subject:(lymphocytes)

1. Al-Omairi, Jaafar Ghadeer Khudhair. Functional study of lymphoid specific enhancers : Etude fonctionnelle des enhancers lymphoides.

Degree: Docteur es, Génétique, 2017, Aix Marseille Université

URL: http://www.theses.fr/2017AIXM0474

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Les amplificateurs (aussi appelés par le terme anglais enhancers) ont été initialement identifiés comme des séquences d'ADN agissant en cis qui augmentent la transcription d'une… (more)

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Les amplificateurs (aussi appelés par le terme anglais enhancers) ont été initialement identifiés comme des séquences d'ADN agissant en cis qui augmentent la transcription d'une manière qui est indépendante de leur orientation et de leur distance par rapport au site d’initiation de la transcription. En outre, les gènes d'identité cellulaire sont souvent associés à des regroupements ou clusters d’enhancers, structures également appelées super-enhancers, censés assurer une régulation correcte de l'expression des gènes tout au long du développement et de la différenciation des cellules. Pour mieux comprendre la régulation des gènes à partir de ces réseaux régulateurs complexes, nous avons étudié la régulation du gène Ikzf1 qui code pour un facteur de transcription essentiel à la différenciation lymphoïde et également impliqué dans la leucémogenèse. En combinant différents types de données épigénomiques, nous avons privilégié l’étude d’un élément enhancer situé à 120 kb en amont du gène Ikzf1. Nous avons trouvé que la délétion de l'enhancer IkE120 entraine une réduction significative de l'ARNm d’Ikzf1. Cependant, nous avons observé que la transcription immature ainsi que l’usage des promoteurs et exons alternatifs d’Ikzf1 sont différemment affectée dans les cellules délitées par IKE120. Ces résultats semblent indiquer que l'élément IkE120 pourrait avoir des fonctions supplémentaires au-delà de la seule régulation de l'initiation de la transcription.

Enhancers were originally identified as cis-acting DNA sequences that increase transcription in a manner that is independent of their orientation and distance relative to the RNA start site. In addition, cell identity genes are often associated with cluster of enhancers, also termed super-enhancers, which are believed to ensure proper regulation of gene expression throughout cell development and differentiation. To better understand gene regulation based on these complex regulatory networks, we studied the regulation of the Ikzf1 gene which encoded for a lymphoid-specific transcription factor essential for lymphoid differentiation and also involved in leukemogenesis. By combining different epigenomics data sets we prioritize an enhancer element located 120 kb upstream the IKZF1 gene. We found that deletion of the E120 enhancer resulted in significant reduction of Ikzf1 mRNA. However, we observed that immature transcription, promoter and exon usage were differentially affected in the IKE120-deleted cells. The results indicated that E120 element might have additional functions over solely regulating transcription initiation. We suggest that expression of some tissue-specific and cell identity genes might, at least partially, be regulated at the level of mRNA maturation and that components of enhancer’s clusters are directly involved in this process.

Advisors/Committee Members: Spicuglia, Salvatore (thesis director).

Subjects/Keywords: Promoteur; Enhancer; Lymphocytes; Promoter; Enhancer; Lymphocytes; 572

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APA (6^th Edition):

Al-Omairi, J. G. K. (2017). Functional study of lymphoid specific enhancers : Etude fonctionnelle des enhancers lymphoides. (Doctoral Dissertation). Aix Marseille Université. Retrieved from http://www.theses.fr/2017AIXM0474

Chicago Manual of Style (16^th Edition):

Al-Omairi, Jaafar Ghadeer Khudhair. “Functional study of lymphoid specific enhancers : Etude fonctionnelle des enhancers lymphoides.” 2017. Doctoral Dissertation, Aix Marseille Université. Accessed June 24, 2019. http://www.theses.fr/2017AIXM0474.

MLA Handbook (7^th Edition):

Al-Omairi, Jaafar Ghadeer Khudhair. “Functional study of lymphoid specific enhancers : Etude fonctionnelle des enhancers lymphoides.” 2017. Web. 24 Jun 2019.

Vancouver:

Al-Omairi JGK. Functional study of lymphoid specific enhancers : Etude fonctionnelle des enhancers lymphoides. [Internet] [Doctoral dissertation]. Aix Marseille Université 2017. [cited 2019 Jun 24]. Available from: http://www.theses.fr/2017AIXM0474.

Council of Science Editors:

Al-Omairi JGK. Functional study of lymphoid specific enhancers : Etude fonctionnelle des enhancers lymphoides. [Doctoral Dissertation]. Aix Marseille Université 2017. Available from: http://www.theses.fr/2017AIXM0474

Université Laval

2. Naouel Hachehouche, Lamia. Expression du récepteur à domaine discoïdine 1 chez le lymphocyte T et son implication dans la migration cellulaire.

Degree: 2009, Université Laval

URL: http://hdl.handle.net/20.500.11794/20908

► La migration des lymphocytes T dans les tissus interstitiels est régulée par la matrice extracellulaire dont le collagène type l est la composante majeure. Nous… (more)

Subjects/Keywords: Lymphocytes T; Lymphocytes – Récepteurs; Cellules – Migration

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APA (6^th Edition):

Naouel Hachehouche, L. (2009). Expression du récepteur à domaine discoïdine 1 chez le lymphocyte T et son implication dans la migration cellulaire. (Thesis). Université Laval. Retrieved from http://hdl.handle.net/20.500.11794/20908

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Naouel Hachehouche, Lamia. “Expression du récepteur à domaine discoïdine 1 chez le lymphocyte T et son implication dans la migration cellulaire.” 2009. Thesis, Université Laval. Accessed June 24, 2019. http://hdl.handle.net/20.500.11794/20908.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Naouel Hachehouche, Lamia. “Expression du récepteur à domaine discoïdine 1 chez le lymphocyte T et son implication dans la migration cellulaire.” 2009. Web. 24 Jun 2019.

Vancouver:

Naouel Hachehouche L. Expression du récepteur à domaine discoïdine 1 chez le lymphocyte T et son implication dans la migration cellulaire. [Internet] [Thesis]. Université Laval; 2009. [cited 2019 Jun 24]. Available from: http://hdl.handle.net/20.500.11794/20908.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Naouel Hachehouche L. Expression du récepteur à domaine discoïdine 1 chez le lymphocyte T et son implication dans la migration cellulaire. [Thesis]. Université Laval; 2009. Available from: http://hdl.handle.net/20.500.11794/20908

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Université Laval

3. Paquin-Proulx, Dominic. Effet des immunoglobulines intraveineuses sur les lymphocytes B : mécanismes d'action et récepteurs impliqués.

Degree: 2010, Université Laval

URL: http://hdl.handle.net/20.500.11794/21968

► Le plasma récupéré des dons de sang est utilisé pour produire annuellement plusieurs milliers de kg de préparations thérapeutiques d'immunoglobulines pour injection intraveineuse (IglV) qui… (more)

▼ Le plasma récupéré des dons de sang est utilisé pour produire annuellement plusieurs milliers de kg de préparations thérapeutiques d'immunoglobulines pour injection intraveineuse (IglV) qui sont utilisées pour le traitement de patients souffrant d'immunodéficiences primaires ou secondaires. Depuis quelques années, de très fortes doses d'IglV sont également utilisées pour traiter un nombre croissant de maladies autoimmunes, augmentant grandement les risques de pénurie de ce produit pour lequel il n'existe à l'heure actuelle aucun substitut. Il a été rapporté que le traitement in vitro des lymphocytes B humains aux IgPV induit une baisse de leur prolifération ainsi qu'une augmentation de la sécrétion d'IgG. Mes travaux de thèse ont porté sur l'étude des mécanismes par lesquels les IglV agissent sur les lymphocytes B. Dans un premier temps, nous avons utilisé une approche d'immunoprecipitation suivie d'analyses par spectrométrie de masse afin d'identifier les molécules ciblées par les IgPV à la surface des lymphocytes B. Comme prévu, nous avons identifié plusieurs protéines membranaires ou associées à la membrane plasmique telles que le CD91, les molécules du CMH I et II ainsi que les prohibitines 1 et 2. Cependant, des protéines strictement intracellulaires ont aussi été identifiées, telles que l'endoplasmine et le LYST (Lysosomal-trafficking regulator). Ces résultats nous ont permis de démontrer que les IglV étaient internalisées de manière spécifique et spontanée par les lymphocytes B activés in vitro et par les cellules myéloïdes dans le sang total. L'identification de molécules impliquées dans le processus de la présentation antigénique, comme les complexes majeurs d'histocompatibilité (CMH) I et II, nous a conduit à émettre l'hypothèse que les IgPV pouvaient interférer avec ce processus. À l'aide d'un système in vitro de présentation antigénique dépendant du CMH H, nous avons démontré que les IglV inhibaient la présentation d'antigène dépendante et indépendante du récepteur antigénique (BCR). Il a aussi pu être établi que cet effet n'était pas dû à une modulation du niveau de CMH II exprimé à la surface des lymphocytes B ni dépendant du récepteur inhibiteur pour la partie constante des IgG, le FcyRIIb. Nous avons donc proposé un mécanisme dépendant de l'interaction avec des protéines intracellulaires impliquées dans le processus de la présentation antigénique afin d'expliquer cet effet des IglV. Par la suite, à l'aide d'une lignée cellulaire déficiente pour l'expression du CD91, il nous a été possible de démontrer l'implication de ce récepteur dans l'internalisation spontanée des IglV. De plus, nous avons observé que le traitement aux IglV induit une phosphorylation de la partie intracellulaire du CD91 sur la tyrosine 4507. Nos résultats suggèrent aussi que le CD91 pourrait permettre la transcytose des IglV à travers la barrière hémato-encéphalique. En résumé, mes travaux de thèse ont identifié le CD91 comme étant impliqué dans le processus d'internalisation spontanée des IglV et suggèrent que cette… Advisors/Committee Members: Darveau, André, Bazin, Renée.

Subjects/Keywords: Lymphocytes B; Immunoglobulines

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Paquin-Proulx, D. (2010). Effet des immunoglobulines intraveineuses sur les lymphocytes B : mécanismes d'action et récepteurs impliqués. (Thesis). Université Laval. Retrieved from http://hdl.handle.net/20.500.11794/21968

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Paquin-Proulx, Dominic. “Effet des immunoglobulines intraveineuses sur les lymphocytes B : mécanismes d'action et récepteurs impliqués.” 2010. Thesis, Université Laval. Accessed June 24, 2019. http://hdl.handle.net/20.500.11794/21968.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Paquin-Proulx, Dominic. “Effet des immunoglobulines intraveineuses sur les lymphocytes B : mécanismes d'action et récepteurs impliqués.” 2010. Web. 24 Jun 2019.

Vancouver:

Paquin-Proulx D. Effet des immunoglobulines intraveineuses sur les lymphocytes B : mécanismes d'action et récepteurs impliqués. [Internet] [Thesis]. Université Laval; 2010. [cited 2019 Jun 24]. Available from: http://hdl.handle.net/20.500.11794/21968.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Paquin-Proulx D. Effet des immunoglobulines intraveineuses sur les lymphocytes B : mécanismes d'action et récepteurs impliqués. [Thesis]. Université Laval; 2010. Available from: http://hdl.handle.net/20.500.11794/21968

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Central Florida

4. Kittipatarin, Christina. Interleukin-7 Differentially Regulates The Activation, Proliferation, And Homing Of T-cells: Implications For Immunotherapy.

