subject:(maternal aging)

1. Ivimey-Cook, Edward Richard. Experimental and comparative analyses of maternal age and senescence.

Degree: PhD, 2019, University of Edinburgh

► Senescence is often described as an age-related physiological deterioration accompanied with declining fertility and increasing mortality, and it is believed to be the result of… (more)

▼ Senescence is often described as an age-related physiological deterioration accompanied with declining fertility and increasing mortality, and it is believed to be the result of declining forces of natural selection. A manifestation of senescence that has attracted much recent interest is the detrimental effect of increasing maternal age acting on offspring traits. However, uncertainty arises when attempting to describe the prevalence and ubiquity of this third form of ageing and the evolutionary causes for diversity in ageing trajectories. Here I address the following questions: (1) How are maternal age effects distributed across taxa? And (2) Can an evolutionary perspective help us to understand the observed diversity in maternal age effects and demographic senescence? I addressed these through (i) a cross-fostering ageing experiment using a laboratory population of burying beetle, Nicrophorus vespilloides to decouple the separate effects of increasing pre- and postnatal maternal age, whilst accounting for the potential bias of selective disappearance. I found no evidence for maternal age effects or effects deriving from selective disappearance. These results suggest that current theory may be insufficient to account for the true diversity in ageing patterns. (ii) A meta-analytical review of maternal effect senescence to investigate the prevalence and diversity of maternal effect ageing patterns and the performance of an evolutionary model to predict observed patterns. We found taxa-wide evidence for maternal age effects on offspring survival. However the direction of these effects was based on phylogenetic constraints with laboratory and natural-mammal species showing a decline, but natural-bird species showing an ambiguous effect of maternal age. The evolutionary model was shown to improve in performance compared to evolution-agnostic demographic models when describing maternal effect ageing in natural populations. This result suggests an evolutionary cause to maternal effect senescence. (iii) Lastly, I performed a comparative analysis of vital rate selection across the tree of life. Using extensive existing databases of life history data coupled with predictions from two evolutionary theories, I derived correlations between predicted and observed vital rates across multiple animal species. I found that whilst natural selection had weak predictive power when describing patterns of mortality, age-specific fertility patterns showed extensive departures from evolutionary predictions. Additionally, I found that several biological processes were readily contributing to non-conformance of Hamilton-like ageing. Taken together, we provide convincing evidence to suggest that both natural selection and biological processes have helped shape the vast diversity of observed ageing rates that exist across the tree of life.

Subjects/Keywords: senescence; maternal effect; Nicrophorus vespilloides; ageing

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APA (6^th Edition):

Ivimey-Cook, E. R. (2019). Experimental and comparative analyses of maternal age and senescence. (Doctoral Dissertation). University of Edinburgh. Retrieved from http://hdl.handle.net/1842/35885

Chicago Manual of Style (16^th Edition):

Ivimey-Cook, Edward Richard. “Experimental and comparative analyses of maternal age and senescence.” 2019. Doctoral Dissertation, University of Edinburgh. Accessed April 16, 2021. http://hdl.handle.net/1842/35885.

MLA Handbook (7^th Edition):

Ivimey-Cook, Edward Richard. “Experimental and comparative analyses of maternal age and senescence.” 2019. Web. 16 Apr 2021.

Vancouver:

Ivimey-Cook ER. Experimental and comparative analyses of maternal age and senescence. [Internet] [Doctoral dissertation]. University of Edinburgh; 2019. [cited 2021 Apr 16]. Available from: http://hdl.handle.net/1842/35885.

Council of Science Editors:

Ivimey-Cook ER. Experimental and comparative analyses of maternal age and senescence. [Doctoral Dissertation]. University of Edinburgh; 2019. Available from: http://hdl.handle.net/1842/35885

McMaster University

2. Chan, Kaitlyn. CHARACTERIZING THE EFFECTS OF MATERNAL NUTRIENT RESTRICTION DURING PREGNANCY ON OFFSPRING OVARIAN FOLLICLE NUMBER, RECRUITMENT AND GROWTH FACTORS.

Degree: MSc, 2015, McMaster University

URL: http://hdl.handle.net/11375/18053

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The intrauterine environment induces developmental adaptations that impact health and disease risk later in life. Reproductive abnormalities are now included in the long list of… (more)

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The intrauterine environment induces developmental adaptations that impact health and disease risk later in life. Reproductive abnormalities are now included in the long list of health complications seen in offspring exposed to early life adversity including poor prenatal nutrition. Previous work has shown using a rat model, that offspring born to mothers that were nutrient restricted (UN) during pregnancy are growth restricted, enter puberty early, and as adults, display characteristics of early ovarian aging with reduced follicle number. This present study aimed to investigate whether key proteins involved in ovarian follicle recruitment and growth, including the PI3K/Akt pathway, may be impaired as a result of early life nutritional adversity. Maternal UN resulted in irregular estrous cyclicity due to persistent estrus, a significant decrease in young adult ovarian antral follicles, corpora lutea, and a significant increase in atretic follicles. A decrease in growing follicles in UN offspring appears to be due to lowered expression of granulosa-cell secreted growth factor IGF-1 in antral follicles, and increased expression of pro-apoptotic factor Casp3 in secondary follicles of young adult offspring born to UN dams. Changes in follicle signalling pathways were apparent before observing altered ovarian function. In UN prepubertal offspring, expression levels of IGF1-R and FSHR were lowered in secondary and antral follicles respectively. These growth factors may contribute to a decrease in PI3K/Akt activation as immunohistochemical staining revealed a decrease in pAkt immunolocalization in prepubertal antral follicles. Moreover, neonatal ovaries of UN offspring show decreased levels of immunopositive staining for AMHRII, a regulatory receptor of the ovarian reserve. This study demonstrates that maternal UN during pregnancy impacts ovarian function in female offspring as early as P65. Findings from this study provides a model of understanding mechanisms of follicle loss and reproductive dysfunction as a result of nutrient restriction during fetal life.

Thesis

Master of Science (MSc)

Advisors/Committee Members: Sloboda, Deborah, Biochemistry and Biomedical Sciences.

