You searched for subject:(peptide).
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University of Minnesota
1.
Scott, Carolyn.
Peptide-functionalized hydrogels for three-dimensional cell culture.
Degree: PhD, Biomedical Engineering, 2016, University of Minnesota
URL: http://hdl.handle.net/11299/185633 
► Biomimetic scaffolds have played a major role in the advancements in tissue engineering. In addition to mimicking the stiffness, nanofibrous structure, and biochemistry of the…
(more)
▼ Biomimetic scaffolds have played a major role in the advancements in tissue engineering. In addition to mimicking the stiffness, nanofibrous structure, and biochemistry of the native extracellular matrix (ECM), reproducing the three-dimensional (3D) environment of the ECM has been shown to be extremely important. To this end, we designed a co-assembling peptide-amphiphile hydrogel system containing the fibronectin-mimetic PR_g peptide-amphiphile and an E2 diluent peptide-amphiphile for the entrapment and culture of cells in 3D. The E2 diluent peptide amphiphile was designed to screen charges on the PR_g and increase the kinetics of self-assembly in physiologically relevant solutions. Our study investigated two weight percent formulations, 0.5 and 1.0 wt %, and found that in both, entrapped fibroblasts survived encapsulation, proliferated, and deposited collagen IV and fibronectin ECM proteins. The 0.5 wt % gels had a modulus of 429 Pa and supported significantly more fibroblasts proliferation than the 1.0 wt % gels, which had a modulus of 809 Pa. The 1.0 wt% gels though, supported significantly higher mRNA expression and production of ECM proteins. This result indicates by tuning the wt % of our peptide-amphiphile hydrogels, we can encourage either rapid proliferation or ECM deposition. While peptide-amphiphiles are an attractive material for the design of cell scaffolds due to their ability to self-assemble into nanofibrous hydrogels and incorporate multiple biomimetic peptides, there are some limitations associated with these physical hydrogels. One such limitation is that the kinetics of assembly and the mechanical properties of the resulting hydrogels are dependent upon the peptide sequence. We found that the modulus of multifunctional peptide-amphiphile hydrogels ranged from 1000 to 6500 Pa, which was too broad a range to deconvolute the effect of the peptide signal and the effect of mechanical properties. To simplify our system and study the effects of combining ECM protein-mimetic and growth factor-mimetic peptides on the proliferation and function of pancreatic β-cells, peptide mimetics were covalently immobilized on plates. This study found that β-cells proliferate more and secreted significantly more insulin on peptide-functionalized compared to non-functionalized controls. The specific peptide mimetic or combination of peptide mimetics did not significantly affect insulin secretions, but literature suggests that other signals, including cell-cell signaling may play a more significant role in insulin secretion than ECM protein or growth factor signaling. A poly(ethylene glycol) dimethacrylate (PEGDM) hydrogel was functionalized with the laminin-mimetic IKVAV peptide at a 20 µM concentration to match the modulus of non-functionalized hydrogels. This system was used to determine if peptide-functionalization also had an effect in 3D. We found β-cells in IKVAV-functionalized hydrogels proliferated significantly more than β-cells in non-functionalized scaffolds, but no differences in insulin secretion…
Subjects/Keywords: biomaterials; hydrogel; peptide; peptide-amphiphile
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APA (6th Edition):
Scott, C. (2016). Peptide-functionalized hydrogels for three-dimensional cell culture. (Doctoral Dissertation). University of Minnesota. Retrieved from http://hdl.handle.net/11299/185633
Chicago Manual of Style (16th Edition):
Scott, Carolyn. “Peptide-functionalized hydrogels for three-dimensional cell culture.” 2016. Doctoral Dissertation, University of Minnesota. Accessed September 19, 2019.
http://hdl.handle.net/11299/185633.
MLA Handbook (7th Edition):
Scott, Carolyn. “Peptide-functionalized hydrogels for three-dimensional cell culture.” 2016. Web. 19 Sep 2019.
Vancouver:
Scott C. Peptide-functionalized hydrogels for three-dimensional cell culture. [Internet] [Doctoral dissertation]. University of Minnesota; 2016. [cited 2019 Sep 19].
Available from: http://hdl.handle.net/11299/185633.
Council of Science Editors:
Scott C. Peptide-functionalized hydrogels for three-dimensional cell culture. [Doctoral Dissertation]. University of Minnesota; 2016. Available from: http://hdl.handle.net/11299/185633
University of Georgia
2.
Walker, Jennifer Renee.
Improving the stability of bioactive peptides using protein-based motifs.
Degree: PhD, Microbiology, 2002, University of Georgia
URL: http://purl.galileo.usg.edu/uga_etd/walker_jennifer_r_200212_phd
► Peptide instability and poor delivery in humans hinders the development of effective peptide drugs. To search for novel protective motifs for stabilizing peptides, an in…
(more)
▼ Peptide instability and poor delivery in humans hinders the development of effective
peptide drugs. To search for novel protective motifs for stabilizing peptides, an in vivo screen of randomized inhibitor peptides was developed and two protective motifs were identified. The first motif was generated by the fusion of the inhibitory peptides to the small stable protein, Rop. The second motif was generated by the placement of one or more prolines at the terminal ends of peptides. Repeating the in vivo screen with random peptides fused to either Rop or encoding terminal proline residues revealed an increase in the frequency of identifiable inhibitor peptides. Next, an in vitro method was developed to further investigate the novel proline protective motif. Randomized synthetic peptides beginning and ending with one proline, two proline or an alanine and two proline residues were tested for increased half-life in various eukaryotic and prokaryotic cell extracts. Peptides protected at the amino terminus with an alanine and two proline residues (APP) exhibited the longest half-lives. Molecular modeling methods suggested that the APP motif exhibited two possible conformers with one possessing two right angles along the
peptide backbone which may cause steric hindrance at the terminus. This increased steric strain within the APP motif may be responsible for increased resistance to peptidases. Then, APP was modified in search for more protective motifs. The alanine residue was replaced with amino acids having an increased tendency to form a cis bond, which has been proven to resist some peptidases. Five modified motifs were tested for increased
peptide half-life in rat serum. The three proline residues (PPP) substituted motif displayed the longest half-life. This motif may provide a level of protection surpassing that of other known protective motifs. While extended stability is one of many factors in
peptide efficacy, uptake by the target cell is also important. A discovery was made that indicated biotinylated peptides could be transported across the membranes of Gram-negative bacteria via the biotin uptake pathway. Biotinylation may thus provide an alternative pathway for antibiotic
peptide uptake.
Advisors/Committee Members: Juergen Wiegel.
Subjects/Keywords: peptide
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APA (6th Edition):
Walker, J. R. (2002). Improving the stability of bioactive peptides using protein-based motifs. (Doctoral Dissertation). University of Georgia. Retrieved from http://purl.galileo.usg.edu/uga_etd/walker_jennifer_r_200212_phd
Chicago Manual of Style (16th Edition):
Walker, Jennifer Renee. “Improving the stability of bioactive peptides using protein-based motifs.” 2002. Doctoral Dissertation, University of Georgia. Accessed September 19, 2019.
http://purl.galileo.usg.edu/uga_etd/walker_jennifer_r_200212_phd.
MLA Handbook (7th Edition):
Walker, Jennifer Renee. “Improving the stability of bioactive peptides using protein-based motifs.” 2002. Web. 19 Sep 2019.
Vancouver:
Walker JR. Improving the stability of bioactive peptides using protein-based motifs. [Internet] [Doctoral dissertation]. University of Georgia; 2002. [cited 2019 Sep 19].
Available from: http://purl.galileo.usg.edu/uga_etd/walker_jennifer_r_200212_phd.
Council of Science Editors:
Walker JR. Improving the stability of bioactive peptides using protein-based motifs. [Doctoral Dissertation]. University of Georgia; 2002. Available from: http://purl.galileo.usg.edu/uga_etd/walker_jennifer_r_200212_phd
University of Georgia
3.
Warren, James Walter.
A molecular genetic approach to stabilizing bioactive peptides via protein-based motifs.
Degree: PhD, Microbiology, 2004, University of Georgia
URL: http://purl.galileo.usg.edu/uga_etd/warren_james_w_200408_phd
► Instability of bioactive peptides represents a major challenge to the development of these molecules as drugs. The purpose of this study was the investigation of…
(more)
▼ Instability of bioactive peptides represents a major challenge to the development of these molecules as drugs. The purpose of this study was the investigation of protein-based motifs and their potential for protecting peptides from enzymatic degradation. A novel, highly regulable expression vector, pLAC11, was constructed so that potent inhibitory peptides could be isolated from an in vivo genetic screen designed to generate randomized bioactive peptides that inhibit the growth of bacteria. Compared to other commonly utilized expression vectors that are known not to be tightly regulable, pLAC11 was demonstrated to possess the ability to regulate potent inhibitor peptides which could prove lethal to the cells generating them. The three protein-based motifs that were examined by this screen were the fusion of randomized peptides to the highly stable Rop protein, the generation of !-helical peptides via the random incorporation of a set of hydrophilic helix-forming amino acids, and the use of oppositely charged residues at the termini of randomized peptides. All three methods were observed to yield an increase in the frequency at which potent inhibitors were isolated during the in vivo screen as compared to unprotected peptides, presumably due to greater stability. To further investigate the possible role of secondary structure in
peptide stability, a group of !-helical peptides based on Rop and other helical proteins as well as putative helical peptides designed de novo were examined by secondary structure prediction algorithms, CD spectroscopy, and in vitro plasma degradation assays. Two highly stable helical peptides were identified and found to have half-lives much greater than other representative peptides and similar to that of small stable proteins.
Advisors/Committee Members: Timothy Hoover.
Subjects/Keywords: Peptide
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APA (6th Edition):
Warren, J. W. (2004). A molecular genetic approach to stabilizing bioactive peptides via protein-based motifs. (Doctoral Dissertation). University of Georgia. Retrieved from http://purl.galileo.usg.edu/uga_etd/warren_james_w_200408_phd
Chicago Manual of Style (16th Edition):
Warren, James Walter. “A molecular genetic approach to stabilizing bioactive peptides via protein-based motifs.” 2004. Doctoral Dissertation, University of Georgia. Accessed September 19, 2019.
http://purl.galileo.usg.edu/uga_etd/warren_james_w_200408_phd.
MLA Handbook (7th Edition):
Warren, James Walter. “A molecular genetic approach to stabilizing bioactive peptides via protein-based motifs.” 2004. Web. 19 Sep 2019.
Vancouver:
Warren JW. A molecular genetic approach to stabilizing bioactive peptides via protein-based motifs. [Internet] [Doctoral dissertation]. University of Georgia; 2004. [cited 2019 Sep 19].
Available from: http://purl.galileo.usg.edu/uga_etd/warren_james_w_200408_phd.
Council of Science Editors:
Warren JW. A molecular genetic approach to stabilizing bioactive peptides via protein-based motifs. [Doctoral Dissertation]. University of Georgia; 2004. Available from: http://purl.galileo.usg.edu/uga_etd/warren_james_w_200408_phd
4.
Cabalteja, Chino Cabasa.
Bioengineering alpha conotoxin ViI : from disulfide bonds to native chemical ligation.
Degree: 2015, University of Hawaii – Manoa
URL: http://hdl.handle.net/10125/100541
► M.S. University of Hawaii at Manoa 2014.
The paradigm of rational drug design has broadened its focus from classical small-molecule drugs and into larger biological…
(more)
▼ M.S. University of Hawaii at Manoa 2014.
The paradigm of rational drug design has broadened its focus from classical small-molecule drugs and into larger biological molecules (biologics). At the forefront of this change are peptide drugs with high potency, selectivity and efficacy toward a broader range of targets. Conotoxins isolated from the venom of marine cone snails have received much attention because they are highly specific and isoform selective towards their target receptors, making them ideal resources for potential drug leads.
Members of the α-conotoxin family have been extensively studied because they are competitive antagonists of nicotinic acetylcholine receptors (nAChRs) located in the central and peripheral nervous systems. Modulation of these receptors could aid in the control of pain and other associated pathologies. The characteristic receptor-ligand interaction of these peptides is contingent on their well-defined tertiary structure, which is stabilized by several disulfide bonds. In the venom duct, native α-conotoxins enzymatically fold into the globular conformation. However, permutation of the disulfide bond connections of α-conotoxins can theoretically yield three conformations/isomers: globular, ribbon, and beaded. In vitro studies on conotoxin folding have suggested that post-translational modifications (PTM) such as hydroxylated prolines aid in the folding of conotoxins. As such, it was hypothesized that elimination of this PTM would increase the likelihood of peptide isomer formation.
To investigate this connection, the novel peptide, α-conotoxin ViI from Conus virgo was utilized as it contained two hydroxyprolines at position 6 and 13 in its sequence. α-Conotoxin ViI and its non-post-translationally modified variant Vi1.1 were synthesized via fluorenylmethoxycarbonyl solid phase peptide synthesis. Random oxidation of Vi1.1 revealed two products of identical mass later identified as the ribbon and globular isomers. These isomers exhibited a degree of nAChR inhibition in a bovine chromaffin cell assay. Subsequently, both α-conotoxin ViI and Vi1.1 were allowed to oxidize in buffers containing chaotropic agents. It was found that oxidation of Vi1.1in various chaotropic agents increased the creation of non-native isomer as compared to oxidation in ammonium bicarbonate. Furthermore, oxidations of α-conotoxin ViI revealed that some portion of the peptides remained in the reduced form. This work provides an initial investigation for a long-term study on the connection between the generation of disulfide bond isomers and hydroxyprolines.
