subject:(polyamines)

University of Georgia

1. Liu, Qian. Biogeochemical cycling of polyamines in a coastal marine environment.

Degree: PhD, Marine Sciences, 2016, University of Georgia

URL: http://purl.galileo.usg.edu/uga_etd/liu_qian_201605_phd

► Polyamines are a group of short-chain aliphatic compounds containing multiple primary or secondary amine groups. They are ubiquitous in aquatic environments with concentrations in the… (more)

▼ Polyamines are a group of short-chain aliphatic compounds containing multiple primary or secondary amine groups. They are ubiquitous in aquatic environments with concentrations in the sub-nanomolar range, while concentrations in cells are at millimolar levels. They are commonly regarded as important osmolytes, synthesized or assimilated by osmotrophs, like bacterioplankton. They play a key role in cellular growth and synthesis of nucleic acids and proteins. However, few studies have examined the role of polyamines in oceanic N cycling. In this study, I hypothesized that polyamines contributed a significant amount of nitrogen to bacterial N production because of greater N: C ratio in polyamines than other labile organic nitrogen compounds. I determined turnover rates of three polyamines (putrescine, spermidine and spermine) in water samples using 3H-labeled compounds and measured their concentrations by HPLC in the South Atlantic Bight (SAB) and in salt marsh estuaries. The data showed that polyamines were rapidly assimilated by bacterioplankton, especially in samples from salt marshes and the inner-shelf of the SAB. However, low ambient concentrations of polyamines limited their contribution to bacterioplankton C and N demands compared to dissolved free amino acids that were used as controls. My study of polyamine metabolism in phytoplankton suggested that bacterial uptake of polyamines was limited by sources of polyamines. I found low concentrations (nmol L-1) of dissolved polyamine pools in media of phytoplankton cultures, similar to concentrations measured in the field. My data suggested that the composition and concentration of dissolved polyamines was a result of low diffusion from intracellular pools, selective release and uptake by healthy phytoplankton cells, as well as modifications by bacterioplankton uptake. Thaumarchaeota have been shown to oxidize polyamine nitrogen. This dissertation investigated the spatial and seasonal variability of abundances and activity of Thaumarchaeota in the SAB by quantitative PCR of Thaumarchaeota 16S rRNA and Archaea ammonia monooxygenase subunit A (amoA) genes and simulated in situ incubations with 15NH4Cl. I found a peak in Thaumarchaeota abundance (>1000-fold increase) in mid-summer at inshore and nearshore stations, but rapid ammonia oxidation was constrained to the inner-shelf at the mouth of the estuaries, probably driven by the substrate availability. Advisors/Committee Members: James T. Hollibaugh.

Subjects/Keywords: Polyamines

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APA (6^th Edition):

Liu, Q. (2016). Biogeochemical cycling of polyamines in a coastal marine environment. (Doctoral Dissertation). University of Georgia. Retrieved from http://purl.galileo.usg.edu/uga_etd/liu_qian_201605_phd

Chicago Manual of Style (16^th Edition):

Liu, Qian. “Biogeochemical cycling of polyamines in a coastal marine environment.” 2016. Doctoral Dissertation, University of Georgia. Accessed August 24, 2019. http://purl.galileo.usg.edu/uga_etd/liu_qian_201605_phd.

MLA Handbook (7^th Edition):

Liu, Qian. “Biogeochemical cycling of polyamines in a coastal marine environment.” 2016. Web. 24 Aug 2019.

Vancouver:

Liu Q. Biogeochemical cycling of polyamines in a coastal marine environment. [Internet] [Doctoral dissertation]. University of Georgia; 2016. [cited 2019 Aug 24]. Available from: http://purl.galileo.usg.edu/uga_etd/liu_qian_201605_phd.

Council of Science Editors:

Liu Q. Biogeochemical cycling of polyamines in a coastal marine environment. [Doctoral Dissertation]. University of Georgia; 2016. Available from: http://purl.galileo.usg.edu/uga_etd/liu_qian_201605_phd

Oregon State University

2. Rathi, Mahesh Shrikant. Application of polyallylamine as a dry strength agent.

Degree: MS, Forest Products, 1999, Oregon State University

URL: http://hdl.handle.net/1957/18414

► Dry strength is an inherent structural property of a paper sheet. It is due primarily to the development of fiber-to-fiber bonds. Polyamines, especially polyallylamine·HC1 (PAA),… (more)

Subjects/Keywords: Polyamines

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APA (6^th Edition):

Rathi, M. S. (1999). Application of polyallylamine as a dry strength agent. (Masters Thesis). Oregon State University. Retrieved from http://hdl.handle.net/1957/18414

Chicago Manual of Style (16^th Edition):

Rathi, Mahesh Shrikant. “Application of polyallylamine as a dry strength agent.” 1999. Masters Thesis, Oregon State University. Accessed August 24, 2019. http://hdl.handle.net/1957/18414.

MLA Handbook (7^th Edition):

Rathi, Mahesh Shrikant. “Application of polyallylamine as a dry strength agent.” 1999. Web. 24 Aug 2019.

Vancouver:

Rathi MS. Application of polyallylamine as a dry strength agent. [Internet] [Masters thesis]. Oregon State University; 1999. [cited 2019 Aug 24]. Available from: http://hdl.handle.net/1957/18414.

Council of Science Editors:

Rathi MS. Application of polyallylamine as a dry strength agent. [Masters Thesis]. Oregon State University; 1999. Available from: http://hdl.handle.net/1957/18414

University of Guelph

3. Krause, Michael. Sterically Demanding Ethylenediamines and Polyamines for the Stabilization of Main Group Elements in Low Coordination Numbers .

Degree: 2011, University of Guelph

URL: https://atrium.lib.uoguelph.ca/xmlui/handle/10214/3148

► This thesis explores the reaction of primary amines with 1,2-dibromoethane and 1,3-dibromopropane as a one step synthesis of N,N"-disubstituted ethylenediamines, RNH- CH2CH2-NH-R, N,N"-disubstituted propanediamines, R-NH-CH2CH2CH2-NH-R… (more)

Subjects/Keywords: ethylenediamines; polyamines

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APA (6^th Edition):

Krause, M. (2011). Sterically Demanding Ethylenediamines and Polyamines for the Stabilization of Main Group Elements in Low Coordination Numbers . (Thesis). University of Guelph. Retrieved from https://atrium.lib.uoguelph.ca/xmlui/handle/10214/3148

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Krause, Michael. “Sterically Demanding Ethylenediamines and Polyamines for the Stabilization of Main Group Elements in Low Coordination Numbers .” 2011. Thesis, University of Guelph. Accessed August 24, 2019. https://atrium.lib.uoguelph.ca/xmlui/handle/10214/3148.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Krause, Michael. “Sterically Demanding Ethylenediamines and Polyamines for the Stabilization of Main Group Elements in Low Coordination Numbers .” 2011. Web. 24 Aug 2019.

Vancouver:

Krause M. Sterically Demanding Ethylenediamines and Polyamines for the Stabilization of Main Group Elements in Low Coordination Numbers . [Internet] [Thesis]. University of Guelph; 2011. [cited 2019 Aug 24]. Available from: https://atrium.lib.uoguelph.ca/xmlui/handle/10214/3148.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Krause M. Sterically Demanding Ethylenediamines and Polyamines for the Stabilization of Main Group Elements in Low Coordination Numbers . [Thesis]. University of Guelph; 2011. Available from: https://atrium.lib.uoguelph.ca/xmlui/handle/10214/3148

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Aberdeen

4. Acheampong, Paul. Polyamines : effect of diet on blood concentrations and evaluation of their role as biomarkers of stroke.

Degree: 2010, University of Aberdeen

URL: http://digitool.abdn.ac.uk:80/webclient/DeliveryManager?pid=158358 ; http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.531842

► The main aims were to: optimise the method for extracting polyamine from food and blood products; study the effect of diet (after oral loading with… (more)

Subjects/Keywords: 612.3; Polyamines : Stroke

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APA (6^th Edition):

Acheampong, P. (2010). Polyamines : effect of diet on blood concentrations and evaluation of their role as biomarkers of stroke. (Doctoral Dissertation). University of Aberdeen. Retrieved from http://digitool.abdn.ac.uk:80/webclient/DeliveryManager?pid=158358 ; http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.531842

Chicago Manual of Style (16^th Edition):

Acheampong, Paul. “Polyamines : effect of diet on blood concentrations and evaluation of their role as biomarkers of stroke.” 2010. Doctoral Dissertation, University of Aberdeen. Accessed August 24, 2019. http://digitool.abdn.ac.uk:80/webclient/DeliveryManager?pid=158358 ; http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.531842.

MLA Handbook (7^th Edition):

Acheampong, Paul. “Polyamines : effect of diet on blood concentrations and evaluation of their role as biomarkers of stroke.” 2010. Web. 24 Aug 2019.

Vancouver:

Acheampong P. Polyamines : effect of diet on blood concentrations and evaluation of their role as biomarkers of stroke. [Internet] [Doctoral dissertation]. University of Aberdeen; 2010. [cited 2019 Aug 24]. Available from: http://digitool.abdn.ac.uk:80/webclient/DeliveryManager?pid=158358 ; http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.531842.

Council of Science Editors:

Acheampong P. Polyamines : effect of diet on blood concentrations and evaluation of their role as biomarkers of stroke. [Doctoral Dissertation]. University of Aberdeen; 2010. Available from: http://digitool.abdn.ac.uk:80/webclient/DeliveryManager?pid=158358 ; http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.531842

Montana State University

5. Woller, Eric Kevin. Mannose-functionalized PAMAM dendrimers : their synthesis, characterization and use in refining the model of protein-carbohydrate interactions.

Degree: College of Letters & Science, 2003, Montana State University

URL: https://scholarworks.montana.edu/xmlui/handle/1/8368

Subjects/Keywords: Polyamines.; Glycosides.

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APA (6^th Edition):

Woller, E. K. (2003). Mannose-functionalized PAMAM dendrimers : their synthesis, characterization and use in refining the model of protein-carbohydrate interactions. (Thesis). Montana State University. Retrieved from https://scholarworks.montana.edu/xmlui/handle/1/8368

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Woller, Eric Kevin. “Mannose-functionalized PAMAM dendrimers : their synthesis, characterization and use in refining the model of protein-carbohydrate interactions.” 2003. Thesis, Montana State University. Accessed August 24, 2019. https://scholarworks.montana.edu/xmlui/handle/1/8368.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Woller, Eric Kevin. “Mannose-functionalized PAMAM dendrimers : their synthesis, characterization and use in refining the model of protein-carbohydrate interactions.” 2003. Web. 24 Aug 2019.

Vancouver:

Woller EK. Mannose-functionalized PAMAM dendrimers : their synthesis, characterization and use in refining the model of protein-carbohydrate interactions. [Internet] [Thesis]. Montana State University; 2003. [cited 2019 Aug 24]. Available from: https://scholarworks.montana.edu/xmlui/handle/1/8368.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Woller EK. Mannose-functionalized PAMAM dendrimers : their synthesis, characterization and use in refining the model of protein-carbohydrate interactions. [Thesis]. Montana State University; 2003. Available from: https://scholarworks.montana.edu/xmlui/handle/1/8368

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Aberdeen

6. Acheampong, Paul. Polyamines.

Degree: School of Medicine and Dentistry., 2010, University of Aberdeen

URL: http://digitool.abdn.ac.uk:80/webclient/DeliveryManager?application=DIGITOOL-3&owner=resourcediscovery&custom_att_2=simple_viewer&pid=158358 ; http://digitool2.abdn.ac.uk:1801/webclient/DeliveryManager?pid=158358&custom_att_2=simple_viewer

Subjects/Keywords: Polyamines.; Stroke.

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Acheampong, P. (2010). Polyamines. (Doctoral Dissertation). University of Aberdeen. Retrieved from http://digitool.abdn.ac.uk:80/webclient/DeliveryManager?application=DIGITOOL-3&owner=resourcediscovery&custom_att_2=simple_viewer&pid=158358 ; http://digitool2.abdn.ac.uk:1801/webclient/DeliveryManager?pid=158358&custom_att_2=simple_viewer

Chicago Manual of Style (16^th Edition):

Acheampong, Paul. “Polyamines.” 2010. Doctoral Dissertation, University of Aberdeen. Accessed August 24, 2019. http://digitool.abdn.ac.uk:80/webclient/DeliveryManager?application=DIGITOOL-3&owner=resourcediscovery&custom_att_2=simple_viewer&pid=158358 ; http://digitool2.abdn.ac.uk:1801/webclient/DeliveryManager?pid=158358&custom_att_2=simple_viewer.

