subject:(polyamines)

University of Guelph

1. Krause, Michael. Sterically Demanding Ethylenediamines and Polyamines for the Stabilization of Main Group Elements in Low Coordination Numbers.

Degree: MS, Department of Chemistry, 2011, University of Guelph

URL: https://atrium.lib.uoguelph.ca/xmlui/handle/10214/3148

► This thesis explores the reaction of primary amines with 1,2-dibromoethane and 1,3-dibromopropane as a one step synthesis of N,N"-disubstituted ethylenediamines, RNH- CH2CH2-NH-R, N,N"-disubstituted propanediamines, R-NH-CH2CH2CH2-NH-R… (more)

Subjects/Keywords: ethylenediamines; polyamines

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APA (6^th Edition):

Krause, M. (2011). Sterically Demanding Ethylenediamines and Polyamines for the Stabilization of Main Group Elements in Low Coordination Numbers. (Masters Thesis). University of Guelph. Retrieved from https://atrium.lib.uoguelph.ca/xmlui/handle/10214/3148

Chicago Manual of Style (16^th Edition):

Krause, Michael. “Sterically Demanding Ethylenediamines and Polyamines for the Stabilization of Main Group Elements in Low Coordination Numbers.” 2011. Masters Thesis, University of Guelph. Accessed March 05, 2021. https://atrium.lib.uoguelph.ca/xmlui/handle/10214/3148.

MLA Handbook (7^th Edition):

Krause, Michael. “Sterically Demanding Ethylenediamines and Polyamines for the Stabilization of Main Group Elements in Low Coordination Numbers.” 2011. Web. 05 Mar 2021.

Vancouver:

Krause M. Sterically Demanding Ethylenediamines and Polyamines for the Stabilization of Main Group Elements in Low Coordination Numbers. [Internet] [Masters thesis]. University of Guelph; 2011. [cited 2021 Mar 05]. Available from: https://atrium.lib.uoguelph.ca/xmlui/handle/10214/3148.

Council of Science Editors:

Krause M. Sterically Demanding Ethylenediamines and Polyamines for the Stabilization of Main Group Elements in Low Coordination Numbers. [Masters Thesis]. University of Guelph; 2011. Available from: https://atrium.lib.uoguelph.ca/xmlui/handle/10214/3148

Oregon State University

2. Rathi, Mahesh Shrikant. Application of polyallylamine as a dry strength agent.

Degree: MS, Forest Products, 1999, Oregon State University

URL: http://hdl.handle.net/1957/18414

► Dry strength is an inherent structural property of a paper sheet. It is due primarily to the development of fiber-to-fiber bonds. Polyamines, especially polyallylamine·HC1 (PAA),… (more)

Subjects/Keywords: Polyamines

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APA (6^th Edition):

Rathi, M. S. (1999). Application of polyallylamine as a dry strength agent. (Masters Thesis). Oregon State University. Retrieved from http://hdl.handle.net/1957/18414

Chicago Manual of Style (16^th Edition):

Rathi, Mahesh Shrikant. “Application of polyallylamine as a dry strength agent.” 1999. Masters Thesis, Oregon State University. Accessed March 05, 2021. http://hdl.handle.net/1957/18414.

MLA Handbook (7^th Edition):

Rathi, Mahesh Shrikant. “Application of polyallylamine as a dry strength agent.” 1999. Web. 05 Mar 2021.

Vancouver:

Rathi MS. Application of polyallylamine as a dry strength agent. [Internet] [Masters thesis]. Oregon State University; 1999. [cited 2021 Mar 05]. Available from: http://hdl.handle.net/1957/18414.

Council of Science Editors:

Rathi MS. Application of polyallylamine as a dry strength agent. [Masters Thesis]. Oregon State University; 1999. Available from: http://hdl.handle.net/1957/18414

Loyola University Chicago

3. Dial, Courtney Noelle. Modulation of Coxsackievirus Protease Activity by Polyamines.

Degree: MS, Microbiology and Immunology, 2019, Loyola University Chicago

URL: https://ecommons.luc.edu/luc_theses/3985

► Coxsackievirus type B (CVB3) is one of the six serotypes of the Coxsackievirus family of non-enveloped, linear, and positive-sense single-stranded RNA viruses. It is a… (more)

▼ Coxsackievirus type B (CVB3) is one of the six serotypes of the Coxsackievirus family of non-enveloped, linear, and positive-sense single-stranded RNA viruses. It is a pathogenic enterovirus that belongs to the same genus as the notable pathogen poliovirus. CVB3 can cause a range of illnesses from a fever to gastrointestinal distress but is most noteworthy for the ability to cause viral myocarditis, a swelling of the heart muscle. Coxsackievirus, like all RNA viruses, tends to develop mutations rapidly due to its error prone polymerase and lack of proofreading activity. These mutations can be advantageous for the virus, allowing it to develop traits that enhance replication and pathogenesis. Due to its major clinical importance and a lack of available vaccine and antivirals, there is an impetus to identify effective antivirals against CVB3. In that regard, the virus' ability to mutate poses a major obstacle to successful antiviral treatment and must be explored further to better understand the mechanisms of antiviral resistance and improve drug development.Polyamines are small positively charged molecules that are present in all cells and have crucial roles in processes such as transcription, translation, DNA replication and signaling. They are also fundamental to a virus' ability to infect a host. When drugs that diminish polyamines are added to cells, the virus' ability to replicate tapers. One drug that acts in this way is difluoromethylornithine (DFMO), which diminishes the production of polyamines by inhibiting the first step in polyamine synthesis: the conversion of ornithine to putrescine. In order to better understand how CVB3 could evolve resistance to antiviral treatments, CVB3 was seriallypassaged in the presence of DFMO. The resultant mutations that arose in CVB3 may correlate with its ability to infect a host who is undergoing antiviral therapy.Passaging CVB3 in the presence of DFMO illuminated 3 mutations: VP3- 234R, which is a site in the viral capsid; 2A- Q29K and 3C- Q52R both of which are sites in viral proteases. These proteases are crucial for multiple stages of infection including protein processing and packaging. Due to the vital importance of the viral proteases, these mutations and their seeming tie with polyamines, the focus of this study will revolve around these mutations. From these initial findings, we hypothesize that polyamines modulate viral protease activity and CVB3 gains resistance to polyamine depletion via mutation of its protease(s).

Subjects/Keywords: Coxsackievirus B3; Polyamines; Protease; Microbiology

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APA (6^th Edition):

Dial, C. N. (2019). Modulation of Coxsackievirus Protease Activity by Polyamines. (Thesis). Loyola University Chicago. Retrieved from https://ecommons.luc.edu/luc_theses/3985

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Dial, Courtney Noelle. “Modulation of Coxsackievirus Protease Activity by Polyamines.” 2019. Thesis, Loyola University Chicago. Accessed March 05, 2021. https://ecommons.luc.edu/luc_theses/3985.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Dial, Courtney Noelle. “Modulation of Coxsackievirus Protease Activity by Polyamines.” 2019. Web. 05 Mar 2021.

Vancouver:

Dial CN. Modulation of Coxsackievirus Protease Activity by Polyamines. [Internet] [Thesis]. Loyola University Chicago; 2019. [cited 2021 Mar 05]. Available from: https://ecommons.luc.edu/luc_theses/3985.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Dial CN. Modulation of Coxsackievirus Protease Activity by Polyamines. [Thesis]. Loyola University Chicago; 2019. Available from: https://ecommons.luc.edu/luc_theses/3985

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Montana State University

4. Woller, Eric Kevin. Mannose-functionalized PAMAM dendrimers : their synthesis, characterization and use in refining the model of protein-carbohydrate interactions.

Degree: PhD, College of Letters & Science, 2003, Montana State University

URL: https://scholarworks.montana.edu/xmlui/handle/1/8368

Subjects/Keywords: Polyamines.; Glycosides.

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APA (6^th Edition):

Woller, E. K. (2003). Mannose-functionalized PAMAM dendrimers : their synthesis, characterization and use in refining the model of protein-carbohydrate interactions. (Doctoral Dissertation). Montana State University. Retrieved from https://scholarworks.montana.edu/xmlui/handle/1/8368

Chicago Manual of Style (16^th Edition):

Woller, Eric Kevin. “Mannose-functionalized PAMAM dendrimers : their synthesis, characterization and use in refining the model of protein-carbohydrate interactions.” 2003. Doctoral Dissertation, Montana State University. Accessed March 05, 2021. https://scholarworks.montana.edu/xmlui/handle/1/8368.

MLA Handbook (7^th Edition):

Woller, Eric Kevin. “Mannose-functionalized PAMAM dendrimers : their synthesis, characterization and use in refining the model of protein-carbohydrate interactions.” 2003. Web. 05 Mar 2021.

Vancouver:

Woller EK. Mannose-functionalized PAMAM dendrimers : their synthesis, characterization and use in refining the model of protein-carbohydrate interactions. [Internet] [Doctoral dissertation]. Montana State University; 2003. [cited 2021 Mar 05]. Available from: https://scholarworks.montana.edu/xmlui/handle/1/8368.

Council of Science Editors:

Woller EK. Mannose-functionalized PAMAM dendrimers : their synthesis, characterization and use in refining the model of protein-carbohydrate interactions. [Doctoral Dissertation]. Montana State University; 2003. Available from: https://scholarworks.montana.edu/xmlui/handle/1/8368

University of Illinois – Urbana-Champaign

5. Jin, Jing. The potential antioxidant and anticancer properties of polyamines and their response to high-intensity ultrasonication.

Degree: MS, Food Science & Human Nutrition, 2017, University of Illinois – Urbana-Champaign

URL: http://hdl.handle.net/2142/99538

► Polyamines are widespread in living cells and play several important roles in both plants and animals. The polyamines spermine, spermidine and putrescine have been of… (more)

▼ Polyamines are widespread in living cells and play several important roles in both plants and animals. The polyamines spermine, spermidine and putrescine have been of primary interest, as they participate in cell growth and proliferation and thus are involved in health, disease and aging. Polyamines are also reported as secondary metabolites in stress response in plants. However, many of these functions have not been scientifically demonstrated. Thus, the objective of this research was to: (1) compare the antioxidant and anti-carcinogenic capacity of polyamines through various in vitro assays; (2) study the effect of ultrasound on secondary metabolite synthesis (polyamines) and maintenance of lettuce quality. Antioxidant capacity of polyamines was compared using two commonly used antioxidant capacity assays. The inhibitory effect of polyamines on cancer cell growth was investigated using murine breast cancer 4T1 cells and MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay to monitor cell growth. Tyramine had the highest antioxidant capacity, followed by tryptamine, spermine, putrescine, and spermidine. Agmatine showed no antioxidant capacity. Spermine and spermidine inhibited the growth of 4T1 cells at IC50 of 6.0 µg/mL and 21.5 µg/mL, respectively. No anti-carcinogenic effects were observed in this cell system with putrescine. Abiotic stress application was conducted using sonication. Gas chromatographic analysis revealed that immediately after sonication, polyamine concentrations were not significantly different between unsonicated and sonicated lettuce samples. After three days recovery, the unsonicated lettuce leaves displayed significantly higher concentrations of putrescine and spermidine as compared to sonicated leaf tissue. This was also linked with a much higher quality of the lettuce leaves from sonicated tissue. This represents the first report of comparison of different polyamines for antioxidant capacity by two popular methods (ORAC and DPPH). For anti-carcinogenic activity, data were generated from one cell line indicating that the polyamines, but not putrescine, may prevent cell growth. Ultrasonication was found to be efficient in maintaining the quality of lettuce during storage, correspondingly, the polyamine levels in sonicated lettuce were decreased after 72 hours storage compared to control lettuce. Further studies are needed to ascertain the role of polyamines in cancer utilizing other cell lines. Microbial populations in sonicated and unsonicated lettuce after 72 hours recovery can also be tested to strengthen the impact of quality maintenance related to ultrasound treatment. Advisors/Committee Members: Engeseth, Nicki Jene (advisor), Helferich, William (committee member), Feng, Hao (committee member).

Subjects/Keywords: Polyamines; Antioxidant; Anticancer; Ultrasonication; Stress

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APA (6^th Edition):

Jin, J. (2017). The potential antioxidant and anticancer properties of polyamines and their response to high-intensity ultrasonication. (Thesis). University of Illinois – Urbana-Champaign. Retrieved from http://hdl.handle.net/2142/99538

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Jin, Jing. “The potential antioxidant and anticancer properties of polyamines and their response to high-intensity ultrasonication.” 2017. Thesis, University of Illinois – Urbana-Champaign. Accessed March 05, 2021. http://hdl.handle.net/2142/99538.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Jin, Jing. “The potential antioxidant and anticancer properties of polyamines and their response to high-intensity ultrasonication.” 2017. Web. 05 Mar 2021.

