.N. (furtherReferee).

Subjects/Keywords: Helicobacter pylori infection; local immune response; FOXP3; anti-inflammatory and pro-inflammatory cytokine patterns; 600 Technik, Medizin, angewandte Wissenschaften::610 Medizin und Gesundheit::610 Medizin und Gesundheit

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Korten, I. (2016). Local immune response while a Helicobacter pylori infection in a vaccination study. (Thesis). Freie Universität Berlin. Retrieved from http://dx.doi.org/10.17169/refubium-7435

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Korten, Irma. “Local immune response while a Helicobacter pylori infection in a vaccination study.” 2016. Thesis, Freie Universität Berlin. Accessed April 16, 2021. http://dx.doi.org/10.17169/refubium-7435.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Korten, Irma. “Local immune response while a Helicobacter pylori infection in a vaccination study.” 2016. Web. 16 Apr 2021.

Vancouver:

Korten I. Local immune response while a Helicobacter pylori infection in a vaccination study. [Internet] [Thesis]. Freie Universität Berlin; 2016. [cited 2021 Apr 16]. Available from: http://dx.doi.org/10.17169/refubium-7435.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Korten I. Local immune response while a Helicobacter pylori infection in a vaccination study. [Thesis]. Freie Universität Berlin; 2016. Available from: http://dx.doi.org/10.17169/refubium-7435

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

5. Gonçalves , Barbara Hess Rodrigues. Correlação entre achados clínicos, histopatológicos e imunomarcação de interleucina 31 na pele de cães com dermatite atópica.

Degree: 2016, Universidade Federal de Goiás; Programa de Pós-graduação em Ciência Animal (EVZ); UFG; Brasil; Escola de Veterinária e Zootecnia – EVZ (RG)

URL: http://repositorio.bc.ufg.br/tede/handle/tede/6751

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Submitted by Luciana Ferreira ([email protected]) on 2017-01-17T11:14:30Z No. of bitstreams: 2 Dissertação - Barbara Hess Rodrigues Gonçalves - 2016.pdf: 1742093 bytes, checksum: 80bd9955d0b421c59b1e6faf6d41f773 (MD5) license_rdf: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5)

Approved for entry into archive by Luciana Ferreira ([email protected]) on 2017-01-17T11:14:56Z (GMT) No. of bitstreams: 2 Dissertação - Barbara Hess Rodrigues Gonçalves - 2016.pdf: 1742093 bytes, checksum: 80bd9955d0b421c59b1e6faf6d41f773 (MD5) license_rdf: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5)

Made available in DSpace on 2017-01-17T11:14:56Z (GMT). No. of bitstreams: 2 Dissertação - Barbara Hess Rodrigues Gonçalves - 2016.pdf: 1742093 bytes, checksum: 80bd9955d0b421c59b1e6faf6d41f773 (MD5) license_rdf: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5) Previous issue date: 2016-12-13

Conselho Nacional de Pesquisa e Desenvolvimento Científico e Tecnológico - CNPq

Canine atopic dermatitis (CAD) is the second most frequent disease in dermatological clinical routine of dogs. It is defined as a pruritic allergic skin disease, with genetic predisposition and clinical features, being related to the response to environmental allergens. Interleukin 31 (IL) 31 is a cytokine that participates in inflammatory processes and it is associated with pruritic diseases, especially those involving chronic inflammation such as allergic dermatitis. Produced by mononuclear cells, IL-31 is described to play an important role in the pathogenesis of atopic dermatitis. Two studies were performed in order to correlate clinical features, histopathological changes and the presence of IL-31 in the skin of dogs with atopic dermatitis. 34 dogs were selected from clinical routine, which 31 animals were diagnosed with CAD and three were healthy. The animals were evaluated for pruritus level by the owners' report and by clinical examination according to the CAD extent and severity index (CADESI-4). The dogs were grouped in discreetly, moderately and markedly compromised by CAD following the sum of scores’ values assigned in the clinical examination. Cutaneous samples from the axillary and interdigital regions of each dog were collected and submitted to histopathological (HE and toluidine blue) and immunohistochemical analyzes (IL-31). There was a correlation between the clinical score and the microscopic changes. As well, there was correlation among all the microscopic changes, but not between the degree of pruritus and the clinical score of CAD. Also, the inflammatory infiltrate was more intense in the axillary region in relation to interdigital skin. An increased numbers of cells immunostained for IL-31 was observed in dogs severely compromised by CAD. There was a correlation between the clinical score and the amount of interdigital mast cells, with an amount of cells immunostained for IL-31 in the axilla. It was also verified correlation between the amount of mast cells and cells immunostained for IL-31 in the axilla,…

Advisors/Committee Members: Moura , Veridiana Maria Brianezi Dignani de, Santin, Ana Paula Iglesias, Pôrto , Regiani Nascimento Gagno, Moura , Veridiana Maria Brianezi Dignani de, Farias , Marconi Rodrigues de, Lima, Aline Maria Vasconcelos.