MLA Handbook (7^th Edition):

Acheampong, Paul. “Polyamines.” 2010. Web. 24 Aug 2019.

Vancouver:

Acheampong P. Polyamines. [Internet] [Doctoral dissertation]. University of Aberdeen; 2010. [cited 2019 Aug 24]. Available from: http://digitool.abdn.ac.uk:80/webclient/DeliveryManager?application=DIGITOOL-3&owner=resourcediscovery&custom_att_2=simple_viewer&pid=158358 ; http://digitool2.abdn.ac.uk:1801/webclient/DeliveryManager?pid=158358&custom_att_2=simple_viewer.

Council of Science Editors:

Acheampong P. Polyamines. [Doctoral Dissertation]. University of Aberdeen; 2010. Available from: http://digitool.abdn.ac.uk:80/webclient/DeliveryManager?application=DIGITOOL-3&owner=resourcediscovery&custom_att_2=simple_viewer&pid=158358 ; http://digitool2.abdn.ac.uk:1801/webclient/DeliveryManager?pid=158358&custom_att_2=simple_viewer

University of Arizona

7. Vargas, Ashley Joy. Assessing the Role of Dietary Polyamines on the Continuum of Colorectal Carcinoma .

Degree: 2013, University of Arizona

URL: http://hdl.handle.net/10150/293416

► Putrescine, spermidine and spermine are the polyamines biosynthesized by human cells via ornithine decarboxylase (ODC) and are also sourced from the diet. Polyamines are required… (more)

▼ Putrescine, spermidine and spermine are the polyamines biosynthesized by human cells via ornithine decarboxylase (ODC) and are also sourced from the diet. Polyamines are required for malignant and normal cell growth and development. Pharmacological suppression of polyamine biosynthesis, by difluoromethylornithine, and inflammation, via sulindac, has demonstrated ~70% efficacy in preventing premalignant colorectal adenomas (CRA) in a clinical trial; however, high polyamine intakes mitigated this preventative action. Further, dietary polyamines increase the dysplasia of CRA in initiated animal models of colorectal cancer (CRC) and are hypothesized to function as tumor promoters. Human research on dietary polyamines was limited until the development of a dietary database in 2007 but, continues to be limited by the lack of a biomarker of exposure. Chapter 1 of this dissertation tests the hypothesis that dietary polyamines increase risk of CRA in polyp-formers (n = 1164) and found evidence to support this hypothesis. However, only women, younger participants and certain genotypes experienced more risk of CRA with high polyamine exposure. Chapter II tests the hypothesis that dietary polyamines increase the risk for CRC in an average risk cohort of post-menopausal women (n = 87,620) and did not find evidence to support this hypothesis in the whole population. Rather, dietary polyamines were non-significantly protective against CRC and significantly protective when paired with aspirin use and against CRC-specific death. There was some evidence to support an increase in risk of CRC in younger participants with high polyamine exposure. Overall, the first two chapters suggest that dietary polyamines protect the colorectum in normal risk individuals but promote carcinogenesis in high risk individuals. Chapter III tests the hypothesis that dietary polyamine intake correlates with urinary polyamine output in a group of overweight/obese, older men (n = 36) and Chapter IV tests the hypothesis that intake of highly ripe sweet cherries will increase urinary polyamine output in a subgroup of 10 men from Chapter III. The findings from these chapters suggest there may be a positive correlation, but that a better measure of dietary polyamine intake is needed to determine if urinary polyamines are biomarkers of exposure to polyamines. Advisors/Committee Members: Thompson, Patricia A (advisor), Thompson, Patricia A. (committeemember), Thompson, Cynthia A. (committeemember), Gerner, Eugene W. (committeemember), Romagnolo, Donato F. (committeemember), Schroeder, Joyce A. (committeemember).

Subjects/Keywords: Colorectal cancer; Dietary polyamines; Polyamines; Putrescine; Spermidine; Nutritional Sciences; Colorectal adenoma

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APA (6^th Edition):

Vargas, A. J. (2013). Assessing the Role of Dietary Polyamines on the Continuum of Colorectal Carcinoma . (Doctoral Dissertation). University of Arizona. Retrieved from http://hdl.handle.net/10150/293416

Chicago Manual of Style (16^th Edition):

Vargas, Ashley Joy. “Assessing the Role of Dietary Polyamines on the Continuum of Colorectal Carcinoma .” 2013. Doctoral Dissertation, University of Arizona. Accessed August 24, 2019. http://hdl.handle.net/10150/293416.

MLA Handbook (7^th Edition):

Vargas, Ashley Joy. “Assessing the Role of Dietary Polyamines on the Continuum of Colorectal Carcinoma .” 2013. Web. 24 Aug 2019.

Vancouver:

Vargas AJ. Assessing the Role of Dietary Polyamines on the Continuum of Colorectal Carcinoma . [Internet] [Doctoral dissertation]. University of Arizona; 2013. [cited 2019 Aug 24]. Available from: http://hdl.handle.net/10150/293416.

Council of Science Editors:

Vargas AJ. Assessing the Role of Dietary Polyamines on the Continuum of Colorectal Carcinoma . [Doctoral Dissertation]. University of Arizona; 2013. Available from: http://hdl.handle.net/10150/293416

8. Ramani, David. Citrulline et métabolisme des polyamines : quelle place pour la N-carbamoylputrescine? : Citrulline and polyamine metabolism : what role for N-carbamoylputrescine ?.

Degree: Docteur es, Nutrition, 2012, Université Paris Descartes – Paris V

URL: http://www.theses.fr/2012PA05P632

►

La citrulline est un acide aminé dont les propriétés anaboliques n’ont été mises en évidence que très récemment. Ces propriétés pourraient relever en particulier de… (more)

▼

La citrulline est un acide aminé dont les propriétés anaboliques n’ont été mises en évidence que très récemment. Ces propriétés pourraient relever en particulier de son rôle de précurseur direct de l’arginine. L’arginine est un acide aminé central du métabolisme des mammifères, notamment comme précurseur de nombreuses molécules exerçant des fonctions physiologiques essentielles, en particulier les polyamines, que ce soit les polyamines « classiques » putrescine, spermidine et spermine, dérivés de décarboxylation de l’ornithine impliquées entre autres dans la prolifération cellulaire, ou l’agmatine, dérivé de la décarboxylation de l’arginine. Toutefois, les interactions métaboliques entre citrulline et polyamines restent encore peu connues. Le but de ce travail a été d’explorer le lien métabolique entre citrulline et polyamines, selon deux démarches distinctes.La première a été d’évaluer l’influence d’une complémentation prolongée (3 mois) en citrulline sur le métabolisme tissulaire des polyamines « classiques » chez le rat âgé. La citrulline n’affecte que de manière modérée le métabolisme des polyamines, principalement en corrigeant les altérations liées au vieillissement.La seconde, par analogie avec la putrescine et l’agmatine, a exploré l’implication du dérivé décarboxylé de la citrulline, la N-carbamoyl-putrescine (NCP) dans le métabolisme des mammifères après avoir mis au point une technique de dosage de celle-ci dans les milieux biologiques. Nous n’avons pas pu mettre en évidence la présence de NCP dans des tissus de rats ayant reçu de la citrulline par voie orale ou par voie parentérale suggèrant soit une absence de synthèse soit un métabolisme extrêmement rapide de la NCP. Par ailleurs, la NCP administrée par voie orale à des doses de 5 et 50 mg/kg/j ne semble pas induire de toxicité ni d’effets biologiques majeurs mais la démonstration de son absorption digestive reste à faire. Cependant, la NCP exerce quelques effets modérés sur le métabolisme des acides aminés et des polyamines, l’évaluation de doses plus élevées ou l’administration par voie parentérale permettrait de confirmer ces observations.

The anabolic properties of the amino acid citrulline have only been recently demonstrated. These properties may result at least in part from its role as an arginine precursor. Arginine plays a central role in mammalian amino acid metabolism, notably as the precusor of many molecules involved in essential physiological functions, such as polyamines either “classical” ones, namely putrescine, spermidine, and spermine, derived from ornithine decarboxylation and notably involved in cell proliferation, or more recently-discovered molecules such as agmatine, the decarboxylation derivative of arginine. Metabolic interactions between citrulline and polyamines are still mostly unknown.The aim of this work was to explore the metabolic link between citrulline and polyamines using two different strategies.First, the influence of a long term citrulline supplementation on tissue “classical” polyamine metabolism in aged rats…

Advisors/Committee Members: Bandt, Jean-Pascal de (thesis director).

Subjects/Keywords: N-Carbamoylputrescine; Polyamines; Citrulline; Metabolisme; 572.465

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APA (6^th Edition):

Ramani, D. (2012). Citrulline et métabolisme des polyamines : quelle place pour la N-carbamoylputrescine? : Citrulline and polyamine metabolism : what role for N-carbamoylputrescine ?. (Doctoral Dissertation). Université Paris Descartes – Paris V. Retrieved from http://www.theses.fr/2012PA05P632

Chicago Manual of Style (16^th Edition):

Ramani, David. “Citrulline et métabolisme des polyamines : quelle place pour la N-carbamoylputrescine? : Citrulline and polyamine metabolism : what role for N-carbamoylputrescine ?.” 2012. Doctoral Dissertation, Université Paris Descartes – Paris V. Accessed August 24, 2019. http://www.theses.fr/2012PA05P632.

MLA Handbook (7^th Edition):

Ramani, David. “Citrulline et métabolisme des polyamines : quelle place pour la N-carbamoylputrescine? : Citrulline and polyamine metabolism : what role for N-carbamoylputrescine ?.” 2012. Web. 24 Aug 2019.

Vancouver:

Ramani D. Citrulline et métabolisme des polyamines : quelle place pour la N-carbamoylputrescine? : Citrulline and polyamine metabolism : what role for N-carbamoylputrescine ?. [Internet] [Doctoral dissertation]. Université Paris Descartes – Paris V; 2012. [cited 2019 Aug 24]. Available from: http://www.theses.fr/2012PA05P632.

Council of Science Editors:

Ramani D. Citrulline et métabolisme des polyamines : quelle place pour la N-carbamoylputrescine? : Citrulline and polyamine metabolism : what role for N-carbamoylputrescine ?. [Doctoral Dissertation]. Université Paris Descartes – Paris V; 2012. Available from: http://www.theses.fr/2012PA05P632

University of Pretoria

9. Van Brummelen, Anna Catharina. Functional genomics analysis of the effects of co-inhibition of the malarial S-adenosylmethionine decarboxylase/ornithine decarboxylase.

Degree: Biochemistry, 2009, University of Pretoria

URL: http://hdl.handle.net/2263/25135

► Polyamines are ubiquitous components of all living cells and their depletion usually causes growth arrest or cytostasis, a strategy employed for treatment of West-African trypanosomiasis.… (more)

▼ Polyamines are ubiquitous components of all living cells and their depletion usually causes growth arrest or cytostasis, a strategy employed for treatment of West-African trypanosomiasis. In the malaria parasite, Plasmodium falciparum, polyamine biosynthesis is regulated by the uniquely bifunctional protein, Sadenosylmethionine decarboxylase/ornithine decarboxylase (PfAdoMetDC/ODC). The unique nature of this protein could provide a selective mechanism for antimalarial treatment. To validate polyamine depletion and specifically PfAdoMetDC/ODC, as drug target for antimalarial therapeutic intervention, polyamine biosynthesis was completely restrained via the inhibition of both catalytic sites of PfAdoMetDC/ODC with DFMO and MDL73811. The physiological effects during the resulting cytostasis were studied with a comprehensive functional genomics approach. The study was preceded by various assays to determine the treatment dosage that would result in complete cytostasis, without non-specific chemical cytotoxicity. The results obtained revealed that the cytostatic mechanism with growth arrest of the treated parasites and normal progression of the untreated controls require special consideration for basic comparisons of response in terms of the assay methodology used and data analysis. This is particularly important when studying a multistage organism such as P. falciparum, which constantly develops and change during the intraerythrocytic developmental cycle, such that growth arrest compared to normal progression would result in significant differences merely due to stage. This critical principle was kept in mind throughout the investigation and was applied to the relative quantification of RNA, proteins and metabolites via a relative time zero approach as opposed to the standard parallel time point comparison. Three independent functional genomics investigations, namely transcriptomics, proteomics and metabolomics were conducted, in which highly synchronised 3D7 parasite cultures were treated during the schizont stage and parasites were sampled during a time course at three time points (just before and during cytostasis). Transcriptome analysis revealed the occurrence of a generalised transcriptional arrest just prior to the growth arrest. To our knowledge this is the first time that transcriptional arrest as the preceding mechanism of cytostasis due to polyamine depletion, was demonstrated. However, despite the transcriptional arrest, the abundance of 538 transcripts was differentially affected and included three perturbation-specific compensatory transcriptional responses: the increased abundance of the transcripts for lysine decarboxylase and ornithine aminotransferase (OAT) and the decreased abundance of that for S-adenosylmethionine synthetase (AdoMet synthetase). Pearson correlations indicated more subtle effects of the perturbation on the proteome and even more so on the metabolome where homeostasis was generally maintained, except downstream to the enzymatic blockade at PfAdoMetDC/ODC. The perturbation-specific… Advisors/Committee Members: Dr L Birkholtz (advisor), Prof A I Louw (advisor), Prof M Llinas (advisor).