Degree: 2010, University of Central Florida

URL: https://stars.library.ucf.edu/etd/4330

► Interleukin-7 (IL-7) is an essential lymphocyte growth factor required for the survival and proliferation of mature T-cells. As a therapeutic agent, IL-7 has the potential… (more)

▼ Interleukin-7 (IL-7) is an essential lymphocyte growth factor required for the survival and proliferation of mature T-cells. As a therapeutic agent, IL-7 has the potential to restore T-cell numbers following immune depletion and to promote immunity against cancers. While the survival function of IL-7 is well established, less is known about how it supports T-cell expansion, a critical feature of the immune response. To study the biological effects of IL-7 on T-cell growth, we developed an in vitro culture technique to expand T-cells ex vivo. A significant finding from our studies is that IL-7 did not induce the expansion of all T-cells, indicating that there are inherent differences in the response of individual T-cell subsets to IL-7. Culture with high doses of IL-7 (>150 ng/ml) preferentially expanded CD8 T-cells, but lead to the dramatic loss of CD4 T-cells which favored growth in lower dosages of IL-7 (>10 ng/ml). This effect was due to the regulation of LCK, a kinase predominantly associated with the CD4 co-receptor. We found that transgenic expression of the CD4 co-receptor onto CD8 T-cells promoted their growth in lower concentrations of IL-7. Conversely, inhibition of LCK activity in CD4 T-cells restored their responsiveness to high doses of IL-7 as indicated by the activation of the transcription factor STAT5, in a manner similar to CD8 T-cells. Interestingly, not all CD8 T-cells expanded in high doses of IL-7 and this effect was specific to CD8 T-cells that expressed an activated memory phenotype. We found that IL-7 promoted the proliferation of CD8 T-cells through Cdc25A, a phosphatase required for cell cycle progression. Expression of a constitutively active Cdc25A could maintain T-cell survival and proliferation in the absence of IL-7, demonstrating that Cdc25A is a crucial transducer of IL-7 growth signals. Inhibition of Cdc25A was sufficient to decrease proliferation and down-regulate the expression of activation/ memory markers on CD8 T-cells in the presence of IL-7. Upon further study, we identified a novel role for IL-7 through Cdc25A in the regulation of CD62L, an adhesion molecule required for lymph node entry. Culture with high doses of IL-7 down-regulated the expression of CD62L, suggesting that high doses of IL-7 could affect the ability of T-cells to enter or re-enter the lymph nodes. Collectively, our findings demonstrate that IL-7 administration at the supraphysiological doses currently used in the clinical trials could have a negative impact on the growth of CD4 T-cells and the homing of CD8 T-cells to the lymph nodes, effects which can impede the generation of an effective immune response. Advisors/Committee Members: Khaled, Annette.

Subjects/Keywords: cytokine; lymphocytes; proliferation

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Kittipatarin, C. (2010). Interleukin-7 Differentially Regulates The Activation, Proliferation, And Homing Of T-cells: Implications For Immunotherapy. (Doctoral Dissertation). University of Central Florida. Retrieved from https://stars.library.ucf.edu/etd/4330

Chicago Manual of Style (16^th Edition):

Kittipatarin, Christina. “Interleukin-7 Differentially Regulates The Activation, Proliferation, And Homing Of T-cells: Implications For Immunotherapy.” 2010. Doctoral Dissertation, University of Central Florida. Accessed June 24, 2019. https://stars.library.ucf.edu/etd/4330.

MLA Handbook (7^th Edition):

Kittipatarin, Christina. “Interleukin-7 Differentially Regulates The Activation, Proliferation, And Homing Of T-cells: Implications For Immunotherapy.” 2010. Web. 24 Jun 2019.

Vancouver:

Kittipatarin C. Interleukin-7 Differentially Regulates The Activation, Proliferation, And Homing Of T-cells: Implications For Immunotherapy. [Internet] [Doctoral dissertation]. University of Central Florida; 2010. [cited 2019 Jun 24]. Available from: https://stars.library.ucf.edu/etd/4330.

Council of Science Editors:

Kittipatarin C. Interleukin-7 Differentially Regulates The Activation, Proliferation, And Homing Of T-cells: Implications For Immunotherapy. [Doctoral Dissertation]. University of Central Florida; 2010. Available from: https://stars.library.ucf.edu/etd/4330

The Ohio State University

5. Waksal, Samuel David. In vitro studies on thymus derived lymphocytes : differentiation of T-lymphocytes and their function in tumor destruction.

Degree: PhD, Graduate School, 1974, The Ohio State University

URL: http://rave.ohiolink.edu/etdc/view?acc_num=osu1486990930833541

Subjects/Keywords: Biology; Lymphocytes

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Waksal, S. D. (1974). In vitro studies on thymus derived lymphocytes : differentiation of T-lymphocytes and their function in tumor destruction. (Doctoral Dissertation). The Ohio State University. Retrieved from http://rave.ohiolink.edu/etdc/view?acc_num=osu1486990930833541

Chicago Manual of Style (16^th Edition):

Waksal, Samuel David. “In vitro studies on thymus derived lymphocytes : differentiation of T-lymphocytes and their function in tumor destruction.” 1974. Doctoral Dissertation, The Ohio State University. Accessed June 24, 2019. http://rave.ohiolink.edu/etdc/view?acc_num=osu1486990930833541.

MLA Handbook (7^th Edition):

Waksal, Samuel David. “In vitro studies on thymus derived lymphocytes : differentiation of T-lymphocytes and their function in tumor destruction.” 1974. Web. 24 Jun 2019.

Vancouver:

Waksal SD. In vitro studies on thymus derived lymphocytes : differentiation of T-lymphocytes and their function in tumor destruction. [Internet] [Doctoral dissertation]. The Ohio State University; 1974. [cited 2019 Jun 24]. Available from: http://rave.ohiolink.edu/etdc/view?acc_num=osu1486990930833541.

Council of Science Editors:

Waksal SD. In vitro studies on thymus derived lymphocytes : differentiation of T-lymphocytes and their function in tumor destruction. [Doctoral Dissertation]. The Ohio State University; 1974. Available from: http://rave.ohiolink.edu/etdc/view?acc_num=osu1486990930833541

The Ohio State University

6. Cannon, Marvin Samuel. A morphological cytochemical and statistical approach to the classification of lymphocytes.

Degree: PhD, Graduate School, 1969, The Ohio State University

URL: http://rave.ohiolink.edu/etdc/view?acc_num=osu1486654338997873

Subjects/Keywords: Biology; Lymphocytes

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Cannon, M. S. (1969). A morphological cytochemical and statistical approach to the classification of lymphocytes. (Doctoral Dissertation). The Ohio State University. Retrieved from http://rave.ohiolink.edu/etdc/view?acc_num=osu1486654338997873

Chicago Manual of Style (16^th Edition):

Cannon, Marvin Samuel. “A morphological cytochemical and statistical approach to the classification of lymphocytes.” 1969. Doctoral Dissertation, The Ohio State University. Accessed June 24, 2019. http://rave.ohiolink.edu/etdc/view?acc_num=osu1486654338997873.

MLA Handbook (7^th Edition):

Cannon, Marvin Samuel. “A morphological cytochemical and statistical approach to the classification of lymphocytes.” 1969. Web. 24 Jun 2019.

Vancouver:

Cannon MS. A morphological cytochemical and statistical approach to the classification of lymphocytes. [Internet] [Doctoral dissertation]. The Ohio State University; 1969. [cited 2019 Jun 24]. Available from: http://rave.ohiolink.edu/etdc/view?acc_num=osu1486654338997873.

Council of Science Editors:

Cannon MS. A morphological cytochemical and statistical approach to the classification of lymphocytes. [Doctoral Dissertation]. The Ohio State University; 1969. Available from: http://rave.ohiolink.edu/etdc/view?acc_num=osu1486654338997873

Georgia State University

7. Murali, Vineeth Kumar. Adenovirus Death Protein: The Switch Between Lytic and Persistent Infections in Lymphocytes?.

Degree: MS, Biology, 2012, Georgia State University

URL: https://scholarworks.gsu.edu/biology_theses/41

► ABSTRACT Adenovirus Death Protein (ADP) expression during late stages of a lytic infection releases mature virions to promote viral spread, thus leading to death… (more)

Subjects/Keywords: Adenovirus; ADP; Persistent infection; Lymphocytes

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APA (6^th Edition):

Murali, V. K. (2012). Adenovirus Death Protein: The Switch Between Lytic and Persistent Infections in Lymphocytes?. (Thesis). Georgia State University. Retrieved from https://scholarworks.gsu.edu/biology_theses/41

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Murali, Vineeth Kumar. “Adenovirus Death Protein: The Switch Between Lytic and Persistent Infections in Lymphocytes?.” 2012. Thesis, Georgia State University. Accessed June 24, 2019. https://scholarworks.gsu.edu/biology_theses/41.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Murali, Vineeth Kumar. “Adenovirus Death Protein: The Switch Between Lytic and Persistent Infections in Lymphocytes?.” 2012. Web. 24 Jun 2019.

Vancouver:

Murali VK. Adenovirus Death Protein: The Switch Between Lytic and Persistent Infections in Lymphocytes?. [Internet] [Thesis]. Georgia State University; 2012. [cited 2019 Jun 24]. Available from: https://scholarworks.gsu.edu/biology_theses/41.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Murali VK. Adenovirus Death Protein: The Switch Between Lytic and Persistent Infections in Lymphocytes?. [Thesis]. Georgia State University; 2012. Available from: https://scholarworks.gsu.edu/biology_theses/41

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Queens University

8. Seaward, Alexandra Victoria Catherine. Effects of Diabetes on Lymphocyte Phenotype and Function During Pregnancy .

Degree: Anatomy and Cell Biology, 2011, Queens University

URL: http://hdl.handle.net/1974/6299

► Diabetic pregnant women have an increased risk of developing pre-eclampsia, a late gestational syndrome, although the reason for this gain is unknown. Placental pathology in… (more)

▼ Diabetic pregnant women have an increased risk of developing pre-eclampsia, a late gestational syndrome, although the reason for this gain is unknown. Placental pathology in pre-eclampsia is linked with insufficient spiral arterial modification, a process triggered by uterine natural killer (uNK) cells, an abundant pro-angiogenic cell type found in early pregnancy decidua (endometrium of the pregnant uterus). UNK cells effect spiral arterial modification, the blood supply to the placenta, through the release of interferon gamma (Ifng), a pro-inflammatory cytokine. Peripheral blood precursors of uNK cells employ a unique pattern of homing molecules to traffic to the decidua. The goal of this thesis was to advance the understanding of how homing and functions of uNK cell precursors might be modified in diabetic pregnancy. Studies employed both murine and human models. Pregnancies were studied microscopically in normoglycemic (n-) and hyperglycemic (d-) non-obese diabetic (NOD) and NOD.Ifng-/- (NOD strain with a genetic deletion of Ifng) mice. Pre-implantation embryo development was impaired in n- and d-NOD.Ifng-/- mice. Hyperglycemia decreased both numbers of uNK cells and spiral artery remodelling within d-NOD and d-NOD.Ifng-/- decidua. This decrease in spiral artery remodelling was independent of Ifng and linked with hypertrophy of smooth muscle in implantation sites. Blood leukocytes from control and diabetic pregnant women were evaluated for adhesive function and expression of key homing molecules. Diabetic leukocytes had decreased CD56+ (uNK cell lineage) cell adhesion to decidua, increased CD56+ cell adhesion to pancreas, and comparable adhesion to lymph node compared with control leukocytes, suggesting impaired decidual homing in vivo. Of 8 lymphocyte subsets resolved by flow cytometry, type 1 cytokine CD56bright cells expressed appropriate homing molecules most abundantly. Diabetes did not alter levels of expression of these receptors. These data show that diabetes alters the potential capacity for decidual homing of pre-uNK cells but that this is not achieved through reduction in levels of key homing molecules. Diabetes also reduced spiral arterial modification in mice through hypertrophy of smooth muscle. The reproductive challenges of diabetic women who have co-morbid immunological diseases merit further study.

Subjects/Keywords: Diabetes; Immunology; Lymphocytes; Pregnancy

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Seaward, A. V. C. (2011). Effects of Diabetes on Lymphocyte Phenotype and Function During Pregnancy . (Thesis). Queens University. Retrieved from http://hdl.handle.net/1974/6299

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Seaward, Alexandra Victoria Catherine. “Effects of Diabetes on Lymphocyte Phenotype and Function During Pregnancy .” 2011. Thesis, Queens University. Accessed June 24, 2019. http://hdl.handle.net/1974/6299.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Seaward, Alexandra Victoria Catherine. “Effects of Diabetes on Lymphocyte Phenotype and Function During Pregnancy .” 2011. Web. 24 Jun 2019.

