subject:(liposomes)

Oregon State University

1. Parker, Robert S. The use of liposomes as encapsulating agents for feeding juvenile Pacific oysters (Crassostrea gigas).

Degree: PhD, Food Science and Technology, 1980, Oregon State University

URL: http://hdl.handle.net/1957/27395

► The ingestion, uptake, and metabolism of liposomes by juvenile Pacific oysters (Crassostrea gigas) were studied by several methods in an effort to assess their potential… (more)

▼ The ingestion, uptake, and metabolism of liposomes by juvenile Pacific oysters (Crassostrea gigas) were studied by several methods in an effort to assess their potential as encapsulating agents. Liposomes composed of egg phosphatidylcholine-cholesterol-stearylamine (7:1:2) formed readily and appeared stable in 20°/oo seawater. Radiotracer studies with liposomes made with ¹⁴C-labeled cholesterol or phosphatidylcholine showed uptake of up to 40% of the dose in 24 hrs, with the majority of uptake occurring in the visceral mass. Only slight amounts of label were observed in adductor muscle or mantle tissue. Absence of label in free fatty acids in oysters fed liposomes made with di[l-¹⁴C] palmitoyl phosphatidylcholine indicated a lack of significant amounts of fatty acid hydrolysis from phospholipid in the stomach or lumen of the digestive diverticula. However, radioactivity was observed in lipid other than phosphatidylcholine, including triglyceride, phosphatidylethanolamine, and an unidentified polar lipid. Radioactivity in these lipids resided exclusively in the fatty acids, indicating breakdown of the ¹⁴C-phosphatidylcholine via acyl transfer. To examine metabolism of liposome-encapsulated substances, [1-¹⁴C]glucose and [U-¹⁴C]amino acids were entrapped and fed to oysters. Label from glucose appeared largely in a choloroform-methanol-insoluble fraction, with little radioactivity recovered in the lipid or soluble aqueous fractions. Most label from amino acids was recovered in trichloroacetic acid-precipitable protein. Control oysters given the same amounts of non-encapsulated [1-¹⁴C] glucose or [U-¹⁴C]amino acids as in liposome trials showed (1) the same uptake of label from free amino acids in comparison with encapsulated glucose, and (2) increased uptake of free, amino acids in comparison with encapsulated amino acids. Label from free glucose or amino acids entered the same fractions as encapsulated label. Evidence for intracellular uptake of liposomes was obtained with fluorescence microscopy after feeding oysters with liposomes containing bovine serum albumin conjugated with fluorescein isothiocyante (FITC). The appearance of small fluorescent inclusions within the apical portions of many of the ducts and tubules of the digestive diverticula suggest phagocytosis of intact liposomes. Uptake was not observed in other parts of the alimentary canal. The feeding of liposomes in which the stearylamine had been conjugated with FITC resulted in generalized fluorescence in most of the digestive diverticula and stomach epithelium, perhaps due to extracellular hydrolysis of FITC and its subsequent diffusion into epithelial cells. No fluorescence occurred in tissues other than those of the digestive tract. Autoradiography studies with liposomes containing di[l-¹⁴C]palmitoyl phosphatidylcholine showed radioactivity dispersed throughout the epithelial cells of the ducts and tubules of the digestive diverticula. Only slight radioactivity was observed in the intertubular connective… Advisors/Committee Members: Selivonchick, D. P. (advisor).

Subjects/Keywords: Liposomes

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APA (6^th Edition):

Parker, R. S. (1980). The use of liposomes as encapsulating agents for feeding juvenile Pacific oysters (Crassostrea gigas). (Doctoral Dissertation). Oregon State University. Retrieved from http://hdl.handle.net/1957/27395

Chicago Manual of Style (16^th Edition):

Parker, Robert S. “The use of liposomes as encapsulating agents for feeding juvenile Pacific oysters (Crassostrea gigas).” 1980. Doctoral Dissertation, Oregon State University. Accessed March 07, 2021. http://hdl.handle.net/1957/27395.

MLA Handbook (7^th Edition):

Parker, Robert S. “The use of liposomes as encapsulating agents for feeding juvenile Pacific oysters (Crassostrea gigas).” 1980. Web. 07 Mar 2021.

Vancouver:

Parker RS. The use of liposomes as encapsulating agents for feeding juvenile Pacific oysters (Crassostrea gigas). [Internet] [Doctoral dissertation]. Oregon State University; 1980. [cited 2021 Mar 07]. Available from: http://hdl.handle.net/1957/27395.

Council of Science Editors:

Parker RS. The use of liposomes as encapsulating agents for feeding juvenile Pacific oysters (Crassostrea gigas). [Doctoral Dissertation]. Oregon State University; 1980. Available from: http://hdl.handle.net/1957/27395

2. Pachis, Konstantinos. Ανάπτυξη λιποσωμικών μορφών μη-στεροειδών αντιφλεγμονωδών φαρμάκων (ΜΣΑΦ) για οφθαλμική χορήγηση.

Degree: 2017, University of Patras; Πανεπιστήμιο Πατρών

URL: http://hdl.handle.net/10442/hedi/41732

► Non-steroidal anti-inflammatory drugs (NSAIDs) are cyclooxygenase (an enzyme that is involved in the metabolism of arachidonic acid into prostaglandins) inhibitors, and therefore, they inhibit the… (more)

▼ Non-steroidal anti-inflammatory drugs (NSAIDs) are cyclooxygenase (an enzyme that is involved in the metabolism of arachidonic acid into prostaglandins) inhibitors, and therefore, they inhibit the onset of inflammation. In ophthalmology, they are mainly used as eyedrops for topical administration, for therapy of inflammation, such as post-operative macular edema, without always providing the desired efficacy. Their intravitreal use has been tested in recent years for the cystic edema related with various types of macular degeneration, such as postoperative, diabetic, uveitis, with interesting results. Intravitreal (IVT) administration is known to provide significantly higher drug levels in the retina (compared to topical or systemic administration), while minimizing systemic adverse effects. The main drawback of these drugs is their short half-life in the vitreous cavity, which requires frequent injections to achieve the therapeutic effect. In order to develop a system for prolonged release of NSAIDs from the posterior segment of the eye, an in-situ liposome aggregation system (liposomal platform), was developed. For initial proof of the concept the fluorescent molecules of calcein and FITC-Dextran were used as model-drugs, after being encapsulate in liposomes. The system was studied in vitro and in vivo studies in order to confirm its functionality of. Subsequently, lornoxicam and flurbiprofen (FLB) liposomes were then prepared using a variety of liposome preparation techniques and optimized with the aim to achieve highest encapsulation efficiency and lowest particle size, in order to select the best drug to continue studies with. Actively loaded FLB Liposomes (with lipid composition of HPC/DPPG/Chol (9/1/10 mol: mol)), had the highest encapsulation yield and were thus selected. In vitro physicochemical characterization and drug release kinetics were subsequently evaluated. FLB solution, and plain liposomes were used as reference formulations.A second sustained release system was also developed; a thermosensitive hydrogel based on Pluronic F127 polymer, which incorporated FLB or FLB liposomes. In vitro release studies were conducted in order to investigate the optimal polymer concentration that would yield the slowest release rate. FL solution, plain liposomes and simple hydrogel were used as reference formulations. The optimal Pluronic F127 concentration was 18% w/v.In vitro studies of both hybrid liposomal systems, the liposomal platform and the liposomal hydrogel, confirmed that they should provide significant prolongation of the retention of the drug in the eye, in relation to the reference formulations. The liposomal platform released 20% of entrapped drug after 24 hours, while the liposomal hydrogel released 30% at the same time period. Both systems released measurable amounts of FLB for up to 120 h. Conclusively, two novel hybridic liposomal systems have that succeed to significantly extend the residence time of flurbiprofen (in vitro) and are suitable for intraocular administration, have been…

Subjects/Keywords: Λιποσώματα; Liposomes

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APA (6^th Edition):

Pachis, K. (2017). Ανάπτυξη λιποσωμικών μορφών μη-στεροειδών αντιφλεγμονωδών φαρμάκων (ΜΣΑΦ) για οφθαλμική χορήγηση. (Thesis). University of Patras; Πανεπιστήμιο Πατρών. Retrieved from http://hdl.handle.net/10442/hedi/41732

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Pachis, Konstantinos. “Ανάπτυξη λιποσωμικών μορφών μη-στεροειδών αντιφλεγμονωδών φαρμάκων (ΜΣΑΦ) για οφθαλμική χορήγηση.” 2017. Thesis, University of Patras; Πανεπιστήμιο Πατρών. Accessed March 07, 2021. http://hdl.handle.net/10442/hedi/41732.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Pachis, Konstantinos. “Ανάπτυξη λιποσωμικών μορφών μη-στεροειδών αντιφλεγμονωδών φαρμάκων (ΜΣΑΦ) για οφθαλμική χορήγηση.” 2017. Web. 07 Mar 2021.

