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1.
Saurabh, Suman.
Nature of Inter-biomolecular interaction and its consequences : protein, DNA and their Complexes : Nature de l'interaction inter-biomoléculaire : protéine, ADN et leurs complexes.
Degree: Docteur es, Physique, 2017, Université François-Rabelais de Tours
URL: http://www.theses.fr/2017TOUR4052 
► Le monde biologique est rempli de mystères. La compréhension de nombreux processus biologiques extrêmement complexes est grandement améliorée par la combinaison d’approches empruntées à différentes…
(more)
▼ Le monde biologique est rempli de mystères. La compréhension de nombreux processus biologiques extrêmement complexes est grandement améliorée par la combinaison d’approches empruntées à différentes disciplines telles que la chimie et plus récemment la physique. La physique utilise des outils expérimentaux tels que les pinces optiques et les microscopies optique et électronique pour explorer les mécanismes à l’échelle microscopique se déroulant dans la cellule. La connaissance de la nature des interactions entre biomolécules et la possibilité de traduire ces interactions en équations ont permis à la physique de construire des modèles simples mais contenant les ingrédients suffisants à la description d’un mécanisme spécifique. La simulation numérique de tels modèles permet d’améliorer notre compréhension de la relation entre les mécanismes pertinents à l’échelle moléculaire et les observations expérimentales de phénomènes biologiques.
The biological world is full of mysteries. The understanding of many extremely complex biological processes is greatly improved by the combination of approaches borrowed from different disciplines such as chemistry and more recently physics. Physics uses experimental tools such as optical tweezers and optical and electron microscopes to explore the microscopic mechanisms taking place in the cell. Knowledge of the nature of the interactions between biomolecules and the possibility of translating these interactions into equations allowed physics to construct models that are simple, but contain the ingredients sufficient to describe a specific mechanism. The numerical simulation of such models improves our understanding of the relationship between relevant molecular-scale mechanisms and experimental observations of biological phenomena. The structural organization of biomolecular complexes is a process that involves various scales of length and time.
Advisors/Committee Members: Lansac, Yves (thesis director).
Subjects/Keywords: Cristal liquide; Nucléosomes; Complexes biomoléculaires; DNA; Nucleosome Core Particle; Histone; Potential of Mean Force; Liquid crystal; HIV; Dendrimer; Stacking
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APA (6th Edition):
Saurabh, S. (2017). Nature of Inter-biomolecular interaction and its consequences : protein, DNA and their Complexes : Nature de l'interaction inter-biomoléculaire : protéine, ADN et leurs complexes. (Doctoral Dissertation). Université François-Rabelais de Tours. Retrieved from http://www.theses.fr/2017TOUR4052
Chicago Manual of Style (16th Edition):
Saurabh, Suman. “Nature of Inter-biomolecular interaction and its consequences : protein, DNA and their Complexes : Nature de l'interaction inter-biomoléculaire : protéine, ADN et leurs complexes.” 2017. Doctoral Dissertation, Université François-Rabelais de Tours. Accessed March 09, 2021.
http://www.theses.fr/2017TOUR4052.
MLA Handbook (7th Edition):
Saurabh, Suman. “Nature of Inter-biomolecular interaction and its consequences : protein, DNA and their Complexes : Nature de l'interaction inter-biomoléculaire : protéine, ADN et leurs complexes.” 2017. Web. 09 Mar 2021.
Vancouver:
Saurabh S. Nature of Inter-biomolecular interaction and its consequences : protein, DNA and their Complexes : Nature de l'interaction inter-biomoléculaire : protéine, ADN et leurs complexes. [Internet] [Doctoral dissertation]. Université François-Rabelais de Tours; 2017. [cited 2021 Mar 09].
Available from: http://www.theses.fr/2017TOUR4052.
