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You searched for subject:(muscadines). Showing records 1 – 2 of 2 total matches.

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1. Dansby, Montreka. Evaluation of the Antioxidant and Biological Properties of Muscadine Grape Seed Extracts.

Degree: PhD, Food Science, 2007, North Carolina State University

The objectives of this research were i) to optimize extraction of phenolic compounds from muscadine grape seeds using food grade solvents, ii) to characterize and quantify the phenolic compounds present in Carlos muscadine grape seed extracts, iii) to assess the oxidative stability of the grape seed extract, iv) to evaluate the antimicrobial activity of the optimal muscadine grape seed extract, and v) to evaluate the effect of muscadine grape seed extract on MCF-10A breast cells. Carlos muscadine grape seeds were manually removed from the skin and pulp, then freeze-dried or oven-dried. Phenolic compounds were extracted from muscadine grape seeds using a Dionex Accelerated Solvent Extractor 200 following a two factor composite design. The two factors were solvent and temperature. Ethanol in combination with water and ethyl acetate in combination with water were evaluated in this research. Solvent percentages ranged from 35% to 95% and temperature ranged from 75C to 150C. Optimal extraction conditions were determined using the Folin-Ciocalteu and oxygen radical absorbance capacity assays. Identification and quantification of the phenolic compounds were determined by high performance liquid chromatography with mass spectroscopy. The optimal extraction conditions for the highest recovery of phenolic compounds consisted of the freeze-dried seeds extracted with 65% ethanol and 35% water at 150°C, with total phenolic and oxygen radical absorbance capacity values of 5.87 mg GAE⁄g and 60.32 M-TE⁄g of seeds, respectively. The four major phenolic compounds quantified in the muscadine grape seed extract were epicatechin, catechin, gallic acid and ellagic acid. Oxidative stability index of the muscadine grape seed extract resulted in an induction period of 4.6 hours. In the antimicrobial study, both muscadine grape seed extract and ellagic acid inhibited the growth of E. coli 0157:H7, Listeria monocytogenes, and Salmonella typhimurium at 104 cfu⁄ml. These research findings show that muscadine grape seed extracts contain phytochemicals with antioxidant and antimicrobial properties that can be used in applications including food ingredients, food supplements and other nutraceutical applications. Advisors/Committee Members: Dr. Leon Boyd, Committee Chair (advisor).

Subjects/Keywords: antioxidants; anti-microbial; anti-carcinogenic; grape seed; polyphenols; muscadines

…6 Biological Properties of Muscadines … 7 French Paradox… …the southeastern United States. Muscadines differ substantially from other grape species in… …caused by bacterium Xylella fastidiosa. Native muscadines have a natural resistance to… …muscadine grape contribute to cultivation and handling durability of muscadines, however it… …muscadines. According to Pastrana-Bonilla et al. (2003), the total phenolics in… 

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6th Edition):

Dansby, M. (2007). Evaluation of the Antioxidant and Biological Properties of Muscadine Grape Seed Extracts. (Doctoral Dissertation). North Carolina State University. Retrieved from http://www.lib.ncsu.edu/resolver/1840.16/5328

Chicago Manual of Style (16th Edition):

Dansby, Montreka. “Evaluation of the Antioxidant and Biological Properties of Muscadine Grape Seed Extracts.” 2007. Doctoral Dissertation, North Carolina State University. Accessed December 02, 2020. http://www.lib.ncsu.edu/resolver/1840.16/5328.

MLA Handbook (7th Edition):

Dansby, Montreka. “Evaluation of the Antioxidant and Biological Properties of Muscadine Grape Seed Extracts.” 2007. Web. 02 Dec 2020.

Vancouver:

Dansby M. Evaluation of the Antioxidant and Biological Properties of Muscadine Grape Seed Extracts. [Internet] [Doctoral dissertation]. North Carolina State University; 2007. [cited 2020 Dec 02]. Available from: http://www.lib.ncsu.edu/resolver/1840.16/5328.

Council of Science Editors:

Dansby M. Evaluation of the Antioxidant and Biological Properties of Muscadine Grape Seed Extracts. [Doctoral Dissertation]. North Carolina State University; 2007. Available from: http://www.lib.ncsu.edu/resolver/1840.16/5328


University of Georgia

2. Snipes, Lauren. Inhibition of human aldose reductase by high phenolic and high anti-oxidative extracts of muscadine grape fractions.

Degree: 2014, University of Georgia

Muscadines are berries of Vitis rotundifolia, a grape native to SE USA. Muscadine skins and seeds are sold as nutraceutical products because of their high antioxidant activity, anti-inflammatory and anti-cancer phytochemical profiles. Hyperglycemia leads to both induction and activation of aldose reductase (AR), the initial rate-limiting enzyme of the polyol pathway (conversion of glucose to sorbitol to fructose). Activation of the polyol pathway contributes to Advanced Glycation Endproduct (AGE) formation in organs with AR activity including lens of the eye, kidney, heart, nerves and liver. This pathway is implicated in diabetic cataract formation and neuropathies. Because the pharmacognosy of muscadine skins revealed many compounds with anti-AR activity, we tested the ability of muscadine skin and seed extracts to inhibit human recombinant AR (HRAR) in vitro. The results indicated that extracts of muscadine skins and seeds inhibited AR activity at low concentrations. Nutraceutical products made from muscadine skins and seeds may prove useful in inhibiting AGE formation via inhibition of the polyol pathway.

Subjects/Keywords: Muscadines are berries of Vitis rotundifolia; a grape native to SE USA. Muscadine skins and seeds are sold as nutraceutical products because of their high antioxidant activity; anti-inflammatory and anti-cancer phytochemical profiles. Hyperglycemia leads

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6th Edition):

Snipes, L. (2014). Inhibition of human aldose reductase by high phenolic and high anti-oxidative extracts of muscadine grape fractions. (Thesis). University of Georgia. Retrieved from http://hdl.handle.net/10724/23527

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16th Edition):

Snipes, Lauren. “Inhibition of human aldose reductase by high phenolic and high anti-oxidative extracts of muscadine grape fractions.” 2014. Thesis, University of Georgia. Accessed December 02, 2020. http://hdl.handle.net/10724/23527.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7th Edition):

Snipes, Lauren. “Inhibition of human aldose reductase by high phenolic and high anti-oxidative extracts of muscadine grape fractions.” 2014. Web. 02 Dec 2020.

Vancouver:

Snipes L. Inhibition of human aldose reductase by high phenolic and high anti-oxidative extracts of muscadine grape fractions. [Internet] [Thesis]. University of Georgia; 2014. [cited 2020 Dec 02]. Available from: http://hdl.handle.net/10724/23527.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Snipes L. Inhibition of human aldose reductase by high phenolic and high anti-oxidative extracts of muscadine grape fractions. [Thesis]. University of Georgia; 2014. Available from: http://hdl.handle.net/10724/23527

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

.