Subjects/Keywords: Alergia canina; Citocina pró-inflamatória; Dermatites alérgicas; Dermatopatologia,; Infiltrado inflamatório crônico; Canine allergy; Allergic dermatitis; Pro-inflammatory cytokine; Dermatopathology; Chronic inflammatory infiltrate; CLINICA E CIRURGIA ANIMAL::CLINICA CIRURGICA ANIMAL

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Gonçalves , B. H. R. (2016). Correlação entre achados clínicos, histopatológicos e imunomarcação de interleucina 31 na pele de cães com dermatite atópica. (Masters Thesis). Universidade Federal de Goiás; Programa de Pós-graduação em Ciência Animal (EVZ); UFG; Brasil; Escola de Veterinária e Zootecnia – EVZ (RG). Retrieved from http://repositorio.bc.ufg.br/tede/handle/tede/6751

Chicago Manual of Style (16^th Edition):

Gonçalves , Barbara Hess Rodrigues. “Correlação entre achados clínicos, histopatológicos e imunomarcação de interleucina 31 na pele de cães com dermatite atópica.” 2016. Masters Thesis, Universidade Federal de Goiás; Programa de Pós-graduação em Ciência Animal (EVZ); UFG; Brasil; Escola de Veterinária e Zootecnia – EVZ (RG). Accessed April 16, 2021. http://repositorio.bc.ufg.br/tede/handle/tede/6751.

MLA Handbook (7^th Edition):

Gonçalves , Barbara Hess Rodrigues. “Correlação entre achados clínicos, histopatológicos e imunomarcação de interleucina 31 na pele de cães com dermatite atópica.” 2016. Web. 16 Apr 2021.

Vancouver:

Gonçalves BHR. Correlação entre achados clínicos, histopatológicos e imunomarcação de interleucina 31 na pele de cães com dermatite atópica. [Internet] [Masters thesis]. Universidade Federal de Goiás; Programa de Pós-graduação em Ciência Animal (EVZ); UFG; Brasil; Escola de Veterinária e Zootecnia – EVZ (RG); 2016. [cited 2021 Apr 16]. Available from: http://repositorio.bc.ufg.br/tede/handle/tede/6751.

Council of Science Editors:

Gonçalves BHR. Correlação entre achados clínicos, histopatológicos e imunomarcação de interleucina 31 na pele de cães com dermatite atópica. [Masters Thesis]. Universidade Federal de Goiás; Programa de Pós-graduação em Ciência Animal (EVZ); UFG; Brasil; Escola de Veterinária e Zootecnia – EVZ (RG); 2016. Available from: http://repositorio.bc.ufg.br/tede/handle/tede/6751

6. Bollenbach, Maud. Nouvelles cibles pour l'étude et le développement d'outils pharmacologiques originaux pour le traitement des douleurs neuropathiques : New targets for the study and development of new pharmacological tools for the treatment of neuropathic pain.

Degree: Docteur es, Chimie biologique et thérapeutique, 2017, Université de Strasbourg

URL: http://www.theses.fr/2017STRAF022

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Les douleurs neuropathiques désignent une hypersensibilité du système nerveux central sensoriel. C’est une maladie chronique et handicapante qui touche environ 6% de la population française.… (more)

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Les douleurs neuropathiques désignent une hypersensibilité du système nerveux central sensoriel. C’est une maladie chronique et handicapante qui touche environ 6% de la population française. Cependant, à l’heure actuelle, il n’existe pas de traitement spécifique et efficace. Dans le cadre de cette thèse, nous avons utilisé deux stratégies différentes afin de développer des outils pharmacologiques originaux pour traiter ces douleurs : une approche phénotypique autour de deux inhibiteurs de la surproduction de TNFα (un dérivé de 2-aminopyrimidine et un dérivé de pyridin-2-yl guanidine) et une approche moléculaire autour du MY 5445 (un inhibiteur de PDE5 dérivé de phtalazine). En particulier, notre travail s’est basé sur la conception, la synthèse et l’étude des relations structure-activité autour de ces différents hits et nous avons obtenu des composés efficaces par voie i.p. ou per-os sur un modèle murin de douleurs neuropathiques.En parallèle de ce travail de pharmacologie, nous avons développé différents systèmes catalytiques (Pd, Cu) en milieu micellaire afin de former des liaisons C-N à température quasi-ambiante.

Neuropathic pains correspond to a central sensory nervous system hypersensitivity. It is a chronic and disabling disease, which touch around 6% of the French population. Nowadays, there is no specific and efficient treatment. In my PhD project, we used two different strategies in order to develop innovative pharmacological tools to treat those pains: a phenotypic approach around two TNFα overproduction inhibitor (a 2-aminopyrimidine derivative and a pyridin-2-yl guanidine derivative) and a molecular approach around MY 5445 (a phthalazine PDE5 inhibitor). Our work was based on the design, synthesis and structure-activity relationship study around various hits and we obtained compounds i.p. and orally effective on a murin neuropathic pain model.In parallel to this pharmacological work, we developed different catalytic systems (Pd, Cu) under micellar conditions to form C-N bonds at almost room temperature.

Advisors/Committee Members: Schmitt, Martine (thesis director), Bihel, Frédéric (thesis director).

Subjects/Keywords: Douleur neuropathique; Cytokine pro-inflammatoire TNFα; Enzyme PDE5; Kinase MSK1; Relations structure-activité; Approche phénotypique; Approche moléculaire; Hétérocycles; Chimie verte; Réactions métallocatalysées; Neuropathic pain; Pro-inflammatory cytokine TNFα; PDE5 enzyme; MSK1 kinase; Structure-activity relationship; Phenotypic approach; Molecular approach; Heterocycles; Green chemistry; Metalocatalyzed reactions; 547.2; 615.19

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Bollenbach, M. (2017). Nouvelles cibles pour l'étude et le développement d'outils pharmacologiques originaux pour le traitement des douleurs neuropathiques : New targets for the study and development of new pharmacological tools for the treatment of neuropathic pain. (Doctoral Dissertation). Université de Strasbourg. Retrieved from http://www.theses.fr/2017STRAF022

Chicago Manual of Style (16^th Edition):

Bollenbach, Maud. “Nouvelles cibles pour l'étude et le développement d'outils pharmacologiques originaux pour le traitement des douleurs neuropathiques : New targets for the study and development of new pharmacological tools for the treatment of neuropathic pain.” 2017. Doctoral Dissertation, Université de Strasbourg. Accessed April 16, 2021. http://www.theses.fr/2017STRAF022.