Vancouver:

Seaward AVC. Effects of Diabetes on Lymphocyte Phenotype and Function During Pregnancy . [Internet] [Thesis]. Queens University; 2011. [cited 2019 Jun 24]. Available from: http://hdl.handle.net/1974/6299.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Seaward AVC. Effects of Diabetes on Lymphocyte Phenotype and Function During Pregnancy . [Thesis]. Queens University; 2011. Available from: http://hdl.handle.net/1974/6299

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Hong Kong University of Science and Technology

9. Ye, Mulin. Initial study of the role of native interleukin-15 in generating tumor-specific cytotoxic T lymphocytes in a dendritic cell-mediated ex vivo system.

Degree: 2011, Hong Kong University of Science and Technology

URL: https://doi.org/10.14711/thesis-b1155457 ; http://repository.ust.hk/ir/bitstream/1783.1-7915/1/th_redirect.html

► Adoptive cell transfer (ACT) immunotherapy using ex vivo expanded tumor-specificcytotoxic T lymphocytes (CTLs) is one of the most promising approaches for treating cancers. The quality… (more)

Subjects/Keywords: Lymphocytes; Interleukins; Cellular therapy

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Ye, M. (2011). Initial study of the role of native interleukin-15 in generating tumor-specific cytotoxic T lymphocytes in a dendritic cell-mediated ex vivo system. (Thesis). Hong Kong University of Science and Technology. Retrieved from https://doi.org/10.14711/thesis-b1155457 ; http://repository.ust.hk/ir/bitstream/1783.1-7915/1/th_redirect.html

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Ye, Mulin. “Initial study of the role of native interleukin-15 in generating tumor-specific cytotoxic T lymphocytes in a dendritic cell-mediated ex vivo system.” 2011. Thesis, Hong Kong University of Science and Technology. Accessed June 24, 2019. https://doi.org/10.14711/thesis-b1155457 ; http://repository.ust.hk/ir/bitstream/1783.1-7915/1/th_redirect.html.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Ye, Mulin. “Initial study of the role of native interleukin-15 in generating tumor-specific cytotoxic T lymphocytes in a dendritic cell-mediated ex vivo system.” 2011. Web. 24 Jun 2019.

Vancouver:

Ye M. Initial study of the role of native interleukin-15 in generating tumor-specific cytotoxic T lymphocytes in a dendritic cell-mediated ex vivo system. [Internet] [Thesis]. Hong Kong University of Science and Technology; 2011. [cited 2019 Jun 24]. Available from: https://doi.org/10.14711/thesis-b1155457 ; http://repository.ust.hk/ir/bitstream/1783.1-7915/1/th_redirect.html.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Ye M. Initial study of the role of native interleukin-15 in generating tumor-specific cytotoxic T lymphocytes in a dendritic cell-mediated ex vivo system. [Thesis]. Hong Kong University of Science and Technology; 2011. Available from: https://doi.org/10.14711/thesis-b1155457 ; http://repository.ust.hk/ir/bitstream/1783.1-7915/1/th_redirect.html

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Hong Kong University of Science and Technology

10. Ke, Yan. Study of NY-ESO-1 specific cytotoxic T lymphocytes ex vivo.

Degree: 2010, Hong Kong University of Science and Technology

URL: https://doi.org/10.14711/thesis-b1115986 ; http://repository.ust.hk/ir/bitstream/1783.1-7003/1/th_redirect.html

► Adoptive T cell transfer therapy is one of the most promising cancer immunotherapeutic approaches. In order to provide adequate and efficient T cells for cancer… (more)

Subjects/Keywords: Antigens – Analysis; T cells; Lymphocytes

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Ke, Y. (2010). Study of NY-ESO-1 specific cytotoxic T lymphocytes ex vivo. (Thesis). Hong Kong University of Science and Technology. Retrieved from https://doi.org/10.14711/thesis-b1115986 ; http://repository.ust.hk/ir/bitstream/1783.1-7003/1/th_redirect.html

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Ke, Yan. “Study of NY-ESO-1 specific cytotoxic T lymphocytes ex vivo.” 2010. Thesis, Hong Kong University of Science and Technology. Accessed June 24, 2019. https://doi.org/10.14711/thesis-b1115986 ; http://repository.ust.hk/ir/bitstream/1783.1-7003/1/th_redirect.html.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Ke, Yan. “Study of NY-ESO-1 specific cytotoxic T lymphocytes ex vivo.” 2010. Web. 24 Jun 2019.

Vancouver:

Ke Y. Study of NY-ESO-1 specific cytotoxic T lymphocytes ex vivo. [Internet] [Thesis]. Hong Kong University of Science and Technology; 2010. [cited 2019 Jun 24]. Available from: https://doi.org/10.14711/thesis-b1115986 ; http://repository.ust.hk/ir/bitstream/1783.1-7003/1/th_redirect.html.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Ke Y. Study of NY-ESO-1 specific cytotoxic T lymphocytes ex vivo. [Thesis]. Hong Kong University of Science and Technology; 2010. Available from: https://doi.org/10.14711/thesis-b1115986 ; http://repository.ust.hk/ir/bitstream/1783.1-7003/1/th_redirect.html

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Hong Kong University of Science and Technology

11. Fan, Xueling. Study of interleukin-18 in the generation of activated MAGE-A3-specific cytotoxic T lymphocytes in vitro.

Degree: 2010, Hong Kong University of Science and Technology

URL: https://doi.org/10.14711/thesis-b1114683 ; http://lbsearch.ust.hk:3210/sfx?url_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rfr_id=info:sid/HKUST:SPI&rft.genre=article&rft.issn=&rft.volume=&rft.issue=&rft.date=2010&rft.spage=&rft.aulast=Fan&rft.aufirst=Xueling&rft.atitle=Study%20of%20interleukin-18%20in%20the%20generation%20of%20activated%20MAGE-A3-specific%20cytotoxic%20T%20lymphocytes%20in%20vitro&rft.title= ; http://repository.ust.hk/ir/bitstream/1783.1-7173/1/th_redirect.html

► Adoptive T cell transfer involves the administration of tumor-specific cytotoxic T lymphocytes (CTLs) into the patient to kill cancer cells. However, some limitations have restricted… (more)

▼ Adoptive T cell transfer involves the administration of tumor-specific cytotoxic T lymphocytes (CTLs) into the patient to kill cancer cells. However, some limitations have restricted the generation of activated CTLs for clinical application. To overcome these limitations and generate CTLs with higher activation level for use in adoptive T cell transfer, it is necessary to understand how the activation of CTLs is regulated by the cytokines. Interleukin-18 (IL-18) is an interferon-γ (IFN-γ) inducing factor that has an important functional role in regulating CTLs. To generate tumor-specific CTLs, MAGE-A3 is used as a tumor marker, because it is an immunogenic melanoma antigen which belongs to the family of cancer testis antigens, and is widely expressed in many tumor cells. Dendritic cells (DCs), which are the most efficient antigen presenting cells, can present these antigens to naïve T cells. DCs possess the unique ability to activate naïve T cells, causing their differentiation into CTLs, which can kill tumor cells directly. Here, we attempt to use a DC-mediated system supplemented with a recombinant adenovirus (serotype 5) encoding IL-18 (rAd/IL-18) to produce DCs which can secrete HA-tagged IL-18 protein, so as to enhance the generation of activated MAGE-A3-specific CTLs in vitro. A human IL-18 gene was fused with an immunoglobulin lambda light chain leading sequence which leads to the secretion of IL-18 protein from mammalian cells. The recombinant adenovirus encoding IL-18 (rAd/IL-18) was produced by transfecting AD-293 cells with pAd/IL-18. To generate CTLs from naïve T cells, peripheral blood lymphocytes (PBLs) isolated from PBMC were primed with mature DCs, which were engineered to secret HA-tagged IL-18 protein by infection with rAd/IL-18. In Delfia EuTDA cytotoxicity assay, the cytotoxicity of MAGE-A3-specific CTLs was increased in the group in which rAd/IL-18 was loaded into the DC culture compared with the control group in which empty rAd was added. Furthermore, results of FACS analysis showed that the proportion of activated CTLs (as indicated by IFN-γ/CD8+ double-positive cells) in the total number of T cells was increased in the group in which rAd/IL-18 was loaded compared with the empty rAd control group. In conclusion, my data suggests that IL-18 significantly increases the cytotoxic efficacy of CTLs against MAGE-A3-expressing cancer cells, by inducing the activation of MAGE-A3-specific CTLs in a DC-mediated in vitro system, and it also enhances the proliferation of PBLs, which can be used in adoptive T cell transfer for cancer therapy.

Subjects/Keywords: Lymphocytes – Therapeutic use; Interleukins

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Fan, X. (2010). Study of interleukin-18 in the generation of activated MAGE-A3-specific cytotoxic T lymphocytes in vitro. (Thesis). Hong Kong University of Science and Technology. Retrieved from https://doi.org/10.14711/thesis-b1114683 ; http://lbsearch.ust.hk:3210/sfx?url_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rfr_id=info:sid/HKUST:SPI&rft.genre=article&rft.issn=&rft.volume=&rft.issue=&rft.date=2010&rft.spage=&rft.aulast=Fan&rft.aufirst=Xueling&rft.atitle=Study%20of%20interleukin-18%20in%20the%20generation%20of%20activated%20MAGE-A3-specific%20cytotoxic%20T%20lymphocytes%20in%20vitro&rft.title= ; http://repository.ust.hk/ir/bitstream/1783.1-7173/1/th_redirect.html

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Fan, Xueling. “Study of interleukin-18 in the generation of activated MAGE-A3-specific cytotoxic T lymphocytes in vitro.” 2010. Thesis, Hong Kong University of Science and Technology. Accessed June 24, 2019. https://doi.org/10.14711/thesis-b1114683 ; http://lbsearch.ust.hk:3210/sfx?url_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rfr_id=info:sid/HKUST:SPI&rft.genre=article&rft.issn=&rft.volume=&rft.issue=&rft.date=2010&rft.spage=&rft.aulast=Fan&rft.aufirst=Xueling&rft.atitle=Study%20of%20interleukin-18%20in%20the%20generation%20of%20activated%20MAGE-A3-specific%20cytotoxic%20T%20lymphocytes%20in%20vitro&rft.title= ; http://repository.ust.hk/ir/bitstream/1783.1-7173/1/th_redirect.html.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Fan, Xueling. “Study of interleukin-18 in the generation of activated MAGE-A3-specific cytotoxic T lymphocytes in vitro.” 2010. Web. 24 Jun 2019.

Vancouver:

Fan X. Study of interleukin-18 in the generation of activated MAGE-A3-specific cytotoxic T lymphocytes in vitro. [Internet] [Thesis]. Hong Kong University of Science and Technology; 2010. [cited 2019 Jun 24]. Available from: https://doi.org/10.14711/thesis-b1114683 ; http://lbsearch.ust.hk:3210/sfx?url_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rfr_id=info:sid/HKUST:SPI&rft.genre=article&rft.issn=&rft.volume=&rft.issue=&rft.date=2010&rft.spage=&rft.aulast=Fan&rft.aufirst=Xueling&rft.atitle=Study%20of%20interleukin-18%20in%20the%20generation%20of%20activated%20MAGE-A3-specific%20cytotoxic%20T%20lymphocytes%20in%20vitro&rft.title= ; http://repository.ust.hk/ir/bitstream/1783.1-7173/1/th_redirect.html.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Fan X. Study of interleukin-18 in the generation of activated MAGE-A3-specific cytotoxic T lymphocytes in vitro. [Thesis]. Hong Kong University of Science and Technology; 2010. Available from: https://doi.org/10.14711/thesis-b1114683 ; http://lbsearch.ust.hk:3210/sfx?url_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rfr_id=info:sid/HKUST:SPI&rft.genre=article&rft.issn=&rft.volume=&rft.issue=&rft.date=2010&rft.spage=&rft.aulast=Fan&rft.aufirst=Xueling&rft.atitle=Study%20of%20interleukin-18%20in%20the%20generation%20of%20activated%20MAGE-A3-specific%20cytotoxic%20T%20lymphocytes%20in%20vitro&rft.title= ; http://repository.ust.hk/ir/bitstream/1783.1-7173/1/th_redirect.html

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

12. Ghannam, Soufiane. Les lymphocytes Th17 humains : modulation de leur fonction effectrice par les cellules souches mésenchymateuses et caractérisation de leurs propriétés migratoires : Human Th17 lymphocytes : modulation of their effector function by mesenchymal stem cells and characterization of their migratory properties.