Council of Science Editors:
Saurabh S. Nature of Inter-biomolecular interaction and its consequences : protein, DNA and their Complexes : Nature de l'interaction inter-biomoléculaire : protéine, ADN et leurs complexes. [Doctoral Dissertation]. Université François-Rabelais de Tours; 2017. Available from: http://www.theses.fr/2017TOUR4052
University of Illinois – Chicago
2.
Shim, Yoonjung.
Coexpression and Reconstitution of Multiprotein Complexes
in Eukaryotic Nucleotide Excision Repair.
Degree: 2018, University of Illinois – Chicago
URL: http://hdl.handle.net/10027/23060
► Genetic transactions such as DNA replication, transcription, recombination, and repair are often initiated and controlled by dynamic interactions among intricate multiprotein complexes and chromatin DNA.…
(more)
▼ Genetic transactions such as DNA replication, transcription, recombination, and repair are often initiated and controlled by dynamic interactions among intricate multiprotein complexes and chromatin DNA. While it is crucial to study the structural, biochemical and biophysical mechanisms of the interactions involving these molecules, such studies are often challenged by the difficulties in reconstituting large protein and protein-DNA complexes in sufficient purity and quantity.
In this thesis, I focused on developing methods to prepare key such complexes that have seminal roles in several genetic transactions in eukaryotes, including transcription and nucleotide excision repair (NER). First, I describe the co-expression of histone octamer complex and the reconstitution of the
nucleosome core particles (NCPs). NCPs are basic organization and packaging units of DNA in all eukaryotic cells, consisting of ~147 bp DNA wrapped around histone octamers. Nucleosomes are folded into compact, highly organized chromatin structures, which form the working platform for various DNA mechanisms including transcription and NER. Second, I discuss the co-expression and purification of the 5-subunit
core of the general transcription factor II H complex (TFIIH) complex using MultiBac expression system. TFIIH is essential for trancription initiaton as well as NER DNA damage processing. Lastly, I describe preliminary studies on the interactions between TFIIH and Rad4-Rad23 complex, a key initial damage recognition factor in NER. The TFIIH-Rad4 interaction constitutes a key initiation step in NER that removes various environmentally induced DNA lesions and protect genomic integrity in cells.
Advisors/Committee Members: Min, Jung-Hyun (advisor), Cho, Wonhwa (committee member), Fung, Leslie Wo-Mei (committee member), Miller, Lawrence (committee member), Kay, Brian (committee member), Min, Jung-Hyun (chair).
Subjects/Keywords: Multiprotein complex; Eukaryotic nucleotide excision repair; Histone octamers; Nucleosome core particle (NCP); General transcription factor II H (TFIIH)
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APA (6th Edition):
Shim, Y. (2018). Coexpression and Reconstitution of Multiprotein Complexes
in Eukaryotic Nucleotide Excision Repair. (Thesis). University of Illinois – Chicago. Retrieved from http://hdl.handle.net/10027/23060
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Shim, Yoonjung. “Coexpression and Reconstitution of Multiprotein Complexes
in Eukaryotic Nucleotide Excision Repair.” 2018. Thesis, University of Illinois – Chicago. Accessed March 09, 2021.
http://hdl.handle.net/10027/23060.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Shim, Yoonjung. “Coexpression and Reconstitution of Multiprotein Complexes
in Eukaryotic Nucleotide Excision Repair.” 2018. Web. 09 Mar 2021.
Vancouver:
Shim Y. Coexpression and Reconstitution of Multiprotein Complexes
in Eukaryotic Nucleotide Excision Repair. [Internet] [Thesis]. University of Illinois – Chicago; 2018. [cited 2021 Mar 09].
Available from: http://hdl.handle.net/10027/23060.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Shim Y. Coexpression and Reconstitution of Multiprotein Complexes
in Eukaryotic Nucleotide Excision Repair. [Thesis]. University of Illinois – Chicago; 2018. Available from: http://hdl.handle.net/10027/23060
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Virginia Tech
3.
Ruscio, Jory Zmuda.
Molecular Modeling: Elucidation of Structure/Function Relationships of Proteins and DNA at the Atomic Resolution.
