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You searched for subject:(phytoplasma pyri). One record found.

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Technical University of Lisbon

1. Marques, Ana Isabel Ambrósio. Pesquisa de fitoplasmas das pomóideas e prunóideas em Portugal.

Degree: 2011, Technical University of Lisbon

Mestrado em Engenharia Agronómica - Protecção das Plantas - Instituto Superior de Agronomia

Phytoplasmas are unicellular organisms, prokaryotes, cell wall-less that cause diseases in hundreds vegetable species. This study is mainly about the prospection of “Candidatus” phytoplasma pyri, “Candidatus” phytoplasma mali and “Candidatus” phytoplasma prunorum, etiologic agents of PD, AP e ESFY, vectored by psyllids Cacopsylla pyri, Cacopsylla picta e Cacopsylla pruni, respectively. These three phytoplasmas belong to AP or 16SrX group, considered quarantine organisms (A2 list, EPPO). In this study, two DNA extraction methods as well as two identification methods using PCR were compared. The two extraction methods differ in lyses buffer which seems to be crucial to obtain high quality DNA. The most used identification method until recently is nested-PCR but as it is a time consuming and expensive method, it’s being replaced by realtime PCR a quicker, more viable and less expensive one, as it was conf irmed in this study. Plant and insect samples from pome and prunus fruit trees in Oeste area were analysed. With major importance lies the fact that, for the first time, we report the presence of “Candidatus” phytoplasma pyri in insect and plant samples.

Advisors/Committee Members: Sousa, Esmeraldina Agostinho Nascimento, Mexia, António Maria Marques.

Subjects/Keywords: phytoplasmas; 16SrX group; psyllids; nested-PCR; real-time PCR; Candidatus; phytoplasma pyri

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APA (6th Edition):

Marques, A. I. A. (2011). Pesquisa de fitoplasmas das pomóideas e prunóideas em Portugal. (Thesis). Technical University of Lisbon. Retrieved from http://www.rcaap.pt/detail.jsp?id=oai:www.repository.utl.pt:10400.5/4117

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16th Edition):

Marques, Ana Isabel Ambrósio. “Pesquisa de fitoplasmas das pomóideas e prunóideas em Portugal.” 2011. Thesis, Technical University of Lisbon. Accessed December 15, 2019. http://www.rcaap.pt/detail.jsp?id=oai:www.repository.utl.pt:10400.5/4117.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7th Edition):

Marques, Ana Isabel Ambrósio. “Pesquisa de fitoplasmas das pomóideas e prunóideas em Portugal.” 2011. Web. 15 Dec 2019.

Vancouver:

Marques AIA. Pesquisa de fitoplasmas das pomóideas e prunóideas em Portugal. [Internet] [Thesis]. Technical University of Lisbon; 2011. [cited 2019 Dec 15]. Available from: http://www.rcaap.pt/detail.jsp?id=oai:www.repository.utl.pt:10400.5/4117.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Marques AIA. Pesquisa de fitoplasmas das pomóideas e prunóideas em Portugal. [Thesis]. Technical University of Lisbon; 2011. Available from: http://www.rcaap.pt/detail.jsp?id=oai:www.repository.utl.pt:10400.5/4117

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

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