subject:(oogenesis)

Tulane University

1. Crawford, Lydia. Origin, Form, and Function of Follicle Cell Processes in Chondrichthyan Fishes.

Degree: 2020, Tulane University

URL: https://digitallibrary.tulane.edu/islandora/object/tulane:120438

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In this dissertation, I characterize the origins, phylogenetic distribution, and function of Follicle Cell Processes (FCP) in Class Chondrichthyes. FCP are actin associated tubes… (more)

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[email protected]

In this dissertation, I characterize the origins, phylogenetic distribution, and function of Follicle Cell Processes (FCP) in Class Chondrichthyes. FCP are actin associated tubes that have been detected in the intercellular space between a developing oocyte and the surrounding follicle cells in chondrichthyan fishes. Using light microscopy, I describe the presence FCP in Hydrolagus colliei, a species of Subclass Holocephali, a divergent lineage at the base of the chondrichthyan phylogeny. FCP were also detected in species representing six of nine studied orders of Subclass Elasmobranchii, the sister group of holocephalans, but absent in three examined batoid orders. To establish the role FCP play in vitellogenesis - the process of providing yolk to developing oocytes - I performed fluorescent microscopy, staining specifically for myosin, an actin-associated transport protein previously identified in vitellogenic oocytes. The detection of myosin in oocytes during vitellogenesis and its association with actin in FCP are inconclusive. I performed RNA sequencing on follicles and ovarian tissues from Mustelus sinusmexicanis, using Illumina Hi-Seq 4000 profiling with the specific aim of identifying the types of myosins and actins involved in yolk transport. Three (MYH9, MYH10, and MYO5A) of particular interest and one actin (ACTG1L) were differentially expressed in the tissues. MYO5A is a class V myosin that has been associated with cytoplasmic vesicle transport, though it is most abundant in melanocytes and nerve cells. MYH9 and MYH10 are membrane associated non-muscle class II myosin paralogs, and MYH9 was upregulated in the ovarian tissues; MYH10 was upregulated in the corresponding follicle tissues. ACTG1L, the only actin transcript differentially expressed codes for a gamma actin isoform involved in internal cell mobility.

1

Lydia Crawford

Advisors/Committee Members: Bart, Henry Jr. (Thesis advisor), School of Science & Engineering Ecology and Evolutionary Biology (Degree granting institution), NULL (Degree granting institution).

Subjects/Keywords: Oogenesis

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APA (6^th Edition):

Crawford, L. (2020). Origin, Form, and Function of Follicle Cell Processes in Chondrichthyan Fishes. (Thesis). Tulane University. Retrieved from https://digitallibrary.tulane.edu/islandora/object/tulane:120438

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Crawford, Lydia. “Origin, Form, and Function of Follicle Cell Processes in Chondrichthyan Fishes.” 2020. Thesis, Tulane University. Accessed January 22, 2021. https://digitallibrary.tulane.edu/islandora/object/tulane:120438.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Crawford, Lydia. “Origin, Form, and Function of Follicle Cell Processes in Chondrichthyan Fishes.” 2020. Web. 22 Jan 2021.

Vancouver:

Crawford L. Origin, Form, and Function of Follicle Cell Processes in Chondrichthyan Fishes. [Internet] [Thesis]. Tulane University; 2020. [cited 2021 Jan 22]. Available from: https://digitallibrary.tulane.edu/islandora/object/tulane:120438.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Crawford L. Origin, Form, and Function of Follicle Cell Processes in Chondrichthyan Fishes. [Thesis]. Tulane University; 2020. Available from: https://digitallibrary.tulane.edu/islandora/object/tulane:120438

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Oregon State University

2. Chatlynne, Louise Geller. An ultrastructure study of oogenesis in the sea urchin, Strongylocentrotus purpuratus.

Degree: PhD, Zoology, 1971, Oregon State University

URL: http://hdl.handle.net/1957/46068

► Oogenesis in the sea urchin Strongylocentrotus purpuratus was studied at the fine structural level, with special emphasis on the relationship between the two main cell… (more)

▼ Oogenesis in the sea urchin Strongylocentrotus purpuratus was studied at the fine structural level, with special emphasis on the relationship between the two main cell types within the ovary: the egg cells and the nutritive phagocytes, or accessory cells. The nutritive phagocytes are flagellated cells with extensive pseudopods which store nutrients that are supplied to the growing oocytes and which also phagocytize degenerating eggs and cellular debris. Several aldehyde fixatives at various pH were compared with the best overall results obtained from formaldehyde-gluteraldehyde with a phosphate buffer at pH 7.0 and 0.45 M sucrose followed by osmium postfixation. Oogenesis can be divided roughly into several stages, characterized by certain structural features. The oogonial phase eggs are about 5 μm in diameter and occur in tightly packed clumps near the ovarian wall. During synapsis the chromosomes become very dense and synaptenemal complexes are obvious. In the post synaptic phase, the cytoplasm of the oocyte becomes much denser, large invaginations occur in the oolema, and the number of pores in the nuclear membrane increases. As the oocyte enters the premicrovilli growth phase, it separates from the oogonial clump and becomes surrounded by the nutritive phagocytes or their pseudopods. Yolk and cortical granule formation begins early in this period. Before any Golgi complexes are seen, a finely granular, membrane bound yolk is formed. Later, after Golgi complexes are apparent, a second type of yolk, the yolk platelet, is formed which is made up of subunits and becomes much more numerous than the granular yolk. During this phase the oolema forms narrow invaginations and bulbous pseudopods. As the elongated egg reaches a size of roughly 15 x 30 μm, finger-like extentions, for which the microvilli growth phase is named, appear on the surface. Often these microvilli interdigitate with pseudopods of the nutritive phagocytes. Bordered pits indicative of pinocytosis can be seen on the surface of the oocytes, while large membrane-bound vesicles containing amorphous material aggregate in the cytoplasm near the oolema. Golgi complexes appear more compact as the egg attains its final size of 60 - 70 μm in diameter. During the period of transition from oocyte to ovum, the eggs separate from the nutritive phagocytes and their microvilli become very elongated. With the breakdown of the germinal vesicle the microvilli become shorter, but the cortical granules remain randomly scattered in the cytoplasm. The ovum is characterized by the presence of the pronucleus and cortical granules at its surface, the appearance of heavy bodies and yolk nuclei in the cytoplasm, and formation of the vitelline membrane. Two types of ova can be distinguished in the ovary, based on their difference in electron density. Advisors/Committee Members: Harris, Patricia (advisor).

Subjects/Keywords: Oogenesis

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APA (6^th Edition):

Chatlynne, L. G. (1971). An ultrastructure study of oogenesis in the sea urchin, Strongylocentrotus purpuratus. (Doctoral Dissertation). Oregon State University. Retrieved from http://hdl.handle.net/1957/46068

Chicago Manual of Style (16^th Edition):

Chatlynne, Louise Geller. “An ultrastructure study of oogenesis in the sea urchin, Strongylocentrotus purpuratus.” 1971. Doctoral Dissertation, Oregon State University. Accessed January 22, 2021. http://hdl.handle.net/1957/46068.

MLA Handbook (7^th Edition):

Chatlynne, Louise Geller. “An ultrastructure study of oogenesis in the sea urchin, Strongylocentrotus purpuratus.” 1971. Web. 22 Jan 2021.

Vancouver:

Chatlynne LG. An ultrastructure study of oogenesis in the sea urchin, Strongylocentrotus purpuratus. [Internet] [Doctoral dissertation]. Oregon State University; 1971. [cited 2021 Jan 22]. Available from: http://hdl.handle.net/1957/46068.

Council of Science Editors:

Chatlynne LG. An ultrastructure study of oogenesis in the sea urchin, Strongylocentrotus purpuratus. [Doctoral Dissertation]. Oregon State University; 1971. Available from: http://hdl.handle.net/1957/46068

Oregon State University

3. Elvin, David Winter. Oogenesis in Mytilus californianus.

Degree: PhD, Oceanography, 1974, Oregon State University

URL: http://hdl.handle.net/1957/28666

► A two-year field study was made of a population of the California Sea Mussel, Mytilus californianus, located on a protected tidal bench at Yaquina Head… (more)

▼ A two-year field study was made of a population of the California Sea Mussel, Mytilus californianus, located on a protected tidal bench at Yaquina Head on the Central Oregon Coast. This animal was chosen because it is attached to the rocks, filters particulate matter from the water, produces large amounts of eggs, and is a prominent member of the intertidal community. An equation was developed on both an empirical and a theoretical has is for determining mussel tissue temperature from continuously, recorded physical data in order to determine the annual temperature trends and the daily rate of heating. Heating during exposure is an important contribution to mussel temperature during the spring and possibly during the fall. Particulate organic material 100 to 200 m in diameter was measured throughout the year and was found to range from 1 to 3 mg dry weight per liter of sea water. During the spring, increases in particulate oxidizable material are associated with diatom blooms. The rest of the year, detritus associated with the mixing action of waves contributes a significant portion to the suspended particulates. A gonad index was developed which separated the germinal tissue from the storage tissue in the gonad. The denominator of this index equation was the weight of the somatic tissue rather than the total weight of the animal. The largest amount of reproductive tissue observed was 800 mg per gram dry weight of somatic tissue, but the population generally maintained a germinal gonad index of about 60% of its potential maximum. The storage stage was found to be greatly reduced in this population unlike that found in M. edulis. The seasonal reproductive progress was studied by quantitatively following three categories of oocyte stages. The number of oogonial clusters was used as an index of mitotic activity. Few clusters of oogonia appeared when numerous mature oocytes were present. The number of previtellogenic oocytes was taken as an index of early meiotic activity. Previtellogenic processes were initiated during the spring when the tissue temperature was rising. Mature oocyte numbers increased as vitellogenesis occurred, and the rate of increase of mature oocytes was best correlated with particulate organic concentration in the water. Partial spawning and resorption of lysed eggs were the predominant fate of mature eggs. The dynamic aspect of oogenesis was followed using a flow model for the categories of occyte stages. From this model rates of change of each cell type were estimated. Only 40% of the total oocytes produced throughout the year was lost by spawning. Gonadal tissue was chemically divided into biochemical fractions to determine the chemical characteristics of each reproductive stage and the rates of accumulation of chemical components between bimonthly samples. The rates of accumulation of protein and lipid increased from 1.7 to 3.6 mg per day per gram animal and 0.6 to 1.8 mg per day per gram animal during previtellogenesis and vitellogenesis respectively.… Advisors/Committee Members: Gonor, J. J. (advisor).

Subjects/Keywords: Oogenesis

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APA (6^th Edition):

Elvin, D. W. (1974). Oogenesis in Mytilus californianus. (Doctoral Dissertation). Oregon State University. Retrieved from http://hdl.handle.net/1957/28666

Chicago Manual of Style (16^th Edition):

Elvin, David Winter. “Oogenesis in Mytilus californianus.” 1974. Doctoral Dissertation, Oregon State University. Accessed January 22, 2021. http://hdl.handle.net/1957/28666.

MLA Handbook (7^th Edition):

Elvin, David Winter. “Oogenesis in Mytilus californianus.” 1974. Web. 22 Jan 2021.

Vancouver:

Elvin DW. Oogenesis in Mytilus californianus. [Internet] [Doctoral dissertation]. Oregon State University; 1974. [cited 2021 Jan 22]. Available from: http://hdl.handle.net/1957/28666.