MLA Handbook (7^th Edition):

Bollenbach, Maud. “Nouvelles cibles pour l'étude et le développement d'outils pharmacologiques originaux pour le traitement des douleurs neuropathiques : New targets for the study and development of new pharmacological tools for the treatment of neuropathic pain.” 2017. Web. 16 Apr 2021.

Vancouver:

Bollenbach M. Nouvelles cibles pour l'étude et le développement d'outils pharmacologiques originaux pour le traitement des douleurs neuropathiques : New targets for the study and development of new pharmacological tools for the treatment of neuropathic pain. [Internet] [Doctoral dissertation]. Université de Strasbourg; 2017. [cited 2021 Apr 16]. Available from: http://www.theses.fr/2017STRAF022.

Council of Science Editors:

Bollenbach M. Nouvelles cibles pour l'étude et le développement d'outils pharmacologiques originaux pour le traitement des douleurs neuropathiques : New targets for the study and development of new pharmacological tools for the treatment of neuropathic pain. [Doctoral Dissertation]. Université de Strasbourg; 2017. Available from: http://www.theses.fr/2017STRAF022

7. Celina Kassumi Kunieda Suzuki. AVALIAÇÃO DE POLIMORFISMOS GENÉTICOS DE SUSCEPTIBILIDADE À OBESIDADE ASSOCIADOS AO PERFIL MASTIGATÓRIO DE CRIANÇAS OBESAS.

Degree: 2012, Pontifícia Universidade Católica de Goiás

URL: http://tede.biblioteca.ucg.br/tde_busca/arquivo.php?codArquivo=1308

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The increased prevalence of childhood obesity in recent years suggests the existence of a genetic predisposition or susceptibility to conditioning factors for obesity which act… (more)

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The increased prevalence of childhood obesity in recent years suggests the existence of a genetic predisposition or susceptibility to conditioning factors for obesity which act on the environmental factors related to lifestyle involving diet and physical activity. Food education programs and physical activities have been developed in order to reverse this situation. The chewing is considered one of the early stages of the digestive process and therefore primordial important because performing correctly promotes natural hunger satiety as well as a healthy digestion. The aim of this study is to investigate the polymorphism of the TNF-α and DRD2 genes and the influence of masticatory profile in childhood obesity to enable the preparation of proposals for more effective interventions to control weight gain. For this purpose the study has been divided into two articles. ARTICLE 1: This study evaluated the profile of the obese child chewing through survey and evaluation of clinical history miofunctional from the protocols of MgBr and AMIOFE-A (attached) in 11 normal children representing the control population (Group I) and 16 obese children (Group B) participating in the CAIS Group of Obesity in Goiânia Municipal and School of Clinical Speech Therapy PUC Goiás aged between 6 and 13 years for both sexes. According to the findings obtained in the research, it is concluded that obese group (OB) presents an incision chewing food made with the central and lateral incisors with the majority labial sealing with or without excessive movement, unilateral pattern, with higher presence of altered behaviors in chewing and shorter chewing time. ARTICLE 2: This study evaluated the genetic polymorphism of TNF-α gene (G308A) and DRD2 (Taq1α - C32806T) in 27 patients with childhood obesity (16) and controls (11) using the method ARMS-PCR and PCR-RFLP, respectively, in peripheral blood samples. The results suggest that the genetic polymorphism of Taq1α (C32806T) in the DRD2 gene was associated with the increase of obesity in the childhood. Still genetic variants of the SNP G308A TNF-α gene is not possible to confirm its influence on childrens weight gain. Understanding how genetic variations affect the tendency to become or remain obese is an important step to comprehend the mechanisms that lead to obesity.

O aumento da prevalência da obesidade infantil nestes últimos anos sugere a existência de predisposição ou suscetibilidade genética como fatores condicionantes para a obesidade, sobre a qual atuam fatores ambientais relacionados ao estilo de vida, que envolvem hábitos alimentares e atividade física. Programas de educação alimentar e de atividades físicas foram desenvolvidos visando reverter este quadro. A mastigação é considerada uma das fases iniciais do processo digestório e de primordial importância, pois quando realizada de forma correta, promove a saciedade natural da fome, bem como uma digestão saudável. O objetivo deste estudo foi investigar o polimorfismo dos genes TNF-α e DRD2 e a influência da…

Advisors/Committee Members: Daniela de Melo e Silva, Cláudio Carlos da Silva, Cejana Baiocchi Souza.