Vancouver:

Pachis K. Ανάπτυξη λιποσωμικών μορφών μη-στεροειδών αντιφλεγμονωδών φαρμάκων (ΜΣΑΦ) για οφθαλμική χορήγηση. [Internet] [Thesis]. University of Patras; Πανεπιστήμιο Πατρών; 2017. [cited 2021 Mar 07]. Available from: http://hdl.handle.net/10442/hedi/41732.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Pachis K. Ανάπτυξη λιποσωμικών μορφών μη-στεροειδών αντιφλεγμονωδών φαρμάκων (ΜΣΑΦ) για οφθαλμική χορήγηση. [Thesis]. University of Patras; Πανεπιστήμιο Πατρών; 2017. Available from: http://hdl.handle.net/10442/hedi/41732

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Oregon State University

3. Sorasuchart, Waranush. Evaluation of polycarbophil coated liposomes and membrane permeation of free and liposomal drugs: In vitro-in vivo evaluation of nicardipine HCl sustained-release formulations.

Degree: PhD, Pharmacy, 1998, Oregon State University

URL: http://hdl.handle.net/1957/33656

Subjects/Keywords: Liposomes

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Sorasuchart, W. (1998). Evaluation of polycarbophil coated liposomes and membrane permeation of free and liposomal drugs: In vitro-in vivo evaluation of nicardipine HCl sustained-release formulations. (Doctoral Dissertation). Oregon State University. Retrieved from http://hdl.handle.net/1957/33656

Chicago Manual of Style (16^th Edition):

Sorasuchart, Waranush. “Evaluation of polycarbophil coated liposomes and membrane permeation of free and liposomal drugs: In vitro-in vivo evaluation of nicardipine HCl sustained-release formulations.” 1998. Doctoral Dissertation, Oregon State University. Accessed March 07, 2021. http://hdl.handle.net/1957/33656.

MLA Handbook (7^th Edition):

Sorasuchart, Waranush. “Evaluation of polycarbophil coated liposomes and membrane permeation of free and liposomal drugs: In vitro-in vivo evaluation of nicardipine HCl sustained-release formulations.” 1998. Web. 07 Mar 2021.

Vancouver:

Sorasuchart W. Evaluation of polycarbophil coated liposomes and membrane permeation of free and liposomal drugs: In vitro-in vivo evaluation of nicardipine HCl sustained-release formulations. [Internet] [Doctoral dissertation]. Oregon State University; 1998. [cited 2021 Mar 07]. Available from: http://hdl.handle.net/1957/33656.

Council of Science Editors:

Sorasuchart W. Evaluation of polycarbophil coated liposomes and membrane permeation of free and liposomal drugs: In vitro-in vivo evaluation of nicardipine HCl sustained-release formulations. [Doctoral Dissertation]. Oregon State University; 1998. Available from: http://hdl.handle.net/1957/33656

University of Illinois – Chicago

4. Iwasaki, Eri. Preparation and In Vitro Analysis of Dendrimer-encapsulated Liposomes.

Degree: 2013, University of Illinois – Chicago

URL: http://hdl.handle.net/10027/10130

► Since most of currently available cancer chemotherapeutics often come with severe side effects caused by high toxicity to healthy cells and tissues, it is important… (more)

▼ Since most of currently available cancer chemotherapeutics often come with severe side effects caused by high toxicity to healthy cells and tissues, it is important to design a novel platform to deliver drugs specifically to the target tissue. In order to minimize these side effects, a number of nanoparticle-based therapeutic, including liposomes, polymeric nanoparticles, micelles, dendrimers, and metallic nanoparticles, has been developed in the last few decades. Consequently, application of these nanotechnologies in the medical field has enhanced the therapeutic activity and reduced the toxic side effects of cancer drugs. In this study, G2 PAMAM dendrimer conjugates (G2-RITC-NH2) were encapsulated into liposomes consisting of DMPC, DSPE-PEG-2000, and cholesterol using a lipid film hydration method. Three different liposomal formulations were prepared by varying the cholesterol content at 25, 50, and 75 mol%. The liposomes were then investigated in terms of (i) release kinetics of G2-RITC-NH2 from each liposome, (ii) cellular uptake kinetics, (iii) endocytosis mechanisms, and (iv) cytotoxicity. The release profiles of G2-RITC-NH2 from each liposome revealed that the stability of the liposomes is highly dependent on the cholesterol level in the lipid bilayer. Microscope observations and flow cytometry measurements also showed the different internalization kinetics of the three liposomal formulations from that of unencapsulated G2-RITC-NH2. In order to further investigate the endocytosis mechanisms of these materials, MCF-7 cells were pre-treated with 5 mM MβCD before being incubated with unencapsulated G2-RITC-NH2 and the liposomal formulations up to 24 h. The results obtained from the flow cytometry analysis indicated that G2-RITC-NH2 internalizes into the cells by a different mechanism compared to the liposomes. Finally, cytotoxicity of G2-RITC-NH2 and the three liposomal formulations was investigated, which showed no significant inhibition of cell proliferation up to 10 µM normalized based on the G2-RITC-NH2 concentration, indicating that the materials could be used as a drug delivery platform. In summary, the in vitro characterization experiments conducted in this study serve as a critical starting point to engineer effective drug delivery systems that combine the advantages of dendrimers and liposomes. Advisors/Committee Members: Hong, Seungpyo (advisor), Eddington, David (committee member), Gemeinhart, Richard (committee member).

Subjects/Keywords: Dendrimers; Liposomes

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APA (6^th Edition):

Iwasaki, E. (2013). Preparation and In Vitro Analysis of Dendrimer-encapsulated Liposomes. (Thesis). University of Illinois – Chicago. Retrieved from http://hdl.handle.net/10027/10130

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Iwasaki, Eri. “Preparation and In Vitro Analysis of Dendrimer-encapsulated Liposomes.” 2013. Thesis, University of Illinois – Chicago. Accessed March 07, 2021. http://hdl.handle.net/10027/10130.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Iwasaki, Eri. “Preparation and In Vitro Analysis of Dendrimer-encapsulated Liposomes.” 2013. Web. 07 Mar 2021.

Vancouver:

Iwasaki E. Preparation and In Vitro Analysis of Dendrimer-encapsulated Liposomes. [Internet] [Thesis]. University of Illinois – Chicago; 2013. [cited 2021 Mar 07]. Available from: http://hdl.handle.net/10027/10130.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Iwasaki E. Preparation and In Vitro Analysis of Dendrimer-encapsulated Liposomes. [Thesis]. University of Illinois – Chicago; 2013. Available from: http://hdl.handle.net/10027/10130

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Simon Fraser University

5. Hunter, David Gregory. The effects of lipid composition and extrusion pressure and temperature on the properties of phospholipid vesicles.

Degree: 1997, Simon Fraser University

URL: http://summit.sfu.ca/item/7454

Subjects/Keywords: Liposomes.

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APA (6^th Edition):

Hunter, D. G. (1997). The effects of lipid composition and extrusion pressure and temperature on the properties of phospholipid vesicles. (Thesis). Simon Fraser University. Retrieved from http://summit.sfu.ca/item/7454

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Hunter, David Gregory. “The effects of lipid composition and extrusion pressure and temperature on the properties of phospholipid vesicles.” 1997. Thesis, Simon Fraser University. Accessed March 07, 2021. http://summit.sfu.ca/item/7454.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Hunter, David Gregory. “The effects of lipid composition and extrusion pressure and temperature on the properties of phospholipid vesicles.” 1997. Web. 07 Mar 2021.

Vancouver:

Hunter DG. The effects of lipid composition and extrusion pressure and temperature on the properties of phospholipid vesicles. [Internet] [Thesis]. Simon Fraser University; 1997. [cited 2021 Mar 07]. Available from: http://summit.sfu.ca/item/7454.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Hunter DG. The effects of lipid composition and extrusion pressure and temperature on the properties of phospholipid vesicles. [Thesis]. Simon Fraser University; 1997. Available from: http://summit.sfu.ca/item/7454

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Penn State University

6. Vanarsdale, Eric Scott. Fabrication and Characterization of Hydrogel-filled Nanoliposomes for Applications in Nanomedicine.