Council of Science Editors:

Elvin DW. Oogenesis in Mytilus californianus. [Doctoral Dissertation]. Oregon State University; 1974. Available from: http://hdl.handle.net/1957/28666

Oregon State University

4. Chatlynne, Louise Geller. A histochemical study of oogenesis in the sea urchin, Strogylocentrotus purpuratus.

Degree: MA, Zoology, 1968, Oregon State University

URL: http://hdl.handle.net/1957/47136

► Oögenesis in the sea urchin Strongylocentrotus purpuratus was studied by histological methods and by histochemical techniques for polysaccharides, lipids, and nucleic acids Urchins were collected… (more)

▼ Oögenesis in the sea urchin Strongylocentrotus purpuratus was studied by histological methods and by histochemical techniques for polysaccharides, lipids, and nucleic acids Urchins were collected at Yaquina Head, Oregon at regular intervals between April 1966 and March 1967. An attempt was made to correlate seasonal variations in the coastal water temperature with the gonadal cycle. The ovaries of the sea urchin are large rebranched sacs covered with a flagellated peritoneal epithelium. Inside the peritoneum is a wall of collagenous connective tissue and smooth muscle. In the central portion of each saccule or acinus of the ovary are two main cell types: the sex cells, which develop into mature ova, and the accessory cells or nutritive phagocytes which apparently provide nutriment for the sex cells. Oögonia can be found through out the year in small groups scattered along the walls of the ovary, but are most numerous in the late spring and early summer when the ovary is spent. The oöcytes start growing in the late summer and early fall when the accessory cells, which were depleted of nutriments in the spent ovary, start filling with lipid and polysaccharide globules. At this time the accessory cells are also found to have inclusions that appear to be degenerate sex cells. In the late fall and early winter, the oöcytes continue to grow and their cytoplasm fills with lipid and polysaccharides. As the ova mature they move from the walls to the central portion of the acinus where they displace the accessory cells that had formerly been there. The ova that have been shed or are about to be shed contain pyranophilic RNA which is not found in the oöcytes. However, both ova and oöcytes have RNA that is stainable with azure B. The pyranophilic RNA is also found in accessory cells. Since all the oöcytes do not mature at the same time, a sea urchin is able to shed many times during the breeding season which lasts from late winter to early spring, During this period the accessory cells progressively lose their globules. When the accessory cells are finally depleted of their lipid and polysaccharide, the öócytes no longer grow and the ovaries are spent. Advisors/Committee Members: Harris, Patricia J. (advisor).

Subjects/Keywords: Oogenesis

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Chatlynne, L. G. (1968). A histochemical study of oogenesis in the sea urchin, Strogylocentrotus purpuratus. (Masters Thesis). Oregon State University. Retrieved from http://hdl.handle.net/1957/47136

Chicago Manual of Style (16^th Edition):

Chatlynne, Louise Geller. “A histochemical study of oogenesis in the sea urchin, Strogylocentrotus purpuratus.” 1968. Masters Thesis, Oregon State University. Accessed January 22, 2021. http://hdl.handle.net/1957/47136.

MLA Handbook (7^th Edition):

Chatlynne, Louise Geller. “A histochemical study of oogenesis in the sea urchin, Strogylocentrotus purpuratus.” 1968. Web. 22 Jan 2021.

Vancouver:

Chatlynne LG. A histochemical study of oogenesis in the sea urchin, Strogylocentrotus purpuratus. [Internet] [Masters thesis]. Oregon State University; 1968. [cited 2021 Jan 22]. Available from: http://hdl.handle.net/1957/47136.

Council of Science Editors:

Chatlynne LG. A histochemical study of oogenesis in the sea urchin, Strogylocentrotus purpuratus. [Masters Thesis]. Oregon State University; 1968. Available from: http://hdl.handle.net/1957/47136

University of Manchester

5. Ford, Nicola. Characterisation of genes involved in early oogenesis in Drosophila melanogaster.

Degree: 2013, University of Manchester

URL: http://www.manchester.ac.uk/escholar/uk-ac-man-scw:189898

► Tissue maintenance requires a balance between cell production and cell death. The former is dependent on the activity of stem cells, which in turn are… (more)

Subjects/Keywords: Oogenesis; Tissue maintenance

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Ford, N. (2013). Characterisation of genes involved in early oogenesis in Drosophila melanogaster. (Doctoral Dissertation). University of Manchester. Retrieved from http://www.manchester.ac.uk/escholar/uk-ac-man-scw:189898

Chicago Manual of Style (16^th Edition):

Ford, Nicola. “Characterisation of genes involved in early oogenesis in Drosophila melanogaster.” 2013. Doctoral Dissertation, University of Manchester. Accessed January 22, 2021. http://www.manchester.ac.uk/escholar/uk-ac-man-scw:189898.

MLA Handbook (7^th Edition):

Ford, Nicola. “Characterisation of genes involved in early oogenesis in Drosophila melanogaster.” 2013. Web. 22 Jan 2021.

Vancouver:

Ford N. Characterisation of genes involved in early oogenesis in Drosophila melanogaster. [Internet] [Doctoral dissertation]. University of Manchester; 2013. [cited 2021 Jan 22]. Available from: http://www.manchester.ac.uk/escholar/uk-ac-man-scw:189898.

Council of Science Editors:

Ford N. Characterisation of genes involved in early oogenesis in Drosophila melanogaster. [Doctoral Dissertation]. University of Manchester; 2013. Available from: http://www.manchester.ac.uk/escholar/uk-ac-man-scw:189898

University of Manchester

6. Ford, Nicola. Characterisation of genes involved in early oogenesis in Drosophila melanogaster.

Degree: PhD, 2013, University of Manchester

URL: https://www.research.manchester.ac.uk/portal/en/theses/characterisation-of-genes-involved-in-early-oogenesis-in-drosophila-melanogaster(05b6edbb-f496-47a8-aa80-5bd238d374f1).html ; https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.740244

► Tissue maintenance requires a balance between cell production and cell death. The former is dependent on the activity of stem cells, which in turn are… (more)

Subjects/Keywords: 595.77; Oogenesis; Tissue maintenance

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Ford, N. (2013). Characterisation of genes involved in early oogenesis in Drosophila melanogaster. (Doctoral Dissertation). University of Manchester. Retrieved from https://www.research.manchester.ac.uk/portal/en/theses/characterisation-of-genes-involved-in-early-oogenesis-in-drosophila-melanogaster(05b6edbb-f496-47a8-aa80-5bd238d374f1).html ; https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.740244

Chicago Manual of Style (16^th Edition):

Ford, Nicola. “Characterisation of genes involved in early oogenesis in Drosophila melanogaster.” 2013. Doctoral Dissertation, University of Manchester. Accessed January 22, 2021. https://www.research.manchester.ac.uk/portal/en/theses/characterisation-of-genes-involved-in-early-oogenesis-in-drosophila-melanogaster(05b6edbb-f496-47a8-aa80-5bd238d374f1).html ; https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.740244.

MLA Handbook (7^th Edition):

Ford, Nicola. “Characterisation of genes involved in early oogenesis in Drosophila melanogaster.” 2013. Web. 22 Jan 2021.

Vancouver:

Ford N. Characterisation of genes involved in early oogenesis in Drosophila melanogaster. [Internet] [Doctoral dissertation]. University of Manchester; 2013. [cited 2021 Jan 22]. Available from: https://www.research.manchester.ac.uk/portal/en/theses/characterisation-of-genes-involved-in-early-oogenesis-in-drosophila-melanogaster(05b6edbb-f496-47a8-aa80-5bd238d374f1).html ; https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.740244.

Council of Science Editors:

Ford N. Characterisation of genes involved in early oogenesis in Drosophila melanogaster. [Doctoral Dissertation]. University of Manchester; 2013. Available from: https://www.research.manchester.ac.uk/portal/en/theses/characterisation-of-genes-involved-in-early-oogenesis-in-drosophila-melanogaster(05b6edbb-f496-47a8-aa80-5bd238d374f1).html ; https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.740244

University of Toronto

7. Hwang, Michael Shang-Hsien. Analysis of Sequence and Function of Drosophila Microvillus Cadherins, Cad74A, Cad87A, and Cad88C.

Degree: 2011, University of Toronto

URL: http://hdl.handle.net/1807/29562

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Microvilli are actin-based protrusions at the apical surface of epithelial cells and have diverse functions. My bioinformatic analysis suggests that human Cadherin 23, which is… (more)

Subjects/Keywords: Drosophila; Microvillus; Cadherins; Oogenesis; 0379

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APA (6^th Edition):

Hwang, M. S. (2011). Analysis of Sequence and Function of Drosophila Microvillus Cadherins, Cad74A, Cad87A, and Cad88C. (Masters Thesis). University of Toronto. Retrieved from http://hdl.handle.net/1807/29562

Chicago Manual of Style (16^th Edition):

Hwang, Michael Shang-Hsien. “Analysis of Sequence and Function of Drosophila Microvillus Cadherins, Cad74A, Cad87A, and Cad88C.” 2011. Masters Thesis, University of Toronto. Accessed January 22, 2021. http://hdl.handle.net/1807/29562.

MLA Handbook (7^th Edition):

Hwang, Michael Shang-Hsien. “Analysis of Sequence and Function of Drosophila Microvillus Cadherins, Cad74A, Cad87A, and Cad88C.” 2011. Web. 22 Jan 2021.

Vancouver:

Hwang MS. Analysis of Sequence and Function of Drosophila Microvillus Cadherins, Cad74A, Cad87A, and Cad88C. [Internet] [Masters thesis]. University of Toronto; 2011. [cited 2021 Jan 22]. Available from: http://hdl.handle.net/1807/29562.

Council of Science Editors:

Hwang MS. Analysis of Sequence and Function of Drosophila Microvillus Cadherins, Cad74A, Cad87A, and Cad88C. [Masters Thesis]. University of Toronto; 2011. Available from: http://hdl.handle.net/1807/29562

University of Wisconsin – La Cross

8. Keding, Logan T. Effects of drug-induced hepatotoxicity on vitellogenesis in the fathead minnow (Pimephales promelas).

Degree: 2019, University of Wisconsin – La Cross

URL: http://digital.library.wisc.edu/1793/79383

► The vitellogenin (VTG) assay is a test used to identify estrogen axis endocrine disruptors (EDs) in fish species. The VTG assay has been seemingly successful,… (more)

Subjects/Keywords: Hepatotoxicology; Oogenesis; Fathead minnow; Biology

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APA (6^th Edition):

Keding, L. T. (2019). Effects of drug-induced hepatotoxicity on vitellogenesis in the fathead minnow (Pimephales promelas). (Thesis). University of Wisconsin – La Cross. Retrieved from http://digital.library.wisc.edu/1793/79383

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Keding, Logan T. “Effects of drug-induced hepatotoxicity on vitellogenesis in the fathead minnow (Pimephales promelas).” 2019. Thesis, University of Wisconsin – La Cross. Accessed January 22, 2021. http://digital.library.wisc.edu/1793/79383.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Keding, Logan T. “Effects of drug-induced hepatotoxicity on vitellogenesis in the fathead minnow (Pimephales promelas).” 2019. Web. 22 Jan 2021.