Degree: PhD, Genetics, Bioinformatics, and Computational Biology, 2007, Virginia Tech
URL: http://hdl.handle.net/10919/27219
► While experiments provide valuable information about biological molecules, current technology cannot yet monitor atomic fluctuations at relevant time scales. Theoretical computational simulations are able to…
(more)
▼ While experiments provide valuable information about biological molecules, current technology cannot yet monitor atomic fluctuations at relevant time scales. Theoretical computational simulations are able to model the appropriate interactions at atomic resolution. Computational techniques have become widely used for identifying interactions in biological systems. Such methods have proven quite accurate in their ability to reproduce experimental data and also in screening and predicting pertinent activities. Molecular modeling employs theoretical and computational techniques to elucidate biologically relevant information from macromolecular structures. Three biological systems, the
nucleosome core particle, myoglobin and glycosyl hydrolase family 1 beta-glucosidases will be examined with molecular modeling methods. Results of our analyses provide information about DNA flexibility and packaging, internal migration of ligands in a small protein, and substrate specificity of an enzyme system.
Advisors/Committee Members: Murali, T. M. (committee member), Sandu, Adrian (committee member), Esen, Asim (committee member), Bevan, David R. (committeecochair), Onufriev, Alexey V. (committeecochair).
Subjects/Keywords: nucleosome core particle; myoglobin; beta-glucosidases; molecular dynamics
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APA (6th Edition):
Ruscio, J. Z. (2007). Molecular Modeling: Elucidation of Structure/Function Relationships of Proteins and DNA at the Atomic Resolution. (Doctoral Dissertation). Virginia Tech. Retrieved from http://hdl.handle.net/10919/27219
Chicago Manual of Style (16th Edition):
Ruscio, Jory Zmuda. “Molecular Modeling: Elucidation of Structure/Function Relationships of Proteins and DNA at the Atomic Resolution.” 2007. Doctoral Dissertation, Virginia Tech. Accessed March 09, 2021.
http://hdl.handle.net/10919/27219.
MLA Handbook (7th Edition):
Ruscio, Jory Zmuda. “Molecular Modeling: Elucidation of Structure/Function Relationships of Proteins and DNA at the Atomic Resolution.” 2007. Web. 09 Mar 2021.
Vancouver:
Ruscio JZ. Molecular Modeling: Elucidation of Structure/Function Relationships of Proteins and DNA at the Atomic Resolution. [Internet] [Doctoral dissertation]. Virginia Tech; 2007. [cited 2021 Mar 09].
Available from: http://hdl.handle.net/10919/27219.
Council of Science Editors:
Ruscio JZ. Molecular Modeling: Elucidation of Structure/Function Relationships of Proteins and DNA at the Atomic Resolution. [Doctoral Dissertation]. Virginia Tech; 2007. Available from: http://hdl.handle.net/10919/27219
The Ohio State University
4.
Conroy, Daniel William.
Structural Studies of Biomolecules by Dynamic Nuclear
Polarization Solid-State NMR Spectroscopy.
