subject:(pathogen derived resistance)

You searched for subject:(pathogen derived resistance). Showing records 1 – 10 of 10 total matches.

▼ Search Limiters

Stellenbosch University

1. Suidgeest, Faira. Evaluation of two pathogen-derived resistance strategies for Grapevine leafroll-associated virus 3.

Degree: MSc, Genetics, 2015, Stellenbosch University

URL: http://hdl.handle.net/10019.1/96776

►

ENGLISH ABSTRACT: Grapevine leafroll disease (GLD), caused by the members of the family Closteroviridae, is one of the most economic important viral diseases affecting grapevine.… (more)

▼

ENGLISH ABSTRACT: Grapevine leafroll disease (GLD), caused by the members of the family Closteroviridae, is one of the most economic important viral diseases affecting grapevine. Grapevine leafroll associated virus 3 (GLRaV-3), of the genus Ampelovirus, is the most widespread member of the leafroll associated virus family. To prevent the spread of GLD, management strategies such as rogueing and insect vector control are required to limit crop losses. Alternative control strategies based on genetic modification of the grapevine genome, such as pathogen-derived resistance (PDR), is proven to be effective in conferring resistance to several viruses. Therefore, the focus of this study was to evaluate pathogen-derived resistance strategies for GLRaV-3 using the following two approaches; 1) evaluation of transgenic plants expressing a dysfunctional GLRaV-3 heat shock protein 70 homolog (HSP70h) in order to confer resistance against GLRaV-3, and 2) the construction of artificial microRNAs (amiRNAs) to use as a tool for silencing specific sequences of GLRaV-3 in the grapevine host and the development of an amiRNA-mediated silencing validation system. In the first part of this study, six transgenic plant lines (plant lines1, #3, #9, #14, #15 and #17) as well as a non-modified plant line, were inoculated with GLRaV-3 by grafting buds of each onto GLRaV-3 infected plant material. After approximately five months, GLRaV-3 virus titres of all grafted plants were quantified relative to two reference genes using RT-qPCR. Results were evaluated by comparing the relative virus titre of each transgenic plant line to that of the non-modified control plant line. Results showed that resistance levels of plant line #3 was significantly enhanced (>99%) and remarkably, plant line #14, showed to be more susceptible to the virus. The second part of the study was the construction and validation of amiRNAs targeting GLRaV-3 sequences. Two 21 nt regions of GLRaV-3 were successfully incorporated into miRNA backbone vvi167b of grapevine. Moreover, target constructs were developed by incorporating corresponding GLRaV-3 target sequences into the 3’ UTR of a green fluorescence protein (GFP) gene. Subsequently, the target constructs were co-infiltrated with the constructed amiRNA in Nicotiana benthamiana and GFP expression levels were quantified to determine the silencing efficiency of the amiRNAs. Results showed that the amiRNAs were successful in silencing the GFP target construct, however, they were not specific in silencing exclusively their corresponding target. These amiRNA constructs are ideal for further viral studies to determine the efficiency of silencing GLRaV-3 in GLD infected grapevines.

AFRIKAANSE OPSOMMING: Wingerd rolblaar siekte (GLD), wat veroorsaak word deur die lede van die familie Closteroviridae, is een van die ekonomies mees belangrike virus siektes van wingerd. Grapevine leafroll-associated virus 3 (GLRaV-3), van die genus Ampelovirus, is die mees wydverspreide lid van die rolblaar…

Advisors/Committee Members: Burger, Johan T., Maree, Hans Jacob, Stellenbosch University. Faculty of Agrisciences. Dept. of Genetics..

Subjects/Keywords: Grapevine leafroll virus; Grapes – Disease and pest resistance; Pathogen-derived resistance; Virus diseases of plants; UCTD

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Suidgeest, F. (2015). Evaluation of two pathogen-derived resistance strategies for Grapevine leafroll-associated virus 3. (Masters Thesis). Stellenbosch University. Retrieved from http://hdl.handle.net/10019.1/96776

Chicago Manual of Style (16^th Edition):

Suidgeest, Faira. “Evaluation of two pathogen-derived resistance strategies for Grapevine leafroll-associated virus 3.” 2015. Masters Thesis, Stellenbosch University. Accessed February 28, 2021. http://hdl.handle.net/10019.1/96776.

MLA Handbook (7^th Edition):

Suidgeest, Faira. “Evaluation of two pathogen-derived resistance strategies for Grapevine leafroll-associated virus 3.” 2015. Web. 28 Feb 2021.

Vancouver:

Suidgeest F. Evaluation of two pathogen-derived resistance strategies for Grapevine leafroll-associated virus 3. [Internet] [Masters thesis]. Stellenbosch University; 2015. [cited 2021 Feb 28]. Available from: http://hdl.handle.net/10019.1/96776.

