subject:(paratuberculosis)

Oregon State University

1. Everman, Jamie L. Disease models and infectious phenotypes of Mycobacterium avium subspecies paratuberculosis.

Degree: PhD, Microbiology, 2014, Oregon State University

URL: http://hdl.handle.net/1957/54848

► Mycobacterium avium subspecies paratuberculosis (MAP) is the causative agent of Johne's disease, a chronic inflammatory bowel disease that affects ruminant populations worldwide. The characteristic stages… (more)

▼ Mycobacterium avium subspecies paratuberculosis (MAP) is the causative agent of Johne's disease, a chronic inflammatory bowel disease that affects ruminant populations worldwide. The characteristic stages of the disease make diagnosis difficult, resulting in silent transmission among animals in a herd for years before proper detection of the infection. The extensive prevalence of Johne's disease has driven a continuous effort to more readily understand the pathogenesis of the bacterium and to develop more effective preventative measures to curb the spread of the disease within herds. In this dissertation, we aim to create a more effective model for studying MAP infection within the intestinal mucosa, to utilize the milk-induced virulence phenotype to study how opsonization affects cell infection, and to study the metabolic interaction between MAP and the host phagocyte during infection. We describe a novel in vitro cell culture passage model which indicates that MAP changes during passage between bovine epithelial cells and macrophages, developing a more pro-inflammatory phenotype. We show that the inflammatory MAP phenotype not only increases gene expression of lipid metabolism- and modification-related genes, but that it is also composed of a set of lipids that are unique to the phenotype. Ultimately, we were able to identify these inflammatory-related transcripts in naturally MAP-infected bovine tissues, thus validating our model and indicating that the changing MAP phenotype may be a contributing factor in driving the development of inflammation within MAP infected animals. By using a different infectious phenotype that develops after MAP exposure to milk, a reservoir and transmission source of the bacterium, we demonstrate that opsonization of MAP results in more efficient translocation across an epithelial monolayer. Upon infection, we determine that macrophages more readily kill opsonized MAP in a rapid and specific manner. Furthermore, we begin to characterize one of the highest upregulated genes in this milk-induced phenotype, MAP1203, and its interaction with the intestinal epithelium. We establish that the putative cell wall associated protein is involved in both binding to and invasion of bovine MDBK epithelial cells when over-expressed in MAP during infection. Together, these data indicate the importance of the infectious phenotype developed after milk exposure and its role in the pathogenesis and transmission of Johne's disease. Finally, we utilize Acanthamoeba castellanii (amoeba) as a phagocytic host and describe the influence that MAP infection has on the metabolic activity of the cell. We detail how MAP stimulates the metabolism of the amoeba and how that stimulation directly mirrors the pattern of survival and the intracellular burden of MAP over the course of infection. We identify bacterial mutants that result in excessive or deficient stimulation of the metabolic activity within host cell and by utilizing phenotype arrays, we illustrate that amoeba change the use of specific… Advisors/Committee Members: Bermudez, Luiz E. (advisor), Rockey, Dan (committee member).

Subjects/Keywords: paratuberculosis; Mycobacterium avium paratuberculosis

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APA (6^th Edition):

Everman, J. L. (2014). Disease models and infectious phenotypes of Mycobacterium avium subspecies paratuberculosis. (Doctoral Dissertation). Oregon State University. Retrieved from http://hdl.handle.net/1957/54848

Chicago Manual of Style (16^th Edition):

Everman, Jamie L. “Disease models and infectious phenotypes of Mycobacterium avium subspecies paratuberculosis.” 2014. Doctoral Dissertation, Oregon State University. Accessed January 21, 2021. http://hdl.handle.net/1957/54848.

MLA Handbook (7^th Edition):

Everman, Jamie L. “Disease models and infectious phenotypes of Mycobacterium avium subspecies paratuberculosis.” 2014. Web. 21 Jan 2021.

Vancouver:

Everman JL. Disease models and infectious phenotypes of Mycobacterium avium subspecies paratuberculosis. [Internet] [Doctoral dissertation]. Oregon State University; 2014. [cited 2021 Jan 21]. Available from: http://hdl.handle.net/1957/54848.

Council of Science Editors:

Everman JL. Disease models and infectious phenotypes of Mycobacterium avium subspecies paratuberculosis. [Doctoral Dissertation]. Oregon State University; 2014. Available from: http://hdl.handle.net/1957/54848

Cornell University

2. Mitchell, Rebecca. Transmission Dynamics Of Mycobacterium Avium Subspecies Paratuberculosis In Closed Dairy Herds In The Us.

Degree: PhD, Veterinary Medicine, 2011, Cornell University

URL: http://hdl.handle.net/1813/29474

► In this work we present work focused on within farm dynamics of Mycobacterium avium subsp paratuberculosis (MAP) infection on commercial US dairies. We focused on… (more)

Subjects/Keywords: transmission dynamics; paratuberculosis; mathematical models

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APA (6^th Edition):

Mitchell, R. (2011). Transmission Dynamics Of Mycobacterium Avium Subspecies Paratuberculosis In Closed Dairy Herds In The Us. (Doctoral Dissertation). Cornell University. Retrieved from http://hdl.handle.net/1813/29474

Chicago Manual of Style (16^th Edition):

Mitchell, Rebecca. “Transmission Dynamics Of Mycobacterium Avium Subspecies Paratuberculosis In Closed Dairy Herds In The Us.” 2011. Doctoral Dissertation, Cornell University. Accessed January 21, 2021. http://hdl.handle.net/1813/29474.

MLA Handbook (7^th Edition):

Mitchell, Rebecca. “Transmission Dynamics Of Mycobacterium Avium Subspecies Paratuberculosis In Closed Dairy Herds In The Us.” 2011. Web. 21 Jan 2021.

Vancouver:

Mitchell R. Transmission Dynamics Of Mycobacterium Avium Subspecies Paratuberculosis In Closed Dairy Herds In The Us. [Internet] [Doctoral dissertation]. Cornell University; 2011. [cited 2021 Jan 21]. Available from: http://hdl.handle.net/1813/29474.

Council of Science Editors:

Mitchell R. Transmission Dynamics Of Mycobacterium Avium Subspecies Paratuberculosis In Closed Dairy Herds In The Us. [Doctoral Dissertation]. Cornell University; 2011. Available from: http://hdl.handle.net/1813/29474

3. Gilardoni, Liliana Rosa. Estrategia diagnóstica de paratuberculosis en bovinos.

Degree: Ciencias Veterinarias, 2012, Universidad de Buenos Aires

URL: http://repositoriouba.sisbi.uba.ar/gsdl/cgi-bin/library.cgi?a=d&c=posgrauba&cl=CL1&d=HWA_1558 ; http://repositoriouba.sisbi.uba.ar/gsdl/collect/posgrauba/index/assoc/HWA_1558.dir/1558.PDF

► Paratuberculosis (PTB) is a chronic disease of ruminants, characterized by a long incubation period and development of granulomatous enteritis and diarrhea in advanced stages of… (more)

▼ Paratuberculosis (PTB) is a chronic disease of ruminants, characterized by a long incubation period and development of granulomatous enteritis and diarrhea in advanced stages of the disease. It is caused by Mycobacterium avium\nsubspecies paratuberculosis (Map). Its economic relevance is due to reduced\nmilk yield, progressive cachexia and mortality.\nDepending on the severity of clinical signs, the evolution of bovine PTB can be\ndivided into four stages: silent, subclinical, clinical, and advanced. The animals\nin the early stages, which are usually the longest, excrete Map by colostrum,\nmilk and feces, with no evidence of clinical signs. In this way, the spread of the\ninfection to other susceptible animals, particularly calves, takes place. For these reasons, control of the disease should be based on early detection\nand rapid culling of infected animals. However, currently available diagnostic\ntests have not enough sensitivity to do feasible the implementation of this type\nof strategy. The recognized and currently in use diagnostic tests for the diagnosis of PTB\nare fecal culture and indirect ELISA test for the detection of serum antibodies. In\nsubclinical stages the sensitivity of these tests is below 50%. In order to improve the diagnostic sensitivity, biotechnology was applied for the\ndevelopment of new methods, as the polymerase chain reaction (PCR) that can be applied in feces, milk and tissues samples.\nHowever, the elimination of Map by feces or milk is intermittent and with a low\nconcentration. Therefore to increase sensitivity a method of concentration of\nMap was developed: immunomagnetic separation (IMS) using magnetic\nnanoparticles coupled to polyclonal antibodies antiMap. The procedure applied to bovine milk not only increases the concentration of Map, but also removes\nthe milk components PCR inhibitors. The purpose of this thesis was to develop a procedure to identify Map in milk samples. The developed protocol has two stages: a. concentration of microorganisms by immunomagnetic separation with magnetic nanoparticles (immunomagnetic beads) coupled to specific\nmonoclonal and polyclonal antibodies produced in the Faculty of Veterinary Science, University of Buenos Aires.\nb. identification of genetic material by the application of IS900 PCR with\nprimers designed in the same Faculty.\nIn addition, in order to evaluate the performance of the method in field\nconditions (preliminary assessment), samples from dairy cows from both\ninfected and free farms were analyzed. The maximum adhesion of immunomagnetic beads was obtained when beads\ncoupled to monoclonal antibodies were used simultaneously with beads\ncoupled to polyclonal antibodies (107 CFU/mL).\nA greater analytical sensitivity was obtained with the IS900 PCR with primers\ndesigned by Dr. S. L, Mundo, at the Faculty of Veterinary Science, compared to\nthe IS900 PCR with primers designed by Dr. Collins et al. and ISF57 PCR (the\nmethods were able to detect 101, 103 and 102 Map CFU/mL respectively).\nFurthermore, the IS900 PCR did not… Advisors/Committee Members: Paolicci, Fernando, Mundo, Silvia Leonor.

Subjects/Keywords: Bovinos; Paratuberculosis; Diagnóstico; Microbiología

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APA (6^th Edition):

Gilardoni, L. R. (2012). Estrategia diagnóstica de paratuberculosis en bovinos. (Thesis). Universidad de Buenos Aires. Retrieved from http://repositoriouba.sisbi.uba.ar/gsdl/cgi-bin/library.cgi?a=d&c=posgrauba&cl=CL1&d=HWA_1558 ; http://repositoriouba.sisbi.uba.ar/gsdl/collect/posgrauba/index/assoc/HWA_1558.dir/1558.PDF

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Gilardoni, Liliana Rosa. “Estrategia diagnóstica de paratuberculosis en bovinos.” 2012. Thesis, Universidad de Buenos Aires. Accessed January 21, 2021. http://repositoriouba.sisbi.uba.ar/gsdl/cgi-bin/library.cgi?a=d&c=posgrauba&cl=CL1&d=HWA_1558 ; http://repositoriouba.sisbi.uba.ar/gsdl/collect/posgrauba/index/assoc/HWA_1558.dir/1558.PDF.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Gilardoni, Liliana Rosa. “Estrategia diagnóstica de paratuberculosis en bovinos.” 2012. Web. 21 Jan 2021.

Vancouver:

Gilardoni LR. Estrategia diagnóstica de paratuberculosis en bovinos. [Internet] [Thesis]. Universidad de Buenos Aires; 2012. [cited 2021 Jan 21]. Available from: http://repositoriouba.sisbi.uba.ar/gsdl/cgi-bin/library.cgi?a=d&c=posgrauba&cl=CL1&d=HWA_1558 ; http://repositoriouba.sisbi.uba.ar/gsdl/collect/posgrauba/index/assoc/HWA_1558.dir/1558.PDF.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Gilardoni LR. Estrategia diagnóstica de paratuberculosis en bovinos. [Thesis]. Universidad de Buenos Aires; 2012. Available from: http://repositoriouba.sisbi.uba.ar/gsdl/cgi-bin/library.cgi?a=d&c=posgrauba&cl=CL1&d=HWA_1558 ; http://repositoriouba.sisbi.uba.ar/gsdl/collect/posgrauba/index/assoc/HWA_1558.dir/1558.PDF

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Edinburgh

4. Nicol, Louise Maureen Marie. Investigating differential T cell polarization in the two pathological forms of sheep paratuberculosis.

Degree: PhD, 2016, University of Edinburgh

URL: http://hdl.handle.net/1842/22855

► Paratuberculosis is a chronic enteropathy of ruminants that presents as two distinct disease forms in sheep; paucibacillary (or tuberculoid) and multibacillary (or lepromatous) disease. The… (more)

▼ Paratuberculosis is a chronic enteropathy of ruminants that presents as two distinct disease forms in sheep; paucibacillary (or tuberculoid) and multibacillary (or lepromatous) disease. The immunopathology of paucibacillary and multibacillary sheep paratuberculosis has been linked to inflammatory Th1/Th17 cell and Th2/macrophage responses respectively. IL23 and IL25 are key to the development of these responses by interaction with their complex receptors, IL23R/IL12RB1 and IL17RA/IL17RB. Furthermore, the polarization of T cells and the development of appropriate immune responses is controlled by the master regulator transcription factor; T-bet, GATA3, RORγt and RORα. In humans, variations in the structure, sequence and/or expression of the genes encoding these proteins have been implicated in the different pathological forms of tuberculosis and leprosy, and gastrointestinal inflammatory disorders such as Crohn’s disease. In the current study, sequencing has identified multiple transcript variants of sheep IL23R, IL12RB1 and IL17RB and a single IL17RA transcript. RT-qPCR assays were developed for the cytokine receptor variants identified in this study and known transcript variants of the transcription factor genes. Expression levels were compared in the ileo cecal lymph node of paucibacillary or multibacillary paratuberculosis diseased sheep. Of the cytokine receptors; the IL12RB1v3 variant, which lacks the receptor activation motif, was differentially expressed and was significantly increased in multibacillary disease; this may contribute to high Th2 responses. Full length IL17RB was differentially expressed and was significantly increased in multibacillary pathology, which may also contribute to Th2 polarization. IL17RA was significantly increased in paucibacillary disease. The contrast between the IL17RA and IL17RB results may indicate that, in addition to Th1 cells, Th17 T cells are also involved in paucibacillary pathology. Of the transcription factor transcripts; full length TBX21 (T-bet) was differentially expressed and was significantly increased in paucibacillary disease; this may explain increased Th1 responses in these sheep. Full length GATA3 was significantly increased in paucibacillary compared to multibacillary sheep, suggesting a loss of Th2 responses in late-stage multibacillary pathology. RORAv1 variant was differentially expressed and was significantly increased in paucibacillary pathology, indicating a role of Th17 T cells in paucibacillary pathology.