Subjects/Keywords: Mastigação; Obesidade Infantil; Fonoaudiologia; Genética; Susceptibilidade; SNPs; Citocina pró-inflamatória; Dopamina; GENETICA; Mastication; Childhood Obesity; Speech Therapy; Genetics; Susceptibility, SNPs, pro-inflammatory cytokine; Dopamine

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Suzuki, C. K. K. (2012). AVALIAÇÃO DE POLIMORFISMOS GENÉTICOS DE SUSCEPTIBILIDADE À OBESIDADE ASSOCIADOS AO PERFIL MASTIGATÓRIO DE CRIANÇAS OBESAS. (Thesis). Pontifícia Universidade Católica de Goiás. Retrieved from http://tede.biblioteca.ucg.br/tde_busca/arquivo.php?codArquivo=1308

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Suzuki, Celina Kassumi Kunieda. “AVALIAÇÃO DE POLIMORFISMOS GENÉTICOS DE SUSCEPTIBILIDADE À OBESIDADE ASSOCIADOS AO PERFIL MASTIGATÓRIO DE CRIANÇAS OBESAS.” 2012. Thesis, Pontifícia Universidade Católica de Goiás. Accessed April 16, 2021. http://tede.biblioteca.ucg.br/tde_busca/arquivo.php?codArquivo=1308.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Suzuki, Celina Kassumi Kunieda. “AVALIAÇÃO DE POLIMORFISMOS GENÉTICOS DE SUSCEPTIBILIDADE À OBESIDADE ASSOCIADOS AO PERFIL MASTIGATÓRIO DE CRIANÇAS OBESAS.” 2012. Web. 16 Apr 2021.

Vancouver:

Suzuki CKK. AVALIAÇÃO DE POLIMORFISMOS GENÉTICOS DE SUSCEPTIBILIDADE À OBESIDADE ASSOCIADOS AO PERFIL MASTIGATÓRIO DE CRIANÇAS OBESAS. [Internet] [Thesis]. Pontifícia Universidade Católica de Goiás; 2012. [cited 2021 Apr 16]. Available from: http://tede.biblioteca.ucg.br/tde_busca/arquivo.php?codArquivo=1308.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Suzuki CKK. AVALIAÇÃO DE POLIMORFISMOS GENÉTICOS DE SUSCEPTIBILIDADE À OBESIDADE ASSOCIADOS AO PERFIL MASTIGATÓRIO DE CRIANÇAS OBESAS. [Thesis]. Pontifícia Universidade Católica de Goiás; 2012. Available from: http://tede.biblioteca.ucg.br/tde_busca/arquivo.php?codArquivo=1308

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

8. 박, 창은. Cell death and Pro-inflammatory Cytokine Release of Rat Microglial Cells by Naegleria fowleri.

Degree: 2008, Ajou University

URL: http://repository.ajou.ac.kr/handle/201003/1403 ; http://dcoll.ajou.ac.kr:9080/dcollection/jsp/common/DcLoOrgPer.jsp?sItemId=000000009136

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Naegleria fowleri, a free-living amoeba, causes fatal primary amoebic meningoencephalitis in humans and experimental animals. In previous study, N. fowleri trophozoites showed the highly cytopathic… (more)

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Naegleria fowleri, a free-living amoeba, causes fatal primary amoebic meningoencephalitis in humans and experimental animals. In previous study, N. fowleri trophozoites showed the highly cytopathic activity and cytotoxicity against rat microglial cells by the morphological observation and 51Cr release assay. In the present study, to determine whether a pathogenic N. fowleri lysate shows the cytopathic effects against primary cultured rat microglial cells, the morphological changes of microglial cells was observed by a light, scanning and transmission electron microscopes. And then, the cytotoxicity of N. fowleri lysate against rat microglial cells was also observed by (51)^Cr release assay. In addition, the pro-inflammatory cytokine release from microglial cells in co-culture system was estimated. As results with a light and electron microscopes, most of microglial cells were severely destroyed by N. fowleri lysate, showing the necrotic (above 85%) and apoptotic cell death (below 15%) in a time- and dose dependent manner. As the results of (51)^Cr release assay, the cytotoxicity of N. fowleri lysate against microglial cells were 14.6, 21.9, 38.5 and 71.5% at 3, 6, 12 and 24 h post incubation, respectively. And then, the amount of cytokines released from microglial cells in co-culture system at 3, 6 and 12 hr were 121.6, 90.4 and 81.0 pg/ml of TNF-α, 88.5, 92.7 and 190.1 pg/ml of IL-1β, and 298.8, 414.9 and 251.1 pg/ml of IL-6, respectively.

파울러자유아메바 (Naegleria fowleri)는 토양과 담수에서 자유생활을 하는 아메바로써 자연환경에서는 세균을 포식하며, 인체와 실험동물에서 원발성 아메바성 수막뇌염(priamry amoebic meningoencephalitis)을 일으키는 것으로 알려져 있다. 본 연구의 목적은 파울러 자유아메바의 전체세포 추출액(lysate)이 표적세포에 대한 아메바의 병원성과 관련성이 있는지를 입증하기 위해서, 일차적으로 배양한 쥐의 신경소교세포(microglial cells)와 함께 파울러자유아메바의 전체세포 추출액을 혼합 배양함으로써 신경소교세포들의 병변 효과를 광학, 주사, 및 투과 전자현미경을 이용해서 관찰하였다. 혼합 배양 3 시간 후, 소수의 신경소교세포들은 자유아메바들에 의해서 파괴가 되었으며, 배양 시간이 길어짐에 따라 신경소교세포들은 심하게 파괴되었다. 표적세포의 사멸과정을 DNA fragmentation 및 annexin V 염색법을 이용한 cell sorting(FACS)을 시행한 결과, 6시간 후 약 16% 정도가 세포사멸 (apoptosis) 과정을 통해 사멸하였다. 또한, 표적세포인 신경소교세포에 대한 아 메바 세포추출액의 세포독성을 생화학적 방법인 (51)^Cr 분비 실험으로 관찰한 결과, 신경소교세포들에 대한 자유아메바의 세포독성은 배양 후 3, 6, 12 그리고 24 시간에 각각 14.6, 21.9, 38.5 그리고 71.5% 이었다. 한편, 파울러자유아메바의 세포 추출액과 혼합 배양한 신경소교세포들은 방어적인 면역반응으로 다양한 싸이토카인들을 분비하였는데, TNF-α 및 IL-6등의 싸이토카인(cytokine) 분비량은 배양 초기부터 증가하였으며, IL-1β의 분비는 12시간 후부터 증가하였다.

