subject:(lymphocytes)

1. Al-Omairi, Jaafar Ghadeer Khudhair. Functional study of lymphoid specific enhancers : Etude fonctionnelle des enhancers lymphoides.

Degree: Docteur es, Génétique, 2017, Aix Marseille Université

URL: http://www.theses.fr/2017AIXM0474

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Les amplificateurs (aussi appelés par le terme anglais enhancers) ont été initialement identifiés comme des séquences d'ADN agissant en cis qui augmentent la transcription d'une… (more)

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Les amplificateurs (aussi appelés par le terme anglais enhancers) ont été initialement identifiés comme des séquences d'ADN agissant en cis qui augmentent la transcription d'une manière qui est indépendante de leur orientation et de leur distance par rapport au site d’initiation de la transcription. En outre, les gènes d'identité cellulaire sont souvent associés à des regroupements ou clusters d’enhancers, structures également appelées super-enhancers, censés assurer une régulation correcte de l'expression des gènes tout au long du développement et de la différenciation des cellules. Pour mieux comprendre la régulation des gènes à partir de ces réseaux régulateurs complexes, nous avons étudié la régulation du gène Ikzf1 qui code pour un facteur de transcription essentiel à la différenciation lymphoïde et également impliqué dans la leucémogenèse. En combinant différents types de données épigénomiques, nous avons privilégié l’étude d’un élément enhancer situé à 120 kb en amont du gène Ikzf1. Nous avons trouvé que la délétion de l'enhancer IkE120 entraine une réduction significative de l'ARNm d’Ikzf1. Cependant, nous avons observé que la transcription immature ainsi que l’usage des promoteurs et exons alternatifs d’Ikzf1 sont différemment affectée dans les cellules délitées par IKE120. Ces résultats semblent indiquer que l'élément IkE120 pourrait avoir des fonctions supplémentaires au-delà de la seule régulation de l'initiation de la transcription.

Enhancers were originally identified as cis-acting DNA sequences that increase transcription in a manner that is independent of their orientation and distance relative to the RNA start site. In addition, cell identity genes are often associated with cluster of enhancers, also termed super-enhancers, which are believed to ensure proper regulation of gene expression throughout cell development and differentiation. To better understand gene regulation based on these complex regulatory networks, we studied the regulation of the Ikzf1 gene which encoded for a lymphoid-specific transcription factor essential for lymphoid differentiation and also involved in leukemogenesis. By combining different epigenomics data sets we prioritize an enhancer element located 120 kb upstream the IKZF1 gene. We found that deletion of the E120 enhancer resulted in significant reduction of Ikzf1 mRNA. However, we observed that immature transcription, promoter and exon usage were differentially affected in the IKE120-deleted cells. The results indicated that E120 element might have additional functions over solely regulating transcription initiation. We suggest that expression of some tissue-specific and cell identity genes might, at least partially, be regulated at the level of mRNA maturation and that components of enhancer’s clusters are directly involved in this process.

Advisors/Committee Members: Spicuglia, Salvatore (thesis director).

Subjects/Keywords: Promoteur; Enhancer; Lymphocytes; Promoter; Enhancer; Lymphocytes; 572

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APA (6^th Edition):

Al-Omairi, J. G. K. (2017). Functional study of lymphoid specific enhancers : Etude fonctionnelle des enhancers lymphoides. (Doctoral Dissertation). Aix Marseille Université. Retrieved from http://www.theses.fr/2017AIXM0474

Chicago Manual of Style (16^th Edition):

Al-Omairi, Jaafar Ghadeer Khudhair. “Functional study of lymphoid specific enhancers : Etude fonctionnelle des enhancers lymphoides.” 2017. Doctoral Dissertation, Aix Marseille Université. Accessed January 20, 2021. http://www.theses.fr/2017AIXM0474.

MLA Handbook (7^th Edition):

Al-Omairi, Jaafar Ghadeer Khudhair. “Functional study of lymphoid specific enhancers : Etude fonctionnelle des enhancers lymphoides.” 2017. Web. 20 Jan 2021.

Vancouver:

Al-Omairi JGK. Functional study of lymphoid specific enhancers : Etude fonctionnelle des enhancers lymphoides. [Internet] [Doctoral dissertation]. Aix Marseille Université 2017. [cited 2021 Jan 20]. Available from: http://www.theses.fr/2017AIXM0474.

Council of Science Editors:

Al-Omairi JGK. Functional study of lymphoid specific enhancers : Etude fonctionnelle des enhancers lymphoides. [Doctoral Dissertation]. Aix Marseille Université 2017. Available from: http://www.theses.fr/2017AIXM0474

Cornell University

2. Prieto, Jennifer. Equine B Lymphopoiesis And Cd5+ B Lymphocytes.

Degree: PhD, Immunology, 2015, Cornell University

URL: http://hdl.handle.net/1813/41107

► The development of the humoral immune system starts during gestation with the production of two major types of B cells: B1 and B2. B2 cells… (more)

▼ The development of the humoral immune system starts during gestation with the production of two major types of B cells: B1 and B2. B2 cells are the classic players in the adaptive humoral immunity, while B1 cells are considered components of innate immunity and have important developmental, phenotypic, and functional differences with B2 cells. B1 cells are predominant early in ontology in mice and humans; therefore, my studies of B cell development in the horse began in the primary lymphoid organs of the fetus. In the first part of my study, I tested how primary lymphoid tissues of the equine fetus were equipped to support B cell hematopoiesis and immunoglobulin (Ig) diversity of the pre-immune repertoire. I found that the equine fetal liver and the bone marrow are active sites of hematopoiesis based on the expression of signature mRNA (c-KIT, CD34, IL7R, CXCL12, IRF8, PU.1, PAX5, NOTCH1, GATA1, CEBPA) and protein markers (CD34, CD19, IgM, CD3, CD4, CD5, CD8, CD11b, CD172A) of hematopoietic development and leukocyte differentiation molecules, respectively at approximately 100 days of gestation (DG). Immunoglobulin V(D)J segments were sequenced in primary lymphoid organs of the equine fetus and adult horse, revealing that some aspects of Ig diversity in the adult were established in the fetus (similar heavy chain VDJ segment utilization, CDR3 lengths), while others were developmentally programmed (lambda light chain segment utilization, sequence diversity). These data suggest that the B cells produced in the liver and bone marrow of the equine fetus generate a wide repertoire of pre-immune Igs for protection, and the more diverse use of different lambda variable gene segments in fetal life may provide the neonate an opportunity to respond to a wider range of antigens at birth. In the second part of my study, I tested aspects of ontogeny, phenotype, and function of equine CD5hi B cells, putative B1-like cells. The expression of CD5 can distinguish murine B1a from B2 cells, and my study tested how this marker could distinguish B1-like cells in the horse. I learned that CD5hi B cells were more frequent early in development and in the peritoneal cavity. CD5hi cells more frequently coexpressed CD2, CD21, and CD11b than CD5lo B cells in the peripheral blood when measured with flow cytometry, but had similar mRNA expression of B1 and B2 signature genes including DGKA, FGL2, PAX5, and IGHM when measured with qRTPCR. Sequencing of lambda light chain segments revealed that CD5hi and CD5lo B cells generate similar levels of immunoglobulin diversity; however, CD5hi B cells more frequently bound to fluorescence-labeled phosphorylcholine and secreted IL-10 in vitro. CD5hi B cells also represented a greater proportion of remaining B cells in horses with common variable immunodeficiency. Taken together, the results of my study show that equine CD5hi B cells share some of the same characteristics attributed to B1 cells in other species, and may be explored for immune protection in patients with humoral immunodeficiency. Advisors/Committee Members: Flaminio,Maria Julia Bevilaqua Felippe (chair), Appleton,Judith Ann (committee member), Marquis,Helene (committee member), Schimenti,John C. (committee member), Fortier,Lisa Ann (committee member).

Subjects/Keywords: Equine; B Lymphocytes; Development

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APA (6^th Edition):

Prieto, J. (2015). Equine B Lymphopoiesis And Cd5+ B Lymphocytes. (Doctoral Dissertation). Cornell University. Retrieved from http://hdl.handle.net/1813/41107

Chicago Manual of Style (16^th Edition):

Prieto, Jennifer. “Equine B Lymphopoiesis And Cd5+ B Lymphocytes.” 2015. Doctoral Dissertation, Cornell University. Accessed January 20, 2021. http://hdl.handle.net/1813/41107.

MLA Handbook (7^th Edition):

Prieto, Jennifer. “Equine B Lymphopoiesis And Cd5+ B Lymphocytes.” 2015. Web. 20 Jan 2021.

Vancouver:

Prieto J. Equine B Lymphopoiesis And Cd5+ B Lymphocytes. [Internet] [Doctoral dissertation]. Cornell University; 2015. [cited 2021 Jan 20]. Available from: http://hdl.handle.net/1813/41107.

Council of Science Editors:

Prieto J. Equine B Lymphopoiesis And Cd5+ B Lymphocytes. [Doctoral Dissertation]. Cornell University; 2015. Available from: http://hdl.handle.net/1813/41107

University of Texas Southwestern Medical Center

3. Paek, So Yeon. Soluble Peptide Treatment Reverses CD8 T Cell-Induced Disease in a Mouse Model of Spontaneous Tissue-Selective Autoimmunity.

Degree: 2011, University of Texas Southwestern Medical Center

URL: http://hdl.handle.net/2152.5/927

► Autoimmunity is a complex process that involves recognition of self antigens by autoreactive T cells or tissue targeting by autoantibodies produced by B cells. Specific… (more)

Subjects/Keywords: Peptide Fragments; T-Lymphocytes; Autoimmunity

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APA (6^th Edition):

Paek, S. Y. (2011). Soluble Peptide Treatment Reverses CD8 T Cell-Induced Disease in a Mouse Model of Spontaneous Tissue-Selective Autoimmunity. (Thesis). University of Texas Southwestern Medical Center. Retrieved from http://hdl.handle.net/2152.5/927

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Paek, So Yeon. “Soluble Peptide Treatment Reverses CD8 T Cell-Induced Disease in a Mouse Model of Spontaneous Tissue-Selective Autoimmunity.” 2011. Thesis, University of Texas Southwestern Medical Center. Accessed January 20, 2021. http://hdl.handle.net/2152.5/927.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Paek, So Yeon. “Soluble Peptide Treatment Reverses CD8 T Cell-Induced Disease in a Mouse Model of Spontaneous Tissue-Selective Autoimmunity.” 2011. Web. 20 Jan 2021.

Vancouver:

Paek SY. Soluble Peptide Treatment Reverses CD8 T Cell-Induced Disease in a Mouse Model of Spontaneous Tissue-Selective Autoimmunity. [Internet] [Thesis]. University of Texas Southwestern Medical Center; 2011. [cited 2021 Jan 20]. Available from: http://hdl.handle.net/2152.5/927.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Paek SY. Soluble Peptide Treatment Reverses CD8 T Cell-Induced Disease in a Mouse Model of Spontaneous Tissue-Selective Autoimmunity. [Thesis]. University of Texas Southwestern Medical Center; 2011. Available from: http://hdl.handle.net/2152.5/927

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Université Laval

4. Gervais-St-Amour, Catherine. Étude de la différenciation des lymphocytes B mémoires en milieu sans sérum.

Degree: 2013, Université Laval

URL: http://hdl.handle.net/20.500.11794/24508

► Les infections opportunistes sont l’une des principales causes de mortalité associées à la greffe de cellules souches. Afin d’aider à reconstituer le système immunitaire des… (more)

Subjects/Keywords: Lymphocytes B – Différenciation; Plasmocytes

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APA (6^th Edition):

Gervais-St-Amour, C. (2013). Étude de la différenciation des lymphocytes B mémoires en milieu sans sérum. (Thesis). Université Laval. Retrieved from http://hdl.handle.net/20.500.11794/24508

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Gervais-St-Amour, Catherine. “Étude de la différenciation des lymphocytes B mémoires en milieu sans sérum.” 2013. Thesis, Université Laval. Accessed January 20, 2021. http://hdl.handle.net/20.500.11794/24508.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Gervais-St-Amour, Catherine. “Étude de la différenciation des lymphocytes B mémoires en milieu sans sérum.” 2013. Web. 20 Jan 2021.

