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You searched for subject:(pollen staining). Showing records 1 – 3 of 3 total matches.

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1. Zu, Pengjuan. Effects of Nectar Production and Pollinator Assemblies on Mating Patterns in Orchids.

Degree: Biology Education Centre, 2011, Uppsala UniversityUppsala University

Pollinator visitation patterns should affect pollination success and mating patterns in flowering species. In the orchid family, about one third of the species do not provide any reward for their pollinators. Pollination by deceit is typically associated with low fruit set but may increase the chance of cross-pollination since the pollinator should soon leave the individual plant when there is no reward in the flowers. This may be beneficial if self-fertilisation results in inbreeding depression. I studied the mating patterns of one rewarding and one deceptive orchid in two closely related genera by tracking the fate of stained pollinia. I also conducted controlled crosses to estimate inbreeding depression. The results show that the deceptive orchid Dactylorhiza lapponica has lower pollination success, but higher cross-pollination rate (ca. 90%) than the nectariferous orchid Gymnadenia conopsea (ca. 18% cross-pollination). The results further suggest that in G. conopsea, nocturnal visitors mediate higher geitonogamous pollination rate (ca. 100%) than diurnal visitors (ca. 60%). In both study species, fruits produced from cross-pollination were heavier than fruits produced from selfing. Inbreeding depression for fruit mass did not differ significantly between the two species (δ = 0.21 in D. lapponica and δ = 0.29 in G. conopsea). These data support the hypothesis that pollination by deceit can enhance cross-pollination. A literature study including several rewarding and non-rewarding orchid species indicated lower geitonogamy in the deceptive orchids, but the difference was not statistically significant. 

Subjects/Keywords: Inbreeding depression; mating system; nectar; nectarless; orchid; pollen staining; pollinator assembly

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6th Edition):

Zu, P. (2011). Effects of Nectar Production and Pollinator Assemblies on Mating Patterns in Orchids. (Thesis). Uppsala UniversityUppsala University. Retrieved from http://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-159358

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16th Edition):

Zu, Pengjuan. “Effects of Nectar Production and Pollinator Assemblies on Mating Patterns in Orchids.” 2011. Thesis, Uppsala UniversityUppsala University. Accessed September 30, 2020. http://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-159358.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7th Edition):

Zu, Pengjuan. “Effects of Nectar Production and Pollinator Assemblies on Mating Patterns in Orchids.” 2011. Web. 30 Sep 2020.

Vancouver:

Zu P. Effects of Nectar Production and Pollinator Assemblies on Mating Patterns in Orchids. [Internet] [Thesis]. Uppsala UniversityUppsala University; 2011. [cited 2020 Sep 30]. Available from: http://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-159358.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Zu P. Effects of Nectar Production and Pollinator Assemblies on Mating Patterns in Orchids. [Thesis]. Uppsala UniversityUppsala University; 2011. Available from: http://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-159358

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation


North Carolina State University

2. Olsen, Richard Thomas. Utilizing Polyploidy for Developing Improved Nursery Crops: Restoring Fertility in Wide Hybrids, Limiting Fertility of Invasive Species, Embryo Culture of Triploids, Pest Resistance, and Inheritance of Ornamental Traits.