Vancouver:

Jin J. The potential antioxidant and anticancer properties of polyamines and their response to high-intensity ultrasonication. [Internet] [Thesis]. University of Illinois – Urbana-Champaign; 2017. [cited 2021 Mar 05]. Available from: http://hdl.handle.net/2142/99538.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Jin J. The potential antioxidant and anticancer properties of polyamines and their response to high-intensity ultrasonication. [Thesis]. University of Illinois – Urbana-Champaign; 2017. Available from: http://hdl.handle.net/2142/99538

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Aberdeen

6. Acheampong, Paul. Polyamines.

Degree: School of Medicine and Dentistry., 2010, University of Aberdeen

URL: http://digitool.abdn.ac.uk:80/webclient/DeliveryManager?application=DIGITOOL-3&owner=resourcediscovery&custom_att_2=simple_viewer&pid=158358 ; http://digitool.abdn.ac.uk:1801/webclient/DeliveryManager?pid=158358&custom_att_2=simple_viewer

Subjects/Keywords: Polyamines.; Stroke.

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APA (6^th Edition):

Acheampong, P. (2010). Polyamines. (Doctoral Dissertation). University of Aberdeen. Retrieved from http://digitool.abdn.ac.uk:80/webclient/DeliveryManager?application=DIGITOOL-3&owner=resourcediscovery&custom_att_2=simple_viewer&pid=158358 ; http://digitool.abdn.ac.uk:1801/webclient/DeliveryManager?pid=158358&custom_att_2=simple_viewer

Chicago Manual of Style (16^th Edition):

Acheampong, Paul. “Polyamines.” 2010. Doctoral Dissertation, University of Aberdeen. Accessed March 05, 2021. http://digitool.abdn.ac.uk:80/webclient/DeliveryManager?application=DIGITOOL-3&owner=resourcediscovery&custom_att_2=simple_viewer&pid=158358 ; http://digitool.abdn.ac.uk:1801/webclient/DeliveryManager?pid=158358&custom_att_2=simple_viewer.

MLA Handbook (7^th Edition):

Acheampong, Paul. “Polyamines.” 2010. Web. 05 Mar 2021.

Vancouver:

Acheampong P. Polyamines. [Internet] [Doctoral dissertation]. University of Aberdeen; 2010. [cited 2021 Mar 05]. Available from: http://digitool.abdn.ac.uk:80/webclient/DeliveryManager?application=DIGITOOL-3&owner=resourcediscovery&custom_att_2=simple_viewer&pid=158358 ; http://digitool.abdn.ac.uk:1801/webclient/DeliveryManager?pid=158358&custom_att_2=simple_viewer.

Council of Science Editors:

Acheampong P. Polyamines. [Doctoral Dissertation]. University of Aberdeen; 2010. Available from: http://digitool.abdn.ac.uk:80/webclient/DeliveryManager?application=DIGITOOL-3&owner=resourcediscovery&custom_att_2=simple_viewer&pid=158358 ; http://digitool.abdn.ac.uk:1801/webclient/DeliveryManager?pid=158358&custom_att_2=simple_viewer

University of Arizona

7. Vargas, Ashley Joy. Assessing the Role of Dietary Polyamines on the Continuum of Colorectal Carcinoma .

Degree: 2013, University of Arizona

URL: http://hdl.handle.net/10150/293416

► Putrescine, spermidine and spermine are the polyamines biosynthesized by human cells via ornithine decarboxylase (ODC) and are also sourced from the diet. Polyamines are required… (more)

▼ Putrescine, spermidine and spermine are the polyamines biosynthesized by human cells via ornithine decarboxylase (ODC) and are also sourced from the diet. Polyamines are required for malignant and normal cell growth and development. Pharmacological suppression of polyamine biosynthesis, by difluoromethylornithine, and inflammation, via sulindac, has demonstrated ~70% efficacy in preventing premalignant colorectal adenomas (CRA) in a clinical trial; however, high polyamine intakes mitigated this preventative action. Further, dietary polyamines increase the dysplasia of CRA in initiated animal models of colorectal cancer (CRC) and are hypothesized to function as tumor promoters. Human research on dietary polyamines was limited until the development of a dietary database in 2007 but, continues to be limited by the lack of a biomarker of exposure. Chapter 1 of this dissertation tests the hypothesis that dietary polyamines increase risk of CRA in polyp-formers (n = 1164) and found evidence to support this hypothesis. However, only women, younger participants and certain genotypes experienced more risk of CRA with high polyamine exposure. Chapter II tests the hypothesis that dietary polyamines increase the risk for CRC in an average risk cohort of post-menopausal women (n = 87,620) and did not find evidence to support this hypothesis in the whole population. Rather, dietary polyamines were non-significantly protective against CRC and significantly protective when paired with aspirin use and against CRC-specific death. There was some evidence to support an increase in risk of CRC in younger participants with high polyamine exposure. Overall, the first two chapters suggest that dietary polyamines protect the colorectum in normal risk individuals but promote carcinogenesis in high risk individuals. Chapter III tests the hypothesis that dietary polyamine intake correlates with urinary polyamine output in a group of overweight/obese, older men (n = 36) and Chapter IV tests the hypothesis that intake of highly ripe sweet cherries will increase urinary polyamine output in a subgroup of 10 men from Chapter III. The findings from these chapters suggest there may be a positive correlation, but that a better measure of dietary polyamine intake is needed to determine if urinary polyamines are biomarkers of exposure to polyamines. Advisors/Committee Members: Thompson, Patricia A (advisor), Thompson, Patricia A. (committeemember), Thompson, Cynthia A. (committeemember), Gerner, Eugene W. (committeemember), Romagnolo, Donato F. (committeemember), Schroeder, Joyce A. (committeemember).

Subjects/Keywords: Colorectal cancer; Dietary polyamines; Polyamines; Putrescine; Spermidine; Nutritional Sciences; Colorectal adenoma

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APA (6^th Edition):

Vargas, A. J. (2013). Assessing the Role of Dietary Polyamines on the Continuum of Colorectal Carcinoma . (Doctoral Dissertation). University of Arizona. Retrieved from http://hdl.handle.net/10150/293416

Chicago Manual of Style (16^th Edition):

Vargas, Ashley Joy. “Assessing the Role of Dietary Polyamines on the Continuum of Colorectal Carcinoma .” 2013. Doctoral Dissertation, University of Arizona. Accessed March 05, 2021. http://hdl.handle.net/10150/293416.

MLA Handbook (7^th Edition):

Vargas, Ashley Joy. “Assessing the Role of Dietary Polyamines on the Continuum of Colorectal Carcinoma .” 2013. Web. 05 Mar 2021.

Vancouver:

Vargas AJ. Assessing the Role of Dietary Polyamines on the Continuum of Colorectal Carcinoma . [Internet] [Doctoral dissertation]. University of Arizona; 2013. [cited 2021 Mar 05]. Available from: http://hdl.handle.net/10150/293416.

Council of Science Editors:

Vargas AJ. Assessing the Role of Dietary Polyamines on the Continuum of Colorectal Carcinoma . [Doctoral Dissertation]. University of Arizona; 2013. Available from: http://hdl.handle.net/10150/293416

NSYSU

8. Wang, Kuo-Cheng. Polyamines affect early floral initiation in Doritis pulcherrima and Phalaenopsis.

Degree: Master, Biological Sciences, 2002, NSYSU

URL: http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0711102-163013

► Polyamine titer in the leaves of the self-seedlings of Doritis pulcherrima Lindleyï¼cv. S84-3352ï¼under short-day ( SD, 9 hours of light period ) and long-day (… (more)

Subjects/Keywords: Doritis; polyamines; Phalaenopsis

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APA (6^th Edition):

Wang, K. (2002). Polyamines affect early floral initiation in Doritis pulcherrima and Phalaenopsis. (Thesis). NSYSU. Retrieved from http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0711102-163013

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Wang, Kuo-Cheng. “Polyamines affect early floral initiation in Doritis pulcherrima and Phalaenopsis.” 2002. Thesis, NSYSU. Accessed March 05, 2021. http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0711102-163013.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Wang, Kuo-Cheng. “Polyamines affect early floral initiation in Doritis pulcherrima and Phalaenopsis.” 2002. Web. 05 Mar 2021.

Vancouver:

Wang K. Polyamines affect early floral initiation in Doritis pulcherrima and Phalaenopsis. [Internet] [Thesis]. NSYSU; 2002. [cited 2021 Mar 05]. Available from: http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0711102-163013.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Wang K. Polyamines affect early floral initiation in Doritis pulcherrima and Phalaenopsis. [Thesis]. NSYSU; 2002. Available from: http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0711102-163013

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Pretoria

9. [No author]. Functional genomics analysis of the effects of co-inhibition of the malarial S-adenosylmethionine decarboxylase/ornithine decarboxylase .

Degree: 2009, University of Pretoria

URL: http://upetd.up.ac.za/thesis/available/etd-05302009-124548/

► Polyamines are ubiquitous components of all living cells and their depletion usually causes growth arrest or cytostasis, a strategy employed for treatment of West-African trypanosomiasis.… (more)

▼ Polyamines are ubiquitous components of all living cells and their depletion usually causes growth arrest or cytostasis, a strategy employed for treatment of West-African trypanosomiasis. In the malaria parasite, Plasmodium falciparum, polyamine biosynthesis is regulated by the uniquely bifunctional protein, Sadenosylmethionine decarboxylase/ornithine decarboxylase (PfAdoMetDC/ODC). The unique nature of this protein could provide a selective mechanism for antimalarial treatment. To validate polyamine depletion and specifically PfAdoMetDC/ODC, as drug target for antimalarial therapeutic intervention, polyamine biosynthesis was completely restrained via the inhibition of both catalytic sites of PfAdoMetDC/ODC with DFMO and MDL73811. The physiological effects during the resulting cytostasis were studied with a comprehensive functional genomics approach. The study was preceded by various assays to determine the treatment dosage that would result in complete cytostasis, without non-specific chemical cytotoxicity. The results obtained revealed that the cytostatic mechanism with growth arrest of the treated parasites and normal progression of the untreated controls require special consideration for basic comparisons of response in terms of the assay methodology used and data analysis. This is particularly important when studying a multistage organism such as P. falciparum, which constantly develops and change during the intraerythrocytic developmental cycle, such that growth arrest compared to normal progression would result in significant differences merely due to stage. This critical principle was kept in mind throughout the investigation and was applied to the relative quantification of RNA, proteins and metabolites via a relative time zero approach as opposed to the standard parallel time point comparison. Three independent functional genomics investigations, namely transcriptomics, proteomics and metabolomics were conducted, in which highly synchronised 3D7 parasite cultures were treated during the schizont stage and parasites were sampled during a time course at three time points (just before and during cytostasis). Transcriptome analysis revealed the occurrence of a generalised transcriptional arrest just prior to the growth arrest. To our knowledge this is the first time that transcriptional arrest as the preceding mechanism of cytostasis due to polyamine depletion, was demonstrated. However, despite the transcriptional arrest, the abundance of 538 transcripts was differentially affected and included three perturbation-specific compensatory transcriptional responses: the increased abundance of the transcripts for lysine decarboxylase and ornithine aminotransferase (OAT) and the decreased abundance of that for S-adenosylmethionine synthetase (AdoMet synthetase). Pearson correlations indicated more subtle effects of the perturbation on the proteome and even more so on the metabolome where homeostasis was generally maintained, except downstream to the enzymatic blockade at PfAdoMetDC/ODC. The perturbation-specific… Advisors/Committee Members: Dr L Birkholtz (advisor), Prof A I Louw (advisor), Prof M Llinas (advisor).

Subjects/Keywords: P. falciparum; Malaria parasite; Polyamines; UCTD

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APA (6^th Edition):

author], [. (2009). Functional genomics analysis of the effects of co-inhibition of the malarial S-adenosylmethionine decarboxylase/ornithine decarboxylase . (Doctoral Dissertation). University of Pretoria. Retrieved from http://upetd.up.ac.za/thesis/available/etd-05302009-124548/

Chicago Manual of Style (16^th Edition):

author], [No. “Functional genomics analysis of the effects of co-inhibition of the malarial S-adenosylmethionine decarboxylase/ornithine decarboxylase .” 2009. Doctoral Dissertation, University of Pretoria. Accessed March 05, 2021. http://upetd.up.ac.za/thesis/available/etd-05302009-124548/.

MLA Handbook (7^th Edition):

author], [No. “Functional genomics analysis of the effects of co-inhibition of the malarial S-adenosylmethionine decarboxylase/ornithine decarboxylase .” 2009. Web. 05 Mar 2021.

Vancouver:

author] [. Functional genomics analysis of the effects of co-inhibition of the malarial S-adenosylmethionine decarboxylase/ornithine decarboxylase . [Internet] [Doctoral dissertation]. University of Pretoria; 2009. [cited 2021 Mar 05]. Available from: http://upetd.up.ac.za/thesis/available/etd-05302009-124548/.

Council of Science Editors:

author] [. Functional genomics analysis of the effects of co-inhibition of the malarial S-adenosylmethionine decarboxylase/ornithine decarboxylase . [Doctoral Dissertation]. University of Pretoria; 2009. Available from: http://upetd.up.ac.za/thesis/available/etd-05302009-124548/

10. Κουρνούτου, Γεωργία. Μεταβολές στα επίπεδα των πολυαμινών του πεπτικού αδένα του μυδιού Mytilus galloprovincialis υπό την επίδραση οξειδωτικού στρες.

