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You searched for subject:(microRNA biogenesis). Showing records 1 – 3 of 3 total matches.

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UCLA

1. Ward, Taylor Katherine. A global library screen of human primary microRNA processing utilizing a ratiometric fluorescence assay.

Degree: Biochemistry, Molecular and Structural Biology, 2016, UCLA

MicroRNAs (miRNAs) are small, noncoding RNAs that play a vital role in gene regulation for many biological processes by binding target transcripts and marking them for degradation or post-transcriptional silencing. miRNAs undergo maturation within the nucleus beginning with cleavage ofprimary miRNA (pri-miRNA) by Drosha/DGCR8 to form premature miRNA (pre-miRNA). Whether this recognition relies more heavily on sequence motifs or structural features within the pri-miRNA is unknown. Algorithms based upon these potential recognition motifs predict many more miRNA sequences within the human genome than have been observed. To investigate the type of features thattarget a sequence for miRNA processing, we conduct a library screen of miRNA sequences within HEK293T cells to obtain a global view of miRNA processing. A reporter system consisting of GFP-P2A-puromycin and a mCherry—pri-miRNA fusion library was utilized. Library sequences that are processed by Drosha/DGCR8 lead to cleavage and degradation of the mCherry transcript and a decrease influorescence. These sequences are then analyzed for sequence motifs or structural features to elucidate how miRNAs are recognized and targeted for maturation.

Subjects/Keywords: Biochemistry; Biology; Genetics; Biogenesis; Cytometry; Drosha/DGCR8; Human; Library; microRNA

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6th Edition):

Ward, T. K. (2016). A global library screen of human primary microRNA processing utilizing a ratiometric fluorescence assay. (Thesis). UCLA. Retrieved from http://www.escholarship.org/uc/item/88s22248

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16th Edition):

Ward, Taylor Katherine. “A global library screen of human primary microRNA processing utilizing a ratiometric fluorescence assay.” 2016. Thesis, UCLA. Accessed August 24, 2019. http://www.escholarship.org/uc/item/88s22248.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7th Edition):

Ward, Taylor Katherine. “A global library screen of human primary microRNA processing utilizing a ratiometric fluorescence assay.” 2016. Web. 24 Aug 2019.

Vancouver:

Ward TK. A global library screen of human primary microRNA processing utilizing a ratiometric fluorescence assay. [Internet] [Thesis]. UCLA; 2016. [cited 2019 Aug 24]. Available from: http://www.escholarship.org/uc/item/88s22248.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Ward TK. A global library screen of human primary microRNA processing utilizing a ratiometric fluorescence assay. [Thesis]. UCLA; 2016. Available from: http://www.escholarship.org/uc/item/88s22248

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation


Uniwersytet im. Adama Mickiewicza w Poznaniu

2. Knop, Katarzyna. Biogeneza wybranych mikroRNA związanych z odpowiedzią rośliny na stresy abiotyczne .

Degree: 2017, Uniwersytet im. Adama Mickiewicza w Poznaniu

MikroRNA (miRNA) są zaangażowane w regulację ekspresji genów w komórce na poziomie potranskrypcyjnym. U roślin, za ich dojrzewanie odpowiedzialny jest tzw. kompleks mikroprocesora. Roślinne miRNA kodowane są przez geny MIR, posiadające skomplikowaną strukturę. U A. thaliana odkryto 29 miRNA zlokalizowanych w intronach genów kodujących białka lub niekodujące RNA, aczkolwiek mechanizm ich powstawania jest słabo poznany. W pierwszej części tej pracy doktorskiej skupiono się więc na jego analizie. Zaobserwowano, że w warunkach stresu abiotycznego podniesienie poziomu intronowej cząsteczki miR402 korelowało z obniżeniem wydajności splicingu intronu zawierającego miR402 oraz ze zwiększonym wyborem proksymalnego miejsca poliadenylacji. Ponadto, inaktywacja miejsca 5’ splicingowego (5’SS), która powodowała zahamowanie splicingu, skutkowała podniesieniem poziomu miR402 i częstszym wyborem proksymalnego miejsca poliA. Wykazano także, że lokalizacja pre-miRNA w stosunku do najbliższego miejsca 5’SS ma istotne znaczenie dla wydajności powstawania miR402 oraz, że oddziaływania pomiędzy maszynerią biogenezy i spliceosomem są kluczowe dla regulacji dojrzewania intronowych miRNA u roślin. Celem drugiej części tej pracy doktorskiej była analiza biogenezy cząsteczek miR319b i miR319b.2 Otrzymane wyniki wskazały, że powstawanie obu miRNA może być wzajemnie powiązane oraz, że oba miRNA mogą współkontrolować poziom swoich docelowych mRNA. Aczkolwiek do pełnego zrozumienia mechanizmu dojrzewania miR319b i miR319b.2, a także ich funkcji niezbędne jest przeprowadzenie dalszych eksperymentów. Advisors/Committee Members: Szweykowska-Kulińska, Zofia. Promotor (advisor).

Subjects/Keywords: A. thaliana; biogeneza mikroRNA; splicing; poliadenylacja; stres abiotyczny; microRNA biogenesis; polyadenylation; abiotic stress

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6th Edition):

Knop, K. (2017). Biogeneza wybranych mikroRNA związanych z odpowiedzią rośliny na stresy abiotyczne . (Doctoral Dissertation). Uniwersytet im. Adama Mickiewicza w Poznaniu. Retrieved from http://hdl.handle.net/10593/17526

Chicago Manual of Style (16th Edition):

Knop, Katarzyna. “Biogeneza wybranych mikroRNA związanych z odpowiedzią rośliny na stresy abiotyczne .” 2017. Doctoral Dissertation, Uniwersytet im. Adama Mickiewicza w Poznaniu. Accessed August 24, 2019. http://hdl.handle.net/10593/17526.