Subjects/Keywords: P. falciparum; Malaria parasite; Polyamines; UCTD

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APA (6^th Edition):

Van Brummelen, A. (2009). Functional genomics analysis of the effects of co-inhibition of the malarial S-adenosylmethionine decarboxylase/ornithine decarboxylase. (Doctoral Dissertation). University of Pretoria. Retrieved from http://hdl.handle.net/2263/25135

Chicago Manual of Style (16^th Edition):

Van Brummelen, Anna. “Functional genomics analysis of the effects of co-inhibition of the malarial S-adenosylmethionine decarboxylase/ornithine decarboxylase.” 2009. Doctoral Dissertation, University of Pretoria. Accessed August 24, 2019. http://hdl.handle.net/2263/25135.

MLA Handbook (7^th Edition):

Van Brummelen, Anna. “Functional genomics analysis of the effects of co-inhibition of the malarial S-adenosylmethionine decarboxylase/ornithine decarboxylase.” 2009. Web. 24 Aug 2019.

Vancouver:

Van Brummelen A. Functional genomics analysis of the effects of co-inhibition of the malarial S-adenosylmethionine decarboxylase/ornithine decarboxylase. [Internet] [Doctoral dissertation]. University of Pretoria; 2009. [cited 2019 Aug 24]. Available from: http://hdl.handle.net/2263/25135.

Council of Science Editors:

Van Brummelen A. Functional genomics analysis of the effects of co-inhibition of the malarial S-adenosylmethionine decarboxylase/ornithine decarboxylase. [Doctoral Dissertation]. University of Pretoria; 2009. Available from: http://hdl.handle.net/2263/25135

NSYSU

10. Wang, Kuo-Cheng. Polyamines affect early floral initiation in Doritis pulcherrima and Phalaenopsis.

Degree: Master, Biological Sciences, 2002, NSYSU

URL: http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0711102-163013

► Polyamine titer in the leaves of the self-seedlings of Doritis pulcherrima Lindleyï¼cv. S84-3352ï¼under short-day ( SD, 9 hours of light period ) and long-day (… (more)

Subjects/Keywords: Doritis; polyamines; Phalaenopsis

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APA (6^th Edition):

Wang, K. (2002). Polyamines affect early floral initiation in Doritis pulcherrima and Phalaenopsis. (Thesis). NSYSU. Retrieved from http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0711102-163013

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Wang, Kuo-Cheng. “Polyamines affect early floral initiation in Doritis pulcherrima and Phalaenopsis.” 2002. Thesis, NSYSU. Accessed August 24, 2019. http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0711102-163013.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Wang, Kuo-Cheng. “Polyamines affect early floral initiation in Doritis pulcherrima and Phalaenopsis.” 2002. Web. 24 Aug 2019.

Vancouver:

Wang K. Polyamines affect early floral initiation in Doritis pulcherrima and Phalaenopsis. [Internet] [Thesis]. NSYSU; 2002. [cited 2019 Aug 24]. Available from: http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0711102-163013.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Wang K. Polyamines affect early floral initiation in Doritis pulcherrima and Phalaenopsis. [Thesis]. NSYSU; 2002. Available from: http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0711102-163013

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Pretoria

11. [No author]. Functional genomics analysis of the effects of co-inhibition of the malarial S-adenosylmethionine decarboxylase/ornithine decarboxylase .

Degree: 2009, University of Pretoria

URL: http://upetd.up.ac.za/thesis/available/etd-05302009-124548/

► Polyamines are ubiquitous components of all living cells and their depletion usually causes growth arrest or cytostasis, a strategy employed for treatment of West-African trypanosomiasis.… (more)

▼ Polyamines are ubiquitous components of all living cells and their depletion usually causes growth arrest or cytostasis, a strategy employed for treatment of West-African trypanosomiasis. In the malaria parasite, Plasmodium falciparum, polyamine biosynthesis is regulated by the uniquely bifunctional protein, Sadenosylmethionine decarboxylase/ornithine decarboxylase (PfAdoMetDC/ODC). The unique nature of this protein could provide a selective mechanism for antimalarial treatment. To validate polyamine depletion and specifically PfAdoMetDC/ODC, as drug target for antimalarial therapeutic intervention, polyamine biosynthesis was completely restrained via the inhibition of both catalytic sites of PfAdoMetDC/ODC with DFMO and MDL73811. The physiological effects during the resulting cytostasis were studied with a comprehensive functional genomics approach. The study was preceded by various assays to determine the treatment dosage that would result in complete cytostasis, without non-specific chemical cytotoxicity. The results obtained revealed that the cytostatic mechanism with growth arrest of the treated parasites and normal progression of the untreated controls require special consideration for basic comparisons of response in terms of the assay methodology used and data analysis. This is particularly important when studying a multistage organism such as P. falciparum, which constantly develops and change during the intraerythrocytic developmental cycle, such that growth arrest compared to normal progression would result in significant differences merely due to stage. This critical principle was kept in mind throughout the investigation and was applied to the relative quantification of RNA, proteins and metabolites via a relative time zero approach as opposed to the standard parallel time point comparison. Three independent functional genomics investigations, namely transcriptomics, proteomics and metabolomics were conducted, in which highly synchronised 3D7 parasite cultures were treated during the schizont stage and parasites were sampled during a time course at three time points (just before and during cytostasis). Transcriptome analysis revealed the occurrence of a generalised transcriptional arrest just prior to the growth arrest. To our knowledge this is the first time that transcriptional arrest as the preceding mechanism of cytostasis due to polyamine depletion, was demonstrated. However, despite the transcriptional arrest, the abundance of 538 transcripts was differentially affected and included three perturbation-specific compensatory transcriptional responses: the increased abundance of the transcripts for lysine decarboxylase and ornithine aminotransferase (OAT) and the decreased abundance of that for S-adenosylmethionine synthetase (AdoMet synthetase). Pearson correlations indicated more subtle effects of the perturbation on the proteome and even more so on the metabolome where homeostasis was generally maintained, except downstream to the enzymatic blockade at PfAdoMetDC/ODC. The perturbation-specific… Advisors/Committee Members: Dr L Birkholtz (advisor), Prof A I Louw (advisor), Prof M Llinas (advisor).

Subjects/Keywords: P. falciparum; Malaria parasite; Polyamines; UCTD

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APA (6^th Edition):

author], [. (2009). Functional genomics analysis of the effects of co-inhibition of the malarial S-adenosylmethionine decarboxylase/ornithine decarboxylase . (Doctoral Dissertation). University of Pretoria. Retrieved from http://upetd.up.ac.za/thesis/available/etd-05302009-124548/

Chicago Manual of Style (16^th Edition):

author], [No. “Functional genomics analysis of the effects of co-inhibition of the malarial S-adenosylmethionine decarboxylase/ornithine decarboxylase .” 2009. Doctoral Dissertation, University of Pretoria. Accessed August 24, 2019. http://upetd.up.ac.za/thesis/available/etd-05302009-124548/.

MLA Handbook (7^th Edition):

author], [No. “Functional genomics analysis of the effects of co-inhibition of the malarial S-adenosylmethionine decarboxylase/ornithine decarboxylase .” 2009. Web. 24 Aug 2019.

Vancouver:

author] [. Functional genomics analysis of the effects of co-inhibition of the malarial S-adenosylmethionine decarboxylase/ornithine decarboxylase . [Internet] [Doctoral dissertation]. University of Pretoria; 2009. [cited 2019 Aug 24]. Available from: http://upetd.up.ac.za/thesis/available/etd-05302009-124548/.

Council of Science Editors:

author] [. Functional genomics analysis of the effects of co-inhibition of the malarial S-adenosylmethionine decarboxylase/ornithine decarboxylase . [Doctoral Dissertation]. University of Pretoria; 2009. Available from: http://upetd.up.ac.za/thesis/available/etd-05302009-124548/

Georgia Tech

12. Hartong, Bradley H. Epi-halohydrin modified polyamines as mordant agents for alkaline rosin sizing.

Degree: PhD, Institute of Paper Science and Technology, 2004, Georgia Tech

URL: http://hdl.handle.net/1853/7032

► Many grades of paper have shifted from acid to alkaline production conditions. This change excludes the usage of traditional alum-rosin sizing chemistry. In some cases… (more)

▼ Many grades of paper have shifted from acid to alkaline production conditions. This change excludes the usage of traditional alum-rosin sizing chemistry. In some cases however, rosin sizing could prove beneficial if it could be used under neutral to alkaline paper making conditions. One effort toward this goal has been the use of epi-halohydrin modified polyamine/polyaminoamide in place of alum. The optimum sizing conditions, as well as the mechanism that modified polyamines/polyaminoamides use to size rosin, is investigated. Polyethyleneimine modified with epi-chlorohydrin was used in these studies. Rosin dosage was found to be the main variable that controlled the degree of sizing achieved. As the only hydrophobic material added, this was expected. In addition to rosin dosage, the polymer and pH had significant impacts on this sizing chemistry. Laboratory data shows that the synthesis procedure for and structure of polyethyleneimine-epi-cholohydrin (PEI-epi) impacts the degree of sizing achieved. Use of different modification techniques and degrees of modification illustrated several key factory regarding the polymer as a rosin sizing mordant. Prevention or removal of by-products from epi-chlorohydrin imporves the sizing efficiency of PEI-epi with rosin. A critical molecular weight of the PEI-epi was found to be important to sizing. Higher charge density on PEI-epi provides better the sizing efficiency. In addition to the polymer, other important factors where examined. The pH of the stock impacted the degree of sizing and permanency of the sizing achieved. Varying stock pH showed changes in sizing that are consistent with effects seen for rosin retention and of PAE as a wet strength agent. Low and high pH levels also caused reversion of the sizing effect. A pilot trial of the PEI-epi-rosin sizing chemistry was conducted at the Herty Foundation. The pilot trial shows that PEI-epi-rosin can reach the required level of sizing for linerboard. Trial results showed that calcium carbonate filled sheets could be sized by this chemistry. No significant impact on strength is seen from the sizing chemistry at the required dosage levels. As would be expected, addition of filler diminishes sheet strength. No sizing reversion was seen from any samples of this trial. Size reversion at low stock pH indicated that acid catalytic cleavage of an ester bond could have been occurring. Addition of acid or base to permanently sized sheets showed significant levels of reversion, similar to that seen for low stock pH sheets. This provides further supporting eviden ce for the formation and importance of an ester bond between rosin and PEI-epi. FTIR spectra were collected for model compounds to further examine the presence and importance of ester bonds. An ester bond was found to form at room temperature with aging or immediately with drying. Further, the ester bonds were found to decrease when the sample was subjected to acidic or basic… Advisors/Committee Members: Yulin Deng, Ph.D. Assoc. Professor - Georgia Tech (School, Arthur Ragauskas (Committee), Lucian Lucia, Sujit Banerjee, and Jeffery Hsieh.