Subjects/Keywords: maternal nutrition; reproduction; pregnancy; IUGR; ovary; ovarian aging

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Chan, K. (2015). CHARACTERIZING THE EFFECTS OF MATERNAL NUTRIENT RESTRICTION DURING PREGNANCY ON OFFSPRING OVARIAN FOLLICLE NUMBER, RECRUITMENT AND GROWTH FACTORS. (Masters Thesis). McMaster University. Retrieved from http://hdl.handle.net/11375/18053

Chicago Manual of Style (16^th Edition):

Chan, Kaitlyn. “CHARACTERIZING THE EFFECTS OF MATERNAL NUTRIENT RESTRICTION DURING PREGNANCY ON OFFSPRING OVARIAN FOLLICLE NUMBER, RECRUITMENT AND GROWTH FACTORS.” 2015. Masters Thesis, McMaster University. Accessed April 16, 2021. http://hdl.handle.net/11375/18053.

MLA Handbook (7^th Edition):

Chan, Kaitlyn. “CHARACTERIZING THE EFFECTS OF MATERNAL NUTRIENT RESTRICTION DURING PREGNANCY ON OFFSPRING OVARIAN FOLLICLE NUMBER, RECRUITMENT AND GROWTH FACTORS.” 2015. Web. 16 Apr 2021.

Vancouver:

Chan K. CHARACTERIZING THE EFFECTS OF MATERNAL NUTRIENT RESTRICTION DURING PREGNANCY ON OFFSPRING OVARIAN FOLLICLE NUMBER, RECRUITMENT AND GROWTH FACTORS. [Internet] [Masters thesis]. McMaster University; 2015. [cited 2021 Apr 16]. Available from: http://hdl.handle.net/11375/18053.

Council of Science Editors:

Chan K. CHARACTERIZING THE EFFECTS OF MATERNAL NUTRIENT RESTRICTION DURING PREGNANCY ON OFFSPRING OVARIAN FOLLICLE NUMBER, RECRUITMENT AND GROWTH FACTORS. [Masters Thesis]. McMaster University; 2015. Available from: http://hdl.handle.net/11375/18053

Colorado State University

3. Ruggeri, Elena. Analysis of equine zygote development after intracytoplasmic sperm injection.

Degree: PhD, Biomedical Sciences, 2016, Colorado State University

URL: http://hdl.handle.net/10217/173335

► Intracytoplasmic sperm injection (ICSI) is an established and widely used method to achieve oocyte fertilization in equine reproductive assisted technologies. However, not all the oocytes… (more)

▼ Intracytoplasmic sperm injection (ICSI) is an established and widely used method to achieve oocyte fertilization in equine reproductive assisted technologies. However, not all the oocytes fertilized by ICSI undergo cleavage and develop into viable embryos. Limited knowledge on equine zygote development after ICSI is available, and reasons why developmental failure occurs after ICSI have been only partially studied and need further investigation. Fertility decline and early embryo loss is associated with maternal aging in the mare, and it is concomitant with reduced oocyte quality. Relatively little is known about the effect of maternal aging and zygote developmental failure or success in the mare. Effects of in vitro maturation of the oocyte or zygote development in the mare still need to be clarified and further studied. The overall objective of this dissertation was to study equine zygote development after ICSI using confocal microscopy. Objectives were to: (1) compare cytoskeletal and nuclear changes during progression of equine zygote development after ICSI for in vivo versus in vitro matured oocytes; (2) compare changes in cytoskeletal and chromosomal configurations after ICSI between oocytes from young and old mares to define maternal-aging related alterations; (3) determine cytoskeletal and nuclear alterations associated with fertilization failure in ICSI-produced presumptive zygotes in young and old mares; (4) determine cell-aging and cell donor-aging effects on cytoskeleton and chromatin configurations. Specifically, in our studies we evaluated the tubulin and actin cytoskeleton, chromatin, and kinetochores/centromeres. Immunostaining and confocal imaging of the equine zygotes was performed using a spinning disk confocal microscope. After ICSI, five distinct events of development were observed with no major differences over time whether oocytes matured in vivo or in vitro. Oocytes matured in vivo appeared to reach the pronucleus stage earlier after ICSI compared to in vitro matured oocytes. Abnormal phenotypes associated with fertilization failure were more significant in oocytes matured in vitro than in vivo. When ICSI was performed in oocytes from young and old mares, similar stages of zygote development were observed, and the number of zygotes reaching the pronucleus stage was similar between the two age groups. Nucleolus like bodies, sites of ribosomal RNA involved in embryonic genome activation, were observed only in zygotes at the pronucleus stage from young mares; no nucleolus-like bodies were observed in pronuclei of zygotes from old mares. Pronuclei morphology, based on CREST staining, and DNA localization, also differed between pronuclei of young and old mares. Actin vesicles were observed significantly more often within zygotes from old mares compared to young mares during all stages of zygote developmental progression. When potential zygotes were analyzed after failure of cleavage after ICSI, actin vesicles were greater in area, perimeter and number in oocytes from old mares than those… Advisors/Committee Members: Carnevale, Elaine (advisor), Clay, Colin (advisor), Albertini, David (committee member), DeLuca, Jennifer (committee member), Seidel, George (committee member).

Subjects/Keywords: cytoskeleton; intracytoplasmic sperm injection; zygote; equine; confocal microscopy; maternal aging

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Ruggeri, E. (2016). Analysis of equine zygote development after intracytoplasmic sperm injection. (Doctoral Dissertation). Colorado State University. Retrieved from http://hdl.handle.net/10217/173335

Chicago Manual of Style (16^th Edition):

Ruggeri, Elena. “Analysis of equine zygote development after intracytoplasmic sperm injection.” 2016. Doctoral Dissertation, Colorado State University. Accessed April 16, 2021. http://hdl.handle.net/10217/173335.

MLA Handbook (7^th Edition):

Ruggeri, Elena. “Analysis of equine zygote development after intracytoplasmic sperm injection.” 2016. Web. 16 Apr 2021.

