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1. Fath, Puria Motamed. Construction of a Copper Bioreporter Screening, characterization and genetic improvement of copper-sensitive bacteria.

Degree: Engineering, 2010, University of Borås

In the nature, lots of organism apply different kinds of lights such as flourscence or luminoscence for some purposes such as defence or hunting. Firefly luciferase and Bacterial luciferase are the most famous ones which have been used to design Biosensors or Bioreporters in recent decades. Their applications are so extensive from detecting pollutions in the environment to medical and treatment usages. To design Copper Bioreporter, copper resistance promoter from COP operon which plays an important role in Pseudomonas syringae and pGL3 plasmid which has luciferase gene were utilized. To achieve that target, sequences of promoter were synthesized and inserted to pCR2.1 vector, then suitable primers with considering restriction sites were designed to get high concentration of DNA. After digestion of pGL3 and interested gene by Nhe I and Sac I enzymes, ligation was performed, and then recombinat plasmids were transferred to E. coli BL-21 as a host cell. Finallay, luciferase assay of designed bioreporter was performed by Luminometer in presence of different concentration of CuSO4. The result was maginificant that confirmed design of Copper Bioreporter.

Subjects/Keywords: copper bioreporter; luciferase assay; cop operon; pgl3; e. coli bl-21; Engineering and Technology; Teknik och teknologier

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6th Edition):

Fath, P. M. (2010). Construction of a Copper Bioreporter Screening, characterization and genetic improvement of copper-sensitive bacteria. (Thesis). University of Borås. Retrieved from http://urn.kb.se/resolve?urn=urn:nbn:se:hb:diva-19682

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16th Edition):

Fath, Puria Motamed. “Construction of a Copper Bioreporter Screening, characterization and genetic improvement of copper-sensitive bacteria.” 2010. Thesis, University of Borås. Accessed January 21, 2020. http://urn.kb.se/resolve?urn=urn:nbn:se:hb:diva-19682.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7th Edition):

Fath, Puria Motamed. “Construction of a Copper Bioreporter Screening, characterization and genetic improvement of copper-sensitive bacteria.” 2010. Web. 21 Jan 2020.

Vancouver:

Fath PM. Construction of a Copper Bioreporter Screening, characterization and genetic improvement of copper-sensitive bacteria. [Internet] [Thesis]. University of Borås; 2010. [cited 2020 Jan 21]. Available from: http://urn.kb.se/resolve?urn=urn:nbn:se:hb:diva-19682.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Fath PM. Construction of a Copper Bioreporter Screening, characterization and genetic improvement of copper-sensitive bacteria. [Thesis]. University of Borås; 2010. Available from: http://urn.kb.se/resolve?urn=urn:nbn:se:hb:diva-19682

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

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