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Texas A&M University
1.
Kinder, Meghan Nichole.
Campylobacter rectus Bacterial-Host Interactions in Pathogenesis.
Degree: MS, Genetics, 2017, Texas A&M University
URL: http://hdl.handle.net/1969.1/173048 
► Campylobacter rectus is a Gram-negative oral anaerobe and a causative agent of periodontitis. Additionally, C. rectus strains have been isolated from patients with oral and…
(more)
▼ Campylobacter rectus is a Gram-negative
oral anaerobe and a causative agent of periodontitis. Additionally, C. rectus strains have been isolated from patients with
oral and extraoral abscesses, gingival crevices, and appendicitis. This organism has been
implicated in bacteremia and is associated with pre-term births and low birth weight, indicating its importance as an emerging pathogen. The goal of this thesis was to establish genetic tools to begin clarifying mechanisms responsible for C. rectus
pathogenesis. In particular, secretion systems identified as important for pathogenesis in related bacterial species were selected for further study. Bacterial-host interaction assays
were used to identify host responses stimulated by the CiaB protein as well as the role of the Hcp protein in host cell adherence. Toward this goal, complete deletions of the ciaB
and the hcp genes were generated in C. rectus and reference genes for RT-qPCR in C. rectus were validated to permit assessment and verification of the mutant strains. Host
response was assessed by exposing a human placental epithelial cell line, BeWo, to both C. rectus wild-type and ciaB mutant strains for six-hours under anaerobic conditions and
inflammatory mRNA expression was measured using RT-qPCR. A significant upregulation in 41 inflammatory genes was measured in response to ΔciaB while only three were significantly upregulated in response to wild-type. THP-1 human monocyte cell viability was also assessed over a 96-hour time course after exposure to wild-type and ΔciaB. Additionally, adherence efficiencies of wild-type and Δhcp to BeWo cells were calculated after a one-hour time point. Adherence rates between wild-type and Δhcp were not significantly different. The studies in this thesis suggest C. rectus uses CiaB as a mechanism to evade the host cell immune response. Analysis of the Δhcp mutant prepared this strain for further studies like those used for the CiaB mutant strain
to fully characterize the importance of the type VI secretion system to C. rectus
virulence. Results from this foundational thesis research will expedite future studies
examining the underlying molecular mechanisms of C. rectus pathogenesis.
Advisors/Committee Members: Threadgill, Deborah S (advisor), Gill, Jason J (committee member), Sorg, Joseph A (committee member), Turner, Nancy D (committee member).
Subjects/Keywords: bacteria; pathogenesis; periodontal disease; oral pathogen
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APA (6th Edition):
Kinder, M. N. (2017). Campylobacter rectus Bacterial-Host Interactions in Pathogenesis. (Masters Thesis). Texas A&M University. Retrieved from http://hdl.handle.net/1969.1/173048
Chicago Manual of Style (16th Edition):
Kinder, Meghan Nichole. “Campylobacter rectus Bacterial-Host Interactions in Pathogenesis.” 2017. Masters Thesis, Texas A&M University. Accessed April 17, 2021.
http://hdl.handle.net/1969.1/173048.
MLA Handbook (7th Edition):
Kinder, Meghan Nichole. “Campylobacter rectus Bacterial-Host Interactions in Pathogenesis.” 2017. Web. 17 Apr 2021.
Vancouver:
Kinder MN. Campylobacter rectus Bacterial-Host Interactions in Pathogenesis. [Internet] [Masters thesis]. Texas A&M University; 2017. [cited 2021 Apr 17].
Available from: http://hdl.handle.net/1969.1/173048.
Council of Science Editors:
Kinder MN. Campylobacter rectus Bacterial-Host Interactions in Pathogenesis. [Masters Thesis]. Texas A&M University; 2017. Available from: http://hdl.handle.net/1969.1/173048
Univerzitet u Beogradu
2.
Toljić, Boško, 1984-.
Uticaj HIV infekcije i antiretrovirusnih lekova na
starenje parodontalnih tkiva.
Degree: Stomatološki fakultet, 2019, Univerzitet u Beogradu
URL: https://fedorabg.bg.ac.rs/fedora/get/o:19335/bdef:Content/get
► Stomatološke nauke - Bazične stomatološke nauke / Dental sciences - Basic dental sciences
Starenje parodontalnih tkiva, kao i čitavog organizma, je prirodan, nezaustavljiv proces koji…
(more)
▼ Stomatološke nauke - Bazične stomatološke nauke /
Dental sciences - Basic dental sciences
Starenje parodontalnih tkiva, kao i čitavog
organizma, je prirodan, nezaustavljiv proces koji se karakteriše
nakupljanjem neželjenih promena koje dovode do poremećaja
homeostaze, gubitka funkcije i većom verovatnoćom za pojavu
oštećenja, bolesti i smrti jedinke. Dinamika ovog procesa se
razlikuje među jedinkama, ali postoje razlike i u samom organizmu
jer organi, tkiva, ćelije, pa čak i subcelularne strukture ne stare
istom brzinom. Posledično, biološka starost jedinke ne mora da
odgovara njenoj hronološkoj starosti. Dalje, pojedina stanja i
patološki procesi mogu dovesti do ubrzanog starenja. Pokazano je da
osobe zaražene virusom humane imunodeficijencije (engl. Human
Immunodeficiency Virus, HIV) fenotipski brže stare od neinficiranih
osoba što može da bude posledica same infekcije i/ili primene
antiretrovirusnih lekova, koji su značajno produžili životni vek
pacijentima sa HIV-om. Upotrebom različitih markera starenja moguće
je razlikovati biološku od hronološke starosti individue, ali i
razlikovati prerano starenje odnosno bolesti koje su u vezi sa
starenjem od fiziološkog starenja. Cilj ovog istraživanja bio je da
se utvrdi veza između starenja parodontalnih tkiva i različitih
bioloških markera starenja kao što su dužina telomera, parametri
metabolizma gvožđa i dobno zavisna smena prisustva
parodontopatogena kod HIV-om inficiranih pacijenata i neinficiranih
kontrola. Istraživanje je dizajnirano kao studija preseka. U
studiju je bilo uključeno 105 HIV-om inficiranih i 100
neinficiranih ispitanika muškog pola podeljenih u dve starosne
grupe: mlađu sa ispitanicima do 35 godina života i stariju sa
ispitanicima koji imaju više od 50 godina. Nakon potpisanog
informisanog pristanka od ispitanika su dobijeni anamnestički
podaci, uzorci krvi i bris oralne sluzokože, a odrađen je i
detaljan stomatološki pregled. Iz uzoraka krvi određivani su
relativna dužina telomera leukocita i parametri metabolizma gvožđa,
serumsko gvožđe, ukupni kapacitet transferina za vezivanje gvožđa,
saturacija transferina i koncentracije transferina i feritina. Iz
briseva oralne sluzokože detektovani su sledeći parodontopatogeni
mikroorganizmi: Prevotella intermedia, Peptostreptococcus micros,
Aggregatibacter actinomycetemcomitans, Eikenella corrodens,
Porphyromonas gingivalis, Tannerella forsythia i Treponema
denticola. Inspekcijom usne duplje utvrđivano je prisustvo oralnih
manifestacija karakterističnih za HIV-infekciju dok je
parodontološki pregled poslužio za procenu stanja potpornog aparata
zuba...
Advisors/Committee Members: Milašin, Jelena, 1957-.
Subjects/Keywords: HIV; aging; periodontal tissues; oral
bacteria
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APA (6th Edition):
Toljić, Boško, 1. (2019). Uticaj HIV infekcije i antiretrovirusnih lekova na
starenje parodontalnih tkiva. (Thesis). Univerzitet u Beogradu. Retrieved from https://fedorabg.bg.ac.rs/fedora/get/o:19335/bdef:Content/get
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Toljić, Boško, 1984-. “Uticaj HIV infekcije i antiretrovirusnih lekova na
starenje parodontalnih tkiva.” 2019. Thesis, Univerzitet u Beogradu. Accessed April 17, 2021.
https://fedorabg.bg.ac.rs/fedora/get/o:19335/bdef:Content/get.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Toljić, Boško, 1984-. “Uticaj HIV infekcije i antiretrovirusnih lekova na
starenje parodontalnih tkiva.” 2019. Web. 17 Apr 2021.
Vancouver:
Toljić, Boško 1. Uticaj HIV infekcije i antiretrovirusnih lekova na
starenje parodontalnih tkiva. [Internet] [Thesis]. Univerzitet u Beogradu; 2019. [cited 2021 Apr 17].
Available from: https://fedorabg.bg.ac.rs/fedora/get/o:19335/bdef:Content/get.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Toljić, Boško 1. Uticaj HIV infekcije i antiretrovirusnih lekova na
starenje parodontalnih tkiva. [Thesis]. Univerzitet u Beogradu; 2019. Available from: https://fedorabg.bg.ac.rs/fedora/get/o:19335/bdef:Content/get
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
3.
林田, 咲.
The effect of tooth brushing, irrigation, and topical tetracycline administration on the reduction of oral bacteria in mechanically ventilated patients: a preliminary study : 人工呼吸器装着患者における歯ブラシと洗浄およびテトラサイクリンの局所塗布による口腔内細菌数の減少効果：予備的研究.
Degree: 博士(歯学), 2016, Nagasaki University / 長崎大学
URL: http://hdl.handle.net/10069/37152
► Background: One of the main causes of ventilator-associated pneumonia (VAP) is thought to be aspiration of oropharyngeal fluid containing pathogenic microorganisms. The aim of this…
(more)
▼ Background: One of the main causes of ventilator-associated pneumonia (VAP) is thought to be aspiration of oropharyngeal fluid containing pathogenic microorganisms. The aim of this study was to examine the effects of various oral care methods on the reduction of oral bacteria during intubation.
Methods: First, the effect of mechanical oral cleaning was investigated. The bacterial count on the tongue and in the oropharyngeal fluid was measured after tooth brushing, irrigation, and three hours after irrigation in mechanically ventilated patients at the intensive care unit (ICU). Next, the efficacy of topical administration of tetracycline and povidone iodine on the inhibition of bacterial growth on the tongue and in the oropharyngeal fluid was examined in oral cancer patients during neck dissection. Results: The number of bacteria in the oropharyngeal fluid was approximately 105–106 cfu/mL before surgery, but increased to 108 cfu/mL after intubation. Oral care with tooth brushing and mucosal cleaning did not reduce oral bacteria, while irrigation of the oral cavity and oropharynx significantly decreased it to a level of 105 cfu/mL (p < 0.001). However, oral bacteria increased again to almost 108 cfu/mL within three hours of irrigation. Oral bacteria did not decrease by topical povidone iodine application. In contrast, 30 min after topical administration of tetracycline, the number of oral bacteria decreased to 105 cfu/mL, and remained under 106 cfu/mL throughout the entire experimental period of 150 min.
Conclusions: While the present studies are only preliminary, these results indicate that irrigation of the oral cavity and oropharynx followed by topical antibiotic administration may reduce oral bacteria in mechanically ventilated patients.
Subjects/Keywords: Ventilator-associated pneumonia; Oral bacteria; Oral care; Irrigation; Topical antibiotic administration
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APA (6th Edition):
林田, . (2016). The effect of tooth brushing, irrigation, and topical tetracycline administration on the reduction of oral bacteria in mechanically ventilated patients: a preliminary study : 人工呼吸器装着患者における歯ブラシと洗浄およびテトラサイクリンの局所塗布による口腔内細菌数の減少効果：予備的研究. (Thesis). Nagasaki University / 長崎大学. Retrieved from http://hdl.handle.net/10069/37152
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
林田, 咲. “The effect of tooth brushing, irrigation, and topical tetracycline administration on the reduction of oral bacteria in mechanically ventilated patients: a preliminary study : 人工呼吸器装着患者における歯ブラシと洗浄およびテトラサイクリンの局所塗布による口腔内細菌数の減少効果：予備的研究.” 2016. Thesis, Nagasaki University / 長崎大学. Accessed April 17, 2021.
http://hdl.handle.net/10069/37152.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
林田, 咲. “The effect of tooth brushing, irrigation, and topical tetracycline administration on the reduction of oral bacteria in mechanically ventilated patients: a preliminary study : 人工呼吸器装着患者における歯ブラシと洗浄およびテトラサイクリンの局所塗布による口腔内細菌数の減少効果：予備的研究.” 2016. Web. 17 Apr 2021.
Vancouver:
林田 . The effect of tooth brushing, irrigation, and topical tetracycline administration on the reduction of oral bacteria in mechanically ventilated patients: a preliminary study : 人工呼吸器装着患者における歯ブラシと洗浄およびテトラサイクリンの局所塗布による口腔内細菌数の減少効果：予備的研究. [Internet] [Thesis]. Nagasaki University / 長崎大学; 2016. [cited 2021 Apr 17].
Available from: http://hdl.handle.net/10069/37152.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
林田 . The effect of tooth brushing, irrigation, and topical tetracycline administration on the reduction of oral bacteria in mechanically ventilated patients: a preliminary study : 人工呼吸器装着患者における歯ブラシと洗浄およびテトラサイクリンの局所塗布による口腔内細菌数の減少効果：予備的研究. [Thesis]. Nagasaki University / 長崎大学; 2016. Available from: http://hdl.handle.net/10069/37152
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
4.
船原, まどか.
Efficacy of topical antibiotic administration on the inhibition of perioperative oral bacterial growth in oral cancer patients: a preliminary study : 抗菌薬局所投与による口腔がん術後患者の口腔内細菌数増殖抑制効果：予備的研究.
Degree: 博士(歯学), 2016, Nagasaki University / 長崎大学
URL: http://hdl.handle.net/10069/36834
► Parenteral antibiotic prophylaxis is the current standard of therapy in cleancontaminated oral cancer surgery. Nevertheless, the incidence of surgical site infection (SSI) in oral oncological…
(more)
▼ Parenteral antibiotic prophylaxis is the current standard of therapy in cleancontaminated oral cancer surgery. Nevertheless, the incidence of surgical site
infection (SSI) in oral oncological surgery is relatively high, especially in major
surgery with reconstruction and tracheotomy. The aims of this study were to investigate the perioperative condition related to microorganisms in the oral cavity and to examine the efficacy of the topical administration of tetracycline in reducing
the number of bacteria in the oropharyngeal fluid during intubation. The number of
oral bacteria was measured during intubation in patients undergoing major oral cancer surgery. The efficacy of the topical administration of tetracycline or povidone iodine gel in reducing the bacteria was then investigated. Bacteria in the oropharyngeal fluid grew from 106 CFU/ml to 108 CFU/ml during the 3 h after intubation (CFU, colony-forming units). When tetracycline was applied to the dorsum of the tongue, oral bacteria decreased immediately to 105 CFU/ml, and the number of bacteria in the oropharyngeal fluid was maintained below 107 CFU/ml for 7 h. The concentration of tetracycline in the oropharyngeal fluid was extremely high for several hours after topical administration. The topical administration of tetracycline could reduce oral bacteria in patients undergoing clean-contaminated oral cancer surgery. This method is expected to be effective in the prevention of SSI.
Subjects/Keywords: surgical site infection; topical administration of antibiotics; oral cancer surgery; oral care; oral bacteria
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APA (6th Edition):
船原, . (2016). Efficacy of topical antibiotic administration on the inhibition of perioperative oral bacterial growth in oral cancer patients: a preliminary study : 抗菌薬局所投与による口腔がん術後患者の口腔内細菌数増殖抑制効果：予備的研究. (Thesis). Nagasaki University / 長崎大学. Retrieved from http://hdl.handle.net/10069/36834
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
船原, まどか. “Efficacy of topical antibiotic administration on the inhibition of perioperative oral bacterial growth in oral cancer patients: a preliminary study : 抗菌薬局所投与による口腔がん術後患者の口腔内細菌数増殖抑制効果：予備的研究.” 2016. Thesis, Nagasaki University / 長崎大学. Accessed April 17, 2021.
http://hdl.handle.net/10069/36834.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
船原, まどか. “Efficacy of topical antibiotic administration on the inhibition of perioperative oral bacterial growth in oral cancer patients: a preliminary study : 抗菌薬局所投与による口腔がん術後患者の口腔内細菌数増殖抑制効果：予備的研究.” 2016. Web. 17 Apr 2021.