Subjects/Keywords: Alkalinity; Sizing; Polyamines

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APA (6^th Edition):

Hartong, B. H. (2004). Epi-halohydrin modified polyamines as mordant agents for alkaline rosin sizing. (Doctoral Dissertation). Georgia Tech. Retrieved from http://hdl.handle.net/1853/7032

Chicago Manual of Style (16^th Edition):

Hartong, Bradley H. “Epi-halohydrin modified polyamines as mordant agents for alkaline rosin sizing.” 2004. Doctoral Dissertation, Georgia Tech. Accessed August 24, 2019. http://hdl.handle.net/1853/7032.

MLA Handbook (7^th Edition):

Hartong, Bradley H. “Epi-halohydrin modified polyamines as mordant agents for alkaline rosin sizing.” 2004. Web. 24 Aug 2019.

Vancouver:

Hartong BH. Epi-halohydrin modified polyamines as mordant agents for alkaline rosin sizing. [Internet] [Doctoral dissertation]. Georgia Tech; 2004. [cited 2019 Aug 24]. Available from: http://hdl.handle.net/1853/7032.

Council of Science Editors:

Hartong BH. Epi-halohydrin modified polyamines as mordant agents for alkaline rosin sizing. [Doctoral Dissertation]. Georgia Tech; 2004. Available from: http://hdl.handle.net/1853/7032

13. Κουρνούτου, Γεωργία. Μεταβολές στα επίπεδα των πολυαμινών του πεπτικού αδένα του μυδιού Mytilus galloprovincialis υπό την επίδραση οξειδωτικού στρες.

Degree: 2013, University of Patras

URL: http://hdl.handle.net/10889/6237

►

Οι πολυαμίνες είναι μικρά κατιοντικά μόρια απαραίτητα για την ανάπτυξη και τη διαφοροποίηση των κυττάρων. Εμπλέκονται σε ένα μεγάλο αριθμό κυτταρικών διεργασιών και παίζουν σημαντικό… (more)

Subjects/Keywords: Πολυαμίνες; Οξειδωτικό στρες; 572.548; Polyamines; Oxidative stress

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APA (6^th Edition):

Κουρνούτου, �. (2013). Μεταβολές στα επίπεδα των πολυαμινών του πεπτικού αδένα του μυδιού Mytilus galloprovincialis υπό την επίδραση οξειδωτικού στρες. (Masters Thesis). University of Patras. Retrieved from http://hdl.handle.net/10889/6237

Chicago Manual of Style (16^th Edition):

Κουρνούτου, Γεωργία. “Μεταβολές στα επίπεδα των πολυαμινών του πεπτικού αδένα του μυδιού Mytilus galloprovincialis υπό την επίδραση οξειδωτικού στρες.” 2013. Masters Thesis, University of Patras. Accessed August 24, 2019. http://hdl.handle.net/10889/6237.

MLA Handbook (7^th Edition):

Κουρνούτου, Γεωργία. “Μεταβολές στα επίπεδα των πολυαμινών του πεπτικού αδένα του μυδιού Mytilus galloprovincialis υπό την επίδραση οξειδωτικού στρες.” 2013. Web. 24 Aug 2019.

Vancouver:

Κουρνούτου �. Μεταβολές στα επίπεδα των πολυαμινών του πεπτικού αδένα του μυδιού Mytilus galloprovincialis υπό την επίδραση οξειδωτικού στρες. [Internet] [Masters thesis]. University of Patras; 2013. [cited 2019 Aug 24]. Available from: http://hdl.handle.net/10889/6237.

Council of Science Editors:

Κουρνούτου �. Μεταβολές στα επίπεδα των πολυαμινών του πεπτικού αδένα του μυδιού Mytilus galloprovincialis υπό την επίδραση οξειδωτικού στρες. [Masters Thesis]. University of Patras; 2013. Available from: http://hdl.handle.net/10889/6237

University of Hong Kong

14. 丘國明; Yao, Kwok-ming. Purification and characterization of ornithine decarbozylase fromtetrahymena thermophila.

Degree: M. Phil., 1986, University of Hong Kong

URL: Yao, K. [丘國明]. (1986). Purification and characterization of ornithine decarbozylase fromtetrahymena thermophila. (Thesis). University of Hong Kong, Pokfulam, Hong Kong SAR. Retrieved from http://dx.doi.org/10.5353/th_b3120748 ; http://dx.doi.org/10.5353/th_b3120748 ; http://hdl.handle.net/10722/32901

published_or_final_version

Biochemistry

Master

Master of Philosophy

Subjects/Keywords: Polyamines.; Tetrahymena.; Decarboxylases.

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APA (6^th Edition):

丘國明; Yao, K. (1986). Purification and characterization of ornithine decarbozylase fromtetrahymena thermophila. (Masters Thesis). University of Hong Kong. Retrieved from Yao, K. [丘國明]. (1986). Purification and characterization of ornithine decarbozylase fromtetrahymena thermophila. (Thesis). University of Hong Kong, Pokfulam, Hong Kong SAR. Retrieved from http://dx.doi.org/10.5353/th_b3120748 ; http://dx.doi.org/10.5353/th_b3120748 ; http://hdl.handle.net/10722/32901

Chicago Manual of Style (16^th Edition):

丘國明; Yao, Kwok-ming. “Purification and characterization of ornithine decarbozylase fromtetrahymena thermophila.” 1986. Masters Thesis, University of Hong Kong. Accessed August 24, 2019. Yao, K. [丘國明]. (1986). Purification and characterization of ornithine decarbozylase fromtetrahymena thermophila. (Thesis). University of Hong Kong, Pokfulam, Hong Kong SAR. Retrieved from http://dx.doi.org/10.5353/th_b3120748 ; http://dx.doi.org/10.5353/th_b3120748 ; http://hdl.handle.net/10722/32901.

MLA Handbook (7^th Edition):

丘國明; Yao, Kwok-ming. “Purification and characterization of ornithine decarbozylase fromtetrahymena thermophila.” 1986. Web. 24 Aug 2019.

Vancouver:

丘國明; Yao K. Purification and characterization of ornithine decarbozylase fromtetrahymena thermophila. [Internet] [Masters thesis]. University of Hong Kong; 1986. [cited 2019 Aug 24]. Available from: Yao, K. [丘國明]. (1986). Purification and characterization of ornithine decarbozylase fromtetrahymena thermophila. (Thesis). University of Hong Kong, Pokfulam, Hong Kong SAR. Retrieved from http://dx.doi.org/10.5353/th_b3120748 ; http://dx.doi.org/10.5353/th_b3120748 ; http://hdl.handle.net/10722/32901.

Council of Science Editors:

丘國明; Yao K. Purification and characterization of ornithine decarbozylase fromtetrahymena thermophila. [Masters Thesis]. University of Hong Kong; 1986. Available from: Yao, K. [丘國明]. (1986). Purification and characterization of ornithine decarbozylase fromtetrahymena thermophila. (Thesis). University of Hong Kong, Pokfulam, Hong Kong SAR. Retrieved from http://dx.doi.org/10.5353/th_b3120748 ; http://dx.doi.org/10.5353/th_b3120748 ; http://hdl.handle.net/10722/32901

Montana State University

15. Hulla, Janis Elaine. Polyamine mutagenesis : evidence for a unique mechanism.

Degree: College of Letters & Science, 1981, Montana State University

URL: https://scholarworks.montana.edu/xmlui/handle/1/3652

Subjects/Keywords: Polyamines.; Chemical mutagenesis.

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APA (6^th Edition):

Hulla, J. E. (1981). Polyamine mutagenesis : evidence for a unique mechanism. (Thesis). Montana State University. Retrieved from https://scholarworks.montana.edu/xmlui/handle/1/3652

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Hulla, Janis Elaine. “Polyamine mutagenesis : evidence for a unique mechanism.” 1981. Thesis, Montana State University. Accessed August 24, 2019. https://scholarworks.montana.edu/xmlui/handle/1/3652.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Hulla, Janis Elaine. “Polyamine mutagenesis : evidence for a unique mechanism.” 1981. Web. 24 Aug 2019.

Vancouver:

Hulla JE. Polyamine mutagenesis : evidence for a unique mechanism. [Internet] [Thesis]. Montana State University; 1981. [cited 2019 Aug 24]. Available from: https://scholarworks.montana.edu/xmlui/handle/1/3652.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Hulla JE. Polyamine mutagenesis : evidence for a unique mechanism. [Thesis]. Montana State University; 1981. Available from: https://scholarworks.montana.edu/xmlui/handle/1/3652

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

McGill University

16. Potvin, Diane. Synthesis of polyamines with potential affinity for the alpha-adrenoreceptor.

Degree: MS, Department of Chemistry., 1984, McGill University

URL: http://digitool.library.mcgill.ca/thesisfile65316.pdf

Subjects/Keywords: Polyamines.; Adrenaline – Receptors.

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APA (6^th Edition):

Potvin, D. (1984). Synthesis of polyamines with potential affinity for the alpha-adrenoreceptor. (Masters Thesis). McGill University. Retrieved from http://digitool.library.mcgill.ca/thesisfile65316.pdf

Chicago Manual of Style (16^th Edition):

Potvin, Diane. “Synthesis of polyamines with potential affinity for the alpha-adrenoreceptor.” 1984. Masters Thesis, McGill University. Accessed August 24, 2019. http://digitool.library.mcgill.ca/thesisfile65316.pdf.

MLA Handbook (7^th Edition):

Potvin, Diane. “Synthesis of polyamines with potential affinity for the alpha-adrenoreceptor.” 1984. Web. 24 Aug 2019.

Vancouver:

Potvin D. Synthesis of polyamines with potential affinity for the alpha-adrenoreceptor. [Internet] [Masters thesis]. McGill University; 1984. [cited 2019 Aug 24]. Available from: http://digitool.library.mcgill.ca/thesisfile65316.pdf.

Council of Science Editors:

Potvin D. Synthesis of polyamines with potential affinity for the alpha-adrenoreceptor. [Masters Thesis]. McGill University; 1984. Available from: http://digitool.library.mcgill.ca/thesisfile65316.pdf

Université de Sherbrooke

17. Benrezzak, Ouhida. Rôle de l'ornithine décarboxylase et des polyamines dans la croissance stimulée du pancréas exocrine par la caeruléine .

Degree: 1984, Université de Sherbrooke

URL: http://hdl.handle.net/11143/14907

► Cette recherche fut inspirée par les résultats de plusieurs travaux qui ont démontré que les hormones gastrointestinales, sécrétine, cholécystokinine et son analogue la caeruléine favorisaient… (more)

▼ Cette recherche fut inspirée par les résultats de plusieurs travaux qui ont démontré que les hormones gastrointestinales, sécrétine, cholécystokinine et son analogue la caeruléine favorisaient la croissance du pancréas exocrine et entreprise dans le but d'élucider le mode d'action de la caeruléine qui demeure encore inconnu. En raison de la forte association des polyamines (putrescine, spermidine et spermine) à la croissance cellulaire, nous avons vérifié si la caeruléine n'agirait pas via la synthèse accrue de ces polycations pour stimuler la croissance du pancréas exocrine. Pour ce faire, des rats (150-155 g) ont reçu dans l'eau de boisson, 2 jours avant et durant le traitement à la caeruléine (1 µg/kg de poids corporel) et de l'x DFMO (2% solution), un inhibiteur spécifique et irréversible de l'enzyme qui initie la biosynthèse des polyamines: l'ornithine décarboxylase (ODC). Après sacrifice, les pancréas furent prélevés, pesés et évalués quant à leurs contenus totaux en polyamines. Alors que l'hyperplasie fut déterminée par les mesures du contenu total de l'organe en ADN et par la synthèse de l'ADN, l'hypertrophie fut établie par l'évaluation de la masse cellulaire, des concentrations en enzymes, en protéines et en ARN. Les principaux points qui ont été étudiés et analysés furent: L'effet de la caeruléine seule et combinée au DFMO sur les contenus en polyamines du pancréas. L'effet de la caeruléine seule et combinée au DFMO sur les paramètres d'hyperplasie et d'hypertrophie (deux phases de la croissance). L'effet de l'administration des polyamines exogènes, notamment la putrescine sur la croissance du pancréas. Les résultats obtenus ont montré principalement que: La caeruléine augmente significativement les contenus en putrescine (319%) après 12 heures et de 217% après 24 heures de traitement. Le DFMO (2%) réduit et abolit ces augmentations après 12 et 24 heures de traitement. La spermidine et la spermine sont augmentées respectivement par 63% et 50% après 96 heures, période de déclin de la putrescine. Tandis que la spermidine fut inhibée par le DFM0, la spermine demeura inchangée. Des injections sous-cutanées aux 8 heures à la caeruléine ont provoqué des augmentations significatives de tous les paramètres d'hypertrophie et d'hyperplasie après 48 et 96 heures. Le DFMO seul n'affecte aucun de ces paramètres. L'inhibiteur de l'ODC est cependant capable de réduire la croissance stimulée du pancréas ayant réduit significativement: le poids pancréatique total et les indices d'hyperplasie après 48 et 96 heures des changements majeurs ont été parallèlement observés au niveau des concentrations de la chymotrypsine après 48 et 96 heures. Une simple et unique injection intrapéritonéale de putrescine à raison de 300 µmoles/kg/jour a complètement aboli les diminutions de l'ADN total causées par l' x-DFMO. Étant donné que les augmentations de putrescine semblent suivre celles de l'ADN, que l'inhibition de l'ODC, l'abolition et la réduction des niveaux en putrescine et spermidine ont affecté l'hyperplasie et que… Advisors/Committee Members: Morisset, Jean (advisor).