Degree: 2013, University of Patras

URL: http://hdl.handle.net/10889/6237

►

Οι πολυαμίνες είναι μικρά κατιοντικά μόρια απαραίτητα για την ανάπτυξη και τη διαφοροποίηση των κυττάρων. Εμπλέκονται σε ένα μεγάλο αριθμό κυτταρικών διεργασιών και παίζουν σημαντικό… (more)

Subjects/Keywords: Πολυαμίνες; Οξειδωτικό στρες; 572.548; Polyamines; Oxidative stress

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APA (6^th Edition):

Κουρνούτου, �. (2013). Μεταβολές στα επίπεδα των πολυαμινών του πεπτικού αδένα του μυδιού Mytilus galloprovincialis υπό την επίδραση οξειδωτικού στρες. (Masters Thesis). University of Patras. Retrieved from http://hdl.handle.net/10889/6237

Chicago Manual of Style (16^th Edition):

Κουρνούτου, Γεωργία. “Μεταβολές στα επίπεδα των πολυαμινών του πεπτικού αδένα του μυδιού Mytilus galloprovincialis υπό την επίδραση οξειδωτικού στρες.” 2013. Masters Thesis, University of Patras. Accessed March 05, 2021. http://hdl.handle.net/10889/6237.

MLA Handbook (7^th Edition):

Κουρνούτου, Γεωργία. “Μεταβολές στα επίπεδα των πολυαμινών του πεπτικού αδένα του μυδιού Mytilus galloprovincialis υπό την επίδραση οξειδωτικού στρες.” 2013. Web. 05 Mar 2021.

Vancouver:

Κουρνούτου �. Μεταβολές στα επίπεδα των πολυαμινών του πεπτικού αδένα του μυδιού Mytilus galloprovincialis υπό την επίδραση οξειδωτικού στρες. [Internet] [Masters thesis]. University of Patras; 2013. [cited 2021 Mar 05]. Available from: http://hdl.handle.net/10889/6237.

Council of Science Editors:

Κουρνούτου �. Μεταβολές στα επίπεδα των πολυαμινών του πεπτικού αδένα του μυδιού Mytilus galloprovincialis υπό την επίδραση οξειδωτικού στρες. [Masters Thesis]. University of Patras; 2013. Available from: http://hdl.handle.net/10889/6237

11. Ramani, David. Citrulline et métabolisme des polyamines : quelle place pour la N-carbamoylputrescine? : Citrulline and polyamine metabolism : what role for N-carbamoylputrescine ?.

Degree: Docteur es, Nutrition, 2012, Université Paris Descartes – Paris V

URL: http://www.theses.fr/2012PA05P632

►

La citrulline est un acide aminé dont les propriétés anaboliques n’ont été mises en évidence que très récemment. Ces propriétés pourraient relever en particulier de… (more)

▼

La citrulline est un acide aminé dont les propriétés anaboliques n’ont été mises en évidence que très récemment. Ces propriétés pourraient relever en particulier de son rôle de précurseur direct de l’arginine. L’arginine est un acide aminé central du métabolisme des mammifères, notamment comme précurseur de nombreuses molécules exerçant des fonctions physiologiques essentielles, en particulier les polyamines, que ce soit les polyamines « classiques » putrescine, spermidine et spermine, dérivés de décarboxylation de l’ornithine impliquées entre autres dans la prolifération cellulaire, ou l’agmatine, dérivé de la décarboxylation de l’arginine. Toutefois, les interactions métaboliques entre citrulline et polyamines restent encore peu connues. Le but de ce travail a été d’explorer le lien métabolique entre citrulline et polyamines, selon deux démarches distinctes.La première a été d’évaluer l’influence d’une complémentation prolongée (3 mois) en citrulline sur le métabolisme tissulaire des polyamines « classiques » chez le rat âgé. La citrulline n’affecte que de manière modérée le métabolisme des polyamines, principalement en corrigeant les altérations liées au vieillissement.La seconde, par analogie avec la putrescine et l’agmatine, a exploré l’implication du dérivé décarboxylé de la citrulline, la N-carbamoyl-putrescine (NCP) dans le métabolisme des mammifères après avoir mis au point une technique de dosage de celle-ci dans les milieux biologiques. Nous n’avons pas pu mettre en évidence la présence de NCP dans des tissus de rats ayant reçu de la citrulline par voie orale ou par voie parentérale suggèrant soit une absence de synthèse soit un métabolisme extrêmement rapide de la NCP. Par ailleurs, la NCP administrée par voie orale à des doses de 5 et 50 mg/kg/j ne semble pas induire de toxicité ni d’effets biologiques majeurs mais la démonstration de son absorption digestive reste à faire. Cependant, la NCP exerce quelques effets modérés sur le métabolisme des acides aminés et des polyamines, l’évaluation de doses plus élevées ou l’administration par voie parentérale permettrait de confirmer ces observations.

The anabolic properties of the amino acid citrulline have only been recently demonstrated. These properties may result at least in part from its role as an arginine precursor. Arginine plays a central role in mammalian amino acid metabolism, notably as the precusor of many molecules involved in essential physiological functions, such as polyamines either “classical” ones, namely putrescine, spermidine, and spermine, derived from ornithine decarboxylation and notably involved in cell proliferation, or more recently-discovered molecules such as agmatine, the decarboxylation derivative of arginine. Metabolic interactions between citrulline and polyamines are still mostly unknown.The aim of this work was to explore the metabolic link between citrulline and polyamines using two different strategies.First, the influence of a long term citrulline supplementation on tissue “classical” polyamine metabolism in aged rats…

Advisors/Committee Members: Bandt, Jean-Pascal de (thesis director).

Subjects/Keywords: N-Carbamoylputrescine; Polyamines; Citrulline; Metabolisme; 572.465

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Ramani, D. (2012). Citrulline et métabolisme des polyamines : quelle place pour la N-carbamoylputrescine? : Citrulline and polyamine metabolism : what role for N-carbamoylputrescine ?. (Doctoral Dissertation). Université Paris Descartes – Paris V. Retrieved from http://www.theses.fr/2012PA05P632

Chicago Manual of Style (16^th Edition):

Ramani, David. “Citrulline et métabolisme des polyamines : quelle place pour la N-carbamoylputrescine? : Citrulline and polyamine metabolism : what role for N-carbamoylputrescine ?.” 2012. Doctoral Dissertation, Université Paris Descartes – Paris V. Accessed March 05, 2021. http://www.theses.fr/2012PA05P632.

MLA Handbook (7^th Edition):

Ramani, David. “Citrulline et métabolisme des polyamines : quelle place pour la N-carbamoylputrescine? : Citrulline and polyamine metabolism : what role for N-carbamoylputrescine ?.” 2012. Web. 05 Mar 2021.

Vancouver:

Ramani D. Citrulline et métabolisme des polyamines : quelle place pour la N-carbamoylputrescine? : Citrulline and polyamine metabolism : what role for N-carbamoylputrescine ?. [Internet] [Doctoral dissertation]. Université Paris Descartes – Paris V; 2012. [cited 2021 Mar 05]. Available from: http://www.theses.fr/2012PA05P632.

Council of Science Editors:

Ramani D. Citrulline et métabolisme des polyamines : quelle place pour la N-carbamoylputrescine? : Citrulline and polyamine metabolism : what role for N-carbamoylputrescine ?. [Doctoral Dissertation]. Université Paris Descartes – Paris V; 2012. Available from: http://www.theses.fr/2012PA05P632

Montana State University

12. Hulla, Janis Elaine. Polyamine mutagenesis : evidence for a unique mechanism.

Degree: MS, College of Letters & Science, 1981, Montana State University

URL: https://scholarworks.montana.edu/xmlui/handle/1/3652

Subjects/Keywords: Polyamines.; Chemical mutagenesis.

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Hulla, J. E. (1981). Polyamine mutagenesis : evidence for a unique mechanism. (Masters Thesis). Montana State University. Retrieved from https://scholarworks.montana.edu/xmlui/handle/1/3652

Chicago Manual of Style (16^th Edition):

Hulla, Janis Elaine. “Polyamine mutagenesis : evidence for a unique mechanism.” 1981. Masters Thesis, Montana State University. Accessed March 05, 2021. https://scholarworks.montana.edu/xmlui/handle/1/3652.

MLA Handbook (7^th Edition):

Hulla, Janis Elaine. “Polyamine mutagenesis : evidence for a unique mechanism.” 1981. Web. 05 Mar 2021.

Vancouver:

Hulla JE. Polyamine mutagenesis : evidence for a unique mechanism. [Internet] [Masters thesis]. Montana State University; 1981. [cited 2021 Mar 05]. Available from: https://scholarworks.montana.edu/xmlui/handle/1/3652.

Council of Science Editors:

Hulla JE. Polyamine mutagenesis : evidence for a unique mechanism. [Masters Thesis]. Montana State University; 1981. Available from: https://scholarworks.montana.edu/xmlui/handle/1/3652

13. O'BRIEN, KATIE. Sterol Regulatory Element Binding Proteins; Central Metabolic Regulators of Natural Killer Cells.

Degree: School of Biochemistry & Immunology. Discipline of Biochemistry, 2020, Trinity College Dublin

URL: http://hdl.handle.net/2262/91660

► Natural killer (NK) cells have important functions in the immune response against pathogen- infected and transformed cells. NK cell metabolism is crucial for NK cell… (more)

▼ Natural killer (NK) cells have important functions in the immune response against pathogen- infected and transformed cells. NK cell metabolism is crucial for NK cell effector functions. In this study sterol regulatory element binding protein (SREBP) transcription factors were identified as a central regulator of NK cell metabolism and function. Through genetic and pharmacological approaches, SREBP activity was shown to be essential for NK cell glycolysis and oxidative phosphorylation in response to cytokine-stimulation with IL2 and IL12. Furthermore, SREBP activity was identified as being essential for the functional responses of NK cells ? both in vitro and in vivo. It was previously identified that NK cells utilise a specific metabolic configuration known as the citrate-malate-shuttle (CMS) to support their metabolism and function. SREBP controls expression of two key components of the CMS - the enzyme ATP citrate lyase and the antiporter, SLC25A1. However, it was considered that SREBP may be controlling other aspects of NK cell metabolism. Indeed, it was identified that SREBP activity is required for optimal polyamine biosynthesis in IL2/IL12-stimulated NK cells. Further investigation involving the pharmacological inhibition of polyamine synthesis , identified that polyamines were required for NK cell metabolism and function and that hypusination - a polyamine-dependent post-translational modification, was crucial for the ability of NK cells to upregulate oxidative phosphorylation levels in response to stimulation. Exploration of the mechanism linking SREBP activity to polyamine synthesis revealed that SREBP activity is required for the upregulation of cMYC expression in response to IL2/IL12 in NK cells. cMYC is well documented to have a role in the control of the de novo polyamine synthesis pathway. Therefore, this work has identified a crucial role for SREBP transcription factors in the control of NK cell metabolic and functional responses. Advisors/Committee Members: Finlay, David.

Subjects/Keywords: SREBP; NK cell; Metabolism; Polyamines; Immunometabolism

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APA (6^th Edition):

O'BRIEN, K. (2020). Sterol Regulatory Element Binding Proteins; Central Metabolic Regulators of Natural Killer Cells. (Thesis). Trinity College Dublin. Retrieved from http://hdl.handle.net/2262/91660

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

O'BRIEN, KATIE. “Sterol Regulatory Element Binding Proteins; Central Metabolic Regulators of Natural Killer Cells.” 2020. Thesis, Trinity College Dublin. Accessed March 05, 2021. http://hdl.handle.net/2262/91660.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

O'BRIEN, KATIE. “Sterol Regulatory Element Binding Proteins; Central Metabolic Regulators of Natural Killer Cells.” 2020. Web. 05 Mar 2021.

Vancouver:

O'BRIEN K. Sterol Regulatory Element Binding Proteins; Central Metabolic Regulators of Natural Killer Cells. [Internet] [Thesis]. Trinity College Dublin; 2020. [cited 2021 Mar 05]. Available from: http://hdl.handle.net/2262/91660.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

O'BRIEN K. Sterol Regulatory Element Binding Proteins; Central Metabolic Regulators of Natural Killer Cells. [Thesis]. Trinity College Dublin; 2020. Available from: http://hdl.handle.net/2262/91660

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Hong Kong

14. Yao, Kwok-ming. Purification and characterization of ornithine decarbozylase fromtetrahymena thermophila.

Degree: 1986, University of Hong Kong

URL: http://hdl.handle.net/10722/32901

Subjects/Keywords: Polyamines.; Tetrahymena.; Decarboxylases.

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APA (6^th Edition):

Yao, K. (1986). Purification and characterization of ornithine decarbozylase fromtetrahymena thermophila. (Thesis). University of Hong Kong. Retrieved from http://hdl.handle.net/10722/32901

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Yao, Kwok-ming. “Purification and characterization of ornithine decarbozylase fromtetrahymena thermophila.” 1986. Thesis, University of Hong Kong. Accessed March 05, 2021. http://hdl.handle.net/10722/32901.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Yao, Kwok-ming. “Purification and characterization of ornithine decarbozylase fromtetrahymena thermophila.” 1986. Web. 05 Mar 2021.