Vancouver:
船原 . Efficacy of topical antibiotic administration on the inhibition of perioperative oral bacterial growth in oral cancer patients: a preliminary study : 抗菌薬局所投与による口腔がん術後患者の口腔内細菌数増殖抑制効果：予備的研究. [Internet] [Thesis]. Nagasaki University / 長崎大学; 2016. [cited 2021 Apr 17].
Available from: http://hdl.handle.net/10069/36834.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
船原 . Efficacy of topical antibiotic administration on the inhibition of perioperative oral bacterial growth in oral cancer patients: a preliminary study : 抗菌薬局所投与による口腔がん術後患者の口腔内細菌数増殖抑制効果：予備的研究. [Thesis]. Nagasaki University / 長崎大学; 2016. Available from: http://hdl.handle.net/10069/36834
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
5.
Nguyen, Darrène.
Influence de variations de conditions environnementales sur l'évolution des biofilms oraux : Influence of environnmental conditions variations on the evolution of oral biofilms.
Degree: Docteur es, Microbiologie-Immunologie, 2018, Bordeaux
URL: http://www.theses.fr/2018BORD0029
► L’écosystème buccal est un environnement complexe dans lequel cohabitent plus de 700 espèces de bactéries différentes. Un déséquilibre au sein de ce biofilm est à…
(more)
▼ L’écosystème buccal est un environnement complexe dans lequel cohabitent plus de 700 espèces de bactéries différentes. Un déséquilibre au sein de ce biofilm est à l’origine des principales maladies de la cavité buccale : les maladies carieuses et parodontales.Pendant plusieurs années, l’étude de l’écosystème buccal s’est faite par une approche réductionniste : les microbiologistes étudiaient les espèces bactériennes individuellement. Cette stratégie a permis d’examiner et de comprendre tous les différents composants de cet écosystème, sans pour autant pouvoir appliquer les conclusions de ces études au biofilm buccal dans son ensemble. En effet, les bactéries ne se comportent pas de la même façon lorsqu’elles sont à l’état planctonique, ou lorsqu’elles sont organisées en biofilm.La flore microbienne buccale est reconnue comme étant l’une des plus complexes dans le corps humain. La multitude d’espèces en présence complique l’étude de ce biofilm. En effet, sa reproduction in vitro est rendue difficile par la complexité des relations entre chacune des espèces. De plus, son recueil, et ses décomptes qualitatif et quantitatif restent très délicats.Dans la littérature sont décrits plusieurs modèles de biofilm.Les modèles pluri-espèces dynamiques in vitro ont l’avantage de se rapprocher des conditions retrouvées in vivo, permettant un certain flux de milieu, le contrôle de paramètres tels que le pH et la température, ainsi que l’élimination des déchets produits.Cependant, ces modèles restent très onéreux et difficiles de mise en place, ce qui complique l’étude du biofilm buccal. Egalement, l’identification des bactéries mises en présence reste un sujet d’étude délicat : en effet, les méthodes traditionnelles de culture montrent des limites, et ne permettent pas une analyse quantitative des résultats, essentielle à la compréhension des phénomènes mis en jeu dans cet écosystème.Le but de notre travail ici est la mise en place de modèle de biofilm pluri-espèces dynamique, fiable et reproductible, facile à mettre en place et moins onéreux que ceux décrits dans la littérature. Ce modèle de biofilm doit permettre l’étude de l’influence de variations de conditions environnementales sur ce dernier, ainsi que celle de candidats probiotiques ayant déjà prouvé leur efficacité sur des supports in vitro statiques. Enfin, toujours dans une optique de simplification, les différentes méthodes d’identification des biofilms formés sont comparées (méthodes de culture traditionnelle, PCR conventionnelle, spectrométrie de masse MALDI-TOF, et qPCR), afin d’établir un protocole d’identification reproductible permettant une analyse qualitative et quantitative des résultats.
The oral ecosystem presents a great complexity since it can harbor more than 700 different bacterial species. Most of them are organized in a biofilm on both the dental and the mucosal surfaces. Studying this complex environment is of utmost importance because a rupture in its stability can lead to the preeminence of pathogenic microorganisms, causing dental decay, gingivitis…
Advisors/Committee Members: Badet, Cécile (thesis director).
Subjects/Keywords: Biofilm; Modèle expérimental; Bactéries buccales; Biofilm; Experimental model; Oral bacteria
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APA ·
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MLA ·
Vancouver ·
CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Nguyen, D. (2018). Influence de variations de conditions environnementales sur l'évolution des biofilms oraux : Influence of environnmental conditions variations on the evolution of oral biofilms. (Doctoral Dissertation). Bordeaux. Retrieved from http://www.theses.fr/2018BORD0029
Chicago Manual of Style (16th Edition):
Nguyen, Darrène. “Influence de variations de conditions environnementales sur l'évolution des biofilms oraux : Influence of environnmental conditions variations on the evolution of oral biofilms.” 2018. Doctoral Dissertation, Bordeaux. Accessed April 17, 2021.
http://www.theses.fr/2018BORD0029.
MLA Handbook (7th Edition):
Nguyen, Darrène. “Influence de variations de conditions environnementales sur l'évolution des biofilms oraux : Influence of environnmental conditions variations on the evolution of oral biofilms.” 2018. Web. 17 Apr 2021.
Vancouver:
Nguyen D. Influence de variations de conditions environnementales sur l'évolution des biofilms oraux : Influence of environnmental conditions variations on the evolution of oral biofilms. [Internet] [Doctoral dissertation]. Bordeaux; 2018. [cited 2021 Apr 17].
Available from: http://www.theses.fr/2018BORD0029.
Council of Science Editors:
Nguyen D. Influence de variations de conditions environnementales sur l'évolution des biofilms oraux : Influence of environnmental conditions variations on the evolution of oral biofilms. [Doctoral Dissertation]. Bordeaux; 2018. Available from: http://www.theses.fr/2018BORD0029
Northeastern University
6.
Murugkar, Pallavi Pradeep.
Cultivating previously uncultured bacteria from the human oral cavity.
Degree: PhD, Department of Biology, 2013, Northeastern University
URL: http://hdl.handle.net/2047/d20004864
► Our knowledge of the bacteria that make up the human microbiome and the roles they play in health and disease is severely limited, and one…
(more)
▼ Our knowledge of the bacteria that make up the human microbiome and the roles they play in health and disease is severely limited, and one of the greatest causes of that limitation is the inability to culture many of these organisms. It is estimated that 50% of the human oral flora is uncultured (Aas et al., 2005) and the essential challenge is to develop methods for cultivating these elusive organisms, in order to understand the role of the oral microbiome in human health. It was previously discovered that many natural bacterial isolates from environments outside of the human body were uncultured due to their dependence on growth factors that are normally provided by other organisms in the environment (D'Onofrio et al., 2010). The hypothesis is that similar interactions are responsible for the failure to culture many of the organisms that make up the human microbiome. The goal of this study was to cultivate previously uncultured organisms from the oral cavity using co-culture techniques, identify their limiting growth factors and to determine the ubiquity of these growth factor-requiring organisms in the oral cavity. Several dependent bacteria from the oral cavity were isolated using co-culture techniques. One previously uncultured bacterium, KLE1280, was chosen as the model organism to identify its growth factor requirement. KLE1280 is related to Porphyromonas catoniae by 16S rRNA sequencing, and KLE1280 was isolated in co-culture with an oral isolate closely related to Staphylococcus hominis which acts as a helper. We found that this isolate (KLE1280) would also grow in the presence of E. coli, which allowed for the screening of a library of deletion mutants in search of a growth factor. E. coli mutants lacking menaquinone biosynthesis genes were unable to induce growth of Porphyromonas sp KLE1280. Exogenously added menaquinone 4 (MK4) induces growth of KLE1280. Along with MK4, heme (naturally occurring or synthetic) is also required for its growth. Based on these results, whole genome sequencing was done by our collaborator George Weinstock's group at The Genome Institute at Washington University. It was confirmed that this isolate is indeed missing the menaquinone biosynthesis genes. It appears to be very specific in its requirement for MK4, as KLE1280 was not induced by any quinone except MK4 or an intermediate of the menaquinone pathway, 1,4-dihydroxy-2-naphthoquinoic acid (DHNA). Two other species of Porphyromonas were also dependent on MK4. We hypothesize that other uncultured bacteria might be deficient in the same or similar growth factors, and similar to the model organism studied here, could be very specific in their growth factor requirement. It is therefore necessary to identify more growth factors in order to cultivate more organisms from the human microbiome. Using this approach may allow us to isolate many more uncultured organisms.
Subjects/Keywords: uncultered bacteria; oral microbiome; KLE1280; Bacteriology; Biology; Microbiology
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APA (6th Edition):
Murugkar, P. P. (2013). Cultivating previously uncultured bacteria from the human oral cavity. (Doctoral Dissertation). Northeastern University. Retrieved from http://hdl.handle.net/2047/d20004864
Chicago Manual of Style (16th Edition):
Murugkar, Pallavi Pradeep. “Cultivating previously uncultured bacteria from the human oral cavity.” 2013. Doctoral Dissertation, Northeastern University. Accessed April 17, 2021.
http://hdl.handle.net/2047/d20004864.
MLA Handbook (7th Edition):
Murugkar, Pallavi Pradeep. “Cultivating previously uncultured bacteria from the human oral cavity.” 2013. Web. 17 Apr 2021.
Vancouver:
Murugkar PP. Cultivating previously uncultured bacteria from the human oral cavity. [Internet] [Doctoral dissertation]. Northeastern University; 2013. [cited 2021 Apr 17].
Available from: http://hdl.handle.net/2047/d20004864.
Council of Science Editors:
Murugkar PP. Cultivating previously uncultured bacteria from the human oral cavity. [Doctoral Dissertation]. Northeastern University; 2013. Available from: http://hdl.handle.net/2047/d20004864
Wilfrid Laurier University
7.
Nechacov, Sidney.
Factors Affecting Biofilm Formation in Oral Pathogenic Bacteria of the Red Complex.
Degree: 2019, Wilfrid Laurier University
URL: https://scholars.wlu.ca/etd/2173
► Periodontal disease, also known as periodontitis, is a dental disease that is estimated to affect 2.4 billion people worldwide. This disease is characterized by chronic…
(more)
▼ Periodontal disease, also known as periodontitis, is a dental disease that is estimated to affect 2.4 billion people worldwide. This disease is characterized by chronic inflammation and bleeding of gums, as well as loss of tooth bone density. Currently, treatments for the disease include dental surgeries and various types of antibiotics, but few are targeted specifically at the Red Complex bacteria which are strongly associated with chronic periodontitis. The Red Complex consists of three bacteria: Tannerella forsythia, Porphyromonas gingivalis and Treponema denticola. Each of these bacteria, are anaerobic oral pathogens that reside in the subgingival pocket adjacent to alveolar bone. As part of the mechanism of pathogenesis, these bacteria generate biofilms that are matrices that provide protection from the host immune system and antibiotics, allowing nutrient sequestering and bacterial growth. These biofilms, in turn, are recognized and attacked by the host’s immune system leading to further progression of the disease due to increased inflammation. Since these bacterial biofilms play such a key role in the progression of periodontitis, it is important to understand how the bacteria generate biofilms both individually and together, in a multiorganismal biofilm. This research involved a novel approach in the co-culturing of the Red Complex bacteria in multiorganismal biofilms, statically, and then treating these bacteria and associated biofilms with various antimicrobials and anti-biofilm agents. It was established that the Red Complex multiorganismal biofilm consisted of predominantly P. gingivalis, followed by T. denticola and finally, T. forsythia statically and through molecular techniques. Treatment of Red Complex with sodium fluoride, sodium bicarbonate, hydrogen peroxide, sodium nitroprusside and Mirexus Compounds A, B and C statically, uncovered novel minimum inhibitory concentration (MIC) and minimum biofilm eradication concentration (MBEC) values, which could aid in developing novel preventative treatments for periodontal disease. The findings of this research indicated that, 100 µg/mL of sodium fluoride, 14 mg/mL of sodium bicarbonate and 0.05% (v/v) hydrogen peroxide, appeared to be both the MIC and MBEC values for treatment of the Red Complex. By quantifying the effects of the most effective treatments (100 µg/mL sodium fluoride, 14 mg/mL sodium bicarbonate and 0.075% (v/v) hydrogen peroxide) using molecular techniques, such as reverse-transcription quantitative-polymerase chain reaction (RT-qPCR) and denaturing gradient gel electrophoresis (DGGE), a deepened understanding of the shifts in the structural profile of the Red Complex multiorganismal biofilm was achieved. Ultimately, this research provided a more in depth understanding of the Red Complex bacteria, their growth and biofilm formation conditions and effective treatments that could be used to prevent the onset or progression of periodontal disease.
Subjects/Keywords: Microbiology; Pathogenic bacteria; Red Complex; Oral microbiome; Dysbiosis; Medical Microbiology
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APA (6th Edition):
Nechacov, S. (2019). Factors Affecting Biofilm Formation in Oral Pathogenic Bacteria of the Red Complex. (Thesis). Wilfrid Laurier University. Retrieved from https://scholars.wlu.ca/etd/2173
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Nechacov, Sidney. “Factors Affecting Biofilm Formation in Oral Pathogenic Bacteria of the Red Complex.” 2019. Thesis, Wilfrid Laurier University. Accessed April 17, 2021.
https://scholars.wlu.ca/etd/2173.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Nechacov, Sidney. “Factors Affecting Biofilm Formation in Oral Pathogenic Bacteria of the Red Complex.” 2019. Web. 17 Apr 2021.
Vancouver:
Nechacov S. Factors Affecting Biofilm Formation in Oral Pathogenic Bacteria of the Red Complex. [Internet] [Thesis]. Wilfrid Laurier University; 2019. [cited 2021 Apr 17].
Available from: https://scholars.wlu.ca/etd/2173.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Nechacov S. Factors Affecting Biofilm Formation in Oral Pathogenic Bacteria of the Red Complex. [Thesis]. Wilfrid Laurier University; 2019. Available from: https://scholars.wlu.ca/etd/2173
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
University of Pretoria
8.
Almansa Ruiz, Jose Carlos.
Bacterial
profiles and antibiograms of the
bacteria isolated of the exposed
pulps of dog and
cheetah canine teeth.
Degree: Companion Animal Clinical
Studies, 2012, University of Pretoria
URL: http://hdl.handle.net/2263/30685
► Objectives: The aims of this study was to investigate the RC microbiota in CCF canine teeth in the domestic dogs (Canis familiaris) and cheetahs (Acinonyx…
(more)
▼ Objectives: The aims of this study was to investigate the
RC microbiota in CCF canine teeth in the domestic dogs (Canis
familiaris) and cheetahs (Acinonyx jubatus), identify the possible
factors related to the presence of aerobic or anaerobic
bacteria
and evaluate and evaluate antibiotic susceptibility of
bacteria
isolated. Animals: Thirty nine animals suffering from CCF of their
canine teeth were included in this study, of which 20 were dogs and
19 were cheetahs. Procedures: Evaluation of the
oral cavity of
animals while under general anaesthesia was performed and those
without necrotic pulps or those that had received antibiotic
therapy in the previous two weeks were excluded. Microbial samples
were taken from 63 RC of which 27 were from dogs and 36 were from
cheetahs. Strict anaerobic and aerobic techniques were used in
parallel for plating, incubation and identification of the
bacteria
isolated in this manner. In an attempt to evaluate the sensitivity
of the culture media and anaerobic technique used, additional
samples were collected after the samples for bacterial isolation
had been taken from the last eight pulps. These comprised those
from six cheetahs and two dogs and were analysed using culture
techniques and an initial screening with the 16S rRNA-specific PCR.
Results: • Dogs: A total of 49 cultivable isolates were recovered
belonging to 19 different bacterial species and 13 different
genera. Individual RC yielded a maximum of four bacterial species.