Degree: 2015, Penn State University

URL: https://submit-etda.libraries.psu.edu/catalog/27445

► Nano-scale carriers that enable selective delivery of bioactive payloads to specific cells are sought after in gene therapy, regenerative medicine, and cancer treatment. Nano-liposomes have… (more)

Subjects/Keywords: liposomes; nanomedicine; nanoparticles

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APA (6^th Edition):

Vanarsdale, E. S. (2015). Fabrication and Characterization of Hydrogel-filled Nanoliposomes for Applications in Nanomedicine. (Thesis). Penn State University. Retrieved from https://submit-etda.libraries.psu.edu/catalog/27445

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Vanarsdale, Eric Scott. “Fabrication and Characterization of Hydrogel-filled Nanoliposomes for Applications in Nanomedicine.” 2015. Thesis, Penn State University. Accessed March 07, 2021. https://submit-etda.libraries.psu.edu/catalog/27445.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Vanarsdale, Eric Scott. “Fabrication and Characterization of Hydrogel-filled Nanoliposomes for Applications in Nanomedicine.” 2015. Web. 07 Mar 2021.

Vancouver:

Vanarsdale ES. Fabrication and Characterization of Hydrogel-filled Nanoliposomes for Applications in Nanomedicine. [Internet] [Thesis]. Penn State University; 2015. [cited 2021 Mar 07]. Available from: https://submit-etda.libraries.psu.edu/catalog/27445.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Vanarsdale ES. Fabrication and Characterization of Hydrogel-filled Nanoliposomes for Applications in Nanomedicine. [Thesis]. Penn State University; 2015. Available from: https://submit-etda.libraries.psu.edu/catalog/27445

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

McMaster University

7. González Gómez, Azucena. DEVELOPING LIPOSOMES FOR ANTIBIOTIC ENCAPSULATION.

Degree: MASc, 2019, McMaster University

URL: http://hdl.handle.net/11375/23803

►

Liposomes are self-assembled lipid vesicles made from phospholipids that are safe and suitable for drug encapsulation and localized drug delivery. Liposomal formulations are characterized by… (more)

▼

Liposomes are self-assembled lipid vesicles made from phospholipids that are safe and suitable for drug encapsulation and localized drug delivery. Liposomal formulations are characterized by low toxicity and improved therapeutic index (by changing drug biodistribution) and liposomes encapsulating antifungal or anticancer drugs have already been approvedby regulatory agencies.One area of application for liposomes is localized antibiotic delivery. Antibiotics target bacteria, but specific types of infections(namely biofilms or intracellular infections)that required high or prolonged antibiotic administration have long been a challenge for antibiotic treatments. Liposomal delivery of antibiotics can improve their therapeutic index while minimizing their adverse effects. When it comes to methods of antibiotic encapsulation, however,most reports to date follow the methods developed for anticancer drugs for encapsulating antibiotics. This oversight causes discrepancies in the literature, mainly because of the significantly different chemical structures of antibiotics and cancer drugs. Furthermore, most antibiotics are highly sensitive to temperature fluctuations, which is concerning, given most liposomal preparation methods involve extreme temperature fluctuations. The aim of my thesis was to explore these missing links in the literature by answering these questions: (1) will liposome preparation method affect encapsulation efficiency of antibiotics?And (2) does liposomal preparation method adversely affect the efficacy of antibiotics?Investigating these questions led to further insight into the optimal process for achieving high encapsulation efficiencies for different antibiotics and for further avoiding damage due to harsh processing conditions. We found that different preparation methods are better for different types of antibiotics, being the one that promotes a large aqueous space better for hydrophilic drugs and the one that creates oligolamellar and large unilamellar vesicles better for more hydrophobic drugs. The steps in liposome preparation methods such as heating and sonication can affect the stability of the antibiotics.

Thesis

Master of Applied Science (MASc)

When antibiotics are administered, orally or intravenously, they should pass through different tissues to arrive to the site of infection; this can cause dilution and/or intoxication. To overcome these problems, drug delivery vehicles have been used to encapsulate and deliver antibiotics, improving their therapeutic index while minimizing their adverse effects. Liposomes are vesicles composed of at least one lipid bilayer, with an inner aqueous compartment. Liposomes are an attractive vehicle to deliver antibiotics because they can encapsulate both hydrophobic and hydrophilic antibiotics, they have low toxicity, and they can change the bio-distribution of the drug. In mythesis, I addressedtwo main questions regarding liposomal antibiotic encapsulation:(1) will liposome preparation method affect encapsulation efficiency of antibiotics, and(2)…

Advisors/Committee Members: Hosseini-Doust, Zeinab, Chemical Engineering.

Subjects/Keywords: Liposomes; antibiotics; nanoparticles

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APA (6^th Edition):

González Gómez, A. (2019). DEVELOPING LIPOSOMES FOR ANTIBIOTIC ENCAPSULATION. (Masters Thesis). McMaster University. Retrieved from http://hdl.handle.net/11375/23803

Chicago Manual of Style (16^th Edition):

González Gómez, Azucena. “DEVELOPING LIPOSOMES FOR ANTIBIOTIC ENCAPSULATION.” 2019. Masters Thesis, McMaster University. Accessed March 07, 2021. http://hdl.handle.net/11375/23803.

MLA Handbook (7^th Edition):

González Gómez, Azucena. “DEVELOPING LIPOSOMES FOR ANTIBIOTIC ENCAPSULATION.” 2019. Web. 07 Mar 2021.

Vancouver:

González Gómez A. DEVELOPING LIPOSOMES FOR ANTIBIOTIC ENCAPSULATION. [Internet] [Masters thesis]. McMaster University; 2019. [cited 2021 Mar 07]. Available from: http://hdl.handle.net/11375/23803.

Council of Science Editors:

González Gómez A. DEVELOPING LIPOSOMES FOR ANTIBIOTIC ENCAPSULATION. [Masters Thesis]. McMaster University; 2019. Available from: http://hdl.handle.net/11375/23803

8. Collins, James R. The remineralization of marine organic matter by diverse biological and abiotic processes.

Degree: 2017, MIT and Woods Hole Oceanographic Institution

URL: http://hdl.handle.net/1912/8721

► While aerobic respiration is typically invoked as the dominant mass-balance sink for organic matter in the upper ocean, many other biological and abiotic processes can… (more)

▼ While aerobic respiration is typically invoked as the dominant mass-balance sink for organic matter in the upper ocean, many other biological and abiotic processes can degrade particulate and dissolved substrates on globally significant scales. The relative strengths of these other remineralization processes — including mechanical mechanisms such as dissolution and disaggregation of sinking particles, and abiotic processes such as photooxidation — remain poorly constrained. In this thesis, I examine the biogeochemical significance of various alternative pathways of organic matter remineralization using a combination of field experiments, modeling approaches, geochemical analyses, and a new, high-throughput lipidomics method for identification of lipid biomarkers. I first assess the relative importance of particleattached microbial respiration compared to other processes that can degrade sinking marine particles. A hybrid methodological approach — comparison of substrate-specific respiration rates from across the North Atlantic basin with Monte Carlo-style sensitivity analyses of a simple mechanistic model — suggested sinking particle material was transferred to the water column by various biological and mechanical processes nearly 3.5 times as fast as it was directly respired, questioning the conventional assumption that direct respiration dominates remineralization. I next present and demonstrate a new lipidomics method and open-source software package for discovery and identification of molecular biomarkers for organic matter degradation in large, high-mass-accuracy HPLC-ESI-MS datasets. I use the software to unambiguously identify more than 1,100 unique lipids, oxidized lipids, and oxylipins in data from cultures of the marine diatom Phaeodactylum tricornutum that were subjected to oxidative stress. Finally, I present the results of photooxidation experiments conducted with liposomes — nonliving aggregations of lipids — in natural waters of the Southern Ocean. A broadband polychromatic apparent quantum yield (AQY) is applied to estimate rates of lipid photooxidation in surface waters of the West Antarctic Peninsula, which receive seasonally elevated doses of ultraviolet radiation as a consequence of anthropogenic ozone depletion in the stratosphere. The mean daily rate of lipid photooxidation (50 ± 11 pmol IP-DAG L−1 d−1, equivalent to 31 ± 7 𝜇g C m−3 d−1) represented between 2 and 8 % of the total bacterial production observed in surface waters immediately following the retreat of the sea ice.

Subjects/Keywords: Microorganisms; Respiration; Liposomes

11 more images…

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APA (6^th Edition):

Collins, J. R. (2017). The remineralization of marine organic matter by diverse biological and abiotic processes. (Thesis). MIT and Woods Hole Oceanographic Institution. Retrieved from http://hdl.handle.net/1912/8721

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Collins, James R. “The remineralization of marine organic matter by diverse biological and abiotic processes.” 2017. Thesis, MIT and Woods Hole Oceanographic Institution. Accessed March 07, 2021. http://hdl.handle.net/1912/8721.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Collins, James R. “The remineralization of marine organic matter by diverse biological and abiotic processes.” 2017. Web. 07 Mar 2021.