Vancouver:

Ruggeri E. Analysis of equine zygote development after intracytoplasmic sperm injection. [Internet] [Doctoral dissertation]. Colorado State University; 2016. [cited 2021 Apr 16]. Available from: http://hdl.handle.net/10217/173335.

Council of Science Editors:

Ruggeri E. Analysis of equine zygote development after intracytoplasmic sperm injection. [Doctoral Dissertation]. Colorado State University; 2016. Available from: http://hdl.handle.net/10217/173335

University of the Western Cape

4. Adonis, Jihaan. The effect of maternal nicotine exposure on the alveolar wall composition during the phases of lung development .

Degree: 2015, University of the Western Cape

URL: http://hdl.handle.net/11394/4704

► Cigarette smoking is one of the foremost causes of chronic obstructive pulmonary diseases such as emphysema and chronic bronchitis, and although it is the most… (more)

▼ Cigarette smoking is one of the foremost causes of chronic obstructive pulmonary diseases such as emphysema and chronic bronchitis, and although it is the most preventable causes of death, it accounts for approximately 6 million deaths worldwide each year. Cigarette smoking during pregnancy and lactation remains one of the primary modifiable risk factors for undesirable fetal, obstetrical, and developmental outcomes. Consequently, the offspring of the smoking mother is exposed to nicotine via the blood and the milk of the mother. As a result, nicotine interacts with the developing offspring and therefore interferes with normal fetal lung development. Maternal smoking during gestation and lactation has been associated with both short and long term health risks ranging from intrauterine growth restriction to physiological abnormalities. Maternal smoking has also been strongly linked to an increased risk for pulmonary diseases and respiratory morbidity in the offspring of the smoking mother. The main objectives of this study were to determine the effects of maternal nicotine exposure during gestation and lactation on the alveolar wall composition during lung development in the offspring; if maternal nicotine exposure during gestation and lactation induces premature cellular senescence in the lungs of the offspring; to clarify the role of pulmonary fibroblasts in premature senescence; and to establish whether tomato juice supplementation will prevent premature aging in the lungs of rats that were exposed to nicotine via the placenta and mother’s milk. From the data generated in this study it was evident that maternal nicotine exposure during gestation and lactation compromises the gas exchange function of the lungs of the F1 offspring. This was prevented by supplementing the mother’s diet with tomato juice which is then received by the offspring via the placenta and mother’s milk. This is conceivably achieved by maintaining the oxidant-anti-oxidant ratio of the mother and of the developing fetus and neonate, thereby averting premature senescence caused by nicotine exposure. Moreover, the present study also demonstrates that a decrease in fibroblast density is associated with emphysematous-like lesions in the lungs of the nicotine exposed F1 progeny. Since pulmonary fibroblasts are chief contributors to the extracellular matrix of the lungs, involved in alveolar multiplication and regeneration; premature aging or cessation of the metabolically active fibroblasts largely contributes to diminished lung structure and function. Advisors/Committee Members: Maritz, G.S (advisor), De Kock, M (advisor).

Subjects/Keywords: Cigarette smoking; Pulmonary fibroblasts nicotine; Maternal exposure; Lung development; Tomato juice; Premature aging

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APA (6^th Edition):

Adonis, J. (2015). The effect of maternal nicotine exposure on the alveolar wall composition during the phases of lung development . (Thesis). University of the Western Cape. Retrieved from http://hdl.handle.net/11394/4704

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Adonis, Jihaan. “The effect of maternal nicotine exposure on the alveolar wall composition during the phases of lung development .” 2015. Thesis, University of the Western Cape. Accessed April 16, 2021. http://hdl.handle.net/11394/4704.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Adonis, Jihaan. “The effect of maternal nicotine exposure on the alveolar wall composition during the phases of lung development .” 2015. Web. 16 Apr 2021.

Vancouver:

Adonis J. The effect of maternal nicotine exposure on the alveolar wall composition during the phases of lung development . [Internet] [Thesis]. University of the Western Cape; 2015. [cited 2021 Apr 16]. Available from: http://hdl.handle.net/11394/4704.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Adonis J. The effect of maternal nicotine exposure on the alveolar wall composition during the phases of lung development . [Thesis]. University of the Western Cape; 2015. Available from: http://hdl.handle.net/11394/4704

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of the Western Cape

5. Mutemwa, Muyunda. Premature aging of the lungs of the offspring induced by maternal nicotine exposure during gestation and lactation: protective effects of tomato juice .

Degree: 2012, University of the Western Cape

URL: http://hdl.handle.net/11394/2957

► exposed to nicotine through the blood and the milk of the mother. Nicotine is thus expected to interact with the developing fetus and the offspring… (more)

▼ exposed to nicotine through the blood and the milk of the mother. Nicotine is thus expected to interact with the developing fetus and the offspring of mothers who smoke or use NRT for smoking cessation, resulting in the interference with normal fetal and neonatal lung development. Maternal cigarette smoke or nicotine exposure produces adverse effects in the lungs of offspring, these include; intrauterine growth retardation, low birth weight, premature birth, reduced pulmonary function at birth, and a high occurrence of respiratory illnesses after birth. This study aimed at investigating the effects of maternal nicotine exposure during gestation and lactation on lung development in the offspring; to establish whether tomato juice can have protective effects on the fetal lung development and function in the offspring; and to determine if nicotine cases premature aging of the lungs of the offspring. It was therefore shown that maternal exposure to nicotine during gestation and lactation ad no significant effect on the growth parameters of the offspring. Maternal nicotine exposure during gestation and lactation had no effect on the growth parameters of the offspring, but resulted in compromised lung structure and function. The morphometric results demonstrated decrease in alveolar number, increase in alveolar size, and decrease in lung parenchyma of the nicotine exposed animals showing a gradual deterioration of the lung parenchyma. Structural alterations include emphysematous lesions, where the latter was accompanied by an increase in alveolar size (Lm), and a decrease in the tissue volume of the lung parenchyma. Thickening of alveolar walls was also evident and serves as an indication of remodeling of the extracellular matrix, also a characteristic of emphysema. A consequence of the gradual deterioration of the lung parenchyma is a decrease in the alveolar surface area available for gas exchange. The present study showed that the emphysematous lesions were conceivably a result of a reduced rate of cell proliferation accompanied by the increase in senescent cells numbers in the alveolar walls of the exposed offspring. The data of this study suggests that maternal nicotine exposure during gestation and lactation induces premature aging of the lungs of the offspring rendering the lungs of the offspring more susceptible to disease later in life. Since these structural changes occurred later in the life of the offspring and long after nicotine withdrawal, it is suggested that it is programmed during gestation and lactation. Smoking and NRT result in an increased load of oxidants in the mother and fetus. It also reduces the level of anti-oxidants and thereby compromising the ability of the mother to protect the fetus. It is hypothesized that this oxidant-antioxidant imbalance will program the lungs to age prematurely. The supplementation of the mother’s diet with tomato juice, rich in lycopene, other anti-oxidants such as vitamin C, as well as phytonutrients protected the lungs of the offspring against the… Advisors/Committee Members: Maritz, Gert S (advisor).