Vancouver:

Gervais-St-Amour C. Étude de la différenciation des lymphocytes B mémoires en milieu sans sérum. [Internet] [Thesis]. Université Laval; 2013. [cited 2021 Jan 20]. Available from: http://hdl.handle.net/20.500.11794/24508.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Gervais-St-Amour C. Étude de la différenciation des lymphocytes B mémoires en milieu sans sérum. [Thesis]. Université Laval; 2013. Available from: http://hdl.handle.net/20.500.11794/24508

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Université Laval

5. Guérard, Simon. Kératinocytes : cellules instigatrices de l'inflammation dans le psoriasis : étude sur la capacité des kératinocytes psoriasiques à activer in vitro les lymphocytes T et les neutrophiles.

Degree: 2011, Université Laval

URL: http://hdl.handle.net/20.500.11794/23652

► Le rôle primordial des cellules de la peau dans la réponse immunitaire est désormais bien établi. Cependant, pourraient-elles également être responsables de l'activation du système… (more)

Subjects/Keywords: Kératinocytes; Psoriasis; Lymphocytes T; Neutrophiles

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APA (6^th Edition):

Guérard, S. (2011). Kératinocytes : cellules instigatrices de l'inflammation dans le psoriasis : étude sur la capacité des kératinocytes psoriasiques à activer in vitro les lymphocytes T et les neutrophiles. (Thesis). Université Laval. Retrieved from http://hdl.handle.net/20.500.11794/23652

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Guérard, Simon. “Kératinocytes : cellules instigatrices de l'inflammation dans le psoriasis : étude sur la capacité des kératinocytes psoriasiques à activer in vitro les lymphocytes T et les neutrophiles.” 2011. Thesis, Université Laval. Accessed January 20, 2021. http://hdl.handle.net/20.500.11794/23652.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Guérard, Simon. “Kératinocytes : cellules instigatrices de l'inflammation dans le psoriasis : étude sur la capacité des kératinocytes psoriasiques à activer in vitro les lymphocytes T et les neutrophiles.” 2011. Web. 20 Jan 2021.

Vancouver:

Guérard S. Kératinocytes : cellules instigatrices de l'inflammation dans le psoriasis : étude sur la capacité des kératinocytes psoriasiques à activer in vitro les lymphocytes T et les neutrophiles. [Internet] [Thesis]. Université Laval; 2011. [cited 2021 Jan 20]. Available from: http://hdl.handle.net/20.500.11794/23652.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Guérard S. Kératinocytes : cellules instigatrices de l'inflammation dans le psoriasis : étude sur la capacité des kératinocytes psoriasiques à activer in vitro les lymphocytes T et les neutrophiles. [Thesis]. Université Laval; 2011. Available from: http://hdl.handle.net/20.500.11794/23652

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Texas Southwestern Medical Center

6. Belkaya, Serkan. Characterization of Stress Responsive MicroRNAs and Their Roles in T Cell Development.

Degree: 2014, University of Texas Southwestern Medical Center

URL: http://hdl.handle.net/2152.5/1422

► Physiological stress evokes rapid changes in both the innate and adaptive immune responses. Immature αβ T cells developing in the thymus are particularly sensitive to… (more)

▼ Physiological stress evokes rapid changes in both the innate and adaptive immune responses. Immature αβ T cells developing in the thymus are particularly sensitive to stress, with infections and/or exposure to lipopolysaccharide or glucocorticoids eliciting a rapid apoptotic program. MicroRNAs (miRs) are short, non-coding RNAs that play critical roles in the immune system by targeting diverse mRNAs. We hypothesized that a subset of thymically encoded miRs would be stress responsive and modulate thymopoiesis. Thymic miR profiling revealed 18 distinct miRs that are dysregulated more than 1.5-fold in response to lipopolysaccharide or the synthetic glucocorticoid dexamethasone. These stress-responsive miRs are dynamically regulated in distinct thymocyte subsets. We utilized both transgenic and gene-targeting approaches to study the impact of these miRs on thymopoiesis under normal and stress conditions. MiR-181d is the most down-regulated thymic miR in response to stress. The over-expression of miR-181d in developing thymocytes reduced the number of immature CD4+CD8+ thymocytes. Lipopolysaccharide or dexamethasone injections caused a 4-fold greater loss of these cells than in the wild type controls. The targeted elimination of miR-181d resulted in a thymus stress-responsiveness similar to wild-type mice, suggesting a functional redundancy between miR-181 family members. Gene expression comparisons further indicated that miR-181d affects a number of stress, metabolic, and signaling pathways. These findings demonstrate that selected miRs enhance stress-mediated thymic involution in vivo. MiR-185, another stress-responsive miR in murine thymus, is haploinsufficient in almost all individuals with 22q11.2 deletion/DiGeorge syndrome that can present with immune, cardiac, parathyroid, and psychological problems. The molecular targets of miR-185 in thymocytes are unknown. Transgenic expression of miR-185 attenuated thymopoiesis at the TCRβ-selection checkpoint and during positive selection. This caused a peripheral T cell lymphopenia. Mzb1, NFATc3, and Camk4 were identified as novel miR-185 targets. Elevations in miR-185 enhanced TCR-dependent intracellular calcium levels, while a knockdown of miR-185 diminished these calcium responses. These effects concur with reductions in Mzb1, an endoplasmic reticulum calcium regulator. Consistent with the haploinsufficiency of miR-185, Mzb1 levels were elevated in thymocyte extracts from several 22q11.2 deletion/DiGeorge syndrome patients. These findings indicate that miR-185 regulates T cell development through its targeting of several mRNAs including Mzb1. Advisors/Committee Members: Hooper, Lora V., van Oers, Nicolai S. C., Chen, Zhijian J., Yarovinsky, Felix, Liou, Jen.

Subjects/Keywords: MicroRNAs; Stress, Physiological; T-Lymphocytes

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APA (6^th Edition):

Belkaya, S. (2014). Characterization of Stress Responsive MicroRNAs and Their Roles in T Cell Development. (Thesis). University of Texas Southwestern Medical Center. Retrieved from http://hdl.handle.net/2152.5/1422

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Belkaya, Serkan. “Characterization of Stress Responsive MicroRNAs and Their Roles in T Cell Development.” 2014. Thesis, University of Texas Southwestern Medical Center. Accessed January 20, 2021. http://hdl.handle.net/2152.5/1422.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Belkaya, Serkan. “Characterization of Stress Responsive MicroRNAs and Their Roles in T Cell Development.” 2014. Web. 20 Jan 2021.

Vancouver:

Belkaya S. Characterization of Stress Responsive MicroRNAs and Their Roles in T Cell Development. [Internet] [Thesis]. University of Texas Southwestern Medical Center; 2014. [cited 2021 Jan 20]. Available from: http://hdl.handle.net/2152.5/1422.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Belkaya S. Characterization of Stress Responsive MicroRNAs and Their Roles in T Cell Development. [Thesis]. University of Texas Southwestern Medical Center; 2014. Available from: http://hdl.handle.net/2152.5/1422

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Texas Southwestern Medical Center

7. Roybal, Kole Thomas 1982-. Actin Regulatory Dynamics Required for T Cell Activation: A Quantitative and Systems-Level Perspective.

Degree: 2013, University of Texas Southwestern Medical Center

URL: http://hdl.handle.net/2152.5/ETD-UTSWMED-2013-05-91

► T cell activation occurs through interaction with an antigen-presenting cell (APC). Upon activation, signaling ensues with the coordination of dozens of diverse signaling molecules in… (more)

▼ T cell activation occurs through interaction with an antigen-presenting cell (APC). Upon activation, signaling ensues with the coordination of dozens of diverse signaling molecules in space and time, a feature of cell signaling we call ‘spatiotemporal patterning’. We performed a systems-scale analysis of the spatiotemporal patterning of T cell signaling and have found that it is highly diverse. Over 50 signaling sensors were imaged in live primary T cells activated with APCs under various physiological stimulation conditions, and no two signaling intermediates showed the same dynamic localization. The activation environment controlled spatiotemporal features of T cell signaling and specific spatiotemporal features correlated with efficient T cell activation. To identify underlying cell biological mechanisms controlling spatiotemporal organization of signaling, we complimented our live cell imaging with microscopy across multiple scales and identified a dense transient F-actin network that extends from a highly interdigitated T cell:APC interface several micrometers deep into the T cell lamellum. Systems-scale imaging revealed a large network of proximal T cell signaling intermediates that localized to the lamellal actin network and shared the spatial, temporal, and mobility features of F-actin. Interference with lamellal actin dynamics modulated the activity of the associated proteins and impaired IL-2 production. These data strongly suggest that the transient deep F-actin network by controlling lamellal localization modulates the activity of a substantial part of the T cell signal transduction system. As a next step in understanding how spatiotemporal dynamics of signaling controls T cell activation, we have developed a quantitative 4D analysis approach for signaling networks and coupled it with traditional cell biological techniques to uncover higher order mechanisms of the control of actin dynamics by CD28 co-stimulation during T cell activation. A group of nine actin regulatory proteins that mediate actin polymerization, capping, and severing were assessed and CD28 co-stimulation was required for their sustained activity at the T cell:APC interface. WAVE2 and Cofilin were especially sensitive to blockade of CD28 signaling. Functional relevance of the loss of WAVE2 and Cofilin enrichment was shown by the treatment of T cells with constitutively active Rac1 and Cofilin, which bypassed the requirement of co-stimulation for normal actin dynamics and AKT activation. This study highlights how a systems analysis of actin regulation could identify mechanisms that are inaccessible to more traditional single protein/gene approaches. Advisors/Committee Members: Ward, E. Sally, Wülfing, Christoph, Rosen, Michael K., Pasare, Chandrashekhar.

Subjects/Keywords: Actins; Signal Transduction; T-Lymphocytes

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APA (6^th Edition):

Roybal, K. T. 1. (2013). Actin Regulatory Dynamics Required for T Cell Activation: A Quantitative and Systems-Level Perspective. (Thesis). University of Texas Southwestern Medical Center. Retrieved from http://hdl.handle.net/2152.5/ETD-UTSWMED-2013-05-91

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Roybal, Kole Thomas 1982-. “Actin Regulatory Dynamics Required for T Cell Activation: A Quantitative and Systems-Level Perspective.” 2013. Thesis, University of Texas Southwestern Medical Center. Accessed January 20, 2021. http://hdl.handle.net/2152.5/ETD-UTSWMED-2013-05-91.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Roybal, Kole Thomas 1982-. “Actin Regulatory Dynamics Required for T Cell Activation: A Quantitative and Systems-Level Perspective.” 2013. Web. 20 Jan 2021.

Vancouver:

Roybal KT1. Actin Regulatory Dynamics Required for T Cell Activation: A Quantitative and Systems-Level Perspective. [Internet] [Thesis]. University of Texas Southwestern Medical Center; 2013. [cited 2021 Jan 20]. Available from: http://hdl.handle.net/2152.5/ETD-UTSWMED-2013-05-91.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Roybal KT1. Actin Regulatory Dynamics Required for T Cell Activation: A Quantitative and Systems-Level Perspective. [Thesis]. University of Texas Southwestern Medical Center; 2013. Available from: http://hdl.handle.net/2152.5/ETD-UTSWMED-2013-05-91

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

8. Ghannam, Soufiane. Les lymphocytes Th17 humains : modulation de leur fonction effectrice par les cellules souches mésenchymateuses et caractérisation de leurs propriétés migratoires : Human Th17 lymphocytes : modulation of their effector function by mesenchymal stem cells and characterization of their migratory properties.

Degree: Docteur es, Biologie cellulaire, 2010, Université Montpellier I

URL: http://www.theses.fr/2010MON1T023

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Les lymphocytes Th17 forment une population de cellules T CD4+ pro-inflammatoires, impliqués non seulement dans l'élimination d'agents pathogènes, mais ayant aussi un rôle délétère dans… (more)

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Les lymphocytes Th17 forment une population de cellules T CD4+ pro-inflammatoires, impliqués non seulement dans l'élimination d'agents pathogènes, mais ayant aussi un rôle délétère dans l'induction de maladies inflammatoires chroniques. Ils expriment spécifiquement le récepteur de chimiokines CCR6, qui a pour ligand le CCL20 mais aussi les β-defensine-1, 2 et 3, peptides ayant une activité antimicrobienne. Les cellules souches mésenchymateuses (CSMs) représentent une population cellulaire hétérogène exerçant diverses propriétés immunomodulatrices.Les résultats obtenus dans ce travail de thèse montrent que l'environnement inflammatoire contribue à augmenter l'adhésion des lymphocytes Th17 aux CSMs, et qu'elle est régulée par l'interaction du CCR6 avec ses ligands ; que les CSMs exercent, en partie via la sécrétion de PGE2, des effets anti-inflammatoires en faisant acquérir un phénotype régulateur aux lymphocytes Th17 différenciés, soulignant ainsi la plasticité de ces derniers.De plus, nous avons montré que les lymphocytes Th17 activés par l'antigène produisent du CCL20 et induisent, via la production de l'IL-17 et de l'IL-22, la sécrétion d'hBD-2, mais pas celle des hBD-1 et 3, par des kératinocytes épidermiques humains et de la peau reconstituée; que le CCL20, ainsi que la hBD-2, induisent l'arrêt de ces cellules sur l'endothélium enflammé in vitro en conditions de cisaillement. Finalement, l'activation spécifique d'antigène des lymphocytes Th17 entraîne une perte de l'expression de CCR6, ce qui provoque ainsi un état transitoire de non réponse à une nouvelle stimulation de ces cellules avec les ligands de CCR6, permettant leur migration ultérieure hors du tissu enflammé.