Degree: Docteur es, Biologie cellulaire, 2010, Université Montpellier I

URL: http://www.theses.fr/2010MON1T023

►

Les lymphocytes Th17 forment une population de cellules T CD4+ pro-inflammatoires, impliqués non seulement dans l'élimination d'agents pathogènes, mais ayant aussi un rôle délétère dans… (more)

▼

Les lymphocytes Th17 forment une population de cellules T CD4+ pro-inflammatoires, impliqués non seulement dans l'élimination d'agents pathogènes, mais ayant aussi un rôle délétère dans l'induction de maladies inflammatoires chroniques. Ils expriment spécifiquement le récepteur de chimiokines CCR6, qui a pour ligand le CCL20 mais aussi les β-defensine-1, 2 et 3, peptides ayant une activité antimicrobienne. Les cellules souches mésenchymateuses (CSMs) représentent une population cellulaire hétérogène exerçant diverses propriétés immunomodulatrices.Les résultats obtenus dans ce travail de thèse montrent que l'environnement inflammatoire contribue à augmenter l'adhésion des lymphocytes Th17 aux CSMs, et qu'elle est régulée par l'interaction du CCR6 avec ses ligands ; que les CSMs exercent, en partie via la sécrétion de PGE2, des effets anti-inflammatoires en faisant acquérir un phénotype régulateur aux lymphocytes Th17 différenciés, soulignant ainsi la plasticité de ces derniers.De plus, nous avons montré que les lymphocytes Th17 activés par l'antigène produisent du CCL20 et induisent, via la production de l'IL-17 et de l'IL-22, la sécrétion d'hBD-2, mais pas celle des hBD-1 et 3, par des kératinocytes épidermiques humains et de la peau reconstituée; que le CCL20, ainsi que la hBD-2, induisent l'arrêt de ces cellules sur l'endothélium enflammé in vitro en conditions de cisaillement. Finalement, l'activation spécifique d'antigène des lymphocytes Th17 entraîne une perte de l'expression de CCR6, ce qui provoque ainsi un état transitoire de non réponse à une nouvelle stimulation de ces cellules avec les ligands de CCR6, permettant leur migration ultérieure hors du tissu enflammé.

Th17 cells form a population CD4+ T cells with strong pro-inflammatory properties that are not only involved in the clearance of pathogens, but also play a deleterious role of in the pathogenesis of inflammatory disease. Th17 cells specifically express CCR6, a chemokine receptor that binds to its unique chemokine ligand, CCL20, as well as to human β-defensin (hBD)-1, 2 and 3, peptides with anti-microbial activity. Mesenchymal stem cells (MSC) represent a heterogenous population that exert broad immunomodulatory effects.The results from the studies carried out during this thesis show that the inflammatory environment contributes to increased adhesion of Th17 cells to MSCs, which is mediated via the interaction of CCR6 with its ligands, and that MSCs exert, in part via the secretion of PGE2, anti-inflammatory effects through the induction of a T regulatory cell phenotype in fully differentiated tissue-infiltrating Th17 cells, thereby underscoring the plasticity of the latter cells.Furthermore, the results show that antigen-activated Th17 cells produce CCL20 and induce, via the production of both IL-17 and IL-22, the secretion of hBD-2, but not 1 and 3, by normal human epidermal keratinocytes and reconstituted skin, and that CCL20, as well as hBD-2, induce arrest of these cells onto inflamed…

Advisors/Committee Members: Yssel, Hans (thesis director).

Subjects/Keywords: Cellules souches mésenchymateuses; Lymphocytes Th17; Lymphocytes T régulateurs; Inflammation; Mesenchymal stem cells; Th17 lymphocytes; T regulatory lymphocytes; Inflamamation

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Ghannam, S. (2010). Les lymphocytes Th17 humains : modulation de leur fonction effectrice par les cellules souches mésenchymateuses et caractérisation de leurs propriétés migratoires : Human Th17 lymphocytes : modulation of their effector function by mesenchymal stem cells and characterization of their migratory properties. (Doctoral Dissertation). Université Montpellier I. Retrieved from http://www.theses.fr/2010MON1T023

Chicago Manual of Style (16^th Edition):

Ghannam, Soufiane. “Les lymphocytes Th17 humains : modulation de leur fonction effectrice par les cellules souches mésenchymateuses et caractérisation de leurs propriétés migratoires : Human Th17 lymphocytes : modulation of their effector function by mesenchymal stem cells and characterization of their migratory properties.” 2010. Doctoral Dissertation, Université Montpellier I. Accessed June 24, 2019. http://www.theses.fr/2010MON1T023.

MLA Handbook (7^th Edition):

Ghannam, Soufiane. “Les lymphocytes Th17 humains : modulation de leur fonction effectrice par les cellules souches mésenchymateuses et caractérisation de leurs propriétés migratoires : Human Th17 lymphocytes : modulation of their effector function by mesenchymal stem cells and characterization of their migratory properties.” 2010. Web. 24 Jun 2019.

Vancouver:

Ghannam S. Les lymphocytes Th17 humains : modulation de leur fonction effectrice par les cellules souches mésenchymateuses et caractérisation de leurs propriétés migratoires : Human Th17 lymphocytes : modulation of their effector function by mesenchymal stem cells and characterization of their migratory properties. [Internet] [Doctoral dissertation]. Université Montpellier I; 2010. [cited 2019 Jun 24]. Available from: http://www.theses.fr/2010MON1T023.

Council of Science Editors:

Ghannam S. Les lymphocytes Th17 humains : modulation de leur fonction effectrice par les cellules souches mésenchymateuses et caractérisation de leurs propriétés migratoires : Human Th17 lymphocytes : modulation of their effector function by mesenchymal stem cells and characterization of their migratory properties. [Doctoral Dissertation]. Université Montpellier I; 2010. Available from: http://www.theses.fr/2010MON1T023

Université de Sherbrooke

13. Fock-Chow-Tho, David. Développement de stratégies et d'outils analytiques novateurs pour la détection de vaches atteintes de paratuberculose .

Degree: 2016, Université de Sherbrooke

URL: http://hdl.handle.net/11143/9774

► La paratuberculose bovine est une maladie causée par la mycobactérie Mycobacterium avium subsp. paratuberculosis (MAP). Elle est responsable d’énormes pertes économiques dans le monde. En… (more)

▼ La paratuberculose bovine est une maladie causée par la mycobactérie Mycobacterium avium subsp. paratuberculosis (MAP). Elle est responsable d’énormes pertes économiques dans le monde. En effet, cette maladie provoque la diminution de la production laitière chez les vaches ainsi qu’un état de fatigue général en raison d’une entérite chronique chez les sujets atteints. Des indices mettent également en évidence le potentiel zoonotique de MAP et malheureusement aucun traitement n’est connu à ce jour. Ainsi, les stratégies actuelles pour contrer la maladie dans les troupeaux reposent plutôt sur des actions préventives. Actuellement, le diagnostic de la paratuberculose reste difficile et la prévalence de la maladie est probablement sous-estimée dans les troupeaux en raison de la faible sensibilité des tests diagnostiques. Dans l’optique de développer une nouvelle méthode diagnostique pour la paratuberculose, ce projet de recherche s’est établi en deux étapes : la mise en place d’un système de diagnostic robuste des animaux par les méthodes de dépistage traditionnelles, incluant une étude comparative de l’efficacité de trousses commerciales, et finalement l’étude de la prolifération cellulaire spécifique des lymphocytes comme d’une épreuve diagnostique pour la maladie. Ainsi, la comparaison des trousses commerciales a démontré un écart d’efficacité qui a permis d’établir de nouvelles recommandations pour l’analyse des animaux ainsi que l’amélioration du diagnostic de la paratuberculose dans les troupeaux. Concernant le développement d’une méthode de diagnostique basée sur la prolifération lymphocytaire, de nombreuses difficultés techniques ont entravé le bon déroulement du projet mais ces travaux montrent la possibilité de caractériser de manière plus complète les différents types lymphocytaires par cytométrie de flux chez le bovin laitier. Advisors/Committee Members: Bissonnette, Nathalie (advisor), Steimle, Viktor (advisor).

Subjects/Keywords: Paratuberculose bovine; Lymphocytes; Prolifération; Diagnostic

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Fock-Chow-Tho, D. (2016). Développement de stratégies et d'outils analytiques novateurs pour la détection de vaches atteintes de paratuberculose . (Masters Thesis). Université de Sherbrooke. Retrieved from http://hdl.handle.net/11143/9774

Chicago Manual of Style (16^th Edition):

Fock-Chow-Tho, David. “Développement de stratégies et d'outils analytiques novateurs pour la détection de vaches atteintes de paratuberculose .” 2016. Masters Thesis, Université de Sherbrooke. Accessed June 24, 2019. http://hdl.handle.net/11143/9774.

MLA Handbook (7^th Edition):

Fock-Chow-Tho, David. “Développement de stratégies et d'outils analytiques novateurs pour la détection de vaches atteintes de paratuberculose .” 2016. Web. 24 Jun 2019.

Vancouver:

Fock-Chow-Tho D. Développement de stratégies et d'outils analytiques novateurs pour la détection de vaches atteintes de paratuberculose . [Internet] [Masters thesis]. Université de Sherbrooke; 2016. [cited 2019 Jun 24]. Available from: http://hdl.handle.net/11143/9774.

Council of Science Editors:

Fock-Chow-Tho D. Développement de stratégies et d'outils analytiques novateurs pour la détection de vaches atteintes de paratuberculose . [Masters Thesis]. Université de Sherbrooke; 2016. Available from: http://hdl.handle.net/11143/9774

University of British Columbia

14. Wong, S. Y. Comparison between locomotory behavior of lymphocytes of WWV mutant and normal house-mouse .

Degree: 1969, University of British Columbia

URL: http://hdl.handle.net/2429/35299

► The rate of motility of lymphocytes from anemic mice of the WW[superscript V] genotype and their normal ++ littermates was determined in vitro by time-lapse… (more)

Subjects/Keywords: Lymphocytes; Mice

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Wong, S. Y. (1969). Comparison between locomotory behavior of lymphocytes of WWV mutant and normal house-mouse . (Thesis). University of British Columbia. Retrieved from http://hdl.handle.net/2429/35299

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Wong, S Y. “Comparison between locomotory behavior of lymphocytes of WWV mutant and normal house-mouse .” 1969. Thesis, University of British Columbia. Accessed June 24, 2019. http://hdl.handle.net/2429/35299.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Wong, S Y. “Comparison between locomotory behavior of lymphocytes of WWV mutant and normal house-mouse .” 1969. Web. 24 Jun 2019.