Efforts were also undertaken to connect the N-and C-terminal peptide fragments of the spider toxin Huwentoxin I via native chemical ligation. Assembly of the complete linear sequence of Huwentoxin I was subsequently verified through electrospray ionization mass spectrometry. This work will be utilized to create an intramolecular native chemical ligation strategy for the cyclization of α-conotoxin ViI and its analogues. Like their linear counterparts, these…
Subjects/Keywords: peptide isomers
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APA (6th Edition):
Cabalteja, C. C. (2015). Bioengineering alpha conotoxin ViI : from disulfide bonds to native chemical ligation. (Thesis). University of Hawaii – Manoa. Retrieved from http://hdl.handle.net/10125/100541
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Cabalteja, Chino Cabasa. “Bioengineering alpha conotoxin ViI : from disulfide bonds to native chemical ligation.” 2015. Thesis, University of Hawaii – Manoa. Accessed September 19, 2019.
http://hdl.handle.net/10125/100541.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Cabalteja, Chino Cabasa. “Bioengineering alpha conotoxin ViI : from disulfide bonds to native chemical ligation.” 2015. Web. 19 Sep 2019.
Vancouver:
Cabalteja CC. Bioengineering alpha conotoxin ViI : from disulfide bonds to native chemical ligation. [Internet] [Thesis]. University of Hawaii – Manoa; 2015. [cited 2019 Sep 19].
Available from: http://hdl.handle.net/10125/100541.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Cabalteja CC. Bioengineering alpha conotoxin ViI : from disulfide bonds to native chemical ligation. [Thesis]. University of Hawaii – Manoa; 2015. Available from: http://hdl.handle.net/10125/100541
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
University of Hong Kong
5.
鲁尧; Lu, Yao.
The study of cytotoxicity and membrane interaction of
D-LAK antimicrobial peptides.
Degree: Master of Medical Sciences, 2015, University of Hong Kong
URL: Lu,
Y.
[鲁尧].
(2015).
The
study
of
cytotoxicity
and
membrane
interaction
of
D-LAK
antimicrobial
peptides.
(Thesis).
University
of
Hong
Kong,
Pokfulam,
Hong
Kong
SAR.
Retrieved
from
http://dx.doi.org/10.5353/th_b5635922
;
http://hdl.handle.net/10722/221472
► Tuberculosis (TB) remains a major global heath problem. Mismanagement of TB leads to the development of multidrug-resistant tuberculosis (MDR-TB). There is an urgent need to…
(more)
▼ Tuberculosis (TB) remains a major global heath
problem. Mismanagement of TB leads to the development of
multidrug-resistant tuberculosis (MDR-TB). There is an urgent need
to develop new and effective therapeutics against MDR-TB.
Antimicrobial peptides (AMPs) have emerged as a new class of
anti-TB agent against drug-resistant TB. AMPs are an essential
component of the innate immune system that defend against invading
pathogens. AMPs exhibit broad-spectrum antimicrobial activity
against a wide range of microorganisms. It is interesting to
determine the therapeutic potential of AMPs against MDR-TB for
clinical use. This study aimed to study the cytotoxicity of D-LAK
AMPs and their combinations with anti-TB drugs on THP-1 cells and
HEP G2 cells and to investigate the cell membrane interaction of
D-LAK peptides. MTT assay was performed to determine the
cytotoxicity of D-LAK120-A, D-LAK120-HP13, isoniazid (INH) and
rifampicin (RIF) on THP-1 cells and HEP G2 cells. The cytotoxicity
of the combinations of D-AMPs/anti-TB drugs (D-LAK120-A/INH,
D-LAK120-A/RIF, D-LAK120-HP13/INH andD-LAK120-HP13/RIF) on THP-1
cells and HEP G2 cells were also studied using MTT assay to
determine the combination effect of D-AMPs with anti-TB drugs. IC50
values were obtained from MTT assay to determine the concentrations
at which these agents were safe for use. A fluorescent dye,
calcein-AM, was used in cell-membrane interaction studies. Flow
cytometry and confocal microscope experiments were performed to
elucidate the mechanism of membrane activity of D-LAK120-A and
D-LAK120-HP13. From the two cell membrane interaction studies, it
was found that D-LAK120-A and D-LAK120-HP13 had interactions on the
cell surface of THP-1 cells. There was an obvious cytotoxic effect
at concentration of 16 μM and above for both peptides on THP-1
cells in the cell membrane interaction study. Future studies will
be focused on the anti-TB effects of these peptides in combination
with anti-TB drugs on MDR-TB at their non-toxic
concentrations.
published_or_final_version
Pharmacology and Pharmacy
Master
Master of Medical Sciences
Subjects/Keywords: Peptide antibiotics
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APA ·
Chicago ·
MLA ·
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CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
鲁尧; Lu, Y. (2015). The study of cytotoxicity and membrane interaction of
D-LAK antimicrobial peptides. (Masters Thesis). University of Hong Kong. Retrieved from Lu, Y. [鲁尧]. (2015). The study of cytotoxicity and membrane interaction of D-LAK antimicrobial peptides. (Thesis). University of Hong Kong, Pokfulam, Hong Kong SAR. Retrieved from http://dx.doi.org/10.5353/th_b5635922 ; http://hdl.handle.net/10722/221472
Chicago Manual of Style (16th Edition):
鲁尧; Lu, Yao. “The study of cytotoxicity and membrane interaction of
D-LAK antimicrobial peptides.” 2015. Masters Thesis, University of Hong Kong. Accessed September 19, 2019.
Lu, Y. [鲁尧]. (2015). The study of cytotoxicity and membrane interaction of D-LAK antimicrobial peptides. (Thesis). University of Hong Kong, Pokfulam, Hong Kong SAR. Retrieved from http://dx.doi.org/10.5353/th_b5635922 ; http://hdl.handle.net/10722/221472.
MLA Handbook (7th Edition):
鲁尧; Lu, Yao. “The study of cytotoxicity and membrane interaction of
D-LAK antimicrobial peptides.” 2015. Web. 19 Sep 2019.
Vancouver:
鲁尧; Lu Y. The study of cytotoxicity and membrane interaction of
D-LAK antimicrobial peptides. [Internet] [Masters thesis]. University of Hong Kong; 2015. [cited 2019 Sep 19].
Available from: Lu, Y. [鲁尧]. (2015). The study of cytotoxicity and membrane interaction of D-LAK antimicrobial peptides. (Thesis). University of Hong Kong, Pokfulam, Hong Kong SAR. Retrieved from http://dx.doi.org/10.5353/th_b5635922 ; http://hdl.handle.net/10722/221472.
Council of Science Editors:
鲁尧; Lu Y. The study of cytotoxicity and membrane interaction of
D-LAK antimicrobial peptides. [Masters Thesis]. University of Hong Kong; 2015. Available from: Lu, Y. [鲁尧]. (2015). The study of cytotoxicity and membrane interaction of D-LAK antimicrobial peptides. (Thesis). University of Hong Kong, Pokfulam, Hong Kong SAR. Retrieved from http://dx.doi.org/10.5353/th_b5635922 ; http://hdl.handle.net/10722/221472
Wake Forest University
6.
Altamimi, Afnan M.
TESTING THE EFFECT OF A NOVEL HYDROGEN SULFIDE RELEASING PEPTIDE ON INFECTED BURN WOUNDS.
Degree: 2019, Wake Forest University
URL: http://hdl.handle.net/10339/93900
► Burn wounds are a devastating form of injury that leads to substantial morbidity, mortality, and cost. One of the critical complications of burn wounds is…
(more)
▼ Burn wounds are a devastating form of injury that leads to substantial morbidity, mortality, and cost. One of the critical complications of burn wounds is infections, especially with Staphylococcus aureus. Rising antimicrobial resistance is contributing to the complexity of wound management. According to the CDC, each year in the U.S. at least 2 million people are diagnosed with antibiotic-resistant bacteria, and more than 20,000 people die as a result.
Subjects/Keywords: Antimicrobial Peptide
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APA ·
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APA (6th Edition):
Altamimi, A. M. (2019). TESTING THE EFFECT OF A NOVEL HYDROGEN SULFIDE RELEASING PEPTIDE ON INFECTED BURN WOUNDS. (Thesis). Wake Forest University. Retrieved from http://hdl.handle.net/10339/93900
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Altamimi, Afnan M. “TESTING THE EFFECT OF A NOVEL HYDROGEN SULFIDE RELEASING PEPTIDE ON INFECTED BURN WOUNDS.” 2019. Thesis, Wake Forest University. Accessed September 19, 2019.
http://hdl.handle.net/10339/93900.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Altamimi, Afnan M. “TESTING THE EFFECT OF A NOVEL HYDROGEN SULFIDE RELEASING PEPTIDE ON INFECTED BURN WOUNDS.” 2019. Web. 19 Sep 2019.
Vancouver:
Altamimi AM. TESTING THE EFFECT OF A NOVEL HYDROGEN SULFIDE RELEASING PEPTIDE ON INFECTED BURN WOUNDS. [Internet] [Thesis]. Wake Forest University; 2019. [cited 2019 Sep 19].
Available from: http://hdl.handle.net/10339/93900.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Altamimi AM. TESTING THE EFFECT OF A NOVEL HYDROGEN SULFIDE RELEASING PEPTIDE ON INFECTED BURN WOUNDS. [Thesis]. Wake Forest University; 2019. Available from: http://hdl.handle.net/10339/93900
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Université de Neuchâtel
7.
Montandon, Cyrille.
Identification and characterization of putative Toc159
interacting partners.
Degree: 2015, Université de Neuchâtel
URL: http://doc.rero.ch/record/257854
► Le chloroplaste est l’organelle qui caractérise les plantes terrestres ainsi que les autres eucaryotes photosynthétiques. Il remplit diverses fonctions métaboliques, mais la photosynthèse est son…
(more)
▼ Le chloroplaste est l’organelle qui caractérise les
plantes terrestres ainsi que les autres eucaryotes
photosynthétiques. Il remplit diverses fonctions métaboliques, mais
la photosynthèse est son activité principale, sa structure et sa
composition protéique en témoignent. Le chloroplaste est le
résultat d’une endosymbiose entre un eucaryote ancestral et une
cyanobactérie photosynthétique. Le génome du chloroplaste, vestige
de son ancienne autonomie, encode environ une centaine de
protéines. Les 2000-3000 autres protéines présentes dans le
chloroplaste sont codées dans le noyau et traduites dans le cytosol
et doivent donc être importées dans le chloroplaste. Le « transit
peptide » (
peptide de transit), une courte séquence à la
terminaison aminée des protéines destinées au chloroplaste, est
suffisant et nécessaire pour l’import spécifique dans le
chloroplaste. La voie Toc/Tic (Translocon at the Outer/Inner
membrane of the chloroplast envelope: « Translocon à la membrane
externe/interne de l’enveloppe du chloroplaste ») reconnait et
import ces protéines en présence d’ATP et de GTP. Le noyau du
complexe Toc est composé de Toc159 et Toc34/33, 2 récepteurs
possédant un domaine GTPase, et de Toc75 qui constitue le pore du
complexe. Toc159 est formé de 3 domaines, un domaine ancrant la
protéine dans la membrane à l’extrémité carboxyle (domaine M), un
domaine GTPase (domaine G) et un domaine acide (domaine A). Le taux
d’import des protéines et sa spécificité envers les protéines
clientes varient en fonction du développement de la plante, du
tissu ou du type de plaste. Les différents homologues de Toc159 et
de Toc34/33 chez <i>A. thaliana</i> forment des
complexes distincts. Ils montrent une spécificité d’import
différente et leur expression varie en fonction du stade de
développement ou de la partie de la plante. Le domaine A de Toc159
et de ses homologues détermine partiellement la spécificité envers
les différents types de protéines clientes. Le domaine A est
hyper-phosphorylé et existe sous une forme soluble, certainement le
résultat d’un clivage spécifique. Ces modifications
post-traductionnelles pourraient influencer la spécificité de
Toc159 envers les protéines clientes. Le contrôle de l’activité
GTPase de Toc159 ou de Toc34/33 pourrait également influencer
l’activité d’import. Le but de ce travail de thèse était la
caractérisation de protéines co-précipitées avec une version taguée
de Toc159 et identifiées par spectrométrie de masse, qui pourraient
être potentiellement responsable des modifications mentionnées
ci-dessus. L’effort principal a été fait sur Emb2004/AT1G10510, une
protéine LRR (Leucine Rich Repeat) et des contributions ont été
faites à la caractérisation d’une protéine kinase
potentielle/AT4G32250 et de Tic56/AT5G01590.
Advisors/Committee Members: Felix (Dir.).
Subjects/Keywords: transit peptide
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APA ·
Chicago ·
MLA ·
Vancouver ·
CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Montandon, C. (2015). Identification and characterization of putative Toc159
interacting partners. (Thesis). Université de Neuchâtel. Retrieved from http://doc.rero.ch/record/257854
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Montandon, Cyrille. “Identification and characterization of putative Toc159
interacting partners.” 2015. Thesis, Université de Neuchâtel. Accessed September 19, 2019.
http://doc.rero.ch/record/257854.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Montandon, Cyrille. “Identification and characterization of putative Toc159
interacting partners.” 2015. Web. 19 Sep 2019.
Vancouver:
Montandon C. Identification and characterization of putative Toc159
interacting partners. [Internet] [Thesis]. Université de Neuchâtel; 2015. [cited 2019 Sep 19].
Available from: http://doc.rero.ch/record/257854.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Montandon C. Identification and characterization of putative Toc159
interacting partners. [Thesis]. Université de Neuchâtel; 2015. Available from: http://doc.rero.ch/record/257854
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
University of Sydney
8.
Wang, Xiaoyi.
Development and Application of Novel Methods for the Synthesis of Modified Proteins
.
Degree: 2018, University of Sydney
URL: http://hdl.handle.net/2123/19892
► Proteins are essential biomolecules that mediate a range of biological processes in humans. Many proteins contain post-translational modifications (PTMs) that are often vital to their…
(more)
▼ Proteins are essential biomolecules that mediate a range of biological processes in humans. Many proteins contain post-translational modifications (PTMs) that are often vital to their biological activity and these modified proteins have recently attracted significant attention as promising therapeutics for the treatment of a range of diseases. Currently, most protein therapeutics are expressed as mixtures of isoforms containing different PTMs, which have a spectrum of biological activities. Understanding the importance and function of individual PTMs requires access to homogeneous protein isoforms, which can often only be achieved by chemical synthesis. Solid phase peptide synthesis (SPPS) combined with native chemical ligation (NCL)- desulfurization chemistry has led to the synthesis of a large number of protein targets with defined structure and function. In recent years, a new methodology called the diselenide-selenoester ligation (DSL) has proven to be a powerful complementary technology to NCL for the ligation of unprotected fragments to create native amide bonds. However, DSL technology, coupled with deselenization chemistry, is currently limited to ligation at alanine junctions due to a lack of availability of β-selenoamino acids other than selenocysteine (Sec, U). The overarching goal of the research described in this thesis therefore is to expand the utility of DSL through the generation of new β-selenoamino acid building blocks, and to validate their use in DSL through the construction of therapeutic protein targets. By enabling access to two extra ligation junctions (leucine and phenylalanine) for the expedient construction of complex post-translationally modified proteins by DSL-deselenization chemistry, this thesis has made important contributions to the area of protein science.