Th17 cells form a population CD4+ T cells with strong pro-inflammatory properties that are not only involved in the clearance of pathogens, but also play a deleterious role of in the pathogenesis of inflammatory disease. Th17 cells specifically express CCR6, a chemokine receptor that binds to its unique chemokine ligand, CCL20, as well as to human β-defensin (hBD)-1, 2 and 3, peptides with anti-microbial activity. Mesenchymal stem cells (MSC) represent a heterogenous population that exert broad immunomodulatory effects.The results from the studies carried out during this thesis show that the inflammatory environment contributes to increased adhesion of Th17 cells to MSCs, which is mediated via the interaction of CCR6 with its ligands, and that MSCs exert, in part via the secretion of PGE2, anti-inflammatory effects through the induction of a T regulatory cell phenotype in fully differentiated tissue-infiltrating Th17 cells, thereby underscoring the plasticity of the latter cells.Furthermore, the results show that antigen-activated Th17 cells produce CCL20 and induce, via the production of both IL-17 and IL-22, the secretion of hBD-2, but not 1 and 3, by normal human epidermal keratinocytes and reconstituted skin, and that CCL20, as well as hBD-2, induce arrest of these cells onto inflamed…

Advisors/Committee Members: Yssel, Hans (thesis director).

Subjects/Keywords: Cellules souches mésenchymateuses; Lymphocytes Th17; Lymphocytes T régulateurs; Inflammation; Mesenchymal stem cells; Th17 lymphocytes; T regulatory lymphocytes; Inflamamation

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APA (6^th Edition):

Ghannam, S. (2010). Les lymphocytes Th17 humains : modulation de leur fonction effectrice par les cellules souches mésenchymateuses et caractérisation de leurs propriétés migratoires : Human Th17 lymphocytes : modulation of their effector function by mesenchymal stem cells and characterization of their migratory properties. (Doctoral Dissertation). Université Montpellier I. Retrieved from http://www.theses.fr/2010MON1T023

Chicago Manual of Style (16^th Edition):

Ghannam, Soufiane. “Les lymphocytes Th17 humains : modulation de leur fonction effectrice par les cellules souches mésenchymateuses et caractérisation de leurs propriétés migratoires : Human Th17 lymphocytes : modulation of their effector function by mesenchymal stem cells and characterization of their migratory properties.” 2010. Doctoral Dissertation, Université Montpellier I. Accessed January 20, 2021. http://www.theses.fr/2010MON1T023.

MLA Handbook (7^th Edition):

Ghannam, Soufiane. “Les lymphocytes Th17 humains : modulation de leur fonction effectrice par les cellules souches mésenchymateuses et caractérisation de leurs propriétés migratoires : Human Th17 lymphocytes : modulation of their effector function by mesenchymal stem cells and characterization of their migratory properties.” 2010. Web. 20 Jan 2021.

Vancouver:

Ghannam S. Les lymphocytes Th17 humains : modulation de leur fonction effectrice par les cellules souches mésenchymateuses et caractérisation de leurs propriétés migratoires : Human Th17 lymphocytes : modulation of their effector function by mesenchymal stem cells and characterization of their migratory properties. [Internet] [Doctoral dissertation]. Université Montpellier I; 2010. [cited 2021 Jan 20]. Available from: http://www.theses.fr/2010MON1T023.

Council of Science Editors:

Ghannam S. Les lymphocytes Th17 humains : modulation de leur fonction effectrice par les cellules souches mésenchymateuses et caractérisation de leurs propriétés migratoires : Human Th17 lymphocytes : modulation of their effector function by mesenchymal stem cells and characterization of their migratory properties. [Doctoral Dissertation]. Université Montpellier I; 2010. Available from: http://www.theses.fr/2010MON1T023

Université Laval

9. Bonnaure, Guillaume. Les plasmocytes et leur niche : étude de la génération de plasmocytes humains in vitro.

Degree: 2016, Université Laval

URL: http://hdl.handle.net/20.500.11794/27053

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Tableau d'honneur de la Faculté des études supérieures et postdoctorales, 2016-2017

Chez l’humain, les lymphocytes B mémoires IgG+ et IgA+ sont des cellules clés de… (more)

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Tableau d'honneur de la Faculté des études supérieures et postdoctorales, 2016-2017

Chez l’humain, les lymphocytes B mémoires IgG+ et IgA+ sont des cellules clés de l’immunité humorale. Ces cellules mémoires sont maintenues à long-terme dans notre organisme. Elles représentent une défense rapide et efficace contre toutes les infections que nous avons déjà vaincues pendant notre vie. Ces cellules mémoires qui rencontrent à nouveau leur antigène se différencient rapidement en plasmocytes à courte vie, et permettent la sécrétion massive d’immunoglobuline (Ig). La contrepartie mémoire de ces cellules sont les plasmocytes à longue vie qui sont présents dans les niches de la moelle osseuse et y sécrètent en permanence des anticorps protecteurs qui circulent dans le sang. Ces cellules sécrétrices peuvent avoir une durée de vie allant de dizaines d’années à la vie entière de l’individu. Les patients qui reçoivent des traitements de chimiothérapie ou de radiothérapie sont privés de ces cellules mémoires détruites par ces traitements au même titre que les cellules cancéreuses. Ces patients deviennent vulnérables aux infections et leur survie dépend de la régénération rapide de leur système hématopoïétique. Notre équipe a déjà mis au point une méthode pour préparer de grandes quantités des cellules mémoires capables de sécréter des IgG et des IgA. Les présents travaux visent à générer des plasmocytes fonctionnels et capables de survivre à long terme in vitro. La stratégie expérimentale visait à établir des conditions permettant de se rapprocher de l’environnement de la moelle osseuse. Dans un premier temps, nous avons étudié les paramètres permettant la différenciation des lymphocytes B mémoires en plasmocytes. Étant donné l’importance du potentiel redox dans l’environnement de la moelle osseuse, nous avons d’abord tenté d’en contrôler l’impact avec un antioxydant, le N-acétyle cystéine (NAC). Nos résultats ont démontré que le NAC avait un effet significatif et diminuait la phosphorylation de la protéine STAT3 en raison d’une inhibition des kinases JAK2 et JAK3. Étonnamment, cet antioxydant retardait la différenciation de nos lymphocytes B qui étaient stimulés avec une forte interaction CD40-CD154. Par la suite, la comparaison des interactions CD40-CD154 et CD27-CD70 a permis de conclure qu’il était essentiel de réduire à son minimum l’interaction CD40-CD154 et qu’il fallait ajouter les cytokines IL-6 et IL-10. Les cellules CD31+CD38+CD138+ générées présentaient un phénotype similaire à celui des plasmocytes de la moelle osseuse. Malheureusement la fréquence de ces cellules était faible et leur viabilité insuffisante. Afin d’augmenter la survie de ces cellules le dernier volet de nos travaux visait à se rapprocher des niches de la moelle osseuse. Notre but a été atteint en ajoutant des cellules mésenchymateuses issues de la moelle osseuse en présence de 8% de dioxygène (O2). Les cellules CD31+CD38+CD138+ générées ont une excellente viabilité et représentent plus de 50% des cellules totales en culture. De plus, le modèle de…

Advisors/Committee Members: Darveau, André, Néron, Sonia.

Subjects/Keywords: Plasmocytes; Reproduction; Lymphocytes B

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Bonnaure, G. (2016). Les plasmocytes et leur niche : étude de la génération de plasmocytes humains in vitro. (Thesis). Université Laval. Retrieved from http://hdl.handle.net/20.500.11794/27053

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Bonnaure, Guillaume. “Les plasmocytes et leur niche : étude de la génération de plasmocytes humains in vitro.” 2016. Thesis, Université Laval. Accessed January 20, 2021. http://hdl.handle.net/20.500.11794/27053.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Bonnaure, Guillaume. “Les plasmocytes et leur niche : étude de la génération de plasmocytes humains in vitro.” 2016. Web. 20 Jan 2021.

Vancouver:

Bonnaure G. Les plasmocytes et leur niche : étude de la génération de plasmocytes humains in vitro. [Internet] [Thesis]. Université Laval; 2016. [cited 2021 Jan 20]. Available from: http://hdl.handle.net/20.500.11794/27053.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Bonnaure G. Les plasmocytes et leur niche : étude de la génération de plasmocytes humains in vitro. [Thesis]. Université Laval; 2016. Available from: http://hdl.handle.net/20.500.11794/27053

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Université Laval

10. Martin, Guillaume. Impact fonctionnel de l'interaction kératinocyte-lymphocyte T au cours du psoriasis.

Degree: 2011, Université Laval

URL: http://hdl.handle.net/20.500.11794/22776

► Un important réseau de cytokines serait en grande partie responsable de la formation des lésions psoriasiques. La présence de cellules immunitaires dans des biopsies de… (more)

Subjects/Keywords: Psoriasis; Lymphocytes T; Kératinocytes

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Martin, G. (2011). Impact fonctionnel de l'interaction kératinocyte-lymphocyte T au cours du psoriasis. (Thesis). Université Laval. Retrieved from http://hdl.handle.net/20.500.11794/22776

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Martin, Guillaume. “Impact fonctionnel de l'interaction kératinocyte-lymphocyte T au cours du psoriasis.” 2011. Thesis, Université Laval. Accessed January 20, 2021. http://hdl.handle.net/20.500.11794/22776.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Martin, Guillaume. “Impact fonctionnel de l'interaction kératinocyte-lymphocyte T au cours du psoriasis.” 2011. Web. 20 Jan 2021.

Vancouver:

Martin G. Impact fonctionnel de l'interaction kératinocyte-lymphocyte T au cours du psoriasis. [Internet] [Thesis]. Université Laval; 2011. [cited 2021 Jan 20]. Available from: http://hdl.handle.net/20.500.11794/22776.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Martin G. Impact fonctionnel de l'interaction kératinocyte-lymphocyte T au cours du psoriasis. [Thesis]. Université Laval; 2011. Available from: http://hdl.handle.net/20.500.11794/22776

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Université Laval

11. Boucher-Lafleur, Anne-Marie. Étude des profils de méthylation et d'expression des lymphocytes T CD4+ dans l'asthme.

Degree: 2019, Université Laval

URL: http://hdl.handle.net/20.500.11794/37890

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Protocole d'entente entre l'Université Laval et l'Université du Québec à Chicoutimi

L’asthme est une maladie respiratoire chronique impliquant de l’inflammation, de l’hyperréactivité bronchique et un… (more)

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Protocole d'entente entre l'Université Laval et l'Université du Québec à Chicoutimi

L’asthme est une maladie respiratoire chronique impliquant de l’inflammation, de l’hyperréactivité bronchique et un remodelage des voies respiratoires. Il s’agit d’un trait complexe résultant d’interactions entre des facteurs génétiques et environnementaux. La portion génétique de l’asthme a été bien décrite, avec une héritabilité estimée jusqu’à 60% selon les études. Une portion de l’héritabilité manquante dans l’asthme pourrait être expliquée par des facteurs épigénétiques, comme la méthylation de l’ADN. Comme la méthylation est spécifique à chaque tissu, il est logique d’étudier des cellules ayant un rôle important dans l’asthme. C’est le cas des lymphocytes T CD4+ qui contribuent activement à la pathophysiologie de l’asthme. L’objectif du présent projet était donc d’étudier les lymphocytes T CD4+ dans un contexte d’asthme, plus particulièrement leur patron de méthylation ainsi que leur patron d’expression. Ces lymphocytes ont été isolés du sang de plus de 200 individus faisant partie de la Cohorte familiale d’asthme du Saguenay–Lac-Saint-Jean. La méthylation et l’expression a ensuite été mesurée par séquençage. Après des contrôles de qualité, des données étaient disponibles pour 173 individus (107 asthmatiques, 66 témoins). Il a ainsi été possible d’identifier 2 146 sites CpG différemment méthylés chez les asthmatiques et 3 756 chez les asthmatiques allergiques (FDR≤0,05, -meth ≥ 5%). Pour l’expression, 57 et 33 gènes sont différemment exprimés chez les asthmatiques et les asthmatiques allergiques, respectivement (FDR≤0,05). L’intégration des données génétique et d’expression ont permis d’identifier 2 253 eQTL significatifs (FDR≤0,049). Ces résultats ont permis d’identifier l’implication de la voie de NF-B ainsi que celle de l’IL-17 et -25 dans ces cellules, mais aussi l’implication de la famille de chemokine CXC et de EGF. En somme, cette étude a permis de caractériser plus précisément un type cellulaire dans l’asthme, toujours dans le but de mieux comprendre les mécanismes sous-jacents impliqués dans cette condition.