Subjects/Keywords: 636.3; paratuberculosis; IL23; IL25; sheep

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APA (6^th Edition):

Nicol, L. M. M. (2016). Investigating differential T cell polarization in the two pathological forms of sheep paratuberculosis. (Doctoral Dissertation). University of Edinburgh. Retrieved from http://hdl.handle.net/1842/22855

Chicago Manual of Style (16^th Edition):

Nicol, Louise Maureen Marie. “Investigating differential T cell polarization in the two pathological forms of sheep paratuberculosis.” 2016. Doctoral Dissertation, University of Edinburgh. Accessed January 21, 2021. http://hdl.handle.net/1842/22855.

MLA Handbook (7^th Edition):

Nicol, Louise Maureen Marie. “Investigating differential T cell polarization in the two pathological forms of sheep paratuberculosis.” 2016. Web. 21 Jan 2021.

Vancouver:

Nicol LMM. Investigating differential T cell polarization in the two pathological forms of sheep paratuberculosis. [Internet] [Doctoral dissertation]. University of Edinburgh; 2016. [cited 2021 Jan 21]. Available from: http://hdl.handle.net/1842/22855.

Council of Science Editors:

Nicol LMM. Investigating differential T cell polarization in the two pathological forms of sheep paratuberculosis. [Doctoral Dissertation]. University of Edinburgh; 2016. Available from: http://hdl.handle.net/1842/22855

Iowa State University

5. Merkal, Richard Sterling. Histochemical, serological, allergic, and cultural studies of experimental paratuberculosis in sheep.

Degree: 1965, Iowa State University

URL: https://lib.dr.iastate.edu/rtd/3368

Subjects/Keywords: Mycobacterium paratuberculosis; Paratuberculosis; Microbiology

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APA (6^th Edition):

Merkal, R. S. (1965). Histochemical, serological, allergic, and cultural studies of experimental paratuberculosis in sheep. (Thesis). Iowa State University. Retrieved from https://lib.dr.iastate.edu/rtd/3368

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Merkal, Richard Sterling. “Histochemical, serological, allergic, and cultural studies of experimental paratuberculosis in sheep.” 1965. Thesis, Iowa State University. Accessed January 21, 2021. https://lib.dr.iastate.edu/rtd/3368.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Merkal, Richard Sterling. “Histochemical, serological, allergic, and cultural studies of experimental paratuberculosis in sheep.” 1965. Web. 21 Jan 2021.

Vancouver:

Merkal RS. Histochemical, serological, allergic, and cultural studies of experimental paratuberculosis in sheep. [Internet] [Thesis]. Iowa State University; 1965. [cited 2021 Jan 21]. Available from: https://lib.dr.iastate.edu/rtd/3368.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Merkal RS. Histochemical, serological, allergic, and cultural studies of experimental paratuberculosis in sheep. [Thesis]. Iowa State University; 1965. Available from: https://lib.dr.iastate.edu/rtd/3368

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Michigan State University

6. DeKuiper, Justin Lee. Inflammatory TH17 responses to infection with Mycobacterium avium subspecies paratuberculosis (MAP) in cattle and their potential role in the development of Johne's disease.

Degree: 2019, Michigan State University

URL: http://etd.lib.msu.edu/islandora/object/etd:48303

►

Thesis Ph. D. Michigan State University. Animal Science 2019.

Mycobacterium avium subspecies paratuberculosis (MAP) causes a chronic inflammatory gastrointestinal disease of ruminants known as Johne’s… (more)

▼

Thesis Ph. D. Michigan State University. Animal Science 2019.

Mycobacterium avium subspecies paratuberculosis (MAP) causes a chronic inflammatory gastrointestinal disease of ruminants known as Johne’s disease (JD). MAP primarily colonizes the ileum of ruminants, leading to reduced nutrient absorption, chronic diarrhea, and eventually death. The percentage of dairy operations infected MAP in the US may have risen to as much as 91% from earlier reports of 68%. In correspondence, an estimated dairy industry loss has increased from 200 million to 1.5 billion was due to JD. Control of JD is difficult largely due to insensitive diagnostic tools, a long subclinical stage of infection, and lack of effective vaccines. Correlates of protection are lacking in model systems of JD and the sources of inflammation due to JD are not well characterized. Inside macrophages, MAP survives and replicates. Monocyte-derived macrophages (MDMs), peripheral blood mononuclear cells (PBMCs), and various T-cells interact with MAP. Commonly studied immune responses, such as the Th1/Th2 paradigm, do not adequately explain host responses to MAP. The major remaining knowledge gaps in MAP immunopathogenesis include key inflammatory responders in the ileum, and how naïve T-cell responsive choice (Th1, Th2, Th17) is influenced. A potential role for non-classical immune responses to MAP, such as that mediated by Th17 cells, has been suggested. Indeed, MAP antigens induce mRNAs encoding the cytokines IL-23 and IL-17A in bovine PBMCs. IL-23 and IL-17A production are both associated with Th17-like immune responses. Th17 cells are also defined by surface expression of the IL-23 receptor (IL-23R). The mean relative percent (MRP) of T cell subtypes expressing IL-23R was determined by flow cytometry and indicated an increase in mean relative percent (MRP) of T cells (CD4+, CD8+, and TCR1+) in peripheral blood mononuclear cells (PBMCs) of JD+ cows with high and low expression of IL-23R (IL-23RHigh and IL-23RLow) when compared to JD- cows. Although MAP stimulates PBMCs to secrete IL-17A in-vitro, there were no differences in IL-17A levels between subclinical JD+ and JD- cows when analyzed by ELISA. Plasma with low JD+ score values had significantly more IL-17A when compared to plasma with high JD+ score values, establishing a moderate correlation between JD+ score and IL-17A. However, overall plasma from JD+ cows had significantly less IL-17A than plasma from JD- cows. Unlike IL-17A, IL-23 was greater in plasma from JD+ cows than in JD- cows. Evaluating the relationship that MAP is having on APCs and CD3+ in relation to Th17 cytokines (IL-23, IL-17A, IL-17F, IL-22, IL-27) as well as Th1 cytokine IFNγ, CD3+ were stimulated with MAP in the presence of APCs (MDMs or B cells) or alone and evaluated by RT-qPCR. MAP stimulation significantly increased production of mRNA encoding Th17 cytokines and IFNγ in CD3+ T cells regardless of APCs. However, the presence of MDMs significantly increase the quantity of mRNA. Lastly, we observed that αβ T cells (mostly CD4+)…

Advisors/Committee Members: Coussens, Paul M, Steibel, Juan, Petroff, Peggy, Norby, Bo.

Subjects/Keywords: Mycobacterium avium paratuberculosis; Paratuberculosis; Cattle – Diseases; Immunology; Animal diseases

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

DeKuiper, J. L. (2019). Inflammatory TH17 responses to infection with Mycobacterium avium subspecies paratuberculosis (MAP) in cattle and their potential role in the development of Johne's disease. (Thesis). Michigan State University. Retrieved from http://etd.lib.msu.edu/islandora/object/etd:48303

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

DeKuiper, Justin Lee. “Inflammatory TH17 responses to infection with Mycobacterium avium subspecies paratuberculosis (MAP) in cattle and their potential role in the development of Johne's disease.” 2019. Thesis, Michigan State University. Accessed January 21, 2021. http://etd.lib.msu.edu/islandora/object/etd:48303.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

DeKuiper, Justin Lee. “Inflammatory TH17 responses to infection with Mycobacterium avium subspecies paratuberculosis (MAP) in cattle and their potential role in the development of Johne's disease.” 2019. Web. 21 Jan 2021.

Vancouver:

DeKuiper JL. Inflammatory TH17 responses to infection with Mycobacterium avium subspecies paratuberculosis (MAP) in cattle and their potential role in the development of Johne's disease. [Internet] [Thesis]. Michigan State University; 2019. [cited 2021 Jan 21]. Available from: http://etd.lib.msu.edu/islandora/object/etd:48303.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

DeKuiper JL. Inflammatory TH17 responses to infection with Mycobacterium avium subspecies paratuberculosis (MAP) in cattle and their potential role in the development of Johne's disease. [Thesis]. Michigan State University; 2019. Available from: http://etd.lib.msu.edu/islandora/object/etd:48303

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Massey University

7. Sitthirit, Panan. The development of polyester bead-based particulate subunit vaccine against Johne's disease : a thesis presented in partial fulfillment of the requirements of the degree of Master of Science in Microbiology, Massey University, Palmerston North, Institute of Fundamental Science .

Degree: 2015, Massey University

URL: http://hdl.handle.net/10179/11363

► Johne’s disease is the intestinal infection in ruminants caused by Mycobacterium avium subspecies paratuberculosis (MAP). The disease is economically important in the dairy industry as… (more)

Subjects/Keywords: Escherichia coli; Biotechnology; Paratuberculosis; Johne's disease; Veterinary vaccines; Mycobacterium avium paratuberculosis

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APA (6^th Edition):

Sitthirit, P. (2015). The development of polyester bead-based particulate subunit vaccine against Johne's disease : a thesis presented in partial fulfillment of the requirements of the degree of Master of Science in Microbiology, Massey University, Palmerston North, Institute of Fundamental Science . (Thesis). Massey University. Retrieved from http://hdl.handle.net/10179/11363

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Sitthirit, Panan. “The development of polyester bead-based particulate subunit vaccine against Johne's disease : a thesis presented in partial fulfillment of the requirements of the degree of Master of Science in Microbiology, Massey University, Palmerston North, Institute of Fundamental Science .” 2015. Thesis, Massey University. Accessed January 21, 2021. http://hdl.handle.net/10179/11363.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Sitthirit, Panan. “The development of polyester bead-based particulate subunit vaccine against Johne's disease : a thesis presented in partial fulfillment of the requirements of the degree of Master of Science in Microbiology, Massey University, Palmerston North, Institute of Fundamental Science .” 2015. Web. 21 Jan 2021.

Vancouver:

Sitthirit P. The development of polyester bead-based particulate subunit vaccine against Johne's disease : a thesis presented in partial fulfillment of the requirements of the degree of Master of Science in Microbiology, Massey University, Palmerston North, Institute of Fundamental Science . [Internet] [Thesis]. Massey University; 2015. [cited 2021 Jan 21]. Available from: http://hdl.handle.net/10179/11363.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Sitthirit P. The development of polyester bead-based particulate subunit vaccine against Johne's disease : a thesis presented in partial fulfillment of the requirements of the degree of Master of Science in Microbiology, Massey University, Palmerston North, Institute of Fundamental Science . [Thesis]. Massey University; 2015. Available from: http://hdl.handle.net/10179/11363

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Massey University

8. Gautam, Milan. Epidemiology of ovine paratuberculosis in New Zealand.

Degree: PhD, 2019, Massey University

URL: http://hdl.handle.net/10179/15721

► The overall goal of this PhD project was to better understand the general epidemiology of ovine paratuberculosis (PTb) and the speciﬁc molecular characteristics of the… (more)

▼ The overall goal of this PhD project was to better understand the general epidemiology of ovine paratuberculosis (PTb) and the speciﬁc molecular characteristics of the causing organism Mycobacterium avium subspecies paratuberculosis in New Zealand. To begin with, current control measures for clinical PTb in New Zealand’s major pastoral livestock species (dairy cattle, beef cattle, sheep, deer) were reviewed. Infection with Map is common in all these species and control is voluntary for all livestock industries. Control measures aim to reduce the incidence rate of clinical PTb rather than to eradicate Map infection. Dairy and deer industries have developed resources describing best-practice management options that assist farmers and veterinarians to advise their clients about speciﬁc control plans. There is no national control programme for sheep and beef cattle. However, unlike for cattle and deer, the use of a commercial vaccine is licensed for sheep. Evidence in this thesis suggests that vaccination may be a cost effective option for ﬂocks that experience a high incidence of clinical disease. For deer, there is a national abattoir surveillance programme that aims to alert farmers of unusually high rates of PTb-like lesions in deer at slaughter. Evaluations of the biological and economic effectiveness of voluntary control still remains to be undertaken for all industries. Work in this thesis estimated the on-farm economic cost of clinical PTb in sheep (ovine Johne’s disease, OJD) in New Zealand. It was based on data about the incidence of clinical PTb and overall mortality from 20 OJD-affected farms. The beneﬁt-cost ratio of vaccination was estimated. Farms were categorized as either ﬁne-wool breed (Merino, Half-bred, Corriedale) or other breeds and calculations were stratiﬁed for these two farm categories. The estimated mortality due to OJD was 2.7 times as high in ﬁne-wool as in other breeds with large variation between farms. A stochastic simulation for a hypothetical ﬂock with 2,000 breeding ewes resulted in an average annual cost of OJD-mortality of NZ 13,100 in ﬁne-wool and NZ 4,300 in other breeds. Vaccinating replacement lambs against OJD would be cost-effective in most ﬂocks when the pre-vaccination annual OJD ewe mortality was >1%. Accurate on-farm observation of OJD to establish incidence would help farmers to make better decisions about vaccination. Frozen-stored faecal and serum samples of individual sheep with no signs of clinical disease from 45 commercial ﬂocks from a 2013 study that determined pooled faecal culture (PFC) status were used to determine faecal Map shedding and antibody in serum. A total of 878 faecal samples were tested with direct faecal real-time quantitative PCR (qPCR) to determine Map shedding prevalence and abundance in individual animals. In addition, the qPCR results were compared with Map antibody ELISA results from 837 corresponding sera to correlate the observed shedding prevalence with sero-prevalence. Overall, 13.1% of faecal samples…

Subjects/Keywords: Paratuberculosis; New Zealand; Epidemiology; Mycobacterium avium paratuberculosis; Sheep; Diseases

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Gautam, M. (2019). Epidemiology of ovine paratuberculosis in New Zealand. (Doctoral Dissertation). Massey University. Retrieved from http://hdl.handle.net/10179/15721

Chicago Manual of Style (16^th Edition):

Gautam, Milan. “Epidemiology of ovine paratuberculosis in New Zealand.” 2019. Doctoral Dissertation, Massey University. Accessed January 21, 2021. http://hdl.handle.net/10179/15721.

MLA Handbook (7^th Edition):

Gautam, Milan. “Epidemiology of ovine paratuberculosis in New Zealand.” 2019. Web. 21 Jan 2021.

Vancouver:

Gautam M. Epidemiology of ovine paratuberculosis in New Zealand. [Internet] [Doctoral dissertation]. Massey University; 2019. [cited 2021 Jan 21]. Available from: http://hdl.handle.net/10179/15721.

Council of Science Editors:

Gautam M. Epidemiology of ovine paratuberculosis in New Zealand. [Doctoral Dissertation]. Massey University; 2019. Available from: http://hdl.handle.net/10179/15721

Cornell University

9. Smith, Rebecca. Statistical Modeling Of Longitudinal Data From Dairy Herds: Mycobacterium Avium Subsp. Paratuberculosis.