"ABSTRACT = ⅰ TABLE OF CONTENTS = ⅲ LIST OF FIGURES = ⅴ LIST OF TABLES = ⅵ ABBREVIATION = ⅶ Ⅰ. INTRODUCTION = 1 A. NAEGLERIA FOWLERI = 1 1. Life cycle = 1 2. Incidence = 2 3. Symptoms = 2 4. Association with Water = 3 5. Pathogenesis = 4 B. Background = 5 C. Purpose = 8 Ⅱ. MATERIALS AND METHODS = 9 A. Amoeba and lysate = 9 B. Preparation of microglial cells = 9 C. Light microscopy = 10 D. Scanning electron microscopy = 10 E. Transmission electron microscopy = 11 F. DNA fragmentation = 11 G. Flow cytometry analysis = 12 H. In vitro cytotoxicity by chromium release assay = 13 I. ELISA for measurement of pro-inflammatory cytokines = 14 Ⅲ. RESULTS = 15 A. Cytopathic changes of…

Advisors/Committee Members: 대학원 의학과, 200624502, 박, 창은.

Subjects/Keywords: Naegleria fowleri; primary amoebic meningoencephalitis; microglial cells; cytopathic effect; cytotoxicity; pro-inflammatory cytokine

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

박, �. (2008). Cell death and Pro-inflammatory Cytokine Release of Rat Microglial Cells by Naegleria fowleri. (Thesis). Ajou University. Retrieved from http://repository.ajou.ac.kr/handle/201003/1403 ; http://dcoll.ajou.ac.kr:9080/dcollection/jsp/common/DcLoOrgPer.jsp?sItemId=000000009136

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

박, 창은. “Cell death and Pro-inflammatory Cytokine Release of Rat Microglial Cells by Naegleria fowleri.” 2008. Thesis, Ajou University. Accessed April 16, 2021. http://repository.ajou.ac.kr/handle/201003/1403 ; http://dcoll.ajou.ac.kr:9080/dcollection/jsp/common/DcLoOrgPer.jsp?sItemId=000000009136.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

박, 창은. “Cell death and Pro-inflammatory Cytokine Release of Rat Microglial Cells by Naegleria fowleri.” 2008. Web. 16 Apr 2021.

Vancouver:

박 �. Cell death and Pro-inflammatory Cytokine Release of Rat Microglial Cells by Naegleria fowleri. [Internet] [Thesis]. Ajou University; 2008. [cited 2021 Apr 16]. Available from: http://repository.ajou.ac.kr/handle/201003/1403 ; http://dcoll.ajou.ac.kr:9080/dcollection/jsp/common/DcLoOrgPer.jsp?sItemId=000000009136.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

박 �. Cell death and Pro-inflammatory Cytokine Release of Rat Microglial Cells by Naegleria fowleri. [Thesis]. Ajou University; 2008. Available from: http://repository.ajou.ac.kr/handle/201003/1403 ; http://dcoll.ajou.ac.kr:9080/dcollection/jsp/common/DcLoOrgPer.jsp?sItemId=000000009136

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

9. Suzuki, Celina Kassumi Kunieda. AVALIAÇÃO DE POLIMORFISMOS GENÉTICOS DE SUSCEPTIBILIDADE À OBESIDADE ASSOCIADOS AO PERFIL MASTIGATÓRIO DE CRIANÇAS OBESAS.

Degree: 2012, Pontifícia Universidade Católica de Goiás; Genética; PUC Goiás; BR; Ciências Humanas

URL: http://localhost:8080/tede/handle/tede/2365

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Made available in DSpace on 2016-08-10T10:38:42Z (GMT). No. of bitstreams: 1 CELINA KASSUMI KUNIEDA SUZUKI.pdf: 2013145 bytes, checksum: c807ebe49c872194bb7af7cd6bb5d17f (MD5) Previous issue date: 2012-06-29

The… (more)

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Made available in DSpace on 2016-08-10T10:38:42Z (GMT). No. of bitstreams: 1 CELINA KASSUMI KUNIEDA SUZUKI.pdf: 2013145 bytes, checksum: c807ebe49c872194bb7af7cd6bb5d17f (MD5) Previous issue date: 2012-06-29