Degree: PhD, Horticultural Science, 2007, North Carolina State University

Multiple projects were conducted to investigate the potential for developing a breeding program utilizing species of Catalpa Scop. and Chilopsis D. Don. The efficacy of oryzalin was evaluated for inducing polyploidy and restoring fertility in the sterile, intergeneric hybrid xChitalpa tashkentensis Elias & Wisura [Catalpa bignonioides Walt. x Chilopsis linearis (Cav.) Sweet] 'Pink Dawn'. Submerging meristems in 150 μM oryzalin for up to 24 hours was effective at inducing tetraploids and cytochimeras. Pollen from the diploid cultivar was non-viable, but pollen from the polyploid stained and germinated as well as pollen from progenitor taxa. Polyploid xChitalpa were self-compatible yielding tetraploids when self pollinated and triploids when crossed with C. bignonioides, but reciprocal crosses with Chilopsis taxa failed. To increase recovery of triploids, we investigated germination of ovules and embryos at various harvest dates on Schenk and Hildebrandt (SH) basal salts supplemented with various medium components. Germination of triploid [(polyploid xChitalpa) x C. bignonioides] and tetraploid (selfed polyploid xChitalpa) embryos was greatest at 7 weeks after pollination on SH with 20 g•L⁺¹ sucrose and ≥ 1 μM gibberellic acid (GA₃). Triploids [Chilopsis linearis x (polyploid xChitalpa)] germinated < 5%. Additional studies were conducted to screen diverse taxa for resistance to powdery mildew (PM), Erysiphe elevata (Burr.) U. Braun & S. Takam, and catalpa sphinx larvae (CSL), Ceratomia catalpae (Boisduval). Twenty-four taxa from Catalpa (section Catalpa Paclt and Macrocatalpa Grisebach), Chilopsis, and xChitalpa were screened in 2004-05 for susceptibility to PM. Disease incidence and severity were recorded to calculate area under the disease progress curves (AUDPC) for each year. North American Catalpa spp. in sect. Catalpa, Chilopsis, and xChitalpa taxa were all moderate to highly susceptible to PM. Chinese Catalpa spp. in sect. Catalpa and West Indian sect. Macrocatalpa were resistant to PM. Hybrids among North American and Chinese Catalpa spp. in sect. Catalpa varied in susceptibility, indicating inheritance of partial resistance to PM. A no-choice feeding study conducted with CSL in 2005 found no differences in survival or growth of larvae reared on taxa from both sections of Catalpa, Chilopsis, and xChitalpa. Future breeding of xChitalpa can utilize the identified sources of resistance for PM; however, a source of resistance to CSL was not found. Triploids are generally infertile and may be deployed by breeders to limit invasive potential of introduced ornamentals. However, inheritance of ornamental traits can be complex at higher ploidy levels. Inheritance of two mutant foliage types, variegated and purple, was investigated for diploid, triploid and tetraploid tutsan (Hypericum androsaemum L.). Fertility of progeny was evaluated with pollen viability tests, percent fruit set, and germinative capacity of seed from specific crosses. Segregation… Advisors/Committee Members: G. Craig Yencho, Committee Member (advisor), Dennis J. Werner, Committee Member (advisor), Thomas G. Ranney, Committee Chair (advisor), David A. Danehower, Committee Member (advisor).

Subjects/Keywords: autopolyploidy; flow cytometry; Tecomeae; Bignoniaceae; Guttiferae; pollen germination; pollen staining; allopolyploidy

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6th Edition):

Olsen, R. T. (2007). Utilizing Polyploidy for Developing Improved Nursery Crops: Restoring Fertility in Wide Hybrids, Limiting Fertility of Invasive Species, Embryo Culture of Triploids, Pest Resistance, and Inheritance of Ornamental Traits. (Doctoral Dissertation). North Carolina State University. Retrieved from http://www.lib.ncsu.edu/resolver/1840.16/3495

Chicago Manual of Style (16th Edition):

Olsen, Richard Thomas. “Utilizing Polyploidy for Developing Improved Nursery Crops: Restoring Fertility in Wide Hybrids, Limiting Fertility of Invasive Species, Embryo Culture of Triploids, Pest Resistance, and Inheritance of Ornamental Traits.” 2007. Doctoral Dissertation, North Carolina State University. Accessed September 30, 2020. http://www.lib.ncsu.edu/resolver/1840.16/3495.

MLA Handbook (7th Edition):

Olsen, Richard Thomas. “Utilizing Polyploidy for Developing Improved Nursery Crops: Restoring Fertility in Wide Hybrids, Limiting Fertility of Invasive Species, Embryo Culture of Triploids, Pest Resistance, and Inheritance of Ornamental Traits.” 2007. Web. 30 Sep 2020.

Vancouver:

Olsen RT. Utilizing Polyploidy for Developing Improved Nursery Crops: Restoring Fertility in Wide Hybrids, Limiting Fertility of Invasive Species, Embryo Culture of Triploids, Pest Resistance, and Inheritance of Ornamental Traits. [Internet] [Doctoral dissertation]. North Carolina State University; 2007. [cited 2020 Sep 30]. Available from: http://www.lib.ncsu.edu/resolver/1840.16/3495.

Council of Science Editors:

Olsen RT. Utilizing Polyploidy for Developing Improved Nursery Crops: Restoring Fertility in Wide Hybrids, Limiting Fertility of Invasive Species, Embryo Culture of Triploids, Pest Resistance, and Inheritance of Ornamental Traits. [Doctoral Dissertation]. North Carolina State University; 2007. Available from: http://www.lib.ncsu.edu/resolver/1840.16/3495


North Carolina State University

3. Jones, Jeffrey Robert. Investigating Prevalence, Induction, and Fertility of Polyploid Rhododendron L. and the Development of Protocols for Vegetative Propagation.