Vancouver:

Wong SY. Comparison between locomotory behavior of lymphocytes of WWV mutant and normal house-mouse . [Internet] [Thesis]. University of British Columbia; 1969. [cited 2019 Jun 24]. Available from: http://hdl.handle.net/2429/35299.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Wong SY. Comparison between locomotory behavior of lymphocytes of WWV mutant and normal house-mouse . [Thesis]. University of British Columbia; 1969. Available from: http://hdl.handle.net/2429/35299

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Cornell University

15. Prieto, Jennifer. Equine B Lymphopoiesis And Cd5+ B Lymphocytes .

Degree: 2015, Cornell University

URL: http://hdl.handle.net/1813/41107

► The development of the humoral immune system starts during gestation with the production of two major types of B cells: B1 and B2. B2 cells… (more)

▼ The development of the humoral immune system starts during gestation with the production of two major types of B cells: B1 and B2. B2 cells are the classic players in the adaptive humoral immunity, while B1 cells are considered components of innate immunity and have important developmental, phenotypic, and functional differences with B2 cells. B1 cells are predominant early in ontology in mice and humans; therefore, my studies of B cell development in the horse began in the primary lymphoid organs of the fetus. In the first part of my study, I tested how primary lymphoid tissues of the equine fetus were equipped to support B cell hematopoiesis and immunoglobulin (Ig) diversity of the pre-immune repertoire. I found that the equine fetal liver and the bone marrow are active sites of hematopoiesis based on the expression of signature mRNA (c-KIT, CD34, IL7R, CXCL12, IRF8, PU.1, PAX5, NOTCH1, GATA1, CEBPA) and protein markers (CD34, CD19, IgM, CD3, CD4, CD5, CD8, CD11b, CD172A) of hematopoietic development and leukocyte differentiation molecules, respectively at approximately 100 days of gestation (DG). Immunoglobulin V(D)J segments were sequenced in primary lymphoid organs of the equine fetus and adult horse, revealing that some aspects of Ig diversity in the adult were established in the fetus (similar heavy chain VDJ segment utilization, CDR3 lengths), while others were developmentally programmed (lambda light chain segment utilization, sequence diversity). These data suggest that the B cells produced in the liver and bone marrow of the equine fetus generate a wide repertoire of pre-immune Igs for protection, and the more diverse use of different lambda variable gene segments in fetal life may provide the neonate an opportunity to respond to a wider range of antigens at birth. In the second part of my study, I tested aspects of ontogeny, phenotype, and function of equine CD5hi B cells, putative B1-like cells. The expression of CD5 can distinguish murine B1a from B2 cells, and my study tested how this marker could distinguish B1-like cells in the horse. I learned that CD5hi B cells were more frequent early in development and in the peritoneal cavity. CD5hi cells more frequently coexpressed CD2, CD21, and CD11b than CD5lo B cells in the peripheral blood when measured with flow cytometry, but had similar mRNA expression of B1 and B2 signature genes including DGKA, FGL2, PAX5, and IGHM when measured with qRTPCR. Sequencing of lambda light chain segments revealed that CD5hi and CD5lo B cells generate similar levels of immunoglobulin diversity; however, CD5hi B cells more frequently bound to fluorescence-labeled phosphorylcholine and secreted IL-10 in vitro. CD5hi B cells also represented a greater proportion of remaining B cells in horses with common variable immunodeficiency. Taken together, the results of my study show that equine CD5hi B cells share some of the same characteristics attributed to B1 cells in other species, and may be explored for immune protection in patients with humoral immunodeficiency. Advisors/Committee Members: Appleton,Judith Ann (committeeMember), Marquis,Helene (committeeMember), Schimenti,John C. (committeeMember), Fortier,Lisa Ann (committeeMember).

Subjects/Keywords: Equine; B Lymphocytes; Development

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Prieto, J. (2015). Equine B Lymphopoiesis And Cd5+ B Lymphocytes . (Thesis). Cornell University. Retrieved from http://hdl.handle.net/1813/41107

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Prieto, Jennifer. “Equine B Lymphopoiesis And Cd5+ B Lymphocytes .” 2015. Thesis, Cornell University. Accessed June 24, 2019. http://hdl.handle.net/1813/41107.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Prieto, Jennifer. “Equine B Lymphopoiesis And Cd5+ B Lymphocytes .” 2015. Web. 24 Jun 2019.

Vancouver:

Prieto J. Equine B Lymphopoiesis And Cd5+ B Lymphocytes . [Internet] [Thesis]. Cornell University; 2015. [cited 2019 Jun 24]. Available from: http://hdl.handle.net/1813/41107.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Prieto J. Equine B Lymphopoiesis And Cd5+ B Lymphocytes . [Thesis]. Cornell University; 2015. Available from: http://hdl.handle.net/1813/41107

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Université Laval

16. Guérard, Simon. Kératinocytes : cellules instigatrices de l'inflammation dans le psoriasis : étude sur la capacité des kératinocytes psoriasiques à activer in vitro les lymphocytes T et les neutrophiles.

Degree: 2011, Université Laval

URL: http://hdl.handle.net/20.500.11794/23652

► Le rôle primordial des cellules de la peau dans la réponse immunitaire est désormais bien établi. Cependant, pourraient-elles également être responsables de l'activation du système… (more)

Subjects/Keywords: Kératinocytes; Psoriasis; Lymphocytes T; Neutrophiles

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APA (6^th Edition):

Guérard, S. (2011). Kératinocytes : cellules instigatrices de l'inflammation dans le psoriasis : étude sur la capacité des kératinocytes psoriasiques à activer in vitro les lymphocytes T et les neutrophiles. (Thesis). Université Laval. Retrieved from http://hdl.handle.net/20.500.11794/23652

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Guérard, Simon. “Kératinocytes : cellules instigatrices de l'inflammation dans le psoriasis : étude sur la capacité des kératinocytes psoriasiques à activer in vitro les lymphocytes T et les neutrophiles.” 2011. Thesis, Université Laval. Accessed June 24, 2019. http://hdl.handle.net/20.500.11794/23652.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Guérard, Simon. “Kératinocytes : cellules instigatrices de l'inflammation dans le psoriasis : étude sur la capacité des kératinocytes psoriasiques à activer in vitro les lymphocytes T et les neutrophiles.” 2011. Web. 24 Jun 2019.

Vancouver:

Guérard S. Kératinocytes : cellules instigatrices de l'inflammation dans le psoriasis : étude sur la capacité des kératinocytes psoriasiques à activer in vitro les lymphocytes T et les neutrophiles. [Internet] [Thesis]. Université Laval; 2011. [cited 2019 Jun 24]. Available from: http://hdl.handle.net/20.500.11794/23652.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Guérard S. Kératinocytes : cellules instigatrices de l'inflammation dans le psoriasis : étude sur la capacité des kératinocytes psoriasiques à activer in vitro les lymphocytes T et les neutrophiles. [Thesis]. Université Laval; 2011. Available from: http://hdl.handle.net/20.500.11794/23652

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Université Laval

17. Gervais-St-Amour, Catherine. Étude de la différenciation des lymphocytes B mémoires en milieu sans sérum.

Degree: 2013, Université Laval

URL: http://hdl.handle.net/20.500.11794/24508

► Les infections opportunistes sont l’une des principales causes de mortalité associées à la greffe de cellules souches. Afin d’aider à reconstituer le système immunitaire des… (more)

Subjects/Keywords: Lymphocytes B – Différenciation; Plasmocytes

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APA (6^th Edition):

Gervais-St-Amour, C. (2013). Étude de la différenciation des lymphocytes B mémoires en milieu sans sérum. (Thesis). Université Laval. Retrieved from http://hdl.handle.net/20.500.11794/24508

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Gervais-St-Amour, Catherine. “Étude de la différenciation des lymphocytes B mémoires en milieu sans sérum.” 2013. Thesis, Université Laval. Accessed June 24, 2019. http://hdl.handle.net/20.500.11794/24508.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Gervais-St-Amour, Catherine. “Étude de la différenciation des lymphocytes B mémoires en milieu sans sérum.” 2013. Web. 24 Jun 2019.

Vancouver:

Gervais-St-Amour C. Étude de la différenciation des lymphocytes B mémoires en milieu sans sérum. [Internet] [Thesis]. Université Laval; 2013. [cited 2019 Jun 24]. Available from: http://hdl.handle.net/20.500.11794/24508.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Gervais-St-Amour C. Étude de la différenciation des lymphocytes B mémoires en milieu sans sérum. [Thesis]. Université Laval; 2013. Available from: http://hdl.handle.net/20.500.11794/24508

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Université Laval

18. Martin, Guillaume. Impact fonctionnel de l'interaction kératinocyte-lymphocyte T au cours du psoriasis.

Degree: 2011, Université Laval

URL: http://hdl.handle.net/20.500.11794/22776

► Un important réseau de cytokines serait en grande partie responsable de la formation des lésions psoriasiques. La présence de cellules immunitaires dans des biopsies de… (more)

Subjects/Keywords: Psoriasis; Lymphocytes T; Kératinocytes

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APA (6^th Edition):

Martin, G. (2011). Impact fonctionnel de l'interaction kératinocyte-lymphocyte T au cours du psoriasis. (Thesis). Université Laval. Retrieved from http://hdl.handle.net/20.500.11794/22776

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Martin, Guillaume. “Impact fonctionnel de l'interaction kératinocyte-lymphocyte T au cours du psoriasis.” 2011. Thesis, Université Laval. Accessed June 24, 2019. http://hdl.handle.net/20.500.11794/22776.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Martin, Guillaume. “Impact fonctionnel de l'interaction kératinocyte-lymphocyte T au cours du psoriasis.” 2011. Web. 24 Jun 2019.

Vancouver:

Martin G. Impact fonctionnel de l'interaction kératinocyte-lymphocyte T au cours du psoriasis. [Internet] [Thesis]. Université Laval; 2011. [cited 2019 Jun 24]. Available from: http://hdl.handle.net/20.500.11794/22776.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Martin G. Impact fonctionnel de l'interaction kératinocyte-lymphocyte T au cours du psoriasis. [Thesis]. Université Laval; 2011. Available from: http://hdl.handle.net/20.500.11794/22776

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Université Laval

19. Bonnaure, Guillaume. Les plasmocytes et leur niche : étude de la génération de plasmocytes humains in vitro.

Degree: 2016, Université Laval

URL: http://hdl.handle.net/20.500.11794/27053

►

Tableau d'honneur de la Faculté des études supérieures et postdoctorales, 2016-2017

Chez l’humain, les lymphocytes B mémoires IgG+ et IgA+ sont des cellules clés de… (more)

▼

Tableau d'honneur de la Faculté des études supérieures et postdoctorales, 2016-2017

Chez l’humain, les lymphocytes B mémoires IgG+ et IgA+ sont des cellules clés de l’immunité humorale. Ces cellules mémoires sont maintenues à long-terme dans notre organisme. Elles représentent une défense rapide et efficace contre toutes les infections que nous avons déjà vaincues pendant notre vie. Ces cellules mémoires qui rencontrent à nouveau leur antigène se différencient rapidement en plasmocytes à courte vie, et permettent la sécrétion massive d’immunoglobuline (Ig). La contrepartie mémoire de ces cellules sont les plasmocytes à longue vie qui sont présents dans les niches de la moelle osseuse et y sécrètent en permanence des anticorps protecteurs qui circulent dans le sang. Ces cellules sécrétrices peuvent avoir une durée de vie allant de dizaines d’années à la vie entière de l’individu. Les patients qui reçoivent des traitements de chimiothérapie ou de radiothérapie sont privés de ces cellules mémoires détruites par ces traitements au même titre que les cellules cancéreuses. Ces patients deviennent vulnérables aux infections et leur survie dépend de la régénération rapide de leur système hématopoïétique. Notre équipe a déjà mis au point une méthode pour préparer de grandes quantités des cellules mémoires capables de sécréter des IgG et des IgA. Les présents travaux visent à générer des plasmocytes fonctionnels et capables de survivre à long terme in vitro. La stratégie expérimentale visait à établir des conditions permettant de se rapprocher de l’environnement de la moelle osseuse. Dans un premier temps, nous avons étudié les paramètres permettant la différenciation des lymphocytes B mémoires en plasmocytes. Étant donné l’importance du potentiel redox dans l’environnement de la moelle osseuse, nous avons d’abord tenté d’en contrôler l’impact avec un antioxydant, le N-acétyle cystéine (NAC). Nos résultats ont démontré que le NAC avait un effet significatif et diminuait la phosphorylation de la protéine STAT3 en raison d’une inhibition des kinases JAK2 et JAK3. Étonnamment, cet antioxydant retardait la différenciation de nos lymphocytes B qui étaient stimulés avec une forte interaction CD40-CD154. Par la suite, la comparaison des interactions CD40-CD154 et CD27-CD70 a permis de conclure qu’il était essentiel de réduire à son minimum l’interaction CD40-CD154 et qu’il fallait ajouter les cytokines IL-6 et IL-10. Les cellules CD31+CD38+CD138+ générées présentaient un phénotype similaire à celui des plasmocytes de la moelle osseuse. Malheureusement la fréquence de ces cellules était faible et leur viabilité insuffisante. Afin d’augmenter la survie de ces cellules le dernier volet de nos travaux visait à se rapprocher des niches de la moelle osseuse. Notre but a été atteint en ajoutant des cellules mésenchymateuses issues de la moelle osseuse en présence de 8% de dioxygène (O2). Les cellules CD31+CD38+CD138+ générées ont une excellente viabilité et représentent plus de 50% des cellules totales en culture. De plus, le modèle de…

Advisors/Committee Members: Darveau, André, Néron, Sonia.