Vancouver:

Collins JR. The remineralization of marine organic matter by diverse biological and abiotic processes. [Internet] [Thesis]. MIT and Woods Hole Oceanographic Institution; 2017. [cited 2021 Mar 07]. Available from: http://hdl.handle.net/1912/8721.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Collins JR. The remineralization of marine organic matter by diverse biological and abiotic processes. [Thesis]. MIT and Woods Hole Oceanographic Institution; 2017. Available from: http://hdl.handle.net/1912/8721

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Alberta

9. Shahin, Mostafa H. Development of polymer and lipid based nano-delivery systems for targeted cancer chemotherapy.

Degree: PhD, Faculty of Pharmacy and Pharmaceutical Sciences, 2012, University of Alberta

URL: https://era.library.ualberta.ca/files/k0698856b

► Conventional chemotherapy agents can kill tumor cells and inhibit tumor growth, but they produce severe side effects on normal cells at the same time. To… (more)

▼ Conventional chemotherapy agents can kill tumor cells and inhibit tumor growth, but they produce severe side effects on normal cells at the same time. To shift the balance towards tumoral effects, it would be desirable to direct the anti-cancer drug towards tumor while restricting drug access to normal tissues. The main objective of this thesis was to develop tumor targeted drug delivery system that can take on this task and as a result, improve the specificity and anticancer activity of the incorporated anticancer drugs towards tumor. To this end, lipid and block copolymer based nano-delivery systems of two conventional anti-cancer agents doxorubicin (DOX) and paclitaxel (PTX) are developed, respectively, and modified on their surface with a 12mer breast tumor interacting peptide, namely p160, or its engineered derivatives developed in our research team. The effect of peptide decoration on the specific interaction as well as anti-cancer activity of developed nano-formulations against human breast tumor cells over normal epithelial breast cells or endothelial cells was characterized, in vitro. In this study, micelles of poly(ethylene oxide)-b-poly(-caprolactone) were prepared and modified with either c(RGDfK) or p160 and loaded with paclitaxel (PTX). Peptide decoration enhanced the selective cytotoxicity of encapsulated PTX against cancer cells over normal cells. The extent of this increase in cancer cell specificity for encapsulated PTX was more for p160-modified micelles. At the end, the anti-cancer activity of liposomal formulations of DOX, having different density of an engineered breast tumor targeting peptide, namely p-18-4, was assessed in vitro for cellular uptake and selective cytotoxicity, and in vivo using animal model of human breast tumor xenograft and compared to that for liposomal formulations of DOX with no peptide decoration. Liposomal DOX formulations bearing low p18-4 density showed better in vitro selective cytotoxicity and in vivo therapeutic efficacy. Our results points to the potential of p160 and its engineered derivatives as efficient ligands on lipid and block copolymer based nanocarriers for active targeting of anticancer agents to breast tumors. The results also show the success of this strategy in enhancing the specific anti-cancer effects of the incorporated drug against breast tumor models.

Subjects/Keywords: peptide; liposomes; chemotherapy; polymeric micelle

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Shahin, M. H. (2012). Development of polymer and lipid based nano-delivery systems for targeted cancer chemotherapy. (Doctoral Dissertation). University of Alberta. Retrieved from https://era.library.ualberta.ca/files/k0698856b

Chicago Manual of Style (16^th Edition):

Shahin, Mostafa H. “Development of polymer and lipid based nano-delivery systems for targeted cancer chemotherapy.” 2012. Doctoral Dissertation, University of Alberta. Accessed March 07, 2021. https://era.library.ualberta.ca/files/k0698856b.

MLA Handbook (7^th Edition):

Shahin, Mostafa H. “Development of polymer and lipid based nano-delivery systems for targeted cancer chemotherapy.” 2012. Web. 07 Mar 2021.

Vancouver:

Shahin MH. Development of polymer and lipid based nano-delivery systems for targeted cancer chemotherapy. [Internet] [Doctoral dissertation]. University of Alberta; 2012. [cited 2021 Mar 07]. Available from: https://era.library.ualberta.ca/files/k0698856b.

Council of Science Editors:

Shahin MH. Development of polymer and lipid based nano-delivery systems for targeted cancer chemotherapy. [Doctoral Dissertation]. University of Alberta; 2012. Available from: https://era.library.ualberta.ca/files/k0698856b

University of Alberta

10. Fitzsimmons, Ross. Novel polymer and lipid-based nanocarriers for gene delivery.

Degree: MS, Department of Biomedical Engineering, 2011, University of Alberta

URL: https://era.library.ualberta.ca/files/gf06g346v

► The following thesis describes original studies assessing the gene delivery efficacy of novel non-viral carrier combinations. The first panel of non-viral carriers tested are termed… (more)

Subjects/Keywords: gene delivery; polymers; liposomes

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Fitzsimmons, R. (2011). Novel polymer and lipid-based nanocarriers for gene delivery. (Masters Thesis). University of Alberta. Retrieved from https://era.library.ualberta.ca/files/gf06g346v

Chicago Manual of Style (16^th Edition):

Fitzsimmons, Ross. “Novel polymer and lipid-based nanocarriers for gene delivery.” 2011. Masters Thesis, University of Alberta. Accessed March 07, 2021. https://era.library.ualberta.ca/files/gf06g346v.

MLA Handbook (7^th Edition):

Fitzsimmons, Ross. “Novel polymer and lipid-based nanocarriers for gene delivery.” 2011. Web. 07 Mar 2021.

Vancouver:

Fitzsimmons R. Novel polymer and lipid-based nanocarriers for gene delivery. [Internet] [Masters thesis]. University of Alberta; 2011. [cited 2021 Mar 07]. Available from: https://era.library.ualberta.ca/files/gf06g346v.

Council of Science Editors:

Fitzsimmons R. Novel polymer and lipid-based nanocarriers for gene delivery. [Masters Thesis]. University of Alberta; 2011. Available from: https://era.library.ualberta.ca/files/gf06g346v

11. Παχής, Κωνσταντίνος. Ανάπτυξη λιποσωμικών μορφών νέων δραστικών μορίων για οφθαλμική χορήγηση.

Degree: 2012, University of Patras

URL: http://hdl.handle.net/10889/6056

►

Μελέτες έχουν δείξει ότι το νευροστεροειδές διυδροεπιανδροστερόνη (DHEA) προστατεύουν τους νευρώνες από ανοξία και απόπτωση. Έχει επίσης αποδειχθεί ότι το DHEA ενισχύει την κινητική δραστηριότητα… (more)

▼

Μελέτες έχουν δείξει ότι το νευροστεροειδές διυδροεπιανδροστερόνη (DHEA) προστατεύουν τους νευρώνες από ανοξία και απόπτωση. Έχει επίσης αποδειχθεί ότι το DHEA ενισχύει την κινητική δραστηριότητα πιθήκων με τη νόσο του Parkinson, ενώ επίσης σε συνδυασμό με την αλλοπρεγνανολόνη προκαλεί δημιουργία νευρώνων σε αρκετά πειραματικά μοντέλα. Τα λιποσώματα είναι σφαιρικά σωματίδια αποτελούμενα από ενυδατωμένες διπλοστοιβάδες φωσφολιπιδίων οι οποίες σχηματίζονται αυθόρμητα κατά τη διασπορά λιπιδίων στο νερό, εγκλωβίζοντας υδατικό διάλυμα στο εσωτερικό τους. Οι κυκλοδεξτρίνες είναι ολιγοσακχαρίτες με ειδική δομή κώνου, που έχουν υδρόφιλη πολική επιφάνεια και μη πολική εσωτερική κοιλότητα, έχουν τη δυνατότητα να ενσωματώνουν λιπόφιλα φάρμακα στην εσωτερική τους κοιλότητα (με τη δημιουργία συμπλόκων) και να αυξάνουν την υδατική διαλυτότητά τους. Στην παρούσα μελέτη χρησιμοποιήθηκαν τρία παράγωγα του νευροστεροειδούς DHEA, που έχουν τροποποιηθεί στις θέσεις C3 και C17 με σκοπό να βελτιώσουν την αντιαποπτωτική και νευροπροστατευτική δράση τους, καθώς και την αναστολή της μετατροπής τους σε ανδρογόνα και οιστρογόνα. Τα παράγωγα αυτά είναι εξαιρετικά λιπόφιλα και έχουν μικρό μοριακό βάρος και πολύ χαμηλή διαλυτότητα, γεγονός που καθιστά αδύνατη την χορήγησή τους. Για να αντιμετωπιστεί το πρόβλημα της χαμηλής διαλυτότητας, δοκιμάστηκαν δύο μέθοδοι: (1) H παρασκευή σύμπλοκων με υδροξυ-προπυλ-β-κυκλοδεξτρίνη (HP-beta-CD), και (2) Η ενσωμάτωσν των νευροστεροειδών στη λιπιδική μεμβράνη μικρών μονοστoιβαδιακών λιποσωμάτων (SUV) διαφόρων λιπιδικών συστάσεων. Μετά από τροποποίηση μιας ειδικής τεχνικής μέτρησης των στεροειδών, υπολογίστηκαν οι συγκετρώσεις που ενσωματώθηκαν στα λιποσώματα, καθώς και στα σύμπλοκα με τη κυκλοδεξτρίνη. Οι λιποσωμικές μορφές των παραγώγων δεν είχαν την αναμενόμενη ικανότητα ενσωμάτωσης τους, με αποτέλεσμα η συγκέντρωση των λιποσωμικών διαπορών να μην είναι αρκετά υψηλή για in vivo χορήγηση. Αντίθετα η HP-β-CD έδωσε σύμπλοκα που αύξησαν την υδατική διαλυτότητα των παραγώγων θεαματικά.Τα σύμπλοκα χαρακτηρίστηκαν με διάφορες τεχνικές, για να πιστωποιηθεί ο σχηματισμός τους. Με σκοπό να παρασκευαστούν μορφές με δυνατότητα ελεγχόμενης αποδέσμευσης των στεροειδών, μελετήθηκε στο επόμενο στάδιο της διατριβής, η ανάπτυξη υβριδικών μορφών που περιλαμβάνουν τον συνδυασμό συμπλόκων κυκλοδεξτρίνης και λιποσωμάτων, δηλαδή: λιποσώματα που στην εσωτερική τους κοιλότητα εγκλωβίζουν τα υδατοδιαλυτά σύμπλοκα των παραγώγων με κυκλοδεξτρίνες. Τα υβριδικά αυτά συστήματα είχαν αυξημένη ενσωμάτωση των νευροστεροειδών σε σύγκριση με τα συμβατικά λιποσώματα και κατάλληλα χαρακτηριστικά για in vivo χορήγηση.