Subjects/Keywords: peptide ligation
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APA (6th Edition):
Wang, X. (2018). Development and Application of Novel Methods for the Synthesis of Modified Proteins
. (Thesis). University of Sydney. Retrieved from http://hdl.handle.net/2123/19892
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Wang, Xiaoyi. “Development and Application of Novel Methods for the Synthesis of Modified Proteins
.” 2018. Thesis, University of Sydney. Accessed September 19, 2019.
http://hdl.handle.net/2123/19892.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Wang, Xiaoyi. “Development and Application of Novel Methods for the Synthesis of Modified Proteins
.” 2018. Web. 19 Sep 2019.
Vancouver:
Wang X. Development and Application of Novel Methods for the Synthesis of Modified Proteins
. [Internet] [Thesis]. University of Sydney; 2018. [cited 2019 Sep 19].
Available from: http://hdl.handle.net/2123/19892.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Wang X. Development and Application of Novel Methods for the Synthesis of Modified Proteins
. [Thesis]. University of Sydney; 2018. Available from: http://hdl.handle.net/2123/19892
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
University of Manchester
9.
Arthanari, Yamini.
Study of cellular delivery of siRNA and shRNA targeting
bcr-abl in chronic myeloid leukemia using Tat derived
peptide.
Degree: 2011, University of Manchester
URL: http://www.manchester.ac.uk/escholar/uk-ac-man-scw:119786
► Chronic Myeloid Leukemia is characterised by the formation of a fusion gene bcr-abl. The gene product BCR-ABL has deregulated tyrosine kinase activity that plays a…
(more)
▼ Chronic Myeloid Leukemia is characterised by the
formation of a fusion gene bcr-abl. The gene product BCR-ABL has
deregulated tyrosine kinase activity that plays a direct role in
the pathogenesis of the disease. Recently, use of siRNA in
leukaemic cells has led to effective gene silencing of bcr-abl.
Gene delivery systems like viral vectors, electroporation and lipid
based vectors have showed varying efficiencies but are limited by
their level of toxicity and immunogenicity. Developments in the
field of Cell Penetrating Peptides have shown effective cellular
uptake of nucleic acids and proteins by the CPPs in vitro and in
vivo. Report from our lab has shown the use of CPP Tat along with
membrane active
peptide LK15 to improve the transfection efficiency
of both Tat and LK15 peptides individually. Hence, this study will
focus on the use of Tat-LK15
peptide to study the delivery of siRNA
and shRNA plasmid in K562 cells and observe the BCR-ABL protein
expression. Cellular uptake studies using Tat-LK15 based complexes
of Cy5-labelled DNA and siRNA showed a concentration dependent
uptake leading to increase in percentage transfected cells.
Tat-LK15 based DNA complexes achieved 80% transfected cells (charge
ratio of 2:1) while siRNA complexes resulted in a maximum of 60%
(charge ratio of 3:1). However, Lipofectamine based DNA complexes
did not show a concentration dependent increase in percentage
transfected cells. Interestingly, Tat-LK15 based siRNA complexes
showed a similar level of uptake and percentage transfected cells
as that of Lipofectamine based siRNA complexes. Cellular uptake
studies using confocal microscopy 4 hours post transfection, showed
that when 1µg of DNA was transfected, the labelled DNA was
primarily localised on the cell membrane. Interestingly, using 5µg
of DNA led to increased intracellular localisation of the labelled
DNA, but this observation was not made with Lipofectamine based
complexes. The observation at 24 hours post transfection of
Tat-LK15/labelled DNA complexes was of higher intensity when
compared to that of Lipofectamine based DNA complexes. The reason
for this is however not known. Interestingly, the cellular uptake
profile using siRNA based complexes was different. At 4 hours post
transfection, there was intracellular localisation of labelled
siRNA. 24 hours post transfection, there was diffuse cytoplasmic
localisation using lower concentration of siRNA whereas using
higher concentration led to more high intensity punctate
localisations within the cell. Similar observations were made for
both Tat-LK15 and Lipofectamine based siRNA complexes.Gene
silencing studies of Tat-LK15/shRNA plasmid complex resulted in 80%
reduction in protein levels 96 hours post transfection for higher
concentrations of shRNA plasmid treated. Similar level of reduction
in BCR-ABL was observed with Lipofectamine based complex.
Supporting evidence of reduction in mRNA levels was observed using
qRT-PCR 48 hours post transfection. However, Tat-LK15/shRNA plasmid
complexes led to around 80% of protein reduction…
Advisors/Committee Members: AOJULA, HARMESH HS, DEMONACOS, CONSTANTINOS C, Aojula, Harmesh, Pluen, Alain, Demonacos, Constantinos.
Subjects/Keywords: Cell penetrating peptide; Tat peptide; RNA interference
Record Details
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APA ·
Chicago ·
MLA ·
Vancouver ·
CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Arthanari, Y. (2011). Study of cellular delivery of siRNA and shRNA targeting
bcr-abl in chronic myeloid leukemia using Tat derived
peptide. (Doctoral Dissertation). University of Manchester. Retrieved from http://www.manchester.ac.uk/escholar/uk-ac-man-scw:119786
Chicago Manual of Style (16th Edition):
Arthanari, Yamini. “Study of cellular delivery of siRNA and shRNA targeting
bcr-abl in chronic myeloid leukemia using Tat derived
peptide.” 2011. Doctoral Dissertation, University of Manchester. Accessed September 19, 2019.
http://www.manchester.ac.uk/escholar/uk-ac-man-scw:119786.
MLA Handbook (7th Edition):
Arthanari, Yamini. “Study of cellular delivery of siRNA and shRNA targeting
bcr-abl in chronic myeloid leukemia using Tat derived
peptide.” 2011. Web. 19 Sep 2019.
Vancouver:
Arthanari Y. Study of cellular delivery of siRNA and shRNA targeting
bcr-abl in chronic myeloid leukemia using Tat derived
peptide. [Internet] [Doctoral dissertation]. University of Manchester; 2011. [cited 2019 Sep 19].
Available from: http://www.manchester.ac.uk/escholar/uk-ac-man-scw:119786.
Council of Science Editors:
Arthanari Y. Study of cellular delivery of siRNA and shRNA targeting
bcr-abl in chronic myeloid leukemia using Tat derived
peptide. [Doctoral Dissertation]. University of Manchester; 2011. Available from: http://www.manchester.ac.uk/escholar/uk-ac-man-scw:119786
Brigham Young University
10.
Dallon, Emma Kay.
Exploration of Antimicrobial Activity in Natural Peptides and High-Throughput Discovery of Synthetic Peptides.
Degree: MS, 2018, Brigham Young University
URL: https://scholarsarchive.byu.edu/cgi/viewcontent.cgi?article=8468&context=etd
► Despite many medical advances, antibiotic resistant bacteria increasingly plague the modern world, necessitating discovery of new antibiotics. One area of nature that can provide inspiration…
(more)
▼ Despite many medical advances, antibiotic resistant bacteria increasingly plague the modern world, necessitating discovery of new antibiotics. One area of nature that can provide inspiration for antibiotics is antimicrobial peptides. Many of these peptides exist in nature, with some classes that have not been studied or characterized well. One such class is the defensin-like peptides generated by the plant Medicago truncatula as part of their symbiotic relationship with Sinorhizobium meliloti. Nodule-specific Cysteine Rich (NCR) peptides are defined by the presence of multiple cysteines, and regulate the growth of S. meliloti within plant cells. While some of these NCR peptides have been shown to have antimicrobial properties, hundreds of peptides remain uncharacterized. We have developed an assay for further characterization of these peptides in E. coli. Of the seven peptides that have been tested using this assay, three have exhibited definitive antimicrobial properties against both E. coli and S. meliloti. Additionally, we have developed a system for discovering novel antimicrobial peptides. This platform, called PepSeq, uses the expression of random peptides in E. coli combined with deep sequencing to detect antimicrobial activity. This technology is capable of screening through millions of peptide molecules simultaneously. Using this platform, we have discovered and confirmed six novel antimicrobial peptides, with hundreds of additional predicted antimicrobial peptides. In addition to the peptides we have analyzed using PepSeq, additional peptide scaffolds could be used to discover more potent antimicrobial peptides.
Subjects/Keywords: antimicrobial peptide; NCR peptide; Life Sciences
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APA ·
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MLA ·
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CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Dallon, E. K. (2018). Exploration of Antimicrobial Activity in Natural Peptides and High-Throughput Discovery of Synthetic Peptides. (Masters Thesis). Brigham Young University. Retrieved from https://scholarsarchive.byu.edu/cgi/viewcontent.cgi?article=8468&context=etd
Chicago Manual of Style (16th Edition):
Dallon, Emma Kay. “Exploration of Antimicrobial Activity in Natural Peptides and High-Throughput Discovery of Synthetic Peptides.” 2018. Masters Thesis, Brigham Young University. Accessed September 19, 2019.
https://scholarsarchive.byu.edu/cgi/viewcontent.cgi?article=8468&context=etd.
MLA Handbook (7th Edition):
Dallon, Emma Kay. “Exploration of Antimicrobial Activity in Natural Peptides and High-Throughput Discovery of Synthetic Peptides.” 2018. Web. 19 Sep 2019.
Vancouver:
Dallon EK. Exploration of Antimicrobial Activity in Natural Peptides and High-Throughput Discovery of Synthetic Peptides. [Internet] [Masters thesis]. Brigham Young University; 2018. [cited 2019 Sep 19].
Available from: https://scholarsarchive.byu.edu/cgi/viewcontent.cgi?article=8468&context=etd.
Council of Science Editors:
Dallon EK. Exploration of Antimicrobial Activity in Natural Peptides and High-Throughput Discovery of Synthetic Peptides. [Masters Thesis]. Brigham Young University; 2018. Available from: https://scholarsarchive.byu.edu/cgi/viewcontent.cgi?article=8468&context=etd
University of Georgia
11.
Haley, Jennifer Ayers.
Polymer-peptide hybrids: self-assembly and combinative assembly with DNA.
Degree: PhD, Chemistry, 2011, University of Georgia
URL: http://purl.galileo.usg.edu/uga_etd/haley_jennifer_a_201105_phd
► Polymer-Peptide hybrids are a novel class of macromolecules that combine sophisticated functionality of biopolymers with synthetic versatility of synthetic polymers. Initially developed by biochemist for…
(more)
▼ Polymer-
Peptide hybrids are a novel class of macromolecules that combine sophisticated functionality of biopolymers with synthetic versatility of synthetic polymers. Initially developed by biochemist for bio-medical applications, polymer-
peptide hybrids have recently garnered a lot of attention by material scientists for a diverse range of applications including nanotechnology, photonics as well as traditional bio-medical applications.
In this work, polymer-
peptide hybrids were synthesized utilizing a modular grafting procedure in which Cysteine-containing peptides are “clicked” on the hydrophobic segment of the amphiphilic polymer polybutadiene-block-poly(ethylene oxide) (PBD-b-PEO) via free radical addition of the thiol onto the double bonds. These polymer-
peptide hybrids were then applied in two areas of research: tuning amphiphilic block copolymer self-assemblies with grafted charged peptides and combinative self-assembly with DNA. Grafting short charged peptides onto PBD-b-PEO results in self-assemblies driven by a combination of hydrophobic association, ionic disturbances and H-bonding. Clustering gene-binding peptides, KWKn, onto (PBD-b-PEO) dramatically changes the DNA condensation pathway. By varying the grafting density and
peptide sequence, precise control over the DNA condensation process can be accomplished.
Advisors/Committee Members: Yan Geng.
Subjects/Keywords: Polymer-Peptide Hybrids
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CSE |
Export
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APA (6th Edition):
Haley, J. A. (2011). Polymer-peptide hybrids: self-assembly and combinative assembly with DNA. (Doctoral Dissertation). University of Georgia. Retrieved from http://purl.galileo.usg.edu/uga_etd/haley_jennifer_a_201105_phd
Chicago Manual of Style (16th Edition):
Haley, Jennifer Ayers. “Polymer-peptide hybrids: self-assembly and combinative assembly with DNA.” 2011. Doctoral Dissertation, University of Georgia. Accessed September 19, 2019.
http://purl.galileo.usg.edu/uga_etd/haley_jennifer_a_201105_phd.
MLA Handbook (7th Edition):
Haley, Jennifer Ayers. “Polymer-peptide hybrids: self-assembly and combinative assembly with DNA.” 2011. Web. 19 Sep 2019.
Vancouver:
Haley JA. Polymer-peptide hybrids: self-assembly and combinative assembly with DNA. [Internet] [Doctoral dissertation]. University of Georgia; 2011. [cited 2019 Sep 19].
Available from: http://purl.galileo.usg.edu/uga_etd/haley_jennifer_a_201105_phd.
Council of Science Editors:
Haley JA. Polymer-peptide hybrids: self-assembly and combinative assembly with DNA. [Doctoral Dissertation]. University of Georgia; 2011. Available from: http://purl.galileo.usg.edu/uga_etd/haley_jennifer_a_201105_phd
University of Alberta
12.
Lohans, Christopher T.
Structural Characterization of Bacterial Antimicrobial
Peptides.