Degree: PhD, Chemistry, 2019, The Ohio State University
URL: http://rave.ohiolink.edu/etdc/view?acc_num=osu1555428362333615
► Solid-state NMR (SSNMR) spectroscopy is an incredibly powerful tool for studying the structure and dynamics of biomolecules and large macromolecular complexes. SSNMR has no inherent…
(more)
▼ Solid-state NMR (SSNMR) spectroscopy is an incredibly
powerful tool for studying the structure and dynamics of
biomolecules and large macromolecular complexes. SSNMR has no
inherent size restriction and is useful in studying non-crystalline
protein such as membrane-bound protein or amyloid fibrils,
DNA-protein complexes, and large protein assemblies such as the
HIV-capsid. Meanwhile, techniques such as solution-state NMR and
x-ray crystallography are strictly limited by molecular size and
sample condition, and thus cannot study large biomolecules or
insoluble protein aggregates, respectively. The first protein at
the center of this study is the human prion protein (PrP) and its
formation into an insoluble amyloid fibril. The formation of this
fibril leads to a deposition of an insoluble plaque on the central
nervous system which leads to the development of the prion protein
disease, known as Gerstmann-Straussler-Scheinker (GSS) disease. The
second system-of-interest is the
nucleosome core particle (NCP)
which is a DNA-protein complex and is the building-block of
chromatin and chromosomes. An individual NCP is composed of dsDNA
wrapped around an octamer of histone protein, mimicking the natural
phenomena of DNA storage and compaction in eukaryotic cells. SSNMR
is uniquely qualified in studying these biological systems in depth
to characterize the amyloid fibril propagation and disease-causing
mechanism of prion protein, and to explore nucleic acid
base-pairing behavior at DNA-protein interfaces and the important
interactions therein.Dynamic nuclear polarization (DNP) is a
hyperpolarization technique for NMR spectroscopy which dramatically
increases the overall sensitivity of these experiments. In
DNP-SSNMR, hyperpolarization is achieved by applying microwave
irradiation to free electrons, often in the form of
stable-radicals, within a static magnetic field and inducing a
polarization transfer to neighboring nuclear spins, especially
spin-½ protons. These elevated levels of magnetization in protons
can be passed on to other NMR-active nuclei, in particular
carbon-13 (13C) and nitrogen-15 (15N), using standard SSNMR
experimental protocols. This process can generate NMR signal
enhancements ranging from 20 to 200 and may be generally applied to
small molecules, solid-phase materials and biological samples. DNP
offers significant time savings when performing complex SSNMR
experiments and opens the door for studying dilute species, weak
interactions, or minor populations which are typically below the
threshold of standard NMR spectroscopy.The primary goal of this
dissertation is to develop strategies to study the structures of
prion protein amyloid fibrils and dsDNA by expanding on current
DNP-SSNMR methodologies, as well as introducing a novel class of
biradicals. First, new polarizing agents, peptide-based biradicals
are synthesized, purified, characterized and are successfully
employed to study small molecules, crystalline protein, and amyloid
protein by DNP-SSNMR. These dinitroxides include TOAC-TOAC (TT),
TOAC-Ser-TOAC…
Advisors/Committee Members: Jaroniec, Christopher (Advisor).
Subjects/Keywords: Chemistry; Solid-State NMR Spectroscopy; Dynamic Nuclear Polarization; Dinitroxide; Biradical; Polarizing Agent; Solid-Phase Peptide Synthesis; TOAC; DNA; Hoogsteen Base-Pairing; Nucleosome Core Particle; Amyloid Fibril; Prion Protein
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APA ·
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CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Conroy, D. W. (2019). Structural Studies of Biomolecules by Dynamic Nuclear
Polarization Solid-State NMR Spectroscopy. (Doctoral Dissertation). The Ohio State University. Retrieved from http://rave.ohiolink.edu/etdc/view?acc_num=osu1555428362333615
Chicago Manual of Style (16th Edition):
Conroy, Daniel William. “Structural Studies of Biomolecules by Dynamic Nuclear
Polarization Solid-State NMR Spectroscopy.” 2019. Doctoral Dissertation, The Ohio State University. Accessed March 09, 2021.
http://rave.ohiolink.edu/etdc/view?acc_num=osu1555428362333615.
MLA Handbook (7th Edition):
Conroy, Daniel William. “Structural Studies of Biomolecules by Dynamic Nuclear
Polarization Solid-State NMR Spectroscopy.” 2019. Web. 09 Mar 2021.
Vancouver:
Conroy DW. Structural Studies of Biomolecules by Dynamic Nuclear
Polarization Solid-State NMR Spectroscopy. [Internet] [Doctoral dissertation]. The Ohio State University; 2019. [cited 2021 Mar 09].
Available from: http://rave.ohiolink.edu/etdc/view?acc_num=osu1555428362333615.