Subjects/Keywords: Ornithine décarboxylase; Polyamines

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APA (6^th Edition):

Benrezzak, O. (1984). Rôle de l'ornithine décarboxylase et des polyamines dans la croissance stimulée du pancréas exocrine par la caeruléine . (Masters Thesis). Université de Sherbrooke. Retrieved from http://hdl.handle.net/11143/14907

Chicago Manual of Style (16^th Edition):

Benrezzak, Ouhida. “Rôle de l'ornithine décarboxylase et des polyamines dans la croissance stimulée du pancréas exocrine par la caeruléine .” 1984. Masters Thesis, Université de Sherbrooke. Accessed August 24, 2019. http://hdl.handle.net/11143/14907.

MLA Handbook (7^th Edition):

Benrezzak, Ouhida. “Rôle de l'ornithine décarboxylase et des polyamines dans la croissance stimulée du pancréas exocrine par la caeruléine .” 1984. Web. 24 Aug 2019.

Vancouver:

Benrezzak O. Rôle de l'ornithine décarboxylase et des polyamines dans la croissance stimulée du pancréas exocrine par la caeruléine . [Internet] [Masters thesis]. Université de Sherbrooke; 1984. [cited 2019 Aug 24]. Available from: http://hdl.handle.net/11143/14907.

Council of Science Editors:

Benrezzak O. Rôle de l'ornithine décarboxylase et des polyamines dans la croissance stimulée du pancréas exocrine par la caeruléine . [Masters Thesis]. Université de Sherbrooke; 1984. Available from: http://hdl.handle.net/11143/14907

Brock University

18. Fixon-Owoo, Solomon N. K. Synthesis of 4-hydroxycinnamic amides of di-, tri-, and tetraamines : potential insect toxins .

Degree: Department of Chemistry, 2000, Brock University

URL: http://hdl.handle.net/10464/1345

► The monoconjugates of phenolic acids (i.e. coumaric acid) with polyamines such as spermidine and spermine are strikingly similar to some toxins from spiders and predatory… (more)

Subjects/Keywords: Polyamines; Amides; Insecticides.

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APA (6^th Edition):

Fixon-Owoo, S. N. K. (2000). Synthesis of 4-hydroxycinnamic amides of di-, tri-, and tetraamines : potential insect toxins . (Thesis). Brock University. Retrieved from http://hdl.handle.net/10464/1345

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Fixon-Owoo, Solomon N K. “Synthesis of 4-hydroxycinnamic amides of di-, tri-, and tetraamines : potential insect toxins .” 2000. Thesis, Brock University. Accessed August 24, 2019. http://hdl.handle.net/10464/1345.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Fixon-Owoo, Solomon N K. “Synthesis of 4-hydroxycinnamic amides of di-, tri-, and tetraamines : potential insect toxins .” 2000. Web. 24 Aug 2019.

Vancouver:

Fixon-Owoo SNK. Synthesis of 4-hydroxycinnamic amides of di-, tri-, and tetraamines : potential insect toxins . [Internet] [Thesis]. Brock University; 2000. [cited 2019 Aug 24]. Available from: http://hdl.handle.net/10464/1345.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Fixon-Owoo SNK. Synthesis of 4-hydroxycinnamic amides of di-, tri-, and tetraamines : potential insect toxins . [Thesis]. Brock University; 2000. Available from: http://hdl.handle.net/10464/1345

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

IUPUI

19. Liao, Chung-Ping. Polyamines and Alveolar Macrophage Apoptosis during Pneumocystis Pneumonia.

Degree: 2009, IUPUI

URL: http://hdl.handle.net/1805/1958

►

Indiana University-Purdue University Indianapolis (IUPUI)

Pneumocystis pneumonia (PCP) is the leading opportunistic disease in immunocompromised individuals, particularly in AIDS patients. The alveolar macrophage (AM) is… (more)

▼

Indiana University-Purdue University Indianapolis (IUPUI)

Pneumocystis pneumonia (PCP) is the leading opportunistic disease in immunocompromised individuals, particularly in AIDS patients. The alveolar macrophage (AM) is the major type of cell responsible for the clearance of Pneumocystis organisms; however, they undergo a high rate of apoptosis during PCP due to increased intracellular polyamine levels. This study examined the mechanism of this polyamine mediated apoptosis and investigated an alternative therapy for PCP by targeting this mechanism. The elevated polyamine levels were determined to be caused by increased polyamine synthesis and uptake. Increased polyamine uptake was found to be AM-specific, and recruited inflammatory cells including monocytes, B cells, and CD8+ T cells were found to be a potential source of polyamines. The expression of the antizyme inhibitor (AZI), which regulates both polyamine synthesis and uptake, was found to be greatly up-regulated in AMs during PCP. AZI overexpression was confirmed to be the cause of increased polyamine synthesis and uptake and apoptosis of AMs during PCP by gene knockdown assays. Pneumocystis organisms and zymosan were found to induce AZI overexpression in AMs, suggesting that the β-glucan of the Pneumocystis cell wall is responsible for this AZI up-regulation. In addition, levels of mRNA, protein, and activity of polyamine oxidase (PAO) were also found to be increased in AMs during PCP, and its substrates N1-acetylspermidine and N1-acetylspermine were found to induce its up-regulation. These results indicate that the H2O2 generated during PAO-mediated polyamine catabolism caused AMs to undergo apoptosis. Since increased polyamine uptake was demonstrated to be a pathogenic mechanism of PCP in this study, the potential therapeutic activity of five putative polyamine transport inhibitors against PCP was tested. Results showed that compound 44-Ant-44 significantly decreased pulmonary inflammation, organism burden, and macrophage apoptosis, and prolonged the survival of rats with PCP. In summary, this study demonstrated that Pneumocystis organisms induce AZI overexpression, leading to increased polyamine synthesis, uptake, and apoptosis rate in AMs and that targeting polyamine transport is a viable therapeutic approach against PCP.

Advisors/Committee Members: Lee, Chao-Hung, Lasbury, Mark E., Davis, Thomas E., Gregory, Richard L..

Subjects/Keywords: Polyamines, Alveolar Macrophage, Apoptosis, Pneumocystis Pneumonia; Polyamines; Macrophages; Apoptosis; Pneumocystis carinii pneumonia; HIV infections – Complications

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Liao, C. (2009). Polyamines and Alveolar Macrophage Apoptosis during Pneumocystis Pneumonia. (Thesis). IUPUI. Retrieved from http://hdl.handle.net/1805/1958

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Liao, Chung-Ping. “Polyamines and Alveolar Macrophage Apoptosis during Pneumocystis Pneumonia.” 2009. Thesis, IUPUI. Accessed August 24, 2019. http://hdl.handle.net/1805/1958.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Liao, Chung-Ping. “Polyamines and Alveolar Macrophage Apoptosis during Pneumocystis Pneumonia.” 2009. Web. 24 Aug 2019.

Vancouver:

Liao C. Polyamines and Alveolar Macrophage Apoptosis during Pneumocystis Pneumonia. [Internet] [Thesis]. IUPUI; 2009. [cited 2019 Aug 24]. Available from: http://hdl.handle.net/1805/1958.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Liao C. Polyamines and Alveolar Macrophage Apoptosis during Pneumocystis Pneumonia. [Thesis]. IUPUI; 2009. Available from: http://hdl.handle.net/1805/1958

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Université de Montréal

20. Lefèvre, Pavine L.C. Les voies de signalisation utérines à l'émergence de la diapause embryonnaire chez le vison américain .

Degree: 2011, Université de Montréal

URL: http://hdl.handle.net/1866/4611

► La diapause embryonnaire se manifeste par un arrêt réversible du développement embryonnaire durant la période de préimplantation et induit un retard de l’implantation. Chez le… (more)

▼ La diapause embryonnaire se manifeste par un arrêt réversible du développement embryonnaire durant la période de préimplantation et induit un retard de l’implantation. Chez le vison américain, une diapause embryonnaire obligatoire caractérise chaque gestation. Si les mécanismes de contrôle de la diapause embryonnaire obligatoire chez cette espèce sont bien connus, le rôle utérin impliqué dans la réactivation de l’embryon demeure, quant à lui, encore inconnu. Le sujet de ce doctorat a consisté dans un premier temps à explorer l’environnement utérin à la sortie de la diapause embryonnaire afin de caractériser, dans un deuxième temps, les principaux acteurs utérins qui provoquent la réactivation de l’embryon. Nous avons effectué une analyse du transcriptome utérin à l’émergence de la diapause embryonnaire ce qui a permis de construire une librairie de 123 séquences d’ADNc utérines différentiellement exprimées à la réactivation de l’embryon et homologues à des séquences de gènes connues chez d’autres espèces. Ces gènes sont impliqués dans la régulation du métabolisme (25 %), de l’expression génique (21 %), de la transduction de signal (15 %), du cycle cellulaire (15 %), du transport (10 %) et de la structure cellulaire (9 %), reflétant ainsi d’importantes modifications utérines à la réactivation embryonnaire. Nous avons validé l’expression différentielle de dix gènes ainsi identifiés : GDF3 (growth and differentiation 3), ALCAM (activated leukocyte cell adhesion molecule), ADIPOR1 (adiponectin receptor 1), HMGN1 (high mobility group N1), TXNL1 (thioredoxin like 1), TGM2 (tissue transglutaminase 2), SPARC (secreted protein acidic rich in cystein), et trois gènes codant pour AZIN1 (antizyme inhibitor 1), ODC1 (ornithine decarboxylase 1) et SAT1 (spermidine/spermine N1-acetyltransferase), des enzymes impliquées dans la biosynthèse des polyamines. Le patron de l’expression spatio-temporel de SPARC et d’HMGN1 illustrent spécifiquement un remodelage tissulaire et de la chromatine au niveau utérin à la sortie de la diapause embryonnaire. Ayant mesuré une augmentation des concentrations utérines en polyamines à la reprise du développement embryonnaire, nous avons émis l’hypothèse que les polyamines seraient impliquées dans les événements menant à la sortie de la diapause. L’inhibition de la biosynthèse des polyamines par un traitement à l’ α-difluoromethylornithine (DFMO) a provoqué une diminution significative de la proliferation cellulaire dans les embryons à la réactivation, un retard du moment de l’implantation, mais n’a pas affecté le succès de la reproduction. De manière similaire, nous avons induit un état de dormance dans les cellules de trophoblaste de vison en présence DFMO dans le milieu de culture, et constaté que cet état était réversible. En conclusion, cette étude a non seulement ouvert de nouveaux horizons quant à la compréhension du rôle utérin dans les événements menant à la sortie de la diapause embryonnaire, mais a démontré pour la première fois, l’existence de facteurs utérins… Advisors/Committee Members: Murphy, Bruce D (advisor).

Subjects/Keywords: diapause; blastocyste; trophoblaste; implantation; hybridation suppressive soustractive; polyamines; vison; diapause; blastocyst; trophoblast; uterus; implantation; suppressive subtractive hybridization; polyamines; mink; utérus

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Lefèvre, P. L. C. (2011). Les voies de signalisation utérines à l'émergence de la diapause embryonnaire chez le vison américain . (Thesis). Université de Montréal. Retrieved from http://hdl.handle.net/1866/4611

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Lefèvre, Pavine L C. “Les voies de signalisation utérines à l'émergence de la diapause embryonnaire chez le vison américain .” 2011. Thesis, Université de Montréal. Accessed August 24, 2019. http://hdl.handle.net/1866/4611.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Lefèvre, Pavine L C. “Les voies de signalisation utérines à l'émergence de la diapause embryonnaire chez le vison américain .” 2011. Web. 24 Aug 2019.