Degree: PhD, Department of Chemistry, 2014, University of Alberta
URL: https://era.library.ualberta.ca/files/8g84mn557
► Paenibacillus polymyxa NRRL B-30509, Paenibacillus terrae NRRL B-30644 and P. polymyxa NRRL B-30507 were found to produce several bacteriocins and non-ribosomal peptides. All three strains…
(more)
▼ Paenibacillus polymyxa NRRL B-30509, Paenibacillus
terrae NRRL B-30644 and P. polymyxa NRRL B-30507 were found to
produce several bacteriocins and non-ribosomal peptides. All three
strains produce tridecaptins, non-ribosomal lipopeptides
antimicrobially active against food pathogen Campylobacter jejuni.
Two of these strains also produce polymyxins, lipopeptides also
active against Gram-negative bacteria. Highly cyclized lantibiotics
paenicidins A and B were isolated from P. polymyxa NRRL B-30509 and
P. terrae NRRL B-30644, respectively. The lanthionine (Lan) and
methyllanthionine (MeLan) post-translational modifications of
paenicidin A were characterized by a novel partial desulfurization
strategy, revealing the connectivity of three interlocking
thioether-containing rings. Biosynthetic gene clusters responsible
for the production of the tridecaptins and paenicidins were
identified in the genome sequences of these strains. Carnobacterium
maltaromaticum C2 produces carnolysin, a novel two-component
lantibiotic with homology to enterococcal cytolysin. The
post-translational modifications of carnolysins A1′ and A2′ were
characterized with NMR spectroscopy and tandem mass spectrometry,
revealing the Lan and MeLan bridging patterns. Like cytolysin,
carnolysin contains unusual LL-Lan and LL-MeLan stereoisomers in
the corresponding positions. However, carnolysin also contains
D-alanine and D-aminobutyrate residues not found in cytolysin. The
carnolysin biosynthetic gene cluster was identified in the genome
of C. maltaromaticum C2. Heterologous expression of carnolysin in
Escherichia coli was achieved by expressing the carnolysin
precursor peptides with lantibiotic synthetase CrnM and reductase
CrnJ. Antimicrobially active products were obtained by
proteolytically removing the N-terminal leader sequences of the
carnolysins isolated from C. maltaromaticum C2. These digested
peptides were active against an array of Gram-positive indicator
organisms, while not demonstrating the hemolytic activity
associated with cytolysin at the levels tested. Enterocin 7A and 7B
are leaderless bacteriocins produced by Enterococcus faecalis 710C.
The impact of solvent on the secondary structure of enterocin 7A
was examined by circular dichroism spectroscopy, revealing a high
degree of α-helicity in fully aqueous conditions. The solution
structure of enterocin 7A was solved based on NMR spectroscopic
data, demonstrating that this peptide consists primarily of three
amphiphilic α-helices burying a hydrophobic core. The structures of
enterocins 7A and 7B resembled a region of the circular bacteriocin
carnocyclin A, potentially having implications regarding the mode
of action of these leaderless bacteriocins. The cysteines involved
in the N-terminal disulfide bridge of class IIa bacteriocin
leucocin A were replaced with leucine residues to probe the impact
of this substitution on structure. While this mutant peptide
retained antimicrobial activity, consistent with similar leucocin A
mutants. The solution structure of (C9L,C14L)-leucocin A in…
Subjects/Keywords: peptide; bacteriocin; antimicrobial
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APA ·
Chicago ·
MLA ·
Vancouver ·
CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Lohans, C. T. (2014). Structural Characterization of Bacterial Antimicrobial
Peptides. (Doctoral Dissertation). University of Alberta. Retrieved from https://era.library.ualberta.ca/files/8g84mn557
Chicago Manual of Style (16th Edition):
Lohans, Christopher T. “Structural Characterization of Bacterial Antimicrobial
Peptides.” 2014. Doctoral Dissertation, University of Alberta. Accessed September 19, 2019.
https://era.library.ualberta.ca/files/8g84mn557.
MLA Handbook (7th Edition):
Lohans, Christopher T. “Structural Characterization of Bacterial Antimicrobial
Peptides.” 2014. Web. 19 Sep 2019.
Vancouver:
Lohans CT. Structural Characterization of Bacterial Antimicrobial
Peptides. [Internet] [Doctoral dissertation]. University of Alberta; 2014. [cited 2019 Sep 19].
Available from: https://era.library.ualberta.ca/files/8g84mn557.
Council of Science Editors:
Lohans CT. Structural Characterization of Bacterial Antimicrobial
Peptides. [Doctoral Dissertation]. University of Alberta; 2014. Available from: https://era.library.ualberta.ca/files/8g84mn557
Mahatma Gandhi University
13.
Sasikumar, P G.
Solid phase peptide synthesis using glycerol based cross
linked polymer supports.
Degree: 2010, Mahatma Gandhi University
URL: http://shodhganga.inflibnet.ac.in/handle/10603/302
► An important objective of the modem biochemical research is to understand the molecular basis of the numerous and intricate biological activities of peptides and proteins…
(more)
▼ An important objective of the modem biochemical
research is to understand the molecular basis of the numerous and
intricate biological activities of peptides and proteins and
therefore able to predict and control these activities. The
impc~rtance dramatically increased today because of the explosive
success ofthe gtnome-sequencing project, which revealed hundreds of
thousands of new proteins, but only as predicted sequence data. The
chemical synthesis and elucidation of the biological function of a
predicted protein molecule is thus a challenge of great
significance. The introduction of unnatural chemical group; into
the covalent structure of a
peptide or protein molecule can provide
valuable insight into questions about peptidelprotein functions and
enzyme activity. The most efficient way of introducing these
unnatural amino acids into peptides and small proteins through the
complementary approach of total chemical synthesis is by the
stepwise solid phase
peptide syn1:hesis. The yield and the purity
of these products are depending upon various factors of which the
most important one is the choice of solid support, its mechanical
and chemical stability, swellability and compatibility with a range
of solvents with different polarity. For a successful synthesis,
the reaction should go to completion in each step at a higher rate.
The physicochemic:al incompatibility of the support with the
growing
peptide chain is considered as another major factor that
affects the performance of the resin in polypeptide synthesis. The
design and synthesis of an efficient polymer support depends on the
judicious selection of monomers, their structure and polarity and
the net hydrophobic/l~ydrophilic balance of the polymer. The work.
presented in the thesis describes the development of a new TRPGGDA
cross-linked polystyrene support with a view to approaching the
above mentioned problems. A brief ov':rviea of the key
characteristics of various solid supports and their modific:ation
with suitable linkers, protection strategies, carboxyl activation
reagent:; and the difficulties encountered in SPPS are described in
the first and second chapters. This review aims to give a
comprehensive outline of the recent developments and refinements in
the field of polymer Summary 188 supported polypepticle synthesis
and various factors ~nvolved in optimising the purity and the yield
of the synthetic
peptide. The third chapter describes the synthesis
of the new polymer support, PS-TRPGGDA. This has been designed to
increase the flexibility of the polymer backbone allowicg better
diffusion of reagents through the matrix. The polymer is
synthe5ised by the aqueous free radical suspension polymerisation
of siyrene and TRPGGDA. The resultant polymers have
propyleneglycol-like character, which provides a spacer effect from
the rigid polystyrene core, and the glycerol moiety present in the
cross-linker serves as the growth site for the polypeptide
synthesis. The hydrophillicity of the crosslinker and its content
in the resin determines the…
Advisors/Committee Members: Pillai, V N Rajasekharan.
Subjects/Keywords: Polimers; Peptide Synthesis
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APA ·
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Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Sasikumar, P. G. (2010). Solid phase peptide synthesis using glycerol based cross
linked polymer supports. (Thesis). Mahatma Gandhi University. Retrieved from http://shodhganga.inflibnet.ac.in/handle/10603/302
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Sasikumar, P G. “Solid phase peptide synthesis using glycerol based cross
linked polymer supports.” 2010. Thesis, Mahatma Gandhi University. Accessed September 19, 2019.
http://shodhganga.inflibnet.ac.in/handle/10603/302.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Sasikumar, P G. “Solid phase peptide synthesis using glycerol based cross
linked polymer supports.” 2010. Web. 19 Sep 2019.
Vancouver:
Sasikumar PG. Solid phase peptide synthesis using glycerol based cross
linked polymer supports. [Internet] [Thesis]. Mahatma Gandhi University; 2010. [cited 2019 Sep 19].
Available from: http://shodhganga.inflibnet.ac.in/handle/10603/302.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Sasikumar PG. Solid phase peptide synthesis using glycerol based cross
linked polymer supports. [Thesis]. Mahatma Gandhi University; 2010. Available from: http://shodhganga.inflibnet.ac.in/handle/10603/302
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
University of Aberdeen
14.
Carnapete Alves Meneses, Célia Clarisse.
The development of a facile solution-phase method for the synthesis of peptides.
Degree: 2010, University of Aberdeen
URL: http://digitool.abdn.ac.uk:80/webclient/DeliveryManager?pid=166204
;
http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.540483
► The synthesis of peptides can be considered as the rate-limiting step in the development of peptide-based medicines. Synthetic methods currently available are limited by several…
(more)
▼ The synthesis of peptides can be considered as the rate-limiting step in the development of peptide-based medicines. Synthetic methods currently available are limited by several aspects, including the high cost of production or excessive waste when applied to large-scale synthesis. The development of a new solution-phase synthesis of peptides is described herein. Initial studies focus on the synthesis of α-peptides in the C-terminal direction, though the occurrence of epimerisation during chain elongation shows the limitation of this approach. Attempts to reduce this problem and to gain a better insight into the epimerisation processes involved are described. The unsuccessful application of this initial strategy to the synthesis of β-peptides is also discussed. A new procedure involving the coupling of amino acids or peptide acids with slight excesses of pentafluorophenyl esters in a THF/water solvent mixture in the N-terminal direction is developed and discussed. Contrary to modern repetitive solution-phase peptide synthesis procedures, this approach does not require time-consuming neutralisation reactions and reduces significantly the number of operation units that are necessary to obtain peptide intermediates. The efficiency of the new method is demonstrated by the rapid synthesis of short hydrophobic and hydrophilic peptides, the antimalarial cyclopeptide mahafacyclin B and a protected form of the hydrophilic pentapeptide Gly-Arg-Gly-Asp-Ser. Binding studies of the complex between 1-(3-Dimethylaminopropyl)-3-ethylurea hydrochloride (EDU.HCl) and triethylammonium trifluoroacetate are described and the potential application of EDU.HCl as an artificial carboxylate receptor to increase the acidity of trifluoroacetic acid is discussed.
Subjects/Keywords: 547.7; Peptide synthesis
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MLA ·
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Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Carnapete Alves Meneses, C. C. (2010). The development of a facile solution-phase method for the synthesis of peptides. (Doctoral Dissertation). University of Aberdeen. Retrieved from http://digitool.abdn.ac.uk:80/webclient/DeliveryManager?pid=166204 ; http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.540483
Chicago Manual of Style (16th Edition):
Carnapete Alves Meneses, Célia Clarisse. “The development of a facile solution-phase method for the synthesis of peptides.” 2010. Doctoral Dissertation, University of Aberdeen. Accessed September 19, 2019.
http://digitool.abdn.ac.uk:80/webclient/DeliveryManager?pid=166204 ; http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.540483.
MLA Handbook (7th Edition):
Carnapete Alves Meneses, Célia Clarisse. “The development of a facile solution-phase method for the synthesis of peptides.” 2010. Web. 19 Sep 2019.
Vancouver:
Carnapete Alves Meneses CC. The development of a facile solution-phase method for the synthesis of peptides. [Internet] [Doctoral dissertation]. University of Aberdeen; 2010. [cited 2019 Sep 19].
Available from: http://digitool.abdn.ac.uk:80/webclient/DeliveryManager?pid=166204 ; http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.540483.
Council of Science Editors:
Carnapete Alves Meneses CC. The development of a facile solution-phase method for the synthesis of peptides. [Doctoral Dissertation]. University of Aberdeen; 2010. Available from: http://digitool.abdn.ac.uk:80/webclient/DeliveryManager?pid=166204 ; http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.540483
15.
Erhardt, Nola Marlene.
The role of pituitary adenylate cyclase-activating polypeptide (PACAP) in cell cycle exit, differentiation and apoptosis during early chick brain development.
Degree: Department of Biology, 2017, University of Victoria
URL: http://hdl.handle.net/1828/7910
► Regulated survival, proliferation and differentiation of cells in the nervous system is crucial for development. Much of regulation is controlled by hormones. There is abundant…
(more)
▼ Regulated survival, proliferation and differentiation of cells in the nervous
system is crucial for development. Much of regulation is controlled by hormones.
There is abundant evidence that a member of the glucagon superfamily, pituitary
adenylate cyclase-activating polypeptide (PACAP), is important in this process. PACAP
functions have been described in the peripheral and central nervous systems of many
species. Although the primary function of PACAP is not known, its high conservation
and presence in all species examined to date suggest it is vital to normal development.
My thesis objective was to determine the response of early CNS neuroblasts to
PACAP, in conjunction with another glucagon superfamily member, growth hormone releasing hormone (GHRH). GHRH is best known for causing release of growth hormone from the pituitary, but it also has functions in nervous system development.
Because PACAP and GHRH are encoded on the same gene in non-mammalian vertebrates, it is possible that they have similar or coordinated functions. PACAP affects development by altering levels of proliferation and differentiation and decreasing
apoptosis. For these reasons, I focused my research in these areas.
Using neuroblast-enriched cultures from embryonic day 3.5 chick, my first goal was to show that PACAP and GHRH affected these cells. Radioimmunoassays for cAMP revealed that all but one form of PACAP, and only one form of GHRH, caused an
increase in cAMP relative to controls. As to the former, comparison of differing PACAP structures suggested that conservation at the amino terminus was important in binding the hormone to the receptor. The fact that PACAP, but not GHRH, increased
cAMP, indicated that evolution of PACAP and GHRH had altered their functions. Chick neuroblasts were also shown to produce PACAP and its primary receptor, suggesting an autocrine/paracrine role for PACAP.