Subjects/Keywords: Tobacco smoking; Nicotine; Maternal exposure; Lung development; Tomato Juice; Lycopene; Oxidative stress; Emphysema; Premature aging; Transgeneration

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APA (6^th Edition):

Mutemwa, M. (2012). Premature aging of the lungs of the offspring induced by maternal nicotine exposure during gestation and lactation: protective effects of tomato juice . (Thesis). University of the Western Cape. Retrieved from http://hdl.handle.net/11394/2957

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Mutemwa, Muyunda. “Premature aging of the lungs of the offspring induced by maternal nicotine exposure during gestation and lactation: protective effects of tomato juice .” 2012. Thesis, University of the Western Cape. Accessed April 16, 2021. http://hdl.handle.net/11394/2957.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Mutemwa, Muyunda. “Premature aging of the lungs of the offspring induced by maternal nicotine exposure during gestation and lactation: protective effects of tomato juice .” 2012. Web. 16 Apr 2021.

Vancouver:

Mutemwa M. Premature aging of the lungs of the offspring induced by maternal nicotine exposure during gestation and lactation: protective effects of tomato juice . [Internet] [Thesis]. University of the Western Cape; 2012. [cited 2021 Apr 16]. Available from: http://hdl.handle.net/11394/2957.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Mutemwa M. Premature aging of the lungs of the offspring induced by maternal nicotine exposure during gestation and lactation: protective effects of tomato juice . [Thesis]. University of the Western Cape; 2012. Available from: http://hdl.handle.net/11394/2957

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

6. Dadarwal, Dinesh. FACTORS AFFECTING MATURATION OF BOVINE OOCYTES; HORMONAL ENVIRONMENT, FOLLICULAR AND MATERNAL AGING.

Degree: 2012, University of Saskatchewan

URL: http://hdl.handle.net/10388/ETD-2012-11-792

► Hormonal environment in which follicle grows has been shown to affect the oocyte competence. Our objective was to identify factors that affect oocyte competence and… (more)

▼ Hormonal environment in which follicle grows has been shown to affect the oocyte competence. Our objective was to identify factors that affect oocyte competence and characterize the structural and functional changes induced by these factors. Fertility was compared in cattle following alterations in levels of progesterone and length of proestrus during dominant follicle growth. We hypothesized that subluteal-phase progesterone will mitigate the effect of a shorter proestrus on pregnancy rates. A shorter duration of proestrus during a fixed-time AI protocol in cattle resulted in a smaller preovulatory follicle, smaller and less functional CL with lower progesterone secretion, and lower fertility (P<0.01). A subluteal-phase progesterone milieu during ovulatory follicle growth induced higher pregnancy rates (P<0.01) and compensated for the effect of a short proestrus on pregnancy rates. Organelle behavior was characterized in oocytes obtained from follicles at different phases of dominant follicle growth. We hypothesized that ooplasmic organelles undergo changes in population and spatial distribution in a phase-specific manner. The growing phase oocytes have least area of mitochondria in contact with lipid droplets (P=0.04) and a peripheral distribution of lipids compared to an even distribution in oocytes from other phases. The regression phase oocytes showed an increase in mitochondrial number (P=0.03) and even distribution of mitochondria compared to peripheral in other phases. Moreover, oocytes from regression phase had higher (P<0.01) lipid content per unit volume of oocyte than other phases. Effect of follicular aging on nuclear maturation rates and, size and distribution of lipid droplets and mitochondria in in vivo matured oocytes were compared. We hypothesized that follicular aging after FSH starvation will result in maturation failure with accumulation of larger lipid droplets and altered distribution of mitochondria as compared to superstimulation with continued FSH support (4-d and 7-d). A 7-d FSH protocol resulted in greater proportion (P<0.01) of mature oocytes compared to other groups. FSH starvation lead to more poor quality oocytes that had ATP contents similar to short FSH group. Further, organization of mitochondria as intense and bigger clusters (P=0.01) alongwith increased size of lipid droplet (P=0.03) within the oocytes from FSH starvation group might indicate atresia. Effect of maternal aging on mitochondrial numbers, distribution and ATP content of in vivo matured bovine oocytes was studied. We hypothesized that in vivo matured oocytes from old cows will have reduced number of mitochondria, altered distribution of mitochondria and decreased the ATP content compared to those from young cows. Maternally aged oocytes had significantly less ATP content (P=0.01) although mitochondrial population and distribution pattern did not differ. Advisors/Committee Members: Singh, Jaswant, Adams, Gregg P., Lessard, Carl, Tikoo, Suresh K., Muir, Gillian.