Advisors/Committee Members: Laprise, Catherine.

Subjects/Keywords: Lymphocytes T auxiliaires; Méthylation; Asthme

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Boucher-Lafleur, A. (2019). Étude des profils de méthylation et d'expression des lymphocytes T CD4+ dans l'asthme. (Thesis). Université Laval. Retrieved from http://hdl.handle.net/20.500.11794/37890

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Boucher-Lafleur, Anne-Marie. “Étude des profils de méthylation et d'expression des lymphocytes T CD4+ dans l'asthme.” 2019. Thesis, Université Laval. Accessed January 20, 2021. http://hdl.handle.net/20.500.11794/37890.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Boucher-Lafleur, Anne-Marie. “Étude des profils de méthylation et d'expression des lymphocytes T CD4+ dans l'asthme.” 2019. Web. 20 Jan 2021.

Vancouver:

Boucher-Lafleur A. Étude des profils de méthylation et d'expression des lymphocytes T CD4+ dans l'asthme. [Internet] [Thesis]. Université Laval; 2019. [cited 2021 Jan 20]. Available from: http://hdl.handle.net/20.500.11794/37890.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Boucher-Lafleur A. Étude des profils de méthylation et d'expression des lymphocytes T CD4+ dans l'asthme. [Thesis]. Université Laval; 2019. Available from: http://hdl.handle.net/20.500.11794/37890

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

12. Imbert, Caroline. Rôle de la sphingosine kinase-1 dans la réponse immunitaire anti-tumorale et dans le traitement du mélanome par immunothérapie : Role of sphingosine kinase-1 in the anti-tumor immune response and in the treatment of melanoma by immunotherapy.

Degree: Docteur es, Immunologie, 2018, Université Toulouse III – Paul Sabatier

URL: http://www.theses.fr/2018TOU30192

►

L'émergence de nouvelles approches thérapeutiques, basées sur des inhibiteurs de points de contrôle immunitaire (ICI), telles que les anti-CTLA-4 et anti-PD-1, ont révolutionné la prise… (more)

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L'émergence de nouvelles approches thérapeutiques, basées sur des inhibiteurs de points de contrôle immunitaire (ICI), telles que les anti-CTLA-4 et anti-PD-1, ont révolutionné la prise en charge du mélanome. Cependant les taux de réponses objectives à ces traitements restent relativement faibles et de sévères effets indésirables ou des résistances peuvent survenir. Il est donc nécessaire de comprendre les mécanismes de résistance et d'identifier des biomarqueurs prédictifs de la réponse aux ICI afin d'adapter la stratégie thérapeutique. Notre groupe a identifié des perturbations du métabolisme de la sphingosine 1-phosphate (S1P) dans les cellules de mélanome. Notamment, la surexpression de la Sphingosine Kinase-1 (SK1), une enzyme de production de la S1P qui favorise la progression tumorale en induisant des modifications phénotypiques du microenvironnement tumoral (TME), telles que la stromagenèse, l'angiogenèse et la polarisation M2 des macrophages. Or, la S1P joue un rôle important dans l'immunité car elle régule de nombreux processus comme l'activation, la différenciation et le trafic de nombreuses cellules immunitaires. L'objectif de cette thèse a été de déterminer si l'inhibition de la SK1 affecte la réponse immunitaire anti-tumorale et améliore l'efficacité des ICI. Nous avons mis en évidence que l'expression accrue de SK1 dans les cellules tumorales est significativement associée à une survie plus courte chez les patients atteints de mélanome métastatique traités par anti-PD-1. Ensuite, nous avons démontré, dans un modèle murin, que la diminution de l'expression de SK1 dans la tumeur induit une réduction de la croissance du mélanome qui est associée à une diminution de l'expression de diverses molécules immunosuppressives dans le TME. Cette modification du TME diminue la prolifération mais aussi l'infiltration des lymphocytes T régulateurs (Treg) et par conséquent induit une augmentation du ratio CD8+/Treg, ce qui est associé à un bon pronostic. Une signature immunosuppressive dépendante de SK1 a également été observée dans des biopsies de mélanome humain. De manière importante, nos données révèlent que l'inhibition de SK1 améliore fortement l'efficacité des ICI, tels que les anti-CTLA-4 ou les anti-PD-1, conduisant au rejet des tumeurs et à une amélioration significative de la survie des animaux dans un modèle murin de mélanome. Des résultats significatifs ont également été obtenus dans des modèles de cancer du sein et du côlon. Ici, nous montrons que: i) le ciblage de SK1 dans les mélanomes conduit à une augmentation du rapport intratumoral CD8+/Treg, ii) la combinaison de l'inhibition de SK1 avec des ICI améliore grandement la survie des souris et iii) la SK1 pourrait constituer un nouveau biomarqueur prédictif de la réponse aux ICI chez l'Homme. Par conséquent, nos données ont identifié la SK1 comme une kinase lipidique agissant comme un nouveau point de contrôle immunitaire, qui pourrait être ciblée afin d'améliorer la réponse aux ICI dans différents cancers.

The emergence of new therapeutic…

Advisors/Committee Members: Andrieu-Abadie, Nathalie (thesis director), Colacios, Céline (thesis director).

Subjects/Keywords: Immunothérapies; Mélanome; Sphingolipides; Lymphocytes; Biomarqueur

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Imbert, C. (2018). Rôle de la sphingosine kinase-1 dans la réponse immunitaire anti-tumorale et dans le traitement du mélanome par immunothérapie : Role of sphingosine kinase-1 in the anti-tumor immune response and in the treatment of melanoma by immunotherapy. (Doctoral Dissertation). Université Toulouse III – Paul Sabatier. Retrieved from http://www.theses.fr/2018TOU30192

Chicago Manual of Style (16^th Edition):

Imbert, Caroline. “Rôle de la sphingosine kinase-1 dans la réponse immunitaire anti-tumorale et dans le traitement du mélanome par immunothérapie : Role of sphingosine kinase-1 in the anti-tumor immune response and in the treatment of melanoma by immunotherapy.” 2018. Doctoral Dissertation, Université Toulouse III – Paul Sabatier. Accessed January 20, 2021. http://www.theses.fr/2018TOU30192.

MLA Handbook (7^th Edition):

Imbert, Caroline. “Rôle de la sphingosine kinase-1 dans la réponse immunitaire anti-tumorale et dans le traitement du mélanome par immunothérapie : Role of sphingosine kinase-1 in the anti-tumor immune response and in the treatment of melanoma by immunotherapy.” 2018. Web. 20 Jan 2021.

Vancouver:

Imbert C. Rôle de la sphingosine kinase-1 dans la réponse immunitaire anti-tumorale et dans le traitement du mélanome par immunothérapie : Role of sphingosine kinase-1 in the anti-tumor immune response and in the treatment of melanoma by immunotherapy. [Internet] [Doctoral dissertation]. Université Toulouse III – Paul Sabatier; 2018. [cited 2021 Jan 20]. Available from: http://www.theses.fr/2018TOU30192.

Council of Science Editors:

Imbert C. Rôle de la sphingosine kinase-1 dans la réponse immunitaire anti-tumorale et dans le traitement du mélanome par immunothérapie : Role of sphingosine kinase-1 in the anti-tumor immune response and in the treatment of melanoma by immunotherapy. [Doctoral Dissertation]. Université Toulouse III – Paul Sabatier; 2018. Available from: http://www.theses.fr/2018TOU30192

Hong Kong University of Science and Technology

13. Ye, Mulin. Initial study of the role of native interleukin-15 in generating tumor-specific cytotoxic T lymphocytes in a dendritic cell-mediated ex vivo system.

Degree: 2011, Hong Kong University of Science and Technology

URL: http://repository.ust.hk/ir/Record/1783.1-7915 ; https://doi.org/10.14711/thesis-b1155457 ; http://repository.ust.hk/ir/bitstream/1783.1-7915/1/th_redirect.html

► Adoptive cell transfer (ACT) immunotherapy using ex vivo expanded tumor-specificcytotoxic T lymphocytes (CTLs) is one of the most promising approaches for treating cancers. The quality… (more)

Subjects/Keywords: Lymphocytes ; Interleukins ; Cellular therapy

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Ye, M. (2011). Initial study of the role of native interleukin-15 in generating tumor-specific cytotoxic T lymphocytes in a dendritic cell-mediated ex vivo system. (Thesis). Hong Kong University of Science and Technology. Retrieved from http://repository.ust.hk/ir/Record/1783.1-7915 ; https://doi.org/10.14711/thesis-b1155457 ; http://repository.ust.hk/ir/bitstream/1783.1-7915/1/th_redirect.html

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Ye, Mulin. “Initial study of the role of native interleukin-15 in generating tumor-specific cytotoxic T lymphocytes in a dendritic cell-mediated ex vivo system.” 2011. Thesis, Hong Kong University of Science and Technology. Accessed January 20, 2021. http://repository.ust.hk/ir/Record/1783.1-7915 ; https://doi.org/10.14711/thesis-b1155457 ; http://repository.ust.hk/ir/bitstream/1783.1-7915/1/th_redirect.html.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Ye, Mulin. “Initial study of the role of native interleukin-15 in generating tumor-specific cytotoxic T lymphocytes in a dendritic cell-mediated ex vivo system.” 2011. Web. 20 Jan 2021.

Vancouver:

Ye M. Initial study of the role of native interleukin-15 in generating tumor-specific cytotoxic T lymphocytes in a dendritic cell-mediated ex vivo system. [Internet] [Thesis]. Hong Kong University of Science and Technology; 2011. [cited 2021 Jan 20]. Available from: http://repository.ust.hk/ir/Record/1783.1-7915 ; https://doi.org/10.14711/thesis-b1155457 ; http://repository.ust.hk/ir/bitstream/1783.1-7915/1/th_redirect.html.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Ye M. Initial study of the role of native interleukin-15 in generating tumor-specific cytotoxic T lymphocytes in a dendritic cell-mediated ex vivo system. [Thesis]. Hong Kong University of Science and Technology; 2011. Available from: http://repository.ust.hk/ir/Record/1783.1-7915 ; https://doi.org/10.14711/thesis-b1155457 ; http://repository.ust.hk/ir/bitstream/1783.1-7915/1/th_redirect.html

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

14. Rozing, Jan. B lymphocyte differentiation in the mouse.

Degree: 1977, Erasmus University Medical Center

URL: http://hdl.handle.net/1765/26067

► textabstractWhen an antigen enters the body, it can react upon such an invasion with a nonspecific and a specific defense mechardsm. Phagocytic white blood cells… (more)

Subjects/Keywords: antigen; lymphocytes

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Rozing, J. (1977). B lymphocyte differentiation in the mouse. (Doctoral Dissertation). Erasmus University Medical Center. Retrieved from http://hdl.handle.net/1765/26067

Chicago Manual of Style (16^th Edition):

Rozing, Jan. “B lymphocyte differentiation in the mouse.” 1977. Doctoral Dissertation, Erasmus University Medical Center. Accessed January 20, 2021. http://hdl.handle.net/1765/26067.

MLA Handbook (7^th Edition):

Rozing, Jan. “B lymphocyte differentiation in the mouse.” 1977. Web. 20 Jan 2021.