Subjects/Keywords: Plasmocytes; Reproduction; Lymphocytes B

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APA (6^th Edition):

Bonnaure, G. (2016). Les plasmocytes et leur niche : étude de la génération de plasmocytes humains in vitro. (Thesis). Université Laval. Retrieved from http://hdl.handle.net/20.500.11794/27053

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Bonnaure, Guillaume. “Les plasmocytes et leur niche : étude de la génération de plasmocytes humains in vitro.” 2016. Thesis, Université Laval. Accessed June 24, 2019. http://hdl.handle.net/20.500.11794/27053.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Bonnaure, Guillaume. “Les plasmocytes et leur niche : étude de la génération de plasmocytes humains in vitro.” 2016. Web. 24 Jun 2019.

Vancouver:

Bonnaure G. Les plasmocytes et leur niche : étude de la génération de plasmocytes humains in vitro. [Internet] [Thesis]. Université Laval; 2016. [cited 2019 Jun 24]. Available from: http://hdl.handle.net/20.500.11794/27053.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Bonnaure G. Les plasmocytes et leur niche : étude de la génération de plasmocytes humains in vitro. [Thesis]. Université Laval; 2016. Available from: http://hdl.handle.net/20.500.11794/27053

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Michigan State University

20. Chang, Hsun-Lang. The developmental expression of CD45 glycoprotein in murine t lymphocytes.

Degree: PhD, Department of Microbiology, 1990, Michigan State University

URL: http://etd.lib.msu.edu/islandora/object/etd:29092

Subjects/Keywords: Glycoproteins; Lymphocytes

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APA (6^th Edition):

Chang, H. (1990). The developmental expression of CD45 glycoprotein in murine t lymphocytes. (Doctoral Dissertation). Michigan State University. Retrieved from http://etd.lib.msu.edu/islandora/object/etd:29092

Chicago Manual of Style (16^th Edition):

Chang, Hsun-Lang. “The developmental expression of CD45 glycoprotein in murine t lymphocytes.” 1990. Doctoral Dissertation, Michigan State University. Accessed June 24, 2019. http://etd.lib.msu.edu/islandora/object/etd:29092.

MLA Handbook (7^th Edition):

Chang, Hsun-Lang. “The developmental expression of CD45 glycoprotein in murine t lymphocytes.” 1990. Web. 24 Jun 2019.

Vancouver:

Chang H. The developmental expression of CD45 glycoprotein in murine t lymphocytes. [Internet] [Doctoral dissertation]. Michigan State University; 1990. [cited 2019 Jun 24]. Available from: http://etd.lib.msu.edu/islandora/object/etd:29092.

Council of Science Editors:

Chang H. The developmental expression of CD45 glycoprotein in murine t lymphocytes. [Doctoral Dissertation]. Michigan State University; 1990. Available from: http://etd.lib.msu.edu/islandora/object/etd:29092

University of Toronto

21. Guerra, Fiona. The Role of Homeodomain-interacting Protein Kinase (HIPK)-1 in B Lymphocytes.

Degree: 2011, University of Toronto

URL: http://hdl.handle.net/1807/29736

►

The homeodomain-interacting protein kinase (HIPK) family is comprised of four evolutionarily conserved and highly related serine/threonine kinases originally identified as co-repressors for homeodomain-containing transcription factors.… (more)

Subjects/Keywords: immunology; B lymphocytes; 0982

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APA (6^th Edition):

Guerra, F. (2011). The Role of Homeodomain-interacting Protein Kinase (HIPK)-1 in B Lymphocytes. (Doctoral Dissertation). University of Toronto. Retrieved from http://hdl.handle.net/1807/29736

Chicago Manual of Style (16^th Edition):

Guerra, Fiona. “The Role of Homeodomain-interacting Protein Kinase (HIPK)-1 in B Lymphocytes.” 2011. Doctoral Dissertation, University of Toronto. Accessed June 24, 2019. http://hdl.handle.net/1807/29736.

MLA Handbook (7^th Edition):

Guerra, Fiona. “The Role of Homeodomain-interacting Protein Kinase (HIPK)-1 in B Lymphocytes.” 2011. Web. 24 Jun 2019.

Vancouver:

Guerra F. The Role of Homeodomain-interacting Protein Kinase (HIPK)-1 in B Lymphocytes. [Internet] [Doctoral dissertation]. University of Toronto; 2011. [cited 2019 Jun 24]. Available from: http://hdl.handle.net/1807/29736.

Council of Science Editors:

Guerra F. The Role of Homeodomain-interacting Protein Kinase (HIPK)-1 in B Lymphocytes. [Doctoral Dissertation]. University of Toronto; 2011. Available from: http://hdl.handle.net/1807/29736

University of Arizona

22. Endres, Robert Otto, 1949-. INVESTIGATION OF THE ROLE OF LYMPHOCYTE ECTOGLYCOSYLTRANSFERASES IN BLASTOGENESIS AND CELLULAR INTERACTIONS .

Degree: 1976, University of Arizona

URL: http://hdl.handle.net/10150/289413

Subjects/Keywords: Lymphocytes.; Ectoglycosyltransferase.

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APA (6^th Edition):

Endres, Robert Otto, 1. (1976). INVESTIGATION OF THE ROLE OF LYMPHOCYTE ECTOGLYCOSYLTRANSFERASES IN BLASTOGENESIS AND CELLULAR INTERACTIONS . (Doctoral Dissertation). University of Arizona. Retrieved from http://hdl.handle.net/10150/289413

Chicago Manual of Style (16^th Edition):

Endres, Robert Otto, 1949-. “INVESTIGATION OF THE ROLE OF LYMPHOCYTE ECTOGLYCOSYLTRANSFERASES IN BLASTOGENESIS AND CELLULAR INTERACTIONS .” 1976. Doctoral Dissertation, University of Arizona. Accessed June 24, 2019. http://hdl.handle.net/10150/289413.

MLA Handbook (7^th Edition):

Endres, Robert Otto, 1949-. “INVESTIGATION OF THE ROLE OF LYMPHOCYTE ECTOGLYCOSYLTRANSFERASES IN BLASTOGENESIS AND CELLULAR INTERACTIONS .” 1976. Web. 24 Jun 2019.

Vancouver:

Endres, Robert Otto 1. INVESTIGATION OF THE ROLE OF LYMPHOCYTE ECTOGLYCOSYLTRANSFERASES IN BLASTOGENESIS AND CELLULAR INTERACTIONS . [Internet] [Doctoral dissertation]. University of Arizona; 1976. [cited 2019 Jun 24]. Available from: http://hdl.handle.net/10150/289413.

Council of Science Editors:

Endres, Robert Otto 1. INVESTIGATION OF THE ROLE OF LYMPHOCYTE ECTOGLYCOSYLTRANSFERASES IN BLASTOGENESIS AND CELLULAR INTERACTIONS . [Doctoral Dissertation]. University of Arizona; 1976. Available from: http://hdl.handle.net/10150/289413

University of Texas Southwestern Medical Center

23. Belkaya, Serkan. Characterization of Stress Responsive MicroRNAs and Their Roles in T Cell Development.

Degree: 2014, University of Texas Southwestern Medical Center

URL: http://hdl.handle.net/2152.5/1422

► Physiological stress evokes rapid changes in both the innate and adaptive immune responses. Immature αβ T cells developing in the thymus are particularly sensitive to… (more)

▼ Physiological stress evokes rapid changes in both the innate and adaptive immune responses. Immature αβ T cells developing in the thymus are particularly sensitive to stress, with infections and/or exposure to lipopolysaccharide or glucocorticoids eliciting a rapid apoptotic program. MicroRNAs (miRs) are short, non-coding RNAs that play critical roles in the immune system by targeting diverse mRNAs. We hypothesized that a subset of thymically encoded miRs would be stress responsive and modulate thymopoiesis. Thymic miR profiling revealed 18 distinct miRs that are dysregulated more than 1.5-fold in response to lipopolysaccharide or the synthetic glucocorticoid dexamethasone. These stress-responsive miRs are dynamically regulated in distinct thymocyte subsets. We utilized both transgenic and gene-targeting approaches to study the impact of these miRs on thymopoiesis under normal and stress conditions. MiR-181d is the most down-regulated thymic miR in response to stress. The over-expression of miR-181d in developing thymocytes reduced the number of immature CD4+CD8+ thymocytes. Lipopolysaccharide or dexamethasone injections caused a 4-fold greater loss of these cells than in the wild type controls. The targeted elimination of miR-181d resulted in a thymus stress-responsiveness similar to wild-type mice, suggesting a functional redundancy between miR-181 family members. Gene expression comparisons further indicated that miR-181d affects a number of stress, metabolic, and signaling pathways. These findings demonstrate that selected miRs enhance stress-mediated thymic involution in vivo. MiR-185, another stress-responsive miR in murine thymus, is haploinsufficient in almost all individuals with 22q11.2 deletion/DiGeorge syndrome that can present with immune, cardiac, parathyroid, and psychological problems. The molecular targets of miR-185 in thymocytes are unknown. Transgenic expression of miR-185 attenuated thymopoiesis at the TCRβ-selection checkpoint and during positive selection. This caused a peripheral T cell lymphopenia. Mzb1, NFATc3, and Camk4 were identified as novel miR-185 targets. Elevations in miR-185 enhanced TCR-dependent intracellular calcium levels, while a knockdown of miR-185 diminished these calcium responses. These effects concur with reductions in Mzb1, an endoplasmic reticulum calcium regulator. Consistent with the haploinsufficiency of miR-185, Mzb1 levels were elevated in thymocyte extracts from several 22q11.2 deletion/DiGeorge syndrome patients. These findings indicate that miR-185 regulates T cell development through its targeting of several mRNAs including Mzb1. Advisors/Committee Members: Hooper, Lora V., van Oers, Nicolai S. C., Chen, Zhijian J., Yarovinsky, Felix, Liou, Jen.

Subjects/Keywords: MicroRNAs; Stress, Physiological; T-Lymphocytes

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APA (6^th Edition):

Belkaya, S. (2014). Characterization of Stress Responsive MicroRNAs and Their Roles in T Cell Development. (Thesis). University of Texas Southwestern Medical Center. Retrieved from http://hdl.handle.net/2152.5/1422

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Belkaya, Serkan. “Characterization of Stress Responsive MicroRNAs and Their Roles in T Cell Development.” 2014. Thesis, University of Texas Southwestern Medical Center. Accessed June 24, 2019. http://hdl.handle.net/2152.5/1422.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Belkaya, Serkan. “Characterization of Stress Responsive MicroRNAs and Their Roles in T Cell Development.” 2014. Web. 24 Jun 2019.

Vancouver:

Belkaya S. Characterization of Stress Responsive MicroRNAs and Their Roles in T Cell Development. [Internet] [Thesis]. University of Texas Southwestern Medical Center; 2014. [cited 2019 Jun 24]. Available from: http://hdl.handle.net/2152.5/1422.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Belkaya S. Characterization of Stress Responsive MicroRNAs and Their Roles in T Cell Development. [Thesis]. University of Texas Southwestern Medical Center; 2014. Available from: http://hdl.handle.net/2152.5/1422

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Arizona

24. Kiehn, Timothy Everett, 1939-. COMPUTER ANALYSIS OF TRANSFORMING LYMPHOCYTES .

Degree: 1972, University of Arizona

URL: http://hdl.handle.net/10150/287841

Subjects/Keywords: Lymphocytes – Research.

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APA (6^th Edition):

Kiehn, Timothy Everett, 1. (1972). COMPUTER ANALYSIS OF TRANSFORMING LYMPHOCYTES . (Doctoral Dissertation). University of Arizona. Retrieved from http://hdl.handle.net/10150/287841

Chicago Manual of Style (16^th Edition):

Kiehn, Timothy Everett, 1939-. “COMPUTER ANALYSIS OF TRANSFORMING LYMPHOCYTES .” 1972. Doctoral Dissertation, University of Arizona. Accessed June 24, 2019. http://hdl.handle.net/10150/287841.