Vancouver:

Lefèvre PLC. Les voies de signalisation utérines à l'émergence de la diapause embryonnaire chez le vison américain . [Internet] [Thesis]. Université de Montréal; 2011. [cited 2019 Aug 24]. Available from: http://hdl.handle.net/1866/4611.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Lefèvre PLC. Les voies de signalisation utérines à l'émergence de la diapause embryonnaire chez le vison américain . [Thesis]. Université de Montréal; 2011. Available from: http://hdl.handle.net/1866/4611

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Florida

21. Geddes, Ryan D. Improving Fermentation of Pretreated Biomass by Reducing Inhibitor Concentrations and Increasing Microbial Biocatalyst Tolerance.

Degree: PhD, Microbiology and Cell Science, 2014, University of Florida

URL: http://ufdc.ufl.edu/UFE0047363

► Lignocellulosic biomass provides an abundant source of carbohydrates that can be utilized for the production of fuels and chemicals. However, plant biomass is resistant to… (more)

Subjects/Keywords: Biomass; Enzymes; Ethanol; Ethanol production; Fermentation; Plasmids; Polyamines; Pretreatment; Sugars; Toxicity; biomass – detoxify – ethanol – furfural – hydrolysate – inhibitors – lignocellulose – polyamines

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Geddes, R. D. (2014). Improving Fermentation of Pretreated Biomass by Reducing Inhibitor Concentrations and Increasing Microbial Biocatalyst Tolerance. (Doctoral Dissertation). University of Florida. Retrieved from http://ufdc.ufl.edu/UFE0047363

Chicago Manual of Style (16^th Edition):

Geddes, Ryan D. “Improving Fermentation of Pretreated Biomass by Reducing Inhibitor Concentrations and Increasing Microbial Biocatalyst Tolerance.” 2014. Doctoral Dissertation, University of Florida. Accessed August 24, 2019. http://ufdc.ufl.edu/UFE0047363.

MLA Handbook (7^th Edition):

Geddes, Ryan D. “Improving Fermentation of Pretreated Biomass by Reducing Inhibitor Concentrations and Increasing Microbial Biocatalyst Tolerance.” 2014. Web. 24 Aug 2019.

Vancouver:

Geddes RD. Improving Fermentation of Pretreated Biomass by Reducing Inhibitor Concentrations and Increasing Microbial Biocatalyst Tolerance. [Internet] [Doctoral dissertation]. University of Florida; 2014. [cited 2019 Aug 24]. Available from: http://ufdc.ufl.edu/UFE0047363.

Council of Science Editors:

Geddes RD. Improving Fermentation of Pretreated Biomass by Reducing Inhibitor Concentrations and Increasing Microbial Biocatalyst Tolerance. [Doctoral Dissertation]. University of Florida; 2014. Available from: http://ufdc.ufl.edu/UFE0047363

22. Hatmi, Saloua. Impact du déficit hydrique sur les réponses de défense et la sensibilité de la vigne à Botrytis cinerea : rôle de la dégradation des polyamines. : Impact of water deficiency on defense responses and susceptibility of grapevine to Botrytis cinerea : Role of polyamine oxidation.

Degree: Docteur es, Sciences - STS, 2013, Reims; Université de Tunis. Faculté des sciences de Tunis

URL: http://www.theses.fr/2013REIMS035

►

La vigne est sujette à de nombreuses contraintes biotiques et abiotiques face auxquelles elle devra optimiser ses stratégies de défense, en favorisant parfois des interconnections… (more)

▼

La vigne est sujette à de nombreuses contraintes biotiques et abiotiques face auxquelles elle devra optimiser ses stratégies de défense, en favorisant parfois des interconnections entre les réponses adaptatives au stress abiotique et la gestion de la réponse immune face à un pathogène. Dans cette étude nous avons évalué l'effet du stress hydrique (par privation d'eau) sur différentes réactions adaptatives au stress, mais aussi sur des réponses de défense et sur la sensibilité des feuilles et des baies de la vigne à Botrytis cinerea. Ces réactions ont été suivies en utilisant des boutures végétatives de deux cépages : cv. Meski (MSK), tolérant à la sécheresse et cv. Chardonnay (CHR), sensible. La relation entre les réponses au stress hydrique et la réponse immune a également été recherchée au moyen de feuilles de vitroplants et de baies isolées de Chardonnay exposées à des osmotica : le polyéthylène glycol (PEG) et une forte concentration en saccahrose (SUC). Les résultats montrent que le stress hydrique/osmotique conduit à des modifications physiologiques et biochimiques importantes dans les feuilles et les baies mûres de vigne. L'amélioration de la tolérance chez MSK est associée à une faible inhibition de l'activité photosynthétique, une altération du profil des acides aminés et un catabolisme actif des polyamines (PAs) comparé au CHR. Ces résultats suggèrent un rôle potentiel des déviations métaboliques observées dans les processus de tolérance de la vigne au stress osmotique. La tolérance du MSK au déficit hydrique est également corrélée à une forte induction des réponses de défense accumulation de resvératrol et e-viniférine, expression de certains gènes de défense dont STS, Gluc (PR-2), Chit-4c (PR-3) et PR-5 dans les feuilles, ainsi qu'à une faible sensibilité à B. cinerea.Ces résultats suggèrent un lien étroit entre la tolérance au déficit hydrique et la capacité de la vigne à exprimer davantage ses mécanismes de défense et à résister mieux à B. cinerea. Des expériences pharmacologiques ont montré qu'en situation de stress hydrique/osmotique, le catabolisme des PAs, via des diamine- et PA-oxydases, est impliqué dans la régulation de l'homéostasie des PAs et de l'expression des réactions de défense. L'application du stress osmotique avant l'infection des feuilles par B. cinerea potentialise l'accumulation des PAs en réduisant fortement leur dégradation. Ces effets sont corrélés à une réduction de l'amplitude des réponses de défense après infection, et à une sensibilité accrue à B. cinerea. Ces résultats rendent compte (1) de l'importance des stress abiotiques dans la régulation de la réponse immune chez la vigne et sa résistance à B. cinerea et (2) du fait que le niveau des réponses de défense osmo-induites et la résistance au pathogène sont tributaires, au moins en partie, de certains mécanismes adaptatifs au stress, comme c'est le cas ici des voies de dégradation des polyamines.

Grapevine is often exposed to both biotic and abiotic stresses, and it will optimize defense strategies by favoing sometimes the…

Advisors/Committee Members: Aziz, Aziz (thesis director).

Subjects/Keywords: Vigne; Stress hydrique; Botrytis cinerea; Polyamines; Réponse immune; Grapevine; Water stress; Botrytis cinerea; Polyamines; Immune responses; 634.8

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Hatmi, S. (2013). Impact du déficit hydrique sur les réponses de défense et la sensibilité de la vigne à Botrytis cinerea : rôle de la dégradation des polyamines. : Impact of water deficiency on defense responses and susceptibility of grapevine to Botrytis cinerea : Role of polyamine oxidation. (Doctoral Dissertation). Reims; Université de Tunis. Faculté des sciences de Tunis. Retrieved from http://www.theses.fr/2013REIMS035

Chicago Manual of Style (16^th Edition):

Hatmi, Saloua. “Impact du déficit hydrique sur les réponses de défense et la sensibilité de la vigne à Botrytis cinerea : rôle de la dégradation des polyamines. : Impact of water deficiency on defense responses and susceptibility of grapevine to Botrytis cinerea : Role of polyamine oxidation.” 2013. Doctoral Dissertation, Reims; Université de Tunis. Faculté des sciences de Tunis. Accessed August 24, 2019. http://www.theses.fr/2013REIMS035.

MLA Handbook (7^th Edition):

Hatmi, Saloua. “Impact du déficit hydrique sur les réponses de défense et la sensibilité de la vigne à Botrytis cinerea : rôle de la dégradation des polyamines. : Impact of water deficiency on defense responses and susceptibility of grapevine to Botrytis cinerea : Role of polyamine oxidation.” 2013. Web. 24 Aug 2019.

Vancouver:

Hatmi S. Impact du déficit hydrique sur les réponses de défense et la sensibilité de la vigne à Botrytis cinerea : rôle de la dégradation des polyamines. : Impact of water deficiency on defense responses and susceptibility of grapevine to Botrytis cinerea : Role of polyamine oxidation. [Internet] [Doctoral dissertation]. Reims; Université de Tunis. Faculté des sciences de Tunis; 2013. [cited 2019 Aug 24]. Available from: http://www.theses.fr/2013REIMS035.

Council of Science Editors:

Hatmi S. Impact du déficit hydrique sur les réponses de défense et la sensibilité de la vigne à Botrytis cinerea : rôle de la dégradation des polyamines. : Impact of water deficiency on defense responses and susceptibility of grapevine to Botrytis cinerea : Role of polyamine oxidation. [Doctoral Dissertation]. Reims; Université de Tunis. Faculté des sciences de Tunis; 2013. Available from: http://www.theses.fr/2013REIMS035

Virginia Tech

23. Szumanski, Maria B. W. Characterization of structure, function and regulation of the speB gene in Escherichia coli.

Degree: PhD, Biochemistry and Nutrition, 1989, Virginia Tech

URL: http://hdl.handle.net/10919/54438

Subjects/Keywords: LD5655.V856 1989.S987; Polyamines; Polyamines in the body

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Szumanski, M. B. W. (1989). Characterization of structure, function and regulation of the speB gene in Escherichia coli. (Doctoral Dissertation). Virginia Tech. Retrieved from http://hdl.handle.net/10919/54438

Chicago Manual of Style (16^th Edition):

Szumanski, Maria B W. “Characterization of structure, function and regulation of the speB gene in Escherichia coli.” 1989. Doctoral Dissertation, Virginia Tech. Accessed August 24, 2019. http://hdl.handle.net/10919/54438.

MLA Handbook (7^th Edition):

Szumanski, Maria B W. “Characterization of structure, function and regulation of the speB gene in Escherichia coli.” 1989. Web. 24 Aug 2019.

Vancouver:

Szumanski MBW. Characterization of structure, function and regulation of the speB gene in Escherichia coli. [Internet] [Doctoral dissertation]. Virginia Tech; 1989. [cited 2019 Aug 24]. Available from: http://hdl.handle.net/10919/54438.

Council of Science Editors:

Szumanski MBW. Characterization of structure, function and regulation of the speB gene in Escherichia coli. [Doctoral Dissertation]. Virginia Tech; 1989. Available from: http://hdl.handle.net/10919/54438

24. Carriel Lopez, Diego. Etude sur la relation fonction-structure de la lysine décarboxylase de Pseudomonas aeruginosa : Structure-function relationships of the lysine decarboxylase from Pseudomonas aeruginosa.