Vancouver:

Yao K. Purification and characterization of ornithine decarbozylase fromtetrahymena thermophila. [Internet] [Thesis]. University of Hong Kong; 1986. [cited 2021 Mar 05]. Available from: http://hdl.handle.net/10722/32901.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Yao K. Purification and characterization of ornithine decarbozylase fromtetrahymena thermophila. [Thesis]. University of Hong Kong; 1986. Available from: http://hdl.handle.net/10722/32901

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Brock University

15. Fixon-Owoo, Solomon N. K. Synthesis of 4-hydroxycinnamic amides of di-, tri-, and tetraamines : potential insect toxins .

Degree: Department of Chemistry, 2000, Brock University

URL: http://hdl.handle.net/10464/1345

► The monoconjugates of phenolic acids (i.e. coumaric acid) with polyamines such as spermidine and spermine are strikingly similar to some toxins from spiders and predatory… (more)

Subjects/Keywords: Polyamines; Amides; Insecticides.

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APA (6^th Edition):

Fixon-Owoo, S. N. K. (2000). Synthesis of 4-hydroxycinnamic amides of di-, tri-, and tetraamines : potential insect toxins . (Thesis). Brock University. Retrieved from http://hdl.handle.net/10464/1345

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Fixon-Owoo, Solomon N K. “Synthesis of 4-hydroxycinnamic amides of di-, tri-, and tetraamines : potential insect toxins .” 2000. Thesis, Brock University. Accessed March 05, 2021. http://hdl.handle.net/10464/1345.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Fixon-Owoo, Solomon N K. “Synthesis of 4-hydroxycinnamic amides of di-, tri-, and tetraamines : potential insect toxins .” 2000. Web. 05 Mar 2021.

Vancouver:

Fixon-Owoo SNK. Synthesis of 4-hydroxycinnamic amides of di-, tri-, and tetraamines : potential insect toxins . [Internet] [Thesis]. Brock University; 2000. [cited 2021 Mar 05]. Available from: http://hdl.handle.net/10464/1345.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Fixon-Owoo SNK. Synthesis of 4-hydroxycinnamic amides of di-, tri-, and tetraamines : potential insect toxins . [Thesis]. Brock University; 2000. Available from: http://hdl.handle.net/10464/1345

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Georgia Tech

16. Hartong, Bradley H. Epi-halohydrin modified polyamines as mordant agents for alkaline rosin sizing.

Degree: PhD, Institute of Paper Science and Technology, 2004, Georgia Tech

URL: http://hdl.handle.net/1853/7032

► Many grades of paper have shifted from acid to alkaline production conditions. This change excludes the usage of traditional alum-rosin sizing chemistry. In some cases… (more)

▼ Many grades of paper have shifted from acid to alkaline production conditions. This change excludes the usage of traditional alum-rosin sizing chemistry. In some cases however, rosin sizing could prove beneficial if it could be used under neutral to alkaline paper making conditions. One effort toward this goal has been the use of epi-halohydrin modified polyamine/polyaminoamide in place of alum. The optimum sizing conditions, as well as the mechanism that modified polyamines/polyaminoamides use to size rosin, is investigated. Polyethyleneimine modified with epi-chlorohydrin was used in these studies. Rosin dosage was found to be the main variable that controlled the degree of sizing achieved. As the only hydrophobic material added, this was expected. In addition to rosin dosage, the polymer and pH had significant impacts on this sizing chemistry. Laboratory data shows that the synthesis procedure for and structure of polyethyleneimine-epi-cholohydrin (PEI-epi) impacts the degree of sizing achieved. Use of different modification techniques and degrees of modification illustrated several key factory regarding the polymer as a rosin sizing mordant. Prevention or removal of by-products from epi-chlorohydrin imporves the sizing efficiency of PEI-epi with rosin. A critical molecular weight of the PEI-epi was found to be important to sizing. Higher charge density on PEI-epi provides better the sizing efficiency. In addition to the polymer, other important factors where examined. The pH of the stock impacted the degree of sizing and permanency of the sizing achieved. Varying stock pH showed changes in sizing that are consistent with effects seen for rosin retention and of PAE as a wet strength agent. Low and high pH levels also caused reversion of the sizing effect. A pilot trial of the PEI-epi-rosin sizing chemistry was conducted at the Herty Foundation. The pilot trial shows that PEI-epi-rosin can reach the required level of sizing for linerboard. Trial results showed that calcium carbonate filled sheets could be sized by this chemistry. No significant impact on strength is seen from the sizing chemistry at the required dosage levels. As would be expected, addition of filler diminishes sheet strength. No sizing reversion was seen from any samples of this trial. Size reversion at low stock pH indicated that acid catalytic cleavage of an ester bond could have been occurring. Addition of acid or base to permanently sized sheets showed significant levels of reversion, similar to that seen for low stock pH sheets. This provides further supporting eviden ce for the formation and importance of an ester bond between rosin and PEI-epi. FTIR spectra were collected for model compounds to further examine the presence and importance of ester bonds. An ester bond was found to form at room temperature with aging or immediately with drying. Further, the ester bonds were found to decrease when the sample was subjected to acidic or basic… Advisors/Committee Members: Yulin Deng, Ph.D. Assoc. Professor - Georgia Tech (School, Arthur Ragauskas (Committee), Lucian Lucia, Sujit Banerjee, and Jeffery Hsieh.

Subjects/Keywords: Alkalinity; Sizing; Polyamines

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APA (6^th Edition):

Hartong, B. H. (2004). Epi-halohydrin modified polyamines as mordant agents for alkaline rosin sizing. (Doctoral Dissertation). Georgia Tech. Retrieved from http://hdl.handle.net/1853/7032

Chicago Manual of Style (16^th Edition):

Hartong, Bradley H. “Epi-halohydrin modified polyamines as mordant agents for alkaline rosin sizing.” 2004. Doctoral Dissertation, Georgia Tech. Accessed March 05, 2021. http://hdl.handle.net/1853/7032.

MLA Handbook (7^th Edition):

Hartong, Bradley H. “Epi-halohydrin modified polyamines as mordant agents for alkaline rosin sizing.” 2004. Web. 05 Mar 2021.

Vancouver:

Hartong BH. Epi-halohydrin modified polyamines as mordant agents for alkaline rosin sizing. [Internet] [Doctoral dissertation]. Georgia Tech; 2004. [cited 2021 Mar 05]. Available from: http://hdl.handle.net/1853/7032.

Council of Science Editors:

Hartong BH. Epi-halohydrin modified polyamines as mordant agents for alkaline rosin sizing. [Doctoral Dissertation]. Georgia Tech; 2004. Available from: http://hdl.handle.net/1853/7032

Université de Sherbrooke

17. Benrezzak, Ouhida. Rôle de l'ornithine décarboxylase et des polyamines dans la croissance stimulée du pancréas exocrine par la caeruléine.

Degree: 1984, Université de Sherbrooke

URL: http://hdl.handle.net/11143/14907

► Cette recherche fut inspirée par les résultats de plusieurs travaux qui ont démontré que les hormones gastrointestinales, sécrétine, cholécystokinine et son analogue la caeruléine favorisaient… (more)

▼ Cette recherche fut inspirée par les résultats de plusieurs travaux qui ont démontré que les hormones gastrointestinales, sécrétine, cholécystokinine et son analogue la caeruléine favorisaient la croissance du pancréas exocrine et entreprise dans le but d'élucider le mode d'action de la caeruléine qui demeure encore inconnu. En raison de la forte association des polyamines (putrescine, spermidine et spermine) à la croissance cellulaire, nous avons vérifié si la caeruléine n'agirait pas via la synthèse accrue de ces polycations pour stimuler la croissance du pancréas exocrine. Pour ce faire, des rats (150-155 g) ont reçu dans l'eau de boisson, 2 jours avant et durant le traitement à la caeruléine (1 µg/kg de poids corporel) et de l'x DFMO (2% solution), un inhibiteur spécifique et irréversible de l'enzyme qui initie la biosynthèse des polyamines: l'ornithine décarboxylase (ODC). Après sacrifice, les pancréas furent prélevés, pesés et évalués quant à leurs contenus totaux en polyamines. Alors que l'hyperplasie fut déterminée par les mesures du contenu total de l'organe en ADN et par la synthèse de l'ADN, l'hypertrophie fut établie par l'évaluation de la masse cellulaire, des concentrations en enzymes, en protéines et en ARN. Les principaux points qui ont été étudiés et analysés furent: L'effet de la caeruléine seule et combinée au DFMO sur les contenus en polyamines du pancréas. L'effet de la caeruléine seule et combinée au DFMO sur les paramètres d'hyperplasie et d'hypertrophie (deux phases de la croissance). L'effet de l'administration des polyamines exogènes, notamment la putrescine sur la croissance du pancréas. Les résultats obtenus ont montré principalement que: La caeruléine augmente significativement les contenus en putrescine (319%) après 12 heures et de 217% après 24 heures de traitement. Le DFMO (2%) réduit et abolit ces augmentations après 12 et 24 heures de traitement. La spermidine et la spermine sont augmentées respectivement par 63% et 50% après 96 heures, période de déclin de la putrescine. Tandis que la spermidine fut inhibée par le DFM0, la spermine demeura inchangée. Des injections sous-cutanées aux 8 heures à la caeruléine ont provoqué des augmentations significatives de tous les paramètres d'hypertrophie et d'hyperplasie après 48 et 96 heures. Le DFMO seul n'affecte aucun de ces paramètres. L'inhibiteur de l'ODC est cependant capable de réduire la croissance stimulée du pancréas ayant réduit significativement: le poids pancréatique total et les indices d'hyperplasie après 48 et 96 heures des changements majeurs ont été parallèlement observés au niveau des concentrations de la chymotrypsine après 48 et 96 heures. Une simple et unique injection intrapéritonéale de putrescine à raison de 300 µmoles/kg/jour a complètement aboli les diminutions de l'ADN total causées par l' x-DFMO. Étant donné que les augmentations de putrescine semblent suivre celles de l'ADN, que l'inhibition de l'ODC, l'abolition et la réduction des niveaux en putrescine et spermidine ont affecté l'hyperplasie et que… Advisors/Committee Members: Morisset, Jean (advisor).

Subjects/Keywords: Ornithine décarboxylase; Polyamines

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Benrezzak, O. (1984). Rôle de l'ornithine décarboxylase et des polyamines dans la croissance stimulée du pancréas exocrine par la caeruléine. (Masters Thesis). Université de Sherbrooke. Retrieved from http://hdl.handle.net/11143/14907

Chicago Manual of Style (16^th Edition):

Benrezzak, Ouhida. “Rôle de l'ornithine décarboxylase et des polyamines dans la croissance stimulée du pancréas exocrine par la caeruléine.” 1984. Masters Thesis, Université de Sherbrooke. Accessed March 05, 2021. http://hdl.handle.net/11143/14907.

MLA Handbook (7^th Edition):

Benrezzak, Ouhida. “Rôle de l'ornithine décarboxylase et des polyamines dans la croissance stimulée du pancréas exocrine par la caeruléine.” 1984. Web. 05 Mar 2021.

Vancouver:

Benrezzak O. Rôle de l'ornithine décarboxylase et des polyamines dans la croissance stimulée du pancréas exocrine par la caeruléine. [Internet] [Masters thesis]. Université de Sherbrooke; 1984. [cited 2021 Mar 05]. Available from: http://hdl.handle.net/11143/14907.

Council of Science Editors:

Benrezzak O. Rôle de l'ornithine décarboxylase et des polyamines dans la croissance stimulée du pancréas exocrine par la caeruléine. [Masters Thesis]. Université de Sherbrooke; 1984. Available from: http://hdl.handle.net/11143/14907

University of Aberdeen

18. Acheampong, Paul. Polyamines : effect of diet on blood concentrations and evaluation of their role as biomarkers of stroke.

Degree: Thesis (M.D.), 2010, University of Aberdeen

URL: https://abdn.alma.exlibrisgroup.com/view/delivery/44ABE_INST/12153029000005941 ; https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.531842

► The main aims were to: optimise the method for extracting polyamine from food and blood products; study the effect of diet (after oral loading with… (more)

Subjects/Keywords: 612.3; Polyamines; Stroke

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APA (6^th Edition):

Acheampong, P. (2010). Polyamines : effect of diet on blood concentrations and evaluation of their role as biomarkers of stroke. (Doctoral Dissertation). University of Aberdeen. Retrieved from https://abdn.alma.exlibrisgroup.com/view/delivery/44ABE_INST/12153029000005941 ; https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.531842

Chicago Manual of Style (16^th Edition):

Acheampong, Paul. “Polyamines : effect of diet on blood concentrations and evaluation of their role as biomarkers of stroke.” 2010. Doctoral Dissertation, University of Aberdeen. Accessed March 05, 2021. https://abdn.alma.exlibrisgroup.com/view/delivery/44ABE_INST/12153029000005941 ; https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.531842.

MLA Handbook (7^th Edition):

Acheampong, Paul. “Polyamines : effect of diet on blood concentrations and evaluation of their role as biomarkers of stroke.” 2010. Web. 05 Mar 2021.

Vancouver:

Acheampong P. Polyamines : effect of diet on blood concentrations and evaluation of their role as biomarkers of stroke. [Internet] [Doctoral dissertation]. University of Aberdeen; 2010. [cited 2021 Mar 05]. Available from: https://abdn.alma.exlibrisgroup.com/view/delivery/44ABE_INST/12153029000005941 ; https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.531842.