The increased prevalence of childhood obesity in recent years suggests the existence of a genetic predisposition or susceptibility to conditioning factors for obesity which act on the environmental factors related to lifestyle involving diet and physical activity. Food education programs and physical activities have been developed in order to reverse this situation. The chewing is considered one of the early stages of the digestive process and therefore primordial important because performing correctly promotes natural hunger satiety as well as a healthy digestion. The aim of this study is to investigate the polymorphism of the TNF-α and DRD2 genes and the influence of masticatory profile in childhood obesity to enable the preparation of proposals for more effective interventions to control weight gain. For this purpose the study has been divided into two articles. ARTICLE 1: This study evaluated the profile of the obese child chewing through survey and evaluation of clinical history miofunctional from the protocols of MgBr and AMIOFE-A (attached) in 11 normal children representing the control population (Group I) and 16 obese children (Group B) participating in the CAIS Group of Obesity in Goiânia Municipal and School of Clinical Speech Therapy PUC Goiás aged between 6 and 13 years for both sexes. According to the findings obtained in the research, it is concluded that obese group (OB) presents an incision chewing food made with the central and lateral incisors with the majority labial sealing with or without excessive movement, unilateral pattern, with higher presence of altered behaviors in chewing and shorter chewing time. ARTICLE 2: This study evaluated the genetic polymorphism of TNF-α gene (G308A) and DRD2 (Taq1α - C32806T) in 27 patients with childhood obesity (16) and controls (11) using the method ARMS-PCR and PCR-RFLP, respectively, in peripheral blood samples. The results suggest that the genetic polymorphism of Taq1α (C32806T) in the DRD2 gene was associated with the increase of obesity in the childhood. Still genetic variants of the SNP G308A TNF-α gene is not possible to confirm it s influence on children s weight gain. Understanding how genetic variations affect the tendency to become or remain obese is an important step to comprehend the mechanisms that lead to obesity.

O aumento da prevalência da obesidade infantil nestes últimos anos sugere a existência de predisposição ou suscetibilidade genética como fatores condicionantes para a obesidade, sobre a qual atuam fatores ambientais relacionados ao estilo de vida, que envolvem hábitos alimentares e atividade física. Programas de educação alimentar e de atividades físicas foram desenvolvidos visando reverter este quadro. A mastigação é considerada uma das fases iniciais do processo digestório e de primordial…

Advisors/Committee Members: Silva, Cláudio Carlos da, Souza, Cejana Baiocchi, Silva, Daniela de Melo e.

Subjects/Keywords: Mastigação; Obesidade Infantil; Fonoaudiologia; Genética; Susceptibilidade; SNPs; Citocina pró-inflamatória; Dopamina; Mastication; Childhood Obesity; Speech Therapy; Genetics; Susceptibility, SNPs, pro-inflammatory cytokine; Dopamine; CNPQ::CIENCIAS BIOLOGICAS::GENETICA

Record Details Similar Records Cite « Share

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Suzuki, C. K. K. (2012). AVALIAÇÃO DE POLIMORFISMOS GENÉTICOS DE SUSCEPTIBILIDADE À OBESIDADE ASSOCIADOS AO PERFIL MASTIGATÓRIO DE CRIANÇAS OBESAS. (Masters Thesis). Pontifícia Universidade Católica de Goiás; Genética; PUC Goiás; BR; Ciências Humanas. Retrieved from http://localhost:8080/tede/handle/tede/2365

Chicago Manual of Style (16^th Edition):

Suzuki, Celina Kassumi Kunieda. “AVALIAÇÃO DE POLIMORFISMOS GENÉTICOS DE SUSCEPTIBILIDADE À OBESIDADE ASSOCIADOS AO PERFIL MASTIGATÓRIO DE CRIANÇAS OBESAS.” 2012. Masters Thesis, Pontifícia Universidade Católica de Goiás; Genética; PUC Goiás; BR; Ciências Humanas. Accessed April 16, 2021. http://localhost:8080/tede/handle/tede/2365.

MLA Handbook (7^th Edition):

Suzuki, Celina Kassumi Kunieda. “AVALIAÇÃO DE POLIMORFISMOS GENÉTICOS DE SUSCEPTIBILIDADE À OBESIDADE ASSOCIADOS AO PERFIL MASTIGATÓRIO DE CRIANÇAS OBESAS.” 2012. Web. 16 Apr 2021.

Vancouver:

Suzuki CKK. AVALIAÇÃO DE POLIMORFISMOS GENÉTICOS DE SUSCEPTIBILIDADE À OBESIDADE ASSOCIADOS AO PERFIL MASTIGATÓRIO DE CRIANÇAS OBESAS. [Internet] [Masters thesis]. Pontifícia Universidade Católica de Goiás; Genética; PUC Goiás; BR; Ciências Humanas; 2012. [cited 2021 Apr 16]. Available from: http://localhost:8080/tede/handle/tede/2365.

Council of Science Editors:

Suzuki CKK. AVALIAÇÃO DE POLIMORFISMOS GENÉTICOS DE SUSCEPTIBILIDADE À OBESIDADE ASSOCIADOS AO PERFIL MASTIGATÓRIO DE CRIANÇAS OBESAS. [Masters Thesis]. Pontifícia Universidade Católica de Goiás; Genética; PUC Goiás; BR; Ciências Humanas; 2012. Available from: http://localhost:8080/tede/handle/tede/2365

Northeastern University

10. Hosur, Vishnu. Genetic regulation of endoplasmic reticulum chaperones and pro-inflammatory cytokines by neuronal alpha4beta2 nicotinic receptors.