Vancouver:

Rozing J. B lymphocyte differentiation in the mouse. [Internet] [Doctoral dissertation]. Erasmus University Medical Center; 1977. [cited 2021 Jan 20]. Available from: http://hdl.handle.net/1765/26067.

Council of Science Editors:

Rozing J. B lymphocyte differentiation in the mouse. [Doctoral Dissertation]. Erasmus University Medical Center; 1977. Available from: http://hdl.handle.net/1765/26067

Université de Sherbrooke

15. Fock-Chow-Tho, David. Développement de stratégies et d'outils analytiques novateurs pour la détection de vaches atteintes de paratuberculose.

Degree: 2016, Université de Sherbrooke

URL: http://hdl.handle.net/11143/9774

► La paratuberculose bovine est une maladie causée par la mycobactérie Mycobacterium avium subsp. paratuberculosis (MAP). Elle est responsable d’énormes pertes économiques dans le monde. En… (more)

▼ La paratuberculose bovine est une maladie causée par la mycobactérie Mycobacterium avium subsp. paratuberculosis (MAP). Elle est responsable d’énormes pertes économiques dans le monde. En effet, cette maladie provoque la diminution de la production laitière chez les vaches ainsi qu’un état de fatigue général en raison d’une entérite chronique chez les sujets atteints. Des indices mettent également en évidence le potentiel zoonotique de MAP et malheureusement aucun traitement n’est connu à ce jour. Ainsi, les stratégies actuelles pour contrer la maladie dans les troupeaux reposent plutôt sur des actions préventives. Actuellement, le diagnostic de la paratuberculose reste difficile et la prévalence de la maladie est probablement sous-estimée dans les troupeaux en raison de la faible sensibilité des tests diagnostiques. Dans l’optique de développer une nouvelle méthode diagnostique pour la paratuberculose, ce projet de recherche s’est établi en deux étapes : la mise en place d’un système de diagnostic robuste des animaux par les méthodes de dépistage traditionnelles, incluant une étude comparative de l’efficacité de trousses commerciales, et finalement l’étude de la prolifération cellulaire spécifique des lymphocytes comme d’une épreuve diagnostique pour la maladie. Ainsi, la comparaison des trousses commerciales a démontré un écart d’efficacité qui a permis d’établir de nouvelles recommandations pour l’analyse des animaux ainsi que l’amélioration du diagnostic de la paratuberculose dans les troupeaux. Concernant le développement d’une méthode de diagnostique basée sur la prolifération lymphocytaire, de nombreuses difficultés techniques ont entravé le bon déroulement du projet mais ces travaux montrent la possibilité de caractériser de manière plus complète les différents types lymphocytaires par cytométrie de flux chez le bovin laitier. Advisors/Committee Members: Bissonnette, Nathalie (advisor), Steimle, Viktor (advisor).

Subjects/Keywords: Paratuberculose bovine; Lymphocytes; Prolifération; Diagnostic

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APA (6^th Edition):

Fock-Chow-Tho, D. (2016). Développement de stratégies et d'outils analytiques novateurs pour la détection de vaches atteintes de paratuberculose. (Masters Thesis). Université de Sherbrooke. Retrieved from http://hdl.handle.net/11143/9774

Chicago Manual of Style (16^th Edition):

Fock-Chow-Tho, David. “Développement de stratégies et d'outils analytiques novateurs pour la détection de vaches atteintes de paratuberculose.” 2016. Masters Thesis, Université de Sherbrooke. Accessed January 20, 2021. http://hdl.handle.net/11143/9774.

MLA Handbook (7^th Edition):

Fock-Chow-Tho, David. “Développement de stratégies et d'outils analytiques novateurs pour la détection de vaches atteintes de paratuberculose.” 2016. Web. 20 Jan 2021.

Vancouver:

Fock-Chow-Tho D. Développement de stratégies et d'outils analytiques novateurs pour la détection de vaches atteintes de paratuberculose. [Internet] [Masters thesis]. Université de Sherbrooke; 2016. [cited 2021 Jan 20]. Available from: http://hdl.handle.net/11143/9774.

Council of Science Editors:

Fock-Chow-Tho D. Développement de stratégies et d'outils analytiques novateurs pour la détection de vaches atteintes de paratuberculose. [Masters Thesis]. Université de Sherbrooke; 2016. Available from: http://hdl.handle.net/11143/9774

University of Toronto

16. Guerra, Fiona. The Role of Homeodomain-interacting Protein Kinase (HIPK)-1 in B Lymphocytes.

Degree: 2011, University of Toronto

URL: http://hdl.handle.net/1807/29736

►

The homeodomain-interacting protein kinase (HIPK) family is comprised of four evolutionarily conserved and highly related serine/threonine kinases originally identified as co-repressors for homeodomain-containing transcription factors.… (more)

Subjects/Keywords: immunology; B lymphocytes; 0982

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APA (6^th Edition):

Guerra, F. (2011). The Role of Homeodomain-interacting Protein Kinase (HIPK)-1 in B Lymphocytes. (Doctoral Dissertation). University of Toronto. Retrieved from http://hdl.handle.net/1807/29736

Chicago Manual of Style (16^th Edition):

Guerra, Fiona. “The Role of Homeodomain-interacting Protein Kinase (HIPK)-1 in B Lymphocytes.” 2011. Doctoral Dissertation, University of Toronto. Accessed January 20, 2021. http://hdl.handle.net/1807/29736.

MLA Handbook (7^th Edition):

Guerra, Fiona. “The Role of Homeodomain-interacting Protein Kinase (HIPK)-1 in B Lymphocytes.” 2011. Web. 20 Jan 2021.

Vancouver:

Guerra F. The Role of Homeodomain-interacting Protein Kinase (HIPK)-1 in B Lymphocytes. [Internet] [Doctoral dissertation]. University of Toronto; 2011. [cited 2021 Jan 20]. Available from: http://hdl.handle.net/1807/29736.

Council of Science Editors:

Guerra F. The Role of Homeodomain-interacting Protein Kinase (HIPK)-1 in B Lymphocytes. [Doctoral Dissertation]. University of Toronto; 2011. Available from: http://hdl.handle.net/1807/29736

University of Arizona

17. Kiehn, Timothy Everett, 1939-. COMPUTER ANALYSIS OF TRANSFORMING LYMPHOCYTES .

Degree: 1972, University of Arizona

URL: http://hdl.handle.net/10150/287841

Subjects/Keywords: Lymphocytes – Research.

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APA (6^th Edition):

Kiehn, Timothy Everett, 1. (1972). COMPUTER ANALYSIS OF TRANSFORMING LYMPHOCYTES . (Doctoral Dissertation). University of Arizona. Retrieved from http://hdl.handle.net/10150/287841

Chicago Manual of Style (16^th Edition):

Kiehn, Timothy Everett, 1939-. “COMPUTER ANALYSIS OF TRANSFORMING LYMPHOCYTES .” 1972. Doctoral Dissertation, University of Arizona. Accessed January 20, 2021. http://hdl.handle.net/10150/287841.

MLA Handbook (7^th Edition):

Kiehn, Timothy Everett, 1939-. “COMPUTER ANALYSIS OF TRANSFORMING LYMPHOCYTES .” 1972. Web. 20 Jan 2021.

Vancouver:

Kiehn, Timothy Everett 1. COMPUTER ANALYSIS OF TRANSFORMING LYMPHOCYTES . [Internet] [Doctoral dissertation]. University of Arizona; 1972. [cited 2021 Jan 20]. Available from: http://hdl.handle.net/10150/287841.

Council of Science Editors:

Kiehn, Timothy Everett 1. COMPUTER ANALYSIS OF TRANSFORMING LYMPHOCYTES . [Doctoral Dissertation]. University of Arizona; 1972. Available from: http://hdl.handle.net/10150/287841

University of Arizona

18. Dean, Jack H. STUDIES ON THE INTERACTION OF LYMPHOCYTES WITH POLYRIBOINOSINIC: POLYRIBOCYTIDYLIC ACID; LYMPHOCYTE ACTIVATION AND INTERFERON PRODUCTION .

Degree: 1972, University of Arizona

URL: http://hdl.handle.net/10150/287998

Subjects/Keywords: Interferon.; Lymphocytes.

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APA (6^th Edition):

Dean, J. H. (1972). STUDIES ON THE INTERACTION OF LYMPHOCYTES WITH POLYRIBOINOSINIC: POLYRIBOCYTIDYLIC ACID; LYMPHOCYTE ACTIVATION AND INTERFERON PRODUCTION . (Doctoral Dissertation). University of Arizona. Retrieved from http://hdl.handle.net/10150/287998

Chicago Manual of Style (16^th Edition):

Dean, Jack H. “STUDIES ON THE INTERACTION OF LYMPHOCYTES WITH POLYRIBOINOSINIC: POLYRIBOCYTIDYLIC ACID; LYMPHOCYTE ACTIVATION AND INTERFERON PRODUCTION .” 1972. Doctoral Dissertation, University of Arizona. Accessed January 20, 2021. http://hdl.handle.net/10150/287998.

MLA Handbook (7^th Edition):

Dean, Jack H. “STUDIES ON THE INTERACTION OF LYMPHOCYTES WITH POLYRIBOINOSINIC: POLYRIBOCYTIDYLIC ACID; LYMPHOCYTE ACTIVATION AND INTERFERON PRODUCTION .” 1972. Web. 20 Jan 2021.

Vancouver:

Dean JH. STUDIES ON THE INTERACTION OF LYMPHOCYTES WITH POLYRIBOINOSINIC: POLYRIBOCYTIDYLIC ACID; LYMPHOCYTE ACTIVATION AND INTERFERON PRODUCTION . [Internet] [Doctoral dissertation]. University of Arizona; 1972. [cited 2021 Jan 20]. Available from: http://hdl.handle.net/10150/287998.

Council of Science Editors:

Dean JH. STUDIES ON THE INTERACTION OF LYMPHOCYTES WITH POLYRIBOINOSINIC: POLYRIBOCYTIDYLIC ACID; LYMPHOCYTE ACTIVATION AND INTERFERON PRODUCTION . [Doctoral Dissertation]. University of Arizona; 1972. Available from: http://hdl.handle.net/10150/287998

University of Arizona

19. Endres, Robert Otto, 1949-. INVESTIGATION OF THE ROLE OF LYMPHOCYTE ECTOGLYCOSYLTRANSFERASES IN BLASTOGENESIS AND CELLULAR INTERACTIONS .

Degree: 1976, University of Arizona

URL: http://hdl.handle.net/10150/289413

Subjects/Keywords: Lymphocytes.; Ectoglycosyltransferase.

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APA (6^th Edition):

Endres, Robert Otto, 1. (1976). INVESTIGATION OF THE ROLE OF LYMPHOCYTE ECTOGLYCOSYLTRANSFERASES IN BLASTOGENESIS AND CELLULAR INTERACTIONS . (Doctoral Dissertation). University of Arizona. Retrieved from http://hdl.handle.net/10150/289413

Chicago Manual of Style (16^th Edition):

Endres, Robert Otto, 1949-. “INVESTIGATION OF THE ROLE OF LYMPHOCYTE ECTOGLYCOSYLTRANSFERASES IN BLASTOGENESIS AND CELLULAR INTERACTIONS .” 1976. Doctoral Dissertation, University of Arizona. Accessed January 20, 2021. http://hdl.handle.net/10150/289413.

MLA Handbook (7^th Edition):

Endres, Robert Otto, 1949-. “INVESTIGATION OF THE ROLE OF LYMPHOCYTE ECTOGLYCOSYLTRANSFERASES IN BLASTOGENESIS AND CELLULAR INTERACTIONS .” 1976. Web. 20 Jan 2021.

Vancouver:

Endres, Robert Otto 1. INVESTIGATION OF THE ROLE OF LYMPHOCYTE ECTOGLYCOSYLTRANSFERASES IN BLASTOGENESIS AND CELLULAR INTERACTIONS . [Internet] [Doctoral dissertation]. University of Arizona; 1976. [cited 2021 Jan 20]. Available from: http://hdl.handle.net/10150/289413.