MLA Handbook (7^th Edition):

Kiehn, Timothy Everett, 1939-. “COMPUTER ANALYSIS OF TRANSFORMING LYMPHOCYTES .” 1972. Web. 24 Jun 2019.

Vancouver:

Kiehn, Timothy Everett 1. COMPUTER ANALYSIS OF TRANSFORMING LYMPHOCYTES . [Internet] [Doctoral dissertation]. University of Arizona; 1972. [cited 2019 Jun 24]. Available from: http://hdl.handle.net/10150/287841.

Council of Science Editors:

Kiehn, Timothy Everett 1. COMPUTER ANALYSIS OF TRANSFORMING LYMPHOCYTES . [Doctoral Dissertation]. University of Arizona; 1972. Available from: http://hdl.handle.net/10150/287841

University of Arizona

25. Dean, Jack H. STUDIES ON THE INTERACTION OF LYMPHOCYTES WITH POLYRIBOINOSINIC: POLYRIBOCYTIDYLIC ACID; LYMPHOCYTE ACTIVATION AND INTERFERON PRODUCTION .

Degree: 1972, University of Arizona

URL: http://hdl.handle.net/10150/287998

Subjects/Keywords: Interferon.; Lymphocytes.

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APA (6^th Edition):

Dean, J. H. (1972). STUDIES ON THE INTERACTION OF LYMPHOCYTES WITH POLYRIBOINOSINIC: POLYRIBOCYTIDYLIC ACID; LYMPHOCYTE ACTIVATION AND INTERFERON PRODUCTION . (Doctoral Dissertation). University of Arizona. Retrieved from http://hdl.handle.net/10150/287998

Chicago Manual of Style (16^th Edition):

Dean, Jack H. “STUDIES ON THE INTERACTION OF LYMPHOCYTES WITH POLYRIBOINOSINIC: POLYRIBOCYTIDYLIC ACID; LYMPHOCYTE ACTIVATION AND INTERFERON PRODUCTION .” 1972. Doctoral Dissertation, University of Arizona. Accessed June 24, 2019. http://hdl.handle.net/10150/287998.

MLA Handbook (7^th Edition):

Dean, Jack H. “STUDIES ON THE INTERACTION OF LYMPHOCYTES WITH POLYRIBOINOSINIC: POLYRIBOCYTIDYLIC ACID; LYMPHOCYTE ACTIVATION AND INTERFERON PRODUCTION .” 1972. Web. 24 Jun 2019.

Vancouver:

Dean JH. STUDIES ON THE INTERACTION OF LYMPHOCYTES WITH POLYRIBOINOSINIC: POLYRIBOCYTIDYLIC ACID; LYMPHOCYTE ACTIVATION AND INTERFERON PRODUCTION . [Internet] [Doctoral dissertation]. University of Arizona; 1972. [cited 2019 Jun 24]. Available from: http://hdl.handle.net/10150/287998.

Council of Science Editors:

Dean JH. STUDIES ON THE INTERACTION OF LYMPHOCYTES WITH POLYRIBOINOSINIC: POLYRIBOCYTIDYLIC ACID; LYMPHOCYTE ACTIVATION AND INTERFERON PRODUCTION . [Doctoral Dissertation]. University of Arizona; 1972. Available from: http://hdl.handle.net/10150/287998

University of Texas Southwestern Medical Center

26. Roybal, Kole Thomas 1982-. Actin Regulatory Dynamics Required for T Cell Activation: A Quantitative and Systems-Level Perspective.

Degree: 2013, University of Texas Southwestern Medical Center

URL: http://hdl.handle.net/2152.5/ETD-UTSWMED-2013-05-91

► T cell activation occurs through interaction with an antigen-presenting cell (APC). Upon activation, signaling ensues with the coordination of dozens of diverse signaling molecules in… (more)

▼ T cell activation occurs through interaction with an antigen-presenting cell (APC). Upon activation, signaling ensues with the coordination of dozens of diverse signaling molecules in space and time, a feature of cell signaling we call ‘spatiotemporal patterning’. We performed a systems-scale analysis of the spatiotemporal patterning of T cell signaling and have found that it is highly diverse. Over 50 signaling sensors were imaged in live primary T cells activated with APCs under various physiological stimulation conditions, and no two signaling intermediates showed the same dynamic localization. The activation environment controlled spatiotemporal features of T cell signaling and specific spatiotemporal features correlated with efficient T cell activation. To identify underlying cell biological mechanisms controlling spatiotemporal organization of signaling, we complimented our live cell imaging with microscopy across multiple scales and identified a dense transient F-actin network that extends from a highly interdigitated T cell:APC interface several micrometers deep into the T cell lamellum. Systems-scale imaging revealed a large network of proximal T cell signaling intermediates that localized to the lamellal actin network and shared the spatial, temporal, and mobility features of F-actin. Interference with lamellal actin dynamics modulated the activity of the associated proteins and impaired IL-2 production. These data strongly suggest that the transient deep F-actin network by controlling lamellal localization modulates the activity of a substantial part of the T cell signal transduction system. As a next step in understanding how spatiotemporal dynamics of signaling controls T cell activation, we have developed a quantitative 4D analysis approach for signaling networks and coupled it with traditional cell biological techniques to uncover higher order mechanisms of the control of actin dynamics by CD28 co-stimulation during T cell activation. A group of nine actin regulatory proteins that mediate actin polymerization, capping, and severing were assessed and CD28 co-stimulation was required for their sustained activity at the T cell:APC interface. WAVE2 and Cofilin were especially sensitive to blockade of CD28 signaling. Functional relevance of the loss of WAVE2 and Cofilin enrichment was shown by the treatment of T cells with constitutively active Rac1 and Cofilin, which bypassed the requirement of co-stimulation for normal actin dynamics and AKT activation. This study highlights how a systems analysis of actin regulation could identify mechanisms that are inaccessible to more traditional single protein/gene approaches. Advisors/Committee Members: Ward, E. Sally, Wülfing, Christoph, Rosen, Michael K., Pasare, Chandrashekhar.

Subjects/Keywords: Actins; Signal Transduction; T-Lymphocytes

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APA (6^th Edition):

Roybal, K. T. 1. (2013). Actin Regulatory Dynamics Required for T Cell Activation: A Quantitative and Systems-Level Perspective. (Thesis). University of Texas Southwestern Medical Center. Retrieved from http://hdl.handle.net/2152.5/ETD-UTSWMED-2013-05-91

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Roybal, Kole Thomas 1982-. “Actin Regulatory Dynamics Required for T Cell Activation: A Quantitative and Systems-Level Perspective.” 2013. Thesis, University of Texas Southwestern Medical Center. Accessed June 24, 2019. http://hdl.handle.net/2152.5/ETD-UTSWMED-2013-05-91.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Roybal, Kole Thomas 1982-. “Actin Regulatory Dynamics Required for T Cell Activation: A Quantitative and Systems-Level Perspective.” 2013. Web. 24 Jun 2019.

Vancouver:

Roybal KT1. Actin Regulatory Dynamics Required for T Cell Activation: A Quantitative and Systems-Level Perspective. [Internet] [Thesis]. University of Texas Southwestern Medical Center; 2013. [cited 2019 Jun 24]. Available from: http://hdl.handle.net/2152.5/ETD-UTSWMED-2013-05-91.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Roybal KT1. Actin Regulatory Dynamics Required for T Cell Activation: A Quantitative and Systems-Level Perspective. [Thesis]. University of Texas Southwestern Medical Center; 2013. Available from: http://hdl.handle.net/2152.5/ETD-UTSWMED-2013-05-91

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Texas Southwestern Medical Center

27. Paek, So Yeon. Soluble Peptide Treatment Reverses CD8 T Cell-Induced Disease in a Mouse Model of Spontaneous Tissue-Selective Autoimmunity.

Degree: 2011, University of Texas Southwestern Medical Center

URL: http://hdl.handle.net/2152.5/927

► Autoimmunity is a complex process that involves recognition of self antigens by autoreactive T cells or tissue targeting by autoantibodies produced by B cells. Specific… (more)

Subjects/Keywords: Peptide Fragments; T-Lymphocytes; Autoimmunity

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APA (6^th Edition):

Paek, S. Y. (2011). Soluble Peptide Treatment Reverses CD8 T Cell-Induced Disease in a Mouse Model of Spontaneous Tissue-Selective Autoimmunity. (Thesis). University of Texas Southwestern Medical Center. Retrieved from http://hdl.handle.net/2152.5/927

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Paek, So Yeon. “Soluble Peptide Treatment Reverses CD8 T Cell-Induced Disease in a Mouse Model of Spontaneous Tissue-Selective Autoimmunity.” 2011. Thesis, University of Texas Southwestern Medical Center. Accessed June 24, 2019. http://hdl.handle.net/2152.5/927.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Paek, So Yeon. “Soluble Peptide Treatment Reverses CD8 T Cell-Induced Disease in a Mouse Model of Spontaneous Tissue-Selective Autoimmunity.” 2011. Web. 24 Jun 2019.

Vancouver:

Paek SY. Soluble Peptide Treatment Reverses CD8 T Cell-Induced Disease in a Mouse Model of Spontaneous Tissue-Selective Autoimmunity. [Internet] [Thesis]. University of Texas Southwestern Medical Center; 2011. [cited 2019 Jun 24]. Available from: http://hdl.handle.net/2152.5/927.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Paek SY. Soluble Peptide Treatment Reverses CD8 T Cell-Induced Disease in a Mouse Model of Spontaneous Tissue-Selective Autoimmunity. [Thesis]. University of Texas Southwestern Medical Center; 2011. Available from: http://hdl.handle.net/2152.5/927

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Texas Southwestern Medical Center

28. Harp, Christopher Todd. B Cell Modulation of T Cell Responses in Multiple Sclerosis.

Degree: 2010, University of Texas Southwestern Medical Center

URL: http://hdl.handle.net/2152.5/900

► Until recently, a definitive role for B cells in the pathogenesis of the autoimmune neurological disorder multiple sclerosis (MS), had not been widely accepted, and… (more)

▼ Until recently, a definitive role for B cells in the pathogenesis of the autoimmune neurological disorder multiple sclerosis (MS), had not been widely accepted, and remains poorly understood. B cells have multiple functions in the immune system and can both positively and negatively modulate immune responses through the production of antibody, cytokine secretion, and/or antigen presentation. Several studies indirectly suggest that B cell-T cell cooperation may be paramount in MS disease pathogenesis, although this interaction has not been well studied in MS. Therefore, the focus of my thesis project was to test the hypothesis that B cells could be efficient neuro-antigen presenting cells in the context of MS. My work has demonstrated that the cerebrospinal fluid (CSF) B cell population in MS shows characteristics of both auto-reactivity and antigen driven selection in a germinal center reaction. These findings suggest that neuro-antigen driven selection had occurred in the periphery and prompted investigation of B cells as neuro-antigen presenting cells. Examination of CD40 ligand (CD40L) and interleukin-4 (IL-4) activated peripheral B cells demonstrated for the first time that B cells could efficiently elicit myelin basic protein (MBP) specific CD8+ and CD4+ T cell proliferation from resting T cells in vitro through a mechanism that was partially dependent on presentation through HLA-DR. Further inquiry into the antigen presentation capacity of specific subpopulations of resting B cells revealed that memory B cells from MS patients (but not healthy donors (HDs)) were significantly better neuro-antigen specific presenting cells than their na?ve counter parts. This data indicated that a specific peripheral immune response had been generated in response to neuro-antigens in RRMS patients but not HDs. Taken together, these data provide a model where antigen experienced peripheral B cells from MS patients (but not HDs) provide important T cell support through antigen presentation and add to our understanding of the role of B cells in the pathogenesis of this autoimmune disease of the CNS. Advisors/Committee Members: Monson, Nancy L..