Degree: Docteur es, Biologie Structurale et Nanobiologie, 2017, Grenoble Alpes

URL: http://www.theses.fr/2017GREAV011

► La lysine décarboxylase (LDC) appartient à une famille d'enzymes décamériques dépendantes du cofacteur PLP qui sont connus pour catalyser la réaction transformant la L-Lysine en… (more)

▼ La lysine décarboxylase (LDC) appartient à une famille d'enzymes décamériques dépendantes du cofacteur PLP qui sont connus pour catalyser la réaction transformant la L-Lysine en cadavérine tout en consommant un proton. Dans les entérobactéries comme Escherichia coli, nous trouvons deux paralogues, LdcI et LdcC. LdcI permet de faire face à la bactérie au conditions hostile de pH acide lors du passage à travers du tract gastro-intestinale. LdcC est produite pendant la phase stationnaire et aussi quand les bactéries font face aux traitements antibiotiques. La cadavérine produite par les LDCs est connue pour protéger les bactéries du stress oxydant. Cela s’explique par le fait que la cadavérine bloque les porines de la membrane externe, réduisant ainsi la perméabilité des molécules responsables du stress acides et oxydant. L'activité des LDCs chez E. coli est coordonnée avec la réponse stringente qui est mise en place lorsque les microorganismes sont dans des conditions pauvres en nutriments, afin d’éviter l’épuisement intracellulaire de la L-Lysine nécessaire pour la synthèse des protéines. Cependant, cette inhibition peut être levée par la formation d'un complexe en forme de cage avec son partenaire RavA, permettant ainsi aux bactéries de faire face aux stress multiples. Etant donné que la réponse au stress est importante pour que les bactéries puissent exhiber leur pathogénicité, nous nous sommes demandés si la bactérie opportuniste Pseudomonas aeruginosa pourrait employer LdcA pour contrer des conditions de stress qui ont déjà été décrites pour LdcI chez les entérobactéries. Au cours de ma thèse, nous avons abordé cette question en utilisant différentes approches complémentaires. Tout d'abord, nous avons utilisé des fusions promoteur-gène et de l'analyse par Western-blot pour déterminer les conditions dans lesquelles le gène ldcA a été exprimé et sa protéine synthétisée. Nous avons pu observer que ldcA est exprimé sur la phase stationnaire de croissance dans des conditions aérobies en milieux riches et également pendant des conditions anaérobies de respiration avec nitrate. Nous avons également confirmé que l'expression de ldcA est régulée par ArgR et elle est induite complètement lorsque l’acide aminé L-arginine est présente dans le milieu de croissance. Même si nous avons trouvé que les conditions de stress n'induisent pas l'expression de ldcA, nous avons obtenu de nouvelles données suggérant que d'autres mécanismes de régulation tels que le système de quorum sensing dépendant des quinolones (PQS) pourraient être impliqués dans l'expression de ldcA. En utilisant des souches mutantes de ldcA et son complémentée, nous avons évalué si LdcA était impliqué dans la réponse au stress acide et oxydatif. Bien que les données obtenues à l'aide des expériences dans notre laboratoire et des technologies à haut débit (Biolog) aient révélé que LdcA ne présente pas les mêmes fonctions que LdcI, nous avons découvert que la cadavérine produite par LdcA est nécessaire pour la croissance en milieu minimal avec L- Glutamate comme… Advisors/Committee Members: Gutsche, Irina (thesis director), Elsen, Sylvie (thesis director).

Subjects/Keywords: Physiologie bactérienne; Lysine Decarboxylase; Pseudomonas; E.coli; Polyamines; Bacterial Physiology; Lysine Decarboxylase; Pseudomonas; E. coli; Polyamines; 570

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Carriel Lopez, D. (2017). Etude sur la relation fonction-structure de la lysine décarboxylase de Pseudomonas aeruginosa : Structure-function relationships of the lysine decarboxylase from Pseudomonas aeruginosa. (Doctoral Dissertation). Grenoble Alpes. Retrieved from http://www.theses.fr/2017GREAV011

Chicago Manual of Style (16^th Edition):

Carriel Lopez, Diego. “Etude sur la relation fonction-structure de la lysine décarboxylase de Pseudomonas aeruginosa : Structure-function relationships of the lysine decarboxylase from Pseudomonas aeruginosa.” 2017. Doctoral Dissertation, Grenoble Alpes. Accessed August 24, 2019. http://www.theses.fr/2017GREAV011.

MLA Handbook (7^th Edition):

Carriel Lopez, Diego. “Etude sur la relation fonction-structure de la lysine décarboxylase de Pseudomonas aeruginosa : Structure-function relationships of the lysine decarboxylase from Pseudomonas aeruginosa.” 2017. Web. 24 Aug 2019.

Vancouver:

Carriel Lopez D. Etude sur la relation fonction-structure de la lysine décarboxylase de Pseudomonas aeruginosa : Structure-function relationships of the lysine decarboxylase from Pseudomonas aeruginosa. [Internet] [Doctoral dissertation]. Grenoble Alpes; 2017. [cited 2019 Aug 24]. Available from: http://www.theses.fr/2017GREAV011.

Council of Science Editors:

Carriel Lopez D. Etude sur la relation fonction-structure de la lysine décarboxylase de Pseudomonas aeruginosa : Structure-function relationships of the lysine decarboxylase from Pseudomonas aeruginosa. [Doctoral Dissertation]. Grenoble Alpes; 2017. Available from: http://www.theses.fr/2017GREAV011

The Ohio State University

25. Kim, Byeong Gee. Polyamine metabolism and its relationship to mutiplication and differentiation of acanthamoeba castellanii.

Degree: PhD, Graduate School, 1986, The Ohio State University

URL: http://rave.ohiolink.edu/etdc/view?acc_num=osu1487322984315806

Subjects/Keywords: Biology; Acanthamoeba castellanii; Polyamines

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Kim, B. G. (1986). Polyamine metabolism and its relationship to mutiplication and differentiation of acanthamoeba castellanii. (Doctoral Dissertation). The Ohio State University. Retrieved from http://rave.ohiolink.edu/etdc/view?acc_num=osu1487322984315806

Chicago Manual of Style (16^th Edition):

Kim, Byeong Gee. “Polyamine metabolism and its relationship to mutiplication and differentiation of acanthamoeba castellanii.” 1986. Doctoral Dissertation, The Ohio State University. Accessed August 24, 2019. http://rave.ohiolink.edu/etdc/view?acc_num=osu1487322984315806.

MLA Handbook (7^th Edition):

Kim, Byeong Gee. “Polyamine metabolism and its relationship to mutiplication and differentiation of acanthamoeba castellanii.” 1986. Web. 24 Aug 2019.

Vancouver:

Kim BG. Polyamine metabolism and its relationship to mutiplication and differentiation of acanthamoeba castellanii. [Internet] [Doctoral dissertation]. The Ohio State University; 1986. [cited 2019 Aug 24]. Available from: http://rave.ohiolink.edu/etdc/view?acc_num=osu1487322984315806.

Council of Science Editors:

Kim BG. Polyamine metabolism and its relationship to mutiplication and differentiation of acanthamoeba castellanii. [Doctoral Dissertation]. The Ohio State University; 1986. Available from: http://rave.ohiolink.edu/etdc/view?acc_num=osu1487322984315806

Bowling Green State University

26. Patel, Jigarkumar J. The Role of Polyamine Uptake Transporters on Growth and Development of Arabidopsis Thaliana.

Degree: PhD, Biological Sciences, 2015, Bowling Green State University

URL: http://rave.ohiolink.edu/etdc/view?acc_num=bgsu1429280174

► Transgenic manipulation of polyamine levels has provided compelling evidence that polyamines enable plants to respond to environmental cues by activation of stress and developmental pathways.… (more)

Subjects/Keywords: Biology; Plant Biology; Polyamines; Polyamine Transporters; Putrescine biosynthesis

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APA (6^th Edition):

Patel, J. J. (2015). The Role of Polyamine Uptake Transporters on Growth and Development of Arabidopsis Thaliana. (Doctoral Dissertation). Bowling Green State University. Retrieved from http://rave.ohiolink.edu/etdc/view?acc_num=bgsu1429280174

Chicago Manual of Style (16^th Edition):

Patel, Jigarkumar J. “The Role of Polyamine Uptake Transporters on Growth and Development of Arabidopsis Thaliana.” 2015. Doctoral Dissertation, Bowling Green State University. Accessed August 24, 2019. http://rave.ohiolink.edu/etdc/view?acc_num=bgsu1429280174.

MLA Handbook (7^th Edition):

Patel, Jigarkumar J. “The Role of Polyamine Uptake Transporters on Growth and Development of Arabidopsis Thaliana.” 2015. Web. 24 Aug 2019.

Vancouver:

Patel JJ. The Role of Polyamine Uptake Transporters on Growth and Development of Arabidopsis Thaliana. [Internet] [Doctoral dissertation]. Bowling Green State University; 2015. [cited 2019 Aug 24]. Available from: http://rave.ohiolink.edu/etdc/view?acc_num=bgsu1429280174.

Council of Science Editors:

Patel JJ. The Role of Polyamine Uptake Transporters on Growth and Development of Arabidopsis Thaliana. [Doctoral Dissertation]. Bowling Green State University; 2015. Available from: http://rave.ohiolink.edu/etdc/view?acc_num=bgsu1429280174

27. Sampaio, Valdomiro de Freitas. Efeitos da desnutrição protéica precoce e da estimulação ambiental sobre parâmetros bioquímicos e comportamentais em ratos.

Degree: PhD, Psicobiologia, 2009, University of São Paulo

URL: http://www.teses.usp.br/teses/disponiveis/59/59134/tde-27082009-120622/ ;

►

O presente estudo investigou os efeitos da desnutrição protéica e estimulação ambiental sobre parâmetros bioquímicos em ratos. Foram quantificadas as concentrações de poliaminas no córtex… (more)

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O presente estudo investigou os efeitos da desnutrição protéica e estimulação ambiental sobre parâmetros bioquímicos em ratos. Foram quantificadas as concentrações de poliaminas no córtex frontal, estriado e hipocampo, corticosterona plasmática e atividade da sintase do óxido nítrico no hipocampo. O comportamento exploratório foi analisado no labirinto em cruz elevado (LCE). Os animais receberam dietas contendo 16% (controles - C) ou 6% (desnutridos - D) de proteína e foram divididos em grupos estimulados ou não estimulados. A desnutrição elevou o nível de corticosterona circulante, diminuiu o conteúdo protéico plasmático e diminuiu a ansiedade. Os ratos não estimulados testados no LCE apresentaram nível elevado de corticosterona circulante, diminuída atividade da NOS no hipocampo, menor conteúdo protéico e de poliaminas no córtex frontal, estriado e hipocampo. A estimulação diminuiu o número de entradas nos braços abertos no LCE nos animais do grupo D e aumentou o número de entradas nos braços fechados nos animais do grupo C. A estimulação aumentou a frequência e tempo gasto em comportamentos de avaliação de risco. Estes resultados sugerem que ambos, a desnutrição e o teste no LCE são situações estressantes, como indicado pela elevação dos níveis de corticosterona. Estes resultados são consistentes com menor ansiedade e/ou maior impulsividade nos animais do grupo D.

The present study investigated the effects of protein malnutrition and environmental stimulation on the biochemical and behavioral parameters in rats. It was measured the polyamines concentration in frontal cortex, striatum and hippocampus, as well as the plasma corticosterone levels. The exploratory behavior was analyzed using the elevated plus-maze (EPM) test. Rats received either 16% (well-nourished - W) or 6% (malnourished - M) protein diets and were divided into stimulated or non-stimulated groups. Malnutrition increased corticosterone levels, decreased plasma protein content and anxiety. Non-stimulated rats tested in the EPM increased corticosterone levels, decreased hippocampal NOS activity, decreased frontal cortex, striatum and hippocampus protein and polyamines contents. Stimulation decreased open arm entries in the EPM in M animals and increased the closed arm entries in the W ones. Stimulation increased frequency and time spent in risk-assessment behaviors. These results suggest that both malnutrition and EPM testing are stressing situations as indicated by increased corticosterone levels. These results are consistent with lower anxiety and/or higher impulsiveness in M animals.

Advisors/Committee Members: Almeida, Sebastião de Sousa.

Subjects/Keywords: Ansiedade; Anxiety; Corticosterona; Corticosterone; Desnutrição; Malnutrition; Poliaminas; Polyamines

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APA (6^th Edition):

Sampaio, V. d. F. (2009). Efeitos da desnutrição protéica precoce e da estimulação ambiental sobre parâmetros bioquímicos e comportamentais em ratos. (Doctoral Dissertation). University of São Paulo. Retrieved from http://www.teses.usp.br/teses/disponiveis/59/59134/tde-27082009-120622/ ;

Chicago Manual of Style (16^th Edition):

Sampaio, Valdomiro de Freitas. “Efeitos da desnutrição protéica precoce e da estimulação ambiental sobre parâmetros bioquímicos e comportamentais em ratos.” 2009. Doctoral Dissertation, University of São Paulo. Accessed August 24, 2019. http://www.teses.usp.br/teses/disponiveis/59/59134/tde-27082009-120622/ ;.

MLA Handbook (7^th Edition):

Sampaio, Valdomiro de Freitas. “Efeitos da desnutrição protéica precoce e da estimulação ambiental sobre parâmetros bioquímicos e comportamentais em ratos.” 2009. Web. 24 Aug 2019.