Council of Science Editors:

Endres, Robert Otto 1. INVESTIGATION OF THE ROLE OF LYMPHOCYTE ECTOGLYCOSYLTRANSFERASES IN BLASTOGENESIS AND CELLULAR INTERACTIONS . [Doctoral Dissertation]. University of Arizona; 1976. Available from: http://hdl.handle.net/10150/289413

Queens University

20. Seaward, Alexandra Victoria Catherine. Effects of Diabetes on Lymphocyte Phenotype and Function During Pregnancy .

Degree: Anatomy and Cell Biology, 2011, Queens University

URL: http://hdl.handle.net/1974/6299

► Diabetic pregnant women have an increased risk of developing pre-eclampsia, a late gestational syndrome, although the reason for this gain is unknown. Placental pathology in… (more)

▼ Diabetic pregnant women have an increased risk of developing pre-eclampsia, a late gestational syndrome, although the reason for this gain is unknown. Placental pathology in pre-eclampsia is linked with insufficient spiral arterial modification, a process triggered by uterine natural killer (uNK) cells, an abundant pro-angiogenic cell type found in early pregnancy decidua (endometrium of the pregnant uterus). UNK cells effect spiral arterial modification, the blood supply to the placenta, through the release of interferon gamma (Ifng), a pro-inflammatory cytokine. Peripheral blood precursors of uNK cells employ a unique pattern of homing molecules to traffic to the decidua. The goal of this thesis was to advance the understanding of how homing and functions of uNK cell precursors might be modified in diabetic pregnancy. Studies employed both murine and human models. Pregnancies were studied microscopically in normoglycemic (n-) and hyperglycemic (d-) non-obese diabetic (NOD) and NOD.Ifng-/- (NOD strain with a genetic deletion of Ifng) mice. Pre-implantation embryo development was impaired in n- and d-NOD.Ifng-/- mice. Hyperglycemia decreased both numbers of uNK cells and spiral artery remodelling within d-NOD and d-NOD.Ifng-/- decidua. This decrease in spiral artery remodelling was independent of Ifng and linked with hypertrophy of smooth muscle in implantation sites. Blood leukocytes from control and diabetic pregnant women were evaluated for adhesive function and expression of key homing molecules. Diabetic leukocytes had decreased CD56+ (uNK cell lineage) cell adhesion to decidua, increased CD56+ cell adhesion to pancreas, and comparable adhesion to lymph node compared with control leukocytes, suggesting impaired decidual homing in vivo. Of 8 lymphocyte subsets resolved by flow cytometry, type 1 cytokine CD56bright cells expressed appropriate homing molecules most abundantly. Diabetes did not alter levels of expression of these receptors. These data show that diabetes alters the potential capacity for decidual homing of pre-uNK cells but that this is not achieved through reduction in levels of key homing molecules. Diabetes also reduced spiral arterial modification in mice through hypertrophy of smooth muscle. The reproductive challenges of diabetic women who have co-morbid immunological diseases merit further study.

Subjects/Keywords: Diabetes ; Immunology ; Lymphocytes ; Pregnancy

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APA (6^th Edition):

Seaward, A. V. C. (2011). Effects of Diabetes on Lymphocyte Phenotype and Function During Pregnancy . (Thesis). Queens University. Retrieved from http://hdl.handle.net/1974/6299

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Seaward, Alexandra Victoria Catherine. “Effects of Diabetes on Lymphocyte Phenotype and Function During Pregnancy .” 2011. Thesis, Queens University. Accessed January 20, 2021. http://hdl.handle.net/1974/6299.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Seaward, Alexandra Victoria Catherine. “Effects of Diabetes on Lymphocyte Phenotype and Function During Pregnancy .” 2011. Web. 20 Jan 2021.

Vancouver:

Seaward AVC. Effects of Diabetes on Lymphocyte Phenotype and Function During Pregnancy . [Internet] [Thesis]. Queens University; 2011. [cited 2021 Jan 20]. Available from: http://hdl.handle.net/1974/6299.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Seaward AVC. Effects of Diabetes on Lymphocyte Phenotype and Function During Pregnancy . [Thesis]. Queens University; 2011. Available from: http://hdl.handle.net/1974/6299

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Georgia State University

21. Murali, Vineeth Kumar. Adenovirus Death Protein: The Switch Between Lytic and Persistent Infections in Lymphocytes?.

Degree: MS, Biology, 2012, Georgia State University

URL: https://scholarworks.gsu.edu/biology_theses/41

► ABSTRACT Adenovirus Death Protein (ADP) expression during late stages of a lytic infection releases mature virions to promote viral spread, thus leading to death… (more)

Subjects/Keywords: Adenovirus; ADP; Persistent infection; Lymphocytes

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APA (6^th Edition):

Murali, V. K. (2012). Adenovirus Death Protein: The Switch Between Lytic and Persistent Infections in Lymphocytes?. (Thesis). Georgia State University. Retrieved from https://scholarworks.gsu.edu/biology_theses/41

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Murali, Vineeth Kumar. “Adenovirus Death Protein: The Switch Between Lytic and Persistent Infections in Lymphocytes?.” 2012. Thesis, Georgia State University. Accessed January 20, 2021. https://scholarworks.gsu.edu/biology_theses/41.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Murali, Vineeth Kumar. “Adenovirus Death Protein: The Switch Between Lytic and Persistent Infections in Lymphocytes?.” 2012. Web. 20 Jan 2021.

Vancouver:

Murali VK. Adenovirus Death Protein: The Switch Between Lytic and Persistent Infections in Lymphocytes?. [Internet] [Thesis]. Georgia State University; 2012. [cited 2021 Jan 20]. Available from: https://scholarworks.gsu.edu/biology_theses/41.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Murali VK. Adenovirus Death Protein: The Switch Between Lytic and Persistent Infections in Lymphocytes?. [Thesis]. Georgia State University; 2012. Available from: https://scholarworks.gsu.edu/biology_theses/41

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of British Columbia

22. Wong, S. Y. Comparison between locomotory behavior of lymphocytes of WWV mutant and normal house-mouse.

Degree: MS- MSc, Zoology, 1969, University of British Columbia

URL: http://hdl.handle.net/2429/35299

► The rate of motility of lymphocytes from anemic mice of the WW[superscript V] genotype and their normal ++ littermates was determined in vitro by time-lapse… (more)

Subjects/Keywords: Lymphocytes; Mice

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APA (6^th Edition):

Wong, S. Y. (1969). Comparison between locomotory behavior of lymphocytes of WWV mutant and normal house-mouse. (Masters Thesis). University of British Columbia. Retrieved from http://hdl.handle.net/2429/35299

Chicago Manual of Style (16^th Edition):

Wong, S Y. “Comparison between locomotory behavior of lymphocytes of WWV mutant and normal house-mouse.” 1969. Masters Thesis, University of British Columbia. Accessed January 20, 2021. http://hdl.handle.net/2429/35299.

MLA Handbook (7^th Edition):

Wong, S Y. “Comparison between locomotory behavior of lymphocytes of WWV mutant and normal house-mouse.” 1969. Web. 20 Jan 2021.

Vancouver:

Wong SY. Comparison between locomotory behavior of lymphocytes of WWV mutant and normal house-mouse. [Internet] [Masters thesis]. University of British Columbia; 1969. [cited 2021 Jan 20]. Available from: http://hdl.handle.net/2429/35299.

Council of Science Editors:

Wong SY. Comparison between locomotory behavior of lymphocytes of WWV mutant and normal house-mouse. [Masters Thesis]. University of British Columbia; 1969. Available from: http://hdl.handle.net/2429/35299

The Ohio State University

23. Cannon, Marvin Samuel. A morphological cytochemical and statistical approach to the classification of lymphocytes.

Degree: PhD, Graduate School, 1969, The Ohio State University

URL: http://rave.ohiolink.edu/etdc/view?acc_num=osu1486654338997873

Subjects/Keywords: Biology; Lymphocytes

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APA (6^th Edition):

Cannon, M. S. (1969). A morphological cytochemical and statistical approach to the classification of lymphocytes. (Doctoral Dissertation). The Ohio State University. Retrieved from http://rave.ohiolink.edu/etdc/view?acc_num=osu1486654338997873

Chicago Manual of Style (16^th Edition):

Cannon, Marvin Samuel. “A morphological cytochemical and statistical approach to the classification of lymphocytes.” 1969. Doctoral Dissertation, The Ohio State University. Accessed January 20, 2021. http://rave.ohiolink.edu/etdc/view?acc_num=osu1486654338997873.

MLA Handbook (7^th Edition):

Cannon, Marvin Samuel. “A morphological cytochemical and statistical approach to the classification of lymphocytes.” 1969. Web. 20 Jan 2021.

Vancouver:

Cannon MS. A morphological cytochemical and statistical approach to the classification of lymphocytes. [Internet] [Doctoral dissertation]. The Ohio State University; 1969. [cited 2021 Jan 20]. Available from: http://rave.ohiolink.edu/etdc/view?acc_num=osu1486654338997873.

Council of Science Editors:

Cannon MS. A morphological cytochemical and statistical approach to the classification of lymphocytes. [Doctoral Dissertation]. The Ohio State University; 1969. Available from: http://rave.ohiolink.edu/etdc/view?acc_num=osu1486654338997873

The Ohio State University

24. Waksal, Samuel David. In vitro studies on thymus derived lymphocytes : differentiation of T-lymphocytes and their function in tumor destruction.

Degree: PhD, Graduate School, 1974, The Ohio State University

URL: http://rave.ohiolink.edu/etdc/view?acc_num=osu1486990930833541

Subjects/Keywords: Biology; Lymphocytes

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APA (6^th Edition):

Waksal, S. D. (1974). In vitro studies on thymus derived lymphocytes : differentiation of T-lymphocytes and their function in tumor destruction. (Doctoral Dissertation). The Ohio State University. Retrieved from http://rave.ohiolink.edu/etdc/view?acc_num=osu1486990930833541

Chicago Manual of Style (16^th Edition):

Waksal, Samuel David. “In vitro studies on thymus derived lymphocytes : differentiation of T-lymphocytes and their function in tumor destruction.” 1974. Doctoral Dissertation, The Ohio State University. Accessed January 20, 2021. http://rave.ohiolink.edu/etdc/view?acc_num=osu1486990930833541.

MLA Handbook (7^th Edition):

Waksal, Samuel David. “In vitro studies on thymus derived lymphocytes : differentiation of T-lymphocytes and their function in tumor destruction.” 1974. Web. 20 Jan 2021.

Vancouver:

Waksal SD. In vitro studies on thymus derived lymphocytes : differentiation of T-lymphocytes and their function in tumor destruction. [Internet] [Doctoral dissertation]. The Ohio State University; 1974. [cited 2021 Jan 20]. Available from: http://rave.ohiolink.edu/etdc/view?acc_num=osu1486990930833541.

Council of Science Editors:

Waksal SD. In vitro studies on thymus derived lymphocytes : differentiation of T-lymphocytes and their function in tumor destruction. [Doctoral Dissertation]. The Ohio State University; 1974. Available from: http://rave.ohiolink.edu/etdc/view?acc_num=osu1486990930833541

Michigan State University

25. Chang, Hsun-Lang. The developmental expression of CD45 glycoprotein in murine t lymphocytes.

Degree: PhD, Department of Microbiology, 1990, Michigan State University

URL: http://etd.lib.msu.edu/islandora/object/etd:29092

Subjects/Keywords: Glycoproteins; Lymphocytes

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APA (6^th Edition):

Chang, H. (1990). The developmental expression of CD45 glycoprotein in murine t lymphocytes. (Doctoral Dissertation). Michigan State University. Retrieved from http://etd.lib.msu.edu/islandora/object/etd:29092

Chicago Manual of Style (16^th Edition):

Chang, Hsun-Lang. “The developmental expression of CD45 glycoprotein in murine t lymphocytes.” 1990. Doctoral Dissertation, Michigan State University. Accessed January 20, 2021. http://etd.lib.msu.edu/islandora/object/etd:29092.

MLA Handbook (7^th Edition):

Chang, Hsun-Lang. “The developmental expression of CD45 glycoprotein in murine t lymphocytes.” 1990. Web. 20 Jan 2021.

Vancouver:

Chang H. The developmental expression of CD45 glycoprotein in murine t lymphocytes. [Internet] [Doctoral dissertation]. Michigan State University; 1990. [cited 2021 Jan 20]. Available from: http://etd.lib.msu.edu/islandora/object/etd:29092.

Council of Science Editors:

Chang H. The developmental expression of CD45 glycoprotein in murine t lymphocytes. [Doctoral Dissertation]. Michigan State University; 1990. Available from: http://etd.lib.msu.edu/islandora/object/etd:29092

26. Benbarche, Salima. Influence des cytokines immunitaires sur la mégacaryocytopoïèse et l'homéostasie des plaquettes sanguines : rôle de l'interleukine 21 : Effect of immune cytokines on megakaryocytopoiesis and platelet homeostasis : role of interleukin 21.