Vancouver:

Keding LT. Effects of drug-induced hepatotoxicity on vitellogenesis in the fathead minnow (Pimephales promelas). [Internet] [Thesis]. University of Wisconsin – La Cross; 2019. [cited 2021 Jan 22]. Available from: http://digital.library.wisc.edu/1793/79383.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Keding LT. Effects of drug-induced hepatotoxicity on vitellogenesis in the fathead minnow (Pimephales promelas). [Thesis]. University of Wisconsin – La Cross; 2019. Available from: http://digital.library.wisc.edu/1793/79383

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Cambridge

9. Plygawko, Andrew Thomas. Investigating the underlying mechanisms of axis formation in Drosophila melanogaster.

Degree: PhD, 2020, University of Cambridge

URL: https://www.repository.cam.ac.uk/handle/1810/302760

► The establishment of the body axes has been well-studied in Drosophila melanogaster, and is known to occur prior to fertilisation in the developing egg chamber… (more)

▼ The establishment of the body axes has been well-studied in Drosophila melanogaster, and is known to occur prior to fertilisation in the developing egg chamber as a result of crosstalk between the germline and the surrounding somatic epithelium. Whilst it is known that the formation of the anterior-posterior and dorsal-ventral axes occurs due to the establishment of oocyte cell polarity and repositioning of the oocyte nucleus, respectively, the nature of the signals sent from the soma to trigger these two events, and the method through which these signals are transduced, remain unclear. In an attempt to elucidate these underlying mechanisms, studies of both the follicle epithelium and germline were undertaken. Posterior follicle cells are known to be responsible for the formation of both body axes through signalling, but very few factors have been identified which perturb this process. The transcriptomic method Targeted DamID was used to quantify gene expression in these cells, compared to their lateral follicle cells counterparts. This resulted in the identification of 140 genes which were either uniquely expressed or upregulated in posterior follicle cells. Specific subsets of these genes were knocked down via RNAi, and the impact of this knockdown on axis formation determined. However, an attempted validation of these observed phenotypes through CRISPR-based mutagenesis or analysis of extant alleles failed to recapitulate many of the expected defects. Subsequent experiments have attempted to further narrow this list of differentially-expressed genes to provide a more specific profile of the posterior follicle cell transcriptome. In parallel, a forward genetic screen was undertaken to identify novel factors required for axis formation in the germline. Whole genome sequencing of a mutant which fails to establish cell polarity identified the causative mutation as a SNP in the enzyme CDP-diacylglycerol synthase (CdsA), required for the production of a number of phospholipid species. An initial characterisation of this mutant phenotype suggested the activity of CdsA is required to recruit the polarity kinase Par-1 to the posterior of the oocyte. A further analysis established that the production of phosphatidylglycerol-based species is dispensable for oocyte polarity establishment, suggesting that the phenotype of cdsa alleles is produced due to its impact on phosphatidylinositol synthesis. Additionally, an analysis of the localisation of the PIP2 derivative diacylglycerol using a fluorescent biosensor revealed its localisation was not uniform within the oocyte. To better understand potential downstream functions of oocyte cell polarity in the context of axis formation, several previously-identified Par-1 phosphorylation target sites were examined. The six sites of interest were located in the eIF4E-binding protein Cup, which is required to repress translation of osk mRNA until it is posteriorly-localised. To analyse the function of these phosphorylation sites, several Cup-GFP transgenes expressed under the endogenous…

Subjects/Keywords: Oogenesis; Drosophila; Cell polarity

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APA (6^th Edition):

Plygawko, A. T. (2020). Investigating the underlying mechanisms of axis formation in Drosophila melanogaster. (Doctoral Dissertation). University of Cambridge. Retrieved from https://www.repository.cam.ac.uk/handle/1810/302760

Chicago Manual of Style (16^th Edition):

Plygawko, Andrew Thomas. “Investigating the underlying mechanisms of axis formation in Drosophila melanogaster.” 2020. Doctoral Dissertation, University of Cambridge. Accessed January 22, 2021. https://www.repository.cam.ac.uk/handle/1810/302760.

MLA Handbook (7^th Edition):

Plygawko, Andrew Thomas. “Investigating the underlying mechanisms of axis formation in Drosophila melanogaster.” 2020. Web. 22 Jan 2021.

Vancouver:

Plygawko AT. Investigating the underlying mechanisms of axis formation in Drosophila melanogaster. [Internet] [Doctoral dissertation]. University of Cambridge; 2020. [cited 2021 Jan 22]. Available from: https://www.repository.cam.ac.uk/handle/1810/302760.

Council of Science Editors:

Plygawko AT. Investigating the underlying mechanisms of axis formation in Drosophila melanogaster. [Doctoral Dissertation]. University of Cambridge; 2020. Available from: https://www.repository.cam.ac.uk/handle/1810/302760

University of Montana

10. Negherbon, William Ovide. An examination of ovogenesis in the cestode Taenia pisiformis.

Degree: MA, 1935, University of Montana

URL: https://scholarworks.umt.edu/etd/6728

Subjects/Keywords: Oogenesis.; Tapeworms.

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APA (6^th Edition):

Negherbon, W. O. (1935). An examination of ovogenesis in the cestode Taenia pisiformis. (Masters Thesis). University of Montana. Retrieved from https://scholarworks.umt.edu/etd/6728

Chicago Manual of Style (16^th Edition):

Negherbon, William Ovide. “An examination of ovogenesis in the cestode Taenia pisiformis.” 1935. Masters Thesis, University of Montana. Accessed January 22, 2021. https://scholarworks.umt.edu/etd/6728.

MLA Handbook (7^th Edition):

Negherbon, William Ovide. “An examination of ovogenesis in the cestode Taenia pisiformis.” 1935. Web. 22 Jan 2021.

Vancouver:

Negherbon WO. An examination of ovogenesis in the cestode Taenia pisiformis. [Internet] [Masters thesis]. University of Montana; 1935. [cited 2021 Jan 22]. Available from: https://scholarworks.umt.edu/etd/6728.

Council of Science Editors:

Negherbon WO. An examination of ovogenesis in the cestode Taenia pisiformis. [Masters Thesis]. University of Montana; 1935. Available from: https://scholarworks.umt.edu/etd/6728

University of Texas – Austin

11. Pace, Margaret Courtney. Molecular mechanisms regulating oocyte maturation in sciaenid fishes.

Degree: PhD, Marine Science, 2005, University of Texas – Austin

URL: http://hdl.handle.net/2152/2030

► The overall aim of this research was to study the molecular mechanisms regulating oocyte maturation in sciaenid fishes using two models, the Atlantic croaker and… (more)

▼ The overall aim of this research was to study the molecular mechanisms regulating oocyte maturation in sciaenid fishes using two models, the Atlantic croaker and the spotted seatrout. The steroid receptor mediating oocyte maturation in these species, as well as the signal transduction pathways altered by binding of the maturation inducing steroid 17,20β,21-trihydroxy-4-pregene-3-one (20β-S) to the oocyte were investigated. Finally, a cDNA of the gene encoding the membrane progestin receptor, mPRα, was isolated from Atlantic croaker ovary and hormonal regulation of its mRNA and protein expression was examined. The 20β-S receptor in seatrout oocytes was shown to be a novel steroid hormone receptor located in oocyte membranes that was directly coupled to a pertussis toxin sensitive G-protein. Activation of this protein was found to be necessary for oocyte maturation in Atlantic croaker and seatrout. Support for a role for the novel G-protein coupled progestin binding receptor, mPRα, in spotted seatrout oocyte maturation was presented. Multiple signal transduction pathways were activated by 20β-S binding to its receptor in Atlantic croaker oocyte membranes. Treatment of oocyte membranes with 20β-S significantly decreased adenylyl cyclase activity within 1 minute of exposure, although inhibition of cAMP-dependent protein kinase activity was not sufficient to induce oocyte maturation in the absence of steroid. Activation of phosphatidylinositol-3-kinase and Akt was necessary for 20β-S-induced oocyte maturation in Atlantic croaker and data suggested that phosphodiesterase 3 and phosphodiesterase 4 assist in maintaining oocyte meiotic arrest. The mitogen activated protein kinase pathway in Atlantic croaker oocytes was activated within 1 hour of 20β-S treatment; however inhibition of this pathway had no effect on steroid-mediated oocyte maturation. Finally, a cDNA of the membrane progestin receptor, mPRα, was isolated from Atlantic croaker ovary. mPRα mRNA was expressed in all tissues, however its protein distribution was limited to ovary, testis, intestine, heart, gill, brain and olfactory epithelium. Treatment of Atlantic croaker ovarian follicles with gonadotropin, but not MIS, upregulated mPRα protein and inhibition of steroidogenesis had no effect on this hCG-mediated effect or on GVBD. These findings support previous studies in seatrout and support the hypothesis that mPRα is a mediator of steroid-mediated oocyte maturation. Advisors/Committee Members: Thomas, Peter (advisor).

Subjects/Keywords: Oogenesis; Sciaenidae

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Pace, M. C. (2005). Molecular mechanisms regulating oocyte maturation in sciaenid fishes. (Doctoral Dissertation). University of Texas – Austin. Retrieved from http://hdl.handle.net/2152/2030

Chicago Manual of Style (16^th Edition):

Pace, Margaret Courtney. “Molecular mechanisms regulating oocyte maturation in sciaenid fishes.” 2005. Doctoral Dissertation, University of Texas – Austin. Accessed January 22, 2021. http://hdl.handle.net/2152/2030.

MLA Handbook (7^th Edition):

Pace, Margaret Courtney. “Molecular mechanisms regulating oocyte maturation in sciaenid fishes.” 2005. Web. 22 Jan 2021.

Vancouver:

Pace MC. Molecular mechanisms regulating oocyte maturation in sciaenid fishes. [Internet] [Doctoral dissertation]. University of Texas – Austin; 2005. [cited 2021 Jan 22]. Available from: http://hdl.handle.net/2152/2030.

Council of Science Editors:

Pace MC. Molecular mechanisms regulating oocyte maturation in sciaenid fishes. [Doctoral Dissertation]. University of Texas – Austin; 2005. Available from: http://hdl.handle.net/2152/2030

University of Texas Southwestern Medical Center

12. Baker, Michael David. The Small GTPase Rheb Is Required for Spermatogenesis but not Oogenesis.

Degree: 2013, University of Texas Southwestern Medical Center

URL: http://hdl.handle.net/2152.5/2712

► The process of germ cell development is under the tight control of various signaling pathways among which the PI3K-PKB-mTOR pathway is of critical importance. Previous… (more)

Subjects/Keywords: Monomeric GTP-Binding Proteins; Neuropeptides; Oogenesis; Spermatogenesis

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Baker, M. D. (2013). The Small GTPase Rheb Is Required for Spermatogenesis but not Oogenesis. (Thesis). University of Texas Southwestern Medical Center. Retrieved from http://hdl.handle.net/2152.5/2712

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Baker, Michael David. “The Small GTPase Rheb Is Required for Spermatogenesis but not Oogenesis.” 2013. Thesis, University of Texas Southwestern Medical Center. Accessed January 22, 2021. http://hdl.handle.net/2152.5/2712.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Baker, Michael David. “The Small GTPase Rheb Is Required for Spermatogenesis but not Oogenesis.” 2013. Web. 22 Jan 2021.