Council of Science Editors:

Suidgeest F. Evaluation of two pathogen-derived resistance strategies for Grapevine leafroll-associated virus 3. [Masters Thesis]. Stellenbosch University; 2015. Available from: http://hdl.handle.net/10019.1/96776

Queensland University of Technology

2. Williams, Brett Robert. Development of a novel rep-inducible tomato leaf curl virus expression system.

Degree: 2007, Queensland University of Technology

URL: https://eprints.qut.edu.au/16539/

► Pathogen-derived resistance (PDR) strategies, particularly those based on post-transcriptional gene silencing, have been used with great success for the generation of transgenic plants with resistance… (more)

▼ Pathogen-derived resistance (PDR) strategies, particularly those based on post-transcriptional gene silencing, have been used with great success for the generation of transgenic plants with resistance to RNA viruses. In contrast, a suitable strategy for transgenic resistance to ssDNA plant viruses, including those viruses belonging to the Geminiviridae, has remained elusive. Further, there is no convincing evidence that either post-transcriptional gene silencing, or pathogen-derived resistance in general, would be broadly applicable to ssDNA plant viruses. Researchers at QUT have been developing a novel resistance strategy against ssDNA viruses based on virus-activated expression of a stably integrated suicide gene. The strategy, based on InPAct (In Plant Activation) technology, relies on a "split" suicide gene cassette being arranged in such a way that expression of a lethal ribonuclease (barnase) is dependent on the virus-encoded replication-associated protein (Rep). Upon infection, Rep mediates the release of the construct resulting in the reconstitution of a transcribable and translatable episomal suicide gene expression cassette. The research for this PhD describes the development of an InPAct vector designed to confer resistance to Tomato leaf curl begomovirus (ToLCV), a major cause of disease in Solanaceous crops in the tropics and subtropics. ToLCV-based InPAct vectors were constructed based upon two ToLCV isolates from Australia and North Vietnam. Prior to the generation of InPAct cassettes, the entire ToLCV-[Au] and ToLCV-Vie intergenic regions (IRs) were embedded within the castorbean catalase intron of a β-glucuronidase expression vector to determine the effect of the IR upon transcript processing. Using transient reporter gene assays in tobacco NT-1 cells, it was demonstrated that the ToLCV IRs both contained cryptic intron splice sites which interfered with efficient transcript processing and GUS expression. A series of truncations to the IRs were subsequently made to identify the potential cryptic intron splice sites and/or interfering sequences in both the ToLCV-[Au] and ToLCV-Vie IRs. The final truncated IRs, which were used in the construction the InPAct cassettes, comprised approximately 100 bp and appeared to contain all the necessary cis-acting elements required for efficient rolling circle replication (RCR). Using histochemical GUS assays and Southern analyses, the InPAct cassettes were shown to be activated and replicated only in the presence of the cognate viral Rep. GUS expression levels were shown to be further enhanced in the presence of the ToLCV replication-enhancer protein (REn) and by the addition of the Tobacco yellow dwarf mastrevirus origin of second strand synthesis into the cassette. Under these conditions, Rep-activated GUS expression from the InPAct vectors was found to reach levels similar to that of the benchmark CaMV 35S promoter. Fifteen independent transgenic lines containing the ToLCV-[Au] and -Vie InPAct-GUS cassettes were generated by…

Subjects/Keywords: pathogen-derived resistance (PDR); tomato leaf curl virus; replication-association protein (Rep); begomoviruses

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Williams, B. R. (2007). Development of a novel rep-inducible tomato leaf curl virus expression system. (Thesis). Queensland University of Technology. Retrieved from https://eprints.qut.edu.au/16539/

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Williams, Brett Robert. “Development of a novel rep-inducible tomato leaf curl virus expression system.” 2007. Thesis, Queensland University of Technology. Accessed February 28, 2021. https://eprints.qut.edu.au/16539/.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Williams, Brett Robert. “Development of a novel rep-inducible tomato leaf curl virus expression system.” 2007. Web. 28 Feb 2021.

Vancouver:

Williams BR. Development of a novel rep-inducible tomato leaf curl virus expression system. [Internet] [Thesis]. Queensland University of Technology; 2007. [cited 2021 Feb 28]. Available from: https://eprints.qut.edu.au/16539/.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Williams BR. Development of a novel rep-inducible tomato leaf curl virus expression system. [Thesis]. Queensland University of Technology; 2007. Available from: https://eprints.qut.edu.au/16539/

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Queensland University of Technology

3. McQualter, Richard Bruce. Production and evaluation of transgenic sugarcane plants with pathogen-derived resistance to Fiji disease virus.