Council of Science Editors:

Acheampong P. Polyamines : effect of diet on blood concentrations and evaluation of their role as biomarkers of stroke. [Doctoral Dissertation]. University of Aberdeen; 2010. Available from: https://abdn.alma.exlibrisgroup.com/view/delivery/44ABE_INST/12153029000005941 ; https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.531842

19. Hatmi, Saloua. Impact du déficit hydrique sur les réponses de défense et la sensibilité de la vigne à Botrytis cinerea : rôle de la dégradation des polyamines. : Impact of water deficiency on defense responses and susceptibility of grapevine to Botrytis cinerea : Role of polyamine oxidation.

Degree: Docteur es, Sciences - STS, 2013, Reims; Université de Tunis. Faculté des sciences de Tunis

URL: http://www.theses.fr/2013REIMS035

►

La vigne est sujette à de nombreuses contraintes biotiques et abiotiques face auxquelles elle devra optimiser ses stratégies de défense, en favorisant parfois des interconnections… (more)

▼

La vigne est sujette à de nombreuses contraintes biotiques et abiotiques face auxquelles elle devra optimiser ses stratégies de défense, en favorisant parfois des interconnections entre les réponses adaptatives au stress abiotique et la gestion de la réponse immune face à un pathogène. Dans cette étude nous avons évalué l'effet du stress hydrique (par privation d'eau) sur différentes réactions adaptatives au stress, mais aussi sur des réponses de défense et sur la sensibilité des feuilles et des baies de la vigne à Botrytis cinerea. Ces réactions ont été suivies en utilisant des boutures végétatives de deux cépages : cv. Meski (MSK), tolérant à la sécheresse et cv. Chardonnay (CHR), sensible. La relation entre les réponses au stress hydrique et la réponse immune a également été recherchée au moyen de feuilles de vitroplants et de baies isolées de Chardonnay exposées à des osmotica : le polyéthylène glycol (PEG) et une forte concentration en saccahrose (SUC). Les résultats montrent que le stress hydrique/osmotique conduit à des modifications physiologiques et biochimiques importantes dans les feuilles et les baies mûres de vigne. L'amélioration de la tolérance chez MSK est associée à une faible inhibition de l'activité photosynthétique, une altération du profil des acides aminés et un catabolisme actif des polyamines (PAs) comparé au CHR. Ces résultats suggèrent un rôle potentiel des déviations métaboliques observées dans les processus de tolérance de la vigne au stress osmotique. La tolérance du MSK au déficit hydrique est également corrélée à une forte induction des réponses de défense accumulation de resvératrol et e-viniférine, expression de certains gènes de défense dont STS, Gluc (PR-2), Chit-4c (PR-3) et PR-5 dans les feuilles, ainsi qu'à une faible sensibilité à B. cinerea.Ces résultats suggèrent un lien étroit entre la tolérance au déficit hydrique et la capacité de la vigne à exprimer davantage ses mécanismes de défense et à résister mieux à B. cinerea. Des expériences pharmacologiques ont montré qu'en situation de stress hydrique/osmotique, le catabolisme des PAs, via des diamine- et PA-oxydases, est impliqué dans la régulation de l'homéostasie des PAs et de l'expression des réactions de défense. L'application du stress osmotique avant l'infection des feuilles par B. cinerea potentialise l'accumulation des PAs en réduisant fortement leur dégradation. Ces effets sont corrélés à une réduction de l'amplitude des réponses de défense après infection, et à une sensibilité accrue à B. cinerea. Ces résultats rendent compte (1) de l'importance des stress abiotiques dans la régulation de la réponse immune chez la vigne et sa résistance à B. cinerea et (2) du fait que le niveau des réponses de défense osmo-induites et la résistance au pathogène sont tributaires, au moins en partie, de certains mécanismes adaptatifs au stress, comme c'est le cas ici des voies de dégradation des polyamines.

Grapevine is often exposed to both biotic and abiotic stresses, and it will optimize defense strategies by favoing sometimes the…

Advisors/Committee Members: Aziz, Aziz (thesis director).

Subjects/Keywords: Vigne; Stress hydrique; Botrytis cinerea; Polyamines; Réponse immune; Grapevine; Water stress; Botrytis cinerea; Polyamines; Immune responses; 634.8

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Hatmi, S. (2013). Impact du déficit hydrique sur les réponses de défense et la sensibilité de la vigne à Botrytis cinerea : rôle de la dégradation des polyamines. : Impact of water deficiency on defense responses and susceptibility of grapevine to Botrytis cinerea : Role of polyamine oxidation. (Doctoral Dissertation). Reims; Université de Tunis. Faculté des sciences de Tunis. Retrieved from http://www.theses.fr/2013REIMS035

Chicago Manual of Style (16^th Edition):

Hatmi, Saloua. “Impact du déficit hydrique sur les réponses de défense et la sensibilité de la vigne à Botrytis cinerea : rôle de la dégradation des polyamines. : Impact of water deficiency on defense responses and susceptibility of grapevine to Botrytis cinerea : Role of polyamine oxidation.” 2013. Doctoral Dissertation, Reims; Université de Tunis. Faculté des sciences de Tunis. Accessed March 05, 2021. http://www.theses.fr/2013REIMS035.

MLA Handbook (7^th Edition):

Hatmi, Saloua. “Impact du déficit hydrique sur les réponses de défense et la sensibilité de la vigne à Botrytis cinerea : rôle de la dégradation des polyamines. : Impact of water deficiency on defense responses and susceptibility of grapevine to Botrytis cinerea : Role of polyamine oxidation.” 2013. Web. 05 Mar 2021.

Vancouver:

Hatmi S. Impact du déficit hydrique sur les réponses de défense et la sensibilité de la vigne à Botrytis cinerea : rôle de la dégradation des polyamines. : Impact of water deficiency on defense responses and susceptibility of grapevine to Botrytis cinerea : Role of polyamine oxidation. [Internet] [Doctoral dissertation]. Reims; Université de Tunis. Faculté des sciences de Tunis; 2013. [cited 2021 Mar 05]. Available from: http://www.theses.fr/2013REIMS035.

Council of Science Editors:

Hatmi S. Impact du déficit hydrique sur les réponses de défense et la sensibilité de la vigne à Botrytis cinerea : rôle de la dégradation des polyamines. : Impact of water deficiency on defense responses and susceptibility of grapevine to Botrytis cinerea : Role of polyamine oxidation. [Doctoral Dissertation]. Reims; Université de Tunis. Faculté des sciences de Tunis; 2013. Available from: http://www.theses.fr/2013REIMS035

20. Carriel Lopez, Diego. Etude sur la relation fonction-structure de la lysine décarboxylase de Pseudomonas aeruginosa : Structure-function relationships of the lysine decarboxylase from Pseudomonas aeruginosa.

Degree: Docteur es, Biologie Structurale et Nanobiologie, 2017, Université Grenoble Alpes (ComUE)

URL: http://www.theses.fr/2017GREAV011

► La lysine décarboxylase (LDC) appartient à une famille d'enzymes décamériques dépendantes du cofacteur PLP qui sont connus pour catalyser la réaction transformant la L-Lysine en… (more)

▼ La lysine décarboxylase (LDC) appartient à une famille d'enzymes décamériques dépendantes du cofacteur PLP qui sont connus pour catalyser la réaction transformant la L-Lysine en cadavérine tout en consommant un proton. Dans les entérobactéries comme Escherichia coli, nous trouvons deux paralogues, LdcI et LdcC. LdcI permet de faire face à la bactérie au conditions hostile de pH acide lors du passage à travers du tract gastro-intestinale. LdcC est produite pendant la phase stationnaire et aussi quand les bactéries font face aux traitements antibiotiques. La cadavérine produite par les LDCs est connue pour protéger les bactéries du stress oxydant. Cela s’explique par le fait que la cadavérine bloque les porines de la membrane externe, réduisant ainsi la perméabilité des molécules responsables du stress acides et oxydant. L'activité des LDCs chez E. coli est coordonnée avec la réponse stringente qui est mise en place lorsque les microorganismes sont dans des conditions pauvres en nutriments, afin d’éviter l’épuisement intracellulaire de la L-Lysine nécessaire pour la synthèse des protéines. Cependant, cette inhibition peut être levée par la formation d'un complexe en forme de cage avec son partenaire RavA, permettant ainsi aux bactéries de faire face aux stress multiples. Etant donné que la réponse au stress est importante pour que les bactéries puissent exhiber leur pathogénicité, nous nous sommes demandés si la bactérie opportuniste Pseudomonas aeruginosa pourrait employer LdcA pour contrer des conditions de stress qui ont déjà été décrites pour LdcI chez les entérobactéries. Au cours de ma thèse, nous avons abordé cette question en utilisant différentes approches complémentaires. Tout d'abord, nous avons utilisé des fusions promoteur-gène et de l'analyse par Western-blot pour déterminer les conditions dans lesquelles le gène ldcA a été exprimé et sa protéine synthétisée. Nous avons pu observer que ldcA est exprimé sur la phase stationnaire de croissance dans des conditions aérobies en milieux riches et également pendant des conditions anaérobies de respiration avec nitrate. Nous avons également confirmé que l'expression de ldcA est régulée par ArgR et elle est induite complètement lorsque l’acide aminé L-arginine est présente dans le milieu de croissance. Même si nous avons trouvé que les conditions de stress n'induisent pas l'expression de ldcA, nous avons obtenu de nouvelles données suggérant que d'autres mécanismes de régulation tels que le système de quorum sensing dépendant des quinolones (PQS) pourraient être impliqués dans l'expression de ldcA. En utilisant des souches mutantes de ldcA et son complémentée, nous avons évalué si LdcA était impliqué dans la réponse au stress acide et oxydatif. Bien que les données obtenues à l'aide des expériences dans notre laboratoire et des technologies à haut débit (Biolog) aient révélé que LdcA ne présente pas les mêmes fonctions que LdcI, nous avons découvert que la cadavérine produite par LdcA est nécessaire pour la croissance en milieu minimal avec L- Glutamate comme… Advisors/Committee Members: Gutsche, Irina (thesis director), Elsen, Sylvie (thesis director).

Subjects/Keywords: Physiologie bactérienne; Lysine Decarboxylase; Pseudomonas; E.coli; Polyamines; Bacterial Physiology; Lysine Decarboxylase; Pseudomonas; E. coli; Polyamines; 570

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Carriel Lopez, D. (2017). Etude sur la relation fonction-structure de la lysine décarboxylase de Pseudomonas aeruginosa : Structure-function relationships of the lysine decarboxylase from Pseudomonas aeruginosa. (Doctoral Dissertation). Université Grenoble Alpes (ComUE). Retrieved from http://www.theses.fr/2017GREAV011

Chicago Manual of Style (16^th Edition):

Carriel Lopez, Diego. “Etude sur la relation fonction-structure de la lysine décarboxylase de Pseudomonas aeruginosa : Structure-function relationships of the lysine decarboxylase from Pseudomonas aeruginosa.” 2017. Doctoral Dissertation, Université Grenoble Alpes (ComUE). Accessed March 05, 2021. http://www.theses.fr/2017GREAV011.

MLA Handbook (7^th Edition):

Carriel Lopez, Diego. “Etude sur la relation fonction-structure de la lysine décarboxylase de Pseudomonas aeruginosa : Structure-function relationships of the lysine decarboxylase from Pseudomonas aeruginosa.” 2017. Web. 05 Mar 2021.

Vancouver:

Carriel Lopez D. Etude sur la relation fonction-structure de la lysine décarboxylase de Pseudomonas aeruginosa : Structure-function relationships of the lysine decarboxylase from Pseudomonas aeruginosa. [Internet] [Doctoral dissertation]. Université Grenoble Alpes (ComUE); 2017. [cited 2021 Mar 05]. Available from: http://www.theses.fr/2017GREAV011.

Council of Science Editors:

Carriel Lopez D. Etude sur la relation fonction-structure de la lysine décarboxylase de Pseudomonas aeruginosa : Structure-function relationships of the lysine decarboxylase from Pseudomonas aeruginosa. [Doctoral Dissertation]. Université Grenoble Alpes (ComUE); 2017. Available from: http://www.theses.fr/2017GREAV011

University of Florida

21. Geddes, Ryan D. Improving Fermentation of Pretreated Biomass by Reducing Inhibitor Concentrations and Increasing Microbial Biocatalyst Tolerance.

Degree: PhD, Microbiology and Cell Science, 2014, University of Florida

URL: https://ufdc.ufl.edu/UFE0047363

► Lignocellulosic biomass provides an abundant source of carbohydrates that can be utilized for the production of fuels and chemicals. However, plant biomass is resistant to… (more)

Subjects/Keywords: Biomass; Enzymes; Ethanol; Ethanol production; Fermentation; Plasmids; Polyamines; Pretreatment; Sugars; Toxicity; biomass – detoxify – ethanol – furfural – hydrolysate – inhibitors – lignocellulose – polyamines

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Geddes, R. D. (2014). Improving Fermentation of Pretreated Biomass by Reducing Inhibitor Concentrations and Increasing Microbial Biocatalyst Tolerance. (Doctoral Dissertation). University of Florida. Retrieved from https://ufdc.ufl.edu/UFE0047363

Chicago Manual of Style (16^th Edition):

Geddes, Ryan D. “Improving Fermentation of Pretreated Biomass by Reducing Inhibitor Concentrations and Increasing Microbial Biocatalyst Tolerance.” 2014. Doctoral Dissertation, University of Florida. Accessed March 05, 2021. https://ufdc.ufl.edu/UFE0047363.