Vancouver:

Baker MD. The Small GTPase Rheb Is Required for Spermatogenesis but not Oogenesis. [Internet] [Thesis]. University of Texas Southwestern Medical Center; 2013. [cited 2021 Jan 22]. Available from: http://hdl.handle.net/2152.5/2712.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Baker MD. The Small GTPase Rheb Is Required for Spermatogenesis but not Oogenesis. [Thesis]. University of Texas Southwestern Medical Center; 2013. Available from: http://hdl.handle.net/2152.5/2712

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Toronto

13. Draginov, Arman George. Okapi (Oka), a Novel FERM Domain Protein Essential for Drosophila Oogenesis.

Degree: 2014, University of Toronto

URL: http://hdl.handle.net/1807/82605

►

FERM proteins are a class of important adaptors in animal tissues. They are ubiquitous in the cytocortex, linking the plasma membrane to cytoskeletal components, and… (more)

Subjects/Keywords: Crumbs; Drosophila; Epithelia; FERM; Oogenesis; Ovary; 0307

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APA (6^th Edition):

Draginov, A. G. (2014). Okapi (Oka), a Novel FERM Domain Protein Essential for Drosophila Oogenesis. (Masters Thesis). University of Toronto. Retrieved from http://hdl.handle.net/1807/82605

Chicago Manual of Style (16^th Edition):

Draginov, Arman George. “Okapi (Oka), a Novel FERM Domain Protein Essential for Drosophila Oogenesis.” 2014. Masters Thesis, University of Toronto. Accessed January 22, 2021. http://hdl.handle.net/1807/82605.

MLA Handbook (7^th Edition):

Draginov, Arman George. “Okapi (Oka), a Novel FERM Domain Protein Essential for Drosophila Oogenesis.” 2014. Web. 22 Jan 2021.

Vancouver:

Draginov AG. Okapi (Oka), a Novel FERM Domain Protein Essential for Drosophila Oogenesis. [Internet] [Masters thesis]. University of Toronto; 2014. [cited 2021 Jan 22]. Available from: http://hdl.handle.net/1807/82605.

Council of Science Editors:

Draginov AG. Okapi (Oka), a Novel FERM Domain Protein Essential for Drosophila Oogenesis. [Masters Thesis]. University of Toronto; 2014. Available from: http://hdl.handle.net/1807/82605

University of Toronto

14. Panchal, Trupti. Traffic Jam, a Large Maf Transcription Factor, Regulates the Formation of the Germline Stem Cell Niche in the Ovary of Drosophila melanogaster.

Degree: 2014, University of Toronto

URL: http://hdl.handle.net/1807/70176

►

Cap cells form a niche for 2-3 germline stem cells (GSCs) in a Drosophila ovariole. Cap cells are organised in a cluster at the base… (more)

Subjects/Keywords: oogenesis; germline stem cell niche; 0379

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APA (6^th Edition):

Panchal, T. (2014). Traffic Jam, a Large Maf Transcription Factor, Regulates the Formation of the Germline Stem Cell Niche in the Ovary of Drosophila melanogaster. (Masters Thesis). University of Toronto. Retrieved from http://hdl.handle.net/1807/70176

Chicago Manual of Style (16^th Edition):

Panchal, Trupti. “Traffic Jam, a Large Maf Transcription Factor, Regulates the Formation of the Germline Stem Cell Niche in the Ovary of Drosophila melanogaster.” 2014. Masters Thesis, University of Toronto. Accessed January 22, 2021. http://hdl.handle.net/1807/70176.

MLA Handbook (7^th Edition):

Panchal, Trupti. “Traffic Jam, a Large Maf Transcription Factor, Regulates the Formation of the Germline Stem Cell Niche in the Ovary of Drosophila melanogaster.” 2014. Web. 22 Jan 2021.

Vancouver:

Panchal T. Traffic Jam, a Large Maf Transcription Factor, Regulates the Formation of the Germline Stem Cell Niche in the Ovary of Drosophila melanogaster. [Internet] [Masters thesis]. University of Toronto; 2014. [cited 2021 Jan 22]. Available from: http://hdl.handle.net/1807/70176.

Council of Science Editors:

Panchal T. Traffic Jam, a Large Maf Transcription Factor, Regulates the Formation of the Germline Stem Cell Niche in the Ovary of Drosophila melanogaster. [Masters Thesis]. University of Toronto; 2014. Available from: http://hdl.handle.net/1807/70176

University of Toronto

15. Sedra, Laura. FMRFamide-like Peptides and their Role in Reproduction in the Chagas Vector, Rhodnius prolixus.

Degree: PhD, 2016, University of Toronto

URL: http://hdl.handle.net/1807/76824

► Insect reproductive systems are tightly modulated by neurotransmitters via direct innervation, by neurohormones circulating in the haemolymph and by neuromodulators that can be released either… (more)

▼ Insect reproductive systems are tightly modulated by neurotransmitters via direct innervation, by neurohormones circulating in the haemolymph and by neuromodulators that can be released either locally or more widespread in the periphery. FMRFamide-like peptides (FLPs) are large families of neuropeptides with conserved RFamide C-termini and have been implicated in vertebrate and invertebrate neuroendocrinology. This thesis examines the differing roles that members of the FLP superfamily have in the adult female reproductive system of Rhodnius prolixus. The entire female reproductive tract is composed of different arrangements of striated muscles. Two members of the N-terminally extended FM/L/IRFamides have been shown to stimulate ovariole, ovary, oviduct and bursal muscle contraction in a dose-dependent manner; whereas the myosuppressin, RhoprMS, does not have any myogenic effect on the reproductive tract. The RhoprNPF (neuropeptide F) and RhoprNPF receptor (RhoprNPFR) genes have been cloned from the R. prolixus central nervous system (CNS) and phylogenetic analysis implies possible receptor-ligand co-evolution. RhoprNPFR has been classified as a G-protein coupled receptor (GPCR) containing 7 transmembrane domains and a conserved 8th Îą-helix that are classic characteristics of rhodopsin-type GPCRs. FMRFamide-like immunoreactivity (FLI) was observed in cells and processes in the adult CNS and processes on the reproductive tract. Moreover a specific subset of median neurosecretory cells (MNSCs) in the brain as well as small cell bodies along the muscle fibers of the lateral oviduct express the RhoprNPF transcript. The RhoprNPFR transcript is also expressed in putative pre-follicular cells of the R. prolixus telotrophic ovariole. RhoprNPFR transcript appears to be supplied to the developing oocyte during vitellogenesis and the receptor most likely aids in the differentiation of pre-follicular cells into mature follicle cells surrounding the growing oocyte and helps regulate the supply of nutrients. Screening members of the FLP family in an egg-laying assay showed that N-terminally extended FM/L/IRFamides and short NPF stimulate oogenesis, whereas MS inhibits it. Although RhoprNPF does not have a myogenic effect on lateral oviduct muscle, I have shown that it potentially aids in ovulation. Sulfakinins exhibit no effect on egg-laying. In summary, this thesis demonstrates the importance of FLPs in the control and regulation of the female R. prolixus reproductive system. Advisors/Committee Members: Lange, Angela B, Cell and Systems Biology.

Subjects/Keywords: contraction; immunohistochemistry; insect; neuropeptide; oogenesis; reproduction; 0719

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Sedra, L. (2016). FMRFamide-like Peptides and their Role in Reproduction in the Chagas Vector, Rhodnius prolixus. (Doctoral Dissertation). University of Toronto. Retrieved from http://hdl.handle.net/1807/76824

Chicago Manual of Style (16^th Edition):

Sedra, Laura. “FMRFamide-like Peptides and their Role in Reproduction in the Chagas Vector, Rhodnius prolixus.” 2016. Doctoral Dissertation, University of Toronto. Accessed January 22, 2021. http://hdl.handle.net/1807/76824.

MLA Handbook (7^th Edition):

Sedra, Laura. “FMRFamide-like Peptides and their Role in Reproduction in the Chagas Vector, Rhodnius prolixus.” 2016. Web. 22 Jan 2021.

Vancouver:

Sedra L. FMRFamide-like Peptides and their Role in Reproduction in the Chagas Vector, Rhodnius prolixus. [Internet] [Doctoral dissertation]. University of Toronto; 2016. [cited 2021 Jan 22]. Available from: http://hdl.handle.net/1807/76824.

Council of Science Editors:

Sedra L. FMRFamide-like Peptides and their Role in Reproduction in the Chagas Vector, Rhodnius prolixus. [Doctoral Dissertation]. University of Toronto; 2016. Available from: http://hdl.handle.net/1807/76824

University of Hawaii

16. Oyama, Stanley Nobuyuki. Neuroendocrine effects on ovarian development in the crab Thalamita crenata Latreille studied in vitro.

Degree: 2009, University of Hawaii

URL: http://hdl.handle.net/10125/11993

►

Typescript.

Bibliography: leaves 67-69.

ix, 69 l illus., tables

The inhibition of ovarian maturation by a hormone produced in the eyestalk has been repeatedly demonstrated… (more)

▼

Typescript.

Bibliography: leaves 67-69.

ix, 69 l illus., tables

The inhibition of ovarian maturation by a hormone produced in the eyestalk has been repeatedly demonstrated in many species of crustaceans. However, the discovery of an ovary stimulating factor in the thoracic ganglion has been recent and confined to two species of fresh water crabs, Potamon dehaani and Paratelphusa hydrodromous. The current investigation has disclosed the presence of a similar hormonal factor in the thoracic ganglion of a littoral crab, Thalamita crenata Latreille. Studies of these two factors and their effects on the maturation process were conducted by using the organ culture method in conjunction with in vivo experimentations. In the ill vitro studies ovarian explants were cultivated in a medium composed of Pantin's crab saline and Medium 199 without serum (Baltimore Biological Laboratories). This medium was effective in promoting growth in the germinative zone and of the interstitial cells of these explants. Growth was indicated by the presence of cells undergoing cell division and by the increased number of interstitial cells. The proliferation of these cells, especially those in the germinative zone, indicated that this aspect of oocyte development in vitro was independent of any hormonal factors. Maintenance of young oocytes was demonstrated in cultivation of explants with the culture medium. However, the addition of brain extracts to the medium showed that the brain may be involved in promoting growth in these oocytes. Older oocytes undergoing cytoplasmic changes such as vesicle formation ,and yolk deposition were not maintained in the culture medium. When the medium was supplemented with an extract of the thoracic ganglion, maintenance of these oocytes, notably those undergoing yolk deposition, was demonstrated. However, further growth was not induced. Extracts of the brain did not affect the oocytes in this stage of development. Parallel in vivo studies in which extracts of the thoracic ganglia were injected into normal females revealed the presence of a stimulating factor. This factor not only maintained vitellogenesis, but also initiated it. Eyestalk replacement studies verified the presence of an inhibitory factor in the eyestalks. This inhibitor, directly or indirectly, suppressed vitellogenesis. It is concluded that an ovarian stimulating factor is found in the thoracic ganglion. This substance initiates and maintains vitellogenesis. Earlier stages in the development of the oocytes are not affected by this thoracic ganglionic factor.

Subjects/Keywords: Crabs; Oogenesis; Ovaries

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Oyama, S. N. (2009). Neuroendocrine effects on ovarian development in the crab Thalamita crenata Latreille studied in vitro. (Thesis). University of Hawaii. Retrieved from http://hdl.handle.net/10125/11993

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Oyama, Stanley Nobuyuki. “Neuroendocrine effects on ovarian development in the crab Thalamita crenata Latreille studied in vitro.” 2009. Thesis, University of Hawaii. Accessed January 22, 2021. http://hdl.handle.net/10125/11993.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Oyama, Stanley Nobuyuki. “Neuroendocrine effects on ovarian development in the crab Thalamita crenata Latreille studied in vitro.” 2009. Web. 22 Jan 2021.