Studies have shown that the neuroactive steroid dehydroepiandrosterone (DHEA) protects neurons from anoxia and apoptosis. It has also been demonstrated that DHEA is able to potentiate locomotor activity of hemi – Parkinsonian monkeys, and in conjunction with allopregnanolone can induce neurogenesis in various experimental models. Liposomes are spherical particles composed of hydrated bilayers of phospholipids which are…

Advisors/Committee Members: Αντιμησιάρη, Σοφία, Pachis, Konstantinos, Αντιμησιάρη, Σοφία, Κλεπετσάνης, Παύλος, Σωτηροπούλου, Γεωργία.

Subjects/Keywords: Λιποσώματα; Κυκλοδεξτρίνες; 615.78; Liposomes; Cyclodextrins

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Παχής, �. (2012). Ανάπτυξη λιποσωμικών μορφών νέων δραστικών μορίων για οφθαλμική χορήγηση. (Masters Thesis). University of Patras. Retrieved from http://hdl.handle.net/10889/6056

Chicago Manual of Style (16^th Edition):

Παχής, Κωνσταντίνος. “Ανάπτυξη λιποσωμικών μορφών νέων δραστικών μορίων για οφθαλμική χορήγηση.” 2012. Masters Thesis, University of Patras. Accessed March 07, 2021. http://hdl.handle.net/10889/6056.

MLA Handbook (7^th Edition):

Παχής, Κωνσταντίνος. “Ανάπτυξη λιποσωμικών μορφών νέων δραστικών μορίων για οφθαλμική χορήγηση.” 2012. Web. 07 Mar 2021.

Vancouver:

Παχής �. Ανάπτυξη λιποσωμικών μορφών νέων δραστικών μορίων για οφθαλμική χορήγηση. [Internet] [Masters thesis]. University of Patras; 2012. [cited 2021 Mar 07]. Available from: http://hdl.handle.net/10889/6056.

Council of Science Editors:

Παχής �. Ανάπτυξη λιποσωμικών μορφών νέων δραστικών μορίων για οφθαλμική χορήγηση. [Masters Thesis]. University of Patras; 2012. Available from: http://hdl.handle.net/10889/6056

Nelson Mandela Metropolitan University

12. Oidu, Benjamin. Uptake of liposomes into bacterial cells.

Degree: Faculty of Health Sciences, 2013, Nelson Mandela Metropolitan University

URL: http://hdl.handle.net/10948/d1021010

► Liposomes are small phospholipid vesicles that have been widely investigated as drug carriers for the delivery of therapeutic agents. A variety of liposome formulations are… (more)

Subjects/Keywords: Liposomes; Drug carriers (Pharmacy)

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Oidu, B. (2013). Uptake of liposomes into bacterial cells. (Thesis). Nelson Mandela Metropolitan University. Retrieved from http://hdl.handle.net/10948/d1021010

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Oidu, Benjamin. “Uptake of liposomes into bacterial cells.” 2013. Thesis, Nelson Mandela Metropolitan University. Accessed March 07, 2021. http://hdl.handle.net/10948/d1021010.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Oidu, Benjamin. “Uptake of liposomes into bacterial cells.” 2013. Web. 07 Mar 2021.

Vancouver:

Oidu B. Uptake of liposomes into bacterial cells. [Internet] [Thesis]. Nelson Mandela Metropolitan University; 2013. [cited 2021 Mar 07]. Available from: http://hdl.handle.net/10948/d1021010.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Oidu B. Uptake of liposomes into bacterial cells. [Thesis]. Nelson Mandela Metropolitan University; 2013. Available from: http://hdl.handle.net/10948/d1021010

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

13. Skouras, Athanasios. Ιn vitro διερεύνηση της ικανότητας στόχευσης του αιματεγκεφαλικού φραγμού και της αντικαρκινικής δράσης στοχευμένων πολυλειτουργικών μαγνητολιποσωμάτων που εγκλωβίζουν δοξορουβικίνη.

Degree: 2017, University of Patras; Πανεπιστήμιο Πατρών

URL: http://hdl.handle.net/10442/hedi/40646

► Magnetoliposomes can provide platforms for simultaneously carrying therapeutics and diagnostics. The controlled optical properties of the magnetic nanoparticles combined with the versatility of liposomes as… (more)

▼ Magnetoliposomes can provide platforms for simultaneously carrying therapeutics and diagnostics. The controlled optical properties of the magnetic nanoparticles combined with the versatility of liposomes as carriers can potentially enhance the efficacy of treatment for selective drug delivery by means of an external magnetically guided deposition and prolonged targeting by receptor-mediated activation (e.g., pegylation and surface modification: ligands and antibodies). Due to their optical properties, magnetic nanoparticles can serve as MRI contrast agents allowing their in vivo monitoring and distribution of the pharmaceuticals in a non-invasive way.The aim of this work was initially the formulation of magnetoliposomes, their physicochemical characterization and the impact of various factors on their physicochemical properties and stability.For the preparation of the MLs the initial target was the entrapment of the maximum concentration of USPIOs in small unilamellar vesicles, while retaining their size (<200nm) in order to exploit the effect of enhanced permeability and retention (EPR effect) presented by cancer cells. The DRV method (extruded), provided MLs with greater entrapment at the desired size. The addition of cholesterol to the ML had no significant effect on encapsulation, which applied also to the addition of negative charge (PG lipid), while addition of lipid PEG and higher initial ratios USPIOs / lipid increased the entrapment. Encapsulation of the USPIOs into liposomes led in a sharp increase of the magnetic response while higher Fe/Lipid ratio increased it even further. The same applied when 4mol% lipid-PEG was added or MLs size was increased, whereas addition of cholesterol reduced the response to the external magnetic field. USPIOs encapsulation also increased their ability as contrast agents, regardless lipid composition, addition of lipid PEG did not bring about any drastic change, while higher Fe/Lipid ratio led to an increase. MLs were stable long enough for use in in vivo applications and exhibited almost no cytotoxicity. Finally MLs were studied in vitro for their ability to cross the where it was found that in the presence of magnetic field, permeation increases up to 10 times, revealing the possibility offered by MLs to deliver drugs to the brain utilizing magnetic targeting.Next aim of this work was the formation of t-MLs for targeting the BBB. MLs decorated with either OX-26 antibody (targeting of the transferrin receptor) or the ApoE peptide (lipoprotein receptor targeting) or both were prepared and their effect on the physicochemical properties of MLs was studied. Decoration with one ligand had no significant effect on the entrapment or MLs’ size whilst a combination of 2 or 3 ligands lead to a slight decrease in the entrapment. Addition of an antibody on the MLs surface caused a slight reduction of the magnetophoretic ability of the MLs while this was the case also for their contrast agent ability. t-MLs were found to be stable for time sufficient enough for their administration and…