MLA Handbook (7^th Edition):

Geddes, Ryan D. “Improving Fermentation of Pretreated Biomass by Reducing Inhibitor Concentrations and Increasing Microbial Biocatalyst Tolerance.” 2014. Web. 05 Mar 2021.

Vancouver:

Geddes RD. Improving Fermentation of Pretreated Biomass by Reducing Inhibitor Concentrations and Increasing Microbial Biocatalyst Tolerance. [Internet] [Doctoral dissertation]. University of Florida; 2014. [cited 2021 Mar 05]. Available from: https://ufdc.ufl.edu/UFE0047363.

Council of Science Editors:

Geddes RD. Improving Fermentation of Pretreated Biomass by Reducing Inhibitor Concentrations and Increasing Microbial Biocatalyst Tolerance. [Doctoral Dissertation]. University of Florida; 2014. Available from: https://ufdc.ufl.edu/UFE0047363

Virginia Tech

22. Szumanski, Maria B. W. Characterization of structure, function and regulation of the speB gene in Escherichia coli.

Degree: PhD, Biochemistry and Nutrition, 1989, Virginia Tech

URL: http://hdl.handle.net/10919/54438

► The speB gene of E. coli encodes agmatine ureohydrolase (AUH). AUH catalyses the hydrolysis of agmatine to urea and putrescine in a polyamine biosynthetic pathway.… (more)

▼ The speB gene of E. coli encodes agmatine ureohydrolase (AUH). AUH catalyses the hydrolysis of agmatine to urea and putrescine in a polyamine biosynthetic pathway. The plasmid pKA5, derived from an E. coli genomic library, was the source of a 2.97 kb restriction fragment containing the speB gene. Sequencing of this fragment revealed three intact open reading frames, ORF1 and ORF2 on one strand and ORF3 on the opposite strand, as well as a truncated open reading frame, ORF4, which terminated 92 kb upstream from ORF3. ORF2 and ORF3 were convergent, and overlapped by 85% of their sequence. ORF1 and ORF3 were separated by a sequence of two imperfect repeats containing four palindromes, three of which were overlapping. ORF3 represented the coding sequence of the speB gene. Two transcripts were detected from the speB gene: a shorter transcript, initiated 101 bp upstream from ORF3, and a polycistronic message, coding for ORF3 and ORF4. The short transcript was abundantly expressed when ORF4 sequences were deleted, but when ORF4 and its upstream sequences were present, the polycistronic message predominated and the amount of the monocistronic message was drastically reduced. The promoter producing the shorter transcript required only a -12 TATACT sequence for activity. Deletion of a 460 bp fragment comprising the 5'-region of ORF1 from a plasmid containing ORF1, ORF2 and speB reduced the activity of AUH by 83%. This fragment contained two divergently oriented promoters. The presence of ORF1 did not stimulate ß-galactosidase encoded by the speB promoter fused to lacΖ. Agmatine induced transcription from speB but not from the ORF4 nor the ORF1 promoters. cAMP caused an 88% reduction in the AUH activity of wild type E. coli K-12 but had no effect on the activity of plasmid encoded AUH. The activity of neither the speB nor the ORF4 promoters fused to lacΖ or phoA were influenced by cAMP; in contrast, the lacZ promoter fused to lacZ or phoA was stimulated by cAMP. Thus, the role of cAMP and CRP on speB expression is indirect and limited to a single copy state.

Subjects/Keywords: LD5655.V856 1989.S987; Polyamines; Polyamines in the body

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Szumanski, M. B. W. (1989). Characterization of structure, function and regulation of the speB gene in Escherichia coli. (Doctoral Dissertation). Virginia Tech. Retrieved from http://hdl.handle.net/10919/54438

Chicago Manual of Style (16^th Edition):

Szumanski, Maria B W. “Characterization of structure, function and regulation of the speB gene in Escherichia coli.” 1989. Doctoral Dissertation, Virginia Tech. Accessed March 05, 2021. http://hdl.handle.net/10919/54438.

MLA Handbook (7^th Edition):

Szumanski, Maria B W. “Characterization of structure, function and regulation of the speB gene in Escherichia coli.” 1989. Web. 05 Mar 2021.

Vancouver:

Szumanski MBW. Characterization of structure, function and regulation of the speB gene in Escherichia coli. [Internet] [Doctoral dissertation]. Virginia Tech; 1989. [cited 2021 Mar 05]. Available from: http://hdl.handle.net/10919/54438.

Council of Science Editors:

Szumanski MBW. Characterization of structure, function and regulation of the speB gene in Escherichia coli. [Doctoral Dissertation]. Virginia Tech; 1989. Available from: http://hdl.handle.net/10919/54438

University of Pretoria

23. [No author]. Gene expression profiling of polyamine-depleted Plasmodium falciparum .

Degree: 2008, University of Pretoria

URL: http://upetd.up.ac.za/thesis/available/etd-12132007-103643/

► Polyamines play an important role in DNA, RNA and protein synthesis as well as a variety of other biological processes (cell division, differentiation and death)… (more)

▼ Polyamines play an important role in DNA, RNA and protein synthesis as well as a variety of other biological processes (cell division, differentiation and death) as outlined in Chapter 1. Assaraf and co-workers (1984) demonstrated that treatment with DFMO resulted in the inhibition of polyamine biosynthesis as well as schizogony arrest in P. falciparum. However, they did not elaborate on any other consequences that polyamine depletion could exert on the parasite. This dissertation aims to elucidate the significance of the inhibition of polyamine biosynthesis within P. falciparum by using differential transcriptome profiling. Suppression subtractive hybridisation generated transcripts which were potentially up-and down-regulated due to endogenous polyamine depletion within the human malaria parasite P. falciparum. The resulting transcripts were subjected to a restriction enzyme analysis and those with unique digestion profiles were selected and sequenced. The sequences were analysed using PlasmoDB to identify the genomic sequences to which they were best matched. To confirm that the selected transcripts were indeed differentially expressed a reverse virtual Northern dot blot was performed. Transcripts for proteins involved in protein processing, methionine and polyamine metabolism, various transporters, proteins involved in cellular differentiation and signal transduction were found to be upregulated in the absences of polyamines. This could be suggestive of a metabolic response induced by the parasite in order to overcome this deficiency. Polyamines seem to influence protein synthesis and haemoglobin degradation as well since depletion of endogenous polyamines within the parasite seems to result in increased food vacuole acidification, haemoglobin degradation, transport of proteins to the cytoplasm and protein synthesis and stabilisation. The majority of downregulated transcripts were found to be involved in cell-cell adhesion and erythrocyte invasion, protein processing and transport indicating that these processes are dependent on polyamines. Further validation of these findings by microarray as well as proteomic analysis will need to be undertaken. These results validate that polyamines do play an essential role in the cellular biology of the parasite. They also confirm that the inhibition of polyamine biosynthesis is a viable route to undertake in the search for new and improved antimalarial targets. This would be especially useful if it was combined with other antimalarials and their synergistic effects were investigated by transcriptomic, proteomic and bioinformatic analysis Advisors/Committee Members: Prof A I Louw (advisor), Dr L Birkholtz (advisor).

Subjects/Keywords: Suppression subtractive hybridisation; Malaria; Transcriptome analysis; Polyamines; Differential expression; UCTD

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APA (6^th Edition):

author], [. (2008). Gene expression profiling of polyamine-depleted Plasmodium falciparum . (Masters Thesis). University of Pretoria. Retrieved from http://upetd.up.ac.za/thesis/available/etd-12132007-103643/

Chicago Manual of Style (16^th Edition):

author], [No. “Gene expression profiling of polyamine-depleted Plasmodium falciparum .” 2008. Masters Thesis, University of Pretoria. Accessed March 05, 2021. http://upetd.up.ac.za/thesis/available/etd-12132007-103643/.

MLA Handbook (7^th Edition):

author], [No. “Gene expression profiling of polyamine-depleted Plasmodium falciparum .” 2008. Web. 05 Mar 2021.

Vancouver:

author] [. Gene expression profiling of polyamine-depleted Plasmodium falciparum . [Internet] [Masters thesis]. University of Pretoria; 2008. [cited 2021 Mar 05]. Available from: http://upetd.up.ac.za/thesis/available/etd-12132007-103643/.

Council of Science Editors:

author] [. Gene expression profiling of polyamine-depleted Plasmodium falciparum . [Masters Thesis]. University of Pretoria; 2008. Available from: http://upetd.up.ac.za/thesis/available/etd-12132007-103643/

University of Pretoria

24. Niemand, Jandeli. Biochemical characterisation of putrescine and spermidine uptake as a potential therapeutic target against the human malaria parasite, Plasmodium falciparum .

Degree: 2012, University of Pretoria

URL: http://upetd.up.ac.za/thesis/available/etd-05252012-112525/

► Plasmodium falciparum causes the most severe form of human malaria, and the continual development of resistance of this parasite to current anti-malarial drugs underpins a… (more)

▼ Plasmodium falciparum causes the most severe form of human malaria, and the continual development of resistance of this parasite to current anti-malarial drugs underpins a pressing need for the discovery of novel chemotherapeutic approaches. Polyamines and their biosynthetic enzymes are present at high levels in rapidly proliferating cells, including cancer cells and protozoan parasites. Inhibition of the malaria parasite’s polyamine biosynthesis pathway causes cytostatic arrest in the trophozoite stage, but does not cure infections in vivo. This may be due to the salvage of exogenous polyamines from the host, replenishing the intracellular polyamine pool; however the mechanism(s) of polyamine uptake by the intraerythrocytic parasite are not well understood. In this study the uptake of the polyamines putrescine and spermidine into P. falciparum-infected erythrocytes (iRBC) well as into P. falciparum parasites functionally isolated from their host cell by saponin-permeabilisation of the erythrocyte membrane was investigated using radioisotope flux techniques. While the characteristics of transport of putrescine into infected erythrocytes were similar to those of transport into uninfected erythrocytes, spermidine entered iRBC in part via the ‘new permeation pathways’ induced by the parasite in the erythrocyte membrane. Both putrescine and spermidine were taken up across the plasma membrane of isolated parasites via a saturable, temperature-dependent process that showed competition between different polyamines as well as the polyamine precursor ornithine and basic amino acids. Inhibition of polyamine biosynthesis led to increased total uptake of both putrescine and spermidine. The influx of putrescine and spermidine into isolated parasites was independent of Na+ but increased with increasing pH and showed a marked dependence on the membrane potential, decreasing with membrane depolarisation and increasing with membrane hyperpolarisation. Both anthracene and polyamine derivatives have been shown to have anti-malarial activity. Anthracene-polyamine conjugates have been developed with the aim of utilising the polyamine uptake mechanisms of cancer cells to deliver the cytotoxic anthracene moieties to these cells. Here, several anthracene-polyamine conjugates showed promising anti-malarial activity. These compounds inhibited parasite proliferation with IC50 values in the nM range, and caused an arrest in the cell cycle, as well as a decrease in the mitochondrial membrane potential. Cytotoxicity could not be reversed by the addition of exogenous polyamines, nor did the conjugates have an effect on intracellular polyamine levels. This doctoral study showed that P. falciparum parasites not only synthesise polyamines, but can also acquire putrescine and spermidine from the extracellular environment and paves the way for interfering with polyamine metabolism as an anti-parasitic strategy. Advisors/Committee Members: Birkholtz, Lyn-Marie (advisor), Louw, Abraham Izak (advisor).

Subjects/Keywords: Human malaria parasite; Plasmodium falciparum; Biosynthetic enzymes; Polyamines; UCTD

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APA (6^th Edition):

Niemand, J. (2012). Biochemical characterisation of putrescine and spermidine uptake as a potential therapeutic target against the human malaria parasite, Plasmodium falciparum . (Doctoral Dissertation). University of Pretoria. Retrieved from http://upetd.up.ac.za/thesis/available/etd-05252012-112525/

Chicago Manual of Style (16^th Edition):

Niemand, Jandeli. “Biochemical characterisation of putrescine and spermidine uptake as a potential therapeutic target against the human malaria parasite, Plasmodium falciparum .” 2012. Doctoral Dissertation, University of Pretoria. Accessed March 05, 2021. http://upetd.up.ac.za/thesis/available/etd-05252012-112525/.

MLA Handbook (7^th Edition):

Niemand, Jandeli. “Biochemical characterisation of putrescine and spermidine uptake as a potential therapeutic target against the human malaria parasite, Plasmodium falciparum .” 2012. Web. 05 Mar 2021.

Vancouver:

Niemand J. Biochemical characterisation of putrescine and spermidine uptake as a potential therapeutic target against the human malaria parasite, Plasmodium falciparum . [Internet] [Doctoral dissertation]. University of Pretoria; 2012. [cited 2021 Mar 05]. Available from: http://upetd.up.ac.za/thesis/available/etd-05252012-112525/.