Degree: PhD, School of Pharmacy, 2009, Northeastern University

URL: http://hdl.handle.net/2047/d20000672

► Alpha4beta2 nicotinic acetylcholine receptors play important roles in the reward pathways for nicotine. We investigated whether receptor upregulation of alpha4beta2 receptors involves expression changes for… (more)

▼ Alpha4beta2 nicotinic acetylcholine receptors play important roles in the reward pathways for nicotine. We investigated whether receptor upregulation of alpha4beta2 receptors involves expression changes for non-receptor genes. In a microarray analysis, 10 microM nicotine altered expression of 41 genes at 0.25, 1, 8 and 24h in halpha4beta2 SHEP1 cells. Half of the genes were related to endoplasmic reticulum (ER) chaperones and the remaining genes belonged to inflammation and immune response pathways. The first objective was screening genes that alter surface alpha4beta2 expression using correlation analysis and RNA interference. Antagonists potentiate nicotine-induced alpha4beta2 upregulation, and correlation analysis showed that antagonists alone or in combination with nicotine suppressed an ER chaperone CRELD2 message while increasing surface expressing alpha4beta2 receptors. siRNA knockdown of CRELD2 increased basal alpha4beta2 receptor expression, and antagonists alone decreased CRELD2 mRNA in wild type SHEP1 cells lacking alpha4beta2 receptors. These data suggest that ER proteins such as CRELD2 decreases surface alpha4beta2 expression, and may explain antagonist actions in nicotine-induced receptor upregulation. The second objective was investigating the signaling pathways downstream of alpha4beta2 receptors leading to suppression of immune responses. Nicotine suppresses inflammatory cytokines and chemokines in halpha4beta2 SHEP1 cells but not in wild type SHEP1 cells. Quantitative RT-PCR (qPCR) corroborated nicotinic suppression of pro-inflammatory cytokines (PICs) IL-1beta and IL-6. 10 microM nicotine suppressed basal IL-1beta and IL-6 protein expression by blocking NFkB translocation. Nicotine dose-dependently attenuated lipopolysaccharide (LPS)-induced NFkB translocation, IkB-alpha phosphorylation and PIC production. A cell-permeable calcium chelator BAPTA-AM, adenylate cyclase stimulant forskolin and a specific PKA inhibitor PKI 14-22 AMIDE failed to block the effects of nicotine on LPS-induced NFkB translocation and IkB-alpha phosphorylation. The specific JAK2 inhibitor AG-490 and STAT3 inhibitor NSC74859 significantly blocked the anti-inflammatory effects of nicotine. These findings reveal a calcium-and cAMP-PKA independent signaling cascade and suggest a role for JAK2-STAT3 transduction in alpha4beta2-mediated anti-inflammatory actions against endotoxin-induced inflammation. Nicotine exposure decreased PIC production while upregulating alpha4beta2 receptors. This negative association between nicotine-induced increases in alpha4beta2 receptors and immune suppression may explain the neuroprotective effects observed in chronic smokers against neurodegenerative diseases such as Alzheimer's and Parkinson's disease.

Subjects/Keywords: health sciences; alpha4beta2 upregulation; JAK2-STAT3; neurodegeneration; nicotinic acetylcholine receptor; nuclear factor kappa B; pro-inflammatory cytokine; Cytokines; Endoplasmic reticulum; Molecular chaperones; Nicotinic receptors; Pharmacology

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Hosur, V. (2009). Genetic regulation of endoplasmic reticulum chaperones and pro-inflammatory cytokines by neuronal alpha4beta2 nicotinic receptors. (Doctoral Dissertation). Northeastern University. Retrieved from http://hdl.handle.net/2047/d20000672

Chicago Manual of Style (16^th Edition):

Hosur, Vishnu. “Genetic regulation of endoplasmic reticulum chaperones and pro-inflammatory cytokines by neuronal alpha4beta2 nicotinic receptors.” 2009. Doctoral Dissertation, Northeastern University. Accessed April 16, 2021. http://hdl.handle.net/2047/d20000672.

MLA Handbook (7^th Edition):

Hosur, Vishnu. “Genetic regulation of endoplasmic reticulum chaperones and pro-inflammatory cytokines by neuronal alpha4beta2 nicotinic receptors.” 2009. Web. 16 Apr 2021.

Vancouver:

Hosur V. Genetic regulation of endoplasmic reticulum chaperones and pro-inflammatory cytokines by neuronal alpha4beta2 nicotinic receptors. [Internet] [Doctoral dissertation]. Northeastern University; 2009. [cited 2021 Apr 16]. Available from: http://hdl.handle.net/2047/d20000672.

Council of Science Editors:

Hosur V. Genetic regulation of endoplasmic reticulum chaperones and pro-inflammatory cytokines by neuronal alpha4beta2 nicotinic receptors. [Doctoral Dissertation]. Northeastern University; 2009. Available from: http://hdl.handle.net/2047/d20000672

11. Natek, Maja. Kooperation zwischen Toll-like Rezeptoren und Sphingosin 1-phosphat in Keratinozyten.

Degree: 2017, Freie Universität Berlin

URL: https://refubium.fu-berlin.de/handle/fub188/12426

► Die Keratinozyten sind essenzielle Bestandteile der Epidermis, die auch eine Überwachungsrolle in der epidermalen Immunfunktion spielen. Auch als untypische Immunzellen sind sie in der Lage,… (more)

