Advanced search options

Advanced Search Options 🞨

Browse by author name (“Author name starts with…”).

Find ETDs with:

in
/  
in
/  
in
/  
in

Written in Published in Earliest date Latest date

Sorted by

Results per page:

Sorted by: relevance · author · university · dateNew search

You searched for subject:(oocyte degeneration). Showing records 1 – 2 of 2 total matches.

Search Limiters

Last 2 Years | English Only

No search limiters apply to these results.

▼ Search Limiters


Texas A&M University

1. Taiyeb-Ridha, Ahmed 1979-. Evaluation of Contraceptive Properties of Cilostazol (A Phosphodiesterase 3A Inhibitor) in Mice.

Degree: PhD, Biomedical Sciences, 2012, Texas A&M University

The pharmacological development of non-steroidal contraceptives has yet to be achieved. Arresting oocyte maturation without blocking ovulation has been evaluated using different inhibitors of the phosphodiesterase 3A (PDE3A). Unfortunately, PDE3A is also expressed in the heart and blood vessels, and inhibition of PDE3A in oocytes can produce cardiovascular side effects. We reviewed the literature on available PDE3 inhibitors and selected cilostazol (CLZ), which is an FDA approved therapeutic. CLZ has the ability to decrease cellular adenosine uptake and consequently antagonizes side effects of PDE3A inhibition in vital organs. CLZ inhibited oocyte meiotic maturation in vitro. CLZ has more degenerative impact on arrested oocytes than matured oocytes, indicating that prolonged meiotic arrest of oocytes is harmful. Administration of CLZ any time from 9h before the ovulatory stimulus to 4h after the stimulus resulted in ovulation of immature oocytes. Controlling CLZ dose, time of CLZ administration, and time of oocyte collection resulted in ovulation of oocytes at different meiotic stages. Oral administrations of CLZ in naturally cycling mice were also observed to block pregnancy whereas remating of those previously treated females resulted in normal offspring and litter sizes. Therefore, CLZ does not only have a wide margin of contraception but also is reversible. Ovulated immature oocytes were observed to have higher rates of advanced chromatin configuration and cortical granule distribution, normal spindle and chromosomal organization, maturation, and in vitro fertilization (IVF) than ovarian immature oocytes. Ovulated metaphase I oocytes that were matured in vitro or in vivo had higher IVF rates than ovulated mature oocytes. Ovulated germinal vesicle (GV) oocytes that were in vitro matured also showed higher IVF rates but when in vivo matured, they had lower IVF rates than ovulated mature oocytes because of the high degeneration and low fertilization rates associated with in vivo maturation of GV oocytes. In summary, CLZ merits further evaluation as a non-steroidal contraceptive and is capable of producing oocytes of various meiotic stages with advanced developmental features. Advisors/Committee Members: Kraemer, Duane C (advisor), Fajt, Virginia (advisor), Les Dees, W (committee member), Sayes, Christie M (committee member).

Subjects/Keywords: in vitro fertilization.; oocyte degeneration; oocyte maturation synchronization; oocyte maturation; phosphodiesterase three inhibitor; Cilostazol

Record DetailsSimilar RecordsGoogle PlusoneFacebookTwitterCiteULikeMendeleyreddit

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6th Edition):

Taiyeb-Ridha, A. 1. (2012). Evaluation of Contraceptive Properties of Cilostazol (A Phosphodiesterase 3A Inhibitor) in Mice. (Doctoral Dissertation). Texas A&M University. Retrieved from http://hdl.handle.net/1969.1/148278

Chicago Manual of Style (16th Edition):

Taiyeb-Ridha, Ahmed 1979-. “Evaluation of Contraceptive Properties of Cilostazol (A Phosphodiesterase 3A Inhibitor) in Mice.” 2012. Doctoral Dissertation, Texas A&M University. Accessed October 30, 2020. http://hdl.handle.net/1969.1/148278.

MLA Handbook (7th Edition):

Taiyeb-Ridha, Ahmed 1979-. “Evaluation of Contraceptive Properties of Cilostazol (A Phosphodiesterase 3A Inhibitor) in Mice.” 2012. Web. 30 Oct 2020.

Vancouver:

Taiyeb-Ridha A1. Evaluation of Contraceptive Properties of Cilostazol (A Phosphodiesterase 3A Inhibitor) in Mice. [Internet] [Doctoral dissertation]. Texas A&M University; 2012. [cited 2020 Oct 30]. Available from: http://hdl.handle.net/1969.1/148278.

Council of Science Editors:

Taiyeb-Ridha A1. Evaluation of Contraceptive Properties of Cilostazol (A Phosphodiesterase 3A Inhibitor) in Mice. [Doctoral Dissertation]. Texas A&M University; 2012. Available from: http://hdl.handle.net/1969.1/148278


University of Alberta

2. Ullah, A.K.M. Shahid. Determining The Critical Weight Of The Rocky Mountain Wood Ticks Dermacentor andersoni Stiles (Acari: Ixodidae).

Degree: MS, Department of Biological Sciences, 2012, University of Alberta

The feeding cycle of female ixodid ticks is divided into preparatory, slow and rapid feeding phases. Female ticks, virgin or mated, reach a ‘critical weight’ (CW; 10-13X the unfed weight) at the transition between the slow and rapid feeding phases, which is characterized by several physiological and behavioural changes. To date, the CW has been determined for Amblyomma hebraeum only. This study established that, in both mated and virgin Dermacentor Andersoni, salivary gland degeneration and ovarian development are triggered when the tick exceeds 10-13X the unfed weight. However, mated ticks undergo these changes 4 days earlier than virgin ticks. Unlike A. hebraeum, virgin D. andersoni regularly fed well beyond their CW, and occasionally fed up to 100X their unfed weight.

Subjects/Keywords: Dermacentor andersoni, ixodid tick, Rocky mountain wood tick, critical weight, salivary gland degeneration, ovary weight, oocyte length, ovary vitellin content, reattachment

Record DetailsSimilar RecordsGoogle PlusoneFacebookTwitterCiteULikeMendeleyreddit

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6th Edition):

Ullah, A. K. M. S. (2012). Determining The Critical Weight Of The Rocky Mountain Wood Ticks Dermacentor andersoni Stiles (Acari: Ixodidae). (Masters Thesis). University of Alberta. Retrieved from https://era.library.ualberta.ca/files/qf85nc26t

Chicago Manual of Style (16th Edition):

Ullah, A K M Shahid. “Determining The Critical Weight Of The Rocky Mountain Wood Ticks Dermacentor andersoni Stiles (Acari: Ixodidae).” 2012. Masters Thesis, University of Alberta. Accessed October 30, 2020. https://era.library.ualberta.ca/files/qf85nc26t.

MLA Handbook (7th Edition):

Ullah, A K M Shahid. “Determining The Critical Weight Of The Rocky Mountain Wood Ticks Dermacentor andersoni Stiles (Acari: Ixodidae).” 2012. Web. 30 Oct 2020.

Vancouver:

Ullah AKMS. Determining The Critical Weight Of The Rocky Mountain Wood Ticks Dermacentor andersoni Stiles (Acari: Ixodidae). [Internet] [Masters thesis]. University of Alberta; 2012. [cited 2020 Oct 30]. Available from: https://era.library.ualberta.ca/files/qf85nc26t.

Council of Science Editors:

Ullah AKMS. Determining The Critical Weight Of The Rocky Mountain Wood Ticks Dermacentor andersoni Stiles (Acari: Ixodidae). [Masters Thesis]. University of Alberta; 2012. Available from: https://era.library.ualberta.ca/files/qf85nc26t

.