Degree: PhD, Veterinary Medicine, 2011, Cornell University

URL: http://hdl.handle.net/1813/30713

► Mycobacterium avium subsp. paratuberculosis (MAP) causes infections in ruminants characterized by long latent periods, imperfect diagnostic tests, and production effects sufficient to result in early… (more)

▼ Mycobacterium avium subsp. paratuberculosis (MAP) causes infections in ruminants characterized by long latent periods, imperfect diagnostic tests, and production effects sufficient to result in early culling. In order to optimize control of this pathogen, the exact effects of both the infection and the control strategies must be understood. The goal of this dissertation is to statistically analyze various aspects of MAP in dairy herds, including the production effects of infection, its distribution in the dairy herd environment, and the transmission rates of animals shedding MAP. For this purpose, longitudinal data sets from commercial dairy herds have been analyzed, allowing for a more thorough understanding of MAP in typical farms. Cows in the high-shedding category (>30 cfu/g of MAP in feces) were found to produce approximately 4 kg less milk per day, on average, and to have higher culling rates and lower calving rates than non-shedding cows. In addition, the number of high-shedding animals in a pen was positively correlated with the amount of MAP cultured from the environment in that pen. In contrast, low-shedding cows were found to have higher culling rates than nonshedding cows, but no significant difference in calving rates or milk production. The average amount of fecal shedding in the herd was found to be predictive of both the odds of MAP being found in environmental samples and the amount of MAP in those samples, but environmental sampling was not found to be a sensitive herd-level diagnostic test. These results will enable optimization of economic models for MAP control by providing quantitative estimates of the effect of MAP on commercial dairy farms. In addition, it was found that reversible-jump Markov Chain Monte Carlo models are unable to estimate transmission rates for MAP using current longitudinal data sets, due to the large amount of missing data. Advisors/Committee Members: Grohn, Yrjo Tapio (chair), Tauer, Loren William (committee member), Strawderman, Robert Lee (committee member), Schukken, Ynte Hein (committee member).

Subjects/Keywords: statistical modeling; Mycobacterium avium paratuberculosis; longitudinal data

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Smith, R. (2011). Statistical Modeling Of Longitudinal Data From Dairy Herds: Mycobacterium Avium Subsp. Paratuberculosis. (Doctoral Dissertation). Cornell University. Retrieved from http://hdl.handle.net/1813/30713

Chicago Manual of Style (16^th Edition):

Smith, Rebecca. “Statistical Modeling Of Longitudinal Data From Dairy Herds: Mycobacterium Avium Subsp. Paratuberculosis.” 2011. Doctoral Dissertation, Cornell University. Accessed January 21, 2021. http://hdl.handle.net/1813/30713.

MLA Handbook (7^th Edition):

Smith, Rebecca. “Statistical Modeling Of Longitudinal Data From Dairy Herds: Mycobacterium Avium Subsp. Paratuberculosis.” 2011. Web. 21 Jan 2021.

Vancouver:

Smith R. Statistical Modeling Of Longitudinal Data From Dairy Herds: Mycobacterium Avium Subsp. Paratuberculosis. [Internet] [Doctoral dissertation]. Cornell University; 2011. [cited 2021 Jan 21]. Available from: http://hdl.handle.net/1813/30713.

Council of Science Editors:

Smith R. Statistical Modeling Of Longitudinal Data From Dairy Herds: Mycobacterium Avium Subsp. Paratuberculosis. [Doctoral Dissertation]. Cornell University; 2011. Available from: http://hdl.handle.net/1813/30713

Rhodes University

10. Clarke, Matthew David. Biochemical characterization of the β-mannanase activity of Bacillus paralicheniformis SVD1.

Degree: Faculty of Science, Biochemistry and Microbiology, 2019, Rhodes University

URL: http://hdl.handle.net/10962/67570

► Products produced via the enzymatic hydrolysis of lignocellulosic biomass, the most abundant renewable terrestrial source of carbon, can potentially replace a lot of the fuels… (more)

▼ Products produced via the enzymatic hydrolysis of lignocellulosic biomass, the most abundant renewable terrestrial source of carbon, can potentially replace a lot of the fuels and chemicals currently produced using non-renewable hydrocarbons. Mannan is a polysaccharide component of lignocellulose that is abundant in softwoods and legume seeds. Enzymatic hydrolysis of mannan by β-mannanases has various industrial applications, including use in biofuel and prebiotic mannooligosaccharide (MOS) production for the improvement of human and animal health. The industrial use of β-mannanases depends on their biochemical characteristics, such as their activity, stability and substrate specificity. Knowledge of their synergistic interactions with other enzymes is also useful for effective hydrolysis. Bacillus paralicheniformis SVD1 was used as a source for β-mannanases. The two mannanases of B. paralicheniformis SVD1 have not been biochemically characterized apart from minor characterization of crude β-mannanase activity. The protein sequences of the two β-mannanases, of glycosyl hydrolase family 5 and 26, have a 95% - 96% identity to the β-mannanases of B. licheniformis DSM13T (=ATCC14580T). These small protein sequence differences could lead to quite different biochemical characteristics. These mannanases were characterized as these enzymes may have industrially useful characteristics. To induce mannanase production, B. paralicheniformis SVD1 was cultured in broth containing the mannan substrate locust bean gum. Various growth curve parameters were measured over 72 h. Mannanase activity was the highest after 48 h of growth - this was the time at which mannanase activity was concentrated, using 3 kDa centrifugal filtration devices, for biochemical characterization of the crude activity. Zymography revealed that the crude concentrated mannanase fraction consisted of at least two mannanases with relative molecular weights (MWs) of 29.6 kDa and 33 kDa. This was smaller than expected – based on their theoretical molecular masses. Protease activity, which was detected in the broth, was probably the reason. There were two pH optima, pH 5.0 and pH 7.0, which also indicated the presence of two mannanases. The concentrated mannanase displayed characteristics that were expected of a B. paralicheniformis β-mannanase. The temperature optimum was 50°C and the activity loss was less than 7% at 50°C after 24 h. Substrate specificity assays revealed that there was predominantly mannanase activity present. Thin layer chromatography (TLC) analysis of mannan and MOS hydrolysis showed that mainly M2 and M3 MOS were produced; only MOS with a degree of polymerization of 4 or higher were hydrolyzed. Hydrolysis was minimal on mannoligosaccharides with galactose substituents. Activity and MOS production was the highest on soluble, low branched mannan substrates. The highest activity observed was on konjac glucomannan. Purification of the mannanase activity was then attempted using various methods. Ammonium sulfate precipitation, acetone precipitation,…

Subjects/Keywords: Mycobacterium avium paratuberculosis; Enzymes – Biotechnology; Lignocellulose – Biotechnology

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APA (6^th Edition):

Clarke, M. D. (2019). Biochemical characterization of the β-mannanase activity of Bacillus paralicheniformis SVD1. (Thesis). Rhodes University. Retrieved from http://hdl.handle.net/10962/67570

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Clarke, Matthew David. “Biochemical characterization of the β-mannanase activity of Bacillus paralicheniformis SVD1.” 2019. Thesis, Rhodes University. Accessed January 21, 2021. http://hdl.handle.net/10962/67570.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Clarke, Matthew David. “Biochemical characterization of the β-mannanase activity of Bacillus paralicheniformis SVD1.” 2019. Web. 21 Jan 2021.

Vancouver:

Clarke MD. Biochemical characterization of the β-mannanase activity of Bacillus paralicheniformis SVD1. [Internet] [Thesis]. Rhodes University; 2019. [cited 2021 Jan 21]. Available from: http://hdl.handle.net/10962/67570.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Clarke MD. Biochemical characterization of the β-mannanase activity of Bacillus paralicheniformis SVD1. [Thesis]. Rhodes University; 2019. Available from: http://hdl.handle.net/10962/67570

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Guelph

11. Mead, Philip James. Infection of monocyte-derived macrophages with a reporter Map strain: Validation of the susceptibilty SNP (-298A/G) in the bovine MIF gene.

Degree: MS, Department of Animal and Poultry Science, 2013, University of Guelph

URL: https://atrium.lib.uoguelph.ca/xmlui/handle/10214/7503

► Johne’s disease (JD) is a chronic, infectious enteritis of ruminant animals caused by Mycobacterium avium subsp. paratuberculosis (Map). Monitoring host-pathogen interaction over time is critical… (more)

Subjects/Keywords: Johnes Disease; Mycobacterium avium subsp paratuberculosis

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APA (6^th Edition):

Mead, P. J. (2013). Infection of monocyte-derived macrophages with a reporter Map strain: Validation of the susceptibilty SNP (-298A/G) in the bovine MIF gene. (Masters Thesis). University of Guelph. Retrieved from https://atrium.lib.uoguelph.ca/xmlui/handle/10214/7503

Chicago Manual of Style (16^th Edition):

Mead, Philip James. “Infection of monocyte-derived macrophages with a reporter Map strain: Validation of the susceptibilty SNP (-298A/G) in the bovine MIF gene.” 2013. Masters Thesis, University of Guelph. Accessed January 21, 2021. https://atrium.lib.uoguelph.ca/xmlui/handle/10214/7503.

MLA Handbook (7^th Edition):

Mead, Philip James. “Infection of monocyte-derived macrophages with a reporter Map strain: Validation of the susceptibilty SNP (-298A/G) in the bovine MIF gene.” 2013. Web. 21 Jan 2021.

Vancouver:

Mead PJ. Infection of monocyte-derived macrophages with a reporter Map strain: Validation of the susceptibilty SNP (-298A/G) in the bovine MIF gene. [Internet] [Masters thesis]. University of Guelph; 2013. [cited 2021 Jan 21]. Available from: https://atrium.lib.uoguelph.ca/xmlui/handle/10214/7503.

Council of Science Editors:

Mead PJ. Infection of monocyte-derived macrophages with a reporter Map strain: Validation of the susceptibilty SNP (-298A/G) in the bovine MIF gene. [Masters Thesis]. University of Guelph; 2013. Available from: https://atrium.lib.uoguelph.ca/xmlui/handle/10214/7503

University of Otago

12. McCulloch, Timothy Ryan. Genetic Markers to Identify the Resilient and Susceptible Phenotypes to Johne’s Disease in Red Deer (Cervus elaphus) .

Degree: University of Otago

URL: http://hdl.handle.net/10523/4982

► Johne’s disease, a chronic granulomatous enteritis caused by infection of the pathogen Mycobacterium avium subspecies paratuberculosis, can be a major cause of production loses in… (more)

▼ Johne’s disease, a chronic granulomatous enteritis caused by infection of the pathogen Mycobacterium avium subspecies paratuberculosis, can be a major cause of production loses in farmed ruminants. At present, there are no viable options for treatment or prevention of the disease. With prevalence of Johne’s disease suspected to be on the rise, a method of control is required. Red deer stags with resilient and susceptible phenotypes to Johne’s disease have been identified previously by the DRL, and these phenotypes appear to have a high paternal heritability. The present study is a contribution towards the identification of biomarkers for either phenotype to produce a diagnostic test to proactively identify whether a naïve animal is likely to be resilient or susceptible to the disease. This diagnostic assay would be useful for selection of resilient animals for breeding, producing a genetically resilient herd to reduce Johne’s disease associated production losses. As susceptibility to Johne’s disease was thought to be due to a dysregulation of the innate immune system, this project investigated differential expression of genetic markers from macrophages of resilient and susceptible animals. Monocyte-derived macrophages were cultured from cervine blood and stimulated with viable Mycobacterium paratuberculosis. Quantitative-PCR was used to analyse changes in expression of target genes. Of the investigated gene targets, ISG15 and ISG20 showed particular promise, where animals of the resilient phenotype upregulated expression to a greater degree than animals of the susceptible phenotype. Moreover, IL1A, IL12A, MAPK8, NOS2, PKLR, and STAT3 showed sufficient differential expression between the phenotypes to suggest their potential for further investigation. This study also investigated the feasibility of PBMC culture as a platform for a diagnostic assay. This assay presented favorably in terms of volume of blood required and culture duration compared to the MDM assay. However, no consistent differential gene expression was observed from the investigated genes, and more work is required to identify functional genetic markers. Taken together, these results demonstrate the potential for using differential gene expression for phenotypic diagnosis, where further investigations should be carried out to confirm robust and dependable genetic markers of resilience and susceptibility. Advisors/Committee Members: Griffin, Frank (advisor).

Subjects/Keywords: Johne's disease; paratuberculosis

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APA (6^th Edition):

McCulloch, T. R. (n.d.). Genetic Markers to Identify the Resilient and Susceptible Phenotypes to Johne’s Disease in Red Deer (Cervus elaphus) . (Masters Thesis). University of Otago. Retrieved from http://hdl.handle.net/10523/4982

Note: this citation may be lacking information needed for this citation format:
No year of publication.

Chicago Manual of Style (16^th Edition):

McCulloch, Timothy Ryan. “Genetic Markers to Identify the Resilient and Susceptible Phenotypes to Johne’s Disease in Red Deer (Cervus elaphus) .” Masters Thesis, University of Otago. Accessed January 21, 2021. http://hdl.handle.net/10523/4982.

Note: this citation may be lacking information needed for this citation format:
No year of publication.

MLA Handbook (7^th Edition):

McCulloch, Timothy Ryan. “Genetic Markers to Identify the Resilient and Susceptible Phenotypes to Johne’s Disease in Red Deer (Cervus elaphus) .” Web. 21 Jan 2021.

Note: this citation may be lacking information needed for this citation format:
No year of publication.

Vancouver:

McCulloch TR. Genetic Markers to Identify the Resilient and Susceptible Phenotypes to Johne’s Disease in Red Deer (Cervus elaphus) . [Internet] [Masters thesis]. University of Otago; [cited 2021 Jan 21]. Available from: http://hdl.handle.net/10523/4982.

Note: this citation may be lacking information needed for this citation format:
No year of publication.

Council of Science Editors:

McCulloch TR. Genetic Markers to Identify the Resilient and Susceptible Phenotypes to Johne’s Disease in Red Deer (Cervus elaphus) . [Masters Thesis]. University of Otago; Available from: http://hdl.handle.net/10523/4982

Note: this citation may be lacking information needed for this citation format:
No year of publication.