Vancouver:

Sampaio VdF. Efeitos da desnutrição protéica precoce e da estimulação ambiental sobre parâmetros bioquímicos e comportamentais em ratos. [Internet] [Doctoral dissertation]. University of São Paulo; 2009. [cited 2019 Aug 24]. Available from: http://www.teses.usp.br/teses/disponiveis/59/59134/tde-27082009-120622/ ;.

Council of Science Editors:

Sampaio VdF. Efeitos da desnutrição protéica precoce e da estimulação ambiental sobre parâmetros bioquímicos e comportamentais em ratos. [Doctoral Dissertation]. University of São Paulo; 2009. Available from: http://www.teses.usp.br/teses/disponiveis/59/59134/tde-27082009-120622/ ;

Temple University

28. Moncada Benavides, Camilo Andres. EFFECT OF NICOTINE ON LUNG S-ADENOSYLMETHIONINE AND PNEUMOCYSTIS PNEUMONIA DEVELOPMENT.

Degree: PhD, 2012, Temple University

URL: http://digital.library.temple.edu/u?/p245801coll10,206623

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Biochemistry

Infection with "Pneumocystis" causes a ≥ 99% depletion of plasma S-adenosylmethionine (AdoMet) levels in both "Pneumocystis" pneumonia (PcP) animal models and patients. AdoMet is… (more)

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Biochemistry

Infection with "Pneumocystis" causes a ≥ 99% depletion of plasma S-adenosylmethionine (AdoMet) levels in both "Pneumocystis" pneumonia (PcP) animal models and patients. AdoMet is a critical cellular metabolic intermediate, with a pivotal role as methyl donor in a myriad of biochemical processes and necessary for the synthesis of the essential polyamines spermidine and spermine. In the target tissue of "Pneumocystis", the lung, levels of AdoMet were previously shown to be depleted experimentally using nicotine. Here we show that chronic administration of nicotine in an animal model of PcP resulted in decreased lung AdoMet content. Since "Pneumocystis" is dependent on this metabolite, PcP burden was also relived. We hypothesized that the underlying mechanism behind nicotine-induced AdoMet depletion was an increased consumption of AdoMet through the polyamine pathway where the increased activity of N-1-spermidine/spermine acetyl transferase raises the catabolic / anabolic cycling of polyamines, a process that utilizes AdoMet. In a critical test of our hypothesis, we found that blockage of polyamine metabolism via inhibition of the polyamine biosynthetic enzyme ornithine decarboxylase (ODC) hinders the effect of nicotine on lung AdoMet levels. Further support is provided by metabolite analyses showing nicotine to cause a strong diversion of AdoMet toward polyamine synthesis and away from methylation reactions; these shifts are also reversed by inhibition of ODC. Because the nicotine effect on "Pneumocystis" is so striking, we considered the possibility of tissue specificity. Using laser capture microdissection (LCM), we collected samples of lung alveolar regions (site of infection) and respiratory epithelium for controls. We found nicotine to cause increased ODC activity in alveolar regions but not airway epithelium; we conclude that tissue specificity likely contributes to the effect of nicotine on "Pneumocystis" pneumonia. Our studies demonstrate the feasibility of pharmacological manipulation of the polyamine pathway in order to reduce AdoMet levels in the lung and prompted the assessment of compounds alternative to nicotine with the potential to achieve a comparable effect. In vitro evaluation of the polyamine analog DENSPM along with putrescine in type II alveolar cell lines, indicates that although such a combination has the potential to induce polyamine flux, an apparent competition for the same polyamine transport system impairs simultaneous uptake of both compounds at effective concentrations. In conclusion, we showed that chronic nicotine administration causes reduction of AdoMet levels in rat lung following 21 days of treatment, by a mechanism involving the induction of polyamine flux, which is responsible of increased AdoMet utilization for polyamine biosynthesis. According to LCM-based analysis, this effect seems to be confined to the alveolar regions of the lung.

Temple University – Theses

Advisors/Committee Members: Merali, Salim, Chong, Parkson Lee-Gau, Soprano, Dianne R., Collins, Jimmy H., Yarlett, Nigel.

Subjects/Keywords: Biochemistry; Laser Capture Microdissection; Lung; Nicotine; Pneumocystis; Polyamines; S-Adenosylmethionine

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APA (6^th Edition):

Moncada Benavides, C. A. (2012). EFFECT OF NICOTINE ON LUNG S-ADENOSYLMETHIONINE AND PNEUMOCYSTIS PNEUMONIA DEVELOPMENT. (Doctoral Dissertation). Temple University. Retrieved from http://digital.library.temple.edu/u?/p245801coll10,206623

Chicago Manual of Style (16^th Edition):

Moncada Benavides, Camilo Andres. “EFFECT OF NICOTINE ON LUNG S-ADENOSYLMETHIONINE AND PNEUMOCYSTIS PNEUMONIA DEVELOPMENT.” 2012. Doctoral Dissertation, Temple University. Accessed August 24, 2019. http://digital.library.temple.edu/u?/p245801coll10,206623.

MLA Handbook (7^th Edition):

Moncada Benavides, Camilo Andres. “EFFECT OF NICOTINE ON LUNG S-ADENOSYLMETHIONINE AND PNEUMOCYSTIS PNEUMONIA DEVELOPMENT.” 2012. Web. 24 Aug 2019.

Vancouver:

Moncada Benavides CA. EFFECT OF NICOTINE ON LUNG S-ADENOSYLMETHIONINE AND PNEUMOCYSTIS PNEUMONIA DEVELOPMENT. [Internet] [Doctoral dissertation]. Temple University; 2012. [cited 2019 Aug 24]. Available from: http://digital.library.temple.edu/u?/p245801coll10,206623.

Council of Science Editors:

Moncada Benavides CA. EFFECT OF NICOTINE ON LUNG S-ADENOSYLMETHIONINE AND PNEUMOCYSTIS PNEUMONIA DEVELOPMENT. [Doctoral Dissertation]. Temple University; 2012. Available from: http://digital.library.temple.edu/u?/p245801coll10,206623

University of Alberta

29. Laplante, Carmen M. Site-selective N-methylation of resin bound terminal amines and borane reduction/iodine work-up: amino acid side chain compatibility and solution studies.

Degree: MS, Department of Chemistry, 2001, University of Alberta

URL: https://era.library.ualberta.ca/files/xs55mf16d

Subjects/Keywords: Oxidation-reduction reaction.; Polyamines.; Methylation.

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APA (6^th Edition):

Laplante, C. M. (2001). Site-selective N-methylation of resin bound terminal amines and borane reduction/iodine work-up: amino acid side chain compatibility and solution studies. (Masters Thesis). University of Alberta. Retrieved from https://era.library.ualberta.ca/files/xs55mf16d

Chicago Manual of Style (16^th Edition):

Laplante, Carmen M. “Site-selective N-methylation of resin bound terminal amines and borane reduction/iodine work-up: amino acid side chain compatibility and solution studies.” 2001. Masters Thesis, University of Alberta. Accessed August 24, 2019. https://era.library.ualberta.ca/files/xs55mf16d.

MLA Handbook (7^th Edition):

Laplante, Carmen M. “Site-selective N-methylation of resin bound terminal amines and borane reduction/iodine work-up: amino acid side chain compatibility and solution studies.” 2001. Web. 24 Aug 2019.

Vancouver:

Laplante CM. Site-selective N-methylation of resin bound terminal amines and borane reduction/iodine work-up: amino acid side chain compatibility and solution studies. [Internet] [Masters thesis]. University of Alberta; 2001. [cited 2019 Aug 24]. Available from: https://era.library.ualberta.ca/files/xs55mf16d.

Council of Science Editors:

Laplante CM. Site-selective N-methylation of resin bound terminal amines and borane reduction/iodine work-up: amino acid side chain compatibility and solution studies. [Masters Thesis]. University of Alberta; 2001. Available from: https://era.library.ualberta.ca/files/xs55mf16d

University of Pretoria

30. Niemand, Jandeli. Biochemical characterisation of putrescine and spermidine uptake as a potential therapeutic target against the human malaria parasite, Plasmodium falciparum.

Degree: Biochemistry, 2012, University of Pretoria

URL: http://hdl.handle.net/2263/24986

► Plasmodium falciparum causes the most severe form of human malaria, and the continual development of resistance of this parasite to current anti-malarial drugs underpins a… (more)

▼ Plasmodium falciparum causes the most severe form of human malaria, and the continual development of resistance of this parasite to current anti-malarial drugs underpins a pressing need for the discovery of novel chemotherapeutic approaches. Polyamines and their biosynthetic enzymes are present at high levels in rapidly proliferating cells, including cancer cells and protozoan parasites. Inhibition of the malaria parasite’s polyamine biosynthesis pathway causes cytostatic arrest in the trophozoite stage, but does not cure infections in vivo. This may be due to the salvage of exogenous polyamines from the host, replenishing the intracellular polyamine pool; however the mechanism(s) of polyamine uptake by the intraerythrocytic parasite are not well understood. In this study the uptake of the polyamines putrescine and spermidine into P. falciparum-infected erythrocytes (iRBC) well as into P. falciparum parasites functionally isolated from their host cell by saponin-permeabilisation of the erythrocyte membrane was investigated using radioisotope flux techniques. While the characteristics of transport of putrescine into infected erythrocytes were similar to those of transport into uninfected erythrocytes, spermidine entered iRBC in part via the ‘new permeation pathways’ induced by the parasite in the erythrocyte membrane. Both putrescine and spermidine were taken up across the plasma membrane of isolated parasites via a saturable, temperature-dependent process that showed competition between different polyamines as well as the polyamine precursor ornithine and basic amino acids. Inhibition of polyamine biosynthesis led to increased total uptake of both putrescine and spermidine. The influx of putrescine and spermidine into isolated parasites was independent of Na+ but increased with increasing pH and showed a marked dependence on the membrane potential, decreasing with membrane depolarisation and increasing with membrane hyperpolarisation. Both anthracene and polyamine derivatives have been shown to have anti-malarial activity. Anthracene-polyamine conjugates have been developed with the aim of utilising the polyamine uptake mechanisms of cancer cells to deliver the cytotoxic anthracene moieties to these cells. Here, several anthracene-polyamine conjugates showed promising anti-malarial activity. These compounds inhibited parasite proliferation with IC50 values in the nM range, and caused an arrest in the cell cycle, as well as a decrease in the mitochondrial membrane potential. Cytotoxicity could not be reversed by the addition of exogenous polyamines, nor did the conjugates have an effect on intracellular polyamine levels. This doctoral study showed that P. falciparum parasites not only synthesise polyamines, but can also acquire putrescine and spermidine from the extracellular environment and paves the way for interfering with polyamine metabolism as an anti-parasitic strategy. Advisors/Committee Members: Birkholtz, Lyn-Marie (advisor), Louw, Abraham Izak (advisor).

Subjects/Keywords: Human malaria parasite; Plasmodium falciparum; Biosynthetic enzymes; Polyamines; UCTD

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Niemand, J. (2012). Biochemical characterisation of putrescine and spermidine uptake as a potential therapeutic target against the human malaria parasite, Plasmodium falciparum. (Doctoral Dissertation). University of Pretoria. Retrieved from http://hdl.handle.net/2263/24986

Chicago Manual of Style (16^th Edition):

Niemand, Jandeli. “Biochemical characterisation of putrescine and spermidine uptake as a potential therapeutic target against the human malaria parasite, Plasmodium falciparum.” 2012. Doctoral Dissertation, University of Pretoria. Accessed August 24, 2019. http://hdl.handle.net/2263/24986.

MLA Handbook (7^th Edition):

Niemand, Jandeli. “Biochemical characterisation of putrescine and spermidine uptake as a potential therapeutic target against the human malaria parasite, Plasmodium falciparum.” 2012. Web. 24 Aug 2019.

Vancouver:

Niemand J. Biochemical characterisation of putrescine and spermidine uptake as a potential therapeutic target against the human malaria parasite, Plasmodium falciparum. [Internet] [Doctoral dissertation]. University of Pretoria; 2012. [cited 2019 Aug 24]. Available from: http://hdl.handle.net/2263/24986.

Council of Science Editors:

Niemand J. Biochemical characterisation of putrescine and spermidine uptake as a potential therapeutic target against the human malaria parasite, Plasmodium falciparum. [Doctoral Dissertation]. University of Pretoria; 2012. Available from: http://hdl.handle.net/2263/24986

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Open Access Theses and Dissertations