Of the bacterial isolates, 4.08 % were strict anaerobes, being
represented by Clostridium acetobulitycum (2.04 %) and Prevotella
melalinogenica (2.04 % ). The incidence of aerobic
bacteria and
facultative anaerobic
bacteria in this study were 18.36 % and 77.56
%respectively of all the bacterial isolates. Of these Pasteurella
multocida ( 10.20 % ), Corynebacterium spp. (10.20 %), Moraxella
spp. (8.17 %), Bacillus spp. (6.12 %), Aeromonas salmonicida (6.12
%), Escherichia coli (6.12 %) and Pseudomonas aeruginosa (6.12 %)
were the
bacteria most frequently isolated. In summary, the RC
microflora was found to be predominantly Gram negative facultative
anaerobic microorganisms. The antibiotic agents that showed the
highest efficacy in vitro against the different
bacteria isolates
were Enrofloxacin (85.21 % ), Gentamicin (92.39 %), Chloramphenicol
(89.13 %). • Cheetahs: A total of 59 cultivable isolates, belonging
to 19 different microbial species and 13 different genera were
recovered from 36 RC sampled. Thirty-two (54.49 %) of the
cultivable isolates were Gram positive while 27 (45.71 %) were Gram
negative. Individual root canals each yielded a maximum of six
species. Four RC had no cultivable
bacteria. The bacterial micro
flora recovered from the RC of the animals showed a higher number
of facultative anaerobes (62.72 % of all the bacterial isolates).
Aerobic isolates were 28.81 %, and strict anaerobes 8.47 % of all
the isolates. The latter species comprised Clostridium sordelli
(5.08 % ), and Clostridium septicum (3.38 % ). The species with the
highest isolation…
Advisors/Committee Members: Steenkamp, Gerhardus (advisor), Bosman, Anna-Mari (coadvisor).
Subjects/Keywords: Bacteria; Canine
teeth; Cheetah
(Acinonyx jubatus);
Dogs; Oral
cavity;
Animals; UCTD
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APA ·
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Export
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Manager
APA (6th Edition):
Almansa Ruiz, J. C. (2012). Bacterial
profiles and antibiograms of the
bacteria isolated of the exposed
pulps of dog and
cheetah canine teeth. (Masters Thesis). University of Pretoria. Retrieved from http://hdl.handle.net/2263/30685
Chicago Manual of Style (16th Edition):
Almansa Ruiz, Jose Carlos. “Bacterial
profiles and antibiograms of the
bacteria isolated of the exposed
pulps of dog and
cheetah canine teeth.” 2012. Masters Thesis, University of Pretoria. Accessed April 17, 2021.
http://hdl.handle.net/2263/30685.
MLA Handbook (7th Edition):
Almansa Ruiz, Jose Carlos. “Bacterial
profiles and antibiograms of the
bacteria isolated of the exposed
pulps of dog and
cheetah canine teeth.” 2012. Web. 17 Apr 2021.
Vancouver:
Almansa Ruiz JC. Bacterial
profiles and antibiograms of the
bacteria isolated of the exposed
pulps of dog and
cheetah canine teeth. [Internet] [Masters thesis]. University of Pretoria; 2012. [cited 2021 Apr 17].
Available from: http://hdl.handle.net/2263/30685.
Council of Science Editors:
Almansa Ruiz JC. Bacterial
profiles and antibiograms of the
bacteria isolated of the exposed
pulps of dog and
cheetah canine teeth. [Masters Thesis]. University of Pretoria; 2012. Available from: http://hdl.handle.net/2263/30685
9.
Privitera, Salvatore.
Identificazione delle specie batteriche della placca subgengivale quale fattore di rischio per patologie parodontali in donne con variazioni ormonali fisiologiche e parafisiologiche.
Degree: 2014, Università degli Studi di Catania
URL: http://hdl.handle.net/10761/1564
► I principali obiettivi di questo studio sono stati quelli di valutare le implicazioni della menopausa sulla salute orale, nelle pazienti in trattamento con TOS (terapia…
(more)
▼ I principali obiettivi di questo studio sono stati quelli di valutare le implicazioni della menopausa sulla salute orale, nelle pazienti in trattamento con TOS (terapia ormonale sostitutiva) o meno, con particolare riferimento a modificazioni quali-quantitative dei principali batteri patogeni parodontali, che possono portare ad alterazioni dell equilibrio omeostatico del microbiota a favore di alcune specie più frequentemente coinvolte nell eziopatogenesi delle parodontopatie.
A tal fine, sono stati presi in considerazione gli effetti del trattamento ormonale sostitutivo (TOS) usando come termine di paragone il microbiota orale di donne in menopausa non sottoposte a TOS.
Sono stati esaminati 64 pazienti di età compresa fra i 45 e gli 80 anni (età media 59 anni) in menopausa clinicamente accertata, che accedevano presso l Istituto di Patologia Ostetrica e Ginecologica dell Azienda Ospedaliero-Universitaria Policlinico-Vittorio Emanuele G. Rodolico di Catania, in 60 dei quali è stata diagnosticata una parodontite cronica.
Su tali pazienti sono stati valutati i livelli ormonali, la densità ossea attraverso la MOC e le condizioni di igiene orale mediante la visita e la compilazione di uno specifico questionario anamnestico (ultima visita odontoiatrica effettuata, trattamenti odontoiatrici subiti nel corso degli anni).
Per ogni paziente sono stati eseguiti prelievi di placca sottogengivale in 4 siti, in prossimità del 1° e 2° molare, mediante coni di carta sterili o paper point.
In sede di visita sono stati raccolti i seguenti parametri clinici mediante l utilizzo di una sonda parodontale: sanguinamento al sondaggio (Bleeding On Probing, BOP), l indice di placca (Plaque Index, PI), la profondità della tasca parodontale (Probing Pocket Depth, PPD), la recessione gengivale (gingival recession, REC), la mobilità degli elementi dentali (MOB) e infine la presenza di essudato, come descritto da Community Periodontal Index of Treatment Needs (CPITN) [Ainamo J, et al. Development of the World Health Organization (WHO) Community periodontal index of treatment need (CPITN). Int Dent J 1982, 32:281-291.]
Per quanto concerne i parametri clinici parodontali analizzati, l'aumento dell indice di placca, della profondità della tasca parodontale e quindi delle recessioni gengivali e della mobilità dentale sono stati osservati con maggiore frequenza nei pazienti sottoposti a TOS.
La ragione di ciò è dovuta principalmente a una scarsa igiene orale e alle abitudini viziate (fumo, alimentazione errata, mancati controlli dal dentista).
Nonostante i programmi di prevenzione dentale e una migliorata igiene orale, da questo lavoro è emerso che, sebbene la parodontite cronica, sembrasse una patologia mantenuta sotto controllo, nel corso degli ultimi anni la frequenza dei pazienti affetti dalla suddetta patologia è in netto aumento.
Un dato importante lo si riscontra per l appunto nel soggetto femminile in menopausa, periodo, questo, caratterizzato da cambiamenti fisiologici importanti legati principalmente alla ridotta produzione di…
Subjects/Keywords: Area 06 - Scienze mediche; Periodontitis, menopause, oral health, periodontal bacteria
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APA ·
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APA (6th Edition):
Privitera, S. (2014). Identificazione delle specie batteriche della placca subgengivale quale fattore di rischio per patologie parodontali in donne con variazioni ormonali fisiologiche e parafisiologiche. (Thesis). Università degli Studi di Catania. Retrieved from http://hdl.handle.net/10761/1564
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Privitera, Salvatore. “Identificazione delle specie batteriche della placca subgengivale quale fattore di rischio per patologie parodontali in donne con variazioni ormonali fisiologiche e parafisiologiche.” 2014. Thesis, Università degli Studi di Catania. Accessed April 17, 2021.
http://hdl.handle.net/10761/1564.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Privitera, Salvatore. “Identificazione delle specie batteriche della placca subgengivale quale fattore di rischio per patologie parodontali in donne con variazioni ormonali fisiologiche e parafisiologiche.” 2014. Web. 17 Apr 2021.
Vancouver:
Privitera S. Identificazione delle specie batteriche della placca subgengivale quale fattore di rischio per patologie parodontali in donne con variazioni ormonali fisiologiche e parafisiologiche. [Internet] [Thesis]. Università degli Studi di Catania; 2014. [cited 2021 Apr 17].
Available from: http://hdl.handle.net/10761/1564.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Privitera S. Identificazione delle specie batteriche della placca subgengivale quale fattore di rischio per patologie parodontali in donne con variazioni ormonali fisiologiche e parafisiologiche. [Thesis]. Università degli Studi di Catania; 2014. Available from: http://hdl.handle.net/10761/1564
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Northeastern University
10.
Stancyk, David.
Competition between novel Streptococcus spp. from the human oral cavity.
Degree: MS, Department of Biology, 2014, Northeastern University
URL: http://hdl.handle.net/2047/d20005073
► Genomics studies have shown that the oral microbiome harbors a complex bacterial community and that many of its members have not been cultured. The bacteria…
(more)
▼ Genomics studies have shown that the oral microbiome harbors a complex bacterial community and that many of its members have not been cultured. The bacteria of the oral microbiome are known to form complex multispecies assemblages where species both compete and synergize to colonize each niche in the mouth. Understanding how the dynamics of this microbial ecosystem work could reveal how oral biofilm develops, how to control pathogenic bacteria or how bacterial communities transition from commensal to disease causing. Co-culturing multiple species together is a promising tool in microbial ecology as it may provide information missed by -omics studies or studies of monocultures. An earlier study (Sizova et al. 2012) used a combination of new and conventional culture techniques to generate a collection of oral bacteria that had not previously been cultured. This collection includes numerous strains of novel Streptococcus. This group of oral bacteria is of particular interest and importance. Many of the oral streptococci are potential pathogens that are implicated in oral, respiratory and cardiac disease (Kreth et al., 2009). They also interact and compete with each other as they colonize the dental surface (Donoghue & Tyler, 1975; Kreth, Zhang, & Herzberg, 2008). These interactions not only determine which strains are able to establish themselves within the niche, but also whether or not a community containing a pathogenic species becomes infectious or remains benign (Sbordone & Bortolaia, 2003). Our results show how phylogenetic distance, hydrogen peroxide and carbon dioxide are involved in the interspecies interactions of our novel Streptococcus spp. The combination of these factors in competition between Streptococcus provides insights into the mechanisms involved in bacterial interactions, and highlights the importance of volatile compounds in competition within microbial communities.
Subjects/Keywords: competition; human microbiome; hydrogen peroxide; microbial interactions; oral bacteria; Biology; Streptococcus; Mouth; Microbiology; Biofilms; Pathogenic bacteria; Competition (Biology)
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APA ·
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APA (6th Edition):
Stancyk, D. (2014). Competition between novel Streptococcus spp. from the human oral cavity. (Masters Thesis). Northeastern University. Retrieved from http://hdl.handle.net/2047/d20005073
Chicago Manual of Style (16th Edition):
Stancyk, David. “Competition between novel Streptococcus spp. from the human oral cavity.” 2014. Masters Thesis, Northeastern University. Accessed April 17, 2021.
http://hdl.handle.net/2047/d20005073.
MLA Handbook (7th Edition):
Stancyk, David. “Competition between novel Streptococcus spp. from the human oral cavity.” 2014. Web. 17 Apr 2021.
Vancouver:
Stancyk D. Competition between novel Streptococcus spp. from the human oral cavity. [Internet] [Masters thesis]. Northeastern University; 2014. [cited 2021 Apr 17].
Available from: http://hdl.handle.net/2047/d20005073.
Council of Science Editors:
Stancyk D. Competition between novel Streptococcus spp. from the human oral cavity. [Masters Thesis]. Northeastern University; 2014. Available from: http://hdl.handle.net/2047/d20005073
Northeastern University
11.
Chilaka, Amanda Chinyere.
Genomic analysis of five novel, formerly-uncultivable oral bacteria belonging to the family Peptostreptococcaceae.
Degree: MS, Department of Biology, 2014, Northeastern University
URL: http://hdl.handle.net/2047/d20005091
► According to the Human Oral Microbiome Database, approximately 700 prokaryotic species are present in the human oral cavity; 49% of these are cultivable and officially…
(more)
▼ According to the Human Oral Microbiome Database, approximately 700 prokaryotic species are present in the human oral cavity; 49% of these are cultivable and officially named, 17% are cultivable and unnamed, and 34% are a part of uncultivated phylotypes (Chen et al., 2010; Dewhirst et al., 2010). Cultivation of oral bacteria is important because a better understanding of their physiology could help in treating such pathologies as periodontal disease; between 2009 and 2010, approximately 47.2% of adults age 30 and up had periodontitis (Thornton-Evans et al., 2013). In our laboratory, five oral bacteria, that were formerly classified as uncultivable, were cultivated from anaerobic enrichments from the subgingival cavities of healthy human subjects as part of the Human Microbiome Project (Sizova et al., 2012). Isolates were originally designated as Eubacteriaceae bacterium ACC19a, CM2, CM5, OBRC8 and Eubacterium sp. AS15. They have since been renamed as Peptostreptococcaceae spp. ACC19a, CM2, CM5, OBRC8, and AS15. The first three isolates were sequenced at the BROAD Institute and the latter two at the JCVI.; It is hypothesized that a combination of annotation pipelines will provide a broader representation of genes present in each bacterial genome and could reveal metabolic pathways that could be missed. Supplemental tools such the Rapid Annotation using Subsystem Technology (RAST) service (Aziz et al., 2008) and Pathway Tools (Karp, Paley, Krummenacker, & al, 2010) were invaluable in determining some of the metabolic pathways that are present or absent for the isolates. One of the aims of this project is to determine what differences there are between the oral isolates and their closest relatives, as well as determining the differences between the isolates.
Subjects/Keywords: Human Microbiome Project; oral bacteria; Peptostreptococcaceae; Biology; Streptococcus; Eubacteriales; Prokaryotes; Bacterial genomes
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APA ·
Chicago ·
MLA ·
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CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Chilaka, A. C. (2014). Genomic analysis of five novel, formerly-uncultivable oral bacteria belonging to the family Peptostreptococcaceae. (Masters Thesis). Northeastern University. Retrieved from http://hdl.handle.net/2047/d20005091
Chicago Manual of Style (16th Edition):
Chilaka, Amanda Chinyere. “Genomic analysis of five novel, formerly-uncultivable oral bacteria belonging to the family Peptostreptococcaceae.” 2014. Masters Thesis, Northeastern University. Accessed April 17, 2021.
http://hdl.handle.net/2047/d20005091.
MLA Handbook (7th Edition):
Chilaka, Amanda Chinyere. “Genomic analysis of five novel, formerly-uncultivable oral bacteria belonging to the family Peptostreptococcaceae.” 2014. Web. 17 Apr 2021.
Vancouver:
Chilaka AC. Genomic analysis of five novel, formerly-uncultivable oral bacteria belonging to the family Peptostreptococcaceae. [Internet] [Masters thesis]. Northeastern University; 2014. [cited 2021 Apr 17].
Available from: http://hdl.handle.net/2047/d20005091.
Council of Science Editors:
Chilaka AC. Genomic analysis of five novel, formerly-uncultivable oral bacteria belonging to the family Peptostreptococcaceae. [Masters Thesis]. Northeastern University; 2014. Available from: http://hdl.handle.net/2047/d20005091
12.
Monteiro, Julie Marie Martins.
Avaliação da atividade antimicrobiana e citotóxica da biflorina frente a micro-organismos orais e a uma linhagem de célula cancerígena da cavidade bucal.