MLA Handbook (7th Edition):

Knop, Katarzyna. “Biogeneza wybranych mikroRNA związanych z odpowiedzią rośliny na stresy abiotyczne .” 2017. Web. 24 Aug 2019.

Vancouver:

Knop K. Biogeneza wybranych mikroRNA związanych z odpowiedzią rośliny na stresy abiotyczne . [Internet] [Doctoral dissertation]. Uniwersytet im. Adama Mickiewicza w Poznaniu; 2017. [cited 2019 Aug 24]. Available from: http://hdl.handle.net/10593/17526.

Council of Science Editors:

Knop K. Biogeneza wybranych mikroRNA związanych z odpowiedzią rośliny na stresy abiotyczne . [Doctoral Dissertation]. Uniwersytet im. Adama Mickiewicza w Poznaniu; 2017. Available from: http://hdl.handle.net/10593/17526


Kent State University

3. Mirihana Arachchilage, Gayan S. REGULATORY ROLES OF G-QUADRUPLEX IN microRNA PROCESSING AND mRNA TRANSLATION.

Degree: PhD, College of Arts and Sciences / Department of Chemistry, 2016, Kent State University

G-quadruplexes (GQs) are secondary structures that can be adopted by both DNA and RNA in the presence of monovalent cations and they are known to play important regulatory roles in a wide range of biological processes, such as, transcription, translation, splicing, mRNA localization, and telomere homeostasis. Although the functional roles of GQ structures have been extensively studied, the evolution of GQ structure is poorly understood. Thus, we investigated the evolutionary selection of GQ structures within the codon region (CDS) of mRNAs in a wide range of species and observed that the stable GQs are selected against within CDSs by synonymous codon usage. Furthermore, our results revealed context-dependent codon bias against the formation of stable GQs at the codon level resolution, thus delineating the evolutionary mechanism of GQ selection.Although a myriad of studies has reported the regulatory roles of GQs that are present in mRNAs, very little is known about the prevalence and functions of GQs in non-coding RNAs. We revealed the presence of GQ structures in precursor microRNA (pre-miRNA) and showed a regulatory role for GQs in miRNA biogenesis. Using a clinically important miRNA (miR-92b), we showed the existence of an equilibrium between GQ and the canonical stem-loop conformation which can regulate various stages of miRNA biogenesis such as nuclear export and Dicer-mediated maturation. Furthermore, we demonstrated that GQ structures can potentially be targeted in pre-miRNAs to treat diseases in which corresponding miRNAs are overexpressed. We showed that a rationally designed locked nucleic acid (LNA) oligonucleotide, which specifically binds with the GQ confirmation containing pre-miRNA can be used to treat non-small cell lung cancer where miR-92b is overexpressed.Although many different GQ binding ligands have been developed as therapeutics, the selectivity of these ligands is crucial to estimate the off-target effects. We developed an <i>in vitro </i>library screening approach to evaluate the selectivity of such ligands before proceeding into more detailed path of therapeutic development. Collectively, results from this set of studies not only elaborate our understanding of the structure-function relationship of RNAs and its clinical importance but also provide insight into the evolutionary selection of RNA secondary structures as regulatory elements. Advisors/Committee Members: Basu, Soumitra (Advisor).

Subjects/Keywords: Biochemistry; Cellular Biology; Chemistry; Molecular Biology; G-quadruplex; Dicer; pre-miRNA; microRNA; Codon bias; translation; microRNA biogenesis; library screening; LNA; lung cancer; RNA; RNA secondary structure; RNA switch; locked nucleic acids; miRNA therapeutics; in vitro selection; GQ switch

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6th Edition):

Mirihana Arachchilage, G. S. (2016). REGULATORY ROLES OF G-QUADRUPLEX IN microRNA PROCESSING AND mRNA TRANSLATION. (Doctoral Dissertation). Kent State University. Retrieved from http://rave.ohiolink.edu/etdc/view?acc_num=kent1469576783

Chicago Manual of Style (16th Edition):

Mirihana Arachchilage, Gayan S. “REGULATORY ROLES OF G-QUADRUPLEX IN microRNA PROCESSING AND mRNA TRANSLATION.” 2016. Doctoral Dissertation, Kent State University. Accessed August 24, 2019. http://rave.ohiolink.edu/etdc/view?acc_num=kent1469576783.

MLA Handbook (7th Edition):

Mirihana Arachchilage, Gayan S. “REGULATORY ROLES OF G-QUADRUPLEX IN microRNA PROCESSING AND mRNA TRANSLATION.” 2016. Web. 24 Aug 2019.

Vancouver:

Mirihana Arachchilage GS. REGULATORY ROLES OF G-QUADRUPLEX IN microRNA PROCESSING AND mRNA TRANSLATION. [Internet] [Doctoral dissertation]. Kent State University; 2016. [cited 2019 Aug 24]. Available from: http://rave.ohiolink.edu/etdc/view?acc_num=kent1469576783.

Council of Science Editors:

Mirihana Arachchilage GS. REGULATORY ROLES OF G-QUADRUPLEX IN microRNA PROCESSING AND mRNA TRANSLATION. [Doctoral Dissertation]. Kent State University; 2016. Available from: http://rave.ohiolink.edu/etdc/view?acc_num=kent1469576783

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