Degree: Docteur es, Sciences de la vie et de la santé, 2015, Université de Strasbourg

URL: http://www.theses.fr/2015STRAJ044

►

La mégacaryocytopoïèse est le processus de différenciation des cellules souches hématopoïétiques en mégacaryocytes produisant les plaquettes sanguines. Des données de transcriptome indiquent la présence des… (more)

▼

La mégacaryocytopoïèse est le processus de différenciation des cellules souches hématopoïétiques en mégacaryocytes produisant les plaquettes sanguines. Des données de transcriptome indiquent la présence des récepteurs aux interleukines (IL) -10, -17A et -21 sur les mégacaryocytes. Mon travail montre que les IL-10 et -17 n’ont pas d’effet apparent sur la prolifération et la différenciation in vitro des mégacaryocytes à partir des progéniteurs hématopoïétiques humains CD34+. Cependant, l’IL-21 augmente la prolifération des progéniteurs mégacaryocytaires dérivés de ces cellules et sans modifier leur différenciation. Les mégacaryocytes de la moelle osseuse humaine expriment aussi le récepteur à l’IL-21, ce qui suggère un rôle direct de l’IL-21 sur la mégacaryocytopoïèse in vivo. De façon concordante, l’expression de l’IL-21 chez la souris stimule la mégacaryocytopoïèse ainsi que la production des plaquettes, mais augmente la clairance des plaquettes par les macrophages. Ces travaux suggèrent que durant les réponses immunitaires, l’expression de l’IL-21 par les lymphocytes T CD4+ activés module l’homéostasie des plaquettes sanguines.

Megakaryocytopoiesis is the process by which hematopoietic stem cells give rise to megakaryocytes which in turn produce blood platelets. Transcriptional studies indicate the presence of interleukin (IL) -10, -17 and -21 receptors on megakaryocytes. This work shows that IL-10 and -17 have no apparent effect on in vitro proliferation and differentiation of megakaryocytes derived from human CD34+ hematopoietic progenitors. However, IL-21 increases the proliferation of megakaryocyte progenitors derived from these CD34+ cells without modifying their differentiation. Moreover, human bone marrow megakaryocytes express IL-21 receptor, suggesting a direct role of IL-21 on megakaryocytopoiesis in vivo. Concordantly, IL-21 expression in mice stimulates megakaryocytopoiesis and platelet production, but increases platelet clearance by macrophages. This work suggest that during immune responses, the expression of IL-21 by activated CD4+ T lymphocytes modulate blood platelet homeostasis.

Advisors/Committee Members: De la Salle, Henri (thesis director).

Subjects/Keywords: Mégacaryocytopoïèse; Plaquettes; IL-21; Lymphocytes; Megakaryocytopoiesis; Platelets; IL-21; Lymphocytes; 573.1

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APA (6^th Edition):

Benbarche, S. (2015). Influence des cytokines immunitaires sur la mégacaryocytopoïèse et l'homéostasie des plaquettes sanguines : rôle de l'interleukine 21 : Effect of immune cytokines on megakaryocytopoiesis and platelet homeostasis : role of interleukin 21. (Doctoral Dissertation). Université de Strasbourg. Retrieved from http://www.theses.fr/2015STRAJ044

Chicago Manual of Style (16^th Edition):

Benbarche, Salima. “Influence des cytokines immunitaires sur la mégacaryocytopoïèse et l'homéostasie des plaquettes sanguines : rôle de l'interleukine 21 : Effect of immune cytokines on megakaryocytopoiesis and platelet homeostasis : role of interleukin 21.” 2015. Doctoral Dissertation, Université de Strasbourg. Accessed January 20, 2021. http://www.theses.fr/2015STRAJ044.

MLA Handbook (7^th Edition):

Benbarche, Salima. “Influence des cytokines immunitaires sur la mégacaryocytopoïèse et l'homéostasie des plaquettes sanguines : rôle de l'interleukine 21 : Effect of immune cytokines on megakaryocytopoiesis and platelet homeostasis : role of interleukin 21.” 2015. Web. 20 Jan 2021.

Vancouver:

Benbarche S. Influence des cytokines immunitaires sur la mégacaryocytopoïèse et l'homéostasie des plaquettes sanguines : rôle de l'interleukine 21 : Effect of immune cytokines on megakaryocytopoiesis and platelet homeostasis : role of interleukin 21. [Internet] [Doctoral dissertation]. Université de Strasbourg; 2015. [cited 2021 Jan 20]. Available from: http://www.theses.fr/2015STRAJ044.

Council of Science Editors:

Benbarche S. Influence des cytokines immunitaires sur la mégacaryocytopoïèse et l'homéostasie des plaquettes sanguines : rôle de l'interleukine 21 : Effect of immune cytokines on megakaryocytopoiesis and platelet homeostasis : role of interleukin 21. [Doctoral Dissertation]. Université de Strasbourg; 2015. Available from: http://www.theses.fr/2015STRAJ044

University of Texas Southwestern Medical Center

27. Baughman, Ethan James. Neuroantigen-Specific CD8+ Regulatory T-Cell Function Is Deficient During Acute Exacerbation of Multiple Sclerosis.

Degree: 2013, University of Texas Southwestern Medical Center

URL: http://hdl.handle.net/2152.5/1703

► Multiple sclerosis (MS) is an inflammatory, demyelinating disease of the central nervous system (CNS). MS is thought to be T-cell-mediated, with prior research predominantly focusing… (more)

▼ Multiple sclerosis (MS) is an inflammatory, demyelinating disease of the central nervous system (CNS). MS is thought to be T-cell-mediated, with prior research predominantly focusing on CD4+ T-cells. There is a high prevalence of CNS-specific CD8+ T-cell responses in MS patients and healthy subjects. However, the role of neuroantigen-specific CD8+ T-cells in MS is poorly understood, with the prevalent notion that these may represent pathogenic T-cells. We show here that healthy subjects and MS patients demonstrate similar magnitudes of CD8+ and CD4+ T-cell responses to various antigenic stimuli. Interestingly, CD8+ T-cells specific for CNS autoantigens, but not those specific for control foreign antigens, exhibit immune regulatory ability, suppressing proliferation of CD4+CD25- T-cells when stimulated by their cognate antigen. While CD8+ T-cell-mediated immune suppression is similar between healthy subjects and clinically quiescent treatment-naïve MS patients, it is significantly deficient during acute exacerbation of MS. Of note, the recovery of neuroantigen-specific CD8+ T-cell suppression correlates with disease recovery post-relapse. In healthy adult subjects, we observed that the CD62L- subset of CD8+ T cells harbored increased CNS- and Copaxone-specific suppressive ability, when compared to the CD62L+ subset and bulk CD8+ T cells, and that the CD28+ subset of CD8+ T cells harbored increased global suppressive ability, when compared to the CD28- subset. In contrast, we observed CD8+ T cells from neonates harbored increased global suppressive ability in the CD28- subset. The mechanism of neuroantigen-specific suppression by CD8+ T cells was dependent upon HLA class I, IFN gamma, with possible partial involvement by NKG2D, PD-1, and IL-10. These studies reveal a novel immune suppressor function for neuroantigen-specific CD8+ T-cells that is clinically relevant in the maintenance of peripheral tolerance and the intrinsic regulation of MS immune pathology. Advisors/Committee Members: Karandikar, Nitin J., Street, Nancy E., Thiele, Dwain L., Forman, James, Greenberg, Benjamin M..

Subjects/Keywords: Autoantigens; CD8-Positive T-Lymphocytes; Multiple Sclerosis; T-Lymphocytes, Regulatory

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Baughman, E. J. (2013). Neuroantigen-Specific CD8+ Regulatory T-Cell Function Is Deficient During Acute Exacerbation of Multiple Sclerosis. (Thesis). University of Texas Southwestern Medical Center. Retrieved from http://hdl.handle.net/2152.5/1703

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Baughman, Ethan James. “Neuroantigen-Specific CD8+ Regulatory T-Cell Function Is Deficient During Acute Exacerbation of Multiple Sclerosis.” 2013. Thesis, University of Texas Southwestern Medical Center. Accessed January 20, 2021. http://hdl.handle.net/2152.5/1703.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Baughman, Ethan James. “Neuroantigen-Specific CD8+ Regulatory T-Cell Function Is Deficient During Acute Exacerbation of Multiple Sclerosis.” 2013. Web. 20 Jan 2021.

Vancouver:

Baughman EJ. Neuroantigen-Specific CD8+ Regulatory T-Cell Function Is Deficient During Acute Exacerbation of Multiple Sclerosis. [Internet] [Thesis]. University of Texas Southwestern Medical Center; 2013. [cited 2021 Jan 20]. Available from: http://hdl.handle.net/2152.5/1703.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Baughman EJ. Neuroantigen-Specific CD8+ Regulatory T-Cell Function Is Deficient During Acute Exacerbation of Multiple Sclerosis. [Thesis]. University of Texas Southwestern Medical Center; 2013. Available from: http://hdl.handle.net/2152.5/1703

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Texas Southwestern Medical Center

28. Ortega, Sterling Bolivar. Autoregulatory CD8 T-Cells Modulate CNS Autoimmune Disease by Targeting Encephalitogenic CD4 T-Cells.

Degree: 2013, University of Texas Southwestern Medical Center

URL: http://hdl.handle.net/2152.5/1221

► Multiple Sclerosis (MS) is a disease, which presents with neurological dysfunction and is believed to have an immunological etiology. Lesions in the central nervous system… (more)

▼ Multiple Sclerosis (MS) is a disease, which presents with neurological dysfunction and is believed to have an immunological etiology. Lesions in the central nervous system (CNS) are characterized by an inflammatory cellular infiltration and demyelination of neuronal axons. It is believed that myelin sheath-targeting CD4 T-cells are important mediators of this disease. While it is known that CD8 T-cells are present, oligoclonally expanded and are the predominant T-cell population in the MS CNS lesion, their antigen specificity and function remains to be elucidated. Using a murine model of MS, experimental autoimmune encephalomyelitis (EAE), we are currently evaluating the role of these poorly studied myelin antigen-specific CD8 T-cells in the context of this autoimmune disease. We have observed that myelin oligodendrocyte glycoprotein peptide (MOG₃₅₋₅₅)-specific CD8 T-cells do not mediate EAE, but in fact are capable of suppressing both de novo and established clinical disease. Corroborating these data, CD8-/- C57BL/6 mice are now shown to exhibit a more severe EAE. However, the characterization, mechanism of action and cellular targets of these autoreactive regulatory CD8 T-cells are still unknown. Initial observations revealed that disease ameliorating CD8 T-cells are not unique to MOG₃₅₋₅₅-induced EAE, as proteolipid protein peptide (PLP₁₇₈₋₁₉₁)-induced EAE in B6 and SJL mice strains were capable of generating disease ameliorating CNS antigen-specific CD8 T-cells. Autoreactive regulatory (autoregulatory) CD8 T-cells exhibit a central memory phenotype (CCR7+CD62L+CD44-) and produce IFN-γ and perforin. Disease suppression by these cells is dependent on recognition of cognate antigen in vivo within the context of MHC Class Ia. These cells do not traffic to the CNS upon transfer into naïve mice; however, they gain CNS access following induction of CNS inflammation, suggesting both a peripheral immune compartment and CNS mechanism of action. Interestingly, autoregulatory CD8 T-cell-mediated suppression is IFN-γ and perforin-dependent and can be augmented by IL-12 in vitro modulation. Next, we asked whether autoregulatory CD8 T-cells could directly target encephalitogenic myelin-specific CD4 T-cells. We now report that treatment with myelinspecific CD8 T-cells results in significantly attenuated adoptive (CD4 T-cell mediated) EAE. Moreover, increased disease severity in CD8-/- mice correlated with an increase in autoreactivity and inflammatory cytokine production by myelin-specific CD4 T-cells. This is reversible upon adoptive transfer of MOG₃₅₋₅₅-specific CD8 T-cells. Targeting of encephalitogenic CD4 T-cells by myelin-specific CD8 T-cells is sufficient, as induction of wildtype adoptive EAE in KᵇDᵇ-/- recipient mice could be suppressed. In vivo proliferation assays revealed a global suppression/cytotoxicity of MOG-specific CD4 T-cells. These studies define the immune regulatory function of autoreactive CD8 T-cells in EAE. Our results demonstrate that autoregulatory CD8 T-cells have an important disease ameliorating… Advisors/Committee Members: Karandikar, Nitin J..