Degree: 2015, Universidade Federal do Amazonas
URL: http://tede.ufam.edu.br/handle/tede/5015
► Investigações fitoquímicas levaram ao isolamento e caracterização da Biflorina que dentre os compostos já isolados da Capraria biflora, mostrou-se promissora com uma potente atividade antimicrobiana…
(more)
▼ Investigações fitoquímicas levaram ao isolamento e caracterização da Biflorina que dentre os
compostos já isolados da Capraria biflora, mostrou-se promissora com uma potente atividade
antimicrobiana e antitumoral. Portanto, o objetivo deste estudo foi avaliar a atividade
antimicrobiana da biflorina sobre micro-organismos da cavidade bucal e atividade citotóxica
sobre linhagem de célula cancerígena da cavidade bucal. A triagem da atividade
antimicrobiana da biflorina foi realizada através da técnica de difusão em ágar com os microorganismos
Streptococcus mutans ATCC25175, Streptococcus salivarius ATCC7073,
Streptococcus oralis ATCC10557 e Lactobacillus paracasei ATCC335. A quantificação desta
atividade antimicrobiana foi realizada pela técnica de microdiluição em caldo para
determinação da concentração inibitória mínima (CIM). Para avaliação da citotoxicidade
sobre linhagem cancerígena da cavidade bucal foi utilizada a linhagem celular CAL-27 e o
ensaio de Alamar blue. No teste de difusão em ágar, a biflorina apresentou atividade
antimicrobiana frente atodos os micro-organismos avaliados. A maior atividade
antimicrobiana foi observada nos ensaios com o Lactobacillus paracasei e a menor nos
ensaios com Streptococcus salivarius. Considerando que a biflorina foi ativa frente a todos os
micro-organismos testados, determinou-se a concentração inibitória mínima. No teste de
microdiluição a biflorina apresentou menor CIM frente ao Streptococcus mutans (MIC=0,70
μg/mL) e Streptococcus salivarius (MIC 3,125 μg/mL). Enquanto a clorexidina a 2% foi
eficaz em sua menor diluição (0,015%) sobre todas as bactérias testadas. No teste de
citotoxicidade do Alamar blue, o valor da IC50 da Biflorina sobre a CAL-27 em 72 horas foi
de 3.69 (3.17 – 4.30μM/mL) e a Doxorrubicina (controle) foi 0.039 (0.036-0.088μg/mL). Os
resultados sugerem que a biflorina pode ser incorporada a produtos de uso odontológico e
para o tratamento de neoplasias da cavidade oral, porém é necessário dar continuidade aos
estudos com esta substância nesta área
Phytochemical investigations led to the isolation and characterization of biflorin that among
the compounds already isolated from Capraria biflora, showed promise with a powerful
antimicrobial and antitumor activity. Therefore, the aim of this study was to evaluate the
antimicrobial activity of biflorin on the oral cavity microorganisms and cytotoxic activity on
cancer cell lineage of the oral cavity. The screening of antimicrobial activity biflorin was
performed by diffusion technique in agar with the microorganisms Streptococcus mutans
ATCC25175, Streptococcus salivarius ATCC7073, Streptococcus oralis ATCC10557 and
Lactobacillus paracasei ATCC335. The quantification of the antimicrobial activity was
performed by broth microdilution technique to determine the minimum inhibitory
concentration (MIC). To evaluate the cytotoxicity of cancer lineage of the oral cavity was
used cell line CAL-27 and the Alamar blue assay. In the agar diffusion test, biflorin presented
…
Advisors/Committee Members: Magalhães, Karen Regina Carim da Costa, 64368238249, http://lattes.cnpq.br/4015149205638769.
Subjects/Keywords: Atividade antimicrobiana; Atividade citotóxica; Bactérias orais; Antimicrobial activity; Cytotoxicity; Oral bacteria; CIÊNCIAS DA SAÚDE: ODONTOLOGIA
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APA (6th Edition):
Monteiro, J. M. M. (2015). Avaliação da atividade antimicrobiana e citotóxica da biflorina frente a micro-organismos orais e a uma linhagem de célula cancerígena da cavidade bucal. (Masters Thesis). Universidade Federal do Amazonas. Retrieved from http://tede.ufam.edu.br/handle/tede/5015
Chicago Manual of Style (16th Edition):
Monteiro, Julie Marie Martins. “Avaliação da atividade antimicrobiana e citotóxica da biflorina frente a micro-organismos orais e a uma linhagem de célula cancerígena da cavidade bucal.” 2015. Masters Thesis, Universidade Federal do Amazonas. Accessed April 17, 2021.
http://tede.ufam.edu.br/handle/tede/5015.
MLA Handbook (7th Edition):
Monteiro, Julie Marie Martins. “Avaliação da atividade antimicrobiana e citotóxica da biflorina frente a micro-organismos orais e a uma linhagem de célula cancerígena da cavidade bucal.” 2015. Web. 17 Apr 2021.
Vancouver:
Monteiro JMM. Avaliação da atividade antimicrobiana e citotóxica da biflorina frente a micro-organismos orais e a uma linhagem de célula cancerígena da cavidade bucal. [Internet] [Masters thesis]. Universidade Federal do Amazonas; 2015. [cited 2021 Apr 17].
Available from: http://tede.ufam.edu.br/handle/tede/5015.
Council of Science Editors:
Monteiro JMM. Avaliação da atividade antimicrobiana e citotóxica da biflorina frente a micro-organismos orais e a uma linhagem de célula cancerígena da cavidade bucal. [Masters Thesis]. Universidade Federal do Amazonas; 2015. Available from: http://tede.ufam.edu.br/handle/tede/5015
University of Melbourne
13.
HEATH, JACQUELINE.
Characterisation of the function of PG1058 in Porphyromonas gingivalis.
Degree: 2016, University of Melbourne
URL: http://hdl.handle.net/11343/91557
► Porphyromonas gingivalis utilises the Bacteroidetes-specific Type IX Secretion System (T9SS) to export proteins across the outer membrane (OM), including virulence factors such as the gingipains.…
(more)
▼ Porphyromonas gingivalis utilises the Bacteroidetes-specific Type IX Secretion System (T9SS) to export proteins across the outer membrane (OM), including virulence factors such as the gingipains. The secreted proteins have a conserved carboxy-terminal domain essential for type IX secretion that is cleaved upon export. In P. gingivalis the T9SS substrates undergo post-translational modification with anionic lipopolysaccharide (A-LPS) and are attached to the OM. Comparative analyses of several Bacteroidetes genomes identified PG1058 as a candidate for a novel component of the T9SS. Inactivation of pg1058 in P. gingivalis resulted in slowed growth, loss of colonial pigmentation and surface-associated gingipain activity, a phenotype common to T9SS mutants.
This study revealed normal cell architecture and no difference in OM solute permeability between the wild-type and pg1058- mutant. Thus the mutant phenotype was unlikely due to structural effects on cell division or perturbation of OM integrity causing aberrant assembly and function of the T9SS.
T9SS substrates accumulated within the pg1058- mutant periplasm indicated perturbed T9SS function and the Kgp gingipain was shown to be absent from the cell surface, whilst A-LPS was present. This indicated that PG1058 is crucial for export of T9SS substrates but not for the export and surface-association of A-LPS.
PG1058 was localised as an OM-associated periplasmic protein. Several proteins crucial to the T9SS and modification processes have been identified in the OM including PorT, PorV and PorU the CTD peptidase, that is itself a substrate of the T9SS. Increased abundance of PorT and PorV was seen in the pg1058- mutant, along with several other T9SS components. PorU accumulated in the periplasm with other T9SS substrates. The localisation and abundance of PG1058 in porV-, porT- and porU- mutants was not grossly altered.
Bioinformatic analyses revealed that numerous Bacteroidetes possess multiple PG1058 homologues which correlated with multiple homologues of PorP, a known OM-associated T9SS component. In F. johnsoniae the PorP and PG1058 homologues were found adjacent in the genome suggesting a potential functional relationship between these proteins, with a role in substrate recognition and sorting being proposed.
Several T9SS substrates are involved in host cell interactions and dysregulation of the host immune response. Susceptibility to macrophage phagocytosis, ability to bind to oral epithelial cells, ability to haemagglutinate red blood cells and ability to co-aggregate with the oral pathogen Treponema denticola were reduced but not abolished in the pg1058- and porV- T9SS mutants. Combined with a largely equivalent pro-inflammatory cytokine response, this suggests that P. gingivalis possesses surface-associated molecules in addition to the T9SS substrates which can influence human host interactions and thereby the virulence of P. gingivalis. Subtle differences in the response to the pg1058- and porV- mutants suggest that there are differences between…
Subjects/Keywords: periodontitis; oral bacteria; Porphyromonas gingivalis; Bacteroidetes; Type IX Secretion System; PG1058; gingipains
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Export
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APA (6th Edition):
HEATH, J. (2016). Characterisation of the function of PG1058 in Porphyromonas gingivalis. (Doctoral Dissertation). University of Melbourne. Retrieved from http://hdl.handle.net/11343/91557
Chicago Manual of Style (16th Edition):
HEATH, JACQUELINE. “Characterisation of the function of PG1058 in Porphyromonas gingivalis.” 2016. Doctoral Dissertation, University of Melbourne. Accessed April 17, 2021.
http://hdl.handle.net/11343/91557.
MLA Handbook (7th Edition):
HEATH, JACQUELINE. “Characterisation of the function of PG1058 in Porphyromonas gingivalis.” 2016. Web. 17 Apr 2021.
Vancouver:
HEATH J. Characterisation of the function of PG1058 in Porphyromonas gingivalis. [Internet] [Doctoral dissertation]. University of Melbourne; 2016. [cited 2021 Apr 17].
Available from: http://hdl.handle.net/11343/91557.
Council of Science Editors:
HEATH J. Characterisation of the function of PG1058 in Porphyromonas gingivalis. [Doctoral Dissertation]. University of Melbourne; 2016. Available from: http://hdl.handle.net/11343/91557
14.
Monteiro, Julie Marie Martins.
Avaliação da atividade antimicrobiana e citotóxica da biflorina frente a micro-organismos orais e a uma linhagem de célula cancerígena da cavidade bucal.
Degree: 2015, Universidade Federal do Amazonas; Programa de Pós-graduação em Odontologia; UFAM; Brasil; Faculdade de Odontologia
URL: http://tede.ufam.edu.br/handle/tede/5015
► Submitted by Divisão de Documentação/BC Biblioteca Central ([email protected]) on 2016-06-08T20:01:21Z No. of bitstreams: 1 Dissertação - Julie Marie Martins Monteiro.pdf: 996366 bytes, checksum: 7736bdc6fce423bd155ddb42d5bb7df9 (MD5)…
(more)
▼ Submitted by Divisão de Documentação/BC Biblioteca Central ([email protected]) on 2016-06-08T20:01:21Z No. of bitstreams: 1 Dissertação - Julie Marie Martins Monteiro.pdf: 996366 bytes, checksum: 7736bdc6fce423bd155ddb42d5bb7df9 (MD5)
Approved for entry into archive by Divisão de Documentação/BC Biblioteca Central ([email protected]) on 2016-06-08T20:01:34Z (GMT) No. of bitstreams: 1 Dissertação - Julie Marie Martins Monteiro.pdf: 996366 bytes, checksum: 7736bdc6fce423bd155ddb42d5bb7df9 (MD5)
Approved for entry into archive by Divisão de Documentação/BC Biblioteca Central ([email protected]) on 2016-06-08T20:03:17Z (GMT) No. of bitstreams: 1 Dissertação - Julie Marie Martins Monteiro.pdf: 996366 bytes, checksum: 7736bdc6fce423bd155ddb42d5bb7df9 (MD5)
Made available in DSpace on 2016-06-08T20:03:17Z (GMT). No. of bitstreams: 1 Dissertação - Julie Marie Martins Monteiro.pdf: 996366 bytes, checksum: 7736bdc6fce423bd155ddb42d5bb7df9 (MD5)
Previous issue date: 2015-05-29
FAPEAM - Fundação de Amparo à Pesquisa do Estado do Amazonas
Phytochemical investigations led to the isolation and characterization of biflorin that among the compounds already isolated from Capraria biflora, showed promise with a powerful antimicrobial and antitumor activity. Therefore, the aim of this study was to evaluate the antimicrobial activity of biflorin on the oral cavity microorganisms and cytotoxic activity on cancer cell lineage of the oral cavity. The screening of antimicrobial activity biflorin was performed by diffusion technique in agar with the microorganisms Streptococcus mutans ATCC25175, Streptococcus salivarius ATCC7073, Streptococcus oralis ATCC10557 and Lactobacillus paracasei ATCC335. The quantification of the antimicrobial activity was performed by broth microdilution technique to determine the minimum inhibitory concentration (MIC). To evaluate the cytotoxicity of cancer lineage of the oral cavity was used cell line
CAL-27 and the Alamar blue assay. In the agar diffusion test, biflorin presented antimicrobial activity against all evaluated microorganisms. The greatest antimicrobial activity was observed in trials with Lactobacillus paracasei and lower in trials with Streptococcus salivarius. Whereas biflorin was active against all tested micro-organisms, it determined the minimum inhibitory concentration. In the microdilution test biflorin showed lower MIC against Streptococcus mutans (MIC = 0,70μg/mL) and Streptococcus salivarius (MIC 3,125 μg/mL). While the 2% chlorhexidine was effective in its lower dilution (0,015%) of all tested bacteria. In Alamar blue cytotoxicity assay, the IC50 value of biflorin on the CAL 27 in 72 hours was 3.69 (3.17 to 4.30μM/mL) and Doxorubicin (control) was 0.039 (0.036- 0.088 μM/mL).The results suggest that biflorin can be incorporated in dental applications and products for the treatment of cancers of the oral cavity, but it is necessary to continue the research
in this area with this substance
Investigações fitoquímicas levaram ao isolamento e caracterização…
Advisors/Committee Members: Magalhães, Karen Regina Carim da Costa.
Subjects/Keywords: Atividade antimicrobiana; Atividade citotóxica; Bactérias orais; Antimicrobial activity; Cytotoxicity; Oral bacteria; CIÊNCIAS DA SAÚDE: ODONTOLOGIA
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APA ·
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Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Monteiro, J. M. M. (2015). Avaliação da atividade antimicrobiana e citotóxica da biflorina frente a micro-organismos orais e a uma linhagem de célula cancerígena da cavidade bucal. (Masters Thesis). Universidade Federal do Amazonas; Programa de Pós-graduação em Odontologia; UFAM; Brasil; Faculdade de Odontologia. Retrieved from http://tede.ufam.edu.br/handle/tede/5015
Chicago Manual of Style (16th Edition):
Monteiro, Julie Marie Martins. “Avaliação da atividade antimicrobiana e citotóxica da biflorina frente a micro-organismos orais e a uma linhagem de célula cancerígena da cavidade bucal.” 2015. Masters Thesis, Universidade Federal do Amazonas; Programa de Pós-graduação em Odontologia; UFAM; Brasil; Faculdade de Odontologia. Accessed April 17, 2021.
http://tede.ufam.edu.br/handle/tede/5015.
MLA Handbook (7th Edition):
Monteiro, Julie Marie Martins. “Avaliação da atividade antimicrobiana e citotóxica da biflorina frente a micro-organismos orais e a uma linhagem de célula cancerígena da cavidade bucal.” 2015. Web. 17 Apr 2021.
Vancouver:
Monteiro JMM. Avaliação da atividade antimicrobiana e citotóxica da biflorina frente a micro-organismos orais e a uma linhagem de célula cancerígena da cavidade bucal. [Internet] [Masters thesis]. Universidade Federal do Amazonas; Programa de Pós-graduação em Odontologia; UFAM; Brasil; Faculdade de Odontologia; 2015. [cited 2021 Apr 17].
Available from: http://tede.ufam.edu.br/handle/tede/5015.
Council of Science Editors:
Monteiro JMM. Avaliação da atividade antimicrobiana e citotóxica da biflorina frente a micro-organismos orais e a uma linhagem de célula cancerígena da cavidade bucal. [Masters Thesis]. Universidade Federal do Amazonas; Programa de Pós-graduação em Odontologia; UFAM; Brasil; Faculdade de Odontologia; 2015. Available from: http://tede.ufam.edu.br/handle/tede/5015
University of Southern Mississippi
15.
Davis, Krystyn Elizabeth.
Effects of Prebiotics on Gut Bacterial Communities and Healing of Induced Colitis in Mice.
Degree: MS, Biological Sciences, 2016, University of Southern Mississippi
URL: https://aquila.usm.edu/masters_theses/194
► Inflammatory Bowel Diseases (IBD) cause chronic inflammation of the gastrointestinal tract and debilitating symptoms in those suffering from the diseases. After inducing colitis in…
(more)
▼ Inflammatory Bowel Diseases (IBD) cause chronic inflammation of the gastrointestinal tract and debilitating symptoms in those suffering from the diseases. After inducing colitis in a mouse model using Dextran Sulfate Sodium (DSS), prebiotics inulin and oligofructose enriched inulin (OEI) were used as treatments to determine their effects on the gut microbial community, physiological healing process, and immune response in the mice after initial inflammation and before subsequent inflammation, or relapse. The treatment with inulin led to an increase in regulatory T cell number, but this increase was not as significant as the increase induced by the OEI. Inulin increased the inflammation in the mouse colon, whereas inflammation was decreased in the colons of the mice treated with OEI. A three percent increase in butyrate producing
bacteria,
Clostridium cluster XIVa spp., was observed in mice treated with OEI before the relapse period when compared to untreated mice with colitis. The proposed mechanism for how the OEI led to decreased inflammation in the colons of the treated mice was that the introduction of the prebiotic allowed for an increase in butyrate producing
Clostridium cluster XIVa spp., which led to a direct increase in butyrate production in the colon. In turn, this butyrate production led to an increase in differentiation of regulatory T cells and an overall reduction of the immune response and inflammation in the mice treated with OEI. This reduction of immune response and inflammation allowed the mice that were treated with OEI to be more resistant to induced relapse.
