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You searched for subject:(pri miRNA). Showing records 1 – 3 of 3 total matches.

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University of Technology, Sydney

1. Connerty, Patrick Peter. Investigating the regulation of miRNA biogenesis and Argonaute2 by RNA binding proteins.

Degree: 2016, University of Technology, Sydney

microRNAs (miRNAs) are small non-coding RNAs which post-transcriptionally regulate gene expression. As miRNAs control many important biological processes it is important that their own production is highly controlled too. A range of auxiliary proteins involved in regulating miRNA biogenesis have been documented extensively, highlighting the complexity of the miRNA pathway. This study identifies novel roles of RNA binding proteins which are both canonical and auxiliary to the miRNA biogenesis pathway. Here, we demonstrate that inhibition of p72 and KHSRP decreases Ago2 protein stability through disturbing miRNA biogenesis and therefore miRNA abundance. Furthermore, we have demonstrated that Ago2 is subject to multiple types of regulation as transient knockdown of Dicer stabilises Ago2 protein despite a decrease in miRNA abundance via an unknown mechanism. Additionally, we have established that miRNA biogenesis is subject to a possible negative feedback mechanisms in which impairment of Dicer function both promotes and inhibits pri-miRNA production in a pri-miRNA and cell specific manner. Finally, this study provides evidence to suggest that both mature miRNA levels and miRNA target abundance can stabilise miRNA biogenesis and promote pri-miRNA production in the absence of key and auxiliary proteins involved in miRNA biogenesis.

Subjects/Keywords: microRNAs (miRNAs); miRNA biogenesis.; RNA binding proteins.; p72 and KHSRP.; Ago2 protein stability.; Dicer.; pri-miRNA.

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6th Edition):

Connerty, P. P. (2016). Investigating the regulation of miRNA biogenesis and Argonaute2 by RNA binding proteins. (Thesis). University of Technology, Sydney. Retrieved from http://hdl.handle.net/10453/90062

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16th Edition):

Connerty, Patrick Peter. “Investigating the regulation of miRNA biogenesis and Argonaute2 by RNA binding proteins.” 2016. Thesis, University of Technology, Sydney. Accessed January 22, 2020. http://hdl.handle.net/10453/90062.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7th Edition):

Connerty, Patrick Peter. “Investigating the regulation of miRNA biogenesis and Argonaute2 by RNA binding proteins.” 2016. Web. 22 Jan 2020.

Vancouver:

Connerty PP. Investigating the regulation of miRNA biogenesis and Argonaute2 by RNA binding proteins. [Internet] [Thesis]. University of Technology, Sydney; 2016. [cited 2020 Jan 22]. Available from: http://hdl.handle.net/10453/90062.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Connerty PP. Investigating the regulation of miRNA biogenesis and Argonaute2 by RNA binding proteins. [Thesis]. University of Technology, Sydney; 2016. Available from: http://hdl.handle.net/10453/90062

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation


University of California – San Diego

2. Mondol, Vanessa. MicroRNAs in the Making: Post-transcriptional Regulation of MicroRNA Biogenesis in Caenorhabditis elegans.

Degree: Biology, 2015, University of California – San Diego

MicroRNAs (miRNAs) are small non-coding RNAs, ~22 nucleotides (nt) long, with major roles in gene regulation. MiRNAs bind imperfectly to complementary sequences in the 3’ untranslated region of target messenger RNAs (mRNAs) causing translational repression and destabilization. A single miRNA has the potential to regulate hundreds of different mRNA targets, highlighting the importance of miRNAs in almost all cellular pathways. Originally discovered as part of the Caenorhabditis elegans (C. elegans) developmental timing pathway, miRNAs were soon found in a multitude of other organisms, including humans. MiRBase, an online database for miRNAs, now lists >35,000 miRNAs, in >200 different species, including viruses, though many of their roles remain to be characterized. Because misregulation is often associated with disease, especially cancer, exploring miRNA biogenesis is critical for understanding the intricacies of disease development. Furthermore, the conserved temporal expression of various miRNAs highlights the importance of these regulators in pluripotency and development. Understanding how miRNAs are produced and regulated has been a major topic of study over the past 15 years. While the basic mechanisms of miRNA biogenesis and function have been uncovered, how these processes are regulated remains an outstanding problem.There are multiple instances of transcriptional and post-transcriptional regulation during miRNA biogenesis. In Chapter I, I introduce much of the latest understanding about the mechanisms of miRNA biogenesis and regulation. Details about the discovery of miRNAs, C. elegans as a model, as well as general information on biogenesis and targeting, can be found in Chapter II. I worked on several projects investigating post-transcriptional regulation of miRNA biogenesis in C. elegans. In Chapter III, I identify and characterize the primary lin-4 transcripts, and demonstrate how a conserved RNA binding protein, RBM-28, regulates mature lin-4 expression, but not primary or precursor. My investigation in Chapter IV led to notable insights on how splicing pri-let-7 leads to a secondary structure rearrangement that facilities recognition by the Microprocessor. My survey of polycistronic worm miRNAs discussed in Chapter V indicates that there are many more examples awaiting further study. Overall, this research describes novel examples of post-transcriptional regulation of miRNAs.