Vancouver:

Oyama SN. Neuroendocrine effects on ovarian development in the crab Thalamita crenata Latreille studied in vitro. [Internet] [Thesis]. University of Hawaii; 2009. [cited 2021 Jan 22]. Available from: http://hdl.handle.net/10125/11993.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Oyama SN. Neuroendocrine effects on ovarian development in the crab Thalamita crenata Latreille studied in vitro. [Thesis]. University of Hawaii; 2009. Available from: http://hdl.handle.net/10125/11993

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Texas – Austin

17. Kanke, Matthew Robert. Characterization of oskar translational activation and the oskar RNA function.

Degree: PhD, Cell and Molecular Biology, 2014, University of Texas – Austin

URL: http://hdl.handle.net/2152/33258

► Oskar (Osk) protein is required for posterior body patterning and establishment of the germline in Drosophila. Coordination of osk mRNA localization and translational regulation ensures… (more)

▼ Oskar (Osk) protein is required for posterior body patterning and establishment of the germline in Drosophila. Coordination of osk mRNA localization and translational regulation ensures Osk protein expression is confined to the oocyte posterior. Proper expression requires repression of osk RNA during transport and activation upon localization. Once activated, osk mRNA is translated into two protein isoforms, Long and Short Osk. Here I describe an element in the 5’ end of osk mRNA that is highly conserved across multiple Drosophila species and required for osk translational activation. This 5’ element is located in a region that is also protein coding for the longer Osk isoform and assays were designed to disentangle the effects that mutations had on protein and RNA function. The 5’ element is needed for efficient Osk translation, but only in the absence of Long Osk translation from the same transcript, suggesting a redundant role. Although the 5’ element was previously implicated in a posterior-specific relief of repression, here I provide evidence that the 5’ element acts as a general enhancer of translation, independent of localization and repression. In addition to its protein coding role, osk mRNA has a non-coding function. Egg chambers lacking osk mRNA fail to form a karyosome and arrest mid-oogenesis. RNA function depends on the presence of the osk 3’ UTR in the oocyte. Here I demonstrate that osk mRNA influences distribution of regulators. In the absence of osk mRNA these regulators dissociate from ribonucleoproteins in the germ cells and accumulate in the follicle cells. I find that the osk 3’ UTR performs multiple roles contributing to RNA function. Multiple binding sites act to sequester the translational repressor Bruno in one role. Another involves sequences not bound by Bruno near the 3’ end of osk. In contrary to disruption of Bruno sequestration, which requires mutation of multiple binding sites, mutation of a single site was sufficient to disrupt RNA function. However, disruption of either role recapitulates the failure of karyosome formation and the accumulation of regulators in the follicle cells. Advisors/Committee Members: Macdonald, Paul M. (advisor), Fischer, Janice (committee member), Johnson, Arlen (committee member), Raab-Graham, Kimberly (committee member), Stein, David (committee member).

Subjects/Keywords: Translation regulation; Drosophila oogenesis; Oskar; RNA function

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Kanke, M. R. (2014). Characterization of oskar translational activation and the oskar RNA function. (Doctoral Dissertation). University of Texas – Austin. Retrieved from http://hdl.handle.net/2152/33258

Chicago Manual of Style (16^th Edition):

Kanke, Matthew Robert. “Characterization of oskar translational activation and the oskar RNA function.” 2014. Doctoral Dissertation, University of Texas – Austin. Accessed January 22, 2021. http://hdl.handle.net/2152/33258.

MLA Handbook (7^th Edition):

Kanke, Matthew Robert. “Characterization of oskar translational activation and the oskar RNA function.” 2014. Web. 22 Jan 2021.

Vancouver:

Kanke MR. Characterization of oskar translational activation and the oskar RNA function. [Internet] [Doctoral dissertation]. University of Texas – Austin; 2014. [cited 2021 Jan 22]. Available from: http://hdl.handle.net/2152/33258.

Council of Science Editors:

Kanke MR. Characterization of oskar translational activation and the oskar RNA function. [Doctoral Dissertation]. University of Texas – Austin; 2014. Available from: http://hdl.handle.net/2152/33258

Michigan State University

18. French, Allen Lee. Spermatogenesis and oogenesis in Musca domestica L.

Degree: MS, Dept. of Entomology, 1964, Michigan State University

URL: http://etd.lib.msu.edu/islandora/object/etd:9691

Subjects/Keywords: Flies; Spermatogenesis; Oogenesis

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

French, A. L. (1964). Spermatogenesis and oogenesis in Musca domestica L. (Masters Thesis). Michigan State University. Retrieved from http://etd.lib.msu.edu/islandora/object/etd:9691

Chicago Manual of Style (16^th Edition):

French, Allen Lee. “Spermatogenesis and oogenesis in Musca domestica L.” 1964. Masters Thesis, Michigan State University. Accessed January 22, 2021. http://etd.lib.msu.edu/islandora/object/etd:9691.

MLA Handbook (7^th Edition):

French, Allen Lee. “Spermatogenesis and oogenesis in Musca domestica L.” 1964. Web. 22 Jan 2021.

Vancouver:

French AL. Spermatogenesis and oogenesis in Musca domestica L. [Internet] [Masters thesis]. Michigan State University; 1964. [cited 2021 Jan 22]. Available from: http://etd.lib.msu.edu/islandora/object/etd:9691.

Council of Science Editors:

French AL. Spermatogenesis and oogenesis in Musca domestica L. [Masters Thesis]. Michigan State University; 1964. Available from: http://etd.lib.msu.edu/islandora/object/etd:9691

UCLA

19. Roth-Johnson, Elizabeth Anne. Microtubule binding by the formin Cappuccino and its implications for Drosophila oogenesis.

Degree: Molecular Biology, 2014, UCLA

URL: http://www.escholarship.org/uc/item/3g87800k

► Coordination of actin and microtubule cytoskeletal networks is required for a number of fundamental cellular processes. Formin family actin nucleators are emerging coordinators of the… (more)

▼ Coordination of actin and microtubule cytoskeletal networks is required for a number of fundamental cellular processes. Formin family actin nucleators are emerging coordinators of the actin and microtubule cytoskeletons, as they can both nucleate actin filaments and bind microtubules in vitro. To gain a more detailed mechanistic understanding of formin-microtubule interactions and formin-mediated actin-microtubule crosstalk, we studied microtubule binding by Cappuccino (Capu), a formin involved in regulating actin and microtubule organization during Drosophila oogenesis. We report that two distinct domains within Capu, FH2 and tail, work together to promote high-affinity microtubule binding. Microtubules potently inhibit Capu's actin nucleation activity but have little effect on Capu once bound to the barbed end of an elongating actin filament, supporting a model in which Capu does not simultaneously bind microtubules and assemble actin in vivo. After characterizing Capu-microtubule binding in vitro, we examined the localization of Capu and microtubules in S2 cells. We did not observe any obvious colocalization and conclude that S2 cells are not an ideal system for visualizing in vivo formin-microtubule interactions. To better understand Capu's mechanisms of action during oogenesis, we then systematically characterized several classical capu alleles. While all of these capu mutants exhibit severe developmental defects, they variably affect Capu's actin assembly activity in vitro. Together with our microtubule binding data, this indicates that Capu's physiological role extends beyond its ability to nucleate actin filaments. We therefore performed tandem affinity purification experiments to identify novel Capu binding partners and gain additional insight into Capu's role during oogenesis. We report several candidate binding partners involved in such diverse cellular processes as mitochondrial fission, endocytosis, and nuclear import. Though preliminary, these results suggest that Capu has additional uncharacterized functions in development; further investigation may help us uncover the physiological role of Capu-microtubule binding. Ultimately, our findings expand our understanding of Capu's role in Drosophila oogenesis and provide mechanistic insight into formins as coordinators of the actin and microtubule cytoskeletons.

Subjects/Keywords: Molecular biology; Cellular biology; Biochemistry; actin; cytoskeleton; development; formin; microtubule; oogenesis

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APA (6^th Edition):

Roth-Johnson, E. A. (2014). Microtubule binding by the formin Cappuccino and its implications for Drosophila oogenesis. (Thesis). UCLA. Retrieved from http://www.escholarship.org/uc/item/3g87800k

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Roth-Johnson, Elizabeth Anne. “Microtubule binding by the formin Cappuccino and its implications for Drosophila oogenesis.” 2014. Thesis, UCLA. Accessed January 22, 2021. http://www.escholarship.org/uc/item/3g87800k.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Roth-Johnson, Elizabeth Anne. “Microtubule binding by the formin Cappuccino and its implications for Drosophila oogenesis.” 2014. Web. 22 Jan 2021.

Vancouver:

Roth-Johnson EA. Microtubule binding by the formin Cappuccino and its implications for Drosophila oogenesis. [Internet] [Thesis]. UCLA; 2014. [cited 2021 Jan 22]. Available from: http://www.escholarship.org/uc/item/3g87800k.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Roth-Johnson EA. Microtubule binding by the formin Cappuccino and its implications for Drosophila oogenesis. [Thesis]. UCLA; 2014. Available from: http://www.escholarship.org/uc/item/3g87800k

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Alberta

20. Pisio, Amanda C. The function of nonmuscle myosins during oogenesis in Caenorhabditis elegans.

Degree: MS, Department of Biological Sciences, 2013, University of Alberta

URL: https://era.library.ualberta.ca/files/gb19f655k

► Oogenesis in C. elegans requires the activity of type II nonmuscle myosins, complexes containing two heavy and four light chains that bind and move actin.… (more)

Subjects/Keywords: MLC-4; Caenorhabditis elegans; NMY-1; nonmuscle myosins; Oogenesis; NMY-2

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APA (6^th Edition):

Pisio, A. C. (2013). The function of nonmuscle myosins during oogenesis in Caenorhabditis elegans. (Masters Thesis). University of Alberta. Retrieved from https://era.library.ualberta.ca/files/gb19f655k

Chicago Manual of Style (16^th Edition):

Pisio, Amanda C. “The function of nonmuscle myosins during oogenesis in Caenorhabditis elegans.” 2013. Masters Thesis, University of Alberta. Accessed January 22, 2021. https://era.library.ualberta.ca/files/gb19f655k.

MLA Handbook (7^th Edition):

Pisio, Amanda C. “The function of nonmuscle myosins during oogenesis in Caenorhabditis elegans.” 2013. Web. 22 Jan 2021.

Vancouver:

Pisio AC. The function of nonmuscle myosins during oogenesis in Caenorhabditis elegans. [Internet] [Masters thesis]. University of Alberta; 2013. [cited 2021 Jan 22]. Available from: https://era.library.ualberta.ca/files/gb19f655k.

Council of Science Editors:

Pisio AC. The function of nonmuscle myosins during oogenesis in Caenorhabditis elegans. [Masters Thesis]. University of Alberta; 2013. Available from: https://era.library.ualberta.ca/files/gb19f655k

21. 정, 성수. Makorin 1, Drosophila orthologue of human mkrn3 is required for oogenesis.