Subjects/Keywords: Oocyte maturation; follicular aging; maternal aging; bovine model

…to undergo mutations due to oxidative stress especially with aging (Croteau and Bohr…

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APA (6^th Edition):

Dadarwal, D. (2012). FACTORS AFFECTING MATURATION OF BOVINE OOCYTES; HORMONAL ENVIRONMENT, FOLLICULAR AND MATERNAL AGING. (Thesis). University of Saskatchewan. Retrieved from http://hdl.handle.net/10388/ETD-2012-11-792

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Dadarwal, Dinesh. “FACTORS AFFECTING MATURATION OF BOVINE OOCYTES; HORMONAL ENVIRONMENT, FOLLICULAR AND MATERNAL AGING.” 2012. Thesis, University of Saskatchewan. Accessed April 16, 2021. http://hdl.handle.net/10388/ETD-2012-11-792.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Dadarwal, Dinesh. “FACTORS AFFECTING MATURATION OF BOVINE OOCYTES; HORMONAL ENVIRONMENT, FOLLICULAR AND MATERNAL AGING.” 2012. Web. 16 Apr 2021.

Vancouver:

Dadarwal D. FACTORS AFFECTING MATURATION OF BOVINE OOCYTES; HORMONAL ENVIRONMENT, FOLLICULAR AND MATERNAL AGING. [Internet] [Thesis]. University of Saskatchewan; 2012. [cited 2021 Apr 16]. Available from: http://hdl.handle.net/10388/ETD-2012-11-792.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Dadarwal D. FACTORS AFFECTING MATURATION OF BOVINE OOCYTES; HORMONAL ENVIRONMENT, FOLLICULAR AND MATERNAL AGING. [Thesis]. University of Saskatchewan; 2012. Available from: http://hdl.handle.net/10388/ETD-2012-11-792

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

7. Aledani, Tamadir Hamid Wadi. Expression des ARNm et des microARN dans les cellules de cumulus humains : impact de l'âge maternel : Expression of mRNAs and microRNAs in the human cumulus cells : impact of maternal age.

Degree: Docteur es, Biologie Santé, 2015, Montpellier

URL: http://www.theses.fr/2015MONTT011

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L'ovocyte se développe au sein d'un follicule, en contact étroit avec des cellules d'origine somatique, les cellules de cumulus (CC). Ces deux types cellulaires communiquent… (more)

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L'ovocyte se développe au sein d'un follicule, en contact étroit avec des cellules d'origine somatique, les cellules de cumulus (CC). Ces deux types cellulaires communiquent entre eux via des jonctions intercellulaires, permettant ainsi la régulation et la coordination du métabolisme pendant le développement et la maturation de l'ovocyte. Notre hypothèse est que l'expression et la régulation des gènes dans les CC joue un rôle crucial dans des fonctions essentielles pour la croissance de l'ovocyte et l'acquisition de sa compétence. Mes travaux de thèse comportent deux parties. Dans la première partie nous avons utilisé le séquençage haut débit pour examiner le répertoire des microARN (communément appelés miRNA) dans les cellules de cumulus et dans l'ovocyte. Les miRNA, séquences d'ARN non codantes dont la longueur varie entre 19 et 25 nucléotides, ont émergé récemment comme régulateurs majeurs de nombreux processus biologiques, dont le vieillissement. Nous avons identifié 32 miRNA spécifiquement dans les cellules de cumulus humains et seulement 3 dans l'ovocyte MII. Dans la seconde partie de nos travaux, nous avons analysé l'impact de l'âge maternel sur l'expression des gènes dans les cellules de cumulus. Alors qu'une baisse de la compétence de l'ovocyte avec l'avancement de l'âge maternel est bien établie, les bases moléculaires de ce phénomène demeurent peu connues. Dans une première étape pour aborder cette question, nous avons utilisé des puces à ADN pour analyser les profils d'expression des gènes des CC en fonction de l'âge maternel. De façon remarquable l'âge maternel impacte significativement l'expression de gènes qui sont critiques pour la maturation de l'ovocyte tels que les gènes impliqués dans l'angiogenèse, les voies de signalisation de TGF-ß et de l'insuline. Par l'utilisation d'outils bioinformatiques, nous avons aussi identifié des miRNA potentiels régulateurs de gènes impliqués dans des processus ou des voies impactés par l'âge ; ils pourraient constituer de nouveaux biomarqueurs pour prédire un vieillissement ovarien prématuré ainsi que la qualité et la compétence de l'ovocyte.

The oocyte develops into a follicle where it is in close contact with cumulus cells (CCs), of somatic origin. The two cell types undergo a bidirectional communication via gap junctions, which results in the regulation and coordination of the metabolism during oocyte development and maturation. We assume that gene expression and regulation in the CCs play a crucial role in functions that are essential for oocyte growth and competence acquisition. The present study may be subdivided in two parts. In the first part we used deep sequencing to investigate the repertoire of miRNAs in the cumulus cells and the oocyte. MicroRNAs that are noncoding RNA sequences whose length is approximately 19-25 nucleotides have emerged as important regulators in many biological processes including aging. Our data showed that 32 miRNAs were specifically expressed in human cumulus cells while only 3 miRNAs were identified in MII human oocyte. The…

Advisors/Committee Members: Hamamah, Samir (thesis director), Aït Ahmed, Ounissa (thesis director).

Subjects/Keywords: Ovocyte humain; Cellules de cumulus; Âge maternel; Transcriptome; Micro-ARN; Séquençage haut débit; Human oocyte; Cumulus cells; Maternal aging; Transcriptome; MicroRNAs; Deep sequencing

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Aledani, T. H. W. (2015). Expression des ARNm et des microARN dans les cellules de cumulus humains : impact de l'âge maternel : Expression of mRNAs and microRNAs in the human cumulus cells : impact of maternal age. (Doctoral Dissertation). Montpellier. Retrieved from http://www.theses.fr/2015MONTT011

Chicago Manual of Style (16^th Edition):

Aledani, Tamadir Hamid Wadi. “Expression des ARNm et des microARN dans les cellules de cumulus humains : impact de l'âge maternel : Expression of mRNAs and microRNAs in the human cumulus cells : impact of maternal age.” 2015. Doctoral Dissertation, Montpellier. Accessed April 16, 2021. http://www.theses.fr/2015MONTT011.

MLA Handbook (7^th Edition):

Aledani, Tamadir Hamid Wadi. “Expression des ARNm et des microARN dans les cellules de cumulus humains : impact de l'âge maternel : Expression of mRNAs and microRNAs in the human cumulus cells : impact of maternal age.” 2015. Web. 16 Apr 2021.

