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You searched for subject:(mammalian phospholipase C). One record found.

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Boston College

1. Cai, Jingfei. Probing the Membrane Association Mechanisms for Pulmonary Collectins and Mammalian Phospholipase C.

Degree: PhD, Chemistry, 2013, Boston College

Peripheral proteins from mammals often exhibit multi-domain structures and require metal ions such as calcium as co-factors. This dissertation investigates two types of such proteins  – pulmonary collectins (surfactant proteins A and D) and phosphatidylinositol-specific phospholipase C (PLC) delta1  – and their interactions with model membranes. One approach to work around the complexity brought upon by such multi-domain protein structure is to use a truncated construct or an isolated single domain. For pulmonary collectins, homotrimers consisting of the neck domain and the carbohydrate recognition domain were used in a novel NMR assay for better understanding of their lipid-specific interactions with the membranes. For PLC delta1, we were particularly interested in the role of the EF-hand domain. The isolated EF-hand domain of PLC delta1 was first used to characterize its interactions with membranes and identify key residues responsible for such interactions. These key residues in the N terminal lobe of the EF-hand domain, either cationic or hydrophobic, were then found to affect the hydrolysis activity of the full-length enzyme. A common role for this region of the PLC in facilitating proper membrane association was thus proposed. Advisors/Committee Members: Mary F. Roberts (Thesis advisor), Eranthie Weerapana (Thesis advisor).

Subjects/Keywords: EF-hand domain; mammalian phospholipase C; model membrane; surfactant protein A; surfactant protein D

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6th Edition):

Cai, J. (2013). Probing the Membrane Association Mechanisms for Pulmonary Collectins and Mammalian Phospholipase C. (Doctoral Dissertation). Boston College. Retrieved from http://dlib.bc.edu/islandora/object/bc-ir:101804

Chicago Manual of Style (16th Edition):

Cai, Jingfei. “Probing the Membrane Association Mechanisms for Pulmonary Collectins and Mammalian Phospholipase C.” 2013. Doctoral Dissertation, Boston College. Accessed January 24, 2020. http://dlib.bc.edu/islandora/object/bc-ir:101804.

MLA Handbook (7th Edition):

Cai, Jingfei. “Probing the Membrane Association Mechanisms for Pulmonary Collectins and Mammalian Phospholipase C.” 2013. Web. 24 Jan 2020.

Vancouver:

Cai J. Probing the Membrane Association Mechanisms for Pulmonary Collectins and Mammalian Phospholipase C. [Internet] [Doctoral dissertation]. Boston College; 2013. [cited 2020 Jan 24]. Available from: http://dlib.bc.edu/islandora/object/bc-ir:101804.

Council of Science Editors:

Cai J. Probing the Membrane Association Mechanisms for Pulmonary Collectins and Mammalian Phospholipase C. [Doctoral Dissertation]. Boston College; 2013. Available from: http://dlib.bc.edu/islandora/object/bc-ir:101804

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