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You searched for subject:(perfused liver). Showing records 1 – 2 of 2 total matches.

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Vanderbilt University

1. Miller, Corin O'Dell. Non-invasive Measurements of Hepatic Glycogen Levels and Glycogen Synthesis Rates Using Chemical Exchange Saturation Transfer and Comparison to 13C NMR Spectroscopy.

Degree: PhD, Interdisciplinary Studies: Magnetic Resonance Imaging and Spectroscopic Methodology, 2015, Vanderbilt University

Glycogen is the primary tissue storage form of carbohydrates and plays in integral role in whole body glucose homeostasis, yet remains difficult to measure without invasive biopsies. A novel non-invasive approach to measure liver glycogen using chemical exchange saturation transfer (CEST) was developed and optimized in phantoms, and then applied to isolated perfused mouse livers at 11.7T, and to mice in vivo at 7T. Measurements of total glycogen content and glycogen synthesis rates were performed under various experimental conditions in perfused livers and compared with interleaved measurements using 13C magnetic resonance spectroscopy (MRS). Monte Carlo simulations were used to investigate the correlation between, and the inherent errors in, each measurement. CEST and 13C MRS measurements were highly correlated, with CEST measurements of total glycogen being more precise, and CEST measurements of glycogen synthesis rates being less precise than 13C MRS. This approach was then applied to a novel pharmacological question, the acute effect of AMP-activated protein kinase (AMPK) activation on hepatic glycogen synthesis. In contrast to previous reports of AMPK activation promoting increased glycogen synthesis in muscle, activation of AMPK with the small molecule MK8722 resulted in a decrease in hepatic glycogen synthesis. This CEST approach was then combined with a glucose infusion protocol in mice, and CEST measurements of glycogen synthesis rates were performed in vivo at 7T. Preliminary studies revealed that CEST was capable of detecting increases in glycogen over time, and that high circulating glucose concentrations did not have a large contaminating effect on the glycogen CEST signal. The results of these studies suggest that CEST can be used to accurately measure glycogen levels and glycogen synthesis rates over the physiological range, and lay the groundwork for the translation of this approach into larger animals and humans. Advisors/Committee Members: Eduard Chekmenev (committee member), Bruce Damon (committee member), John Gore (committee member), Alan Cherrington (chair).

Subjects/Keywords: Magnetic resonance; 13C NMR; Glycogen; chemical exchange saturation transfer; CEST; perfused liver

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APA (6th Edition):

Miller, C. O. (2015). Non-invasive Measurements of Hepatic Glycogen Levels and Glycogen Synthesis Rates Using Chemical Exchange Saturation Transfer and Comparison to 13C NMR Spectroscopy. (Doctoral Dissertation). Vanderbilt University. Retrieved from http://etd.library.vanderbilt.edu//available/etd-07112015-081716/ ;

Chicago Manual of Style (16th Edition):

Miller, Corin O'Dell. “Non-invasive Measurements of Hepatic Glycogen Levels and Glycogen Synthesis Rates Using Chemical Exchange Saturation Transfer and Comparison to 13C NMR Spectroscopy.” 2015. Doctoral Dissertation, Vanderbilt University. Accessed October 20, 2019. http://etd.library.vanderbilt.edu//available/etd-07112015-081716/ ;.

MLA Handbook (7th Edition):

Miller, Corin O'Dell. “Non-invasive Measurements of Hepatic Glycogen Levels and Glycogen Synthesis Rates Using Chemical Exchange Saturation Transfer and Comparison to 13C NMR Spectroscopy.” 2015. Web. 20 Oct 2019.

Vancouver:

Miller CO. Non-invasive Measurements of Hepatic Glycogen Levels and Glycogen Synthesis Rates Using Chemical Exchange Saturation Transfer and Comparison to 13C NMR Spectroscopy. [Internet] [Doctoral dissertation]. Vanderbilt University; 2015. [cited 2019 Oct 20]. Available from: http://etd.library.vanderbilt.edu//available/etd-07112015-081716/ ;.

Council of Science Editors:

Miller CO. Non-invasive Measurements of Hepatic Glycogen Levels and Glycogen Synthesis Rates Using Chemical Exchange Saturation Transfer and Comparison to 13C NMR Spectroscopy. [Doctoral Dissertation]. Vanderbilt University; 2015. Available from: http://etd.library.vanderbilt.edu//available/etd-07112015-081716/ ;


Kyoto University

2. Tamaki, Ichiro. Hydrogen Flush After Cold Storage (HyFACS), as a new end-ischemic ex vivo treatment for liver grafts against ischemia/reperfusion injury .

Degree: 2019, Kyoto University

Subjects/Keywords: liver transplantation; organ preservation; isolated perfused rat liver; extracorporeal therapy; hydrogen

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6th Edition):

Tamaki, I. (2019). Hydrogen Flush After Cold Storage (HyFACS), as a new end-ischemic ex vivo treatment for liver grafts against ischemia/reperfusion injury . (Thesis). Kyoto University. Retrieved from http://hdl.handle.net/2433/242357

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16th Edition):

Tamaki, Ichiro. “Hydrogen Flush After Cold Storage (HyFACS), as a new end-ischemic ex vivo treatment for liver grafts against ischemia/reperfusion injury .” 2019. Thesis, Kyoto University. Accessed October 20, 2019. http://hdl.handle.net/2433/242357.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7th Edition):

Tamaki, Ichiro. “Hydrogen Flush After Cold Storage (HyFACS), as a new end-ischemic ex vivo treatment for liver grafts against ischemia/reperfusion injury .” 2019. Web. 20 Oct 2019.

Vancouver:

Tamaki I. Hydrogen Flush After Cold Storage (HyFACS), as a new end-ischemic ex vivo treatment for liver grafts against ischemia/reperfusion injury . [Internet] [Thesis]. Kyoto University; 2019. [cited 2019 Oct 20]. Available from: http://hdl.handle.net/2433/242357.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Tamaki I. Hydrogen Flush After Cold Storage (HyFACS), as a new end-ischemic ex vivo treatment for liver grafts against ischemia/reperfusion injury . [Thesis]. Kyoto University; 2019. Available from: http://hdl.handle.net/2433/242357

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

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