My next goal was to examine the nature of the downstream effects of increased cAMP. To study cell cycle, I developed a protocol using proliferating cell nuclear antigen (PCNA) and propidium iodide (PI), in fixed cell populations. PCNA is present
in low amounts in non-cycling cells, but rises sharply in actively proliferating cells. The PI helped delineate cell cycle compartments, because in permeabilized cells it binds to and quantifies DNA. Changes in G0, G1, S and G2/M were recorded using flow cytometry. Because the cells were producing PACAP and most were cycling, rather than add more PACAP I chose to block the PACAP receptor. This caused cell cycle exit. I also blocked the cell cycle at two points, and showed that exogenous PACAP could release some cells from the block, and return them to cycling. PACAP affected apoptosis also, but because the protocol was not designed to measure this, I adopted another protocol using flow cytometry. With live cells, and fluorescein diacetate, which is retained and fluoresces in healthy cells, and PI, which enters only cells with damaged membranes, I used the characteristic of apoptotic cells to die…
Advisors/Committee Members: Sherwood, Nancy (supervisor).
Subjects/Keywords: Peptide hormones; Hormones
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APA ·
Chicago ·
MLA ·
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CSE |
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APA (6th Edition):
Erhardt, N. M. (2017). The role of pituitary adenylate cyclase-activating polypeptide (PACAP) in cell cycle exit, differentiation and apoptosis during early chick brain development. (Thesis). University of Victoria. Retrieved from http://hdl.handle.net/1828/7910
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Erhardt, Nola Marlene. “The role of pituitary adenylate cyclase-activating polypeptide (PACAP) in cell cycle exit, differentiation and apoptosis during early chick brain development.” 2017. Thesis, University of Victoria. Accessed September 19, 2019.
http://hdl.handle.net/1828/7910.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Erhardt, Nola Marlene. “The role of pituitary adenylate cyclase-activating polypeptide (PACAP) in cell cycle exit, differentiation and apoptosis during early chick brain development.” 2017. Web. 19 Sep 2019.
Vancouver:
Erhardt NM. The role of pituitary adenylate cyclase-activating polypeptide (PACAP) in cell cycle exit, differentiation and apoptosis during early chick brain development. [Internet] [Thesis]. University of Victoria; 2017. [cited 2019 Sep 19].
Available from: http://hdl.handle.net/1828/7910.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Erhardt NM. The role of pituitary adenylate cyclase-activating polypeptide (PACAP) in cell cycle exit, differentiation and apoptosis during early chick brain development. [Thesis]. University of Victoria; 2017. Available from: http://hdl.handle.net/1828/7910
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
University of Manchester
16.
Wu, Ming-Cheng.
Bio-engineering of Antibiotic Enduracidin Biosynthetic
Pathways and PreQ1 Riboswitch.
Degree: 2011, University of Manchester
URL: http://www.manchester.ac.uk/escholar/uk-ac-man-scw:138266
► Non-ribosomally synthesised natural products derived mainly from bacteria and fungi act as important therapeutic agents. Due to their complex structures it is difficult to chemically…
(more)
▼ Non-ribosomally synthesised natural products
derived mainly from bacteria and fungi act as important therapeutic
agents. Due to their complex structures it is difficult to
chemically synthesise such compounds, therefore the engineering of
biosynthetic and biocatalytic pathways that are vital for their
production are the aims of this thesis. The first project involved
the study of the biosynthesis of 3-O-methyl aspartic acid (OmAsp)
in the antibiotic A54145. We demonstrated that LptL functions as an
asparagine hydroxylase. We also predicted that LptJ and LptK are
involved in the biosynthesis of OmAsp, although we did not find
evidence of this non-standard amino acid in the antibiotic CDA when
we over-expressed both proteins in Streptomyces coelicolor. The
second project involved the study of the chlorination and
mannosylation of hydroxyphenyl glycine (Hpg) residues in
ramoplanin. We over-expressed the putative chlorinase and mannosyl
transferase separately in the enduracidin-producer, in which the
production of enduracidin has been characterised. We then found
that trichlorinated and mannosylated enduracidin analogues were
produced.The final project involved the re-engineering of a preQ1
riboswitch. We successfully created ten preQ1 riboswitch mutants
fused to the reporter gene lacZ in Bacillus subtilis, all of which
showed different levels of β-galactosidase activity. Subsequently,
we found that the preQ1 C17U mutant riboswitch can respond
specifically to the synthetic ligands
5-(aminomethyl)furo[2,3-d]pyrimidine-2,4-diamine (D6) and
2,4-diamino-7H-pyrrolo[2,3-d]pyrimidine-5-carbonitrile (D9) to
control gene expression in a dose dependent manner. The results
described here show the successful production of variant
antibiotics by bio-engineering and the use of an engineered preQ1
riboswitch as a tool for regulating gene
expression.
Advisors/Committee Members: Micklefield, Jason.
Subjects/Keywords: non-ribosomal peptide
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APA ·
Chicago ·
MLA ·
Vancouver ·
CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Wu, M. (2011). Bio-engineering of Antibiotic Enduracidin Biosynthetic
Pathways and PreQ1 Riboswitch. (Doctoral Dissertation). University of Manchester. Retrieved from http://www.manchester.ac.uk/escholar/uk-ac-man-scw:138266
Chicago Manual of Style (16th Edition):
Wu, Ming-Cheng. “Bio-engineering of Antibiotic Enduracidin Biosynthetic
Pathways and PreQ1 Riboswitch.” 2011. Doctoral Dissertation, University of Manchester. Accessed September 19, 2019.
http://www.manchester.ac.uk/escholar/uk-ac-man-scw:138266.
MLA Handbook (7th Edition):
Wu, Ming-Cheng. “Bio-engineering of Antibiotic Enduracidin Biosynthetic
Pathways and PreQ1 Riboswitch.” 2011. Web. 19 Sep 2019.
Vancouver:
Wu M. Bio-engineering of Antibiotic Enduracidin Biosynthetic
Pathways and PreQ1 Riboswitch. [Internet] [Doctoral dissertation]. University of Manchester; 2011. [cited 2019 Sep 19].
Available from: http://www.manchester.ac.uk/escholar/uk-ac-man-scw:138266.
Council of Science Editors:
Wu M. Bio-engineering of Antibiotic Enduracidin Biosynthetic
Pathways and PreQ1 Riboswitch. [Doctoral Dissertation]. University of Manchester; 2011. Available from: http://www.manchester.ac.uk/escholar/uk-ac-man-scw:138266
University of Manchester
17.
Szkolar, Laura.
The Development of Functional Peptide Scaffolds for Cell
Culture.
Degree: 2016, University of Manchester
URL: http://www.manchester.ac.uk/escholar/uk-ac-man-scw:298798
► Peptides and peptide derivatives have shown great scope as biomaterials and for biomedicaltherapy application. It has been demonstrated that classes of these peptides can form…
(more)
▼ Peptides and
peptide derivatives have shown great
scope as biomaterials and for biomedicaltherapy application. It has
been demonstrated that classes of these peptides can form fibrillar
hydrogels making them a good candidate for ECM mimics. In
particular, the ionic complementary peptides, composed of
alternating hydrophobic and hydrophilic amino acidshave been
reported as successful cell scaffolds. The simple structure of such
ionic complementary peptides is generally seen to spontaneously
self-assemble into β-sheet richfibrils in the presence of water.
The highly aqueous environment, along with the inter meshing of
fibres, results in an architecture akin to the natural ECM of the
body, making
peptide hydrogels highly suitable as cell culture
scaffolds. The structure of such hydrogels, usually comprising 8-32
amino acids, has been widely reported as easily modifiable, thus,
allowing for control of the final material properties.This study
explores the potential use of a range of ionic-complementary
peptides for the culture of primary bovine chondrocytes.
Modifications and additions to
peptide sequence, such as charge and
amino acid substitution, were investigated. In all studies only 1
design parameter (sequence, charge etc.) was varied, to allow for
better understanding of the effect of materials properties upon
cell response.The encapsulation of primary bovine chondrocytes was
undertaken, with the aim of providing a suitable cell scaffold
capable of maintaining chondrocyte viability and function in vitro.
Despite in vivo work being beyond the scope of this thesis, the
properties of the hydrogel scaffold were designed with final aim of
being suitable for use with matrix associated autologous
chondrocyte implantation (MACI) in clinical
therapy.
Advisors/Committee Members: GOUGH, JULIE JE, Gough, Julie, Saiani, Alberto.
Subjects/Keywords: peptide; chondrocyte; hydrogel
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APA ·
Chicago ·
MLA ·
Vancouver ·
CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Szkolar, L. (2016). The Development of Functional Peptide Scaffolds for Cell
Culture. (Doctoral Dissertation). University of Manchester. Retrieved from http://www.manchester.ac.uk/escholar/uk-ac-man-scw:298798
Chicago Manual of Style (16th Edition):
Szkolar, Laura. “The Development of Functional Peptide Scaffolds for Cell
Culture.” 2016. Doctoral Dissertation, University of Manchester. Accessed September 19, 2019.
http://www.manchester.ac.uk/escholar/uk-ac-man-scw:298798.
MLA Handbook (7th Edition):
Szkolar, Laura. “The Development of Functional Peptide Scaffolds for Cell
Culture.” 2016. Web. 19 Sep 2019.
Vancouver:
Szkolar L. The Development of Functional Peptide Scaffolds for Cell
Culture. [Internet] [Doctoral dissertation]. University of Manchester; 2016. [cited 2019 Sep 19].
Available from: http://www.manchester.ac.uk/escholar/uk-ac-man-scw:298798.
Council of Science Editors:
Szkolar L. The Development of Functional Peptide Scaffolds for Cell
Culture. [Doctoral Dissertation]. University of Manchester; 2016. Available from: http://www.manchester.ac.uk/escholar/uk-ac-man-scw:298798
Tulane University
18.
Starr, Charles.
Development of Novel Antimicrobial Peptides with Improved Hemocompatibility Through Combinatorial Library Screening and Rational Sequence Engineering.
Degree: 2017, Tulane University
URL: https://digitallibrary.tulane.edu/islandora/object/tulane:77175
► Development of antimicrobial peptides (AMPs) as next generation clinical antibiotics has been a pursuit of the scientific community for several decades. AMPs are attractive drug…
(more)
▼ Development of antimicrobial peptides (AMPs) as next generation clinical antibiotics has been a pursuit of the scientific community for several decades. AMPs are attractive drug candidates because of their potent antibacterial activity and a low propensity for eliciting antibiotic resistant bacterial phenotypes. However, despite substantial efforts and myriad development approaches, AMPs have yet to make inroads in the clinic due to toxicity concerns and activity loss in vivo. We hypothesized that eukaryotic cytotoxicity and antibacterial activity loss are intricately related in that peptide-induced tissue or host cell damage corresponds to depletion of free peptide available to target bacterial cells. Using human red blood cells (RBCs) as a model eukaryotic cell, we demonstrate that a cross-section of AMPs lose appreciable antibacterial activity when preincubated with concentrated eukaryotic cells (1x109 red blood cells/mL) and that this behavior can be explained by plasma membrane binding. To approach this problem in a unique manner, we synthesized a combinatorial peptide library based on the potent AMP, ARVA, and screened the library for activity in the presence of concentrated RBCs. We isolated nine unique, but similar sequences from the screen. During the screening program, we discovered that RBC-peptide interactions lead to peptide degradation through the release of cytosolic proteases. We used this knowledge to design a consensus sequence based on the nine peptides isolated from the library screen and synthesized it using only D-isomer amino acids. The novel peptide displays excellent antimicrobial activity against several human pathogens in the presence and absence of concentrated RBCs, has reduced toxicity towards eukaryotic cells, and is not susceptible to cleavage by cellular proteases. We attempted to use this peptide, D-NOGCON, to combat P. aeruginosa in a mouse model of acute pneumonia, but were unable to ameliorate the negative outcomes associated with infection. We ultimately suggest alternative models of bacterial infection in which the peptide may be more effective and future approaches to further refining the sequence of D-NOGCON.
1
Charles Starr
Advisors/Committee Members: Wimley, William (Thesis advisor), School of Medicine Biomedical Sciences Graduate Program (Degree granting institution), NULL (Degree granting institution).
Subjects/Keywords: biochemistry; antimicrobial; peptide
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APA ·
Chicago ·
MLA ·
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CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Starr, C. (2017). Development of Novel Antimicrobial Peptides with Improved Hemocompatibility Through Combinatorial Library Screening and Rational Sequence Engineering. (Thesis). Tulane University. Retrieved from https://digitallibrary.tulane.edu/islandora/object/tulane:77175
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Starr, Charles. “Development of Novel Antimicrobial Peptides with Improved Hemocompatibility Through Combinatorial Library Screening and Rational Sequence Engineering.” 2017. Thesis, Tulane University. Accessed September 19, 2019.
https://digitallibrary.tulane.edu/islandora/object/tulane:77175.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Starr, Charles. “Development of Novel Antimicrobial Peptides with Improved Hemocompatibility Through Combinatorial Library Screening and Rational Sequence Engineering.” 2017. Web. 19 Sep 2019.
Vancouver:
Starr C. Development of Novel Antimicrobial Peptides with Improved Hemocompatibility Through Combinatorial Library Screening and Rational Sequence Engineering. [Internet] [Thesis]. Tulane University; 2017. [cited 2019 Sep 19].
Available from: https://digitallibrary.tulane.edu/islandora/object/tulane:77175.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Starr C. Development of Novel Antimicrobial Peptides with Improved Hemocompatibility Through Combinatorial Library Screening and Rational Sequence Engineering. [Thesis]. Tulane University; 2017. Available from: https://digitallibrary.tulane.edu/islandora/object/tulane:77175
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
University of New South Wales
19.
Wang, Zhiyong.
RGD peptide derivatives as tools for tumour imaging and therapy.