Degree: 2003, Queensland University of Technology

URL: http://eprints.qut.edu.au/37162/

Subjects/Keywords: Sugarcane Diseases and pests; Fiji disease virus; saccharum; sugarcane; transgene; pathogen-derived resistance; Fiji disease virus; reovirus; thesis; doctoral

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

McQualter, R. B. (2003). Production and evaluation of transgenic sugarcane plants with pathogen-derived resistance to Fiji disease virus. (Thesis). Queensland University of Technology. Retrieved from http://eprints.qut.edu.au/37162/

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

McQualter, Richard Bruce. “Production and evaluation of transgenic sugarcane plants with pathogen-derived resistance to Fiji disease virus.” 2003. Thesis, Queensland University of Technology. Accessed February 28, 2021. http://eprints.qut.edu.au/37162/.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

McQualter, Richard Bruce. “Production and evaluation of transgenic sugarcane plants with pathogen-derived resistance to Fiji disease virus.” 2003. Web. 28 Feb 2021.

Vancouver:

McQualter RB. Production and evaluation of transgenic sugarcane plants with pathogen-derived resistance to Fiji disease virus. [Internet] [Thesis]. Queensland University of Technology; 2003. [cited 2021 Feb 28]. Available from: http://eprints.qut.edu.au/37162/.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

McQualter RB. Production and evaluation of transgenic sugarcane plants with pathogen-derived resistance to Fiji disease virus. [Thesis]. Queensland University of Technology; 2003. Available from: http://eprints.qut.edu.au/37162/

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Texas A&M University

4. Herron, Caroline Mary. Citrus tristeza virus: characterization of Texas isolates, studies on aphid transmission and pathogen-derived control strategies.

Degree: PhD, Plant Pathology, 2004, Texas A&M University

URL: http://hdl.handle.net/1969.1/1198

► Citrus tristeza virus (CTV), an economically important graft-transmissible pathogen of citrus, causes major global declines in citrus production. In the commercial citrus of the Lower… (more)

▼ Citrus tristeza virus (CTV), an economically important graft-transmissible pathogen of citrus, causes major global declines in citrus production. In the commercial citrus of the Lower Rio Grande Valley of Texas (LRGV), where red grapefruit on tristeza-decline sensitive sour orange rootstocks predominate, incidence of CTV is low. The efficient CTV vector, the brown citrus aphid (BrCA, Toxoptera citricida Kirkaldy) is now established in Mexico and Florida, thus information is needed on the severity of CTV, CTV aphid transmission and the performance of transformed citrus towards CTV before T. citricida arrives in Texas so that appropriate management strategies can be selected. Biological indexing and molecular typing were performed on fifteen Texas CTV isolates. The majority of the CTV isolates tested contained the most severe CTV types known. In Florida, T. citricida were fed on crude CTV preparations in vitro and could transmit CTV to virus-free receptor plants with two CTV isolates, whereas a more highly purified CTV preparation from one CTV isolate was not transmitted by T. citricida. There were no differences in the majority of treatments in infectivity neutralizations using three CTV-derived antibodies (p25, p27 and p20). CTV p20 antibodies significantly enhanced the occurrence of CTV transmission in one test. The CTV genome of isolate H33 was sequenced using 'shot gun' methods. The H33 major component and H33 minor components were phylogenetically compared to the six other full-length CTV sequences. An untranslatable CTV coat protein gene was genetically transformed into the genome of the Texas commercial Rio Red grapefruit variety, and fifty-two independent transgenic lines were produced. CTV challenge responses by the transgenic lines were variable. Individual plants could be identified which had low virus titers by ELISA detection, a temporal decrease in virus titer, or a delay in virus titer accumulation. Comparing all wild types to all transgenic lines over every assessment revealed significant decreases in virus titer in the transgenic lines compared to that of the wild type. An RNA entity with similarities to marafiviruses was identified in a CTV infected plant. The entity appears non-graft transmissible to citrus, and non-mechanically transmissible to a range of herbaceous species. Advisors/Committee Members: Scholthof, Herman (advisor), da Graca, John (advisor), Scholthof, Karen-Beth (committee member), Lee, Richard (committee member), Mirkov, T. Erik (committee member).

Subjects/Keywords: Citrus tristeza virus; Toxoptera citricida; the brown citrus aphid; transgenic citrus; pathogen-derived resistance; marafiviruses

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Herron, C. M. (2004). Citrus tristeza virus: characterization of Texas isolates, studies on aphid transmission and pathogen-derived control strategies. (Doctoral Dissertation). Texas A&M University. Retrieved from http://hdl.handle.net/1969.1/1198

Chicago Manual of Style (16^th Edition):

Herron, Caroline Mary. “Citrus tristeza virus: characterization of Texas isolates, studies on aphid transmission and pathogen-derived control strategies.” 2004. Doctoral Dissertation, Texas A&M University. Accessed February 28, 2021. http://hdl.handle.net/1969.1/1198.