Universidad Nacional de La Plata

13. Romero, Magalí Andrea. Implementación de un medio de cultivo líquido para el diagnóstico de paratuberculosis bovina.

Degree: 2019, Universidad Nacional de La Plata

URL: http://sedici.unlp.edu.ar/handle/10915/87228 ; https://doi.org/10.35537/10915/87228

►

La paratuberculosis bovina es una enfermedad inflamatoria intestinal crónica producida por Mycobacterium avium subsp. paratuberculosis, un bacilo acido – alcohol resistente que está relacionado con… (more)

▼

La paratuberculosis bovina es una enfermedad inflamatoria intestinal crónica producida por Mycobacterium avium subsp. paratuberculosis, un bacilo acido – alcohol resistente que está relacionado con ciertas enfermedades en el humano. El diagnóstico es la llave para el manejo sanitario y la técnica diagnóstica de oro de esta enfermedad es el cultivo bacteriológico. El medio de cultivo más utilizado es el de Herrold con micobactina (HEYM), un medio sólido con el que se tarda entre 2 y 4 meses para dar un resultado concreto y tiene una baja sensibilidad, cercana al 50%. A pesar de que con los medios líquidos se obtienen mejores resultados, para el diagnóstico de paratuberculosis los que existen son kits comerciales importados y de alto costo. Se implementó un medio de cultivo líquido no comercial, con Middlebrook 7H9 y yema de huevo. Se lo comparó con el HEYM y se demostró mediante la prueba de modelos latentes bayesianos una mayor sensibilidad del medio líquido (94%) sin ir en detrimento de la especificidad (92%), con un área bajo la curva de 0,93 en contraste con el área bajo la curva del HEYM, de 0,84. También se demostró una disminución significativa en el tiempo de incubación requerido para obtener un resultado positivo (p=7,14-12) con este medio, promediando en 19 días para el líquido y 64 para el HEYM (animales clínicos). Además, se reemplazaron los ingredientes importados, incluyendo la micobactina, logrando reducir el costo de producción de un litro de medio en más de 7000 (129,69USD/107 Kg carne pv ). La micobactina se extrajo a partir de Mycobacterium avium subsp avium cepa D4ER, utilizando el método de Snow y se demostró que la actividad biológica del extracto recuperado (micobactina nacional, MN) es equiparable a la micobactina J®, con una eficiencia similar y un costo de producción de 33 (0,57USD/0,5 Kg carne pv) /mg de MN producido.

Facultad de Ciencias Veterinarias

Advisors/Committee Members: Travería, Gabriel Eduardo, Santangelo, María de la Paz.

Subjects/Keywords: Ciencias Veterinarias; paratuberculosis; diagnóstico; cultivo líquido; micobactina

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APA (6^th Edition):

Romero, M. A. (2019). Implementación de un medio de cultivo líquido para el diagnóstico de paratuberculosis bovina. (Thesis). Universidad Nacional de La Plata. Retrieved from http://sedici.unlp.edu.ar/handle/10915/87228 ; https://doi.org/10.35537/10915/87228

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Romero, Magalí Andrea. “Implementación de un medio de cultivo líquido para el diagnóstico de paratuberculosis bovina.” 2019. Thesis, Universidad Nacional de La Plata. Accessed January 21, 2021. http://sedici.unlp.edu.ar/handle/10915/87228 ; https://doi.org/10.35537/10915/87228.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Romero, Magalí Andrea. “Implementación de un medio de cultivo líquido para el diagnóstico de paratuberculosis bovina.” 2019. Web. 21 Jan 2021.

Vancouver:

Romero MA. Implementación de un medio de cultivo líquido para el diagnóstico de paratuberculosis bovina. [Internet] [Thesis]. Universidad Nacional de La Plata; 2019. [cited 2021 Jan 21]. Available from: http://sedici.unlp.edu.ar/handle/10915/87228 ; https://doi.org/10.35537/10915/87228.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Romero MA. Implementación de un medio de cultivo líquido para el diagnóstico de paratuberculosis bovina. [Thesis]. Universidad Nacional de La Plata; 2019. Available from: http://sedici.unlp.edu.ar/handle/10915/87228 ; https://doi.org/10.35537/10915/87228

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Edinburgh

14. Mathie, Heather. Early macrophage response to Mycobacterium avium subspecies paratuberculosis.

Degree: PhD, 2018, University of Edinburgh

URL: http://hdl.handle.net/1842/31378

► Mycobacterium avium subsp. paratuberculosis (MAP) is the causative agent of Johne's disease, a chronic enteritis that has a damaging economic and welfare impact on the… (more)

▼ Mycobacterium avium subsp. paratuberculosis (MAP) is the causative agent of Johne's disease, a chronic enteritis that has a damaging economic and welfare impact on the livestock industry. Johne's disease in cattle is known to reduce milk yield and carcass value, making it of economic concern to both dairy and beef farmers. In addition, there is cause for concern regarding zoonotic transmission, as there is an unconfirmed but potential relationship between MAP infection and human Crohn's disease, which presents similar clinical symptoms. MAP is most often contracted by neonates through the faecal-oral route, but can also be spread through contact with contaminated milk and colostrum, as well as in utero. Once the host receives an oral dose, the bacteria traverse the gut epithelium and are phagocytosed by gut macrophages residing in the lamina propria and Peyer's patches. MAP are able to evade the macrophage response by resisting intracellular degradation within phagosomes. Infected macrophages respond to the infection by secreting several pro-inflammatory cytokines that drive the downstream immune response and granuloma formation. This work aimed to elucidate key early responses of bovine monocyte derived macrophages (MDM) to MAP infection, and determine the reliability of using the reference strain, K10 (which is likely to have undergone lab adaptation) to model the infection in vitro, by comparing the MDM response to K10 with the response to a recent clinical isolate, C49. At a multiplicity of infection of 5 (MOI 5), there was a significant decrease in K10 intracellular survival (~90%), compared to C49 intracellular survival, over a 24 hour infection time-course. This suggests that K10 may have lost some virulence mechanism through lab adaptation. Understanding the mechanisms of how MDM respond to these two strains could be informative for the design of targeted vaccines When further investigating the MDM response to both strains, it was found that, at MOI 5, MDM infected with K10 secreted higher levels of IL-1β and IL-10, compared to MDM infected with C49. Both cytokines are associated with mycobacterial infection and could perhaps indicate that MDM are more responsive to the K10 strain at early time-points. In addition, MDM infected with K10 produced significantly higher levels of reactive nitrogen species (RNS). RNS are antimicrobial products that can destroy invading pathogens, and have been shown to have bactericidal effects on MAP. The production of RNS could, therefore be a potential mechanism by which MDM are able to kill K10 more efficiently than C49. An additional aim of this project was to understand the importance of the route of phagocytosis in determining the outcome of MAP infection. MDM express several phagocytic receptors, including Fc receptors (FcRs), complement receptors (CR), Ctype lectin receptors and scavenger receptors. This project mainly focused on the role of the mannose receptor (MR) on bacterial uptake and downstream immune responses, as past studies have suggested that other species of…

Subjects/Keywords: 636.2; macrophages; Mycobacterium avium subsp. paratuberculosis; mycobacteria

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APA (6^th Edition):

Mathie, H. (2018). Early macrophage response to Mycobacterium avium subspecies paratuberculosis. (Doctoral Dissertation). University of Edinburgh. Retrieved from http://hdl.handle.net/1842/31378

Chicago Manual of Style (16^th Edition):

Mathie, Heather. “Early macrophage response to Mycobacterium avium subspecies paratuberculosis.” 2018. Doctoral Dissertation, University of Edinburgh. Accessed January 21, 2021. http://hdl.handle.net/1842/31378.

MLA Handbook (7^th Edition):

Mathie, Heather. “Early macrophage response to Mycobacterium avium subspecies paratuberculosis.” 2018. Web. 21 Jan 2021.

Vancouver:

Mathie H. Early macrophage response to Mycobacterium avium subspecies paratuberculosis. [Internet] [Doctoral dissertation]. University of Edinburgh; 2018. [cited 2021 Jan 21]. Available from: http://hdl.handle.net/1842/31378.

Council of Science Editors:

Mathie H. Early macrophage response to Mycobacterium avium subspecies paratuberculosis. [Doctoral Dissertation]. University of Edinburgh; 2018. Available from: http://hdl.handle.net/1842/31378

Virginia Tech

15. Tyler, Ronald Dale Jr. Evaluation of a Paratuberculosis Quantitative Polymerase Chain Reaction Assay with Microscopic Correlation.

Degree: MS, Veterinary Medical Sciences, 2012, Virginia Tech

URL: http://hdl.handle.net/10919/41736

► Paratuberculosis is an intestinal condition in ruminants infected with Mycobacterium avium subspecies paratuberculosis (MAP) and precedes Johneâ s disease, a chronic enteric disorder in ruminants… (more)

Subjects/Keywords: Johneâ s disease; paratuberculosis; histopathology; qPCR

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APA (6^th Edition):

Tyler, R. D. J. (2012). Evaluation of a Paratuberculosis Quantitative Polymerase Chain Reaction Assay with Microscopic Correlation. (Masters Thesis). Virginia Tech. Retrieved from http://hdl.handle.net/10919/41736

Chicago Manual of Style (16^th Edition):

Tyler, Ronald Dale Jr. “Evaluation of a Paratuberculosis Quantitative Polymerase Chain Reaction Assay with Microscopic Correlation.” 2012. Masters Thesis, Virginia Tech. Accessed January 21, 2021. http://hdl.handle.net/10919/41736.

MLA Handbook (7^th Edition):

Tyler, Ronald Dale Jr. “Evaluation of a Paratuberculosis Quantitative Polymerase Chain Reaction Assay with Microscopic Correlation.” 2012. Web. 21 Jan 2021.

Vancouver:

Tyler RDJ. Evaluation of a Paratuberculosis Quantitative Polymerase Chain Reaction Assay with Microscopic Correlation. [Internet] [Masters thesis]. Virginia Tech; 2012. [cited 2021 Jan 21]. Available from: http://hdl.handle.net/10919/41736.

Council of Science Editors:

Tyler RDJ. Evaluation of a Paratuberculosis Quantitative Polymerase Chain Reaction Assay with Microscopic Correlation. [Masters Thesis]. Virginia Tech; 2012. Available from: http://hdl.handle.net/10919/41736

University of Sydney

16. Bush, Russell David. The Biological And Financial Impact Of Ovine Johne’s Disease In Australia .

Degree: 2005, University of Sydney

URL: http://hdl.handle.net/2123/893

► This study was conducted to provide accurate information about the impact of OJD on sheep mortality and financial status on infected farms in Australia. Industry… (more)

▼ This study was conducted to provide accurate information about the impact of OJD on sheep mortality and financial status on infected farms in Australia. Industry considered this research to be important because little credible information was available regarding the magnitude of the OJD problem and the responses required to control and manage OJD in southern Australia. This 3-year study, conducted on 12 OJD-infected farms in southern NSW, commenced with a 12-month observational study in 2002. During this year OJD mortality estimates were derived from farm records (livestock inventories) and quarterly farm visits (necropsy inspections). Questionnaires, climatic records and pasture samples enabled a detailed description of each farm to be made and a single collection of blood and faecal samples provided OJD prevalence information for specific age cohorts of sheep in each flock. The financial impact of OJD was established using two approaches, a gross margin analysis and the provision of a financial value on the mortalities inspected during the necropsy inspection periods. For a further 2 years, inventory and management information was collected from each of the twelve farms to provide 2003 and 2004 estimates for OJD mortality and financial loss due to OJD based on gross margin analyses. A more detailed gross margin model was developed that has the capacity to compare three disease status scenarios (uninfected, infected and vaccinated) for a number of different sheep production enterprises. These enterprises include fine, medium and strong wool Merino ewes and wethers as well as 1st and 2nd cross lamb production. From the four 5-day necropsy inspections conducted in 2002, a most likely cause of death was determined for 362 necropsied sheep on the basis of findings related to the environment, clinical signs, gross pathology and histopathology. Of these, OJD was most likely to have contributed to the death of 250 sheep, OJD was unlikely to have contributed to the death of 1 sheep and OJD did not contribute to death of 111 sheep. During 2002, across the 12 farms, there were a total of 52,718 wethers and 47,374 ewes at-risk of becoming infected with OJD. The distribution of mortalities in each sex group translates to an OJD mortality rate of 4.3% among wethers and 4.9% among ewes. Distribution across inspection periods showed a trend among OJD-related necropsies and total necropsies with the majority occurring in winter (31%) and spring (35%) and fewer in autumn (18%) and summer (16%). Across the 12 farms, the annual OJD mortality rate ranged from 1.8% to 17.5% during the 3-year study with mean annual figures of 6.2% in 2002, 7.8% in 2003 and 6.4% in 2004. Of concern is the fact that these mean OJD mortality figures were all above the accepted annual mortality rate from all causes for adult sheep of 4-6% (McGregor et al., 2003) for Australian flocks. Gross margins were calculated for each of the 12 farms assuming each farm was free of OJD and then these were compared with the actual farm gross margin. The mean…

Subjects/Keywords: Paratuberculosis; Sheep; Australia

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APA (6^th Edition):

Bush, R. D. (2005). The Biological And Financial Impact Of Ovine Johne’s Disease In Australia . (Thesis). University of Sydney. Retrieved from http://hdl.handle.net/2123/893

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Bush, Russell David. “The Biological And Financial Impact Of Ovine Johne’s Disease In Australia .” 2005. Thesis, University of Sydney. Accessed January 21, 2021. http://hdl.handle.net/2123/893.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Bush, Russell David. “The Biological And Financial Impact Of Ovine Johne’s Disease In Australia .” 2005. Web. 21 Jan 2021.

Vancouver:

Bush RD. The Biological And Financial Impact Of Ovine Johne’s Disease In Australia . [Internet] [Thesis]. University of Sydney; 2005. [cited 2021 Jan 21]. Available from: http://hdl.handle.net/2123/893.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Bush RD. The Biological And Financial Impact Of Ovine Johne’s Disease In Australia . [Thesis]. University of Sydney; 2005. Available from: http://hdl.handle.net/2123/893

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Sydney

17. Johansen, Matt Daniel. The role of host lipids and exosomes in the pathogenesis of Johne’s disease .

Degree: 2017, University of Sydney

URL: http://hdl.handle.net/2123/17654

► Paratuberculosis or Johne’s disease (JD) is a chronic granulomatous enteritis of ruminants such as cattle, sheep and goats. The causative agent, Mycobacterium avium subspecies paratuberculosis… (more)

Subjects/Keywords: Mycobacterium avium subspecies paratuberculosis; pathogenesis; lipid; exosome

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APA (6^th Edition):

Johansen, M. D. (2017). The role of host lipids and exosomes in the pathogenesis of Johne’s disease . (Thesis). University of Sydney. Retrieved from http://hdl.handle.net/2123/17654

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Johansen, Matt Daniel. “The role of host lipids and exosomes in the pathogenesis of Johne’s disease .” 2017. Thesis, University of Sydney. Accessed January 21, 2021. http://hdl.handle.net/2123/17654.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Johansen, Matt Daniel. “The role of host lipids and exosomes in the pathogenesis of Johne’s disease .” 2017. Web. 21 Jan 2021.