Council of Science Editors:
Conroy DW. Structural Studies of Biomolecules by Dynamic Nuclear
Polarization Solid-State NMR Spectroscopy. [Doctoral Dissertation]. The Ohio State University; 2019. Available from: http://rave.ohiolink.edu/etdc/view?acc_num=osu1555428362333615
5.
Weng, Liwei.
Investigating the reactivity of an abasic lesion within nucleosomes and probing the interactions between histone tails and nucleosomal DNA in nucleosome core particles.
Degree: 2015, Johns Hopkins University
URL: http://jhir.library.jhu.edu/handle/1774.2/37902
► DNA lesions are the source of the cytotoxicity of various antitumor reagents and γ-radiolysis. Studying the reactivity of these lesions and their effects in biological…
(more)
▼ DNA lesions are the source of the cytotoxicity of various antitumor reagents and γ-radiolysis. Studying the reactivity of these lesions and their effects in biological processes may provide the chemical basis for anticancer treatment and shed light on the rational design of novel drugs. The oxidized abasic lesion 5’-(2-phosphoryl-1,4-dioxobutane) (DOB) is produced concomitantly with a strand break via C5'-hydrogen atom abstraction by a variety of DNA-damaging agents. Herein, we report that the reactivity of DOB is distinct in nucleosomes from that in free DNA. DOB undergoes cleavage at a significantly higher rate in NCPs compared to free DNA, exhibiting a half-life of 8.5-16.8 min depending upon the sites in which DOB is generated. The rate constant of DOB decomposition is faster than its expected rate of repair in cells. DOB decomposition results in a pyrrolone modification of the histone tail. Furthermore, DOB decomposition within the linker DNA that connects two
adjacent nucleosomes is also accelerated, albeit to a lesser extent than that within NCPs. The pyrrolone modification was observed in the histone H3 as a result of the interactions of the H3 tail interacting with the lesion in the linker region. The unnatural modification resulting from reaction with the DNA lesion may have profound biological consequences. The interactions between histone tails and nucleosomal DNA play significant roles in chromatin assembly and the regulation of gene expression. Recent studies including the one presented above revealed that residues on the N-terminal tails of histone proteins are intensively involved in catalyzing the decomposition of DNA lesions at specific sites within NCPs. Despite their importance, such interactions are not well defined due to the mobility of histone tails and the transient nature of the interactions. To gain insight into such interactions, we developed a new DNA-protein cross-linking (DPC) method by taking advantage of the
reaction between a modified electrophilic nucleotide (2) and the nucleophilic residues in histone tails. Phenyl selenide 1 rapidly produces 2 upon mild oxidation, which reacts with nucleophilic amino acid side chains. The facile reaction and high DPC yields produced from 2 enabled both product and kinetic analysis, which ranked the contributions by individual and/or groups of amino acids (His18 > Lys16 > Lys20 ~ Lys8,12 > Lys5) that react with 2 at position 89 within the NCP, a hot spot for DNA damage. In addition, photolysis of 1 produced ICLs in free DNA and NCPs. The reexamination of photochemistry of 1 revealed that a carbocation intermediate (19) is responsible for the DNA ICL formation.
Advisors/Committee Members: Greenberg, Marc M (advisor).
Subjects/Keywords: DNA abasic lesion;
DOB;
nucleosome;
DNA-protein cross-links;
nucleosome core particle;
histone tails;
histone tail-DNA interactions
…produces ICL in DNA
20
Figure 11. Structure of the nucleosome core particle
26
Figure 12. The… …histone tails on AP reactivity.
42
Figure 27. C4-AP cleavage in a nucleosome core particle… …44
Figure 28. Strand cleavage at L in a nucleosome core particle.
45
Figure 29… …mass spectrometry
MWCO
molecular weight cut-off
NCP
nucleosome core particle
NCS… …nucleosome core particles
1.
Introduction
144
2.