Subjects/Keywords: Multiple Sclerosis; B-Lymphocytes; Antigens

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Harp, C. T. (2010). B Cell Modulation of T Cell Responses in Multiple Sclerosis. (Thesis). University of Texas Southwestern Medical Center. Retrieved from http://hdl.handle.net/2152.5/900

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Harp, Christopher Todd. “B Cell Modulation of T Cell Responses in Multiple Sclerosis.” 2010. Thesis, University of Texas Southwestern Medical Center. Accessed June 24, 2019. http://hdl.handle.net/2152.5/900.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Harp, Christopher Todd. “B Cell Modulation of T Cell Responses in Multiple Sclerosis.” 2010. Web. 24 Jun 2019.

Vancouver:

Harp CT. B Cell Modulation of T Cell Responses in Multiple Sclerosis. [Internet] [Thesis]. University of Texas Southwestern Medical Center; 2010. [cited 2019 Jun 24]. Available from: http://hdl.handle.net/2152.5/900.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Harp CT. B Cell Modulation of T Cell Responses in Multiple Sclerosis. [Thesis]. University of Texas Southwestern Medical Center; 2010. Available from: http://hdl.handle.net/2152.5/900

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

29. Tohmé, Mira. Rôle des protéines chaperonnes, UNC93B1 et la chaine invariante, dans la signalisation des TLR7 et 9 dans les cellules B : Role of the chaperone molecules, UNC93B1 and invariant chain, in TLR7 and 9 signaling in B cells.

Degree: Docteur es, Immunologie, 2013, Université Paris Descartes – Paris V

URL: http://www.theses.fr/2013PA05T076

► La connaissance du système immunitaire est une étape clé dans la compréhension et la prévention des infections bactériennes, virales, des maladies auto-immunes et du cancer.… (more)

▼ La connaissance du système immunitaire est une étape clé dans la compréhension et la prévention des infections bactériennes, virales, des maladies auto-immunes et du cancer. Les lymphocytes B (LB) sont spécialisés dans la fonction de présentation de l'antigène et sont responsables de la production d'anticorps de haute affinité qui permettent l'élimination des agents infectieux. Après l'engagement du récepteur des cellules B (BCR), les LB polarisent rapidement leur centre organisateur des microtubules (MTOC) et les molécules du CMH de classe II vers l'interface BCR-Antigen (Ag), ce qui déclenche la libération de protéases lysosomales permettant l'extraction de l’Ag immobilisé à la surface des macrophages ou cellules dendritiques, un processus essentiel pour les LB à acquérir leur fonction de présentation antigénique. Toutefois, la signalisation par le BCR n'est pas le seul facteur déterminant dans l'activation des cellules B périphériques. La reconnaissance des motifs moléculaires de virus et bactéries par les Toll Like Receptors (TLRs) est importante dans la perte de tolérance des cellules B. Les TLR sont divisés en deux familles: les TLR1, 2, 4, 5, 6 et 11, qui détectent la présence de protéines et lipides bactériens et sont exprimés à la membrane plasmique, et les TLR3, 7-8 et 9, localisés dans les endosomes et qui détectent la présence d’ARN simple brin, ou double brin et ADN non méthylé respectivement. Compte tenu de leur localisation endosomale, les TLR3, 7, 8 et 9 sont des acteurs clés dans la discrimination entre le soi non-infectieux et le non-soi infectieux. Dans les cellules dendritiques, les TLR7 et 9 sont retenus dans le réticulum endoplasmique avec une molécule chaperonne, UNC93B1. Suite à leur stimulation, ils migrent vers les compartiments lysosomaux où ils subissent un clivage protéolytique par l'AEP, une asparagine endopeptidase, pour être fonctionnels. D'autre part, il a été récemment montré que les molécules MHC de classe II intracellulaires sont capables de promouvoir l’activation complète des TLR3, 4 et 9 via une interaction directe avec la tyrosine kinase Btk dans les macrophages et les cellules dendritiques. Notre travail a porté sur l’identification de nouvelles molécules régulant la signalisation des TLR7 et 9 dans les cellules B. Nos résultats, jusqu'à présent, montrent que la signalisation du TLR7 mais pas celle du TLR9 semble être régulée par la molécule chaperone associée au CMHII, la chaîne invariante ou Ii. En effet, dans les cellules B, au repos et après stimulation, le TLR7 réside dans les lysosomes avec la chaîne invariante. Après stimulation, la chaîne invariante interagit spécifiquement avec TLR7 et sa molécule adaptatrice MyD88 favorisant la sécrétion des cytokines pro-inflammatoires telles que L’IL-6 et le TNF-α. Étonnamment, en absence de Ii ou lorsque l'expression de Ii est diminuée suite à une infection virale, TLR7, mais pas TLR9, relocalise dans le réticulum endoplasmique (RE) conduisant ainsi à une exacerbation des fonctions innées du TLR7 telles que la production des… Advisors/Committee Members: Manoury, Bénédicte (thesis director).

Subjects/Keywords: Lymphocytes B; TLR7; Chaine invariante; B lymphocytes; TLR7; Invariant chain; 616.079

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Tohmé, M. (2013). Rôle des protéines chaperonnes, UNC93B1 et la chaine invariante, dans la signalisation des TLR7 et 9 dans les cellules B : Role of the chaperone molecules, UNC93B1 and invariant chain, in TLR7 and 9 signaling in B cells. (Doctoral Dissertation). Université Paris Descartes – Paris V. Retrieved from http://www.theses.fr/2013PA05T076

Chicago Manual of Style (16^th Edition):

Tohmé, Mira. “Rôle des protéines chaperonnes, UNC93B1 et la chaine invariante, dans la signalisation des TLR7 et 9 dans les cellules B : Role of the chaperone molecules, UNC93B1 and invariant chain, in TLR7 and 9 signaling in B cells.” 2013. Doctoral Dissertation, Université Paris Descartes – Paris V. Accessed June 24, 2019. http://www.theses.fr/2013PA05T076.

MLA Handbook (7^th Edition):

Tohmé, Mira. “Rôle des protéines chaperonnes, UNC93B1 et la chaine invariante, dans la signalisation des TLR7 et 9 dans les cellules B : Role of the chaperone molecules, UNC93B1 and invariant chain, in TLR7 and 9 signaling in B cells.” 2013. Web. 24 Jun 2019.

Vancouver:

Tohmé M. Rôle des protéines chaperonnes, UNC93B1 et la chaine invariante, dans la signalisation des TLR7 et 9 dans les cellules B : Role of the chaperone molecules, UNC93B1 and invariant chain, in TLR7 and 9 signaling in B cells. [Internet] [Doctoral dissertation]. Université Paris Descartes – Paris V; 2013. [cited 2019 Jun 24]. Available from: http://www.theses.fr/2013PA05T076.

Council of Science Editors:

Tohmé M. Rôle des protéines chaperonnes, UNC93B1 et la chaine invariante, dans la signalisation des TLR7 et 9 dans les cellules B : Role of the chaperone molecules, UNC93B1 and invariant chain, in TLR7 and 9 signaling in B cells. [Doctoral Dissertation]. Université Paris Descartes – Paris V; 2013. Available from: http://www.theses.fr/2013PA05T076

30. Benbarche, Salima. Influence des cytokines immunitaires sur la mégacaryocytopoïèse et l'homéostasie des plaquettes sanguines : rôle de l'interleukine 21 : Effect of immune cytokines on megakaryocytopoiesis and platelet homeostasis : role of interleukin 21.

Degree: Docteur es, Sciences de la vie et de la santé, 2015, Université de Strasbourg

URL: http://www.theses.fr/2015STRAJ044

►

La mégacaryocytopoïèse est le processus de différenciation des cellules souches hématopoïétiques en mégacaryocytes produisant les plaquettes sanguines. Des données de transcriptome indiquent la présence des… (more)

▼

La mégacaryocytopoïèse est le processus de différenciation des cellules souches hématopoïétiques en mégacaryocytes produisant les plaquettes sanguines. Des données de transcriptome indiquent la présence des récepteurs aux interleukines (IL) -10, -17A et -21 sur les mégacaryocytes. Mon travail montre que les IL-10 et -17 n’ont pas d’effet apparent sur la prolifération et la différenciation in vitro des mégacaryocytes à partir des progéniteurs hématopoïétiques humains CD34+. Cependant, l’IL-21 augmente la prolifération des progéniteurs mégacaryocytaires dérivés de ces cellules et sans modifier leur différenciation. Les mégacaryocytes de la moelle osseuse humaine expriment aussi le récepteur à l’IL-21, ce qui suggère un rôle direct de l’IL-21 sur la mégacaryocytopoïèse in vivo. De façon concordante, l’expression de l’IL-21 chez la souris stimule la mégacaryocytopoïèse ainsi que la production des plaquettes, mais augmente la clairance des plaquettes par les macrophages. Ces travaux suggèrent que durant les réponses immunitaires, l’expression de l’IL-21 par les lymphocytes T CD4+ activés module l’homéostasie des plaquettes sanguines.

Megakaryocytopoiesis is the process by which hematopoietic stem cells give rise to megakaryocytes which in turn produce blood platelets. Transcriptional studies indicate the presence of interleukin (IL) -10, -17 and -21 receptors on megakaryocytes. This work shows that IL-10 and -17 have no apparent effect on in vitro proliferation and differentiation of megakaryocytes derived from human CD34+ hematopoietic progenitors. However, IL-21 increases the proliferation of megakaryocyte progenitors derived from these CD34+ cells without modifying their differentiation. Moreover, human bone marrow megakaryocytes express IL-21 receptor, suggesting a direct role of IL-21 on megakaryocytopoiesis in vivo. Concordantly, IL-21 expression in mice stimulates megakaryocytopoiesis and platelet production, but increases platelet clearance by macrophages. This work suggest that during immune responses, the expression of IL-21 by activated CD4+ T lymphocytes modulate blood platelet homeostasis.

Advisors/Committee Members: De la Salle, Henri (thesis director).

Subjects/Keywords: Mégacaryocytopoïèse; Plaquettes; IL-21; Lymphocytes; Megakaryocytopoiesis; Platelets; IL-21; Lymphocytes; 573.1

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Benbarche, S. (2015). Influence des cytokines immunitaires sur la mégacaryocytopoïèse et l'homéostasie des plaquettes sanguines : rôle de l'interleukine 21 : Effect of immune cytokines on megakaryocytopoiesis and platelet homeostasis : role of interleukin 21. (Doctoral Dissertation). Université de Strasbourg. Retrieved from http://www.theses.fr/2015STRAJ044

Chicago Manual of Style (16^th Edition):

Benbarche, Salima. “Influence des cytokines immunitaires sur la mégacaryocytopoïèse et l'homéostasie des plaquettes sanguines : rôle de l'interleukine 21 : Effect of immune cytokines on megakaryocytopoiesis and platelet homeostasis : role of interleukin 21.” 2015. Doctoral Dissertation, Université de Strasbourg. Accessed June 24, 2019. http://www.theses.fr/2015STRAJ044.

MLA Handbook (7^th Edition):

Benbarche, Salima. “Influence des cytokines immunitaires sur la mégacaryocytopoïèse et l'homéostasie des plaquettes sanguines : rôle de l'interleukine 21 : Effect of immune cytokines on megakaryocytopoiesis and platelet homeostasis : role of interleukin 21.” 2015. Web. 24 Jun 2019.

Vancouver:

Benbarche S. Influence des cytokines immunitaires sur la mégacaryocytopoïèse et l'homéostasie des plaquettes sanguines : rôle de l'interleukine 21 : Effect of immune cytokines on megakaryocytopoiesis and platelet homeostasis : role of interleukin 21. [Internet] [Doctoral dissertation]. Université de Strasbourg; 2015. [cited 2019 Jun 24]. Available from: http://www.theses.fr/2015STRAJ044.

Council of Science Editors:

Benbarche S. Influence des cytokines immunitaires sur la mégacaryocytopoïèse et l'homéostasie des plaquettes sanguines : rôle de l'interleukine 21 : Effect of immune cytokines on megakaryocytopoiesis and platelet homeostasis : role of interleukin 21. [Doctoral Dissertation]. Université de Strasbourg; 2015. Available from: http://www.theses.fr/2015STRAJ044

Open Access Theses and Dissertations