Council of Science Editors:

Niemand J. Biochemical characterisation of putrescine and spermidine uptake as a potential therapeutic target against the human malaria parasite, Plasmodium falciparum . [Doctoral Dissertation]. University of Pretoria; 2012. Available from: http://upetd.up.ac.za/thesis/available/etd-05252012-112525/

University of California – San Diego

25. Van, Jenny Kim. Part 1: Method Development for the Analysis of Polyamines by Derivatives with Benzoyl Chloride by HPLC Tandem Mass Spectrometry Part 2: Detection of Volatile Organic Compound Tracers in Human Breath.

Degree: Chemistry (Joint Doctoral SDSU), 2015, University of California – San Diego

URL: http://www.escholarship.org/uc/item/5gx5p1r8

► Part 1: Development and validation of an analytical method by liquid chromatography-tandem mass spectrometry to detect putrescine, cadaverine, spermidine, spermine, n8-acetylspermidine, and n1-acetylspermine as benzyl… (more)

▼ Part 1: Development and validation of an analytical method by liquid chromatography-tandem mass spectrometry to detect putrescine, cadaverine, spermidine, spermine, n8-acetylspermidine, and n1-acetylspermine as benzyl amide derivatives in biological samples were completed. The derivatization conditions were optimized for all PAs, and method validation was completed for putrescine (PUT) and spermidine (SPD) using isotope dilution techniques. The method exhibited: high accuracy (<±15% error) and precision (<±10% RSD); product stability of >22 days; linearity within concentration range of 0.40-50 ng/mL for PUT and 0.42-530 ng/mL for SPD; level of detection (LOD) of 2.8 and 2.4 picograms (pg); and level of quantitation (LOQ) of 20 and 21 pg for PUT and SPD, respectively. Matrix effects and interferences were shown to not affect accuracy. The method developed provided superior with shorter analysis time than other published methods. Levels of PUT and SPD were determined to be 5.75 ± 0.1 and 43.8 ± 1 ng/mL, respectively in a sample as small as 4 fruit flies. Part 2: This project was a proof of concept study that used breath component analysis (BCA) to determine if a unique volatile organic compound (VOC) could be measured in human breath 72 hours or longer following a brief exposure. A portable breathing mask with a pressurized canister was constructed to provide 1-2 ppm exposures of the tracer chemicals to the subjects. An EBC sample collection device and canister cleaning system were modified to provide clean sample blanks. Analytical protocols were established to collect BCA from subjects using canisters and adsorbent traps. The chemicals tested and the maximum duration they were detectable in a subjects’ breath (days) were methoxyflurane (1.2), enflurane (7.7), sevoflurane (1.2), α,α,α-trifluorotoluene (14), 4-chlorobenzotrifluoride (7.2), and 3-fluorobenzotrifluoride (1.2). The rate constants for the most promising candidate chemicals followed a one-compartment (first order kinetics) model. Other compounds of similar chemical properties are potential candidates for further study. The applications of this proof of concept study may lead to the development of tools for use in toxicology, forensics, and security.

Subjects/Keywords: Chemistry; Breath Component Analysis; GC; HPLC; Mass Spectrometry; Polyamines; Tracers

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Van, J. K. (2015). Part 1: Method Development for the Analysis of Polyamines by Derivatives with Benzoyl Chloride by HPLC Tandem Mass Spectrometry Part 2: Detection of Volatile Organic Compound Tracers in Human Breath. (Thesis). University of California – San Diego. Retrieved from http://www.escholarship.org/uc/item/5gx5p1r8

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Van, Jenny Kim. “Part 1: Method Development for the Analysis of Polyamines by Derivatives with Benzoyl Chloride by HPLC Tandem Mass Spectrometry Part 2: Detection of Volatile Organic Compound Tracers in Human Breath.” 2015. Thesis, University of California – San Diego. Accessed March 05, 2021. http://www.escholarship.org/uc/item/5gx5p1r8.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Van, Jenny Kim. “Part 1: Method Development for the Analysis of Polyamines by Derivatives with Benzoyl Chloride by HPLC Tandem Mass Spectrometry Part 2: Detection of Volatile Organic Compound Tracers in Human Breath.” 2015. Web. 05 Mar 2021.

Vancouver:

Van JK. Part 1: Method Development for the Analysis of Polyamines by Derivatives with Benzoyl Chloride by HPLC Tandem Mass Spectrometry Part 2: Detection of Volatile Organic Compound Tracers in Human Breath. [Internet] [Thesis]. University of California – San Diego; 2015. [cited 2021 Mar 05]. Available from: http://www.escholarship.org/uc/item/5gx5p1r8.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Van JK. Part 1: Method Development for the Analysis of Polyamines by Derivatives with Benzoyl Chloride by HPLC Tandem Mass Spectrometry Part 2: Detection of Volatile Organic Compound Tracers in Human Breath. [Thesis]. University of California – San Diego; 2015. Available from: http://www.escholarship.org/uc/item/5gx5p1r8

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

26. Sampaio, Valdomiro de Freitas. Efeitos da desnutrição protéica precoce e da estimulação ambiental sobre parâmetros bioquímicos e comportamentais em ratos.

Degree: PhD, Psicobiologia, 2009, University of São Paulo

URL: http://www.teses.usp.br/teses/disponiveis/59/59134/tde-27082009-120622/ ;

►

O presente estudo investigou os efeitos da desnutrição protéica e estimulação ambiental sobre parâmetros bioquímicos em ratos. Foram quantificadas as concentrações de poliaminas no córtex… (more)

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O presente estudo investigou os efeitos da desnutrição protéica e estimulação ambiental sobre parâmetros bioquímicos em ratos. Foram quantificadas as concentrações de poliaminas no córtex frontal, estriado e hipocampo, corticosterona plasmática e atividade da sintase do óxido nítrico no hipocampo. O comportamento exploratório foi analisado no labirinto em cruz elevado (LCE). Os animais receberam dietas contendo 16% (controles - C) ou 6% (desnutridos - D) de proteína e foram divididos em grupos estimulados ou não estimulados. A desnutrição elevou o nível de corticosterona circulante, diminuiu o conteúdo protéico plasmático e diminuiu a ansiedade. Os ratos não estimulados testados no LCE apresentaram nível elevado de corticosterona circulante, diminuída atividade da NOS no hipocampo, menor conteúdo protéico e de poliaminas no córtex frontal, estriado e hipocampo. A estimulação diminuiu o número de entradas nos braços abertos no LCE nos animais do grupo D e aumentou o número de entradas nos braços fechados nos animais do grupo C. A estimulação aumentou a frequência e tempo gasto em comportamentos de avaliação de risco. Estes resultados sugerem que ambos, a desnutrição e o teste no LCE são situações estressantes, como indicado pela elevação dos níveis de corticosterona. Estes resultados são consistentes com menor ansiedade e/ou maior impulsividade nos animais do grupo D.

The present study investigated the effects of protein malnutrition and environmental stimulation on the biochemical and behavioral parameters in rats. It was measured the polyamines concentration in frontal cortex, striatum and hippocampus, as well as the plasma corticosterone levels. The exploratory behavior was analyzed using the elevated plus-maze (EPM) test. Rats received either 16% (well-nourished - W) or 6% (malnourished - M) protein diets and were divided into stimulated or non-stimulated groups. Malnutrition increased corticosterone levels, decreased plasma protein content and anxiety. Non-stimulated rats tested in the EPM increased corticosterone levels, decreased hippocampal NOS activity, decreased frontal cortex, striatum and hippocampus protein and polyamines contents. Stimulation decreased open arm entries in the EPM in M animals and increased the closed arm entries in the W ones. Stimulation increased frequency and time spent in risk-assessment behaviors. These results suggest that both malnutrition and EPM testing are stressing situations as indicated by increased corticosterone levels. These results are consistent with lower anxiety and/or higher impulsiveness in M animals.

Advisors/Committee Members: Almeida, Sebastião de Sousa.

Subjects/Keywords: Ansiedade; Anxiety; Corticosterona; Corticosterone; Desnutrição; Malnutrition; Poliaminas; Polyamines

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Sampaio, V. d. F. (2009). Efeitos da desnutrição protéica precoce e da estimulação ambiental sobre parâmetros bioquímicos e comportamentais em ratos. (Doctoral Dissertation). University of São Paulo. Retrieved from http://www.teses.usp.br/teses/disponiveis/59/59134/tde-27082009-120622/ ;

Chicago Manual of Style (16^th Edition):

Sampaio, Valdomiro de Freitas. “Efeitos da desnutrição protéica precoce e da estimulação ambiental sobre parâmetros bioquímicos e comportamentais em ratos.” 2009. Doctoral Dissertation, University of São Paulo. Accessed March 05, 2021. http://www.teses.usp.br/teses/disponiveis/59/59134/tde-27082009-120622/ ;.

MLA Handbook (7^th Edition):

Sampaio, Valdomiro de Freitas. “Efeitos da desnutrição protéica precoce e da estimulação ambiental sobre parâmetros bioquímicos e comportamentais em ratos.” 2009. Web. 05 Mar 2021.

Vancouver:

Sampaio VdF. Efeitos da desnutrição protéica precoce e da estimulação ambiental sobre parâmetros bioquímicos e comportamentais em ratos. [Internet] [Doctoral dissertation]. University of São Paulo; 2009. [cited 2021 Mar 05]. Available from: http://www.teses.usp.br/teses/disponiveis/59/59134/tde-27082009-120622/ ;.

Council of Science Editors:

Sampaio VdF. Efeitos da desnutrição protéica precoce e da estimulação ambiental sobre parâmetros bioquímicos e comportamentais em ratos. [Doctoral Dissertation]. University of São Paulo; 2009. Available from: http://www.teses.usp.br/teses/disponiveis/59/59134/tde-27082009-120622/ ;

Temple University

27. Moncada Benavides, Camilo Andres. EFFECT OF NICOTINE ON LUNG S-ADENOSYLMETHIONINE AND PNEUMOCYSTIS PNEUMONIA DEVELOPMENT.

Degree: PhD, 2012, Temple University

URL: http://digital.library.temple.edu/u?/p245801coll10,206623

►

Biochemistry

Infection with "Pneumocystis" causes a ≥ 99% depletion of plasma S-adenosylmethionine (AdoMet) levels in both "Pneumocystis" pneumonia (PcP) animal models and patients. AdoMet is… (more)

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Biochemistry

Infection with "Pneumocystis" causes a ≥ 99% depletion of plasma S-adenosylmethionine (AdoMet) levels in both "Pneumocystis" pneumonia (PcP) animal models and patients. AdoMet is a critical cellular metabolic intermediate, with a pivotal role as methyl donor in a myriad of biochemical processes and necessary for the synthesis of the essential polyamines spermidine and spermine. In the target tissue of "Pneumocystis", the lung, levels of AdoMet were previously shown to be depleted experimentally using nicotine. Here we show that chronic administration of nicotine in an animal model of PcP resulted in decreased lung AdoMet content. Since "Pneumocystis" is dependent on this metabolite, PcP burden was also relived. We hypothesized that the underlying mechanism behind nicotine-induced AdoMet depletion was an increased consumption of AdoMet through the polyamine pathway where the increased activity of N-1-spermidine/spermine acetyl transferase raises the catabolic / anabolic cycling of polyamines, a process that utilizes AdoMet. In a critical test of our hypothesis, we found that blockage of polyamine metabolism via inhibition of the polyamine biosynthetic enzyme ornithine decarboxylase (ODC) hinders the effect of nicotine on lung AdoMet levels. Further support is provided by metabolite analyses showing nicotine to cause a strong diversion of AdoMet toward polyamine synthesis and away from methylation reactions; these shifts are also reversed by inhibition of ODC. Because the nicotine effect on "Pneumocystis" is so striking, we considered the possibility of tissue specificity. Using laser capture microdissection (LCM), we collected samples of lung alveolar regions (site of infection) and respiratory epithelium for controls. We found nicotine to cause increased ODC activity in alveolar regions but not airway epithelium; we conclude that tissue specificity likely contributes to the effect of nicotine on "Pneumocystis" pneumonia. Our studies demonstrate the feasibility of pharmacological manipulation of the polyamine pathway in order to reduce AdoMet levels in the lung and prompted the assessment of compounds alternative to nicotine with the potential to achieve a comparable effect. In vitro evaluation of the polyamine analog DENSPM along with putrescine in type II alveolar cell lines, indicates that although such a combination has the potential to induce polyamine flux, an apparent competition for the same polyamine transport system impairs simultaneous uptake of both compounds at effective concentrations. In conclusion, we showed that chronic nicotine administration causes reduction of AdoMet levels in rat lung following 21 days of treatment, by a mechanism involving the induction of polyamine flux, which is responsible of increased AdoMet utilization for polyamine biosynthesis. According to LCM-based analysis, this effect seems to be confined to the alveolar regions of the lung.

Temple University – Theses

Advisors/Committee Members: Merali, Salim, Chong, Parkson Lee-Gau, Soprano, Dianne R., Collins, Jimmy H., Yarlett, Nigel.

Subjects/Keywords: Biochemistry; Laser Capture Microdissection; Lung; Nicotine; Pneumocystis; Polyamines; S-Adenosylmethionine

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APA (6^th Edition):

Moncada Benavides, C. A. (2012). EFFECT OF NICOTINE ON LUNG S-ADENOSYLMETHIONINE AND PNEUMOCYSTIS PNEUMONIA DEVELOPMENT. (Doctoral Dissertation). Temple University. Retrieved from http://digital.library.temple.edu/u?/p245801coll10,206623

Chicago Manual of Style (16^th Edition):

Moncada Benavides, Camilo Andres. “EFFECT OF NICOTINE ON LUNG S-ADENOSYLMETHIONINE AND PNEUMOCYSTIS PNEUMONIA DEVELOPMENT.” 2012. Doctoral Dissertation, Temple University. Accessed March 05, 2021. http://digital.library.temple.edu/u?/p245801coll10,206623.