Background
148
2.1 DNA-protein interactions…
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APA ·
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APA (6th Edition):
Weng, L. (2015). Investigating the reactivity of an abasic lesion within nucleosomes and probing the interactions between histone tails and nucleosomal DNA in nucleosome core particles. (Thesis). Johns Hopkins University. Retrieved from http://jhir.library.jhu.edu/handle/1774.2/37902
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Weng, Liwei. “Investigating the reactivity of an abasic lesion within nucleosomes and probing the interactions between histone tails and nucleosomal DNA in nucleosome core particles.” 2015. Thesis, Johns Hopkins University. Accessed March 09, 2021.
http://jhir.library.jhu.edu/handle/1774.2/37902.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Weng, Liwei. “Investigating the reactivity of an abasic lesion within nucleosomes and probing the interactions between histone tails and nucleosomal DNA in nucleosome core particles.” 2015. Web. 09 Mar 2021.
Vancouver:
Weng L. Investigating the reactivity of an abasic lesion within nucleosomes and probing the interactions between histone tails and nucleosomal DNA in nucleosome core particles. [Internet] [Thesis]. Johns Hopkins University; 2015. [cited 2021 Mar 09].
Available from: http://jhir.library.jhu.edu/handle/1774.2/37902.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Weng L. Investigating the reactivity of an abasic lesion within nucleosomes and probing the interactions between histone tails and nucleosomal DNA in nucleosome core particles. [Thesis]. Johns Hopkins University; 2015. Available from: http://jhir.library.jhu.edu/handle/1774.2/37902
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Université Paris-Sud – Paris XI
6.
Lemercier, Nicolas.
CEMOVIS, développements méthodologiques et étude ultrastructurale de la cellule HT29 : De la cellule aux nucléosomes : CEMOVIS methodological developments and structural study of HT 29 cell : from cell to nucleosoms.
Degree: Docteur es, Biologie cellulaire et moléculaire, 2012, Université Paris-Sud – Paris XI
URL: http://www.theses.fr/2012PA114812
► Nous avons utilisé la méthode de CEMOVIS (Cryo-Electron Microscopy Of Vitreous Sections) pour étudier l’ultrastructure des cellules HT29 (lignée cancéreuse colique humaine) et plus particulièrement…
(more)
▼ Nous avons utilisé la méthode de CEMOVIS (Cryo-Electron Microscopy Of Vitreous Sections) pour étudier l’ultrastructure des cellules HT29 (lignée cancéreuse colique humaine) et plus particulièrement l’organisation de la chromatine au sein du noyau. Pour améliorer la méthode, nous avons développé un micromanipulateur qui facilite la collecte des coupes et leur transfert sur la grille. Nous avons également cherché à préparer de nouveaux films métalliques (en remplacement du carbone) permettant une meilleure adhésion des coupes sur le support Au vu des premiers tests réalisés, les films de TiO2 que nous avons fabriqués au laboratoire et caractérisés par microscopie électronique (HR, spectroscopie et cartographie EELS) semblent offrir des perspectives intéressantes que nous attribuons à leur propriétés de conducteur électrique à basse température (ce qui reste à démontrer). Les organites cellulaires (noyaux, réseaux de filaments du cytosquelette, systèmes multilamellaires) ont été identifiés in situ. Les conditions d’imagerie choisies nous ont permis d’obtenir une résolution permettant d’identifier les deux feuillets des bicouches membranaires. Dans le noyau, nous avons observé des motifs striés, distants de 2.7 à 3.5 nm que nous attribuons à la molécule d’ADN enroulée autour du cœur d’histones. Comparées aux images de phases denses de nucléosomes, ces images suggèrent que les nucléosomes (jamais identifiés in situ jusqu’à présent) présentent un ordre très local au sein de la chromatine, que nous discutons à la lumières des modèles polymériques actuels.