Vancouver:

Johansen MD. The role of host lipids and exosomes in the pathogenesis of Johne’s disease . [Internet] [Thesis]. University of Sydney; 2017. [cited 2021 Jan 21]. Available from: http://hdl.handle.net/2123/17654.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Johansen MD. The role of host lipids and exosomes in the pathogenesis of Johne’s disease . [Thesis]. University of Sydney; 2017. Available from: http://hdl.handle.net/2123/17654

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of New South Wales

18. Timms, Verlaine. The mycobacteriology of inflammatory bowel disease.

Degree: Biotechnology & Biomolecular Sciences, 2014, University of New South Wales

URL: http://handle.unsw.edu.au/1959.4/53780 ; https://unsworks.unsw.edu.au/fapi/datastream/unsworks:12479/SOURCE02?view=true

► Inflammatory bowel diseases (IBD) are chronic idiopathic diseases of the gastrointestinal tract and often strike in the prime of life, remaining a lifelong burden. The… (more)

▼ Inflammatory bowel diseases (IBD) are chronic idiopathic diseases of the gastrointestinal tract and often strike in the prime of life, remaining a lifelong burden. The increasing prevalence of the two main types of IBD, Crohn’s disease (CD) and ulcerative colitis (UC) is indicative of an environmental cause. This thesis explores the mycobacteriology of IBD with emphasis on the gut pathogen, M. avium subspecies paratuberculosis. M. paratuberculosis normally infects livestock and is difficult to detect given its genetic homology with other subspecies of the M. avium complex. In addition, its fastidious growth requirements make isolation from cattle challenging and from sheep and humans extremely difficult, adding to significant controversy as to whether it infects humans. This study demonstrated a significant association between Crohn’s disease and M. paratuberculosis using three PCR assays. Further, the first human M. paratuberculosis ever reported from a patient with UC was isolated. This isolate, 43525, was characterized microbiologically and the genome sequenced on the fourth subculture. M. paratuberculosis 43525 displayed mycobactin dependency on only certain types of media, resistance to; ethambutol, rifampin, clofazamine and streptomycin and growth at 4 °C. Given 43525 was a human isolate of what is normally an animal pathogen, the genome sequence provided an opportunity to explore the subtle differences between subspecies of the MAC that infect animals and humans. One PPE along with one PE_PGRS protein was found to be present only in human MAC strains and two PPE/PE genes were unique to 43525 and a caprine M. paratuberculosis strain. The organisation and structure of the 43525 mycobactin cluster differed to other M. paratuberculosis strains providing an explanation for the in vitro phenotype. Finally, a unique mce operon was found to be flanked by two mycobactin genes in all M. paratuberculosis strains investigated. The culture method and validated PCR assays described in this work provide new methods to detect M. paratuberculosis in humans. In addition, the unique mce operon and the PPE/PE proteins highlighted, provide possible targets for diagnostic assays, vaccine candidates or treatment. Advisors/Committee Members: Neilan, Brett, Biotechnology & Biomolecular Sciences, Faculty of Science, UNSW, Mitchell, Hazel, Biotechnology & Biomolecular Sciences, Faculty of Science, UNSW.

Subjects/Keywords: Mycobacerium paratuberculosis; Mycobacteria; Inflammatory bowel disease

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Timms, V. (2014). The mycobacteriology of inflammatory bowel disease. (Doctoral Dissertation). University of New South Wales. Retrieved from http://handle.unsw.edu.au/1959.4/53780 ; https://unsworks.unsw.edu.au/fapi/datastream/unsworks:12479/SOURCE02?view=true

Chicago Manual of Style (16^th Edition):

Timms, Verlaine. “The mycobacteriology of inflammatory bowel disease.” 2014. Doctoral Dissertation, University of New South Wales. Accessed January 21, 2021. http://handle.unsw.edu.au/1959.4/53780 ; https://unsworks.unsw.edu.au/fapi/datastream/unsworks:12479/SOURCE02?view=true.

MLA Handbook (7^th Edition):

Timms, Verlaine. “The mycobacteriology of inflammatory bowel disease.” 2014. Web. 21 Jan 2021.

Vancouver:

Timms V. The mycobacteriology of inflammatory bowel disease. [Internet] [Doctoral dissertation]. University of New South Wales; 2014. [cited 2021 Jan 21]. Available from: http://handle.unsw.edu.au/1959.4/53780 ; https://unsworks.unsw.edu.au/fapi/datastream/unsworks:12479/SOURCE02?view=true.

Council of Science Editors:

Timms V. The mycobacteriology of inflammatory bowel disease. [Doctoral Dissertation]. University of New South Wales; 2014. Available from: http://handle.unsw.edu.au/1959.4/53780 ; https://unsworks.unsw.edu.au/fapi/datastream/unsworks:12479/SOURCE02?view=true

The Ohio State University

19. Spangler, Elizabeth. The diagnosis and epidemiology of paratuberculosis in dairy cattle in Central Ohio.

Degree: PhD, Graduate School, 1987, The Ohio State University

URL: http://rave.ohiolink.edu/etdc/view?acc_num=osu14873257407207

Subjects/Keywords: Biology; Paratuberculosis; Cattle

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Spangler, E. (1987). The diagnosis and epidemiology of paratuberculosis in dairy cattle in Central Ohio. (Doctoral Dissertation). The Ohio State University. Retrieved from http://rave.ohiolink.edu/etdc/view?acc_num=osu14873257407207

Chicago Manual of Style (16^th Edition):

Spangler, Elizabeth. “The diagnosis and epidemiology of paratuberculosis in dairy cattle in Central Ohio.” 1987. Doctoral Dissertation, The Ohio State University. Accessed January 21, 2021. http://rave.ohiolink.edu/etdc/view?acc_num=osu14873257407207.

MLA Handbook (7^th Edition):

Spangler, Elizabeth. “The diagnosis and epidemiology of paratuberculosis in dairy cattle in Central Ohio.” 1987. Web. 21 Jan 2021.

Vancouver:

Spangler E. The diagnosis and epidemiology of paratuberculosis in dairy cattle in Central Ohio. [Internet] [Doctoral dissertation]. The Ohio State University; 1987. [cited 2021 Jan 21]. Available from: http://rave.ohiolink.edu/etdc/view?acc_num=osu14873257407207.

Council of Science Editors:

Spangler E. The diagnosis and epidemiology of paratuberculosis in dairy cattle in Central Ohio. [Doctoral Dissertation]. The Ohio State University; 1987. Available from: http://rave.ohiolink.edu/etdc/view?acc_num=osu14873257407207

Universiteit Utrecht

20. Knupfer, E. Within-farm strain dynamics of Mycobacterium avium subsp paratuberculosis: Evidence for limited vertical transmission.

Degree: 2010, Universiteit Utrecht

URL: http://dspace.library.uu.nl:8080/handle/1874/215081

► MAP-infected dairy cattle are assumed to be a high risk for transmitting infection to their daughters. Alternatively, if both dam and daughter are genetically more… (more)

▼ MAP-infected dairy cattle are assumed to be a high risk for transmitting infection to their daughters. Alternatively, if both dam and daughter are genetically more susceptible to MAP, they may be both infected but not necessarily due to vertical transmission. Using strain typing techniques including multi locus short sequence repeat (MLSSR) typing allows a potential distinction between vertical transmission and genetic susceptibility. Analyzing strain diversity in longitudinal datasets provides additional insight into within-herd infection dynamics, including the transmission of MAP from dams to daughters. To investigate the importance of vertical transmission, we identified 12 pairs of dams and daughters for which both animals are known MAP infected from the Regional Dairy Quality Management Alliance (RDQMA) study herd in NY. All adult animals on the farm were tested for MAP via fecal culture semi-annually for seven years. Tissue samples were available on a subset of cull animals. Animals were considered MAP-infected if they ever cultured positive or if any of their tissues cultured positive at slaughter. Cultures were performed at University of Pennsylvania on HEYM solid media. Positive cultures were substreaked and processed for MLSSR typing. Following genotyping, isolates from each dam-daughter pair were compared to determine whether they shared the same MAP genotype. Environmental MAP burden at birth was assessed via typing of MAP-positive environmental samples (collected four times a year) and known MAP-infected animals present on the farm during the high-risk first year of life. Of the 12 infected dam-daughter pairs, 9 had identical strains shared between the dams and daughters. In addition, 2 daughters had the dam’s strain as well as another circulating strain. Overall, there were 8 strains represented in the daughters that did not come from dams (2 daughters had multiple strains which did not originate from the dam). These results lend additional importance to the impact of genetics on susceptibility, as 5 of 12 daughters carried different strains of MAP than their dams, even when concurrently infected with the dam’s strain. Advisors/Committee Members: Schukken, Prof. Dr. Y.H., Jorritsma, Dr. R..

Subjects/Keywords: Diergeneeskunde; Mycobacterium avium paratuberculosis, paratuberculosis, MAP, Johne's, vertical transmission, MLSSR, genetic susceptibility, transmission

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Knupfer, E. (2010). Within-farm strain dynamics of Mycobacterium avium subsp paratuberculosis: Evidence for limited vertical transmission. (Doctoral Dissertation). Universiteit Utrecht. Retrieved from http://dspace.library.uu.nl:8080/handle/1874/215081

Chicago Manual of Style (16^th Edition):

Knupfer, E. “Within-farm strain dynamics of Mycobacterium avium subsp paratuberculosis: Evidence for limited vertical transmission.” 2010. Doctoral Dissertation, Universiteit Utrecht. Accessed January 21, 2021. http://dspace.library.uu.nl:8080/handle/1874/215081.

MLA Handbook (7^th Edition):

Knupfer, E. “Within-farm strain dynamics of Mycobacterium avium subsp paratuberculosis: Evidence for limited vertical transmission.” 2010. Web. 21 Jan 2021.

Vancouver:

Knupfer E. Within-farm strain dynamics of Mycobacterium avium subsp paratuberculosis: Evidence for limited vertical transmission. [Internet] [Doctoral dissertation]. Universiteit Utrecht; 2010. [cited 2021 Jan 21]. Available from: http://dspace.library.uu.nl:8080/handle/1874/215081.

Council of Science Editors:

Knupfer E. Within-farm strain dynamics of Mycobacterium avium subsp paratuberculosis: Evidence for limited vertical transmission. [Doctoral Dissertation]. Universiteit Utrecht; 2010. Available from: http://dspace.library.uu.nl:8080/handle/1874/215081

21. Elise Miyuki Yamasaki. Clinic-pathological aspects of paratuberculosis in dairy cattle in Rio Claro county, Rio de Janeiro State.

Degree: 2010, Universidade Federal Rural do Rio de Janeiro

URL: http://bdtd.ufrrj.br//tde_busca/arquivo.php?codArquivo=1407

►

Descreve-se os aspectos epidemiológicos e clínico-patológicos da paratuberculose em um rebanho bovino leiteiro no município de Rio Claro, região Sul-Fluminense, Rio de Janeiro. No período… (more)

▼

Descreve-se os aspectos epidemiológicos e clínico-patológicos da paratuberculose em um rebanho bovino leiteiro no município de Rio Claro, região Sul-Fluminense, Rio de Janeiro. No período de 2006 a 2009, oito vacas adultas da raça Girolanda apresentaram diarreia crônico-intermitente, perda de peso progressivo e apetite normal. À necropsia observou-se vasos linfáticos subserosos proeminentes, linfonodos mesentéricos aumentados de volume, úmidos ao corte, serosa do intestino com aspecto anelado e cerebróide, mucosa espessada, pregueada e com aspecto microgranular. Em especial, no duodeno havia lesões polipóides na supefície da mucosa. À microscopia, desde o duodeno até o intestino grosso, havia acentuada inflamação granulomatosa difusa, marcada dilatação dos vasos linfáticos no ápice das vilosidades, linfangiectasia e linfangite granulomatosa na submucosa, muscular e serosa, alterações também vistas nos linfonodos mesentéricos. A coloração de Ziehl-Neelsen revelou, variável quantidade de bacilos álcool-ácido resistentes no interior de macrófagos, células gigantes de Langhans e livres na mucosa e submucosa do intestino delgado e grosso e em linfonodos mesentéricos. A lâmina própria da mucosa, principalmente do jejuno e íleo de alguns animais, apresentava acentuada hipertrofia; as lesões polipóides correspondiam à marcada hipertrofia da muscular da mucosa, hiperplasia de glândulas duodenais e intestinais. Mycobacterium avium subsp. paratuberculosis foi isolado em cultivo bacteriano a partir de amostras de fezes, raspado de mucosa intestinal e leite e identificado pela técnica de PCR IS900. Através da avaliação sorológica semestral, foram analisadas 298 vacas, a partir de três anos de idade, e observou-se cerca de 40% de animais reagentes ao teste ELISA indireto no período estudado. O diagnóstico da paratuberculose foi baseado nos dados clínicopatológicos, sorologia, isolamento e identificação do agente em amostras de fezes, raspado de mucosa e leite, através do cultivo bacteriano e PCR IS900. Após implementação de medidas de controle, tais como eliminação de animais doentes, abate seletivo dos soropositivos, separação dos bezerros ao nascer com utilização de banco de colostro, observou-se diminuição da ocorrência de casos clínicos no rebanho, de seis casos por ano para cerca de um caso por ano, em três anos de estudo.

The epidemic and clinic-pathological aspects of paratuberculois in a dairy cattle herd in the Rio Claro county, southern Rio de Janeiro region, are described. In the years 2006-2009, eight adult cows presented chronic-intermittent diarrhea, chronic weight loss and normal appetite. At necropsy, the subserosal lymphatics were proeminent and dilated, mesenteric nodes were enlarged, and intestinal mucosa was corrugated, thickened and of microgranular aspect. Especially, in duodenum, was observed polipoids lesions in mucosa surface. Histopathology revealed, from the duodenum to the rectum, severe and diffuse granulomatous inflammation of the lamina propria and submucosa, broadened and distorced villi, marked…

Advisors/Committee Members: Marilene de Farias Brito.

Subjects/Keywords: mycobacterium avium subsp. paratuberculosis; bovino; patologia.; PATOLOGIA ANIMAL; bovine; mycobacterium avium subsp. paratuberculosis; pathology.

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Yamasaki, E. M. (2010). Clinic-pathological aspects of paratuberculosis in dairy cattle in Rio Claro county, Rio de Janeiro State. (Thesis). Universidade Federal Rural do Rio de Janeiro. Retrieved from http://bdtd.ufrrj.br//tde_busca/arquivo.php?codArquivo=1407

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Yamasaki, Elise Miyuki. “Clinic-pathological aspects of paratuberculosis in dairy cattle in Rio Claro county, Rio de Janeiro State.” 2010. Thesis, Universidade Federal Rural do Rio de Janeiro. Accessed January 21, 2021. http://bdtd.ufrrj.br//tde_busca/arquivo.php?codArquivo=1407.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Yamasaki, Elise Miyuki. “Clinic-pathological aspects of paratuberculosis in dairy cattle in Rio Claro county, Rio de Janeiro State.” 2010. Web. 21 Jan 2021.