Subjects/Keywords: Μαγνητικά λιποσώματα; Magnetic liposomes

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Skouras, A. (2017). Ιn vitro διερεύνηση της ικανότητας στόχευσης του αιματεγκεφαλικού φραγμού και της αντικαρκινικής δράσης στοχευμένων πολυλειτουργικών μαγνητολιποσωμάτων που εγκλωβίζουν δοξορουβικίνη. (Thesis). University of Patras; Πανεπιστήμιο Πατρών. Retrieved from http://hdl.handle.net/10442/hedi/40646

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Skouras, Athanasios. “Ιn vitro διερεύνηση της ικανότητας στόχευσης του αιματεγκεφαλικού φραγμού και της αντικαρκινικής δράσης στοχευμένων πολυλειτουργικών μαγνητολιποσωμάτων που εγκλωβίζουν δοξορουβικίνη.” 2017. Thesis, University of Patras; Πανεπιστήμιο Πατρών. Accessed March 07, 2021. http://hdl.handle.net/10442/hedi/40646.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Skouras, Athanasios. “Ιn vitro διερεύνηση της ικανότητας στόχευσης του αιματεγκεφαλικού φραγμού και της αντικαρκινικής δράσης στοχευμένων πολυλειτουργικών μαγνητολιποσωμάτων που εγκλωβίζουν δοξορουβικίνη.” 2017. Web. 07 Mar 2021.

Vancouver:

Skouras A. Ιn vitro διερεύνηση της ικανότητας στόχευσης του αιματεγκεφαλικού φραγμού και της αντικαρκινικής δράσης στοχευμένων πολυλειτουργικών μαγνητολιποσωμάτων που εγκλωβίζουν δοξορουβικίνη. [Internet] [Thesis]. University of Patras; Πανεπιστήμιο Πατρών; 2017. [cited 2021 Mar 07]. Available from: http://hdl.handle.net/10442/hedi/40646.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Skouras A. Ιn vitro διερεύνηση της ικανότητας στόχευσης του αιματεγκεφαλικού φραγμού και της αντικαρκινικής δράσης στοχευμένων πολυλειτουργικών μαγνητολιποσωμάτων που εγκλωβίζουν δοξορουβικίνη. [Thesis]. University of Patras; Πανεπιστήμιο Πατρών; 2017. Available from: http://hdl.handle.net/10442/hedi/40646

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of KwaZulu-Natal

14. Narainpersad, Nicolisha. Mitochondrial localisation and cellular uptake in vitro using novel ‘mitochondriotropic’ liposomes.

Degree: 2016, University of KwaZulu-Natal

URL: http://hdl.handle.net/10413/16086

► Mitochondrial research has made tremendous strides since the 1980/90s when mitochondrial DNA mutations were first identified as a primary cause for human diseases and the… (more)

▼ Mitochondrial research has made tremendous strides since the 1980/90s when mitochondrial DNA mutations were first identified as a primary cause for human diseases and the organelle’s role in apoptosis was elucidated. These mutations of the mitochondrial genome have been implicated in a spectrum of clinical disorders especially involving the muscle and central nervous system. This makes the mitochondrion a prime candidate for organelle-specific delivery of exogenous materials such as therapeutic DNA and drugs, for therapy of diseases caused by mitochondrial dysfunction. However, reports of mitochondrial targeted delivery systems are limited. Hence vector design and development is of paramount importance. The success of liposomes viz. cationic liposomes, in chromosomal gene therapy make them potential vectors for mitochondrial gene targeting. In this investigation novel ‘mitochondriotropic’ liposomes were synthesised to evaluate their cellular uptake and mitochondrial localisation activity in vitro using four different mammalian cell culture models. Cationic cholesterol derivative, 3β [N-(N’,N’-dimethylaminopropane)-carbamoyl] cholesterol (CHOL-T) was formulated with dioleoylphosphatidylethanolamine (DOPE) to produce cationic liposomes, to which a mitochondrial targeting sequence (MTS) and octaarginine (R8) peptides were attached via two different novel cholesterol-derived cross-linking agents. Size, zeta potential, shape and lamellarity of liposomes and corresponding lipoplexes were assessed by the innovative technique, Nanoparticle Tracking Analysis (NTA) and cryogenic transmission electron microscopy. Their ability to bind, condense and protect plasmid DNA (pCMV-luc), was determined using the band shift, dye displacement and nuclease protection assays repectively. In vitro cytotoxicity and mechanism of cell death prompted by these novel liposomal preparations was determined using the MTT, AlamarBlue® and acridine orange and ethidium bromide (AO/EB) dual staining assays respectively, in the hepatocyte-derived human cell line (HepG2), human embryonic kidney cells (HEK293), the human intestinal cell line (Caco-2) and human cervical carcinoma (HeLa-Tat luc) cells. Fluorescently labelled DNA was used to determine cellular uptake and mitochondrial targeting and localisation ability of these cationic mitochondriotropic liposomal formulations in the target organelles, mitochondria using fluorescence microscopy and the quantitative evaluation of fluorescence in the mitochondrial fraction of cell homogenate cocktails. These mitochondriotropic liposomes successfully bind, condense and protect plasmid DNA in the presence of serum, are fairly well tolerated by all cell lines tested in culture with cell death observed to be apoptotic and not necrotic in nature. The liposomes were shown to successfully enhance cellular uptake in all cell culture models tested. Furthermore, results demonstrate positive mitochondrial targeting and localisation activity facilitated by the presence of MTS peptide and a combination of MTS and R8… Advisors/Committee Members: Ariatti, Mario. (advisor), Masola, Bubuya. (advisor), Singh, Moganavelli. (advisor).

Subjects/Keywords: Cytotoxicity.; Targeting.; Vitro.; Mitochondriotropic.; Liposomes.

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Narainpersad, N. (2016). Mitochondrial localisation and cellular uptake in vitro using novel ‘mitochondriotropic’ liposomes. (Thesis). University of KwaZulu-Natal. Retrieved from http://hdl.handle.net/10413/16086

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Narainpersad, Nicolisha. “Mitochondrial localisation and cellular uptake in vitro using novel ‘mitochondriotropic’ liposomes.” 2016. Thesis, University of KwaZulu-Natal. Accessed March 07, 2021. http://hdl.handle.net/10413/16086.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Narainpersad, Nicolisha. “Mitochondrial localisation and cellular uptake in vitro using novel ‘mitochondriotropic’ liposomes.” 2016. Web. 07 Mar 2021.

Vancouver:

Narainpersad N. Mitochondrial localisation and cellular uptake in vitro using novel ‘mitochondriotropic’ liposomes. [Internet] [Thesis]. University of KwaZulu-Natal; 2016. [cited 2021 Mar 07]. Available from: http://hdl.handle.net/10413/16086.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Narainpersad N. Mitochondrial localisation and cellular uptake in vitro using novel ‘mitochondriotropic’ liposomes. [Thesis]. University of KwaZulu-Natal; 2016. Available from: http://hdl.handle.net/10413/16086

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Nelson Mandela Metropolitan University

15. [No author]. Development of a liposomal acyclovir mucoadhesive film.

Degree: Faculty of Science, 2017, Nelson Mandela Metropolitan University

URL: http://hdl.handle.net/10948/14212

► Acyclovir is a synthetic purine nucleoside analogue with in vitro and in vivo inhibitory activity against herpes simplex virus types 1 (HSV-1), 2 (HSV-2), and… (more)