Advisors/Committee Members: Shiao Wang, Fengwei Bai, Hao Xu.
Subjects/Keywords: Inflammatory Bowel Disease; colitis; prebiotics; gut microbiota; regulatory T cells; gut bacteria; Alternative and Complementary Medicine; Bacteria; Digestive, Oral, and Skin Physiology; Digestive System; Digestive System Diseases; Medical Immunology
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APA ·
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Export
to Zotero / EndNote / Reference
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APA (6th Edition):
Davis, K. E. (2016). Effects of Prebiotics on Gut Bacterial Communities and Healing of Induced Colitis in Mice. (Masters Thesis). University of Southern Mississippi. Retrieved from https://aquila.usm.edu/masters_theses/194
Chicago Manual of Style (16th Edition):
Davis, Krystyn Elizabeth. “Effects of Prebiotics on Gut Bacterial Communities and Healing of Induced Colitis in Mice.” 2016. Masters Thesis, University of Southern Mississippi. Accessed April 17, 2021.
https://aquila.usm.edu/masters_theses/194.
MLA Handbook (7th Edition):
Davis, Krystyn Elizabeth. “Effects of Prebiotics on Gut Bacterial Communities and Healing of Induced Colitis in Mice.” 2016. Web. 17 Apr 2021.
Vancouver:
Davis KE. Effects of Prebiotics on Gut Bacterial Communities and Healing of Induced Colitis in Mice. [Internet] [Masters thesis]. University of Southern Mississippi; 2016. [cited 2021 Apr 17].
Available from: https://aquila.usm.edu/masters_theses/194.
Council of Science Editors:
Davis KE. Effects of Prebiotics on Gut Bacterial Communities and Healing of Induced Colitis in Mice. [Masters Thesis]. University of Southern Mississippi; 2016. Available from: https://aquila.usm.edu/masters_theses/194
16.
Liza Barreto Vieira.
Eficácia do bochecho de quitosana a 0,4% sobre o biofilme e bactérias orais.
Degree: 2005, Universidade Federal do Rio Grande do Norte
URL: http://bdtd.bczm.ufrn.br/tedesimplificado//tde_busca/arquivo.php?codArquivo=66
► O objetivo deste estudo foi avaliar a eficácia do bochecho de quitosana a 0,4% com alto peso molecular e alto grau de desacetilização sobre a…
(more)
▼ O objetivo deste estudo foi avaliar a eficácia do bochecho de quitosana a 0,4% com alto peso molecular e alto grau de desacetilização sobre a redução do total de estreptococcus, Streptococcus mutans, total de lactobacilos e sobre os índices de placa visível e sangramento gengival. Para tanto, foram selecionados 68 estudantes saudáveis, com idade entre 11 e 13 anos, não alérgicos a crustáceos e que não tivessem usado antibiótico ou antimicrobiano nos últimos três meses ou durante o tratamento. Trinta e dois indivíduos utilizaram o bochecho teste e trinta e seis o controle. Os participantes bochecho 10 ML das soluções duas vezes ao dia, um pela manhã (supervisionado) e outro à tarde (não supervisionado, durante quinza dias. A coleta de saliva para análise microbiológica, bem como a aferição dos índices de placa visível e de sangramento gengival, deu-se antes do uso dos bochechos (linha base),no dia imediatamento após o último bochecho (tempo zero) e quinze dias após (tempo quinze). Esses dados foram coletados na escola e a saliva transportada em gelo até o laboratório. As amostras de saliva foram diluidas e 0,1ML da diluição 10 elevado a menos 1 foi semeada em Rogosa SL ágar para a posterior análise do total de lactobacilos; 0,1mL da diluição 10 elevado a menos 4 em Mitis Salivarus com bacitracina, para análise de S mutans, e 0,1mL da diluição 10 elevado a menos 6 em Mitis Salivarius para análise do total de estreptococous. As placas de Rogosa SL ágar foram incubadas em aerobiose a 37 grau centígrado por 72 horas e as de MSB e MS foram incubadas em anaeribiose em jarras Gaspak a 337 grau centígrado, por 48 horas, para posterior contagem das unidades formadoras de colônias (UFCs). O ensaio foi feito em duplicadta para cada grupo bacteriano analisado. O número de UFCs transformados em LOG10 foi analisado mediante os seguintes testes: ANOVA, t de Student emparelhado e não emparelhado, Friedman, Man-Whitney e teste do qui-quadrado. Na linha base, todas as variáveis analisadas no estudo foram semelhantes nos dois grupos testados. Em ambos os grupos, para o total de estreptococcus não houve diferença significativa ao longo do tempo; para o S. mutans, houve aumento estatisticamente significativo das UFCs da linha base para o T0. Para o total de lactobacilos, não houve diferença no grupo teste ao longo do tempo e, no controle, houve aumento significativo das UFCs da linha base para o T0. Em ambos os grupos, houve diminuição significativa do IPV ao longo do tempo. O ISG também apresentou redução ao longo do tempo, porém não foi significativa. Portanto, este estudo concluiu que o bochecho de quitosana a 0,4% não foi eficaz na redução das UFCs dos três grupos bacterianos analisados, assim como, na redução do IPV e ISG
The purpose of this study was evaluate the effectiveness of the chitosan at 0.4 with high molecular weight and high deacetylation degree mouthrinse over the total decrease of the streptococci, Streptococcus mutans, lactobaci/li and over the perceptible bacterial film and gingival bleeding indices. For that, a…
Advisors/Committee Members: Kenio Costa de Lima, Milton de Uzeda, Maria Ângela Fernandes Ferreira.
Subjects/Keywords: Agente antimicrobiano; Bochecho; Quitosana; ODONTOLOGIA SOCIAL E PREVENTIVA; Mouthrinse; Chitosan; Antimicrobial agent; Oral bacteria; Biofilm; Bactérias orais e biofilme
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APA ·
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to Zotero / EndNote / Reference
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APA (6th Edition):
Vieira, L. B. (2005). Eficácia do bochecho de quitosana a 0,4% sobre o biofilme e bactérias orais. (Thesis). Universidade Federal do Rio Grande do Norte. Retrieved from http://bdtd.bczm.ufrn.br/tedesimplificado//tde_busca/arquivo.php?codArquivo=66
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Vieira, Liza Barreto. “Eficácia do bochecho de quitosana a 0,4% sobre o biofilme e bactérias orais.” 2005. Thesis, Universidade Federal do Rio Grande do Norte. Accessed April 17, 2021.
http://bdtd.bczm.ufrn.br/tedesimplificado//tde_busca/arquivo.php?codArquivo=66.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Vieira, Liza Barreto. “Eficácia do bochecho de quitosana a 0,4% sobre o biofilme e bactérias orais.” 2005. Web. 17 Apr 2021.
Vancouver:
Vieira LB. Eficácia do bochecho de quitosana a 0,4% sobre o biofilme e bactérias orais. [Internet] [Thesis]. Universidade Federal do Rio Grande do Norte; 2005. [cited 2021 Apr 17].
Available from: http://bdtd.bczm.ufrn.br/tedesimplificado//tde_busca/arquivo.php?codArquivo=66.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Vieira LB. Eficácia do bochecho de quitosana a 0,4% sobre o biofilme e bactérias orais. [Thesis]. Universidade Federal do Rio Grande do Norte; 2005. Available from: http://bdtd.bczm.ufrn.br/tedesimplificado//tde_busca/arquivo.php?codArquivo=66
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Colorado State University
17.
Kemp, Jeffrey M.
Mucosal immunization of mice with a recombinant Salmonella choleraesuis that expresses a multimeric gonadotropin releasing hormone fusion protein.
Degree: PhD, Biomedical Sciences, 2011, Colorado State University
URL: http://hdl.handle.net/10217/70445
To view the abstract, please see the full text of the document.
Advisors/Committee Members: Graham, James (advisor), Huyvaert, Kathryn P. (committee member), Bowen, Richard (committee member), Miller, Lowell (committee member), Rhyan, Jack (committee member).Subjects/Keywords: immunocontraception; vaccine stabilization; recombinant bacteria; oral delivery
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APA ·
Chicago ·
MLA ·
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to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Kemp, J. M. (2011). Mucosal immunization of mice with a recombinant Salmonella choleraesuis that expresses a multimeric gonadotropin releasing hormone fusion protein. (Doctoral Dissertation). Colorado State University. Retrieved from http://hdl.handle.net/10217/70445
Chicago Manual of Style (16th Edition):
Kemp, Jeffrey M. “Mucosal immunization of mice with a recombinant Salmonella choleraesuis that expresses a multimeric gonadotropin releasing hormone fusion protein.” 2011. Doctoral Dissertation, Colorado State University. Accessed April 17, 2021.
http://hdl.handle.net/10217/70445.
MLA Handbook (7th Edition):
Kemp, Jeffrey M. “Mucosal immunization of mice with a recombinant Salmonella choleraesuis that expresses a multimeric gonadotropin releasing hormone fusion protein.” 2011. Web. 17 Apr 2021.
Vancouver:
Kemp JM. Mucosal immunization of mice with a recombinant Salmonella choleraesuis that expresses a multimeric gonadotropin releasing hormone fusion protein. [Internet] [Doctoral dissertation]. Colorado State University; 2011. [cited 2021 Apr 17].
Available from: http://hdl.handle.net/10217/70445.
Council of Science Editors:
Kemp JM. Mucosal immunization of mice with a recombinant Salmonella choleraesuis that expresses a multimeric gonadotropin releasing hormone fusion protein. [Doctoral Dissertation]. Colorado State University; 2011. Available from: http://hdl.handle.net/10217/70445
18.
Sogodogo, Elisabeth.
Détection des méthanogènes dans la flore oro-sinusale : Detection of methanogens in the oro-sinus flora.
Degree: Docteur es, Maladies infectieuses, 2019, Aix Marseille Université
URL: http://www.theses.fr/2019AIXM0238
► Dans la première partie de notre Thèse, nous avons revu la littérature sur les méthanogènes retrouvés dans les différents microbiotes de l’Homme. Nous avons également…
(more)
▼ Dans la première partie de notre Thèse, nous avons revu la littérature sur les méthanogènes retrouvés dans les différents microbiotes de l’Homme. Nous avons également fait un point sur les diverses méthodes utilisées pour la détection de ces microorganismes au laboratoire de microbiologie clinique.Dans une seconde partie de notre travail de Thèse, nous avons testé la production d’hydrogène par les bactéries anaérobies facultatives d’intérêt clinique, plus particulièrement les bactéries précédemment retrouvées associées avec les méthanogènes dans les échantillons cliniques. Nous avons observé que toutes les bactéries anaérobies facultatives n’ont pas la capacité de produire de l’hydrogène gazeux et nous avons rapporté trois bactéries qui n’étaient pas connues en littérature pour la production d’hydrogène. Dans la troisième partie de notre Thèse, nous avons exploré la flore sinusale à la recherche de méthanogènes. Nous avons pu mettre en évidence par des méthodes de biologie moléculaire, d’hybridation fluorescente in situ et de culture microbienne, des méthanogènes chez neuf patients différents présentant des infections sinusales. Nous nous sommes intéressées à rechercher les méthanogènes dans la cavité orale chez un autre type de population plus précisément dans un centre d’odonto-stomatologie au Mali. Au total, nous avons obtenu huit échantillons positifs pour les méthanogènes. Dans la dernière partie de notre Thèse, nous avons étudié le répertoire des archaea et bactéries associées à quinze espèces de plantes différentes utilisées comme brosses à dents naturelles à Bamako, au Mali.
In the first part of our thesis, we reviewed the literature on the methanogens found in the different microbiota of humans. We also reviewed the various methods used for the detection of these microorganisms in the clinical microbiology laboratory. In a second part of our Thesis work, we tested the hydrogen production by facultative anaerobic bacteria of clinical interest. , especially bacteria previously found associated with methanogens in clinical samples. We have observed that not all facultative anaerobic bacteria have the capacity to produce hydrogen gas and we have reported three bacteria that were not known in the literature for the production of hydrogen. In the third part of our Thesis, we explored the sinus flora in search of methanogens. Molecular biology, fluorescent in situ hybridization and microbial culture demonstrated methanogens in nine different patients with sinus infections. We were interested in looking for methanogens in the oral cavity in another type of population more specifically in a center of odonto-stomatology in Mali. In total, we obtained eight positive samples for methanogens. In the last part of our Thesis, we studied the archaea and bacteria repertoire associated with fifteen different plant species used as natural toothbrushes in Bamako, Mali
Advisors/Committee Members: Drancourt, Michel (thesis director).
Subjects/Keywords: Méthanogènes; Bactéries; Sinusite; Cavité orale; Brosse à dents végétales; Mali; Afrique; Methanogens; Bacteria; Sinusitis; Oral cavity; Natural toothbrush; Mali; Africa
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APA (6th Edition):
Sogodogo, E. (2019). Détection des méthanogènes dans la flore oro-sinusale : Detection of methanogens in the oro-sinus flora. (Doctoral Dissertation). Aix Marseille Université. Retrieved from http://www.theses.fr/2019AIXM0238
Chicago Manual of Style (16th Edition):
Sogodogo, Elisabeth. “Détection des méthanogènes dans la flore oro-sinusale : Detection of methanogens in the oro-sinus flora.” 2019. Doctoral Dissertation, Aix Marseille Université. Accessed April 17, 2021.
http://www.theses.fr/2019AIXM0238.
MLA Handbook (7th Edition):
Sogodogo, Elisabeth. “Détection des méthanogènes dans la flore oro-sinusale : Detection of methanogens in the oro-sinus flora.” 2019. Web. 17 Apr 2021.
Vancouver:
Sogodogo E. Détection des méthanogènes dans la flore oro-sinusale : Detection of methanogens in the oro-sinus flora. [Internet] [Doctoral dissertation]. Aix Marseille Université 2019. [cited 2021 Apr 17].
Available from: http://www.theses.fr/2019AIXM0238.
Council of Science Editors:
Sogodogo E. Détection des méthanogènes dans la flore oro-sinusale : Detection of methanogens in the oro-sinus flora. [Doctoral Dissertation]. Aix Marseille Université 2019. Available from: http://www.theses.fr/2019AIXM0238
19.
Sweidan, Alaa.
Antibiofilm activity of lichen secondary metabolites : Activité anti-biofilm des métabolites secondaires de lichen.