Vancouver:

Aledani THW. Expression des ARNm et des microARN dans les cellules de cumulus humains : impact de l'âge maternel : Expression of mRNAs and microRNAs in the human cumulus cells : impact of maternal age. [Internet] [Doctoral dissertation]. Montpellier; 2015. [cited 2021 Apr 16]. Available from: http://www.theses.fr/2015MONTT011.

Council of Science Editors:

Aledani THW. Expression des ARNm et des microARN dans les cellules de cumulus humains : impact de l'âge maternel : Expression of mRNAs and microRNAs in the human cumulus cells : impact of maternal age. [Doctoral Dissertation]. Montpellier; 2015. Available from: http://www.theses.fr/2015MONTT011

University of Georgia

8. Priest, Nicholas Kiefer. Maternal age effects on offspring longevity in Drosophila melanogaster.

Degree: 2014, University of Georgia

URL: http://hdl.handle.net/10724/20417

► Maternal effects may play a crucial role in the evolution of aging. We conducted several experiments with different strains of the fruit fly, Drosophila melanogaster,… (more)

Subjects/Keywords: Maternal effects; Parental investment; Lansing effects; Costs of reproduction; Senesence; Mortality; Evolution of aging; Carry-over effects; Cross-generational effect; Life history trade-offs.

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APA (6^th Edition):

Priest, N. K. (2014). Maternal age effects on offspring longevity in Drosophila melanogaster. (Thesis). University of Georgia. Retrieved from http://hdl.handle.net/10724/20417

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Priest, Nicholas Kiefer. “Maternal age effects on offspring longevity in Drosophila melanogaster.” 2014. Thesis, University of Georgia. Accessed April 16, 2021. http://hdl.handle.net/10724/20417.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Priest, Nicholas Kiefer. “Maternal age effects on offspring longevity in Drosophila melanogaster.” 2014. Web. 16 Apr 2021.

Vancouver:

Priest NK. Maternal age effects on offspring longevity in Drosophila melanogaster. [Internet] [Thesis]. University of Georgia; 2014. [cited 2021 Apr 16]. Available from: http://hdl.handle.net/10724/20417.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Priest NK. Maternal age effects on offspring longevity in Drosophila melanogaster. [Thesis]. University of Georgia; 2014. Available from: http://hdl.handle.net/10724/20417

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

The Ohio State University

9. Reynolds, Tamara. Transgenerational effects of maternal age on fertility of offspring.

Degree: MS, Genetic Counseling, 2017, The Ohio State University

URL: http://rave.ohiolink.edu/etdc/view?acc_num=osu1492115742061456

► Infertility, defined as the failure to achieve a clinical pregnancy after 12 months or more of regular unprotected sexual intercourse (Zegers-Hochschild et al, 2009), affects… (more)

▼ Infertility, defined as the failure to achieve a clinical pregnancy after 12 months or more of regular unprotected sexual intercourse (Zegers-Hochschild et al, 2009), affects 9-18% of the population (Aghajanova, 2016). Numerous factors have been linked to infertility (Hanson et al, 2016) with increasing maternal age believed to have the strongest association (American College of Obstetricians and Gynecologists Committee, 2014). Ten to thirty percent of infertility remains unexplained (Quass & Hansen, 2016). More women are having children later in life due to educational and career pursuits despite confirmed increase in infertility (Martin et al, 2015) (Sauer, 2015). Women are born with all the egg cells they will ever have and as women age, their egg cells age along with them. An increase in egg cell nondisjunction, changes to the oocyte nuclear genome, changes to its mitochondrial genome, telomere length alterations, and epigenetic modifications in oocytes all are thought to influence age related infertility. While it is clear that the age of a woman (G1) is associated with her own fertility, what is not known is whether the age of the woman (G1) at the birth of her daughter (G2) is correlated with the fertility of the daughter (G2). During fetal development of G2 which happens in the milieu of the G1 environment, all of the egg cells that will become G3 are formed. Epigenetic modifications to the G3 egg cell which may compromise its integrity are a potential mechanism for disruption to the future fertility of G2. Associations between age of G1 and outcomes in both G2 and G3 have been found.To test the impact of G1 age on fertility of G2, we conducted an online survey of over 3000 women. Survey questions assessed participant fertility, ages of participants and their parents, family reproductive history, and long-term health outcome of offspring. Analyses showed that women (G2) born to teenage mothers (G1) were 40% more likely to experience infertility than women (G2) whose mothers (G1) were 20-29 (95% CI 1.08-1.81; p-value= 0.01). This effect persisted after controlling for participant (G2) age. No significant difference in fertility was found in participants (G2) with older mothers (G1); however, only a small number of participants (G2) had mothers (G1) over age 40, which may not have sufficiently powered the analyses for that group. The results of our study did not support the hypothesis that advanced age of mother (G1) at the birth of her daughter (G2) is associated with infertility in the daughter (G2). Additional studies should be done to confirm the association of G1 age of less than 20 with increased infertility as this may have reproductive implications. Advisors/Committee Members: Toland, Amanda (Advisor).

Subjects/Keywords: Public Health; Medicine; Individual and Family Studies; Health Sciences; Health Care; Gynecology; Genetics; Aging; Transgenerational effects of maternal age, fertility of offspring, infertility

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Reynolds, T. (2017). Transgenerational effects of maternal age on fertility of offspring. (Masters Thesis). The Ohio State University. Retrieved from http://rave.ohiolink.edu/etdc/view?acc_num=osu1492115742061456

Chicago Manual of Style (16^th Edition):

Reynolds, Tamara. “Transgenerational effects of maternal age on fertility of offspring.” 2017. Masters Thesis, The Ohio State University. Accessed April 16, 2021. http://rave.ohiolink.edu/etdc/view?acc_num=osu1492115742061456.

MLA Handbook (7^th Edition):

Reynolds, Tamara. “Transgenerational effects of maternal age on fertility of offspring.” 2017. Web. 16 Apr 2021.