▼ Acyclovir is a synthetic purine nucleoside analogue with in vitro and in vivo inhibitory activity against herpes simplex virus types 1 (HSV-1), 2 (HSV-2), and varicella-zoster virus (VZV). The efficacy of oral acyclovir is limited as a result of its low bioavailability (15-30%) as it is poorly water soluble and therefore requires a frequent dosing regimen. When orally administered, peak plasma concentration occurs after 1.5–2.5 hours, while its elimination half-life is approximately 2-3 hours. Acyclovir displays poor solubility in water and in lipid bilayers, which leads to poor drug levels at target sites after oral, local, or parenteral administration. In order to improve this lack of solubility, novel amphiphilic derivatives have been designed to form nanoparticles, which allow for the efficient encapsulation of this hydrophobic antiviral agent. Reformulation of drugs in liposomes has provided an opportunity to enhance the therapeutic indices of various agents mainly via alteration of their bio-distribution. Liposomal drug delivery systems have received considerable attention due to their immense advantages which include, effective encapsulation of both small and large molecules that have a wide range of hydrophobicity levels and pKa values, prolonging and targeting release of therapeutic agents by modification of liposomal surface and also minimising clinical drug dose thus reducing toxicity effects. Liposomes exhibit a number of special biological characteristics, including specific interactions with biological membranes and various cells, hence, liposomes are used as biocompatible carriers to improve delivery properties across mucus membranes. Mucoadhesive dosage forms may be designed to enable prolonged retention at the site of application, providing a controlled rate of drug release for improved therapeutic outcome. The aim of this study was to develop an acyclovir liposomal mucoadhesive film by actively encapsulating acyclovir into liposomes and preparing a mucoadhesive film to optimise delivery of acyclovir liposomes at target sites. To actively encapsulate acyclovir and prepare the acyclovir-containing liposomes, a comprehensive statistical methodology was used in optimising the liposome formulation to encapsulate acyclovir. Central composite design was used as the response surface methodology statistical tool to design and develop an optimised method for active encapsulation of acyclovir into liposomes. The predicted optimised encapsulation parameters were incubation temperature of 60 °C and incubation time of 45 minutes. The mean percentage encapsulation calculated was 27.72%. The overall average size of the liposomes was 99.5 nm with a narrow distribution polydispersity index of 0.105 and were physically characterised as small unilamellar vesicles which possessed an average zeta potential of -45.6 mV. High Performance Liquid Chromatography (HPLC) was used to analyse and determine acyclovir drug content in the liposomes and drug release pattern from the mucoadhesive film. Polyvinyl-pyrrolidone (PVP) and…

Subjects/Keywords: Clinical pharmacology; Liposomes; Nanomedicine

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APA (6^th Edition):

author], [. (2017). Development of a liposomal acyclovir mucoadhesive film. (Thesis). Nelson Mandela Metropolitan University. Retrieved from http://hdl.handle.net/10948/14212

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

author], [No. “Development of a liposomal acyclovir mucoadhesive film.” 2017. Thesis, Nelson Mandela Metropolitan University. Accessed March 07, 2021. http://hdl.handle.net/10948/14212.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

author], [No. “Development of a liposomal acyclovir mucoadhesive film.” 2017. Web. 07 Mar 2021.

Vancouver:

author] [. Development of a liposomal acyclovir mucoadhesive film. [Internet] [Thesis]. Nelson Mandela Metropolitan University; 2017. [cited 2021 Mar 07]. Available from: http://hdl.handle.net/10948/14212.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

author] [. Development of a liposomal acyclovir mucoadhesive film. [Thesis]. Nelson Mandela Metropolitan University; 2017. Available from: http://hdl.handle.net/10948/14212

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Adelaide

16. Zhang, Mengjue. Microfluidic system development for drug delivery.

Degree: 2016, University of Adelaide

URL: http://hdl.handle.net/2440/102614

► Development and application of a microfluidic system for generating drug delivery carriers are investigated in this research. Various types of microfluidic devices are designed and… (more)

▼ Development and application of a microfluidic system for generating drug delivery carriers are investigated in this research. Various types of microfluidic devices are designed and fabricated for peptide nanotubes, liposome vesicles and double emulsions formation. The microfluidic system offers a better control over the formation process of all three drug delivery carriers. Comparing to traditional methods such as bulk mixing, the process efficiency, size and size distribution of the final products are significantly improved. The results generated show that tuning the flow rate ratios between different reagents from the inlet streams successfully controls the sizes and size distributions of liposomes vesicles. The relationship between the flow rate ratio and the size of the resulting vesicles is established. Macrocycle (AP-169) that was found to self-assemble into an anti-parallel β-sheet nanotube with a triggering agent is successfully synthesized and purified for peptide nanotube self-assembling process. A microfluidic device is designed and fabricated to control the interaction between AP-169 and its self-assembling triggering agent, dimethyl sulfoxide. Double emulsions with different radii are produced with the microfluidic system by adjusting the flow rate ratio between each phase of the solution, and changing the wetting properties of the microchannels. The stability of double emulsions is enhanced by introducing various surfactants. The sizes and size distributions of liposomes and double emulsions have been successfully controlled and optimized for drug delivery. In conclusion, various drug delivery carriers have been successfully generated and optimized with a designed and modified microfluidic system. These products can be further applied in drug encapsulation, biomolecular screening and in vitro compartmentalization in the future. Advisors/Committee Members: Bi, Jingxiu (advisor), Zhang, Hu (advisor), Biggs, Mark James (advisor), School of Chemical Engineering (school).

Subjects/Keywords: microfluidic; liposomes; double emulsions

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APA (6^th Edition):

Zhang, M. (2016). Microfluidic system development for drug delivery. (Thesis). University of Adelaide. Retrieved from http://hdl.handle.net/2440/102614

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Zhang, Mengjue. “Microfluidic system development for drug delivery.” 2016. Thesis, University of Adelaide. Accessed March 07, 2021. http://hdl.handle.net/2440/102614.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Zhang, Mengjue. “Microfluidic system development for drug delivery.” 2016. Web. 07 Mar 2021.

Vancouver:

Zhang M. Microfluidic system development for drug delivery. [Internet] [Thesis]. University of Adelaide; 2016. [cited 2021 Mar 07]. Available from: http://hdl.handle.net/2440/102614.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Zhang M. Microfluidic system development for drug delivery. [Thesis]. University of Adelaide; 2016. Available from: http://hdl.handle.net/2440/102614

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Deakin University

17. Squire, Jennifer Suzanne. Synthesis and application of self-assembled amphiphiles towards novel drug delivery.

Degree: School of Life and Environmental Sciences, 2013, Deakin University

URL: http://hdl.handle.net/10536/DRO/DU:30063487

A library of novel amphiphiles were designed and synthesised, towards self-assembly based drug delivery for the treatment of breast cancer. The amphiphiles were formulated into liposomes of varying size and shape, and showed promising potential for drug delivery. Active targeting of breast cancer tumours was also explored with promising results.

Subjects/Keywords: Breast cancer; Novel amphiphiles; Liposomes

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APA (6^th Edition):

Squire, J. S. (2013). Synthesis and application of self-assembled amphiphiles towards novel drug delivery. (Thesis). Deakin University. Retrieved from http://hdl.handle.net/10536/DRO/DU:30063487

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Squire, Jennifer Suzanne. “Synthesis and application of self-assembled amphiphiles towards novel drug delivery.” 2013. Thesis, Deakin University. Accessed March 07, 2021. http://hdl.handle.net/10536/DRO/DU:30063487.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Squire, Jennifer Suzanne. “Synthesis and application of self-assembled amphiphiles towards novel drug delivery.” 2013. Web. 07 Mar 2021.

Vancouver:

Squire JS. Synthesis and application of self-assembled amphiphiles towards novel drug delivery. [Internet] [Thesis]. Deakin University; 2013. [cited 2021 Mar 07]. Available from: http://hdl.handle.net/10536/DRO/DU:30063487.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Squire JS. Synthesis and application of self-assembled amphiphiles towards novel drug delivery. [Thesis]. Deakin University; 2013. Available from: http://hdl.handle.net/10536/DRO/DU:30063487

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Boston University

18. Kottapalli, Sai M. Effect of macrophage depletion on asthmatic responses in a cockroach allergen induced murine model.

Degree: 2014, Boston University

URL: http://hdl.handle.net/2144/13304

► Asthma is a chronic obstructive pulmonary disease (COPD) which affects 1 in every 12 Americans. Symptoms common to asthmatics include dyspnea, increased mucous production and… (more)

▼ Asthma is a chronic obstructive pulmonary disease (COPD) which affects 1 in every 12 Americans. Symptoms common to asthmatics include dyspnea, increased mucous production and airway hyperresponsiveness. While research over the past few decades has mostly established the immunological basis behind asthma, there have not been radical changes in the treatment modalities. It is believed that in many COPDs, alveolar macrophages play a critical role in disease progression. While evolutionarily, alveolar macrophages played a significant part in protecting the individual from harmful allergens, in asthma there may be an inappropriate activation of the alveolar macrophages to proteases such as cockroach allergen (CRA). Studies show that children living in inner cities with cockroach infestation are more likely to develop asthma than those that reside in rural areas with less exposure to cockroach allergens. In exposed individuals, when the alveolar macrophages come in contact with CRA, an immune cascade is initiated which sensitizes the child. Subsequent exposure to such an antigen will induce asthma like symptoms. One possible way of reducing such a response is to reduce the number of alveolar macrophages thus avoiding the pathalogical effects. Clodronate liposomes are liposomes that are encapsulated with bisphosphonate clodronate. When a macrophage phagocytoses such a liposome, the result is cellular suicide or apoptosis. In this study, we sensitized a murine model of CRA asthma and then monitored the impact of depleted alveolar macrophages using intratracheal administration of clodronate liposomes. We then studied the effect of this depletion on the recruitment of inflammatory cells such as neutrophils and eosinophils which are primary cellular contributors to the asthmatic response. Our studies show that while clodronate liposomes are effective in alveolar macrophage depletion, the subsequent inflammation through neutrophil recruitment interferes with the study of the delicate milieu of cells in the respiratory epithelium of this murine model.

