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You searched for subject:(modeled intrinsic clearance). One record found.

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1. Morgan, Alex D. Quantitative Estimation of P-glycorptein-Mediated Drug Transport by Mechanistically Modeled Intrinsic Clearance.

Degree: 2015, University of Western Ontario

P-glycoprotein (P-gp/ABCB1) is an important efflux drug transporter affecting the disposition of 50% of marketed drugs. Cell monolayer permeability assays are the gold standard for assessing P-gp-drug interactions in vitro, but inter-laboratory assay differences produce heterogeneous results. We compared the validity and sensitivity of traditional assay metrics of efflux transport (unidirectional apparent permeability and efflux ratio) with a modeled clearance metric, CLP-gp and hypothesized that CLP-gp would be superior. Cell monolayers heterologously transfected with ABCB1, and 1,25(OH)2D3-modulated ABCB1 in cells served as experimental models. P-gp expression was quantified by western blot and bidirectional [3H]-digoxin transcellular flux was measured. Linear regression analyses were performed for P-gp expression versus each P-gp activity metric. The validity and sensitivity of modeled clearance was comparable to traditional metrics within a cell type, but was superior across different cell types. In conclusion, CLP-gp offers a physiologically-relevant and universally acceptable metric for efflux transport activity.

Subjects/Keywords: P-glycoprotein; P-gp; modeled intrinsic clearance; apparent permeability; efflux ratio; monolayer permeability assay; Pharmacology

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6th Edition):

Morgan, A. D. (2015). Quantitative Estimation of P-glycorptein-Mediated Drug Transport by Mechanistically Modeled Intrinsic Clearance. (Thesis). University of Western Ontario. Retrieved from https://ir.lib.uwo.ca/etd/2961

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16th Edition):

Morgan, Alex D. “Quantitative Estimation of P-glycorptein-Mediated Drug Transport by Mechanistically Modeled Intrinsic Clearance.” 2015. Thesis, University of Western Ontario. Accessed June 15, 2019. https://ir.lib.uwo.ca/etd/2961.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7th Edition):

Morgan, Alex D. “Quantitative Estimation of P-glycorptein-Mediated Drug Transport by Mechanistically Modeled Intrinsic Clearance.” 2015. Web. 15 Jun 2019.

Vancouver:

Morgan AD. Quantitative Estimation of P-glycorptein-Mediated Drug Transport by Mechanistically Modeled Intrinsic Clearance. [Internet] [Thesis]. University of Western Ontario; 2015. [cited 2019 Jun 15]. Available from: https://ir.lib.uwo.ca/etd/2961.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Morgan AD. Quantitative Estimation of P-glycorptein-Mediated Drug Transport by Mechanistically Modeled Intrinsic Clearance. [Thesis]. University of Western Ontario; 2015. Available from: https://ir.lib.uwo.ca/etd/2961

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

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