MLA Handbook (7^th Edition):

Herron, Caroline Mary. “Citrus tristeza virus: characterization of Texas isolates, studies on aphid transmission and pathogen-derived control strategies.” 2004. Web. 28 Feb 2021.

Vancouver:

Herron CM. Citrus tristeza virus: characterization of Texas isolates, studies on aphid transmission and pathogen-derived control strategies. [Internet] [Doctoral dissertation]. Texas A&M University; 2004. [cited 2021 Feb 28]. Available from: http://hdl.handle.net/1969.1/1198.

Council of Science Editors:

Herron CM. Citrus tristeza virus: characterization of Texas isolates, studies on aphid transmission and pathogen-derived control strategies. [Doctoral Dissertation]. Texas A&M University; 2004. Available from: http://hdl.handle.net/1969.1/1198

Queensland University of Technology

5. Tsao, Theresa Tsun-Hui. Towards the development of transgenic banana bunchy top virus (BBTV)-resistant banana plants : interference with replication.

Degree: 2008, Queensland University of Technology

URL: https://eprints.qut.edu.au/17031/

► Banana bunchy top virus (BBTV) causes one of the most devastating diseases of banana. Transgenic virus resistance is now considered one of the most promising… (more)

▼ Banana bunchy top virus (BBTV) causes one of the most devastating diseases of banana. Transgenic virus resistance is now considered one of the most promising strategies to control BBTV. Pathogen-derived resistance (PDR) strategies have been applied successfully to generate plants that are resistant to numerous different viruses, primarily against those viruses with RNA genomes. BBTV is a circular, single-stranded (css) DNA virus of the family Nanoviridae, which is closely related to the family Geminiviridae. Although there are some successful examples of PDR against geminiviruses, PDR against the nanoviruses has not been reported. Therefore, the aim of this thesis was to investigate the potential of BBTV genes to interfere with virus replication when used as transgenes for engineering banana plants resistance to BBTV. The replication initiation protein (Rep) of nanoviruses is the only viral protein essential for viral replication and represents an ideal target for PDR. Therefore, this thesis focused on the effect of wild-type or mutated Rep genes from BBTV satellite DNAs or the BBTV integral genome on the replication of BBTV in banana embryogenic cell suspensions. A new Rep-encoding satellite DNA, designated BBTV DNA-S4, was isolated from a Vietnamese BBTV isolate and characterised. When the effect of DNA-S4 on the replication of BBTV was examined, it was found that DNA-S4 enhanced the replication of BBTV. When the replicative capabilities of DNA-S4 and the previously characterised Rep-encoding BBTV satellite, DNA-S1, were compared, it was found that the amount of DNA-S4 accumulated to higher levels than DNA-S1. The interaction between BBTV and DNA-S1 was also examined. It was found that over-expression of the Rep encoded by DNA-S1 using ubi1 maize polyubiquitin promoter enhanced replication of BBTV. However, when the Rep-encoded by DNA-S1 was expressed by the native S1 promoter (in plasmid pBT1.1-S1), it suppressed the replication of BBTV. Based on this result, the use of DNA-S1 as a possible transgene to generate PDR against BBTV was investigated. The roles of the Rep-encoding and U5 genes of BBTV DNA-R, and the effects of over-expression of these two genes on BBTV replication were also investigated. Three mutants of BBTV DNA-R were constructed; plasmid pUbi-RepOnly-nos contained the ubi1 promoter driving Rep expression from DNA-R, plasmid pUbi-IntOnly-nos contained the ubi1 promoter driving expression of the DNA-R internal gene product (U5), while plasmid pUbi-R.ORF-nos contained the ubi1 promoter driving the expression of both Rep and the internal U5 gene product. The replication of BBTV was found to be significantly suppressed by pUbi-RepOnly-nos, weakly suppressed by pUbi-IntOnly-nos, but strongly enhanced by pUbi-R.ORF-nos. The effect of mutations in three conserved residues within the BBTV Rep on BBTV replication was also assessed. These mutations were all made in the regions in the ATPase motifs and resulted in changes from hydrophilic to hydrophobic residues (i.e. K187→M, D224→I and N268→L). None of…

Subjects/Keywords: banana bunchy top virus; nanoviruses; replication initiation protein (Rep); pathogen-derived resistance; BBTV DNA-R; BBTV DNA-S1; BBTV DNA-S3; BBTV DNA-S4; satellite DNA; ATPase; post-transcriptional gene silencing; RNA silencing suppressor

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Tsao, T. T. (2008). Towards the development of transgenic banana bunchy top virus (BBTV)-resistant banana plants : interference with replication. (Thesis). Queensland University of Technology. Retrieved from https://eprints.qut.edu.au/17031/

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Tsao, Theresa Tsun-Hui. “Towards the development of transgenic banana bunchy top virus (BBTV)-resistant banana plants : interference with replication.” 2008. Thesis, Queensland University of Technology. Accessed February 28, 2021. https://eprints.qut.edu.au/17031/.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Tsao, Theresa Tsun-Hui. “Towards the development of transgenic banana bunchy top virus (BBTV)-resistant banana plants : interference with replication.” 2008. Web. 28 Feb 2021.