Degree: Docteur es, Biologie et Sciences de la Santé, 2017, Rennes 1
URL: http://www.theses.fr/2017REN1B017
► Les bactéries buccales n'infectent pas seulement la bouche mais y resident. Elles peuvent également passer dans la voie sanguine et atteindre des organes secondaires. S’il…
(more)
▼ Les bactéries buccales n'infectent pas seulement la bouche mais y resident. Elles peuvent également passer dans la voie sanguine et atteindre des organes secondaires. S’il n'est pas traité, le biofilm dentaire peut provoquer une inflammation destructrice dans la cavité buccale, entrainant de graves complications médicales. Dans ce biofilm, Streptococcus gordonii, colonisateur
oral primaire, constitue la plate-forme sur laquelle des colonisateurs pathogènes tardifs comme Porphyromonas gingivalis, l'agent causal des maladies parodontales, se lieront. L'objectif de la première partie de la thèse était de déterminer l'activité antibactérienne de onze composés de lichens appartenant à différentes familles chimiques, pour découvrir de nouveaux antibiotiques pouvant combattre ces bactéries buccales. Nous avons montré que trois composés avaient des activités antibactériennes prometteuses. L'acide psoromique enregistrait les CMIs le plus faibles. De nouveaux analogues de butyrolactone ont ensuite été conçus et synthétisés sur la base des composés antibactériens licheniques connus, les acides lichesteriniques, en substituant différents groupes fonctionnels sur le cycle butyrolactone pour améliorer son activité sur S. gordonii et P. gingivalis. Parmi les dérivés, B-12 et B-13 avaient la plus faible CMI où ils se sont révélés être des bactéricides plus forts, 2 à 3 fois plus, que l'antibiotique, doxycycline. B-12 et B-13 étaient également les plus efficaces vis-à-vis de P. gingivalis. La cytotoxicité de ces 2 composés a ensuite été vérifiée contre les cellulaires épithéliales gingivales humaines et les macrophages. Ils ne présentaient pas de toxicité contre les cellules testées. Une étude préliminaire de relation structure-activité a révélé le double rôle important apporté par deux substituants, chaîne alkyle en C5 et groupe carboxyle en C4 positions, dans leur mécanisme d'action. Ceci a été suivi par l'étude de l’activité antibiofilmique de B-12 et B-13 contre les deux souches orales en utilisant un test de cristal violet et microscopie confocale. Les deux dérivés ont montré, à une concentration plus faible, une inhibition maximale de la formation du biofilm, LCMI, de 9.38 μg/mL contre S. gordonii et 1.17 μg/mL contre P. gingivalis. Cependant, lorsque des concentrations sous-inhibitrices de B-12 et B-13 ont été utilisées, nous avons démontré que les deux souches étudiées pouvaient former des biofilms in vitro, accompagné d’une diminution de l'expression des gènes impliqués dans l'adhésion et la formation de biofilm. Pour mieux comprendre les mécanismes d'action des butyrolactones, nous avons étudié la localisation bactérienne du composé B-13 en synthétisant un B-13 marqué au NBD (4-nitro-benzo [1,2,5] oxadiazole) fluorescent conservant son activité antibactérienne. Par microscopie confocale et HPLC, nous avons montré que ce composé se lie à la surface cellulaire de S. gordonii. Ensuite, B-13 induit une rupture de la paroi cellulaire conduisant à la libération des constituants bactériens et par conséquent, à la mort de S.…
Advisors/Committee Members: Bousarghin, Latifa (thesis director), Van de Weghe, Pierre (thesis director), Tomasi, Sophie (thesis director), Chokr, Ali (thesis director).
Subjects/Keywords: Bactéries orales; Métabolites secondaires de lichen; Antimicrobien; Anti-Biofilm; Paroi cellulaire; Oral bacteria; Lichen secondary metabolites; Antimicrobial; Antibiofilm; Cell wall
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APA (6th Edition):
Sweidan, A. (2017). Antibiofilm activity of lichen secondary metabolites : Activité anti-biofilm des métabolites secondaires de lichen. (Doctoral Dissertation). Rennes 1. Retrieved from http://www.theses.fr/2017REN1B017
Chicago Manual of Style (16th Edition):
Sweidan, Alaa. “Antibiofilm activity of lichen secondary metabolites : Activité anti-biofilm des métabolites secondaires de lichen.” 2017. Doctoral Dissertation, Rennes 1. Accessed April 17, 2021.
http://www.theses.fr/2017REN1B017.
MLA Handbook (7th Edition):
Sweidan, Alaa. “Antibiofilm activity of lichen secondary metabolites : Activité anti-biofilm des métabolites secondaires de lichen.” 2017. Web. 17 Apr 2021.
Vancouver:
Sweidan A. Antibiofilm activity of lichen secondary metabolites : Activité anti-biofilm des métabolites secondaires de lichen. [Internet] [Doctoral dissertation]. Rennes 1; 2017. [cited 2021 Apr 17].
Available from: http://www.theses.fr/2017REN1B017.
Council of Science Editors:
Sweidan A. Antibiofilm activity of lichen secondary metabolites : Activité anti-biofilm des métabolites secondaires de lichen. [Doctoral Dissertation]. Rennes 1; 2017. Available from: http://www.theses.fr/2017REN1B017
University of Melbourne
20.
Fong, Shao Bing.
Characterisation of innate T cells in response to oral bacterial infection.
Degree: 2015, University of Melbourne
URL: http://hdl.handle.net/11343/91554
► Chronic periodontitis is an inflammatory disease of the supporting tissues of teeth that is characterised by bone resorption and if left untreated can result in…
(more)
▼ Chronic periodontitis is an inflammatory disease of the supporting tissues of teeth that is characterised by bone resorption and if left untreated can result in eventual tooth loss. The subgingival plaque bacteria Porphyromonas gingivalis, Treponema denticola and Tannerella forsythia have been closely associated with chronic periodontitis. T cell immunity during periodontal infections has been well-documented, mainly involving the adaptive immunity which includes Th1 and Th2 responses. A small number of clinical studies have reported the presence of IL-17 and Th17-related cytokines in the gingival crevicular fluid and diseased tissues of periodontitis patients. However, the roles of innate T cells and Th17-related responses in disease progression is unclear.
This study shows that NKT cells play a role in pro-inflammatory cytokine production, contributing to P. gingivalis-induced bone loss in the mouse periodontitis model. Inflammation and bone resorption was reduced in the absence of NKT cells or CD1d, the corresponding activating receptor for the TCR of NKT cells. As NKT cell responses were likely to be associated with glycolipid antigens, a major glycolipid of P. gingivalis was isolated, Pg-GL1, which was found to induce NKT cell and B cell activation. Results from knock-out mice studies suggested that NKT activation by Pg-GL1 occurs through a mixed requirement for TCR/CD1d-engagement and additional cytokines. Furthermore, Pg-GL1 was shown to induce the secretion of IL-1β, IL-6, IL-12(p40), IL-17, and G-CSF, cytokines that have been associated with inducing Th17-responses and, as contributing factors during periodontal inflammation and bone resorption.
Similar to P. gingivalis, three major glycolipids (termed Tf-GL1, Tf-GL2, Tf-GL3) of T. forsythia were found to highly activate NKT cells and B cells. Alkaline treatment of T. forsythia glycolipids revealed that Tf-GL2 was alkali-resistant, while Tf-GL1 and Tf-GL3 were susceptible and their deacylated forms showed the presence of complex carbohydrates. Mass spectrometry analysis revealed a wide structural variation in the carbohydrate head-group and acyl chain lengths. Base-labile Tf-GL1 and Tf-GL3 were identified to be diacylgylcerolipids while base-resistant Tf-GL2 was proposed to be a glycosphingolipid. The structure of the third glycolipid, Tf-GL3, was predicted to be highly complex and thus may have contributed to its lower antigenicity compared with Tf-GL1 and Tf-GL2. When the stimulatory ability of T. forsythia glycolipids was further investigated, only Tf-GL2 was able to induce IL-17 production when cultured with purified NKT cells and BMDCs. As only Tf-GL2 was able to induce cytokine production, it was concluded that structural characteristics play an important role in antigen potency.
When mice were orally infected with P. gingivalis, the CD27- γδ T cell sub-population in the maxillary epithelium was found to be activated after 14 days post-infection. Using pHrodo™ technology, it was shown that γδ T cells were able to phagocytose…
Subjects/Keywords: immunology; microbiology; innate immunity; NKT cells; gamma-delta T cells; periodontitis; glycolipids; porphyromonas gingivalis; Tannerella forsythia; oral bacteria; cytokines
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APA (6th Edition):
Fong, S. B. (2015). Characterisation of innate T cells in response to oral bacterial infection. (Doctoral Dissertation). University of Melbourne. Retrieved from http://hdl.handle.net/11343/91554
Chicago Manual of Style (16th Edition):
Fong, Shao Bing. “Characterisation of innate T cells in response to oral bacterial infection.” 2015. Doctoral Dissertation, University of Melbourne. Accessed April 17, 2021.
http://hdl.handle.net/11343/91554.
MLA Handbook (7th Edition):
Fong, Shao Bing. “Characterisation of innate T cells in response to oral bacterial infection.” 2015. Web. 17 Apr 2021.
Vancouver:
Fong SB. Characterisation of innate T cells in response to oral bacterial infection. [Internet] [Doctoral dissertation]. University of Melbourne; 2015. [cited 2021 Apr 17].
Available from: http://hdl.handle.net/11343/91554.
Council of Science Editors:
Fong SB. Characterisation of innate T cells in response to oral bacterial infection. [Doctoral Dissertation]. University of Melbourne; 2015. Available from: http://hdl.handle.net/11343/91554
21.
Romi, Fadi.
Effect of Stevia on oral biofilms - a pilot study in vitro.
Degree: 2016, , Faculty of Odontology (OD)
URL: http://urn.kb.se/resolve?urn=urn:nbn:se:mau:diva-19811
► Syfte Syftet med denna laborativa pilotstudie in vitro var att undersöka hur den mikrobiella sammansättningen och pH i den orala biofilmen påverkades vid upprepad…
(more)
▼ Syfte
Syftet med denna laborativa pilotstudie in vitro var att undersöka hur den mikrobiella sammansättningen och pH i den orala biofilmen påverkades vid upprepad exponering för Stevia (steviosid och rebaudiosid A), sackaros eller en kombination av reb A och sackaros. Ett ytterligare syfte var att studera om isolat av orala bakterier kunde metabolisera reb A i buljongkultur.
Material och metod
Biofilmer från tre personer blev exponerade för 0,025 % steviosid, 0,025 % reb A eller 10 % Todd Hewitt (TH) under 15 minuter tre gånger dagligen i fyra dagar. Artbestämning gjordes i början och slutet av försöken. Biofilmerna exponerades för 0,025 % reb A och 10 % sackaros eller en kombination av dessa varefter pH-värdet mättes. Vidare studerades 23 bakteriestammar avseende förmåga att metabolisera 0,025% respektive 0,5% reb A.
Resultat
Streptokocker dominerade i samtliga biofilmer vid försökets slut oavsett om de exponerats för steviosid, reb A eller enbart TH.
En pH-sänkning kunde ses hos biofilmerna som enbart blivit exponerade för sackaros eller för en kombination av sackaros och reb A. Däremot kunde endast en marginell pH-sänkning observeras efter exponering för enbart reb A.
Ingen av de enskilda bakteriestammar som studerades kunde metabolisera reb A.
Slutsats
Ingen större skillnad i biofilmens bakteriesammansättning kunde ses efter upprepad exponering för steviosid eller reb A i förhållande till TH.
Upprepade exponeringar av reb A i kombination med sackaros gav en liknande pH-sänkning som enbart sackaros, dock sågs ingen pH-sänkning efter exponering för enbart reb A.
Enskilda bakteriestammar kunde inte metabolisera reb A.
Aims
In this in vitro laboratory pilot study, the effect on the microbial composition and pH of the oral biofilms after repeated exposure to Stevia (stevioside and rebaudioside A), saccarose or a combination, was investigated. Another aim was to study whether isolate of oral bacteria were able to metabolise reb A in broth bacteria culture.
Material and Method
Biofilms from three adult individuals were repeatedly exposed to 0,025% stevioside, 0,025% reb A or 10% Todd Hewitt (TH). Samples were taken at the beginning and at the end of the experiment for cultivation and bacterial identification.
Biofilms were exposed to 0,025% reb A and 10% saccharose, either separately or combined, followed by a pH-measurement. Furthermore, 23 bacterial species were studied in regards to ability to metabolize reb A.
Results
All biofilms were dominated by Streptococcus regardless of whether they were exposed to stevioside, reb A, or TH.
A pH-decrease was seen in biofilms exposed to saccharose or a combination of saccharose and reb A. However, only minor changes were noted after exposure to reb A.
None of the species studied were able to metabolize reb A.
Conclusion
No major differences in the bacterial composition of the biofilms were seen after repeated exposure to stevioside or reb A compared to TH.
No pH-decrease after repeated exposure to reb A was noted. Exposure to reb A combined with…
Subjects/Keywords: Plaque pH; Stevia; Stevioside; Rebaudiosid A; Oral biofilm; Bacteria; Caries; Medical and Health Sciences; Medicin och hälsovetenskap
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APA (6th Edition):
Romi, F. (2016). Effect of Stevia on oral biofilms - a pilot study in vitro. (Thesis). , Faculty of Odontology (OD). Retrieved from http://urn.kb.se/resolve?urn=urn:nbn:se:mau:diva-19811
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Romi, Fadi. “Effect of Stevia on oral biofilms - a pilot study in vitro.” 2016. Thesis, , Faculty of Odontology (OD). Accessed April 17, 2021.
http://urn.kb.se/resolve?urn=urn:nbn:se:mau:diva-19811.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Romi, Fadi. “Effect of Stevia on oral biofilms - a pilot study in vitro.” 2016. Web. 17 Apr 2021.
Vancouver:
Romi F. Effect of Stevia on oral biofilms - a pilot study in vitro. [Internet] [Thesis]. , Faculty of Odontology (OD); 2016. [cited 2021 Apr 17].
Available from: http://urn.kb.se/resolve?urn=urn:nbn:se:mau:diva-19811.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Romi F. Effect of Stevia on oral biofilms - a pilot study in vitro. [Thesis]. , Faculty of Odontology (OD); 2016. Available from: http://urn.kb.se/resolve?urn=urn:nbn:se:mau:diva-19811
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Universidade do Rio Grande do Norte
22.
Dantas, Emanuelle Dayana Vieira.
Efeito in vitro da terapia fotodinâmica sobre bactérias orais em suspensão e na formação de biofilme : ensaio com azul de metileno e toluidina ativados por luz halógena
.
Degree: 2011, Universidade do Rio Grande do Norte
URL: http://repositorio.ufrn.br/handle/123456789/17079
► Photodynamic therapy (PDT) has been proposed as an alternative method for the treatment of biofilm-dependent oral diseases like dental caries. This therapy consists of simultaneous…
(more)
▼ Photodynamic therapy (PDT) has been proposed as an alternative method for the treatment of biofilm-dependent
oral diseases like dental caries. This therapy consists of simultaneous action of a visible light (L) and a photosensitizer (FS) in the presence of oxygen, which leads to production of different reactive oxygen species that can interact with the bacterial cell components, and promote cell death. This study aims to evaluate the antimicrobial action of PDT on
oral bacteria in suspension, as well as the formation of mono and multi-species biofilms, in vitro, from a standard strain of Streptococcus mutans (ATCC 25175) and saliva samples, respectively. The dye methylene blue (MB) and toluidine blue (TB) were used at a concentration of 100 mg/ L and activated by halogen light (600 to 750 nm) from a modified hand held photopolymerizer (Ultralux ®, Dabi Atlante, Ribeirão Preto , São Paulo, Brazil.). Planktonic cultures were prepared and submitted to different experimental conditions: 1. PDT using TB 2. PDT using MB, 3. L+ FS- , 4. TB + L - ; 5. MB+ L-; 6. L- FS- (negative control) and 7. administration of 0.12% chlorhexidine digluconate (positive control) (Periogard ®, Colgate-Palmolive Company, New York, USA). The immediate and mediated action of PDT on bacterial suspensions, as well as its effect on biofilm formation were observed from the number of colony-forming units per milliliter (CFU/mL) and measures optical density (OD). The data were statistically analyzed using the Kruskal-Wallis and Mann-Whitney test for the significance level of 5%. According to the results, the PDT showed no antibacterial action on suspensions of S. mutans, regardless of the dye used. PDT with MB activated by halogen light was able to reduce 86.6% CFU/mL multi-species planktonic cultures, however, this reduction was not significant (p > 0.05). PDT showed antibacterial effect, mediate on multi-species planktonic cultures with TB (p < 0.001) and MB (p < 0.001), activated by halogen light. PDT was able to prevent the formation of multispecies biofilm, through the activation of TB by halogen light (p = 0.01). We conclude that activation of the dye toluidine blue and methylene blue, by halogen light (PDT) showed antimicrobial activity, compared to multi-species planktonic cultures prepared from saliva samples
Advisors/Committee Members: Lima, Kenio Costa de (advisor), CPF:50337157472 (advisor), http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4723244A9 (advisor).
Subjects/Keywords: Terapia fotodinâmica. Luz halógena. Azul de metileno. Azul de toluidina. Bactérias orais. S. mutans. Cárie dentária.;
Photodynamic therapy. Halogen light. Methylene blue. Toluidine blue. Oral bacteria. S. mutans. Dental caries.
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APA (6th Edition):
Dantas, E. D. V. (2011). Efeito in vitro da terapia fotodinâmica sobre bactérias orais em suspensão e na formação de biofilme : ensaio com azul de metileno e toluidina ativados por luz halógena
. (Thesis). Universidade do Rio Grande do Norte. Retrieved from http://repositorio.ufrn.br/handle/123456789/17079
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Dantas, Emanuelle Dayana Vieira. “Efeito in vitro da terapia fotodinâmica sobre bactérias orais em suspensão e na formação de biofilme : ensaio com azul de metileno e toluidina ativados por luz halógena
.” 2011. Thesis, Universidade do Rio Grande do Norte. Accessed April 17, 2021.
http://repositorio.ufrn.br/handle/123456789/17079.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Dantas, Emanuelle Dayana Vieira. “Efeito in vitro da terapia fotodinâmica sobre bactérias orais em suspensão e na formação de biofilme : ensaio com azul de metileno e toluidina ativados por luz halógena
.” 2011. Web. 17 Apr 2021.