Subjects/Keywords: CD4-Positive T-Lymphocytes; CD8-Positive T-Lymphocytes; Multiple Sclerosis

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Ortega, S. B. (2013). Autoregulatory CD8 T-Cells Modulate CNS Autoimmune Disease by Targeting Encephalitogenic CD4 T-Cells. (Thesis). University of Texas Southwestern Medical Center. Retrieved from http://hdl.handle.net/2152.5/1221

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Ortega, Sterling Bolivar. “Autoregulatory CD8 T-Cells Modulate CNS Autoimmune Disease by Targeting Encephalitogenic CD4 T-Cells.” 2013. Thesis, University of Texas Southwestern Medical Center. Accessed January 20, 2021. http://hdl.handle.net/2152.5/1221.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Ortega, Sterling Bolivar. “Autoregulatory CD8 T-Cells Modulate CNS Autoimmune Disease by Targeting Encephalitogenic CD4 T-Cells.” 2013. Web. 20 Jan 2021.

Vancouver:

Ortega SB. Autoregulatory CD8 T-Cells Modulate CNS Autoimmune Disease by Targeting Encephalitogenic CD4 T-Cells. [Internet] [Thesis]. University of Texas Southwestern Medical Center; 2013. [cited 2021 Jan 20]. Available from: http://hdl.handle.net/2152.5/1221.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Ortega SB. Autoregulatory CD8 T-Cells Modulate CNS Autoimmune Disease by Targeting Encephalitogenic CD4 T-Cells. [Thesis]. University of Texas Southwestern Medical Center; 2013. Available from: http://hdl.handle.net/2152.5/1221

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Université de Lorraine

29. Gaignier, Fanny. Modulation de l'immunité adaptative murine par la micropesanteur simulée, l'hypergravité ou les stress chroniques ultra légers : Modulation of murine adaptive immunity by simulated microgravity, hypergravity or chronic ultra mild stress.

Degree: Docteur es, Sciences de la vie et de la santé, 2014, Université de Lorraine

URL: http://www.theses.fr/2014LORR0171

►

Les vols spatiaux affaiblissent le système immunitaire. Les objectifs de cette thèse étaient de déterminer, à l’aide de modèles terrestres, les conséquences d’une exposition à… (more)

▼

Les vols spatiaux affaiblissent le système immunitaire. Les objectifs de cette thèse étaient de déterminer, à l’aide de modèles terrestres, les conséquences d’une exposition à la micropesanteur simulée, l’hypergravité ou des stress chroniques ultra légers sur l’immunité humorale murine. Nous avons ainsi montré que la suspension anti-Orthostatique, qui mime certains effets de la micropesanteur, induit une diminution de 59% du nombre de lymphocytes B spléniques et une inversion du rapport entre lymphocytes B et T. L’affaiblissement de la lymphopoïèse B mis en évidence par la diminution du nombre de progéniteurs lymphoïdes et des cellules pré-B en est probablement la cause. De plus, la position anti-Orthostatique entraîne, en l’absence de stress, une diminution de la réponse in vitro des lymphocytes B au LPS, plus importante que celle des lymphocytes T à la ConA, comme en hypergravité. Afin de déterminer les mécanismes responsables de la diminution de la réponse des lymphocytes B chez les souris hypergravitaires, les transcrits de gènes impliqués dans la voie TLR-4, le récepteur du LPS, ont été quantifiés. L’expression de plusieurs gènes de la voie MyD88-Dépendante est augmentée après 21 jours d’hypergravité, mais aucune modification n’a été observée dans les lymphocytes B stimulés avec du LPS. Enfin, la contribution de stress chroniques ultra légers a été évaluée. Les proportions de lymphocytes spléniques ne sont pas affectées par ces stress. Par contre, le taux d’IgA sériques s’est révélé augmenté et une baisse des cytokines pro-Inflammatoires de type Th1 a été observée comme chez les astronautes. Ainsi, ces stress n’expliquent pas complètement l’impact négatif des vols spatiaux sur l’immunité humorale. Ces recherches sont importantes pour identifier la/les cause(s) de l’altération de l’immunité humorale en vol, afin de tester/développer des contremesures efficaces pour renforcer le système immunitaire des astronautes, mais également de personnes stressées ou âgées

Spaceflight weaken the immune system. The aims of this thesis were to determine the effects of exposure to simulated microgravity, hypergravity or chronic ultra-Mild stress on murine humoral immunity, using ground-Based models. We were able to show that the anti-Orthostatic suspension, that mimics some of the effects of microgravity, caused a 59% decrease of the number of splenic B cells and an inversion of the ratio between B and T lymphocytes. A decrease in B lymphopoiesis, as evidenced by the decrease of lymphoid progenitors and pre-B cells is likely the cause. Furthermore, we showed that the anti-Orthostatic position, in the absence of stress, leads to a decreased in vitro response of B cells to LPS, more important than the one of T cells to ConA, as did hypergravity exposure. To determine the mechanisms responsible of the decreased response of B cells from hypergravity mice, transcripts encoding genes involved in the TLR-4 signaling pathway, the LPS receptor on B cells, have been quantified. The expression of several genes of the MyD88-Dependent…

Advisors/Committee Members: Frippiat, Jean-Pol (thesis director), Legrand-Frossi, Christine (thesis director).

Subjects/Keywords: Souris; Lymphocytes; Micropesanteur; Hypergravité; Stress; Mouse; Lymphocytes; Microgravity; Hypergravity; Stress; 616.079

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Gaignier, F. (2014). Modulation de l'immunité adaptative murine par la micropesanteur simulée, l'hypergravité ou les stress chroniques ultra légers : Modulation of murine adaptive immunity by simulated microgravity, hypergravity or chronic ultra mild stress. (Doctoral Dissertation). Université de Lorraine. Retrieved from http://www.theses.fr/2014LORR0171

Chicago Manual of Style (16^th Edition):

Gaignier, Fanny. “Modulation de l'immunité adaptative murine par la micropesanteur simulée, l'hypergravité ou les stress chroniques ultra légers : Modulation of murine adaptive immunity by simulated microgravity, hypergravity or chronic ultra mild stress.” 2014. Doctoral Dissertation, Université de Lorraine. Accessed January 20, 2021. http://www.theses.fr/2014LORR0171.

MLA Handbook (7^th Edition):

Gaignier, Fanny. “Modulation de l'immunité adaptative murine par la micropesanteur simulée, l'hypergravité ou les stress chroniques ultra légers : Modulation of murine adaptive immunity by simulated microgravity, hypergravity or chronic ultra mild stress.” 2014. Web. 20 Jan 2021.

Vancouver:

Gaignier F. Modulation de l'immunité adaptative murine par la micropesanteur simulée, l'hypergravité ou les stress chroniques ultra légers : Modulation of murine adaptive immunity by simulated microgravity, hypergravity or chronic ultra mild stress. [Internet] [Doctoral dissertation]. Université de Lorraine; 2014. [cited 2021 Jan 20]. Available from: http://www.theses.fr/2014LORR0171.

Council of Science Editors:

Gaignier F. Modulation de l'immunité adaptative murine par la micropesanteur simulée, l'hypergravité ou les stress chroniques ultra légers : Modulation of murine adaptive immunity by simulated microgravity, hypergravity or chronic ultra mild stress. [Doctoral Dissertation]. Université de Lorraine; 2014. Available from: http://www.theses.fr/2014LORR0171

30. Fali, Tinhinane. Etude de la signalisation calcique du lymphocyte B en pathologie : No.

Degree: Docteur es, Biologie et santé. Immunologie, 2013, Brest

URL: http://www.theses.fr/2013BRES0086

►

La signalisation calcique est un mécanisme physiologique essentiel pour l’homéostasie cellulaire, et en particulier pour les cellules immunitaires telles que les lymphocytes B (LB). Chez… (more)

▼

La signalisation calcique est un mécanisme physiologique essentiel pour l’homéostasie cellulaire, et en particulier pour les cellules immunitaires telles que les lymphocytes B (LB). Chez l’homme, la caractérisation des molécules impliquées dans la signalisation calcique des LB est encore restreinte. Nous avons donc choisi d’étudier les molécules STIM1, STIM2, Orai1 et TRPC1 afin de rendre compte des modifications de la signalisation calcique observées dans deux modèles physiopathologiques : le Lupus Erythémateux Systémique (LES) et la Leucémie Lymphoïde Chronique (LLC). Pour les LB de LES, par rapport à des LB de témoins, ce travail a permis de montrer que la surexpression de la molécule STIM1 dans les LB de LES, surtout au stade des LB transitionnels, était associée avec un influx constitutif et extracellulaire de calcium et que cet influx était responsable d’une phosphorylation modérée de la MAPK Erk1/2 dans les cellules au repos. Cependant, cet effet positif est contrebalancé par un défaut de l’influx SOCE (strore operated calcium entry) et d’un défaut d’activation de la phosphorylation de la MAPK Erk après stimulation du récepteur à l’antigène des LB (BCR). Les effets positifs et négatifs observés sur la phosphorylation d.Erk1/2 sont directement attribuables au niveau d’expression de STIM1 comme nous l’avons démontré en sur-exprimant STIM1 dans des LB de témoins (vecteur pSTIM1-STIM1) ou en ayant recours à un siRNA spécifique de STIM1 dans les LB de LES. Le défaut d’activité régulatrice des LB de LES sur la prolifération des LT dans un modèle de co-culture autologue LT/LB en présence de CpG (activation du TLR9 des LB), et d’anticorps anti-CD3/CD28 (activation des LT) est levé en inhibant l’expression de STIM1 dans ces cellules ce qui permet de restaurer la production d.IL-10 par les LB de LES et la génération de LT régulateurs. Le défaut d’activation de la voie Erk/DNMT1/méthylation de l’ADN dans les LB de LES semble également contrôlée par le niveau d’expression de la molécule STIM1.Pour les LB CD5+ de LLC, l’analyse des partenaires calciques nous a permis de distinguer deux groupes de patients : un premier groupe qui exprime le complexe membranaire STIM1/TRPC1/Orai1 et un second groupe qui n’exprime qu’Orai1. La présence du complexe membranaire STIM1/TRPC1/Orai1 est associée avec un influx constitutif de calcium, la phosphorylation d’Erk1/2, et un défaut d’activation du signal SOCE après stimulation du BCR. Le rôle de la molécule CD5 sur l’influx constitutif des LLC du groupe I a été démontré en utilisant des siRNA spécifiques pourCD5 et en analysant la lignée B humaine Jok-1 transfectée avec CD5. Enfin, l’utilisation d’un anticorps anti-STIM1 est capable d’induire l’apoptose in vitro des LB de LLC du groupe I, alors que l’anticorps anti-CD20, rituximab, n’est efficace que sur les LLC du groupe II.En conclusion, cette étude ouvre de nouvelles perspectives diagnostiques et thérapeutiques en auto-immunité dans le LES et en cancérologie dans la LLC.

Calcium signaling is a central mechanism for cellular…

Advisors/Committee Members: Renaudineau, Yves (thesis director).

Subjects/Keywords: Lymphocytes B; Signalisation calcique; Lymphocytes B; Calcium signalisation

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Fali, T. (2013). Etude de la signalisation calcique du lymphocyte B en pathologie : No. (Doctoral Dissertation). Brest. Retrieved from http://www.theses.fr/2013BRES0086

Chicago Manual of Style (16^th Edition):

Fali, Tinhinane. “Etude de la signalisation calcique du lymphocyte B en pathologie : No.” 2013. Doctoral Dissertation, Brest. Accessed January 20, 2021. http://www.theses.fr/2013BRES0086.

MLA Handbook (7^th Edition):

Fali, Tinhinane. “Etude de la signalisation calcique du lymphocyte B en pathologie : No.” 2013. Web. 20 Jan 2021.

Vancouver:

Fali T. Etude de la signalisation calcique du lymphocyte B en pathologie : No. [Internet] [Doctoral dissertation]. Brest; 2013. [cited 2021 Jan 20]. Available from: http://www.theses.fr/2013BRES0086.

Council of Science Editors:

Fali T. Etude de la signalisation calcique du lymphocyte B en pathologie : No. [Doctoral Dissertation]. Brest; 2013. Available from: http://www.theses.fr/2013BRES0086

Open Access Theses and Dissertations