Vancouver:

Tsao TT. Towards the development of transgenic banana bunchy top virus (BBTV)-resistant banana plants : interference with replication. [Internet] [Thesis]. Queensland University of Technology; 2008. [cited 2021 Feb 28]. Available from: https://eprints.qut.edu.au/17031/.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Tsao TT. Towards the development of transgenic banana bunchy top virus (BBTV)-resistant banana plants : interference with replication. [Thesis]. Queensland University of Technology; 2008. Available from: https://eprints.qut.edu.au/17031/

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Brigham Young University

6. Cobb, Joshua Nathaniel. Studies on Transformation of Tomato(Solanum lycopersicum L.) and Arabidopsis thaliana using Chimerical constructs of varying Tospoviral Origin.

Degree: MA, 2008, Brigham Young University

URL: https://scholarsarchive.byu.edu/cgi/viewcontent.cgi?article=2477&context=etd

► Pathogen derived resistance (PDR) is a recent breakthrough where plant hosts can be made to be resistant to viral infections through transformation with conserved… (more)

▼ Pathogen derived resistance (PDR) is a recent breakthrough where plant hosts can be made to be resistant to viral infections through transformation with conserved viral genes. Given the severity of Tospovirus diseases worldwide (particularly in tomato), PDR has the potential to garner large yield returns where pathogen populations have overcome the established resistance. Tomato breeding lines FLA7804, FLA8044, and the research line MP1 were used in transformation experiments with potions of the Tomato spotted wilt virus (TSWV) N-gene, and two other chimerical viral nucleocapsid gene constructs from, Impatiens necrotic spot virus (INSV), and Groundnut ringspot virus (GRSV). We conducted 19 independent transformations consisting of 300 to 700 14-day old whole cotyledons each for a total number of approximately 9,000 potentially transformed explants. Of those, approximately 6,300 explants failed to produce regenerants, 2,419 explants underwent abnormal development on elongation media, 187 failed to root, and 215 plants to be characterized genetically. Of the 215 plants, 9 were from FLA 7804, 96 from FLA 8044, and 110 from MP1. Both PCR and Southern blot hybridization analysis later confirmed that none of the 215 plants were transgenic. Opposite to tomato, we were able to transform Arabidopsis thaliana ecotype wassilewskija (Ws) via floral dip with the above listed constructs demonstrating that constructs were not deleterious within a plant once fully introgressed. Sixteen independent transformants in the T0 generation resulted from 19,000 germinated seed from three dipped plants resulting in a total transformation rate of 0.08%. Of the 1,000 T1 seed germinated on kanamycin media from each of the 16 putative Arabidopsis plants transformed with the construct containing elements of the N-gene from all three of the aforementioned tospoviruses, four populations exhibited simple Mendelian inheritance of the transgene. DNA walking analysis yielded amplification of the unknown region outside the nptII region of the insert for three of the four remaining transformants, which was subsequently sequenced and mapped to chromosomes 1, 3, and 4. There were 25 T1 individuals selected from each population and transferred to soil for DNA extraction and zygosity determination. Homozygous T2 seed was collected for future resistance studies.

Subjects/Keywords: tomato; TSWV; Tospovirus; pathogen derived resistance; PDR; plant transformation; Solanum lycopersicum; Arabidopsis thaliana; Animal Sciences

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Cobb, J. N. (2008). Studies on Transformation of Tomato(Solanum lycopersicum L.) and Arabidopsis thaliana using Chimerical constructs of varying Tospoviral Origin. (Masters Thesis). Brigham Young University. Retrieved from https://scholarsarchive.byu.edu/cgi/viewcontent.cgi?article=2477&context=etd

Chicago Manual of Style (16^th Edition):

Cobb, Joshua Nathaniel. “Studies on Transformation of Tomato(Solanum lycopersicum L.) and Arabidopsis thaliana using Chimerical constructs of varying Tospoviral Origin.” 2008. Masters Thesis, Brigham Young University. Accessed February 28, 2021. https://scholarsarchive.byu.edu/cgi/viewcontent.cgi?article=2477&context=etd.

MLA Handbook (7^th Edition):

Cobb, Joshua Nathaniel. “Studies on Transformation of Tomato(Solanum lycopersicum L.) and Arabidopsis thaliana using Chimerical constructs of varying Tospoviral Origin.” 2008. Web. 28 Feb 2021.

Vancouver:

Cobb JN. Studies on Transformation of Tomato(Solanum lycopersicum L.) and Arabidopsis thaliana using Chimerical constructs of varying Tospoviral Origin. [Internet] [Masters thesis]. Brigham Young University; 2008. [cited 2021 Feb 28]. Available from: https://scholarsarchive.byu.edu/cgi/viewcontent.cgi?article=2477&context=etd.