Vancouver:
Dantas EDV. Efeito in vitro da terapia fotodinâmica sobre bactérias orais em suspensão e na formação de biofilme : ensaio com azul de metileno e toluidina ativados por luz halógena
. [Internet] [Thesis]. Universidade do Rio Grande do Norte; 2011. [cited 2021 Apr 17].
Available from: http://repositorio.ufrn.br/handle/123456789/17079.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Dantas EDV. Efeito in vitro da terapia fotodinâmica sobre bactérias orais em suspensão e na formação de biofilme : ensaio com azul de metileno e toluidina ativados por luz halógena
. [Thesis]. Universidade do Rio Grande do Norte; 2011. Available from: http://repositorio.ufrn.br/handle/123456789/17079
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
23.
Orona, Gabriella Marie.
Bacterial Lipopolysaccharides Induce Suppression of Natural Killer Cell Function.
Degree: Oral Biology, 2012, UCLA
URL: http://www.escholarship.org/uc/item/6rn8w3b0
► Natural Killer (NK) cells comprise a unique subset of lymphocytes with innate ability to detect and kill abnormal cells. Autologous NK cells and mature dendritic…
(more)
▼ Natural Killer (NK) cells comprise a unique subset of lymphocytes with innate ability to detect and kill abnormal cells. Autologous NK cells and mature dendritic cells (DC) mutually activate each other and this interaction is believed to be important for NK cytotoxic activity against cancer cells. However, it has been shown that NK cell mediated lysis is greatly suppressed after interaction with stem cells [1-3]. It is believed that a phenomenon called split angery occurs in activated NK cells, causing the cells to lose their cytotoxic functions, but gain the ability to secrete cytokines. Understanding the interactions that take place between NK cells and the tumor microenvironment, and how these immune cells are able to specifically target virally infected and cancerous cells, but not healthy cells, is crucial for future developments of more effective preventative and therapeutic approaches in the battle of infectious diseases and cancer. In this study we hypothesized that oral and intestinal bacteria promote differentiation of the Oral Squamous Cancer Stem Cells (OSCSCs) and the Colorectal carcinoma cell line (HT29) through two primary mechanisms. The first, being through direct activation of OSCSCs and HT29s, and the second being through the induction of split anergy in interacting NK cells.Patient derived oral tumors and colorectal carcinomas were used in a standard 51Chromium release assay to determine their sensitivity or resistance against NK cell mediated lysis. The secretion of key cytokines by NK cells, such as Interferon- Gamma (IFN-γ), were determined using Enzyme-Linked Immunosorbent Assays (ELISAs). Experimental findings of this project demonstrate that the engagement of CD16 receptors on NK cells or interaction with monocytes, Fusobacterium nucleatum, bacterial LPS, or AKF-1 (a combination of eight bacterial strains) lead to the support of differentiation of stem cells via increased cytokine secretion by NK cells.
Subjects/Keywords: Immunology; Bacteria; Cancer; Fuso nucleatum; Natural Killer Cells; Oral Immunology
…Cytokines …..7
Bacteria and the immune system .8… …differentiation of the Oral Squamous Cancer
Stem Cells (OSCSCs).
Introduction… …vii
LIST OF FIGURES
1. Treatment of Oral Squamous Cancer Stem Cells (OSCSCs)… …mediated cytotoxicity.
2. Treatment of Oral Squamous Cancer Stem Cells (OSCSCs) with… …hypothetical model of NK cell anergy.
14. A schematic representation of hypothetical model of oral…
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APA (6th Edition):
Orona, G. M. (2012). Bacterial Lipopolysaccharides Induce Suppression of Natural Killer Cell Function. (Thesis). UCLA. Retrieved from http://www.escholarship.org/uc/item/6rn8w3b0
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Orona, Gabriella Marie. “Bacterial Lipopolysaccharides Induce Suppression of Natural Killer Cell Function.” 2012. Thesis, UCLA. Accessed April 17, 2021.
http://www.escholarship.org/uc/item/6rn8w3b0.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Orona, Gabriella Marie. “Bacterial Lipopolysaccharides Induce Suppression of Natural Killer Cell Function.” 2012. Web. 17 Apr 2021.
Vancouver:
Orona GM. Bacterial Lipopolysaccharides Induce Suppression of Natural Killer Cell Function. [Internet] [Thesis]. UCLA; 2012. [cited 2021 Apr 17].
Available from: http://www.escholarship.org/uc/item/6rn8w3b0.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Orona GM. Bacterial Lipopolysaccharides Induce Suppression of Natural Killer Cell Function. [Thesis]. UCLA; 2012. Available from: http://www.escholarship.org/uc/item/6rn8w3b0
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
University of Manitoba
24.
Nistor, Andreea.
Investigations into the role of mPIP, the mouse homologue of hPIP/GCDFP-15, in innate host defense.
Degree: Pathology, 2008, University of Manitoba
URL: http://hdl.handle.net/1993/3031
► mPIP is a mouse homologue of human PIP/GCDFP-15 which is an established marker of both malignant and benign pathological conditions of the mammary gland. mPIP…
(more)
▼ mPIP is a mouse homologue of human PIP/GCDFP-15 which is an established marker of both malignant and benign pathological conditions of the mammary gland. mPIP gene expression has been identified in both lacrimal and salivary glands of healthy mice and the mPIP protein has been detected in saliva. The mPIP protein has been found to bind
oral bacteria, showing the highest affinity for streptococci, suggesting a potential function of mPIP in the non-immune host defense in the mouse
oral cavity. Since the exact functions of mPIP are still unknown, we examined the roles of mPIP through both in vitro and in vivo studies, specifically to address the possible role of this protein in non-immune host response through modulating the
oral flora.
The in vitro studies were primarily focused on elucidation of the consequences of interaction between mPIP and
oral bacteria, in particular to examine whether mPIP plays a role in bacterial aggregation. The in vivo studies addressed the roles of mPIP through the analysis of an mPIP knockout mouse model generated in our laboratory. Following confirmation of the null mutation, the delineating the phenotype of this model was pursued through morphopathological analysis as well as examination of the impact of the lack of mPIP on the mouse
oral flora.
The null mutation in the mPIP knockout mice was confirmed by both the gene and protein analysis. Histological analysis revealed lymphocytic proliferation in both the submaxillary and prostate glands of the mPIP knockout mice. In addition, both quantitative and composition differences in the
oral flora of mPIP knockout mice were identified when compared with wild-type controls. Specifically, a higher proportion of the
oral bacteria of mPIP knockout mice were found to belong to genus Streptococcus and certain genera were found to be absent from the
oral cavity of these mice. The effect of knockout mouse saliva, which lacks mPIP, on the aggregation of
oral bacteria was compared to wild-type mouse saliva. Our data suggests that mPIP contributes to saliva-induced bacterial aggregation.
While
oral flora has multiple functions, including protection against infection, mPIP might play a role in the non-innate host defense through modulating the resident
oral flora in the mouse. The identification of lymphocytic proliferation in submaxillary and prostate glands of mPIP knockout mice suggests that mPIP might also interfere with lymphocyte activity, playing a possible immunomodulatory role.
Advisors/Committee Members: Myal, Yvonne (Pathology) (supervisor), Wigle, Jeffrey (Biochemistry and Medical Genetics).
Subjects/Keywords: innate host defense; knockout mouse; oral flora; protein-bacteria interaction
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APA (6th Edition):
Nistor, A. (2008). Investigations into the role of mPIP, the mouse homologue of hPIP/GCDFP-15, in innate host defense. (Masters Thesis). University of Manitoba. Retrieved from http://hdl.handle.net/1993/3031
Chicago Manual of Style (16th Edition):
Nistor, Andreea. “Investigations into the role of mPIP, the mouse homologue of hPIP/GCDFP-15, in innate host defense.” 2008. Masters Thesis, University of Manitoba. Accessed April 17, 2021.
http://hdl.handle.net/1993/3031.
MLA Handbook (7th Edition):
Nistor, Andreea. “Investigations into the role of mPIP, the mouse homologue of hPIP/GCDFP-15, in innate host defense.” 2008. Web. 17 Apr 2021.
Vancouver:
Nistor A. Investigations into the role of mPIP, the mouse homologue of hPIP/GCDFP-15, in innate host defense. [Internet] [Masters thesis]. University of Manitoba; 2008. [cited 2021 Apr 17].
Available from: http://hdl.handle.net/1993/3031.
Council of Science Editors:
Nistor A. Investigations into the role of mPIP, the mouse homologue of hPIP/GCDFP-15, in innate host defense. [Masters Thesis]. University of Manitoba; 2008. Available from: http://hdl.handle.net/1993/3031
University of Kentucky
25.
Denney, Hilary.
Listeria Monocytogenes can Utilize both M Cell Transcytosis and InlA-Mediated Uptake to Cross the Epithelial Barrier of the Intestine during an Oral Infection Model of Listeriosis.
Degree: 2014, University of Kentucky
URL: https://uknowledge.uky.edu/medsci_etds/3
► The invasive pathways, InlA- and InB-mediated uptake and M cell transcytosis, that Listeria monocytogenes uses to invade the intestine have mainly been studied using infection…
(more)
▼ The invasive pathways, InlA- and InB-mediated uptake and M cell transcytosis, that Listeria monocytogenes uses to invade the intestine have mainly been studied using infection models that do not truly replicate what occurs during a natural infection. Recently, our lab has developed an oral infection model that is more physiolocally relevant to what occurs during food borne listeriosis. We have sought to evaluate the relative roles of the previously defined invasive pathways, in our oral model of infection. We have done this by utilizing an InlAmCG Lm strain that is able to bind murine E-cadherin, knockout Lm strains, ΔinlA Lm, and ΔinlAΔinlB Lm. We also took advantage of a knockout mice strain CD137-/-that has M cells that are deficient in M cell transcytosis. We were able to show that these invasive pathways are relevant in our oral infection model, that M cell transcytosis is a compensatory pathway for InlA-mediated uptake, and that there might be another mechanism that L. monocytogenes uses to invade the intestines. To confirm this, it is necessary though that the M cell transcytosis deficiency be confirmed in the CD137-/- mice.
Subjects/Keywords: Listeria monocytogenes; oral infection model; InlA – mediated uptake; M cell transcytosis; invasion; Bacteria; Bacterial Infections and Mycoses; Bacteriology; Medical Microbiology; Other Microbiology; Pathogenic Microbiology
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APA (6th Edition):
Denney, H. (2014). Listeria Monocytogenes can Utilize both M Cell Transcytosis and InlA-Mediated Uptake to Cross the Epithelial Barrier of the Intestine during an Oral Infection Model of Listeriosis. (Masters Thesis). University of Kentucky. Retrieved from https://uknowledge.uky.edu/medsci_etds/3
Chicago Manual of Style (16th Edition):
Denney, Hilary. “Listeria Monocytogenes can Utilize both M Cell Transcytosis and InlA-Mediated Uptake to Cross the Epithelial Barrier of the Intestine during an Oral Infection Model of Listeriosis.” 2014. Masters Thesis, University of Kentucky. Accessed April 17, 2021.
https://uknowledge.uky.edu/medsci_etds/3.
MLA Handbook (7th Edition):
Denney, Hilary. “Listeria Monocytogenes can Utilize both M Cell Transcytosis and InlA-Mediated Uptake to Cross the Epithelial Barrier of the Intestine during an Oral Infection Model of Listeriosis.” 2014. Web. 17 Apr 2021.
Vancouver:
Denney H. Listeria Monocytogenes can Utilize both M Cell Transcytosis and InlA-Mediated Uptake to Cross the Epithelial Barrier of the Intestine during an Oral Infection Model of Listeriosis. [Internet] [Masters thesis]. University of Kentucky; 2014. [cited 2021 Apr 17].
Available from: https://uknowledge.uky.edu/medsci_etds/3.
Council of Science Editors:
Denney H. Listeria Monocytogenes can Utilize both M Cell Transcytosis and InlA-Mediated Uptake to Cross the Epithelial Barrier of the Intestine during an Oral Infection Model of Listeriosis. [Masters Thesis]. University of Kentucky; 2014. Available from: https://uknowledge.uky.edu/medsci_etds/3
26.
CAVALHEIRO, FLAVIA M.
Avaliação da redução microbiana em condutos radiculares contaminados comparando três técnicas de irradiacao com laser de baixa potência associado a fotossensibilizador.
Degree: 2007, Brazil
URL: http://repositorio.ipen.br:8080/xmlui/handle/123456789/11639
► Made available in DSpace on 2014-10-09T12:54:12Z (GMT). No. of bitstreams: 0
Made available in DSpace on 2014-10-09T14:09:28Z (GMT). No. of bitstreams: 1 12696.pdf: 380416 bytes,…
(more)
▼ Made available in DSpace on 2014-10-09T12:54:12Z (GMT). No. of bitstreams: 0
Made available in DSpace on 2014-10-09T14:09:28Z (GMT). No. of bitstreams: 1 12696.pdf: 380416 bytes, checksum: b41e65f3de160c24632b1af1ee4ac0ee (MD5)
Dissertacao (Mestrado Profissionalizante em Lasers em Odontologia)
IPEN/D-MPLO
Instituto de Pesquisas Energeticas e Nucleares - IPEN/CNEN-SP; Faculdade de Odontologia, Universidade de São Paulo , São Paulo
Advisors/Committee Members: Sheila Gouw-Soares, Martha Simoes Ribeiro.
Subjects/Keywords: endodontic therapy; oral cavity; contamination; bacteria; photodynamic therapy; laser; photosensitivity
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APA (6th Edition):
CAVALHEIRO, F. M. (2007). Avaliação da redução microbiana em condutos radiculares contaminados comparando três técnicas de irradiacao com laser de baixa potência associado a fotossensibilizador. (Masters Thesis). Brazil. Retrieved from http://repositorio.ipen.br:8080/xmlui/handle/123456789/11639
Chicago Manual of Style (16th Edition):
CAVALHEIRO, FLAVIA M. “Avaliação da redução microbiana em condutos radiculares contaminados comparando três técnicas de irradiacao com laser de baixa potência associado a fotossensibilizador.” 2007. Masters Thesis, Brazil. Accessed April 17, 2021.
http://repositorio.ipen.br:8080/xmlui/handle/123456789/11639.
MLA Handbook (7th Edition):
CAVALHEIRO, FLAVIA M. “Avaliação da redução microbiana em condutos radiculares contaminados comparando três técnicas de irradiacao com laser de baixa potência associado a fotossensibilizador.” 2007. Web. 17 Apr 2021.
Vancouver:
CAVALHEIRO FM. Avaliação da redução microbiana em condutos radiculares contaminados comparando três técnicas de irradiacao com laser de baixa potência associado a fotossensibilizador. [Internet] [Masters thesis]. Brazil; 2007. [cited 2021 Apr 17].
Available from: http://repositorio.ipen.br:8080/xmlui/handle/123456789/11639.
Council of Science Editors:
CAVALHEIRO FM. Avaliação da redução microbiana em condutos radiculares contaminados comparando três técnicas de irradiacao com laser de baixa potência associado a fotossensibilizador. [Masters Thesis]. Brazil; 2007. Available from: http://repositorio.ipen.br:8080/xmlui/handle/123456789/11639
University of Louisville
27.
Koneru, Lahari.
Type IX secretion system : characterization of an effector protein and an insight into the role of c-terminal domain dimeration in outer membrane translocation.