Vancouver:

Reynolds T. Transgenerational effects of maternal age on fertility of offspring. [Internet] [Masters thesis]. The Ohio State University; 2017. [cited 2021 Apr 16]. Available from: http://rave.ohiolink.edu/etdc/view?acc_num=osu1492115742061456.

Council of Science Editors:

Reynolds T. Transgenerational effects of maternal age on fertility of offspring. [Masters Thesis]. The Ohio State University; 2017. Available from: http://rave.ohiolink.edu/etdc/view?acc_num=osu1492115742061456

10. Daniela Silva Martinez. Investigação do comprimento telomérico em famílias com vários afetados pelo transtorno bipolar.

Degree: 2018, University of São Paulo

URL: http://www.teses.usp.br/teses/disponiveis/5/5142/tde-06042018-121637/

► INTRODUÇÃO: O Transtorno Bipolar (TB) é um transtorno psiquiátrico crônico e debilitante e sua etiologia e patologia ainda não são completamente conhecidos, apesar de um… (more)

▼ INTRODUÇÃO: O Transtorno Bipolar (TB) é um transtorno psiquiátrico crônico e debilitante e sua etiologia e patologia ainda não são completamente conhecidos, apesar de um componente genético importante ser evidenciado em estudos de família, adoção e gêmeos. Recentemente, o TB tem sido relacionado a um processo de envelhecimento acelerado, com alguns estudos mostrando telômeros encurtados nesta população. O objetivo do presente estudo foi investigar a associação entre o comprimento telomérico, um dos parâmetros do processo de envelhecimento celular, com a ausência ou presença de TB em famílias com muitos membros afetados, além de associar a sintomatologia clínica e outras variáveis a esse parâmetro. Procurou-se também avaliar as influências genéticas e ambientais sobre o comprimento telomérico nessas famílias, estimando-se a herdabilidade desta característica. MÉTODOS: O comprimento telomérico (T) foi mensurado em uma amostra de 143 indivíduos de 22 famílias (60 deles com TB), em relação a um gene de cópia única (S) - beta-globina, através do método de PCR (Polymerase Chain Reaction) em tempo real quantitativo, no qual forneceu uma proporção do número de cópias de T por S (razão T/S). Considerando a estrutura familiar na análise estatística foi ajustado para cada análise o modelo misto poligênico. RESULTADOS: O efeito do TB no comprimento dos telômeros foi pequeno, não tendo sido observada uma associação estatisticamente significante entre TB e comprimento telomérico quando comparado com familiares saudáveis (p > 0,05). No entanto, observou-se associação do comprimento telomérico à covariável ideação suicida (p = 0,02) e à interação entre ideação suicida e curso da doença (p = 0,02). Associação do comprimento telomérico com idade materna e TB também foi observada (p < 0,05). Por fim, estimou-se em 68% a herdabilidade do comprimento telomérico nas 22 famílias do estudo. CONCLUSÕES: A teoria do envelhecimento acelerado em TB, vista pela óptica do comprimento dos telômeros, não pôde ser confirmada no presente estudo, pois não foi encontrada diferença no comprimento telomérico entre indivíduos saudáveis e com TB nas famílias. Por outro lado, covariáveis que indicam gravidade da doença, como a ideação suicida e a interação entre ideação suicida e curso da doença foram associadas ao comprimento telomérico (p < 0,05), ou seja, um encurtamento telomérico foi correlacionado à gravidade clínica do TB. Associação do comprimento telomérico com idade materna e TB (p < 0,05) sugeriu que a idade materna avançada não só pode ser um marcador de longevidade, como também o fenótipo TB pareceu reforçar essa condição. Por fim, a alta herdabilidade estimada do comprimento telomérico (0,68) revelou uma importante variabilidade genética desse fenótipo entre as famílias do estudo. Em súmula, este é o primeiro estudo que relatou uma associação entre ideação suicida, curso da doença, idade materna e comprimento telomérico em famílias com vários membros afetados pelo TB. Outras investigações independentes são necessárias para confirmar esses… Advisors/Committee Members: Homero Pinto Vallada Filho, Orestes Vicente Forlenza, Daniel Shikanai Kerr, Vanessa de Jesus Rodrigues de Paula.

Subjects/Keywords: Comprimento telomérico; Curso da doença; Envelhecimento celular; Herdabilidade; Idade materna; Ideação suicida; Telômero; Transtornos do humor; Cell aging; Course of disorder; Heritability; Maternal age; Mood disorders; Suicidal ideation; Telomere; Telomere length

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Martinez, D. S. (2018). Investigação do comprimento telomérico em famílias com vários afetados pelo transtorno bipolar. (Masters Thesis). University of São Paulo. Retrieved from http://www.teses.usp.br/teses/disponiveis/5/5142/tde-06042018-121637/

Chicago Manual of Style (16^th Edition):

Martinez, Daniela Silva. “Investigação do comprimento telomérico em famílias com vários afetados pelo transtorno bipolar.” 2018. Masters Thesis, University of São Paulo. Accessed April 16, 2021. http://www.teses.usp.br/teses/disponiveis/5/5142/tde-06042018-121637/.

MLA Handbook (7^th Edition):

Martinez, Daniela Silva. “Investigação do comprimento telomérico em famílias com vários afetados pelo transtorno bipolar.” 2018. Web. 16 Apr 2021.

Vancouver:

Martinez DS. Investigação do comprimento telomérico em famílias com vários afetados pelo transtorno bipolar. [Internet] [Masters thesis]. University of São Paulo; 2018. [cited 2021 Apr 16]. Available from: http://www.teses.usp.br/teses/disponiveis/5/5142/tde-06042018-121637/.

Council of Science Editors:

Martinez DS. Investigação do comprimento telomérico em famílias com vários afetados pelo transtorno bipolar. [Masters Thesis]. University of São Paulo; 2018. Available from: http://www.teses.usp.br/teses/disponiveis/5/5142/tde-06042018-121637/

11. Rizzo, Marilena. Aneuploidy in horse oocytes and embryos; how late is too late? The 'fertility clock' doesn't only tick for career women.