Council of Science Editors:

Cobb JN. Studies on Transformation of Tomato(Solanum lycopersicum L.) and Arabidopsis thaliana using Chimerical constructs of varying Tospoviral Origin. [Masters Thesis]. Brigham Young University; 2008. Available from: https://scholarsarchive.byu.edu/cgi/viewcontent.cgi?article=2477&context=etd

Swedish University of Agricultural Sciences

7. Melander, Margareta. Transgenic resistance to pathogens and pests.

Degree: 2004, Swedish University of Agricultural Sciences

URL: http://pub.epsilon.slu.se/687/

► Pathogens and pests constantly threaten plants and cause crop losses of significant economic importance for agricultural production worldwide. One way to reduce the damage caused… (more)

▼ Pathogens and pests constantly threaten plants and cause crop losses of significant economic importance for agricultural production worldwide. One way to reduce the damage caused by pathogens and pests is the development of new, resistant cultivars. However, conventional resistance breeding often suffers from limited access to suitable resistance sources. The development of gene technology has drastically increased the availability of genes conferring resistance, which can be derived from non-related plant species as well as non-plant sources. In this thesis three attempts to improve the resistance against a virus, some fungi and an insect by genetic engineering are described. In the first example an approach to develop potato with improved resistance against Potato mop-top virus was evaluated. A modified version of a gene involved in viral movement from Potato mop-top virus was transformed into potato. Transgenic plants shown to transcribe the inserted sequence were evaluated in a field trial, where increased resistance to natural infection by Potato mop-top virus could be demonstrated. The second example is the evaluation of doubled haploids of oilseed rape transformed with a chitinase and a b-1,3-glucanase gene from barley. Although the barley chitinase and b-1,3-glucanase did show some antifungal effects when evaluated in vitro, no significant effects could be demonstrated when the transgenic plants were challenged with four different oilseed rape fungi in greenhouse assays. The doubled haploids were also evaluated for stability of the transgenic inserts as well as their expression during five subsequent generations. The third example evaluates pea lectin as a possible resistance factor against pollen beetles in oilseed rape. In feeding assays with pollen beetle larvae several plant proteins with potential insecticidal activity were tested. In these assays pea lectin was shown to have significant detrimental effects both on growth and survival of the larvae. Therefore, transgenic oilseed rape plants were produced that expressed pea lectin in pollen. When pollen beetle larvae were fed anthers from these plants significant reduction in larval weight was observed as well as some effect on survival.

Subjects/Keywords: fungal diseases; pests of plants; pest resistance; pest insects; viruses; genetic transformation; disease resistance; genetic engineering; fungal resistance; insect resistance; molecular breeding; pathogen-derived resistance; PR proteins; transformation; virus resistance

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Melander, M. (2004). Transgenic resistance to pathogens and pests. (Doctoral Dissertation). Swedish University of Agricultural Sciences. Retrieved from http://pub.epsilon.slu.se/687/

Chicago Manual of Style (16^th Edition):

Melander, Margareta. “Transgenic resistance to pathogens and pests.” 2004. Doctoral Dissertation, Swedish University of Agricultural Sciences. Accessed February 28, 2021. http://pub.epsilon.slu.se/687/.

MLA Handbook (7^th Edition):

Melander, Margareta. “Transgenic resistance to pathogens and pests.” 2004. Web. 28 Feb 2021.

Vancouver:

Melander M. Transgenic resistance to pathogens and pests. [Internet] [Doctoral dissertation]. Swedish University of Agricultural Sciences; 2004. [cited 2021 Feb 28]. Available from: http://pub.epsilon.slu.se/687/.

Council of Science Editors:

Melander M. Transgenic resistance to pathogens and pests. [Doctoral Dissertation]. Swedish University of Agricultural Sciences; 2004. Available from: http://pub.epsilon.slu.se/687/

8. Rupp, Jessica Lynn Shoup. RNA interference mediated virus resistance in transgenic wheat.

Degree: PhD, Plant Pathology, 2015, Kansas State University

URL: http://hdl.handle.net/2097/20387

► Wheat streak mosaic virus (WSMV) and Triticum mosaic virus (TriMV) are two viruses affecting wheat in the Great Plains region of the United States. Genetic… (more)