MLA Handbook (7^th Edition):

Moncada Benavides, Camilo Andres. “EFFECT OF NICOTINE ON LUNG S-ADENOSYLMETHIONINE AND PNEUMOCYSTIS PNEUMONIA DEVELOPMENT.” 2012. Web. 05 Mar 2021.

Vancouver:

Moncada Benavides CA. EFFECT OF NICOTINE ON LUNG S-ADENOSYLMETHIONINE AND PNEUMOCYSTIS PNEUMONIA DEVELOPMENT. [Internet] [Doctoral dissertation]. Temple University; 2012. [cited 2021 Mar 05]. Available from: http://digital.library.temple.edu/u?/p245801coll10,206623.

Council of Science Editors:

Moncada Benavides CA. EFFECT OF NICOTINE ON LUNG S-ADENOSYLMETHIONINE AND PNEUMOCYSTIS PNEUMONIA DEVELOPMENT. [Doctoral Dissertation]. Temple University; 2012. Available from: http://digital.library.temple.edu/u?/p245801coll10,206623

University of Alberta

28. Laplante, Carmen M. Site-selective N-methylation of resin bound terminal amines and borane reduction/iodine work-up: amino acid side chain compatibility and solution studies.

Degree: MS, Department of Chemistry, 2001, University of Alberta

URL: https://era.library.ualberta.ca/files/xs55mf16d

Subjects/Keywords: Oxidation-reduction reaction.; Polyamines.; Methylation.

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Laplante, C. M. (2001). Site-selective N-methylation of resin bound terminal amines and borane reduction/iodine work-up: amino acid side chain compatibility and solution studies. (Masters Thesis). University of Alberta. Retrieved from https://era.library.ualberta.ca/files/xs55mf16d

Chicago Manual of Style (16^th Edition):

Laplante, Carmen M. “Site-selective N-methylation of resin bound terminal amines and borane reduction/iodine work-up: amino acid side chain compatibility and solution studies.” 2001. Masters Thesis, University of Alberta. Accessed March 05, 2021. https://era.library.ualberta.ca/files/xs55mf16d.

MLA Handbook (7^th Edition):

Laplante, Carmen M. “Site-selective N-methylation of resin bound terminal amines and borane reduction/iodine work-up: amino acid side chain compatibility and solution studies.” 2001. Web. 05 Mar 2021.

Vancouver:

Laplante CM. Site-selective N-methylation of resin bound terminal amines and borane reduction/iodine work-up: amino acid side chain compatibility and solution studies. [Internet] [Masters thesis]. University of Alberta; 2001. [cited 2021 Mar 05]. Available from: https://era.library.ualberta.ca/files/xs55mf16d.

Council of Science Editors:

Laplante CM. Site-selective N-methylation of resin bound terminal amines and borane reduction/iodine work-up: amino acid side chain compatibility and solution studies. [Masters Thesis]. University of Alberta; 2001. Available from: https://era.library.ualberta.ca/files/xs55mf16d

University of Pretoria

29. Niemand, Jandeli. Biochemical characterisation of putrescine and spermidine uptake as a potential therapeutic target against the human malaria parasite, Plasmodium falciparum.

Degree: Biochemistry, 2012, University of Pretoria

URL: http://hdl.handle.net/2263/24986

► Plasmodium falciparum causes the most severe form of human malaria, and the continual development of resistance of this parasite to current anti-malarial drugs underpins a… (more)

▼ Plasmodium falciparum causes the most severe form of human malaria, and the continual development of resistance of this parasite to current anti-malarial drugs underpins a pressing need for the discovery of novel chemotherapeutic approaches. Polyamines and their biosynthetic enzymes are present at high levels in rapidly proliferating cells, including cancer cells and protozoan parasites. Inhibition of the malaria parasite’s polyamine biosynthesis pathway causes cytostatic arrest in the trophozoite stage, but does not cure infections in vivo. This may be due to the salvage of exogenous polyamines from the host, replenishing the intracellular polyamine pool; however the mechanism(s) of polyamine uptake by the intraerythrocytic parasite are not well understood. In this study the uptake of the polyamines putrescine and spermidine into P. falciparum-infected erythrocytes (iRBC) well as into P. falciparum parasites functionally isolated from their host cell by saponin-permeabilisation of the erythrocyte membrane was investigated using radioisotope flux techniques. While the characteristics of transport of putrescine into infected erythrocytes were similar to those of transport into uninfected erythrocytes, spermidine entered iRBC in part via the ‘new permeation pathways’ induced by the parasite in the erythrocyte membrane. Both putrescine and spermidine were taken up across the plasma membrane of isolated parasites via a saturable, temperature-dependent process that showed competition between different polyamines as well as the polyamine precursor ornithine and basic amino acids. Inhibition of polyamine biosynthesis led to increased total uptake of both putrescine and spermidine. The influx of putrescine and spermidine into isolated parasites was independent of Na+ but increased with increasing pH and showed a marked dependence on the membrane potential, decreasing with membrane depolarisation and increasing with membrane hyperpolarisation. Both anthracene and polyamine derivatives have been shown to have anti-malarial activity. Anthracene-polyamine conjugates have been developed with the aim of utilising the polyamine uptake mechanisms of cancer cells to deliver the cytotoxic anthracene moieties to these cells. Here, several anthracene-polyamine conjugates showed promising anti-malarial activity. These compounds inhibited parasite proliferation with IC50 values in the nM range, and caused an arrest in the cell cycle, as well as a decrease in the mitochondrial membrane potential. Cytotoxicity could not be reversed by the addition of exogenous polyamines, nor did the conjugates have an effect on intracellular polyamine levels. This doctoral study showed that P. falciparum parasites not only synthesise polyamines, but can also acquire putrescine and spermidine from the extracellular environment and paves the way for interfering with polyamine metabolism as an anti-parasitic strategy. Advisors/Committee Members: Birkholtz, Lyn-Marie (advisor), Louw, Abraham Izak (advisor).

Subjects/Keywords: Human malaria parasite; Plasmodium falciparum; Biosynthetic enzymes; Polyamines; UCTD

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Niemand, J. (2012). Biochemical characterisation of putrescine and spermidine uptake as a potential therapeutic target against the human malaria parasite, Plasmodium falciparum. (Doctoral Dissertation). University of Pretoria. Retrieved from http://hdl.handle.net/2263/24986

Chicago Manual of Style (16^th Edition):

Niemand, Jandeli. “Biochemical characterisation of putrescine and spermidine uptake as a potential therapeutic target against the human malaria parasite, Plasmodium falciparum.” 2012. Doctoral Dissertation, University of Pretoria. Accessed March 05, 2021. http://hdl.handle.net/2263/24986.

MLA Handbook (7^th Edition):

Niemand, Jandeli. “Biochemical characterisation of putrescine and spermidine uptake as a potential therapeutic target against the human malaria parasite, Plasmodium falciparum.” 2012. Web. 05 Mar 2021.

Vancouver:

Niemand J. Biochemical characterisation of putrescine and spermidine uptake as a potential therapeutic target against the human malaria parasite, Plasmodium falciparum. [Internet] [Doctoral dissertation]. University of Pretoria; 2012. [cited 2021 Mar 05]. Available from: http://hdl.handle.net/2263/24986.

Council of Science Editors:

Niemand J. Biochemical characterisation of putrescine and spermidine uptake as a potential therapeutic target against the human malaria parasite, Plasmodium falciparum. [Doctoral Dissertation]. University of Pretoria; 2012. Available from: http://hdl.handle.net/2263/24986

University of Oulu

30. Haghighi Poodeh, S. (Saeid). Novel pathomechanisms of intrauterine growth restriction in fetal alcohol syndrome in a mouse model.

Degree: 2016, University of Oulu

URL: http://urn.fi/urn:isbn:9789526213163

►

Abstract Fetal alcohol syndrome (FAS) is a pattern of anomalies in affected children due to maternal alcohol administration at vulnerable stages of fetal development. Intrauterine… (more)

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Abstract Fetal alcohol syndrome (FAS) is a pattern of anomalies in affected children due to maternal alcohol administration at vulnerable stages of fetal development. Intrauterine growth restriction and facial malformation are the presenting phenotypes of FAS. In this investigation, novel pathomechanisms of intrauterine growth restriction and facial malformation were the primary aims. We found by a FAS mouse model that AceCS1 gene expression and polyamines are the immediate targets of fetal alcohol exposure. The AceCS1 product is a precursor for lipid synthesis and protein acetylation and possibly, for polycation acetylation. We cloned the Mus musculus nuclear-cytosolic AceCS1 gene, and showed that its expression is developmentally regulated with a dynamic localization in the cytosolic and nuclear compartment. The enzyme plays an essential role in de novo synthesis of acetyl Coenzyme A. Fetal alcohol administration targets nutrient supplying networks, which are localized at critical barriers. The main findings were reduced surface of the labyrinthine zone, destruction of gap junctions in the hemotrichorial placenta, reduced syncytiotrophoblastic cell layers and loosening of interaction between cell layers and embryo endothelial cells, reduced Reichert’s membrane thickness with discontinued Reichert’s trophoblast and loss of interaction by Reichert’s-parietal cells, reduction of capillary network and reduced vascularization in the brain area, and perturbed neural crest migration and formation of neural tube defect. Alteration of angiogenesis -regulating proteins such as VEGF, PlGF, PECAM was detected in FAS, with no significant changes in placental angiogenesis of the labyrinthine zone, but up-regulation of VEGF/PlGF caused permeability changes in the placenta and yolk sac. On the other hand, the PECAM pool in embryos’ brain was reduced, which in turn led to decreased angiogenesis and vascularization.

Tiivistelmä Sikiön alkoholisyndrooma (engl. Fetal alcohol syndrome, FAS) on joukko muutoksia, joita esiintyy äidin raskaudenaikaisen alkoholin käytön seurauksena, kun käyttö osuu sikiökehityksen kannalta kriittiseen vaiheeseen. Kohdunsisäisen kasvun rajoittuminen ja kasvojen epämuodostumat ovat FAS:n tyypillisimpiä ilmentymiä. Tässä tutkimuksessa pyrittiin löytämään uusia patomekanismeja kohdunsisäisen kasvun rajoittumiselle ja kasvojen epämuodostumille. Hiiren FAS-mallin avulla selvisi, että sikiön altistuminen alkoholille vaikuttaa suoraan AceCS1-geenin ilmentymiseen ja polyamiinien pitoisuuteen. AceCS1-geenin tuote on esiaste lipidien synteesissä ja proteiinien asetylaatiossa sekä mahdollisesti myös polykationien asetylaatiossa. Työssä myös kloonattiin hiiren (Mus musculus) AceCS1-geeni, jonka tuotetta esiintyy sekä tumassa että solulimassa. Lisäksi osoitettiin, että geenin ekspressio oli kehityksen aikana säädelty tuottamaan entsyymiä dynaamisesti eri paikkoihin solussa. Entsyymillä on lisäksi merkittävä osuus asetyyli-koentsyymi-A:n de novo–synteesissä. Sikiön altistuminen alkoholille kohdistuu sellaisten…

Advisors/Committee Members: Savolainen, M. (Markku), Vainio, S. (Seppo), Salonurmi, T. (Tuire).

Subjects/Keywords: DFMO; Polyamines; Syncytiotrophoblast cells; polyamiinit; synsytiotrofoblastisolut; AceCS1; PECAM; PlGF; VEGF

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Haghighi Poodeh, S. (. (2016). Novel pathomechanisms of intrauterine growth restriction in fetal alcohol syndrome in a mouse model. (Doctoral Dissertation). University of Oulu. Retrieved from http://urn.fi/urn:isbn:9789526213163

Chicago Manual of Style (16^th Edition):

Haghighi Poodeh, S (Saeid). “Novel pathomechanisms of intrauterine growth restriction in fetal alcohol syndrome in a mouse model.” 2016. Doctoral Dissertation, University of Oulu. Accessed March 05, 2021. http://urn.fi/urn:isbn:9789526213163.

MLA Handbook (7^th Edition):

Haghighi Poodeh, S (Saeid). “Novel pathomechanisms of intrauterine growth restriction in fetal alcohol syndrome in a mouse model.” 2016. Web. 05 Mar 2021.

Vancouver:

Haghighi Poodeh S(. Novel pathomechanisms of intrauterine growth restriction in fetal alcohol syndrome in a mouse model. [Internet] [Doctoral dissertation]. University of Oulu; 2016. [cited 2021 Mar 05]. Available from: http://urn.fi/urn:isbn:9789526213163.

Council of Science Editors:

Haghighi Poodeh S(. Novel pathomechanisms of intrauterine growth restriction in fetal alcohol syndrome in a mouse model. [Doctoral Dissertation]. University of Oulu; 2016. Available from: http://urn.fi/urn:isbn:9789526213163

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Open Access Theses and Dissertations