Degree: 2017, Ajou University

URL: http://repository.ajou.ac.kr/handle/201003/16441 ; http://dcoll.ajou.ac.kr:9080/dcollection/jsp/common/DcLoOrgPer.jsp?sItemId=000000024824

►

Precocious puberty is puberty occurring at an unusually early age. According to the recent study, a family with precocious puberty is associated with a mutation… (more)

▼

Precocious puberty is puberty occurring at an unusually early age. According to the recent study, a family with precocious puberty is associated with a mutation in the makorin 3(mkrn3) gene. To determine whether a change in mkrn3 causes similar effects on Drosophila as it does in mammals and to test in vivo and find the mechanism, we chose mkrn1 in drosophila because it is a orthologue of mkrn3. We made mkrn1 deletion mutant flies by imprecise excision of p-element. To determine the effect of this mutant line on growth and maturation. We checked various aspect of fly. mkrn1exS flies has longer pupation time and pupa length than control flies. Also, we found that mkrn1exS is much more heavier and consumed food than male and control female fly. Altogether, these results reveal that mkrn1exS affects drosophila growth and maturation. When we crossed the mutant fly we were able to find that female homozygous mutant drosophila lose their fertility. In the case of ovaries from control flies, vitellogenesis occurs, resulting in the formation of yolk and eggs. In the case of ovaries from mkrn1exS, however, vitellogenesis does not occur, resulting in infertility. To find out why vitellogenesis does not occur, we checked the InR/TOR signaling pathway. We have confirmed that the mkrn1exS has high activity of InR/TOR signaling pathway. However, abnormal InR/TOR signaling pathway does not seem to be the reason why vitellogenesis does not occur because it is still working. Also, we checked the Notch signaling pathway. As a result, it was found that mkrn1exS has an abnormal Notch signaling pathway. In addition,.we found that the number of follicle cells expressing cyclin B is higher in mkrn1exS. From these results, we thought that vitellogenesis does not occur because of the abnormality of Notch signaling pathway, which is essential for controlling the cell cycle of the follicle cell. This result is supported by the fact that the cell cycle regulator, Cyclin B, is abnormal. However, it is not yet clear whether this is the direct cause of infertility. Taken together these results, mkrn1 is required for drosophila oogenesis. However, exact mechanism is unknown yet.

ABSTRACT i TABLE OF CONTENTS iii LIST OF FIGURES v LIST OF TABLES vi I. INTRODUCTION 1 II. MATERIALS AND METHODS 4 A. Excision fly strain 4 B. Ovary dissection 5 C. Genomic DNA extraction 5 D. RTPCR 6 E. TUNEL staining 6 F. Immunoblot 7 G. Immunohistochemistry 8 H. Pupation time measurement 9 I. Feeding assay 10 III. RESULT 11 A. Deletion mutation of MKRN1 gene 11 B. mRNA and protein expression pattern in MKRN1 gene deletion mutant 15 C. mkrn1exS affects Drosophila growth and maturation 17 D. mkrn1exS lose their fertility 20 E. Ovaries of mkrn1exS mutant halt development 22 F. mkrn1exS has abnormal InR/TOR signaling pathway 27 G. mkrn1exS has abnormal Notch signaling pathway 31 H. A large number of follicle cells express Cyc B in mkrn1exS 35 I. Abnormal expression of cytoskeletal protein in mkrn1exS 37 DISCUSSION 39 …

Advisors/Committee Members: 대학원 의생명과학과, 201424575, 정, 성수.

Subjects/Keywords: Oogenesis; Vitellogenesis; Drosophila; Puberty; Growth; Maturation; 난형성; 난황형성; 초파리

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APA (6^th Edition):

정, �. (2017). Makorin 1, Drosophila orthologue of human mkrn3 is required for oogenesis. (Thesis). Ajou University. Retrieved from http://repository.ajou.ac.kr/handle/201003/16441 ; http://dcoll.ajou.ac.kr:9080/dcollection/jsp/common/DcLoOrgPer.jsp?sItemId=000000024824

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

정, 성수. “Makorin 1, Drosophila orthologue of human mkrn3 is required for oogenesis.” 2017. Thesis, Ajou University. Accessed January 22, 2021. http://repository.ajou.ac.kr/handle/201003/16441 ; http://dcoll.ajou.ac.kr:9080/dcollection/jsp/common/DcLoOrgPer.jsp?sItemId=000000024824.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

정, 성수. “Makorin 1, Drosophila orthologue of human mkrn3 is required for oogenesis.” 2017. Web. 22 Jan 2021.

Vancouver:

정 �. Makorin 1, Drosophila orthologue of human mkrn3 is required for oogenesis. [Internet] [Thesis]. Ajou University; 2017. [cited 2021 Jan 22]. Available from: http://repository.ajou.ac.kr/handle/201003/16441 ; http://dcoll.ajou.ac.kr:9080/dcollection/jsp/common/DcLoOrgPer.jsp?sItemId=000000024824.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

정 �. Makorin 1, Drosophila orthologue of human mkrn3 is required for oogenesis. [Thesis]. Ajou University; 2017. Available from: http://repository.ajou.ac.kr/handle/201003/16441 ; http://dcoll.ajou.ac.kr:9080/dcollection/jsp/common/DcLoOrgPer.jsp?sItemId=000000024824

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Toronto

22. Vujadinovic, Stefan. The Okapi (Oka) FERM Domain Protein Regulates Somatic Stem Cell Numbers and Cell Intercalation during Drosophila Oogenesis.

Degree: 2017, University of Toronto

URL: http://hdl.handle.net/1807/97275

►

Okapi (Oka) is a Drosophila melanogaster FERM domain protein that is essential for oogenesis. oka (CG34347) mutants are viable but display defects during oogenesis. Mutant… (more)

Subjects/Keywords: Drosophila melanogaster; FERM Domain Protein; Intercalation; Oogenesis; Proliferation; Stem Cells; 0758

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APA (6^th Edition):

Vujadinovic, S. (2017). The Okapi (Oka) FERM Domain Protein Regulates Somatic Stem Cell Numbers and Cell Intercalation during Drosophila Oogenesis. (Masters Thesis). University of Toronto. Retrieved from http://hdl.handle.net/1807/97275

Chicago Manual of Style (16^th Edition):

Vujadinovic, Stefan. “The Okapi (Oka) FERM Domain Protein Regulates Somatic Stem Cell Numbers and Cell Intercalation during Drosophila Oogenesis.” 2017. Masters Thesis, University of Toronto. Accessed January 22, 2021. http://hdl.handle.net/1807/97275.

MLA Handbook (7^th Edition):

Vujadinovic, Stefan. “The Okapi (Oka) FERM Domain Protein Regulates Somatic Stem Cell Numbers and Cell Intercalation during Drosophila Oogenesis.” 2017. Web. 22 Jan 2021.

Vancouver:

Vujadinovic S. The Okapi (Oka) FERM Domain Protein Regulates Somatic Stem Cell Numbers and Cell Intercalation during Drosophila Oogenesis. [Internet] [Masters thesis]. University of Toronto; 2017. [cited 2021 Jan 22]. Available from: http://hdl.handle.net/1807/97275.

Council of Science Editors:

Vujadinovic S. The Okapi (Oka) FERM Domain Protein Regulates Somatic Stem Cell Numbers and Cell Intercalation during Drosophila Oogenesis. [Masters Thesis]. University of Toronto; 2017. Available from: http://hdl.handle.net/1807/97275

University of Maine

23. Rossin, Ashley. The Effects of Ocean Acidification on the Gametogenesis of the Red Tree Coral, Primnoa pacifica.

Degree: MS, Marine Biology, 2018, University of Maine

URL: https://digitalcommons.library.umaine.edu/etd/2866

► Primnoa pacifica, otherwise known as the Red Tree Coral, is one of the most ecologically important corals in the North Pacific. This species is… (more)

▼ Primnoa pacifica, otherwise known as the Red Tree Coral, is one of the most ecologically important corals in the North Pacific. This species is an ecosystem engineer, providing essential habitat for commercially important fish and invertebrate species. Ocean acidification (OA) threatens corals and all calcifying organisms and is more prevalent in polar and sub-polar regions as the concentration of CO₂ is higher and there is a lowered buffering capacity due to low alkalinity in colder waters. The impact from a chemical shift in the oceans could alter the role of P. pacifica as an ecosystem engineer in predicted ocean conditions. An experiment was conducted to assess the impacts of OA on the gametogenesis of Red Tree Corals from Tracy Arm fjord in Alaska. Primnoa pacifica colonies were cultured for six to nine months in either projected 2100 pH (7.55 pH units) or Ambient pH (7.75 pH units), then were prepared for histology to investigate any changes in gametogenesis in acidified water. Oocyte diameters and fecundities were significantly lower in the 2100 samples. The females from the experiment also had a higher proportion of individuals experiencing reabsorption of vitellogenic oocytes, potentially to preserve the lipids for other ecological processes as a stress response. The highest percentage of oosorption was seen in the 2100 females, as well as the smallest oocytes and the lowest fecundities. There was a “null tank effect” observed in all measurement types, however these only significantly affected the analyses of the exterior measurements and spermatogenesis. When compared to a published 2014 reproductive dataset, all the tank samples were statistically different from previous years and were significantly lower. These results indicate that reproduction in this species may not be possible in the current pH predictions for 2100, or that if spawning could still occur, any spawned oocyte may not be sufficiently equipped to support a larva. This study only investigated one life history stage of these long-lived organisms and more research spanning multiple life history stages needs to be done in their natural habitat as well as in homogeneous laboratory conditions. There is also a need to investigate the probability of these corals spawning under environmental stressors to identify if these important species will be present in the future. Advisors/Committee Members: Rhian Waller, Bob Steneck, Aaron Strong.

Subjects/Keywords: Gulf of Alaska; gametogenesis; Primnoa; histology; oogenesis; ocean acidification; Marine Biology

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APA (6^th Edition):

Rossin, A. (2018). The Effects of Ocean Acidification on the Gametogenesis of the Red Tree Coral, Primnoa pacifica. (Masters Thesis). University of Maine. Retrieved from https://digitalcommons.library.umaine.edu/etd/2866

Chicago Manual of Style (16^th Edition):

Rossin, Ashley. “The Effects of Ocean Acidification on the Gametogenesis of the Red Tree Coral, Primnoa pacifica.” 2018. Masters Thesis, University of Maine. Accessed January 22, 2021. https://digitalcommons.library.umaine.edu/etd/2866.

MLA Handbook (7^th Edition):

Rossin, Ashley. “The Effects of Ocean Acidification on the Gametogenesis of the Red Tree Coral, Primnoa pacifica.” 2018. Web. 22 Jan 2021.

Vancouver:

Rossin A. The Effects of Ocean Acidification on the Gametogenesis of the Red Tree Coral, Primnoa pacifica. [Internet] [Masters thesis]. University of Maine; 2018. [cited 2021 Jan 22]. Available from: https://digitalcommons.library.umaine.edu/etd/2866.

Council of Science Editors:

Rossin A. The Effects of Ocean Acidification on the Gametogenesis of the Red Tree Coral, Primnoa pacifica. [Masters Thesis]. University of Maine; 2018. Available from: https://digitalcommons.library.umaine.edu/etd/2866

University of Otago

24. Ranay Ahavan, Sobhan. Lipid physiology in beluga (Huso huso L.) - the relationship between developmental stages, 11-ketotestosterone levels and ovarian lipid uptake .