▼ Wheat streak mosaic virus (WSMV) and Triticum mosaic virus (TriMV) are two viruses affecting wheat in the Great Plains region of the United States. Genetic resistance is severely limited, requiring management methods focusing on the deployment of resistant varieties and various cultural practices. Evaluation of resistance is complicated by the lack of a standard rating scale. The objective of this work was to develop new avenues to mitigate these challenges. A standardized virus symptom rating scale was developed using historical Kansas rating scales, and validated using multiple wheat populations. Two independent RNA interference (RNAi) expression vectors targeting portions of viral coat protein (CP) of WSMV and TriMV were previously transformed into wheat. T₂ plants and beyond were evaluated using PCR, reverse transcription-PCR and bioassays in which plants were challenged with their respective virus. These lines were evaluated for resistance through the T₆ generation. Crosses were made with the susceptible winter wheat cultivars, ‘Overley’ and ‘Karl 92.’ Real-time PCR results show viral titer was up to 20-fold lower in the T₆ transgenic lines, the F₁, and the BC₁F₁ compared to control plants. This provides evidence that this RNAi silencing method is stable in wheat over multiple generations. WSMV and TriMV use host eukaryotic initiation factors (eIF) in order to facilitate replication of their genomes. Previously created RNAi expression vectors were derived from the sequences of the wheat genes eIF(iso)4E-2 and eIF4G. Evaluation of these lines began in the T₁ generation. Resistance has been demonstrated in three lines of eIF(iso)4E-2 and four lines of eIF4G, derived by single seed descent. T₆ progeny co-infected with WSMV and TriMV continue to be resistant. Crosses have been performed with the winter wheat ‘Karl 92’ and three Kansas elite lines, KS030887K-6, KS09H19-2-3, and KS10HW78-1-1. RNAi construct effectiveness was evaluated using real-time PCR. Results show up to 18-fold reduction in viral titer in the transgenic lines, the F₁, and the BC₁F₁ in comparison to control plants. This research provides the first evidence that a single host transgene can provide resistance to multiple viruses and has great potential benefits to both breeders and producers. Advisors/Committee Members: John P. FellersHarold N. Trick.

Subjects/Keywords: Transgenic wheat; Virus resistance; Host gene silencing; Pathogen derived resistance; RNA Interference; Virus rating scale; Plant Pathology (0480)

…have proven applicable to potyvirus and their host systems. WSMV pathogen derived resistance… …18 Chapter Two - Stable resistance to Wheat streak mosaic virus in wheat mediated by RNAi… …47 Chapter Four - RNAi mediated stable resistance to Triticum mosaic virus… …and eIF4G induce resistance to multiple RNA viruses in transgenic wheat… …36 Figure 2.4 Analysis of T1 plants derived from T0 transgenic individuals…

10 more images…

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Rupp, J. L. S. (2015). RNA interference mediated virus resistance in transgenic wheat. (Doctoral Dissertation). Kansas State University. Retrieved from http://hdl.handle.net/2097/20387

Chicago Manual of Style (16^th Edition):

Rupp, Jessica Lynn Shoup. “RNA interference mediated virus resistance in transgenic wheat.” 2015. Doctoral Dissertation, Kansas State University. Accessed February 28, 2021. http://hdl.handle.net/2097/20387.

MLA Handbook (7^th Edition):

Rupp, Jessica Lynn Shoup. “RNA interference mediated virus resistance in transgenic wheat.” 2015. Web. 28 Feb 2021.

Vancouver:

Rupp JLS. RNA interference mediated virus resistance in transgenic wheat. [Internet] [Doctoral dissertation]. Kansas State University; 2015. [cited 2021 Feb 28]. Available from: http://hdl.handle.net/2097/20387.

Council of Science Editors:

Rupp JLS. RNA interference mediated virus resistance in transgenic wheat. [Doctoral Dissertation]. Kansas State University; 2015. Available from: http://hdl.handle.net/2097/20387

Queensland University of Technology

9. Becker, Douglas Kenneth. The transformation of banana with potential virus resistance genes.

Degree: 1999, Queensland University of Technology

URL: https://eprints.qut.edu.au/37023/

► One approach to reducing the yield losses caused by banana viral diseases is the use of genetic engineering and pathogen-derived resistance strategies to generate resistant… (more)