Degree: PhD, 2017, University of Louisville
URL: 10.18297/etd/2854
;
https://ir.library.louisville.edu/etd/2854
► Porphyromonas gingivalis and Tannerella forsythia are two of the primary pathogens that are associated in the etiology and progression of chronic periodontitis. In T.…
(more)
▼ Porphyromonas gingivalis and
Tannerella forsythia are two of the primary pathogens that are associated in the etiology and progression of chronic periodontitis. In
T. forsythia, KLIKK proteases are the recently identified group of proteolytic enzymes that are secreted through Type IX secretion system (T9SS). Among, these KLIKK proteases a synergistic relationship was observed between karilysin and mirolysin in invading the host complement system for the survival of the
bacteria. Since, karilysin has been already characterized, in this study we propose to study about mirolysin through structural, biochemical and biological characterization. The obtained results from the experiments has shown the propensity of the proenzyme (mirolysin) to autocatalytically process itself at Xaa-Arg peptide bonds. Also, the catalytic enzyme has shown the capablity to degrade an array of physiological substrates like fibrinogen, fibronectin, insulin and LL-37 antimicrobial peptide. In
P. gingivalis, gingipains are the major proteolytic enzymes that are also secreted through T9SS and possess a conserved CTD similar to KLIKK proteases. The conserved C-Terminal Domain (CTD) of the T9SS cargo proteins is found essential for the export of these proteins from the periplasm across the outer membrane. Although the mechanism of CTD as an export signal is not well understood so far, taking previous literature into consideration a phenomenon of CTD dimerization has been hypothesized as a possible mechanism in translocation of the cargo proteins through T9SS. Hence, based on the structure of an already crystallized rCTD of RgpB [95] and PorZ (PG1604), in which the CTD is not cleaved off during the translocation of PorZ across outer membrane, four mutations (A637R, G699R, A719R, and R721E) were incorporated into the structure of the RgpB CTD. These mutants were later characterized for their secretory phenotype by quantification of the gingipain activity and by analyzing the processing of the protein through western blots. Out of the four, G699R and A719R mutations have completely occluded the export of protein, while the proteins bearing A637R and R721E mutations were partially processed and secreted. The full-length ProRgpB (native) and its recombinant Ig-CTD tandem proteins, with and without insertion of the factor Xa cleavage site (inserted between Ig and CTD), were purified to investigate the effect of A719R and R721E mutations on CTD dimerization. Preliminary results with the glutaraldehyde crosslinking on recombinant Ig-CTD proteins with A719R and R721E mutations revealed the lack of dimer formation in contrast to the wild-type recombinant Ig-CTD protein without any mutations. In future, these studies will certainly help in understanding the mechanism of T9SS in Gram-negative
bacteria.
Advisors/Committee Members: Potempa, Jan, Scott, David A., Scott, David A., Lamont, Richard J., Uriarte, Silvia M..
Subjects/Keywords: type IX secretion system (T9SS); porphyromonas gingivalis; tannerella forsythia; proteases; mirolysin; c-terminal domain; Bacteria; Bacteriology; Biochemistry; Immunology of Infectious Disease; Molecular Biology; Oral Biology and Oral Pathology; Periodontics and Periodontology; Structural Biology
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APA (6th Edition):
Koneru, L. (2017). Type IX secretion system : characterization of an effector protein and an insight into the role of c-terminal domain dimeration in outer membrane translocation. (Doctoral Dissertation). University of Louisville. Retrieved from 10.18297/etd/2854 ; https://ir.library.louisville.edu/etd/2854
Chicago Manual of Style (16th Edition):
Koneru, Lahari. “Type IX secretion system : characterization of an effector protein and an insight into the role of c-terminal domain dimeration in outer membrane translocation.” 2017. Doctoral Dissertation, University of Louisville. Accessed April 17, 2021.
10.18297/etd/2854 ; https://ir.library.louisville.edu/etd/2854.
MLA Handbook (7th Edition):
Koneru, Lahari. “Type IX secretion system : characterization of an effector protein and an insight into the role of c-terminal domain dimeration in outer membrane translocation.” 2017. Web. 17 Apr 2021.
Vancouver:
Koneru L. Type IX secretion system : characterization of an effector protein and an insight into the role of c-terminal domain dimeration in outer membrane translocation. [Internet] [Doctoral dissertation]. University of Louisville; 2017. [cited 2021 Apr 17].
Available from: 10.18297/etd/2854 ; https://ir.library.louisville.edu/etd/2854.
Council of Science Editors:
Koneru L. Type IX secretion system : characterization of an effector protein and an insight into the role of c-terminal domain dimeration in outer membrane translocation. [Doctoral Dissertation]. University of Louisville; 2017. Available from: 10.18297/etd/2854 ; https://ir.library.louisville.edu/etd/2854
28.
Dorkhan, Marjan.
Effect of surface characteristics on cellular adherence and activity
.
Degree: Malmö University. Faculty of Odontology, 2014, Malmö University
URL: http://hdl.handle.net/2043/17030
► After insertion of the dental implant into the jaw, the neck of the implant protruding through the mucosa (implant abutment) will be exposed to the…
(more)
▼ After insertion of the dental implant into the jaw, the neck of the implant protruding through the mucosa (implant abutment) will be exposed to the complex environment of the mouth. This results in the formation of a conditioning protein coat (pellicle) derived from saliva and/or gingival crevicular fluid. Microorganisms in saliva are transported to the surfaces where they initiate biofilm (plaque) formation. Over time, early colonizers promote co-aggregation of later colonizers, leading to development of complex plaque, which can include hundreds of different bacterial species. Continuous undisturbed growth of plaque has been reported to trigger inflammatory responses in the periodontal tissues, which can compromise the integration of the implant abutment with the surrounding oral mucosa and eventually progress to breakdown of supporting bone tissue (peri-implant disease).
Key elements in the long-term success of dental implants are the formation of a stable connection between the sub-crestal anchoring part of the implant (fixture) and the host bone tissue (osseointegration) and integration of the abutment with the surrounding soft tissues. Consequently, much research has been focused on development of surfaces that may optimize osseointegration as well as support the formation of a healthy cuff of keratinized mucosa around the implant abutment, providing a barrier that prevents the passage of microorganisms into the underlying connective tissues. Reports from a large number of studies have shed light upon the positive effects of surface modifications on osseointegration. However, the effect of such modifications on development of oral biofilms and soft-tissue cells is not understood.
The overall aim of this thesis was to obtain a better understanding of the adaptive processes occurring at the implant-host tissue interface. Thus the effects of surface characteristics on formation of pellicles as well as adherence and activity of early colonizing bacteria were examined. Furthermore, we investigated adherence of epithe- lial cells and fibroblasts to nano-porous titanium surfaces in order to identify surface characteristics that may facilitate improved soft tissue attachment.
In paper I, the effects of surface roughness as well as the effect of a saliva- or serum- derived coating on adherence of different strains of Streptococcus oralis (an early colonizer of mouth that is also recovered from implant surfaces in vivo) to titanium was examined. Titanium plates with smooth (average height deviation (Sa) < 0.5 μm) or moderately rough (Sa 1-2 μm) surface topography were used together with a flow-cell model and confocal laser scanning microscopy (CLSM) with Live/Dead BacLight staining kit. Microbial adherence to moderately rough surfaces was greater overall than that to smooth surfaces, suggesting that implants with moderately rough surfaces, developed to improve osseointegration, have a greater propensity for retention of adhered bacteria. Furthermore, a saliva pellicle promoted binding of S. oralis although different…
Subjects/Keywords: Bacteria;
microbial biofilm;
dental implant;
streptococci;
oral bacteria;
salivary pellicle;
early colonizers;
titanium oxide;
microbial activity;
oral keratinocytes;
gingival fibroblasts;
cell attachment;
surface modification;
anodic oxidation;
anatase
…adherence and activity of oral bacteria. In particular, anataserich, nano-porous surfaces appear… …Plaque or the oral biofilm is a sticky complex of bacteria that accumulates on the hard tissues… …Interactions between oral bacteria and implant surfaces
After introduction of dental implants into… …and the
composition of the attached pellicles on adherence and
activity of oral bacteria… …tissue cell cultures ........................................................... 36
Human oral…
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APA (6th Edition):
Dorkhan, M. (2014). Effect of surface characteristics on cellular adherence and activity
. (Thesis). Malmö University. Retrieved from http://hdl.handle.net/2043/17030
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Dorkhan, Marjan. “Effect of surface characteristics on cellular adherence and activity
.” 2014. Thesis, Malmö University. Accessed April 17, 2021.
http://hdl.handle.net/2043/17030.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Dorkhan, Marjan. “Effect of surface characteristics on cellular adherence and activity
.” 2014. Web. 17 Apr 2021.
Vancouver:
Dorkhan M. Effect of surface characteristics on cellular adherence and activity
. [Internet] [Thesis]. Malmö University; 2014. [cited 2021 Apr 17].
Available from: http://hdl.handle.net/2043/17030.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Dorkhan M. Effect of surface characteristics on cellular adherence and activity
. [Thesis]. Malmö University; 2014. Available from: http://hdl.handle.net/2043/17030
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Universidade do Rio Grande do Norte
29.
Vieira, Liza Barreto.
Eficácia do bochecho de quitosana a 0,4% sobre o biofilme e bactérias orais
.
Degree: 2006, Universidade do Rio Grande do Norte
URL: http://repositorio.ufrn.br/handle/123456789/17033
► The purpose of this study was evaluate the effectiveness of the chitosan at 0.4 with high molecular weight and high deacetylation degree mouthrinse over the…
(more)
▼ The purpose of this study was evaluate the effectiveness of the chitosan at 0.4 with high molecular weight and high deacetylation degree mouthrinse over the total decrease of the streptococci, Streptococcus mutans, lactobaci/li and over the perceptible bacterial film and gingival bleeding indices. For that, a total of 68 healthy students between 11 and 13 years old, not allergic to crustacean and not users of antibiotics or antimicrobial agent for the last three months or during the treatment, was selected. From those, thirty two individuaIs used the mouthrinse test, and thirty six, the control one. The participants rinsed 10 mL of the solutions twice a day, one during the moming (which was supervised), and another one during the aftemoon (which was not supervised), for fifteen days. The saliva collect for the microbiological analysis, as well as the perceptible bacterial film and gingival bleeding indices check, were made before the use ofthe mouthrinses (base line), immediately after the last mouthrinse on the day (zero time) and fifteen days after (fifteen time). These data were collected at school and the saliva was carried inside the ice to the laboratory. The samples were diluted, and 0.1 mL ofthe 10 -1 dilution was seeded in Rogosa SL agar, for further analysis of the total of lactobaci/lus~ 0.1 mL of the 10-4 dilution in Mitis Salivarius with bacitracin, for S. mutans analysis; and 0.1 mL of the 10-6 dilution in Mitis Salivarius for the analysis ofthe total of streptococcus. The Rogosa SL agar plates were incubated in aerobic at 37°C for 72 hours and the MSB and the MS were incubated in anaerobic in
[email protected] jars at 37°C for 48 hours for further count ofColonies Former Units (CFUs). The assay was made in duplicate for each bacterial group analyzed. The number of CFUs transformed in LOGlO was analyzed according to the following tests: ANOV A, t of Paired and Not Paired Student, Friedman, Man-Whitney and square-qui test. On the base line, alI the variables analyzed were similar on both tested groups. On both groups, for the total of streptococcus there was no significant difference along the time and for S. mutans there was a statistic significant increase of the CFUs from the base line to the zero time. For the total of lactobaccilus there was no significant difference on the test group along the time, and on the control there was a significant increase ofthe CFUs ITom the base line to the zero time. For both groups, there was significant decrease ofthe perceptible bacterial film index along the time, and that can be explained by the mechanic effect of the mouthrinse over the bacterial film and by the participation of the students on the research which could have motivated him to a better toothbrushing (Hawthome effect). The gingival bleeding index also showed a decrease along the time, even though it was not significant. Therefore, the conclusion of this study was that the chitosan at 0.4 % mouthrinse was not effective on the CFUs reduction of the three bacterial groups analyzed, as well as on the reduction of…
Advisors/Committee Members: Lima, Kenio Costa de (advisor), CPF:50337157472 (advisor), http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4723244A9 (advisor).
Subjects/Keywords: Quitosana;
Bochecho;
Agente antimicrobiano;
Bactérias orais e biofilme;
Chitosan;
Mouthrinse;
Antimicrobial agent;
Oral bacteria;
Biofilm
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APA (6th Edition):
Vieira, L. B. (2006). Eficácia do bochecho de quitosana a 0,4% sobre o biofilme e bactérias orais
. (Thesis). Universidade do Rio Grande do Norte. Retrieved from http://repositorio.ufrn.br/handle/123456789/17033
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Vieira, Liza Barreto. “Eficácia do bochecho de quitosana a 0,4% sobre o biofilme e bactérias orais
.” 2006. Thesis, Universidade do Rio Grande do Norte. Accessed April 17, 2021.
http://repositorio.ufrn.br/handle/123456789/17033.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Vieira, Liza Barreto. “Eficácia do bochecho de quitosana a 0,4% sobre o biofilme e bactérias orais
.” 2006. Web. 17 Apr 2021.
Vancouver:
Vieira LB. Eficácia do bochecho de quitosana a 0,4% sobre o biofilme e bactérias orais
. [Internet] [Thesis]. Universidade do Rio Grande do Norte; 2006. [cited 2021 Apr 17].
Available from: http://repositorio.ufrn.br/handle/123456789/17033.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Vieira LB. Eficácia do bochecho de quitosana a 0,4% sobre o biofilme e bactérias orais
. [Thesis]. Universidade do Rio Grande do Norte; 2006. Available from: http://repositorio.ufrn.br/handle/123456789/17033
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
30.
Topchyan, Paytsar.
Adjuvant therapy with probiotic bacteria increases in vivo survival and function of natural killer cells in humanized mice.
Degree: Oral Biology, 2016, UCLA
URL: http://www.escholarship.org/uc/item/1nv1v08s
► Natural killer cells target and kill cancer stem cells (CSCs)/undifferentiated tumors, as well as healthy, non-transformed stem cells. Following selection, NK cells differentiate CSCs, via…
(more)
▼ Natural killer cells target and kill cancer stem cells (CSCs)/undifferentiated tumors, as well as healthy, non-transformed stem cells. Following selection, NK cells differentiate CSCs, via secreted and membrane-bound IFN-γ and TNF-α. Probiotic bacteria increase the cytokine secretion function of split anergized NK cells, causing significant induction of IFN-γ. Thus, treatment of NK cells with probiotic bacteria induces differentiation of CSCs. Additionally, probiotic bacteria, in combination with osteoclasts maintain and expand highly functional NK cells for a long period of time. This novel method of expanding a large number of highly functional NK cells may be a breakthrough strategy for adoptive NK immunotherapy. In this study, NK immunotherapy was studied in combination with supplementation of probiotic bacteria in the humanized mouse model. NK immunotherapy increased cytokine production in immune tissue of subjects, while probiotic supplementation further enhanced these effects, resulting in more differentiated tumors in vivo.
Subjects/Keywords: Immunology; Medicine; Biology; cancer stem cells; humanized mice; immunotherapy; natural killer cells; oral cancer; probiotic bacteria
…differentiation.
Probiotic bacteria
In the early 20th century, Elie Metchnikoff discovered that certain… …strains of bacteria in
the human gut were beneficial to gut homeostasis; these beneficial… …bacteria were named
probiotics [25]. Probiotics are commonly used in foods and… …consist of lactic-acid producing bacteria,
including lactobacilli, streptococci, and… …bifidobacteria. The wide range of benefits observed with
the use of probiotic bacteria suggests their…
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APA (6th Edition):
Topchyan, P. (2016). Adjuvant therapy with probiotic bacteria increases in vivo survival and function of natural killer cells in humanized mice. (Thesis). UCLA. Retrieved from http://www.escholarship.org/uc/item/1nv1v08s
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Topchyan, Paytsar. “Adjuvant therapy with probiotic bacteria increases in vivo survival and function of natural killer cells in humanized mice.” 2016. Thesis, UCLA. Accessed April 17, 2021.
http://www.escholarship.org/uc/item/1nv1v08s.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Topchyan, Paytsar. “Adjuvant therapy with probiotic bacteria increases in vivo survival and function of natural killer cells in humanized mice.” 2016. Web. 17 Apr 2021.
Vancouver:
Topchyan P. Adjuvant therapy with probiotic bacteria increases in vivo survival and function of natural killer cells in humanized mice. [Internet] [Thesis]. UCLA; 2016. [cited 2021 Apr 17].
Available from: http://www.escholarship.org/uc/item/1nv1v08s.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Topchyan P. Adjuvant therapy with probiotic bacteria increases in vivo survival and function of natural killer cells in humanized mice. [Thesis]. UCLA; 2016. Available from: http://www.escholarship.org/uc/item/1nv1v08s
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
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