Degree: University of Otago

URL: http://hdl.handle.net/10523/9404

► In egg-laying fish, survival of the embryonic and initial larval stages depends on accumulation of substantial reserves of yolk and lipid within the egg during… (more)

▼ In egg-laying fish, survival of the embryonic and initial larval stages depends on accumulation of substantial reserves of yolk and lipid within the egg during oocyte growth. In sturgeon, a non-teleost basal ray-finned fish, the relationship between lipid physiology and oogenesis has not been investigated. Gonadal development is arrested in the early stages of maturation. However, androgens have been implicated in regulation of early follicular growth in several vertebrate classes. Considering such gaps in the literature, this thesis, therefore, aimed to elucidate key physiological and molecular changes associated with lipid biology during oocyte development in beluga, or great sturgeon. Moreover, the effects of 11-ketotestosterone treatment on ovarian growth and mRNA levels of putative lipidation-related genes were investigated in pre-vitellogenic (PV) ovaries of sturgeon. To do so, as a first attempt, the developmental stages of oocytes from captive beluga were classified through an analysis of general appearance of intact oocytes after non-lethal biopsy techniques which was followed by histological observations (Chapter 2). The different stages of oocyte growth were standardized based on morphological criteria. As such, ovaries of captive beluga were classified as being in the perinucleolar (PN), oil droplet (OD), primary yolk (PY), secondary yolk (SY) or tertiary yolk (TY) stage. To focus on sturgeon lipid physiology, an investigation was conducted to determine changes in lipid transport, concentration of sex steroids, and expression of genes that have been implicated in ovarian lipid uptake during the reproductive cycle (Chapter, 3). The lipoprotein profile changes were determined using fast protein liquid chromatography (FPLC). Also, the triglycerides (TGs), and cholesterol changes in the FPLC extracted fractions of lipoprotein profiles were determined. Plasma levels of sex steroid hormones (17β-estradiol (E2) and 11-ketotestosterone (11-KT)) and lipid (TGs, cholesterol and phospholipid) were elevated during gonadal development. Using gel filtration, low levels of TGs and cholesterol were observed in different lipoprotein classes across different stages of oogenesis, but no differences were found. Albumin, vitellogenin (Vtg) AB2b, immunoglobulin light chain precursor and immunoglobulin heavy chain were detected as components of different lipoprotein fractions by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-MS). The expression of housekeeping genes was significantly affected by gonadal development. Also, a high abundance of 5S ribosomal RNA transcripts was detected in early stages of gonadal development. In this study the geometric means of ribosomal RNA (5S, 18S and 28S rRNA) was used to normalize the transcript abundance of putative lipidation-related genes. The transcript abundances of lipoprotein lipase (lpl), and apolipoprotein e (apoe) and lipoprotein receptors, i.e., very-low density lipoprotein receptors (vldlr), and low-density lipoprotein receptor-related… Advisors/Committee Members: Lokman, Peter Mark (advisor).

Subjects/Keywords: Sturgeon; Oogenesis; Pre-vitellogenesis

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APA (6^th Edition):

Ranay Ahavan, S. (n.d.). Lipid physiology in beluga (Huso huso L.) - the relationship between developmental stages, 11-ketotestosterone levels and ovarian lipid uptake . (Doctoral Dissertation). University of Otago. Retrieved from http://hdl.handle.net/10523/9404

Note: this citation may be lacking information needed for this citation format:
No year of publication.

Chicago Manual of Style (16^th Edition):

Ranay Ahavan, Sobhan. “Lipid physiology in beluga (Huso huso L.) - the relationship between developmental stages, 11-ketotestosterone levels and ovarian lipid uptake .” Doctoral Dissertation, University of Otago. Accessed January 22, 2021. http://hdl.handle.net/10523/9404.

Note: this citation may be lacking information needed for this citation format:
No year of publication.

MLA Handbook (7^th Edition):

Ranay Ahavan, Sobhan. “Lipid physiology in beluga (Huso huso L.) - the relationship between developmental stages, 11-ketotestosterone levels and ovarian lipid uptake .” Web. 22 Jan 2021.

Note: this citation may be lacking information needed for this citation format:
No year of publication.

Vancouver:

Ranay Ahavan S. Lipid physiology in beluga (Huso huso L.) - the relationship between developmental stages, 11-ketotestosterone levels and ovarian lipid uptake . [Internet] [Doctoral dissertation]. University of Otago; [cited 2021 Jan 22]. Available from: http://hdl.handle.net/10523/9404.

Note: this citation may be lacking information needed for this citation format:
No year of publication.

Council of Science Editors:

Ranay Ahavan S. Lipid physiology in beluga (Huso huso L.) - the relationship between developmental stages, 11-ketotestosterone levels and ovarian lipid uptake . [Doctoral Dissertation]. University of Otago; Available from: http://hdl.handle.net/10523/9404

Note: this citation may be lacking information needed for this citation format:
No year of publication.

Northeastern University

25. MacDonald, Julie. Exploring Solutions To Ovarian Aging Through Systemic To Subcellular Approaches.

Degree: PhD, Department of Biology, 2019, Northeastern University

URL: http://hdl.handle.net/2047/D20316320

► Ovarian failure is an unavoidable diagnosis for all women, and yet the exact mechanism remains unknown, particularly regarding premature ovarian failure - in which case… (more)

Subjects/Keywords: Aging; Fertility; Mitochondria; Oogenesis; Ovary; Stem Cell; Cellular biology

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APA (6^th Edition):

MacDonald, J. (2019). Exploring Solutions To Ovarian Aging Through Systemic To Subcellular Approaches. (Doctoral Dissertation). Northeastern University. Retrieved from http://hdl.handle.net/2047/D20316320

Chicago Manual of Style (16^th Edition):

MacDonald, Julie. “Exploring Solutions To Ovarian Aging Through Systemic To Subcellular Approaches.” 2019. Doctoral Dissertation, Northeastern University. Accessed January 22, 2021. http://hdl.handle.net/2047/D20316320.

MLA Handbook (7^th Edition):

MacDonald, Julie. “Exploring Solutions To Ovarian Aging Through Systemic To Subcellular Approaches.” 2019. Web. 22 Jan 2021.

Vancouver:

MacDonald J. Exploring Solutions To Ovarian Aging Through Systemic To Subcellular Approaches. [Internet] [Doctoral dissertation]. Northeastern University; 2019. [cited 2021 Jan 22]. Available from: http://hdl.handle.net/2047/D20316320.

Council of Science Editors:

MacDonald J. Exploring Solutions To Ovarian Aging Through Systemic To Subcellular Approaches. [Doctoral Dissertation]. Northeastern University; 2019. Available from: http://hdl.handle.net/2047/D20316320

University of Iowa

26. Spracklen, Andrew James. Prostaglandin signaling temporally regulates actin cytoskeletal remodeling during Drosophila oogenesis.

Degree: PhD, Molecular and Cellular Biology, 2014, University of Iowa

URL: https://ir.uiowa.edu/etd/1399

► Prostaglandins (PGs) are small, lipid signaling molecules produced downstream of cyclooxygenase (COX) enzymes. PG signaling regulates many processes including pain, inflammation, fertility, cardiovascular function… (more)

▼ Prostaglandins (PGs) are small, lipid signaling molecules produced downstream of cyclooxygenase (COX) enzymes. PG signaling regulates many processes including pain, inflammation, fertility, cardiovascular function and disease, and cancer. One mechanism by which PG signaling exerts its function is by regulating the dynamics of the actin cytoskeleton; however, the exact mechanisms remain largely undefined. Drosophila oogenesis provides an ideal system to determine how PG signaling regulates the actin cytoskeleton. Drosophila follicles, or eggs, pass through 14 well- characterized, morphologically defined stages of development. Each developing follicle is comprised of 16 interconnected germline-derived cells (15 nurse cells and 1 oocyte) that are surrounded by a layer of somatically derived epithelial cells. During Stage 10B (S10B), the nurse cells form a cage-like network of parallel actin filament bundles that extend from the nurse cell membranes inward, toward the nurse cell nuclei. During Stage 11 (S11), the nurse cells rapidly transfer their cytoplasmic contents into the oocyte in an actomysoin-dependent contraction termed nurse cell dumping. Previous work uncovered that the Drosophila COX-like enzyme, Peroxinectin-like (Pxt), and thus PG signaling, is required to promote both actin filament bundle formation during S10B and subsequent nurse cell dumping. This finding established Drosophila oogenesis as a genetically tractable model in which to elucidate the conserved mechanisms underlying PG- dependent actin remodeling. The research presented in this dissertation focused on identifying actin-binding proteins that are regulated by PG signaling during Drosophila oogenesis. To identify these downstream effectors, we performed a dominant modifier screen to uncover factors that could suppress or enhance the ability of COX inhibitors to block nurse cell dumping in vitro. This screen revealed a number of actin-binding proteins that enhance the dumping defects caused by COX-inhibition, including the actin bundling protein, Fascin (Drosophila Singed, Sn); the actin filament elongation factor, Enabled (Ena); and the actin filament capper, Capping protein (Drosophila Capping protein alpha, Cpa, and beta, Cpb). Through a collaborative effort between Christopher Groen and myself, Fascin was shown to mediate PG-dependent cortical actin integrity and actin bundle formation during Drosophila ooogenesis. Ena and Capping protein regulate actin filament elongation through opposing actions: Ena promotes their elongation, while Capping protein binds to, or caps, the growing end of actin filaments to prevent their further elongation. However, genetic reduction of either Ena or Capping protein enhance the nurse cell dumping defects caused by COX inhibition. These findings suggest that Ena activity must be balanced to promote proper actin remodeling during S10B. Ena localization to the growing ends of actin filament bundles is reduced in pxt… Advisors/Committee Members: Tootle, Tina L. (supervisor).

Subjects/Keywords: actin; cytoskeleton; Drosophila; Enabled; oogenesis; prostaglandins; Cell Biology

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APA (6^th Edition):

Spracklen, A. J. (2014). Prostaglandin signaling temporally regulates actin cytoskeletal remodeling during Drosophila oogenesis. (Doctoral Dissertation). University of Iowa. Retrieved from https://ir.uiowa.edu/etd/1399

Chicago Manual of Style (16^th Edition):

Spracklen, Andrew James. “Prostaglandin signaling temporally regulates actin cytoskeletal remodeling during Drosophila oogenesis.” 2014. Doctoral Dissertation, University of Iowa. Accessed January 22, 2021. https://ir.uiowa.edu/etd/1399.

MLA Handbook (7^th Edition):

Spracklen, Andrew James. “Prostaglandin signaling temporally regulates actin cytoskeletal remodeling during Drosophila oogenesis.” 2014. Web. 22 Jan 2021.

Vancouver:

Spracklen AJ. Prostaglandin signaling temporally regulates actin cytoskeletal remodeling during Drosophila oogenesis. [Internet] [Doctoral dissertation]. University of Iowa; 2014. [cited 2021 Jan 22]. Available from: https://ir.uiowa.edu/etd/1399.

Council of Science Editors:

Spracklen AJ. Prostaglandin signaling temporally regulates actin cytoskeletal remodeling during Drosophila oogenesis. [Doctoral Dissertation]. University of Iowa; 2014. Available from: https://ir.uiowa.edu/etd/1399

University of Iowa

27. Soshnev, Alexey Aleksandrovich. New transcriptional roles for the classic Drosophila insulator protein Suppressor of Hairy-wing.