Vancouver:

Yamasaki EM. Clinic-pathological aspects of paratuberculosis in dairy cattle in Rio Claro county, Rio de Janeiro State. [Internet] [Thesis]. Universidade Federal Rural do Rio de Janeiro; 2010. [cited 2021 Jan 21]. Available from: http://bdtd.ufrrj.br//tde_busca/arquivo.php?codArquivo=1407.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Yamasaki EM. Clinic-pathological aspects of paratuberculosis in dairy cattle in Rio Claro county, Rio de Janeiro State. [Thesis]. Universidade Federal Rural do Rio de Janeiro; 2010. Available from: http://bdtd.ufrrj.br//tde_busca/arquivo.php?codArquivo=1407

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

22. Jolly, Ana. Paratuberculosis bovina : función de los anticuerpos antilipoarabinomanano en la infección.

Degree: Ciencias Veterinarias, 2016, Universidad de Buenos Aires

URL: http://repositoriouba.sisbi.uba.ar/gsdl/cgi-bin/library.cgi?a=d&c=posgrauba&cl=CL1&d=HWA_1525 ; http://repositoriouba.sisbi.uba.ar/gsdl/collect/posgrauba/index/assoc/HWA_1525.dir/1525.PDF

►

Paratuberculosis is a chronic progressive granulomatous disease affecting ruminants, and is caused by Mycobacterium avium subsp. paratuberculosis (Map). Its worldwide distribution is known, although its… (more)

▼

Paratuberculosis is a chronic progressive granulomatous disease affecting ruminants, and is caused by Mycobacterium avium subsp. paratuberculosis (Map). Its worldwide distribution is known, although its prevalence remains underestimated\ndue to the difficulties derived from it diagnostic. It also has economic and public\nhealth importance. In addition, the lack of preventive methods hampers its control\nand determines the existence of vacancies in research topics related with\nparatuberculosis.\nEven when Map is an intracellular bacterium, the presence of specific antibodies in the intestinal lumen at the moment of infection could modulate the host response. The aim of this thesis was to assess the effect of anti-lipoarabinomannan antibodies\n(LAM, main immunodominant exposed antigen on Map surface) on the response to\ninfection with opsonized Map of bovine macrophages (in vitro) and of calf ileal loops\n(in vivo). Antibodies were obtained from sera of healthy (n=2), paratuberculosis\nnaturally infected (n=3) or LAM-immunized (n=3) bovines. The level of apoptosis and TNF?/IL-10 secretion were measured in vitro. We found\na significant increase of the apoptosis level in macrophages when the infection took\nplace in the presence of specific antibodies (24.2 ± 2.9 % and 22.4 ± 0.9 % with\nantibodies from LAM-immunized or paratuberculosis infected bovines, respectively,\nvs. 17,2 ± 2,6 % with antibodies from healthy bovines). This effect was accompanied\nby an increase in the secretion of TNF? in both cases. For the in vivo model, ileal loops of three calves were inoculated with Map in PBS, with or without antibodies. The microscopic aspect of tissue sections, the bacterial presence and the local relative expression of TNF? and IL-10 were evaluated. As the\nmain result, a significant reduction in bacterial counts (CFU, colony forming units)\nwere detected in the presence of specific antibodies (2.6 ± 1.3 x 104 CFU/g and 0.4 ±\n0.3 x 104 CFU/g for anti-LAM and infected group vs. 5,4 ± 1,1 x 104 CFU/g for\nantibodies from healthy bovines). Overall, these findings show that specific antibodies modulate the effects of Map infection in vitro as well as in vivo in the bovine models studied here. They also demonstrate for the first time that apoptosis of macrophages can be modulated by antibodies. On reflection, it is possible to hypothesize that the presence of specific\nantibodies at the portal entry dampen Map survival after mucosal invasion, at least\npartially by promoting a pro-inflammatory environment, with TNF? predominance,\nand by increasing the apoptosis level of infected macrophages. These effects could positively impact in the mounted immune response and in the control of the\ninfection. Further research is needed in order to establish the relevance of antibody\nmediated immunity on paratuberculosis prevention in cattle.

Fil: Jolly, Ana. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. Buenos Aires, Argentina

La paratuberculosis es una enfermedad granulomatosa, progresiva y crónica de\nlos…

Advisors/Committee Members: Hajos, Silvia Elvira, Mundo, Silvia Leonor.

Subjects/Keywords: Paratuberculosis; Micobacterias; ¨Bovino; Anticuerpos; Funcionalidad; Bovinos; Paratuberculosis; Anticuerpos anti-arabinomanano; Infección bacteriana; Inmunología

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Jolly, A. (2016). Paratuberculosis bovina : función de los anticuerpos antilipoarabinomanano en la infección. (Thesis). Universidad de Buenos Aires. Retrieved from http://repositoriouba.sisbi.uba.ar/gsdl/cgi-bin/library.cgi?a=d&c=posgrauba&cl=CL1&d=HWA_1525 ; http://repositoriouba.sisbi.uba.ar/gsdl/collect/posgrauba/index/assoc/HWA_1525.dir/1525.PDF

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Jolly, Ana. “Paratuberculosis bovina : función de los anticuerpos antilipoarabinomanano en la infección.” 2016. Thesis, Universidad de Buenos Aires. Accessed January 21, 2021. http://repositoriouba.sisbi.uba.ar/gsdl/cgi-bin/library.cgi?a=d&c=posgrauba&cl=CL1&d=HWA_1525 ; http://repositoriouba.sisbi.uba.ar/gsdl/collect/posgrauba/index/assoc/HWA_1525.dir/1525.PDF.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Jolly, Ana. “Paratuberculosis bovina : función de los anticuerpos antilipoarabinomanano en la infección.” 2016. Web. 21 Jan 2021.

Vancouver:

Jolly A. Paratuberculosis bovina : función de los anticuerpos antilipoarabinomanano en la infección. [Internet] [Thesis]. Universidad de Buenos Aires; 2016. [cited 2021 Jan 21]. Available from: http://repositoriouba.sisbi.uba.ar/gsdl/cgi-bin/library.cgi?a=d&c=posgrauba&cl=CL1&d=HWA_1525 ; http://repositoriouba.sisbi.uba.ar/gsdl/collect/posgrauba/index/assoc/HWA_1525.dir/1525.PDF.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Jolly A. Paratuberculosis bovina : función de los anticuerpos antilipoarabinomanano en la infección. [Thesis]. Universidad de Buenos Aires; 2016. Available from: http://repositoriouba.sisbi.uba.ar/gsdl/cgi-bin/library.cgi?a=d&c=posgrauba&cl=CL1&d=HWA_1525 ; http://repositoriouba.sisbi.uba.ar/gsdl/collect/posgrauba/index/assoc/HWA_1525.dir/1525.PDF

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

23. Fernández, Bárbara. Respuesta inmune humoral inducida por proteínas de Mycobacterium avium subsp. paratuberculosis en bovinos.

Degree: Ciencias Veterinarias, 2016, Universidad de Buenos Aires

URL: http://repositoriouba.sisbi.uba.ar/gsdl/cgi-bin/library.cgi?a=d&c=posgrauba&cl=CL1&d=HWA_1251 ; http://repositoriouba.sisbi.uba.ar/gsdl/collect/posgrauba/index/assoc/HWA_1251.dir/1251.PDF

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Paratuberculosis is a chronic granulomatous enteritis of ruminants caused by Mycobacterium avium subsp. paratuberculosis (Map). The diagnosis of paratuberculosis is difficult and the current diagnostic… (more)

▼

Paratuberculosis is a chronic granulomatous enteritis of ruminants caused by Mycobacterium avium subsp. paratuberculosis (Map). The diagnosis of paratuberculosis is difficult and the current diagnostic tests are suitable for detection of the clinical phase of the infection.\nThe general aim was to characterize the total and specific (to Map and its proteins) bovine antibodies, and to identify its role in the viability of bacteria. The especial aims were: To detect the effect of the Map infection on the levels of total antibodies. To characterize the specific humoral immune response to Map and its proteins in naturally- and experimentally-infected bovines. To evaluate the role of specific bovine antibodies to Map and its proteins in the viability of ingested bacteria.\nBovine antibodies were evaluated by ELISA (levels) and by an in vitro macrophages infection model (role). A total of 120 animals were studied. The analyzed groups varied with the experiments: subclinically, clinically and terminally naturally infected, experimentally infected, exposed, immunized with specific proteins of Map and healthy control bovines.\nData demonstrated that:\nMap infection affects the total levels of antibodies in sera and feces samples of animals in the different stages of paratuberculosis: bovines with clinical signs present increased total levels of IgM in sera as well as IgM, IgG and IgA in feces. The study of whole Map or protoplasmic antigen (PPA) specific IgG2 improves the identification of subclinically infected bovines (sensitivity of 54% and 23% to IgG2 and IgG/ELISA-PPA, respectively).\nIgG2 is the first isotype that increases in sera of Map-experimentally infected calves (p=0,013, on 60 days postinfection). Map viability is decreased upon treatment with antibodies from p34r-immunized cattle in the in vitro macrophages infection model. The results impact on the current diagnostic techniques and demonstrate the functional anti-bacterial role of anti-p34r antibodies. These data justify the evaluation of anti-p34r antibodies in infection and protection models

Fil: Fernández, Bárbara. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias; Argentina

La paratuberculosis, causada por el Mycobacterium avium subsp. paratuberculosis (Map), es una patología crónica intestinal que afecta a rumiantes. El diagnóstico es engorroso y permite identificar principalmente a los bovinos en estadios avanzados (clínico).\nEl objetivo general del trabajo fue caracterizar los anticuerpos bovinos totales y específicos frente a Map y sus proteínas, e identificar su efecto en la viabilidad bacteriana. Los objetivos particulares fueron: Identificar el efecto de la infección con Map en los niveles de anticuerpos totales. Caracterizar la respuesta inmune humoral específica frente a Map y sus proteínas en sobre la viabilidad bacteriana intracelular. Los anticuerpos bovinos se estudiaron por la metodología de ELISA (niveles) y mediante un modelo de infección de macrófagos in vitro (funcionalidad). Se evaluaron un total de 120 animales. Los…

Advisors/Committee Members: Mundo, Silvia Leonor.

Subjects/Keywords: Anticuerpos; Bovinos; Paratuberculosis; Bovinos; Respuesta inmune; Proteínas; Mycobacterium avium subsp. paratuberculosis; Inmunología

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Fernández, B. (2016). Respuesta inmune humoral inducida por proteínas de Mycobacterium avium subsp. paratuberculosis en bovinos. (Thesis). Universidad de Buenos Aires. Retrieved from http://repositoriouba.sisbi.uba.ar/gsdl/cgi-bin/library.cgi?a=d&c=posgrauba&cl=CL1&d=HWA_1251 ; http://repositoriouba.sisbi.uba.ar/gsdl/collect/posgrauba/index/assoc/HWA_1251.dir/1251.PDF

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Fernández, Bárbara. “Respuesta inmune humoral inducida por proteínas de Mycobacterium avium subsp. paratuberculosis en bovinos.” 2016. Thesis, Universidad de Buenos Aires. Accessed January 21, 2021. http://repositoriouba.sisbi.uba.ar/gsdl/cgi-bin/library.cgi?a=d&c=posgrauba&cl=CL1&d=HWA_1251 ; http://repositoriouba.sisbi.uba.ar/gsdl/collect/posgrauba/index/assoc/HWA_1251.dir/1251.PDF.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Fernández, Bárbara. “Respuesta inmune humoral inducida por proteínas de Mycobacterium avium subsp. paratuberculosis en bovinos.” 2016. Web. 21 Jan 2021.

Vancouver:

Fernández B. Respuesta inmune humoral inducida por proteínas de Mycobacterium avium subsp. paratuberculosis en bovinos. [Internet] [Thesis]. Universidad de Buenos Aires; 2016. [cited 2021 Jan 21]. Available from: http://repositoriouba.sisbi.uba.ar/gsdl/cgi-bin/library.cgi?a=d&c=posgrauba&cl=CL1&d=HWA_1251 ; http://repositoriouba.sisbi.uba.ar/gsdl/collect/posgrauba/index/assoc/HWA_1251.dir/1251.PDF.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Fernández B. Respuesta inmune humoral inducida por proteínas de Mycobacterium avium subsp. paratuberculosis en bovinos. [Thesis]. Universidad de Buenos Aires; 2016. Available from: http://repositoriouba.sisbi.uba.ar/gsdl/cgi-bin/library.cgi?a=d&c=posgrauba&cl=CL1&d=HWA_1251 ; http://repositoriouba.sisbi.uba.ar/gsdl/collect/posgrauba/index/assoc/HWA_1251.dir/1251.PDF

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Universidad Nacional de La Plata

24. Moyano, Roberto Damián. Estudio de la infección natural por Mycobacterium avium subsp. paratuberculosis en bovinos nacidos en rodeos con paratuberculosis.

Degree: 2017, Universidad Nacional de La Plata

URL: http://sedici.unlp.edu.ar/handle/10915/59644 ; https://doi.org/10.35537/10915/59644

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En la presente tesis se estudió la evolución de la paratuberculosis bovina en terneros nacidos en establecimientos con presencia de la enfermedad. El objetivo de… (more)

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En la presente tesis se estudió la evolución de la paratuberculosis bovina en terneros nacidos en establecimientos con presencia de la enfermedad. El objetivo de este trabajo fue la búsqueda de marcadores de protección y/o enfermedad que permitan el diagnóstico temprano de la paratuberculosis bovina. Con este propósito se estudiaron animales nacidos de dos rodeos lecheros con una prevalencia real de 30% y 10%, y un rodeo negativo. En base a los resultados obtenidos pudimos detectar la presencia de anticuerpos específicos de forma temprana en los rodeos infectados. En el rodeo de alta prevalencia se observan anticuerpos a los 8-10 meses de edad y en el rodeo de baja prevalencia a los 16 meses, mientras que la producción de interferón gamma en sangre periférica se detecta a los 18 meses solamente en el rodeo de alta prevalencia. Junto con la presencia de anticuerpos IgG totales en los dos rodeos en animales jóvenes, se destacó la presencia del isotipo IgG2 en animales con diagnóstico positivo a la enfermedad a los 18 meses de edad. Los animales que presentaron títulos altos en serología presentaron mayores niveles de linfocitos T WC1+ activados (CD25+). Por último, se pudo observar que las citoquinas que presentaron mayores niveles de expresión pertenecieron tanto a una respuesta inmune del tipo Th1, Th17 y Th2 lo cual sugeriría que la paratuberculosis bovina desarrolla una respuesta inmunológica que involucra a las 3 respuestas. Se realizó también la genotipificación de aislamientos de M. avium subsp. paratuberculosis de los establecimientos en estudio y otros provenientes de la provincia de Buenos Aires. Los resultados obtenidos destacaron la presencia de 5 genotipos diferentes los cuales ya habían sido reportados en Argentina.

Tesis dirigida por los Dres. María Isabel Romano y Gabriel Eduardo Trevería.

Facultad de Ciencias Veterinarias

Advisors/Committee Members: Romano, María Isabel, Mundo, Silvia Raquel, Rambeaud, Magdalena, Cataldi, Ángel Adrián.