Subjects/Keywords: Biology; Molecular biology; Developmental biology; C. elegans; let-7; Microprocessor; microRNA; pri-miRNA; trans-splicing

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6th Edition):

Mondol, V. (2015). MicroRNAs in the Making: Post-transcriptional Regulation of MicroRNA Biogenesis in Caenorhabditis elegans. (Thesis). University of California – San Diego. Retrieved from http://www.escholarship.org/uc/item/3f93318b

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16th Edition):

Mondol, Vanessa. “MicroRNAs in the Making: Post-transcriptional Regulation of MicroRNA Biogenesis in Caenorhabditis elegans.” 2015. Thesis, University of California – San Diego. Accessed January 22, 2020. http://www.escholarship.org/uc/item/3f93318b.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7th Edition):

Mondol, Vanessa. “MicroRNAs in the Making: Post-transcriptional Regulation of MicroRNA Biogenesis in Caenorhabditis elegans.” 2015. Web. 22 Jan 2020.

Vancouver:

Mondol V. MicroRNAs in the Making: Post-transcriptional Regulation of MicroRNA Biogenesis in Caenorhabditis elegans. [Internet] [Thesis]. University of California – San Diego; 2015. [cited 2020 Jan 22]. Available from: http://www.escholarship.org/uc/item/3f93318b.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Mondol V. MicroRNAs in the Making: Post-transcriptional Regulation of MicroRNA Biogenesis in Caenorhabditis elegans. [Thesis]. University of California – San Diego; 2015. Available from: http://www.escholarship.org/uc/item/3f93318b

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation


Universitat Pompeu Fabra

3. Guidi, Mònica. Micro RNA-Mediated regulation of the full-length and truncated isoforms of human neurotrophic tyrosine kinase receptor type 3 (NTRK 3).

Degree: Departament de Ciències Experimentals i de la Salut, 2009, Universitat Pompeu Fabra

Las neurotrofinas y sus receptores constituyen una familia de factores cruciales para el desarrollo del sistema nervioso. La neurotrofina 3 ejerce su función principalmente a través de una unión de gran afinidad al receptor NTRK3, del cual se conocen dos isoformas principales, una larga de 150KDa con actividad de tipo tirosina kinasa y una truncada de 50KDa sin dicha actividad. Estas dos isoformas no comparten la misma región 3'UTR, lo que sugiere la existencia de una regulación postranscripcional diferente. En el presente trabajo se ha explorado como los microRNAs intervienen en la regulación de NTRK3, demostrando que las dos isoformas son reguladas por diferentes miRNAs. Se han analizado las consecuencias fisiológicas de la sobrexpresión de dichos microRNAs utilizando células de neuroblastoma. Finalmente, se ha estudiado la posible implicación del microRNA miR-124 en el control del splicing alternativo de NTRK3 a través de la regulación de represor de splicing PTBP1. Advisors/Committee Members: [email protected] (authoremail), true (authoremailshow), Espinosa Parrilla, Yolanda (director), Estivill, Xavier, 1955- (director).

Subjects/Keywords: RISC complex; retinoic acid; renilla luciferase; real-time quantitative PCR; PTBP1; pri-miRNA; pre-miRNA; post-transcriptional regulation; piRNA; PicTar; pGL4.13; PCR; P-body; p75NTR; overexpression; NTRK3; NTRK2; NTRK1; non-coding RNAs; NGF; neurotrophin; neurotrophic signaling; neuronal differentiation; neurodevelopment; neuroblastoma; nervous system; mRNA; miRNA target prediction; miRNA profiling; miRNA mimic; miRNA microarray; miRNA inhibitor; miRanda; microRNA; microarray; luciferase assay; Ingenuity Pathway Analysis (IPA); heLa cells; gene silencing; gene regulation; full-length isoform (FL-NTRK3); firefly luciferase; ERK; disease; dicer; cancer development; BDNF; argonaute; alternative splicing; 3'UTR; RNAhybrid; RT-PCR; SH-SY5Y cells; siRNA; standard error; synaptic plasticity; target validation; TargetScan; transfection; translational repression; TrkA; TrkB; TrkC; truncated isoform (TR-NTRK3); tyrosine kinase (TK); western blot; 575; 61

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6th Edition):

Guidi, M. (2009). Micro RNA-Mediated regulation of the full-length and truncated isoforms of human neurotrophic tyrosine kinase receptor type 3 (NTRK 3). (Thesis). Universitat Pompeu Fabra. Retrieved from http://hdl.handle.net/10803/7114

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16th Edition):

Guidi, Mònica. “Micro RNA-Mediated regulation of the full-length and truncated isoforms of human neurotrophic tyrosine kinase receptor type 3 (NTRK 3).” 2009. Thesis, Universitat Pompeu Fabra. Accessed January 22, 2020. http://hdl.handle.net/10803/7114.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7th Edition):

Guidi, Mònica. “Micro RNA-Mediated regulation of the full-length and truncated isoforms of human neurotrophic tyrosine kinase receptor type 3 (NTRK 3).” 2009. Web. 22 Jan 2020.

Vancouver:

Guidi M. Micro RNA-Mediated regulation of the full-length and truncated isoforms of human neurotrophic tyrosine kinase receptor type 3 (NTRK 3). [Internet] [Thesis]. Universitat Pompeu Fabra; 2009. [cited 2020 Jan 22]. Available from: http://hdl.handle.net/10803/7114.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Guidi M. Micro RNA-Mediated regulation of the full-length and truncated isoforms of human neurotrophic tyrosine kinase receptor type 3 (NTRK 3). [Thesis]. Universitat Pompeu Fabra; 2009. Available from: http://hdl.handle.net/10803/7114

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

.