Degree: PhD, Molecular and Cellular Biology, 2012, University of Iowa

URL: https://ir.uiowa.edu/etd/1505

► The Drosophila Suppressor of Hairy-wing [Su(Hw)] protein is a multi-zinc finger DNA binding factor required for the gypsy insulator function. At the gypsy element,… (more)

▼ The Drosophila Suppressor of Hairy-wing [Su(Hw)] protein is a multi-zinc finger DNA binding factor required for the gypsy insulator function. At the gypsy element, Su(Hw) recruits partners Centrosomal Protein of 190 kD (CP190) and Modifier of mdg4 67.2 kD isoform (Mod67.2), which facilitate the enhancer blocking and barrier functions of the insulator. Our genome-wide studies have identified thousands of endogenous non-gypsy Su(Hw) binding sites (SBSs) in Drosophila genome, constitutively occupied throughout development. Yet, only a third of SBSs associate with CP190 and Mod67.2, suggesting that the endogenous function of Su(Hw) may not necessarily involve formation of a gypsy-like chromatin insulator. To understand the function of endogenous SBSs, we investigated the requirement for Su(Hw) during female germline development. To this end, we performed genome-wide transcriptional analyses in su(Hw) mutant ovaries coupled with the genome-wide definition of ovary SBSs. We identified 49 direct targets of Su(Hw) regulation in the ovary, with 80% of these genes showing increased RNA accumulation when Su(Hw) is lost. Derepressed Su(Hw) targets are normally highly expressed in central nervous system, suggesting that Su(Hw) has a critical role in silencing neural genes in the non-neural tissues. We find that a single upregulated target gene is largely responsible for the female sterility of the su(Hw) mutants. This gene encodes an elav family factor RNA binding protein 9 (Rbp9), and Su(Hw)-mediated repression of Rbp9 is required at a specific stage of germline development. Decreasing the levels of Rbp9 restores female fertility of su(Hw) null mutants. Further, we demonstrate that Su(Hw) is required for transcriptional activation of pointed, gene involved in eggshell patterning during late embryogenesis. Importantly, both CP190 and Mod67.2 are dispensable for Rbp9 regulation, indicating that mechanism of Rbp9 repression is independent of the insulator function of Su(Hw). Our studies extend the known transcriptional activities of Su(Hw), indicating that it can function as an insulator, activator and repressor, the latter function being essential for oogenesis. These findings highlight that insulator proteins are versatile transcriptional regulators, suggesting that tissue specific contributions to transcription result from direct regulation of individual genes. Advisors/Committee Members: Geyer, Pamela Kent, 1956- (supervisor).

Subjects/Keywords: Chromatin; Drosophila; Insulator; Oogenesis; Su(Hw); Cell Biology

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APA (6^th Edition):

Soshnev, A. A. (2012). New transcriptional roles for the classic Drosophila insulator protein Suppressor of Hairy-wing. (Doctoral Dissertation). University of Iowa. Retrieved from https://ir.uiowa.edu/etd/1505

Chicago Manual of Style (16^th Edition):

Soshnev, Alexey Aleksandrovich. “New transcriptional roles for the classic Drosophila insulator protein Suppressor of Hairy-wing.” 2012. Doctoral Dissertation, University of Iowa. Accessed January 22, 2021. https://ir.uiowa.edu/etd/1505.

MLA Handbook (7^th Edition):

Soshnev, Alexey Aleksandrovich. “New transcriptional roles for the classic Drosophila insulator protein Suppressor of Hairy-wing.” 2012. Web. 22 Jan 2021.

Vancouver:

Soshnev AA. New transcriptional roles for the classic Drosophila insulator protein Suppressor of Hairy-wing. [Internet] [Doctoral dissertation]. University of Iowa; 2012. [cited 2021 Jan 22]. Available from: https://ir.uiowa.edu/etd/1505.

Council of Science Editors:

Soshnev AA. New transcriptional roles for the classic Drosophila insulator protein Suppressor of Hairy-wing. [Doctoral Dissertation]. University of Iowa; 2012. Available from: https://ir.uiowa.edu/etd/1505

Boston University

28. Kleinsorge, Sarah Elizabeth. The role of cell polarity during cell fate specification and programmed cell death in the drosophila ovary.

Degree: PhD, Genetics & Genomics, 2015, Boston University

URL: http://hdl.handle.net/2144/13988

► As an organism develops, multiple cellular processes need to occur in order to specify and organize tissue. One essential process is the establishment of cell… (more)

▼ As an organism develops, multiple cellular processes need to occur in order to specify and organize tissue. One essential process is the establishment of cell polarity, which drives cell fate specification and stem cell differentiation. Another key process is programmed cell death, which is important for tissue remodeling and clearing damaged or diseased cells from the body. A loss in cell polarity can lead to defects in tissue organization and carcinogenesis. Defects in programmed cell death can lead to autoimmune diseases and cancer. However, hyperactive programmed cell death can lead to neurodegeneration. The Drosophila ovary, which is composed of germline and somatic cells, is an excellent model to study both cell polarity and cell death. In the germ cells, oocyte fate is specified and maintained through the asymmetric localization of cell cycle and cell polarity RNAs, proteins, and organelles, such as mitochondria, to and within the oocyte. Additionally the somatic follicle cells, which surround the germ cells, require a specific apical-basal polarity to function. During oogenesis, programmed cell death can be induced within the ovary to prevent oogenesis from maturing under low nutrient, high stress or crowded conditions. When this occurs, the germline is cleared from the ovary by a process known as engulfment. Somatic follicle cells surrounding the germline synchronously enlarge and engulf the corpses of the dying germline cells. It is unknown what triggers the enlargement of the follicle cells. Previous research has shown that the apical side of a follicle cell is heavily marked by cell polarity proteins, to specify the apical side away from the lateral and basal sides. Since many important genes regulating both cell polarity and engulfment are conserved between Drosophila and other eukaryotes, we can study the establishment and maintenance of cell polarity and its role during engulfment to obtain a better understanding of these processes in mammals and their relevance to diseases. This dissertation investigates the role of cell polarity in both the specification of oocyte cell fate, and the organization and enlargement of the follicle cells during engulfment in the ovary.

Subjects/Keywords: Genetics; Engulfment; Mitochondria; Oogenesis; Cell death; Cell polarity

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APA (6^th Edition):

Kleinsorge, S. E. (2015). The role of cell polarity during cell fate specification and programmed cell death in the drosophila ovary. (Doctoral Dissertation). Boston University. Retrieved from http://hdl.handle.net/2144/13988

Chicago Manual of Style (16^th Edition):

Kleinsorge, Sarah Elizabeth. “The role of cell polarity during cell fate specification and programmed cell death in the drosophila ovary.” 2015. Doctoral Dissertation, Boston University. Accessed January 22, 2021. http://hdl.handle.net/2144/13988.

MLA Handbook (7^th Edition):

Kleinsorge, Sarah Elizabeth. “The role of cell polarity during cell fate specification and programmed cell death in the drosophila ovary.” 2015. Web. 22 Jan 2021.

Vancouver:

Kleinsorge SE. The role of cell polarity during cell fate specification and programmed cell death in the drosophila ovary. [Internet] [Doctoral dissertation]. Boston University; 2015. [cited 2021 Jan 22]. Available from: http://hdl.handle.net/2144/13988.

Council of Science Editors:

Kleinsorge SE. The role of cell polarity during cell fate specification and programmed cell death in the drosophila ovary. [Doctoral Dissertation]. Boston University; 2015. Available from: http://hdl.handle.net/2144/13988

Boston University

29. Candelas, Pelagia Graciela. Follicle cell actin dynamics and calcium bursts during nurse cell death in Drosophila melanogaster.

Degree: 2019, Boston University

URL: http://hdl.handle.net/2144/37106

► Cell death is a key component in development and for the continued renewal of tissues. Phagoptosis is a process in which phagocytes directly lead to… (more)

▼ Cell death is a key component in development and for the continued renewal of tissues. Phagoptosis is a process in which phagocytes directly lead to the death of other cells. This process of cell death is significantly less characterized when compared to other mechanisms of cell death, such as apoptosis. In the Drosophila ovary, phagoptosis appears to play a key role in the developmental process of oogenesis. Recent studies have shown that genes associated with phagocytosis are required for the programmed death of nurse cells in the Drosophila ovary. Ovaries are made up of 15 nurse cells, a single oocyte, and a layer of follicle cells bordering them. During the process of egg chamber development, all of the nurse cells undergo programmed cell death. During late oogenesis, each nurse cell is surrounded by a group of follicle cells referred to as stretch follicle cells. These stretch follicle cells have recently been implicated as a main promoter of nurse cell phagoptosis. However, an exact mechanism to explain how these stretch follicle cells induce nurse cell death is not fully characterized. To achieve a more detailed understanding of this mechanism, we are examining the function of the cytoskeleton in this process via live imaging. We hypothesize that the follicle cell cytoskeleton plays a significant role in nurse death due to the importance of actin during phagocytosis. Further, we intend to use these live imaging studies to investigate the role of calcium before, during, and after clearance of the nurse cells. Previous studies have shown that calcium bursts within the cell are associated with the initiation of phagocytosis in macrophages, as well as other phagocytic cell types. Studies in this thesis were done by utilizing live imaging and have shown dynamic changes in follicle cell actin before and during the death of nurse cells. These confocal microscopy real time videos have revealed that follicle cell actin polymerizes towards the nurse cell immediately before acidification. Following acidification of the nurse cells, the follicle cell actin changes direction, moving towards the phagocytic follicle cell. Additionally, through live imaging we have observed calcium bursts in the follicle cells immediately before nurse cell death. Overall, this work has provided a more detailed understanding of nurse cell death. Advisors/Committee Members: McCall, Kim (advisor).

Subjects/Keywords: Biology; Actin; Calcium; Cell death; Drosophila melanogaster; Follicle cell; Oogenesis

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Candelas, P. G. (2019). Follicle cell actin dynamics and calcium bursts during nurse cell death in Drosophila melanogaster. (Thesis). Boston University. Retrieved from http://hdl.handle.net/2144/37106

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Candelas, Pelagia Graciela. “Follicle cell actin dynamics and calcium bursts during nurse cell death in Drosophila melanogaster.” 2019. Thesis, Boston University. Accessed January 22, 2021. http://hdl.handle.net/2144/37106.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Candelas, Pelagia Graciela. “Follicle cell actin dynamics and calcium bursts during nurse cell death in Drosophila melanogaster.” 2019. Web. 22 Jan 2021.

Vancouver:

Candelas PG. Follicle cell actin dynamics and calcium bursts during nurse cell death in Drosophila melanogaster. [Internet] [Thesis]. Boston University; 2019. [cited 2021 Jan 22]. Available from: http://hdl.handle.net/2144/37106.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Candelas PG. Follicle cell actin dynamics and calcium bursts during nurse cell death in Drosophila melanogaster. [Thesis]. Boston University; 2019. Available from: http://hdl.handle.net/2144/37106

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Adelaide

30. Irving-Rodgers, Helen. Extracellular matrix and the development and atresia of bovine ovarian follicles.

Degree: 2007, University of Adelaide

URL: http://hdl.handle.net/2440/59618

► The studies submitted for this thesis encompass two broad areas of interest. The first is the role of extracellular matrix during folliculogenesis, including ovulation and… (more)

Subjects/Keywords: extracellular matrix; Oogenesis; Corpus luteum

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Irving-Rodgers, H. (2007). Extracellular matrix and the development and atresia of bovine ovarian follicles. (Thesis). University of Adelaide. Retrieved from http://hdl.handle.net/2440/59618

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Irving-Rodgers, Helen. “Extracellular matrix and the development and atresia of bovine ovarian follicles.” 2007. Thesis, University of Adelaide. Accessed January 22, 2021. http://hdl.handle.net/2440/59618.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Irving-Rodgers, Helen. “Extracellular matrix and the development and atresia of bovine ovarian follicles.” 2007. Web. 22 Jan 2021.

Vancouver:

Irving-Rodgers H. Extracellular matrix and the development and atresia of bovine ovarian follicles. [Internet] [Thesis]. University of Adelaide; 2007. [cited 2021 Jan 22]. Available from: http://hdl.handle.net/2440/59618.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Irving-Rodgers H. Extracellular matrix and the development and atresia of bovine ovarian follicles. [Thesis]. University of Adelaide; 2007. Available from: http://hdl.handle.net/2440/59618

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

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Open Access Theses and Dissertations