Degree: 2020, University Utrecht

URL: https://dspace.library.uu.nl/handle/1874/398804 ; URN:NBN:NL:UI:10-1874-398804 ; 10.33540/218 ; 1874/398804 ; urn:isbn:9789463809337 ; URN:NBN:NL:UI:10-1874-398804 ; https://dspace.library.uu.nl/handle/1874/398804

► Advanced mare age is known to be associated with reduced fertility and increased incidence of early pregnancy loss (EPL). Aberrations of embryonic chromosome number (aneuploidy)… (more)

▼ Advanced mare age is known to be associated with reduced fertility and increased incidence of early pregnancy loss (EPL). Aberrations of embryonic chromosome number (aneuploidy) appear to be the primary contributor to this age-related increase in EPL. Age-related aneuploidy is the primary cause of developmental arrest, implantation failure, miscarriage and congenital birth defects in human pregnancy, and is mostly associated with loss of inter-chromosomal cohesion, abnormalities in chromosome-spindle interaction, and lack of stringency of the Spindle Assembly Checkpoint (SAC) mechanism. The aim of this thesis was to evaluate the effect of advanced mare age on the incidence of aneuploidy in in vitro matured oocytes, and to investigate the contributions to aneuploidy of the loss of chromosomal centromeric cohesion and of an abnormal SAC activity. In particular, we evaluated the effect of advanced mare age on gene expression for selected cohesin components, and on the incidence of aneuploidy and the strength of chromosome centromere cohesion by, respectively, counting the number of chromatid-kinetochore units and measuring the inter-kinetochore distance (iKD). Afterwards, we investigated the gene expression for the main SAC components in in vitro matured oocytes from young and old mares, and on the basis of these results, we investigated the function of MPS1 and AURKB/C during spindle assembly by analysing spindle morphology and chromosome alignment of in vitro matured MII oocytes from old and young mares, after nocodazole-induced microtubule depolymerization and spindle reassembly in the presence of specific MPS1 and AURKB/C inhibitors. Finally, we indirectly evaluated the incidence on aneuploidy in in vitro produced (IVP) and in vivo derived embryos, by evaluating presence of micronuclei and analysing nuclear morphology. At the same time, we investigated whether a correlation exists between bright field morphological characteristics of IVP embryos, the incidence of nuclear abnormalities and the likelihood of pregnancy after transfer. We showed that advanced mare age is associated with an increased incidence of chromosome misalignment and aneuploidy in in vitro matured oocytes. We found indeed premature separation and random segregation of the sister chromatids during the first meiotic division, which may originate from a weakened chromosomal centromeric cohesion, possibly explained by the increased iKD in oocytes from old mares. Moreover, MII oocytes from old mares showed a reduced mRNA expression for the SAC components MPS1, SPC25 and AURKC, and a higher incidence of spindle abnormalities after exposure to the MPS1 inhibitor CPD5. The defective function of the SAC protein MPS1 in oocytes from aged mares, may contribute to spindle instability which, in turn, could result in mis-segregation leading to aneuploidy. In addition, in vitro culture of horse embryos favours micronucleus formations, considered to be a proxy for mitotic aneuploidy. However, of all the bright field morphological characteristics, only the time of… Advisors/Committee Members: Stout, T.A.E., De Ruijter - Villani, M..

Subjects/Keywords: maternal aging; aneuploidy; oocytes; embryos; cohesion loss; inter-kinetochore distance; spindle assembly checkpoint; chromosome alignment; spindle instability; micronuclei

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Rizzo, M. (2020). Aneuploidy in horse oocytes and embryos; how late is too late? The 'fertility clock' doesn't only tick for career women. (Doctoral Dissertation). University Utrecht. Retrieved from https://dspace.library.uu.nl/handle/1874/398804 ; URN:NBN:NL:UI:10-1874-398804 ; 10.33540/218 ; 1874/398804 ; urn:isbn:9789463809337 ; URN:NBN:NL:UI:10-1874-398804 ; https://dspace.library.uu.nl/handle/1874/398804

Chicago Manual of Style (16^th Edition):

Rizzo, Marilena. “Aneuploidy in horse oocytes and embryos; how late is too late? The 'fertility clock' doesn't only tick for career women.” 2020. Doctoral Dissertation, University Utrecht. Accessed April 16, 2021. https://dspace.library.uu.nl/handle/1874/398804 ; URN:NBN:NL:UI:10-1874-398804 ; 10.33540/218 ; 1874/398804 ; urn:isbn:9789463809337 ; URN:NBN:NL:UI:10-1874-398804 ; https://dspace.library.uu.nl/handle/1874/398804.

MLA Handbook (7^th Edition):

Rizzo, Marilena. “Aneuploidy in horse oocytes and embryos; how late is too late? The 'fertility clock' doesn't only tick for career women.” 2020. Web. 16 Apr 2021.

Vancouver:

Rizzo M. Aneuploidy in horse oocytes and embryos; how late is too late? The 'fertility clock' doesn't only tick for career women. [Internet] [Doctoral dissertation]. University Utrecht; 2020. [cited 2021 Apr 16]. Available from: https://dspace.library.uu.nl/handle/1874/398804 ; URN:NBN:NL:UI:10-1874-398804 ; 10.33540/218 ; 1874/398804 ; urn:isbn:9789463809337 ; URN:NBN:NL:UI:10-1874-398804 ; https://dspace.library.uu.nl/handle/1874/398804.

Council of Science Editors:

Rizzo M. Aneuploidy in horse oocytes and embryos; how late is too late? The 'fertility clock' doesn't only tick for career women. [Doctoral Dissertation]. University Utrecht; 2020. Available from: https://dspace.library.uu.nl/handle/1874/398804 ; URN:NBN:NL:UI:10-1874-398804 ; 10.33540/218 ; 1874/398804 ; urn:isbn:9789463809337 ; URN:NBN:NL:UI:10-1874-398804 ; https://dspace.library.uu.nl/handle/1874/398804

Open Access Theses and Dissertations