Degree: Chemical Sciences & Engineering, 2013, University of New South Wales
URL: http://handle.unsw.edu.au/1959.4/52850
;
https://unsworks.unsw.edu.au/fapi/datastream/unsworks:11523/SOURCE01?view=true
► The first project is a tumour imaging method based on iodinated micelle which can be applied as contrast agent for CT. the obtained micelle solution…
(more)
▼ The first project is a tumour imaging method based on iodinated micelle which can be applied as contrast agent for CT. the obtained micelle solution was tested by x-ray instrument and shows acceptable contrast ability in comparison with commercial samples. The second project is to develop a novel tumour therapy aimed at blocking angiogenesis process which has been proved to be crucial for growth of tumour cells. RGD as common cell recognition topic is employed in the project to synthesize fluorinated tetra-
peptide abridged by fluorinated GABA amino aicds. Fluorinated amino acid is synthesized according to previous literature, efforts have also been made to optimise reaction conditions. A rationale is proposed for vital second fluorination reactions during condition optimization. We observed a cyclization efficiency difference owing to conformational variation of fluorinated amino acids. Fluorinated cyclic RGD peptides is synthesized and after deprotection peptides will be tested on angiogenesis assay and integrin adhesion assay in collaboration with GSK England and CCIA in Sydney
Advisors/Committee Members: Hunter, Luke, Chemistry, Faculty of Science, UNSW.
Subjects/Keywords: Peptide; RGD; Fluorine
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APA ·
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MLA ·
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Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Wang, Z. (2013). RGD peptide derivatives as tools for tumour imaging and therapy. (Masters Thesis). University of New South Wales. Retrieved from http://handle.unsw.edu.au/1959.4/52850 ; https://unsworks.unsw.edu.au/fapi/datastream/unsworks:11523/SOURCE01?view=true
Chicago Manual of Style (16th Edition):
Wang, Zhiyong. “RGD peptide derivatives as tools for tumour imaging and therapy.” 2013. Masters Thesis, University of New South Wales. Accessed September 19, 2019.
http://handle.unsw.edu.au/1959.4/52850 ; https://unsworks.unsw.edu.au/fapi/datastream/unsworks:11523/SOURCE01?view=true.
MLA Handbook (7th Edition):
Wang, Zhiyong. “RGD peptide derivatives as tools for tumour imaging and therapy.” 2013. Web. 19 Sep 2019.
Vancouver:
Wang Z. RGD peptide derivatives as tools for tumour imaging and therapy. [Internet] [Masters thesis]. University of New South Wales; 2013. [cited 2019 Sep 19].
Available from: http://handle.unsw.edu.au/1959.4/52850 ; https://unsworks.unsw.edu.au/fapi/datastream/unsworks:11523/SOURCE01?view=true.
Council of Science Editors:
Wang Z. RGD peptide derivatives as tools for tumour imaging and therapy. [Masters Thesis]. University of New South Wales; 2013. Available from: http://handle.unsw.edu.au/1959.4/52850 ; https://unsworks.unsw.edu.au/fapi/datastream/unsworks:11523/SOURCE01?view=true
University of Edinburgh
20.
Svensen, Nina.
Cellular analysis and PNA encoded libraries.
Degree: PhD, 2011, University of Edinburgh
URL: http://hdl.handle.net/1842/9605
► A peptide nucleic acid (PNA) encoded 1296 member peptide library was synthesised and incubated with a variety of cell types. Library members entering cells were…
(more)
▼ A peptide nucleic acid (PNA) encoded 1296 member peptide library was synthesised and incubated with a variety of cell types. Library members entering cells were extracted, hybridised onto DNA microarrays and the peptide identity was determined via deconvolution. Global consensus analysis highlighted the tetrapepide, Glu-Llp- Glu-Glu (Llp is 6-hexamine-N-aminoacetic acid), a surprise in view of the basic residues typically observed in cell penetrating peptides. When evaluated, Glu-Llp- Glu-Glu revealed cellular uptake comparable to a known basic peptide (tetraLlp). In depth delineation via clustering analysis allowed assessment of differential cellular uptake, with the identified peptides showing clear cellular specificity. This was verified by peptide synthesis and cellular uptake analysis by flow-cytometry, and in all cases an endocytic uptake mechanism was confirmed. This approach establishes a strategy for the identification of short peptides as
tools for selective delivery into specific cell types. The incubation of a 10,000 member PNA-encoded peptide library with D54 and HEK293T transfected with CCR6 cells followed by microarray analysis allowed detailed information on the interaction between peptide-ligands and cell surface receptors to be extracted. This allowed the identification of new ligands for integrins and G-protein coupled receptors and offers a novel approach to ligand discovery allowing the comparative analysis of different cell types for the identification of differences in surface-receptor ligands and/or receptor expression between various cell types. In addition, this work included the development of a novel method for the indirect amplification of a PNA library by amplification of a complementary DNA library hybridised to the PNA. The generation of 10,000 defined pieces of DNA would have a myriad of applications, not least in the area of defined or directed sequencing and synthetic biology, but also in
applications associated with encoding and tagging. By this approach DNA microarrays were used to allow the linear amplification of immobilised DNA sequences on an array followed by PCR amplification. Arrays of increasing sophistication (1; 10; 3875; 10,000 defined oligonucleotides) were used to validate the process, with sequences verified by selective hybridisation to a complementary DNA microarray with DNA sequencing demonstrating error rates of ca ≈ 0.2%. This technique offers an economical and efficient way of producing hundreds to thousands of specific DNA primers, while the DNA-arrays can be used as “factories” allowing specific DNA oligonucleotide pools to be generated with or without masking. This study also demonstrated a significant variance observed between the sequence frequencies found via Solexa sequencing compared to microarray analysis.
Subjects/Keywords: 572.8; peptide nucleic acid; peptide library; endocytic uptake mechanism; peptide-ligands
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APA ·
Chicago ·
MLA ·
Vancouver ·
CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Svensen, N. (2011). Cellular analysis and PNA encoded libraries. (Doctoral Dissertation). University of Edinburgh. Retrieved from http://hdl.handle.net/1842/9605
Chicago Manual of Style (16th Edition):
Svensen, Nina. “Cellular analysis and PNA encoded libraries.” 2011. Doctoral Dissertation, University of Edinburgh. Accessed September 19, 2019.
http://hdl.handle.net/1842/9605.
MLA Handbook (7th Edition):
Svensen, Nina. “Cellular analysis and PNA encoded libraries.” 2011. Web. 19 Sep 2019.
Vancouver:
Svensen N. Cellular analysis and PNA encoded libraries. [Internet] [Doctoral dissertation]. University of Edinburgh; 2011. [cited 2019 Sep 19].
Available from: http://hdl.handle.net/1842/9605.
Council of Science Editors:
Svensen N. Cellular analysis and PNA encoded libraries. [Doctoral Dissertation]. University of Edinburgh; 2011. Available from: http://hdl.handle.net/1842/9605
University of Illinois – Chicago
21.
Jaishankar, Dinesh.
Engineering a Higher Efficacy Anti-Heparan Sulfate Peptide for an Entry-Based Antiviral Therapy.
Degree: 2017, University of Illinois – Chicago
URL: http://hdl.handle.net/10027/22031
► Primary and recurring herpes simplex virus-1 (HSV-1) infections can cause different pathologies in the eye and in severe cases it can result in permanent blindness.…
(more)
▼ Primary and recurring herpes simplex virus-1 (HSV-1) infections can cause different pathologies in the eye and in severe cases it can result in permanent blindness. The current antiviral treatments for HSV-1 belong to a class of nucleoside analogs that inhibit viral DNA polymerase and block viral replication. Since they all target the same step in viral lifecycle, emergence of drug resistance is on the rise. Topical antivirals get eliminated through absorbance from ocular tissues or through the nasolacrimal duct resulting in lower bioavailability and frequent applications to reduce symptoms. These challenges need to be addressed by developing newer treatment options that target different stages in the viral lifecycle and by utilizing innovative technologies to enhance the efficacy and residence time of a drug on the cornea. Previous work from our lab discovered a unique arginine-rich
peptide called G2. The
peptide was specifically isolated against heparan sulfate (HS). HSV-1 used HS for attaching to cells and initiating entry. The G2
peptide binds to HS and inhibits viral entry and subsequent infection. Although peptides are fast growing as potential therapeutic molecules due to their high specificity, they are susceptible to protease degradation resulting in low activity and availability. The goal of this study is to increase the efficacy and/or stability of the G2
peptide that can either be used as a standalone preventive management or as a therapeutic in combination with existing antiviral treatments in controlling ocular herpes infection. Using
peptide conjugation and
peptide structure modification strategies and contact lenses as a drug delivery system, this study shows that the modified peptides are stable and significantly block viral entry and subsequent infection. The modified peptides are the first of its kind and may represent a new class of antiviral compounds. Additionally, other microorganisms such as fungi and bacteria also use HS for their pathogenesis. Thus, these efficacious forms of G2 may represent a broad-spectrum drug against these microbes.
Advisors/Committee Members: Shukla, Deepak (advisor).
Subjects/Keywords: peptide engineering; herpes simplex virus; contact lens; d-peptide; peptide therapeutics; cornea
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APA ·
Chicago ·
MLA ·
Vancouver ·
CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Jaishankar, D. (2017). Engineering a Higher Efficacy Anti-Heparan Sulfate Peptide for an Entry-Based Antiviral Therapy. (Thesis). University of Illinois – Chicago. Retrieved from http://hdl.handle.net/10027/22031
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Jaishankar, Dinesh. “Engineering a Higher Efficacy Anti-Heparan Sulfate Peptide for an Entry-Based Antiviral Therapy.” 2017. Thesis, University of Illinois – Chicago. Accessed September 19, 2019.
http://hdl.handle.net/10027/22031.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Jaishankar, Dinesh. “Engineering a Higher Efficacy Anti-Heparan Sulfate Peptide for an Entry-Based Antiviral Therapy.” 2017. Web. 19 Sep 2019.
Vancouver:
Jaishankar D. Engineering a Higher Efficacy Anti-Heparan Sulfate Peptide for an Entry-Based Antiviral Therapy. [Internet] [Thesis]. University of Illinois – Chicago; 2017. [cited 2019 Sep 19].
Available from: http://hdl.handle.net/10027/22031.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Jaishankar D. Engineering a Higher Efficacy Anti-Heparan Sulfate Peptide for an Entry-Based Antiviral Therapy. [Thesis]. University of Illinois – Chicago; 2017. Available from: http://hdl.handle.net/10027/22031
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
University of Manchester
22.
Wu, Ming-Cheng.
Bio-engineering of antibiotic enduracidin biosynthetic pathways and PreQ1 riboswitch.
Degree: PhD, 2011, University of Manchester
URL: https://www.research.manchester.ac.uk/portal/en/theses/bioengineering-of-antibiotic-enduracidin-biosynthetic-pathways-and-preq1-riboswitch(5eb04bf7-f20b-49d0-96f6-cd06f92676d1).html
;
http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.701088
► Non-ribosomally synthesised natural products derived mainly from bacteria and fungi act as important therapeutic agents. Due to their complex structures it is difficult to chemically…
(more)
▼ Non-ribosomally synthesised natural products derived mainly from bacteria and fungi act as important therapeutic agents. Due to their complex structures it is difficult to chemically synthesise such compounds, therefore the engineering of biosynthetic and biocatalytic pathways that are vital for their production are the aims of this thesis. The first project involved the study of the biosynthesis of 3-O-methyl aspartic acid (OmAsp) in the antibiotic A54145. We demonstrated that LptL functions as an asparagine hydroxylase. We also predicted that LptJ and LptK are involved in the biosynthesis of OmAsp, although we did not find evidence of this non-standard amino acid in the antibiotic CDA when we over-expressed both proteins in Streptomyces coelicolor. The second project involved the study of the chlorination and mannosylation of hydroxyphenyl glycine (Hpg) residues in ramoplanin. We over-expressed the putative chlorinase and mannosyl transferase separately in the enduracidin-producer, in which the production of enduracidin has been characterised. We then found that trichlorinated and mannosylated enduracidin analogues were produced. The final project involved the re-engineering of a preQ1 riboswitch. We successfully created ten preQ1 riboswitch mutants fused to the reporter gene lacZ in Bacillus subtilis, all of which showed different levels of β-galactosidase activity. Subsequently, we found that the preQ1 C17U mutant riboswitch can respond specifically to the synthetic ligands 5-(aminomethyl)furo[2,3-d]pyrimidine-2,4-diamine (D6) and 2,4-diamino-7H-pyrrolo[2,3-d]pyrimidine-5-carbonitrile (D9) to control gene expression in a dose dependent manner. The results described here show the successful production of variant antibiotics by bio-engineering and the use of an engineered preQ1 riboswitch as a tool for regulating gene expression.
Subjects/Keywords: 572; non-ribosomal peptide
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APA (6th Edition):
Wu, M. (2011). Bio-engineering of antibiotic enduracidin biosynthetic pathways and PreQ1 riboswitch. (Doctoral Dissertation). University of Manchester. Retrieved from https://www.research.manchester.ac.uk/portal/en/theses/bioengineering-of-antibiotic-enduracidin-biosynthetic-pathways-and-preq1-riboswitch(5eb04bf7-f20b-49d0-96f6-cd06f92676d1).html ; http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.701088
Chicago Manual of Style (16th Edition):
Wu, Ming-Cheng. “Bio-engineering of antibiotic enduracidin biosynthetic pathways and PreQ1 riboswitch.” 2011. Doctoral Dissertation, University of Manchester. Accessed September 19, 2019.
https://www.research.manchester.ac.uk/portal/en/theses/bioengineering-of-antibiotic-enduracidin-biosynthetic-pathways-and-preq1-riboswitch(5eb04bf7-f20b-49d0-96f6-cd06f92676d1).html ; http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.701088.
MLA Handbook (7th Edition):
Wu, Ming-Cheng. “Bio-engineering of antibiotic enduracidin biosynthetic pathways and PreQ1 riboswitch.” 2011. Web. 19 Sep 2019.
Vancouver:
Wu M. Bio-engineering of antibiotic enduracidin biosynthetic pathways and PreQ1 riboswitch. [Internet] [Doctoral dissertation]. University of Manchester; 2011. [cited 2019 Sep 19].
Available from: https://www.research.manchester.ac.uk/portal/en/theses/bioengineering-of-antibiotic-enduracidin-biosynthetic-pathways-and-preq1-riboswitch(5eb04bf7-f20b-49d0-96f6-cd06f92676d1).html ; http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.701088.