▼ Die Keratinozyten sind essenzielle Bestandteile der Epidermis, die auch eine Überwachungsrolle in der epidermalen Immunfunktion spielen. Auch als untypische Immunzellen sind sie in der Lage, schnell und effizient auf die Pathogene zu antworten und bilden damit einen Teil der ersten Reihe der angeborenen epidermalen Immunabwehr. Nach Enttarnung durch die Pathogene oder einen anderen Schaden der epidermalen Barriere reagieren die Keratinozyten mit einer verstärkten Entzündungsreaktion, die darüber hinaus eine Signalkaskade aktiviert, um die Barriere schnellstmöglich zu reparieren und den Wirt vor größeren Schäden zu schützen. Pathogen-assoziierte molekulare Strukturen (PAMPs) sind spezifische Teile der Mikroben, die nach der Bindung an die Pathogenstruktur-Erkennungsrezeptoren (PRRs) eine kritische Warnung für die Keratinozyten darstellen. Diese Rezeptoren sind gezielt in den verschiedenen Zelltypen exprimiert, was einer effizienten Früherkennung und Abwehr von gefährlichen Infektionen dient. Die Toll-like Rezeptoren (TLRs) sind die meist erforschte Gruppe der PRRs und sind in den Keratinozyten an der Zelloberfläche (TLR1,2,4,5,6) und in den intrazellulären Kompartimenten (TLR 3,9) zu finden. Die PAMP-Bindung an die TLRs verursacht eine Änderung der Rezeptorkonfirmation und aktiviert dadurch die zellulären Signalkaskaden, wodurch eine reichliche Freisetzung der pro-inflammatorischen Moleküle wie CXCL8 und IL-6 gefördert wird. Die TLR Funktionalität in den Keratinozyten wurde bereits bestätigt, jedoch ist ihre Bedeutung in der epidermalen Homöostase noch nicht vollständig bekannt. Demzufolge wurde in der vorliegenden Arbeit mit Hilfe der spezifischen TLR Agonisten die Beteiligung der TLR an der Entzündungsregulierung, dem Zellwachstum und der Zelldifferenzierung in normalen und modifizierten Keratinozyten untersucht. Die Aktivierung von TLR 2,3 und 9 erhöht die Freisetzung der CXL8, IL-1α und TNF. Des Weiteren konnte die TLR9-abhängige pro-inflammatorische Wirkung bestätigt werden. Zudem wurde der Effekt der längeren (24mer) Oligonukleotiden CpG und deren Kontrolle GpC, die eine Phosphothio-Bindung enthalten (PS-ODN), in den normalen humanen Keratinozyten und epithelischen Zelllinien HaCaT, SCC-12 und SCC-25 untersucht. Alle Zelltypen reagierten mit einer erhöhten Expression und Produktion der pro-inflammatorischen Zytokine (CXCL8, IL-1α und TNF). Interessanterweise hat die Blockade des TLR9 durch Chloroquine und siRNA Knockdown des TLR9 die CXCL8 Freisetzung reduziert. Dies deutet darauf hin, dass die pro-inflammatorische Wirkung der CpG-und GpC-ODN TLR9-abhängig ist. Es wurde auch gezeigt, dass CpG- und GpC-ODN keinen Einfluss auf die Zellviabilität der normalen Keratinozyten haben, wohingegen sie aber die Viabilität und das Wachstum der modifizierten und malignen Keratinozyten beeinflussten. Somit wurde dieser anti-proliferative Effekt am stärksten bei den HaCaT und SCC-25 Zellen beobachtet, welche nicht mittels Chloroquine Blockade unterbrochen wurden, was auf eine TLR9-unabhängige Wirkung hindeutet. Dahingegen… Advisors/Committee Members: n (gender), Prof. Dr. Günther Weindl (firstReferee), Prof. Dr. med. Margitta Worm (furtherReferee).

Subjects/Keywords: Toll-like receptor; sphingosine 1-phosphate; cytokine; keratinocytes; pro-inflammatory response; 500 Naturwissenschaften und Mathematik::570 Biowissenschaften; Biologie::570 Biowissenschaften; Biologie

…proteins and pro-inflammatory cytokine release to promote the defense against pathogens. Figure… …101 4.3.1 S1P pro-inflammatory signaling is S1PR1-3 independent… …103 4.4.1 S1P enhances TLR pro-inflammatory signaling in keratinocytes… …pro-inflammatory molecules like cytokines (IL-1 , TNF, IL-6) and chemokines (… …cells by a rapid secretion of pro-inflammatory cytokines and chemokines. Any impairment of the…

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APA (6^th Edition):

Natek, M. (2017). Kooperation zwischen Toll-like Rezeptoren und Sphingosin 1-phosphat in Keratinozyten. (Thesis). Freie Universität Berlin. Retrieved from https://refubium.fu-berlin.de/handle/fub188/12426

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Natek, Maja. “Kooperation zwischen Toll-like Rezeptoren und Sphingosin 1-phosphat in Keratinozyten.” 2017. Thesis, Freie Universität Berlin. Accessed April 16, 2021. https://refubium.fu-berlin.de/handle/fub188/12426.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Natek, Maja. “Kooperation zwischen Toll-like Rezeptoren und Sphingosin 1-phosphat in Keratinozyten.” 2017. Web. 16 Apr 2021.

Vancouver:

Natek M. Kooperation zwischen Toll-like Rezeptoren und Sphingosin 1-phosphat in Keratinozyten. [Internet] [Thesis]. Freie Universität Berlin; 2017. [cited 2021 Apr 16]. Available from: https://refubium.fu-berlin.de/handle/fub188/12426.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Natek M. Kooperation zwischen Toll-like Rezeptoren und Sphingosin 1-phosphat in Keratinozyten. [Thesis]. Freie Universität Berlin; 2017. Available from: https://refubium.fu-berlin.de/handle/fub188/12426

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Open Access Theses and Dissertations