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NSYSU

1. Liu, Yu-Ting. The Surface Recognition on the VLPs of Dragon Grouper Nervous Necrosis Virus by its Antibodies.

Degree: Master, Marine Biotechnology and Resources, 2011, NSYSU

Grouper in Taiwan is of high value, but nervous necrosis virus infection causes 100% mortality. Our laboratory had developed a good expression system to produce virus-like particles that induced immune functions. In this study, cells producing monoclonal antibody against the virus-like particles were used to induce BALB/c mice production of high titer ascites. The ascite from the H1 cells generated 6000-fold of antibodies higher than cell culture, using enzyme-linked immune-sorbent assay, although relatively less than the previous monoclonal cells of mX, 8000-fold. In the electron microscopy, the ascite antibody bound to various mutants of virus-like particles. Using the polymerase chain reaction to amplify fragments of IgG cDNAs, we will clone and express such cDNAs to efficiently produce the desired monoclonal antibodies. Advisors/Committee Members: Shu-Fen Chiou (chair), Chan-Shing Lin (committee member), Wen Zhi-Hong (chair), Chi-Hsin Hsu (chair), Tian-Lu Cheng (chair).

Subjects/Keywords: grouper nervous necrosis virus; monoclone antibody; virus-like particles; hybridoma; ascites

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6th Edition):

Liu, Y. (2011). The Surface Recognition on the VLPs of Dragon Grouper Nervous Necrosis Virus by its Antibodies. (Thesis). NSYSU. Retrieved from http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0909111-114035

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16th Edition):

Liu, Yu-Ting. “The Surface Recognition on the VLPs of Dragon Grouper Nervous Necrosis Virus by its Antibodies.” 2011. Thesis, NSYSU. Accessed May 08, 2021. http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0909111-114035.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7th Edition):

Liu, Yu-Ting. “The Surface Recognition on the VLPs of Dragon Grouper Nervous Necrosis Virus by its Antibodies.” 2011. Web. 08 May 2021.

Vancouver:

Liu Y. The Surface Recognition on the VLPs of Dragon Grouper Nervous Necrosis Virus by its Antibodies. [Internet] [Thesis]. NSYSU; 2011. [cited 2021 May 08]. Available from: http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0909111-114035.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Liu Y. The Surface Recognition on the VLPs of Dragon Grouper Nervous Necrosis Virus by its Antibodies. [Thesis]. NSYSU; 2011. Available from: http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0909111-114035

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

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