Degree: MS, Horticultural Science, 2008, North Carolina State University

Studies were conducted to determine the ploidy levels of specific Rhododendron taxa, to develop a simple and effective ex-vitro method for inducing polyploidy in Rhododendron seedlings, and to evaluate the effect of increased ploidy level on pollen fertility. A diverse collection of species, hybrids, and cultivars in the Hymenanthes (elepidote rhododendrons), Rhododendron (lepidote rhododendrons), Pentanthera (deciduous azaleas), and Tsutsusi (evergreen azaleas) subgenera were surveyed to determine ploidy level and relative genome size using flow cytometry. In instances where ploidy levels were inconsistent with past literature, chromosome counts were made on young root tips to substantiate findings. Mean 2C holoploid genome sizes varied as a function of subgenus and ploidy level. Relative genome sizes (2C) within ploidy level, for a given subgenus, had a narrow range providing clear distinction among ploidy levels. Mean 1Cx monoploid genome size was conserved across ploidy levels within a subgenus. Polyploidy was found to be common in the genus Rhododendron and considerably more prevalent in the subgenus Pentanthera than previously known. Particularly noteworthy were the findings that R. occidentale includes both diploid and tetraploid individuals and that R. atlanticum and R. austrinum are predominantly tetraploid species. Induction studies were then completed with the goal of obtaining artificial tetraploids from diploid x diploid hybridizations. The effectiveness of using repeated treatments of an oryzalin suspension in a warm agar solution applied directly to apical shoots of Rhododendron seedlings to induce polyploidy was tested. Apical meristems of hybrid seedlings were subjected to 1, 2, 3, or 4 applications of oryzalin separated by 4 day intervals or left untreated (control). The results of this study demonstrated that the method of applying a suspension of oryzalin in warm, semi-solid agar to the shoots of Rhododendron seedlings was an effective method for inducing polyploidy. Although single applications resulted in some polyploid plants, multiple applications increased efficacy for some of the taxa studied. Treatments resulted in a range of ploidy levels, from 2x to 8x, including mixaploids (cytochimeras). The effect of increased ploidy level on pollen fertility and the occurrence of unreduced gametes in triploid taxa were also studied. Pollen viability was compared between corresponding progenitor and polyploid taxa by staining pollen with 1% acetocarmine (w⁄v) for 15 minutes. The existence of unreduced gametes in triploid taxa was determined by the presence of dyad and/or monad pollen grains. The results demonstrated that the fertility of polyploid Rhododendron can be highly variable and that the induction of polyploidy may either enhance or compromise fertility. Moreover, some triploids produced viable, unreduced pollen (as high as 5%), allowing for the possible utilization of these plants in breeding programs. Documentation of polyploid taxa, improved methods for inducing… Advisors/Committee Members: Thomas G, Ranney, Committee Chair (advisor), Anthony V. LeBude, Committee Member (advisor), Dennis J. Werner, Committee Member (advisor).

Subjects/Keywords: cutting propagation; polyploid induction; unreduced gametes; pollen staining; flow cytometry; rhododendron; allopolyploidy; autopolyploidy

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6th Edition):

Jones, J. R. (2008). Investigating Prevalence, Induction, and Fertility of Polyploid Rhododendron L. and the Development of Protocols for Vegetative Propagation. (Thesis). North Carolina State University. Retrieved from http://www.lib.ncsu.edu/resolver/1840.16/828

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16th Edition):

Jones, Jeffrey Robert. “Investigating Prevalence, Induction, and Fertility of Polyploid Rhododendron L. and the Development of Protocols for Vegetative Propagation.” 2008. Thesis, North Carolina State University. Accessed September 30, 2020. http://www.lib.ncsu.edu/resolver/1840.16/828.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7th Edition):

Jones, Jeffrey Robert. “Investigating Prevalence, Induction, and Fertility of Polyploid Rhododendron L. and the Development of Protocols for Vegetative Propagation.” 2008. Web. 30 Sep 2020.

Vancouver:

Jones JR. Investigating Prevalence, Induction, and Fertility of Polyploid Rhododendron L. and the Development of Protocols for Vegetative Propagation. [Internet] [Thesis]. North Carolina State University; 2008. [cited 2020 Sep 30]. Available from: http://www.lib.ncsu.edu/resolver/1840.16/828.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Jones JR. Investigating Prevalence, Induction, and Fertility of Polyploid Rhododendron L. and the Development of Protocols for Vegetative Propagation. [Thesis]. North Carolina State University; 2008. Available from: http://www.lib.ncsu.edu/resolver/1840.16/828

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

.