Council of Science Editors:
Wu M. Bio-engineering of antibiotic enduracidin biosynthetic pathways and PreQ1 riboswitch. [Doctoral Dissertation]. University of Manchester; 2011. Available from: https://www.research.manchester.ac.uk/portal/en/theses/bioengineering-of-antibiotic-enduracidin-biosynthetic-pathways-and-preq1-riboswitch(5eb04bf7-f20b-49d0-96f6-cd06f92676d1).html ; http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.701088
University of Alberta
23.
Binazadeh, Mojtaba.
Effect of Secondary Structure on Surface Adsorption of
Peptides.
Degree: PhD, Department of Chemical and Materials
Engineering, 2013, University of Alberta
URL: https://era.library.ualberta.ca/files/jh343v004
► Protein adsorption at the biomaterial-tissue interface has several detrimental consequences which undermines the widespread application of engineered materials. Herein, it was asked what role protein…
(more)
▼ Protein adsorption at the biomaterial-tissue interface
has several detrimental consequences which undermines the
widespread application of engineered materials. Herein, it was
asked what role protein secondary structures play in the adsorption
of peptides, as well as how these structures affect the
physicochemical properties of the final adsorbed layer on bare gold
(Au) and poly (ethylene glycol) (PEG) modified Au surfaces. To this
end, α-helices and -sheets were induced in poly-L-Lysine (PLL) and
persistence of these structures in solution and adsorbed state was
confirmed by circular dichroism (CD). PLL adsorption to Au surfaces
was monitored using quartz crystal microbalance with dissipation
(QCM-D). PLL adsorption on bare Au resulted in higher initial
adsorption rates for α-helices as compared to β-sheets but the
final adsorbed amount of β-sheets was higher than α-helices
regardless of solution salt concentration. Viscosities for films
formed from α-helices were ~2x that of β-sheets films, regardless
of solution ionic strength. β-sheets have higher zeta potential as
compared to α-helices. The interaction energy between PLL and Au
surface was found to be driven by electrostatic and van der Waals
forces. Presence of PEG grafted brush layers on the Au surface
drastically reduced the adsorbed amount of different PLL structures
and PLL layers adsorbed on PEG coated surfaces had similar layer
viscosities. To further understand PLL adsorption mechanism and
adsorbed layer properties, the interacting forces between PLL-Au,
PLL-PLL, PEG-mica, and PEG-PLL surfaces were studied by surface
forces apparatus (SFA). SFA results revealed that the adhesion
energy of β-sheet vs. Au and β-sheet vs. β-sheet was considerably
more than α-helix vs. Au and α-helix vs. α-helix systems
respectively. The substrate surface adhesion energy of β-sheet was
more dependent on the solution salt concentration as compared to
α-helix due to the higher electrostatic interactions of β-sheet PLL
film with Au (higher zeta potential value of β-sheet PLL). It was
found that presence of PEG grafted layer eliminated the PLL
secondary structure effect on adsorption due to the purely
repulsive force that governed the PEG vs. PLL
interaction.
Subjects/Keywords: Peptide; Surface Adsorption; Secondary Structure
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APA ·
Chicago ·
MLA ·
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CSE |
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Manager
APA (6th Edition):
Binazadeh, M. (2013). Effect of Secondary Structure on Surface Adsorption of
Peptides. (Doctoral Dissertation). University of Alberta. Retrieved from https://era.library.ualberta.ca/files/jh343v004
Chicago Manual of Style (16th Edition):
Binazadeh, Mojtaba. “Effect of Secondary Structure on Surface Adsorption of
Peptides.” 2013. Doctoral Dissertation, University of Alberta. Accessed September 19, 2019.
https://era.library.ualberta.ca/files/jh343v004.
MLA Handbook (7th Edition):
Binazadeh, Mojtaba. “Effect of Secondary Structure on Surface Adsorption of
Peptides.” 2013. Web. 19 Sep 2019.
Vancouver:
Binazadeh M. Effect of Secondary Structure on Surface Adsorption of
Peptides. [Internet] [Doctoral dissertation]. University of Alberta; 2013. [cited 2019 Sep 19].
Available from: https://era.library.ualberta.ca/files/jh343v004.
Council of Science Editors:
Binazadeh M. Effect of Secondary Structure on Surface Adsorption of
Peptides. [Doctoral Dissertation]. University of Alberta; 2013. Available from: https://era.library.ualberta.ca/files/jh343v004
University of Alberta
24.
Shahin, Mostafa H.
Development of polymer and lipid based nano-delivery systems
for targeted cancer chemotherapy.
Degree: PhD, Faculty of Pharmacy and Pharmaceutical
Sciences, 2012, University of Alberta
URL: https://era.library.ualberta.ca/files/k0698856b
► Conventional chemotherapy agents can kill tumor cells and inhibit tumor growth, but they produce severe side effects on normal cells at the same time. To…
(more)
▼ Conventional chemotherapy agents can kill tumor cells
and inhibit tumor growth, but they produce severe side effects on
normal cells at the same time. To shift the balance towards tumoral
effects, it would be desirable to direct the anti-cancer drug
towards tumor while restricting drug access to normal tissues. The
main objective of this thesis was to develop tumor targeted drug
delivery system that can take on this task and as a result, improve
the specificity and anticancer activity of the incorporated
anticancer drugs towards tumor. To this end, lipid and block
copolymer based nano-delivery systems of two conventional
anti-cancer agents doxorubicin (DOX) and paclitaxel (PTX) are
developed, respectively, and modified on their surface with a 12mer
breast tumor interacting peptide, namely p160, or its engineered
derivatives developed in our research team. The effect of peptide
decoration on the specific interaction as well as anti-cancer
activity of developed nano-formulations against human breast tumor
cells over normal epithelial breast cells or endothelial cells was
characterized, in vitro. In this study, micelles of poly(ethylene
oxide)-b-poly(-caprolactone) were prepared and modified with
either c(RGDfK) or p160 and loaded with paclitaxel (PTX). Peptide
decoration enhanced the selective cytotoxicity of encapsulated PTX
against cancer cells over normal cells. The extent of this increase
in cancer cell specificity for encapsulated PTX was more for
p160-modified micelles. At the end, the anti-cancer activity of
liposomal formulations of DOX, having different density of an
engineered breast tumor targeting peptide, namely p-18-4, was
assessed in vitro for cellular uptake and selective cytotoxicity,
and in vivo using animal model of human breast tumor xenograft and
compared to that for liposomal formulations of DOX with no peptide
decoration. Liposomal DOX formulations bearing low p18-4 density
showed better in vitro selective cytotoxicity and in vivo
therapeutic efficacy. Our results points to the potential of p160
and its engineered derivatives as efficient ligands on lipid and
block copolymer based nanocarriers for active targeting of
anticancer agents to breast tumors. The results also show the
success of this strategy in enhancing the specific anti-cancer
effects of the incorporated drug against breast tumor
models.
Subjects/Keywords: peptide; liposomes; chemotherapy; polymeric micelle
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APA ·
Chicago ·
MLA ·
Vancouver ·
CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Shahin, M. H. (2012). Development of polymer and lipid based nano-delivery systems
for targeted cancer chemotherapy. (Doctoral Dissertation). University of Alberta. Retrieved from https://era.library.ualberta.ca/files/k0698856b
Chicago Manual of Style (16th Edition):
Shahin, Mostafa H. “Development of polymer and lipid based nano-delivery systems
for targeted cancer chemotherapy.” 2012. Doctoral Dissertation, University of Alberta. Accessed September 19, 2019.
https://era.library.ualberta.ca/files/k0698856b.
MLA Handbook (7th Edition):
Shahin, Mostafa H. “Development of polymer and lipid based nano-delivery systems
for targeted cancer chemotherapy.” 2012. Web. 19 Sep 2019.
Vancouver:
Shahin MH. Development of polymer and lipid based nano-delivery systems
for targeted cancer chemotherapy. [Internet] [Doctoral dissertation]. University of Alberta; 2012. [cited 2019 Sep 19].
Available from: https://era.library.ualberta.ca/files/k0698856b.
Council of Science Editors:
Shahin MH. Development of polymer and lipid based nano-delivery systems
for targeted cancer chemotherapy. [Doctoral Dissertation]. University of Alberta; 2012. Available from: https://era.library.ualberta.ca/files/k0698856b
University of Alberta
25.
Bodapati, Krishna Chaitanya.
Synthesis and SAR studies of antimicrobial peptide Leucocin
A.
Degree: MS, Faculty of Pharmacy and Pharmaceutical
Sciences, 2011, University of Alberta
URL: https://era.library.ualberta.ca/files/cc08hg66t
► In this study, we report the synthesis of a potent antimicrobial peptide Leucocin A (LeuA) using two solid phase peptide synthesis methods. One of the…
(more)
▼ In this study, we report the synthesis of a potent
antimicrobial peptide Leucocin A (LeuA) using two solid phase
peptide synthesis methods. One of the methods, native chemical
ligation, gave high yield (12.5%) of 37-residue LeuA and can be
utilized in the synthesis of LeuA to perform structure-activity
relationship (SAR) studies. Three analogues (1-3) of LeuA were
designed and synthesized to explore the SAR in the N-terminal
domain of LeuA. In the analogues, N-terminal -sheet residues
Cys9-Ser15 of the native peptide were replaced with shorter -turn
motifs. Such replacement abolished the antimicrobial activity in
all the analogues. Circular dichroism spectroscopy suggested that
only analogue 1 adopted similar folding as LeuA. Therefore, 1 was
able to competitively inhibit the activity of native LeuA. However,
analysis of the secondary structure of 1 using molecular dynamics
simulations suggested lack of -sheet formation in the N-terminal
region compared to LeuA emphasizing the role of proper folding and
sequence in the activity of class IIa bacteriocins.
Subjects/Keywords: antimicrobial peptide, leucocin a, bacteriocin
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APA ·
Chicago ·
MLA ·
Vancouver ·
CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Bodapati, K. C. (2011). Synthesis and SAR studies of antimicrobial peptide Leucocin
A. (Masters Thesis). University of Alberta. Retrieved from https://era.library.ualberta.ca/files/cc08hg66t
Chicago Manual of Style (16th Edition):
Bodapati, Krishna Chaitanya. “Synthesis and SAR studies of antimicrobial peptide Leucocin
A.” 2011. Masters Thesis, University of Alberta. Accessed September 19, 2019.
https://era.library.ualberta.ca/files/cc08hg66t.
MLA Handbook (7th Edition):
Bodapati, Krishna Chaitanya. “Synthesis and SAR studies of antimicrobial peptide Leucocin
A.” 2011. Web. 19 Sep 2019.
Vancouver:
Bodapati KC. Synthesis and SAR studies of antimicrobial peptide Leucocin
A. [Internet] [Masters thesis]. University of Alberta; 2011. [cited 2019 Sep 19].
Available from: https://era.library.ualberta.ca/files/cc08hg66t.
Council of Science Editors:
Bodapati KC. Synthesis and SAR studies of antimicrobial peptide Leucocin
A. [Masters Thesis]. University of Alberta; 2011. Available from: https://era.library.ualberta.ca/files/cc08hg66t
University of KwaZulu-Natal
26.
[No author].
Synthesis and aggregation dynamics of amylin.
Degree: Biochemistry, 2013, University of KwaZulu-Natal
URL: http://hdl.handle.net/10413/10076
► Amylin is a 37 amino acid long peptide that aggregates into toxic oligomers and fibrils. Since amylin is secreted by and also acts on pancreatic…
(more)
▼ Amylin is a 37 amino acid long
peptide that aggregates into toxic oligomers and fibrils. Since amylin is secreted by and also acts on pancreatic beta cells, type II diabetes is classified as an amyloidogenic disease. This study focuses on the development of a cost effective chemical synthetic strategy for amylin synthesis as previous studies relied on extremely expensive pseudoproline derivatives. Furthermore, commercially available amylin varies between
batches and also contains impurities that could generate anomalies and affect reproducibility of experiments. Secondly, chemically synthesized non-methylated and N-methylated derivatives of amylin were shown to inhibit toxicity of full length amylin. A fluorescentlylabeled chemically synthesized derivative of amylin was used to track cellular localization of amylin via confocal microscopy. Amylin aggregation kinetics was established using a surface plasmon resonance (SPR) biosensor. In addition, nanoparticle tracking analysis (NTA) was used as a novel technique to determine the size of oligomers over real time. This technology indicated that the size range of the toxic species of amylin is between 200-300 nm. Furthermore, it can be suggested that NTA could potentially be developed into a screening tool for inhibitors of amylin-mediated cytotoxicity.
Advisors/Committee Members: Govender, Patrick (advisor).
Subjects/Keywords: Amylin.;
Peptide hormones.;
Amyloid.;
Biochemistry.
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APA ·
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Export
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Manager
APA (6th Edition):
author], [. (2013). Synthesis and aggregation dynamics of amylin.
(Thesis). University of KwaZulu-Natal. Retrieved from http://hdl.handle.net/10413/10076
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
author], [No. “Synthesis and aggregation dynamics of amylin.
” 2013. Thesis, University of KwaZulu-Natal. Accessed September 19, 2019.
http://hdl.handle.net/10413/10076.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
author], [No. “Synthesis and aggregation dynamics of amylin.
” 2013. Web. 19 Sep 2019.
Vancouver:
author] [. Synthesis and aggregation dynamics of amylin.
[Internet] [Thesis]. University of KwaZulu-Natal; 2013. [cited 2019 Sep 19].
Available from: http://hdl.handle.net/10413/10076.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
author] [. Synthesis and aggregation dynamics of amylin.
[Thesis]. University of KwaZulu-Natal; 2013. Available from: http://hdl.handle.net/10413/10076
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
27.
Singh, Gurpreet.
Molecular simulation studies of
peptide aggregation in small finite sized systems and near
surfaces.