Subjects/Keywords: Ciencias Veterinarias; Bovinos; diagnostico, bovinos, paratuberculosis, respuesta inmune; Paratuberculosis; Procesos del Sistema Inmunológico

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Moyano, R. D. (2017). Estudio de la infección natural por Mycobacterium avium subsp. paratuberculosis en bovinos nacidos en rodeos con paratuberculosis. (Thesis). Universidad Nacional de La Plata. Retrieved from http://sedici.unlp.edu.ar/handle/10915/59644 ; https://doi.org/10.35537/10915/59644

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Moyano, Roberto Damián. “Estudio de la infección natural por Mycobacterium avium subsp. paratuberculosis en bovinos nacidos en rodeos con paratuberculosis.” 2017. Thesis, Universidad Nacional de La Plata. Accessed January 21, 2021. http://sedici.unlp.edu.ar/handle/10915/59644 ; https://doi.org/10.35537/10915/59644.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Moyano, Roberto Damián. “Estudio de la infección natural por Mycobacterium avium subsp. paratuberculosis en bovinos nacidos en rodeos con paratuberculosis.” 2017. Web. 21 Jan 2021.

Vancouver:

Moyano RD. Estudio de la infección natural por Mycobacterium avium subsp. paratuberculosis en bovinos nacidos en rodeos con paratuberculosis. [Internet] [Thesis]. Universidad Nacional de La Plata; 2017. [cited 2021 Jan 21]. Available from: http://sedici.unlp.edu.ar/handle/10915/59644 ; https://doi.org/10.35537/10915/59644.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Moyano RD. Estudio de la infección natural por Mycobacterium avium subsp. paratuberculosis en bovinos nacidos en rodeos con paratuberculosis. [Thesis]. Universidad Nacional de La Plata; 2017. Available from: http://sedici.unlp.edu.ar/handle/10915/59644 ; https://doi.org/10.35537/10915/59644

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Massey University

25. Smith. Systemic Mycobacterium avium subspecies paratuberculosis infection in sheep.

Degree: PhD, Veterinary Science, 2016, Massey University

URL: http://hdl.handle.net/10179/12435

► The systemic infection of organs and skeletal muscle outside the alimentary tract with Mycobacterium avium subspecies paratuberculosis (Map) has sparingly been mentioned in the many… (more)

▼ The systemic infection of organs and skeletal muscle outside the alimentary tract with Mycobacterium avium subspecies paratuberculosis (Map) has sparingly been mentioned in the many scientific studies undertaken in sheep, yet within the past decade a zoonotic association has been proposed. The occurrence of systemic Map infection at the time of slaughter might enable this organism to be present in food products, such as meat, destined for human consumption, creating a potential link to public health and may therefore attract some attention by the meat industry. There have been very few studies investigating whether meat has potential to expose humans to Map. With this lack of information, it is difficult for the meat industry to make informed decisions in the event that public perception establishes a link with Crohn’s disease. Chapter one provides a brief history of Map infection in ruminants and suggests there may be a need to identify steps that could be implemented to mitigate human exposure to Map. The aims for this thesis therefore were to i) determine whether skeletal muscle from naturally infected animals provides a source of Map for humans, ii) provide information on systemic Map infection in sheep, identifying classes of stock that may pose a risk for exposure iii) develop a histological diagnostic test for quantifying the cost of systemic Map infection in sheep with potential use in therapeutic efficacy studies, and iv) provide a potential means to mass screen sheep at time of slaughter using real time spectroscopy to identify systemically infected animals. Chapter two reviews the source of Map, transmission pathways and subsequent availability of modern diagnostic tests for identifying sheep infected with this organism. There is a lack of published information on systemic Map infection, with little known about how this event develops, how the immune system reacts when Map bacteraemia occurs, whether systemic 3 Map infection has a cost to production and whether quantification of this cost can be assessed with currently available diagnostic tests. The aim of Chapter three was to determine whether skeletal muscle from ewes with clinical Johne’s disease contained Map and therefore provided a potential source of Map for humans. Fifty one mixed-age, low body condition score ewes (1.5/5), from a farm where clinical Johne’s disease had been diagnosed, were necropsied. This included 48 ewes with Map infection confirmed by ileal BACTEC radiometric culture and 21 with clinical Johne’s disease confirmed by ileal histopathology. In 18 ewes with clinical Johne’s disease, Map was found in the culture of blood (n=13), blood and muscle (n=10) and muscle (n=5). In ewes without clinical Johne’s disease, Map was found in 5/30 animals including muscle (n=4) and blood (n=1). It was concluded that meat from ewes with clinical Johne’s disease is likely to contain Map and suggested that systemic Map infection may also occur in sheep without clinical disease when managed in direct contact with…

Subjects/Keywords: Mycobacterium avium paratuberculosis; Paratuberculosis; Epidemiology; New Zealand; Research Subject Categories::VETERINARY MEDICINE

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Smith. (2016). Systemic Mycobacterium avium subspecies paratuberculosis infection in sheep. (Doctoral Dissertation). Massey University. Retrieved from http://hdl.handle.net/10179/12435

Note: this citation may be lacking information needed for this citation format:
Author name may be incomplete

Chicago Manual of Style (16^th Edition):

Smith. “Systemic Mycobacterium avium subspecies paratuberculosis infection in sheep.” 2016. Doctoral Dissertation, Massey University. Accessed January 21, 2021. http://hdl.handle.net/10179/12435.

Note: this citation may be lacking information needed for this citation format:
Author name may be incomplete

MLA Handbook (7^th Edition):

Smith. “Systemic Mycobacterium avium subspecies paratuberculosis infection in sheep.” 2016. Web. 21 Jan 2021.

Note: this citation may be lacking information needed for this citation format:
Author name may be incomplete

Vancouver:

Smith. Systemic Mycobacterium avium subspecies paratuberculosis infection in sheep. [Internet] [Doctoral dissertation]. Massey University; 2016. [cited 2021 Jan 21]. Available from: http://hdl.handle.net/10179/12435.

Note: this citation may be lacking information needed for this citation format:
Author name may be incomplete

Council of Science Editors:

Smith. Systemic Mycobacterium avium subspecies paratuberculosis infection in sheep. [Doctoral Dissertation]. Massey University; 2016. Available from: http://hdl.handle.net/10179/12435

Note: this citation may be lacking information needed for this citation format:
Author name may be incomplete

Massey University

26. Verdugo, Cristobal. Epidemiology of Mycobacterium avium subspecies paratuberculosis infection on sheep, beef cattle and deer farms in New Zealand.

Degree: PhD, 2013, Massey University

URL: http://hdl.handle.net/10179/6927

► Paratuberculosis (Ptb) is a chronic enteric infection caused by Mycobacterium avium subspecies paratuberculosis (MAP), affecting wild and domestic ruminants. In domestic ruminants MAP infection is… (more)

▼ Paratuberculosis (Ptb) is a chronic enteric infection caused by Mycobacterium avium subspecies paratuberculosis (MAP), affecting wild and domestic ruminants. In domestic ruminants MAP infection is largely sub-clinical, but can result in chronic diarrhoea leading to emaciation and death. Clinical disease is commonly observed in adult cattle and sheep but in deer the disease incidence is higher in young animals (8-12 months). In the New Zealand pastoral farming system, it is common practice to co-graze Ptb susceptible livestock species (sheep, cattle, and deer) together, either concurrently or successively, on the same pasture. Thus several susceptible species have contact at farm level, being at risk of transmitting MAP between species through contaminated pasture. Johne’s Disease Research Consortium (JDRC), a partnership between livestock industries, government and research providers was created to study Ptb in an overarching approach, involving all susceptible species, aiming to generate scientific knowledge to support Ptb control policies. The present research was implemented under the financial support of JDRC, aiming to generate epidemiological information about Ptb infection and clinical disease on mixedspecies pastoral farms, grazing sheep, beef cattle, and/or deer. A total of 350 mixedspecies farms (11,089 animals) were faecal and blood sampled and related epidemiological information was collected. Data was used to estimate: i) the national herd level true prevalence (HTP) of MAP infection on sheep, beef cattle and deer, ii) the risk of MAP infection and clinical disease incidence associated with species co-grazing,iii) the association between infected and affected herds/flocks and production outputs, and iv) relationships between molecular strain types of MAP isolates and their distribution across livestock sectors and geographical areas. Finally, data and results from previous studies allowed v) the development and calibration of a two host-species (sheep & beef cattle) mathematical model, simulating MAP transmission between species and the effect of several control measures under mixed species farming. MAP infection is widely spread in New Zealand. A Bayesian analysis to account for lack of sensitivity (Se) and specificity (Sp) of testing protocols, indicated that the highest HTP estimate for sheep flocks (75%, posterior probability interval (PPI) 68- 82%), followed by deer (46%, PPI 39-54%) and beef herds (43%, PPI 359-51%). Sheep and beef cattle flocks/herds presented a higher prevalence in the North Island (NI), whereas deer infection was mainly located in the South Island (SI). Logistic and Poisson regression models using Bayesian inference to adjust for lack of Se and Sp of diagnostic tests and of farmer’s recall of clinical Ptb indicated that the shared use of pasture was associated with Ptb prevalence and incidence. When beef cattle and sheep were co-grazed, the infection risk increased 3-4 times in each species. Similarly, co-grazing of beef cattle and deer…

Subjects/Keywords: Paratuberculosis; Mycobacterium avium paratuberculosis; Epidemiology; New Zealand; Research Subject Categories::VETERINARY MEDICINE::Veterinary epidemiology

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Verdugo, C. (2013). Epidemiology of Mycobacterium avium subspecies paratuberculosis infection on sheep, beef cattle and deer farms in New Zealand. (Doctoral Dissertation). Massey University. Retrieved from http://hdl.handle.net/10179/6927

Chicago Manual of Style (16^th Edition):

Verdugo, Cristobal. “Epidemiology of Mycobacterium avium subspecies paratuberculosis infection on sheep, beef cattle and deer farms in New Zealand.” 2013. Doctoral Dissertation, Massey University. Accessed January 21, 2021. http://hdl.handle.net/10179/6927.

MLA Handbook (7^th Edition):

Verdugo, Cristobal. “Epidemiology of Mycobacterium avium subspecies paratuberculosis infection on sheep, beef cattle and deer farms in New Zealand.” 2013. Web. 21 Jan 2021.

Vancouver:

Verdugo C. Epidemiology of Mycobacterium avium subspecies paratuberculosis infection on sheep, beef cattle and deer farms in New Zealand. [Internet] [Doctoral dissertation]. Massey University; 2013. [cited 2021 Jan 21]. Available from: http://hdl.handle.net/10179/6927.

Council of Science Editors:

Verdugo C. Epidemiology of Mycobacterium avium subspecies paratuberculosis infection on sheep, beef cattle and deer farms in New Zealand. [Doctoral Dissertation]. Massey University; 2013. Available from: http://hdl.handle.net/10179/6927

Michigan State University

27. Roussey, Jonathan Albert. A possible role for regulatory T cells in the progression of Johne's disease.

Degree: 2015, Michigan State University

URL: http://etd.lib.msu.edu/islandora/object/etd:2923

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Thesis Ph. D. Michigan State University. Comparative Medicine and Integrative Biology 2015

Johne's disease, or paratuberculosis, is a chronic wasting disease that is caused by… (more)

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Thesis Ph. D. Michigan State University. Comparative Medicine and Integrative Biology 2015

Johne's disease, or paratuberculosis, is a chronic wasting disease that is caused by infection with Mycobacterium avium subspecies paratuberculosis (MAP). Johne's disease affects wild and domestic ruminants including livestock species such as cows. Due to factors including losses in milk production and early culling of infected animals, Johne's disease represents a major financial burden, estimated at between 250 million and 1.5 billion annually for the U.S. dairy industry alone. MAP is an obligate intracellular pathogen that is transmitted through the fecal-oral route. MAP infects the host by invading the ileum of the small intestine through M cells lining the lumen, following which it colonizes tissue-resident macrophages thereby establishing persistent infection. The immune response to MAP infection is characterized by a shift from a productive Th1 immune response to an unproductive Th2 response. As a chronic condition, MAP infection may result in the development of a regulatory T cell (Treg) population within infected animals, and these Tregs may play a role in the Th1-to-Th2 immune shift observed in animals progressing from subclinical to clinical disease. Alternatively, Tregs may be critical for controlling chronic inflammation, and their loss may result in severe widespread inflammation that results in damage to host tissues and progression into clinical disease. In this project, we sought to investigate the relationship between MAP infection and Treg activity. First, we utilized a monocyte-derived macrophage (MDM) model of MAP infection in an effort to induce the development of a Treg phenotype in naïve T cells from cows with Johne's disease. Second, we developed a method to expand the relative abundance of Tregs present in peripheral blood mononuclear cell (PBMC) populations from MAP-infected cows. Following this, expanded Tregs were used in functional assays to determine if they dampened Th1 immune responses to stimulation of PBMCs with live MAP. Finally, we developed a scheme to classify lesions from cows with Johne's disease according to histopathology and abundance of MAP within ileal and mesenteric lymph tissues. Based on this system, we graded lesions from cows with Johne's disease and measured several variables, including relative Treg abundance, within these lesions as compared to healthy control tissues. Although a Treg phenotype did not develop in naïve T cells cultured with MAP-infected MDMs, we observed a state of T cell unresponsiveness in naïve T cells from cows with clinical disease, and a state of reduced responsiveness in naïve T cells from cows with subclinical disease, as compared to responses from control cows. In our second set of experiments, we found that we were able to successfully expand bovine Tregs, and although these expanded Tregs are not MAP-specific, they are functional and do suppress Th1 immune activity generally. Within infected tissues we found that cows with clinical disease…

Advisors/Committee Members: Coussens, Paul M, Harkema, Jack R, Mansfield, Linda S, Parameswaran, Narayanan, Sordillo, Lorraine M.

Subjects/Keywords: Paratuberculosis – Immunological aspects; T cells; Mycobacterium avium paratuberculosis; Immunology; Pathology; Animal diseases

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Roussey, J. A. (2015). A possible role for regulatory T cells in the progression of Johne's disease. (Thesis). Michigan State University. Retrieved from http://etd.lib.msu.edu/islandora/object/etd:2923

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Roussey, Jonathan Albert. “A possible role for regulatory T cells in the progression of Johne's disease.” 2015. Thesis, Michigan State University. Accessed January 21, 2021. http://etd.lib.msu.edu/islandora/object/etd:2923.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Roussey, Jonathan Albert. “A possible role for regulatory T cells in the progression of Johne's disease.” 2015. Web. 21 Jan 2021.