▼ One approach to reducing the yield losses caused by banana viral diseases is the use of genetic engineering and pathogen-derived resistance strategies to generate resistant cultivars. The development of transgenic virus resistance requires an efficient banana transformation method, particularly for commercially important 'Cavendish' type cultivars such as 'Grand Nain'. Prior to this study, only two examples of the stable transformation of banana had been reported, both of which demonstrated the principle of transformation but did not characterise transgenic plants in terms of the efficiency at which individual transgenic lines were generated, relative activities of promoters in stably transformed plants, and the stability of transgene expression. The aim of this study was to develop more efficient transformation methods for banana, assess the activity of some commonly used and also novel promoters in stably transformed plants, and transform banana with genes that could potentially confer resistance to banana bunchy top nanovirus (BBTV) and banana bract mosaic potyvirus (BBrMV). A regeneration system using immature male flowers as the explant was established. The frequency of somatic embryogenesis in male flower explants was influenced by the season in which the inflorescences were harvested. Further, the media requirements of various banana cultivars in respect to the 2,4-D concentration in the initiation media also differed. Following the optimisation of these and other parameters, embryogenic cell suspensions of several banana (Musa spp.) cultivars including 'Grand Nain' (AAA), 'Williams' (AAA), 'SH-3362' (AA), 'Goldfinger' (AAAB) and 'Bluggoe' (ABB) were successfully generated. Highly efficient transformation methods were developed for both 'Bluggoe' and 'Grand Nain'; this is the first report of microprojectile bombardment transformation of the commercially important 'Grand Nain' cultivar. Following bombardment of embryogenic suspension cells, regeneration was monitored from single transfom1ed cells to whole plants using a reporter gene encoding the green fluorescent protein (gfp). Selection with kanamycin enabled the regeneration of a greater number of plants than with geneticin, while still preventing the regeneration of non-transformed plants. Southern hybridisation confirmed the neomycin phosphotransferase gene (npt II) was stably integrated into the banana genome and that multiple transgenic lines were derived from single bombardments. The activity, stability and tissue specificity of the cauliflower mosaic virus 358 (CaMV 35S) and maize polyubiquitin-1 (Ubi-1) promoters were examined. In stably transformed banana, the Ubi-1 promoter provided approximately six-fold higher p-glucuronidase (GUS) activity than the CaMV 35S promoter, and both promoters remained active in glasshouse grown plants for the six months they were observed. The intergenic regions ofBBTV DNA-I to -6 were isolated and fused to either the uidA (GUS) or gfjJ reporter genes to assess their promoter activities. BBTV promoter activity…

Subjects/Keywords: Banana bunchy top disease; Bananas Diseases and pests; Bananas Somatic embryogenesis; Viral genetics; banana; musa; transformation; microprojectile bombardment; embryogenic cell suspension; pathogen-derived resistance; banana bunchy top nanovirus; banana bract mosaic potyvirus; virus resistance genes; thesis; doctoral

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Becker, D. K. (1999). The transformation of banana with potential virus resistance genes. (Thesis). Queensland University of Technology. Retrieved from https://eprints.qut.edu.au/37023/

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Becker, Douglas Kenneth. “The transformation of banana with potential virus resistance genes.” 1999. Thesis, Queensland University of Technology. Accessed February 28, 2021. https://eprints.qut.edu.au/37023/.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Becker, Douglas Kenneth. “The transformation of banana with potential virus resistance genes.” 1999. Web. 28 Feb 2021.

Vancouver:

Becker DK. The transformation of banana with potential virus resistance genes. [Internet] [Thesis]. Queensland University of Technology; 1999. [cited 2021 Feb 28]. Available from: https://eprints.qut.edu.au/37023/.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Becker DK. The transformation of banana with potential virus resistance genes. [Thesis]. Queensland University of Technology; 1999. Available from: https://eprints.qut.edu.au/37023/

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Queensland University of Technology

10. Chowpongpang, Srimek. Development of genetically engineered resistance to Papaya ringspot potyvirus (PRSV) in Thailand.

Degree: 2002, Queensland University of Technology

URL: http://eprints.qut.edu.au/37130/

Subjects/Keywords: Papaya ringspot virus; Papaya Diseases and pests Thailand; papaya ringspot potyvirus; PRSV; pathogen-derived resistance; coat protein (CP); transgenic; gene silencing; inverted repeat; dsRNA; RNA-mediated resistamce; thesis; doctoral

Record Details Similar Records Cite « Share

❌

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Chowpongpang, S. (2002). Development of genetically engineered resistance to Papaya ringspot potyvirus (PRSV) in Thailand. (Thesis). Queensland University of Technology. Retrieved from http://eprints.qut.edu.au/37130/

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Chowpongpang, Srimek. “Development of genetically engineered resistance to Papaya ringspot potyvirus (PRSV) in Thailand.” 2002. Thesis, Queensland University of Technology. Accessed February 28, 2021. http://eprints.qut.edu.au/37130/.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Chowpongpang, Srimek. “Development of genetically engineered resistance to Papaya ringspot potyvirus (PRSV) in Thailand.” 2002. Web. 28 Feb 2021.

Vancouver:

Chowpongpang S. Development of genetically engineered resistance to Papaya ringspot potyvirus (PRSV) in Thailand. [Internet] [Thesis]. Queensland University of Technology; 2002. [cited 2021 Feb 28]. Available from: http://eprints.qut.edu.au/37130/.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Chowpongpang S. Development of genetically engineered resistance to Papaya ringspot potyvirus (PRSV) in Thailand. [Thesis]. Queensland University of Technology; 2002. Available from: http://eprints.qut.edu.au/37130/

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

		in
And / Or
		in
And / Or
		in
And / Or
		in

Open Access Theses and Dissertations