Degree: 2009, Technische Universität Dortmund
URL: http://hdl.handle.net/2003/26021
► Die Aggregation von Proteinen spielt in verschiedenen biologischen Prozessen eine Rolle und wird insbesondere mit vielen Krankheiten wie Alzheimer, Parkinson oder Type II Diabetes Mellitus…
(more)
▼ Die Aggregation von Proteinen
spielt in verschiedenen biologischen Prozessen eine Rolle und wird
insbesondere mit vielen Krankheiten wie Alzheimer, Parkinson oder
Type II Diabetes Mellitus in Verbindung gebracht. Es wurde gezeigt,
dass die Aggregation amyloidogener Proteine im Falle der Ausbildung
von amyloiden Fibrillen einem keimbasierten Wachstumsmechanismus
folgt. Die Aufspaltung einer wässrigen Proteinlösung in eine
gesättigte Proteinlösung und feste Fibrillen, die sich miteinander
im Gleichgewicht befinden, ist vergleichbar mit der Entmischung in
binären Systemen. Weil die Proteinaggregation in Zellen
zusätzlichen Einflüssen wie der Anwesenheit verschiedener
Oberflächen und Cosolventien und einer begrenzten Zellgröße
unterliegt, ist es wichtig, den Effekt dieser Einflüsse zu
verstehen. Einen Beitrag zur Aufklärung kann auch durch
Computersimulation von Modellpeptidfragmenten, die sich ähnlich
verhalten wie das Protein, aus dem sie entnommen wurden, geleistet
werden. Solche Studien haben den Vorteil, dass sie Informationen
auf molekularer Ebene liefern können. In dieser Arbeit wurden die
Auswirkungen einer endlichen Systemgröße, der Temperatur und der
Wechselwirkung mit Oberflächen auf die Aggregation untersucht. In
jeder Computersimulation, die sich mit Phasenübergängen
beschäftigt, müssen Oberflächeneffekte in die überlegungen auf
geeignete Weise einbezogen werden, bevor die Ergebnisse auf
makroskopische Systeme übertragen werden können. Aus diesem Grunde
wurde im Rahmen dieser Arbeit der Einfluss einer begrenzten
Systemgröße auf die Aggregation von Peptiden mit Hilfe der
molekulardynamischen Simulation wässriger Peptidlösungen unter
Verwendung verschiedener Konzentrationen und Systemgrößen
untersucht. In diesem Zusammenhang wurden drei verschiedene
Parameter verwendet, um den Aggregationsprozess zu quantifizieren
und den Aggregrationsgrad zu berechnen. Zunehmende
Aggregationsgrade ließen sich durch Erhöhung der
Peptidkonzentration bei gleich bleibender Systemgröße oder durch
Erhöhung der Systemgröße bei gleich bleibender Peptidkonzentration
erreichen. Die Abnahme des Aggregationsgrades mit der Abnahme der
Systemgröße wurde auf das Auftreten eines künstlichen Zustandes
zurückgeführt, in dem die weniger stark vertretene Phase, in diesem
Fall das Peptidaggregat, beeinflusst wird. Sobald sich die
Systemgröße an die Größe makroskopischer Systeme annähert,
verschwindet dieser künstliche Zustand schließlich. Folglich wird
eine Aggregation, die bei einer bestimmten Konzentration in einem
makroskopischen System auftritt, bei der gleichen Konzentration in
kleinen (mikroskopischen) Systemen unterdrückt. Aus den
durchgeführten Studien ergeben sich hauptsächlich zwei
Schlussfolgerungen: Zum einen hat die endliche Systemgröße einen
drastischen Einfluss auf die Aggregation von Peptiden und ist
verantwortlich für die Instabilität von Aggregaten, die aus nur
wenigen Peptiden bestehen. Wie bereits oben angesprochen, muss dies
bei der übertragung auf makroskopische Systeme berücksichtigt
werden. Zum anderen ist auch klar,…
Advisors/Committee Members: Winter, Roland.
Subjects/Keywords: Molecular simulation; Peptide
aggregation; 540
Record Details
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APA ·
Chicago ·
MLA ·
Vancouver ·
CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Singh, G. (2009). Molecular simulation studies of
peptide aggregation in small finite sized systems and near
surfaces. (Thesis). Technische Universität Dortmund. Retrieved from http://hdl.handle.net/2003/26021
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Singh, Gurpreet. “Molecular simulation studies of
peptide aggregation in small finite sized systems and near
surfaces.” 2009. Thesis, Technische Universität Dortmund. Accessed September 19, 2019.
http://hdl.handle.net/2003/26021.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Singh, Gurpreet. “Molecular simulation studies of
peptide aggregation in small finite sized systems and near
surfaces.” 2009. Web. 19 Sep 2019.
Vancouver:
Singh G. Molecular simulation studies of
peptide aggregation in small finite sized systems and near
surfaces. [Internet] [Thesis]. Technische Universität Dortmund; 2009. [cited 2019 Sep 19].
Available from: http://hdl.handle.net/2003/26021.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Singh G. Molecular simulation studies of
peptide aggregation in small finite sized systems and near
surfaces. [Thesis]. Technische Universität Dortmund; 2009. Available from: http://hdl.handle.net/2003/26021
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
NSYSU
28.
Huang, Huei-Jhen.
Influence of chemical conditions on human insulin fibril structure.
Degree: Master, Chemistry, 2013, NSYSU
URL: http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0713113-113753
► Many proteins can misfold to form non-native structures and induce aggregation. Previous studies had found that more than 20 kinds of proteins may cause specific…
(more)
▼ Many proteins can misfold to form non-native structures and induce aggregation. Previous studies had found that more than 20 kinds of proteins may cause specific diseases that under ineffective prevention and treatment, and greatly perplexed many patients and researchers. Either the amino acid sequences, or molecular size, or the folded three-dimensional conformation of these pathogenic proteins is quite different, all of them will lead to the formation of insoluble amyloid fibers after misfolding. Herein, we investigated the environmental changes by the alcohol contentãpH value and adding short peptides and dendrimers in solvent incubated with human insulin. We used circular dichroism (CD) spectroscopyãfourier transform infrared spectroscopy (FT-IR) and fluoresce spectroscopy to obtain the structural transition of the insulin, and gain the morphology information of fibril by atomic force microscopy (AFM). The results showed that the increasing percentage of alcohol or increasing carbon chain length of alcohols can cause a difference CD spectra. The surface morphology of insulin fibers by AFM imaging indicated that either increasing the percentage of alcohol or increasing alcohol carbon chain length will inhibit insulin fiber formation. This phenomenon was attributing to hydrophobic group expose to solution and solvent molecular may prevent nucleus to contact. And lowering the pH will undergo conformational change from α-helix to β-sheet form that causes the formation of amyloid plaques. This result may be attributed to Cl- that covered the surface charge of insulin fibril, then weaken the original electrostatic repulsion among insulin fibrils and result in their aggregation.
Advisors/Committee Members: Po-Chiao Lin (chair), Shu-chen Hsieh (committee member), Chai-Lin Kao (chair).
Subjects/Keywords: alcohol; amyloid; pH; peptide; insulin
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APA ·
Chicago ·
MLA ·
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Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Huang, H. (2013). Influence of chemical conditions on human insulin fibril structure. (Thesis). NSYSU. Retrieved from http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0713113-113753
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Huang, Huei-Jhen. “Influence of chemical conditions on human insulin fibril structure.” 2013. Thesis, NSYSU. Accessed September 19, 2019.
http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0713113-113753.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Huang, Huei-Jhen. “Influence of chemical conditions on human insulin fibril structure.” 2013. Web. 19 Sep 2019.
Vancouver:
Huang H. Influence of chemical conditions on human insulin fibril structure. [Internet] [Thesis]. NSYSU; 2013. [cited 2019 Sep 19].
Available from: http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0713113-113753.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Huang H. Influence of chemical conditions on human insulin fibril structure. [Thesis]. NSYSU; 2013. Available from: http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0713113-113753
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
University of Akron
29.
Liu, Qianhui.
Structure-Property Relationship of "Peptide-like"
Polyesters.
Degree: MS, Polymer Science, 2015, University of Akron
URL: http://rave.ohiolink.edu/etdc/view?acc_num=akron1428336910
► Polyesters are wildly used materials in biomedical field such as drug delivery material, tissue engineering scaffolds material such as for bone regeneration and so on.…
(more)
▼ Polyesters are wildly used materials in biomedical
field such as drug delivery material, tissue engineering scaffolds
material such as for bone regeneration and so on. However, because
of complexity of internal environment, traditional polyesters like
poly(lactic-co-glycolic acid) (PLGA), Polycaprolactone (PCL) and
Polypropylene Fumarate (PPF), their properties are quite difficult
to be easily tuned to accomplish specific application. Changing
chemical structures is a method to modulate polymer properties. In
Dr. Joy’s lab, a series of polyesters with “
peptide-like” pendant
functional groups was synthesized at room temperature via
carbodiimide-mediated polymerization of pendant functionalized
diols and diacid by Sachin Gokhale and Ying Xu. On this basis,
polyesters with different types of pendant functionalized groups
and diacids were synthesized, besides, hydrogen bonding was
introduced into polymer side chains and the sequence of monomer
addition was studied. Polymers were characterized with NMR, GPC,
DSC, TGA, water contact angle water hydrolytic rate thus their
physical properties can be understood and compared due to the
change of structures. Besides, rheology tests and mechanical tests
were carried out to further study the differences between various
polymers. As biomaterial, cell compatibility of the synthesized
polyester will be tested in the future to study whether the
material is available in internal environment.
Advisors/Committee Members: Joy, Abraham (Advisor).
Subjects/Keywords: Polymers; polyesters, peptide-like, structures
Record Details
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Record Details
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APA ·
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APA (6th Edition):
Liu, Q. (2015). Structure-Property Relationship of "Peptide-like"
Polyesters. (Masters Thesis). University of Akron. Retrieved from http://rave.ohiolink.edu/etdc/view?acc_num=akron1428336910
Chicago Manual of Style (16th Edition):
Liu, Qianhui. “Structure-Property Relationship of "Peptide-like"
Polyesters.” 2015. Masters Thesis, University of Akron. Accessed September 19, 2019.
http://rave.ohiolink.edu/etdc/view?acc_num=akron1428336910.
MLA Handbook (7th Edition):
Liu, Qianhui. “Structure-Property Relationship of "Peptide-like"
Polyesters.” 2015. Web. 19 Sep 2019.
Vancouver:
Liu Q. Structure-Property Relationship of "Peptide-like"
Polyesters. [Internet] [Masters thesis]. University of Akron; 2015. [cited 2019 Sep 19].
Available from: http://rave.ohiolink.edu/etdc/view?acc_num=akron1428336910.
Council of Science Editors:
Liu Q. Structure-Property Relationship of "Peptide-like"
Polyesters. [Masters Thesis]. University of Akron; 2015. Available from: http://rave.ohiolink.edu/etdc/view?acc_num=akron1428336910
Ruhr Universität Bochum
30.
Penkova, Maya.
Arginine and Tryptophan rich antimicrobial peptides
(AMPs) : modifications, application and mode of action.
Degree: 2010, Ruhr Universität Bochum
URL: http://nbn-resolving.de/urn/resolver.pl?urn=urn:nbn:de:hbz:294-30249
► Da multiresistente Bakterienstämme ein häufiges Problem darstellen, besteht Bedarf an neuen Verbindungen, die keine Resistenzen hervorrufen. Eine solche Verbindungsklasse stellen die kationischen antimikrobiellen Peptide dar…
(more)
▼ Da multiresistente Bakterienstämme ein häufiges
Problem darstellen, besteht Bedarf an neuen Verbindungen, die keine
Resistenzen hervorrufen. Eine solche Verbindungsklasse stellen die
kationischen antimikrobiellen
Peptide dar (cationic antimicrobial
peptides, AMPs). Mithilfe von Festphasenpeptidsynthese wurden
Peptide und deren Metallocenanaloga (Ferrocen- und
Ruthenocenbiokonjugate) hergestellt und auf ihre biologische
Aktivität untersucht. Alle hergestellten Verbindungen zeigten
antimikrobielle Eigenschaften. Um den Wirkmechanismus aufzuklären,
wurden MudPit-Analysen und Proteomik verwendet. Nach der Behandlung
von B. Subtilis mit AMPs war dessen Proteinprofil vergleichbar zu
dem wie nach der Behandlung mit Triton X-100. Desweiteren wurden
Proteinmarker gefunden wie bei Valinomycin und Bacitracin. Diese
Ergebnisse weisen darauf hin, dass das Target der AMPs die
Zytoplasmamembran ist.
Advisors/Committee Members: Chemie.
Subjects/Keywords: Arginin; Tryptophan; Ferrocen; Peptide
Record Details
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Cite
Share »
Record Details
Similar Records
Cite
« Share
❌
APA ·
Chicago ·
MLA ·
Vancouver ·
CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Penkova, M. (2010). Arginine and Tryptophan rich antimicrobial peptides
(AMPs) : modifications, application and mode of action. (Thesis). Ruhr Universität Bochum. Retrieved from http://nbn-resolving.de/urn/resolver.pl?urn=urn:nbn:de:hbz:294-30249
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Penkova, Maya. “Arginine and Tryptophan rich antimicrobial peptides
(AMPs) : modifications, application and mode of action.” 2010. Thesis, Ruhr Universität Bochum. Accessed September 19, 2019.
http://nbn-resolving.de/urn/resolver.pl?urn=urn:nbn:de:hbz:294-30249.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Penkova, Maya. “Arginine and Tryptophan rich antimicrobial peptides
(AMPs) : modifications, application and mode of action.” 2010. Web. 19 Sep 2019.
Vancouver:
Penkova M. Arginine and Tryptophan rich antimicrobial peptides
(AMPs) : modifications, application and mode of action. [Internet] [Thesis]. Ruhr Universität Bochum; 2010. [cited 2019 Sep 19].
Available from: http://nbn-resolving.de/urn/resolver.pl?urn=urn:nbn:de:hbz:294-30249.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Penkova M. Arginine and Tryptophan rich antimicrobial peptides
(AMPs) : modifications, application and mode of action. [Thesis]. Ruhr Universität Bochum; 2010. Available from: http://nbn-resolving.de/urn/resolver.pl?urn=urn:nbn:de:hbz:294-30249
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
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Open Access Theses and Dissertations