The ultrastructure of HT29 cells (human epithelial adenocarcinoma cell line) was studied by CEMOVIS (Cryo-Electron Microscopy of Vitreous Sections) with a special emphasis on chromatin organization in the cell nucleus. We proposed methodological improvements for this technique:- We first developed a grid holding micromanipulator to facilitate both cryosections collect and deposition on carbon-coated TEM grids.- We also developed new metallic thin films (to replace carbon-base supports) to enhance the adhesion of cryosections on their support. The TiO2 thin films that we produced and analysed by electron microscopy (high resolution imaging, EELS and chemical mapping) seem to be an interesting alternative to carbon films for the deposition of cryosections. Their adhesive properties could be due to Titanium high electric conductance at low temperature (although this relation has not been clearly demonstrated yet).In HT 29 cells, we indentified cell organites (nucleus; cytoskeleton filament bundles, multilamellar bodies) in situ. Selected imaging conditions provide for a high enough resolution to visualise the two membrane leaflets. In the cell nucleus, we observed striated patterns separated from 2.7 to 3.5 nm that we assume to be DNA molecule turns wrapped around the histone protein core. Compared with the dense phases formed in vitro by nucleosome core particle in solution, our images suggest that nucleosomes are locally ordered in chromatin. This observation is…
Advisors/Committee Members: Livolant, Françoise (thesis director), Leforestier, Amélie (thesis director).
Subjects/Keywords: CEMOVIS; Cryo-microscopie électronique; Cryosections; Cryo-ultramicrotome; Particules Coeur de Nucléosome; Cellules HT29; Phase hexagonale; Bicouches; Film de carbone; Alliage titane-silicium; Micromanipulateur; CEMOVIS; Cryo-electron microscopy; Cryosections; Cryo-ultramicrotomy; Nucleosome Core Particle (NCP); Chromatin; Nucleus; HT29; Désoxyribonucleic acid (DNA); Hexagonal phase; Bilayers; Carbon films; Titanium-Silicon alloy; EELS; Electric conductivity; Micromanipulator
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APA ·
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MLA ·
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CSE |
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Manager
APA (6th Edition):
Lemercier, N. (2012). CEMOVIS, développements méthodologiques et étude ultrastructurale de la cellule HT29 : De la cellule aux nucléosomes : CEMOVIS methodological developments and structural study of HT 29 cell : from cell to nucleosoms. (Doctoral Dissertation). Université Paris-Sud – Paris XI. Retrieved from http://www.theses.fr/2012PA114812
Chicago Manual of Style (16th Edition):
Lemercier, Nicolas. “CEMOVIS, développements méthodologiques et étude ultrastructurale de la cellule HT29 : De la cellule aux nucléosomes : CEMOVIS methodological developments and structural study of HT 29 cell : from cell to nucleosoms.” 2012. Doctoral Dissertation, Université Paris-Sud – Paris XI. Accessed March 09, 2021.
http://www.theses.fr/2012PA114812.
MLA Handbook (7th Edition):
Lemercier, Nicolas. “CEMOVIS, développements méthodologiques et étude ultrastructurale de la cellule HT29 : De la cellule aux nucléosomes : CEMOVIS methodological developments and structural study of HT 29 cell : from cell to nucleosoms.” 2012. Web. 09 Mar 2021.
Vancouver:
Lemercier N. CEMOVIS, développements méthodologiques et étude ultrastructurale de la cellule HT29 : De la cellule aux nucléosomes : CEMOVIS methodological developments and structural study of HT 29 cell : from cell to nucleosoms. [Internet] [Doctoral dissertation]. Université Paris-Sud – Paris XI; 2012. [cited 2021 Mar 09].
Available from: http://www.theses.fr/2012PA114812.
Council of Science Editors:
Lemercier N. CEMOVIS, développements méthodologiques et étude ultrastructurale de la cellule HT29 : De la cellule aux nucléosomes : CEMOVIS methodological developments and structural study of HT 29 cell : from cell to nucleosoms. [Doctoral Dissertation]. Université Paris-Sud – Paris XI; 2012. Available from: http://www.theses.fr/2012PA114812
. 
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