Vancouver:

Roussey JA. A possible role for regulatory T cells in the progression of Johne's disease. [Internet] [Thesis]. Michigan State University; 2015. [cited 2021 Jan 21]. Available from: http://etd.lib.msu.edu/islandora/object/etd:2923.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Roussey JA. A possible role for regulatory T cells in the progression of Johne's disease. [Thesis]. Michigan State University; 2015. Available from: http://etd.lib.msu.edu/islandora/object/etd:2923

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Swedish University of Agricultural Sciences

28. Herthnek, David. Molecular diagnostic methods for Mycobacterium avium subsp. paratuberculosis.

Degree: 2009, Swedish University of Agricultural Sciences

URL: http://pub.epsilon.slu.se/1954/

► Mycobacterium avium subsp. paratuberculosis (MAP) is the causative agent of the chronic enteric disease paratuberculosis in ruminants that causes considerable economic losses worldwide. Due to… (more)

▼ Mycobacterium avium subsp. paratuberculosis (MAP) is the causative agent of the chronic enteric disease paratuberculosis in ruminants that causes considerable economic losses worldwide. Due to rigorous control measures, paratuberculosis is rare or absent in Sweden. However, import-related outbreaks have occurred. Diagnostic surveillance and outbreak investigations are mainly carried out by very slow culture methods. Faster and equally reliable molecular methods are needed for detection of MAP in several clinical matrixes. MAP is primarily shed in faeces, the most important testing material. The abundance of PCR inhibitory substances in faeces constitutes a diagnostic challenge. Semen, imported for breeding purposes, may contain MAP if the donor bulls are asymptomatic carriers. MAP has been suggested as the causative agent of Crohn's disease and its presence in consumers' milk might be of concern. In this thesis, the development and sensitivity assessment of protocols for detection of MAP in ruminant faeces, semen and milk by real-time PCR are described. The analytical sensitivities were assessed to 104 MAP/g faeces, 10 MAP/100 µl semen and 100 MAP/ml milk. The faeces direct PCR was validated on 202 proficiency test samples. MAP was detected in 97% of previously frozen positive samples - better than culture. Pellet and cream fractions of milk were pooled before cell lysis and DNA extraction by automated magnetic bead separation. In a study of 56 dairy herds, tank milk PCR was compared to culture of environmental faecal samples for herd prevalence testing. By the latter, 68% of the herds were positive, while 30% were positive by PCR. Due to the concluded low abundance of MAP in milk tanks, milk PCR would be more useful for testing of MAP in consumers’ milk, than for herd prevalence testing. Three real-time PCR systems were designed for confirmation of PCR positives and validated on 267 strains and 58 positive faecal and tissue samples. The system based on the gene F57 was the most specific. A faecal culture screening of 501 wild guanacos in Chile yielded MAP colonies from 21 guanacos (4.2%), representing the first isolation of MAP from wild animals in the Chilean Patagonia. Confirmation was done by PCR and typing was performed by PCR-REA. All strains proved to be of C type.

Subjects/Keywords: cattle; mycobacterium avium subsp paratuberculosis; paratuberculosis; diagnosis; pcr; microbiology; cattle; confirmation; detection; diagnosis; F57; IS900; Johne’s disease; microbiology; Mycobacterium avium subsp. paratuberculosis; real-time PCR

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Herthnek, D. (2009). Molecular diagnostic methods for Mycobacterium avium subsp. paratuberculosis. (Doctoral Dissertation). Swedish University of Agricultural Sciences. Retrieved from http://pub.epsilon.slu.se/1954/

Chicago Manual of Style (16^th Edition):

Herthnek, David. “Molecular diagnostic methods for Mycobacterium avium subsp. paratuberculosis.” 2009. Doctoral Dissertation, Swedish University of Agricultural Sciences. Accessed January 21, 2021. http://pub.epsilon.slu.se/1954/.

MLA Handbook (7^th Edition):

Herthnek, David. “Molecular diagnostic methods for Mycobacterium avium subsp. paratuberculosis.” 2009. Web. 21 Jan 2021.

Vancouver:

Herthnek D. Molecular diagnostic methods for Mycobacterium avium subsp. paratuberculosis. [Internet] [Doctoral dissertation]. Swedish University of Agricultural Sciences; 2009. [cited 2021 Jan 21]. Available from: http://pub.epsilon.slu.se/1954/.

Council of Science Editors:

Herthnek D. Molecular diagnostic methods for Mycobacterium avium subsp. paratuberculosis. [Doctoral Dissertation]. Swedish University of Agricultural Sciences; 2009. Available from: http://pub.epsilon.slu.se/1954/

29. Lefrançois, Louise. Etude des adhésines HBHA et LBP impliquées dans l'interaction de Mycobacterium avium ssp. paratuberculosis avec les cellules épithéliales intestinales, cibles privilégiées de la bactérie in vivo : Characterization of HBHA and LBP adhesins involved in the interaction of Mycobacterium avium subsp. paratuberculosis with intestinal epithelial cells, the preferential target in vivo.

Degree: Docteur es, Sciences de la vie, 2012, Université François-Rabelais de Tours

URL: http://www.theses.fr/2012TOUR4020

►

Mycobacterium avium ssp. paratuberculosis (Map), agent étiologique de la paratuberculose, a évolué en deuxtypes dénommés, S pour« Sheep » et C pour « Cattle ».… (more)

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Mycobacterium avium ssp. paratuberculosis (Map), agent étiologique de la paratuberculose, a évolué en deuxtypes dénommés, S pour« Sheep » et C pour « Cattle ». L’intestin grêle est le site primaire de l’infection à Map mais les mécanismes moléculaires impliqués dans l’implantation du bacille restent largement méconnus. L’objectif de mon projet de thèse visait à identifier et caractériser les adhésines exprimées par Map par des approches génétiques et biochimiques. J’ai ainsi purifié la HBHA et la LBP par chromatographie d’affinité puis les ai identifiés en spectrométrie de masse. L’originalité de ce travail repose sur le polymorphisme de ces adhésines observé entre les souches de type C et S. Cette variabilité a été mise en évidence sur le domaine d’interaction avec les sucres sulfatés de la cellule hôte influençant l’affinité des adhésines pour l’héparine. Ce travail de thèse a permis de caractériser pour la première fois ces deux adhésines produites par Map. Le polymorphisme de la HBHA et de la LBP, discriminant les types C et S, ouvre de nombreuses perspectives sur l’évolution de l’espèce M. avium et le rôle de ces adhésines sur le tropisme intestinal, la préférence d’hôte de Map ou encore leur potentiel diagnostic.

Mycobacterium avium subsp. paratuberculosis (Map), the etiological agent of paratuberculosis, has evolved into two types called, S for "Sheep" and C for "Cattle." The small intestine is the primary site of Map infection but the molecular mechanisms involved in the establishment of bacilli are still unknown. The aim of my thesis was to identify and characterize the adhesins expressed by Map by genetic and biochemical approaches. I purified HBHA and LBP by affinity chromatography then identified them by mass spectrometry. The originality of this work is based on the polymorphism of these adhesins observed between strains of type C and S. This variability has been demonstrated in the binding domain involved in interaction with sulfated sugars of host cell influences adhesins affinity for heparin. This thesis has characterized for the first time these two adhesins produced by Map. Specific polymorphism highlighted related to the evolution of the species avium, opens large number questions on their role on the pathogenesis of Map including the cellular tropism, host preference or interest of these antigens to improve diagnostic.

Advisors/Committee Members: Biet, Franck (thesis director).

Subjects/Keywords: Paratuberculose; Mycobacterium avium ssp; Paratuberculosis; Adhésines; Heparin Binding HemAgglutinin (HBHA); Laminin Binding Protein (LBP); Polymorphisme; Paratuberculosis; Mycobacterium avium subsp; Paratuberculosis; Adhesins; Heparin Binding HemAgglutinin (HBHA); Laminin Binding Protein (LBP); Polymorphism

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Lefrançois, L. (2012). Etude des adhésines HBHA et LBP impliquées dans l'interaction de Mycobacterium avium ssp. paratuberculosis avec les cellules épithéliales intestinales, cibles privilégiées de la bactérie in vivo : Characterization of HBHA and LBP adhesins involved in the interaction of Mycobacterium avium subsp. paratuberculosis with intestinal epithelial cells, the preferential target in vivo. (Doctoral Dissertation). Université François-Rabelais de Tours. Retrieved from http://www.theses.fr/2012TOUR4020

Chicago Manual of Style (16^th Edition):

Lefrançois, Louise. “Etude des adhésines HBHA et LBP impliquées dans l'interaction de Mycobacterium avium ssp. paratuberculosis avec les cellules épithéliales intestinales, cibles privilégiées de la bactérie in vivo : Characterization of HBHA and LBP adhesins involved in the interaction of Mycobacterium avium subsp. paratuberculosis with intestinal epithelial cells, the preferential target in vivo.” 2012. Doctoral Dissertation, Université François-Rabelais de Tours. Accessed January 21, 2021. http://www.theses.fr/2012TOUR4020.

MLA Handbook (7^th Edition):

Lefrançois, Louise. “Etude des adhésines HBHA et LBP impliquées dans l'interaction de Mycobacterium avium ssp. paratuberculosis avec les cellules épithéliales intestinales, cibles privilégiées de la bactérie in vivo : Characterization of HBHA and LBP adhesins involved in the interaction of Mycobacterium avium subsp. paratuberculosis with intestinal epithelial cells, the preferential target in vivo.” 2012. Web. 21 Jan 2021.

Vancouver:

Lefrançois L. Etude des adhésines HBHA et LBP impliquées dans l'interaction de Mycobacterium avium ssp. paratuberculosis avec les cellules épithéliales intestinales, cibles privilégiées de la bactérie in vivo : Characterization of HBHA and LBP adhesins involved in the interaction of Mycobacterium avium subsp. paratuberculosis with intestinal epithelial cells, the preferential target in vivo. [Internet] [Doctoral dissertation]. Université François-Rabelais de Tours; 2012. [cited 2021 Jan 21]. Available from: http://www.theses.fr/2012TOUR4020.

Council of Science Editors:

Lefrançois L. Etude des adhésines HBHA et LBP impliquées dans l'interaction de Mycobacterium avium ssp. paratuberculosis avec les cellules épithéliales intestinales, cibles privilégiées de la bactérie in vivo : Characterization of HBHA and LBP adhesins involved in the interaction of Mycobacterium avium subsp. paratuberculosis with intestinal epithelial cells, the preferential target in vivo. [Doctoral Dissertation]. Université François-Rabelais de Tours; 2012. Available from: http://www.theses.fr/2012TOUR4020

Freie Universität Berlin

30. Gierke, Franziska. Detection of factors influencing the intra vitam diagnosis of paratuberculosis.

Degree: 2010, Freie Universität Berlin

URL: https://refubium.fu-berlin.de/handle/fub188/12881

► The diagnosis of paratuberculosis is possible via direct and indirect verification procedures. Today cultivation of faeces for the detection of the causative agent Mycobacterium avium… (more)

▼ The diagnosis of paratuberculosis is possible via direct and indirect verification procedures. Today cultivation of faeces for the detection of the causative agent Mycobacterium avium subspecies paratuberculosis (Map) and its molecular confirmation is the most recommended, highly specific intra vitam goldstandard method for the validation of serological paratuber-culosis tests. In the present thesis results of faecal cultivation and diagnostic findings of two blood and milk ELISA tests were compared and evaluated. Faeces, blood and milk samples were collected in 3 Thuringian dairy farms. Methodical effects and influences emanated from the animals that potentially affected the diagnostic techniques were analyzed. An impact of the following methodical factors on the faecal cultivation of Map was observed: storage of faecal samples, length of Hexadecylpyridiniumchlorid decontamination and number of culture slants used per faecal sample. Especially a higher number of culture slants increased the sensitivity of Map-cultivation. In one of the examined paratuberculosis herds influences on faecal culture results caused by the tested animals were not detectable, while impacts of the age and lactation stage on the cultivation of Map were determined in the other herd. Verification of Map in infected animals was significantly higher in animals older than 4 years and during the 3rd lactation stage. Additionally, shedding of Map was detected more frequently in animals with reduced milk yields (< 25 kg). The repetition of faecal culture raised the number of detected shedders and the sensitivity of the method significantly. The sensitivity of two blood and milk ELISA tests (Svanovir- and Pourquier-ELISA) proved to be insufficient in subclinically infected animals. The examined cattle population, the intensity of faecal shedding as well as the age of the animals influenced sensitivities of both serological tests. A continuing raise of seropositive test results was noticed with increasing age of infected animals. The proportion of positive serological test results was significantly higher in animals that were heavily shedding Map and for animals with daily milk yields between 5 and 35 kg. Moreover, an increase of the Svanovir milk ELISA sensitivity was detected for animals tested during the 3rd lactation stage. The diagnostic findings in a paratuberculosis nonsuspicious herd were used for the calculation of the ELISA specificity. For the Pourquier-ELISA sufficient specificities were calculated that were not significantly influenced by the animals properties. Test specificities of the Svanovir-ELISA turned out to be deficient and were additionally reduced by an increasing age of the examined cattle. Furthermore, the specificity of the Svanovir milk ELISA decreased when the daily milk yield of the tested animals was lower than 25 kg or when samples were taken during the 3rd or 4th lactation period. Methodical effects on the serological milk tests caused by the milk ingredients were possible and could not be excluded, though the… Advisors/Committee Members: w (gender), PD Dr. Dr. Petra Reinhold (firstReferee), Univ.-Prof. Dr. Kerstin E. Müller (furtherReferee), Univ.-Prof. Dr. Uwe Rösler (furtherReferee).

Subjects/Keywords: Paratuberculosis; Mycobacterium avium subsp. Paratuberculosis; diagnostic techniques; cattle diseases; elisa, faeces; serology; 600 Technik, Medizin, angewandte Wissenschaften::630 Landwirtschaft

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Gierke, F. (2010). Detection of factors influencing the intra vitam diagnosis of paratuberculosis. (Thesis). Freie Universität Berlin. Retrieved from https://refubium.fu-berlin.de/handle/fub188/12881

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Gierke, Franziska. “Detection of factors influencing the intra vitam diagnosis of paratuberculosis.” 2010. Thesis, Freie Universität Berlin. Accessed January 21, 2021. https://refubium.fu-berlin.de/handle/fub188/12881.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Gierke, Franziska. “Detection of factors influencing the intra vitam diagnosis of paratuberculosis.” 2010. Web. 21 Jan 2021.

Vancouver:

Gierke F. Detection of factors influencing the intra vitam diagnosis of paratuberculosis. [Internet] [Thesis]. Freie Universität Berlin; 2010. [cited 2021 Jan 21]. Available from: https://refubium.fu-berlin.de/handle/fub188/12881.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Gierke F. Detection of factors influencing the intra vitam diagnosis of paratuberculosis. [Thesis]. Freie Universität Berlin; 2010. Available from: https://refubium.fu-berlin.de/handle/fub188/12881

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Open Access Theses and Dissertations