Subjects/Keywords: Immunology; Asthma; Clodronate liposomes

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APA (6^th Edition):

Kottapalli, S. M. (2014). Effect of macrophage depletion on asthmatic responses in a cockroach allergen induced murine model. (Thesis). Boston University. Retrieved from http://hdl.handle.net/2144/13304

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Kottapalli, Sai M. “Effect of macrophage depletion on asthmatic responses in a cockroach allergen induced murine model.” 2014. Thesis, Boston University. Accessed March 07, 2021. http://hdl.handle.net/2144/13304.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Kottapalli, Sai M. “Effect of macrophage depletion on asthmatic responses in a cockroach allergen induced murine model.” 2014. Web. 07 Mar 2021.

Vancouver:

Kottapalli SM. Effect of macrophage depletion on asthmatic responses in a cockroach allergen induced murine model. [Internet] [Thesis]. Boston University; 2014. [cited 2021 Mar 07]. Available from: http://hdl.handle.net/2144/13304.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Kottapalli SM. Effect of macrophage depletion on asthmatic responses in a cockroach allergen induced murine model. [Thesis]. Boston University; 2014. Available from: http://hdl.handle.net/2144/13304

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Michigan State University

19. Knudson, Kristine Callenbach, 1945-. Studies on the human and guinea pig serum complement systems with liposomal model membranes.

Degree: PhD, Department of Microbiology and Public Health, 1971, Michigan State University

URL: http://etd.lib.msu.edu/islandora/object/etd:23697

Subjects/Keywords: Serum; Liposomes

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APA (6^th Edition):

Knudson, Kristine Callenbach, 1. (1971). Studies on the human and guinea pig serum complement systems with liposomal model membranes. (Doctoral Dissertation). Michigan State University. Retrieved from http://etd.lib.msu.edu/islandora/object/etd:23697

Chicago Manual of Style (16^th Edition):

Knudson, Kristine Callenbach, 1945-. “Studies on the human and guinea pig serum complement systems with liposomal model membranes.” 1971. Doctoral Dissertation, Michigan State University. Accessed March 07, 2021. http://etd.lib.msu.edu/islandora/object/etd:23697.

MLA Handbook (7^th Edition):

Knudson, Kristine Callenbach, 1945-. “Studies on the human and guinea pig serum complement systems with liposomal model membranes.” 1971. Web. 07 Mar 2021.

Vancouver:

Knudson, Kristine Callenbach 1. Studies on the human and guinea pig serum complement systems with liposomal model membranes. [Internet] [Doctoral dissertation]. Michigan State University; 1971. [cited 2021 Mar 07]. Available from: http://etd.lib.msu.edu/islandora/object/etd:23697.

Council of Science Editors:

Knudson, Kristine Callenbach 1. Studies on the human and guinea pig serum complement systems with liposomal model membranes. [Doctoral Dissertation]. Michigan State University; 1971. Available from: http://etd.lib.msu.edu/islandora/object/etd:23697

Michigan State University

20. Callow, Richard. Thermodynamic modelling and cryomicroscopy of large unilamellar liposomes.

Degree: MS, Department of Mechanical Engineering, 1983, Michigan State University

URL: http://etd.lib.msu.edu/islandora/object/etd:46264

Subjects/Keywords: Liposomes; Cryobiology

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APA (6^th Edition):

Callow, R. (1983). Thermodynamic modelling and cryomicroscopy of large unilamellar liposomes. (Masters Thesis). Michigan State University. Retrieved from http://etd.lib.msu.edu/islandora/object/etd:46264

Chicago Manual of Style (16^th Edition):

Callow, Richard. “Thermodynamic modelling and cryomicroscopy of large unilamellar liposomes.” 1983. Masters Thesis, Michigan State University. Accessed March 07, 2021. http://etd.lib.msu.edu/islandora/object/etd:46264.

MLA Handbook (7^th Edition):

Callow, Richard. “Thermodynamic modelling and cryomicroscopy of large unilamellar liposomes.” 1983. Web. 07 Mar 2021.

Vancouver:

Callow R. Thermodynamic modelling and cryomicroscopy of large unilamellar liposomes. [Internet] [Masters thesis]. Michigan State University; 1983. [cited 2021 Mar 07]. Available from: http://etd.lib.msu.edu/islandora/object/etd:46264.

Council of Science Editors:

Callow R. Thermodynamic modelling and cryomicroscopy of large unilamellar liposomes. [Masters Thesis]. Michigan State University; 1983. Available from: http://etd.lib.msu.edu/islandora/object/etd:46264

Boston University

21. Rubio, Angel Jonathan. Designing chemokine-loaded temperature-sensitive liposomes.

Degree: PhD, Pharmacology, 2020, Boston University

URL: http://hdl.handle.net/2144/39426

► Surgery, radiation, and chemotherapy have been the primary options for cancer patients; however, the recent emergence of immunotherapy has revolutionized the cancer treatment paradigm. Cancer… (more)

Subjects/Keywords: Pharmacology; B cells; Chemokine; Liposomes

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APA (6^th Edition):

Rubio, A. J. (2020). Designing chemokine-loaded temperature-sensitive liposomes. (Doctoral Dissertation). Boston University. Retrieved from http://hdl.handle.net/2144/39426

Chicago Manual of Style (16^th Edition):

Rubio, Angel Jonathan. “Designing chemokine-loaded temperature-sensitive liposomes.” 2020. Doctoral Dissertation, Boston University. Accessed March 07, 2021. http://hdl.handle.net/2144/39426.

MLA Handbook (7^th Edition):

Rubio, Angel Jonathan. “Designing chemokine-loaded temperature-sensitive liposomes.” 2020. Web. 07 Mar 2021.

Vancouver:

Rubio AJ. Designing chemokine-loaded temperature-sensitive liposomes. [Internet] [Doctoral dissertation]. Boston University; 2020. [cited 2021 Mar 07]. Available from: http://hdl.handle.net/2144/39426.

Council of Science Editors:

Rubio AJ. Designing chemokine-loaded temperature-sensitive liposomes. [Doctoral Dissertation]. Boston University; 2020. Available from: http://hdl.handle.net/2144/39426

Laurentian University

22. Alhariri, Moayad Abdulaziz I. Evaluation of liposomal bismuth-ethanedithiol-tobramycin for treatment of cystic fibrosis pulmonary pseudomonas aeruginosa infection .

Degree: 2013, Laurentian University

URL: https://zone.biblio.laurentian.ca/dspace/handle/10219/2099

► The effectiveness of liposomes incorporating bismuth-ethanedithiol and loaded with tobramycin (LipoBiEDT-TOB) at sub-inhibitory concentrations to inhibit the production of quorum sensing signaling molecules and virulence… (more)

Subjects/Keywords: Cystic fibrosis; Liposomes; Aminoglycosides; Bacteria

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APA (6^th Edition):

Alhariri, M. A. I. (2013). Evaluation of liposomal bismuth-ethanedithiol-tobramycin for treatment of cystic fibrosis pulmonary pseudomonas aeruginosa infection . (Thesis). Laurentian University. Retrieved from https://zone.biblio.laurentian.ca/dspace/handle/10219/2099

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Alhariri, Moayad Abdulaziz I. “Evaluation of liposomal bismuth-ethanedithiol-tobramycin for treatment of cystic fibrosis pulmonary pseudomonas aeruginosa infection .” 2013. Thesis, Laurentian University. Accessed March 07, 2021. https://zone.biblio.laurentian.ca/dspace/handle/10219/2099.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Alhariri, Moayad Abdulaziz I. “Evaluation of liposomal bismuth-ethanedithiol-tobramycin for treatment of cystic fibrosis pulmonary pseudomonas aeruginosa infection .” 2013. Web. 07 Mar 2021.

Vancouver:

Alhariri MAI. Evaluation of liposomal bismuth-ethanedithiol-tobramycin for treatment of cystic fibrosis pulmonary pseudomonas aeruginosa infection . [Internet] [Thesis]. Laurentian University; 2013. [cited 2021 Mar 07]. Available from: https://zone.biblio.laurentian.ca/dspace/handle/10219/2099.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Alhariri MAI. Evaluation of liposomal bismuth-ethanedithiol-tobramycin for treatment of cystic fibrosis pulmonary pseudomonas aeruginosa infection . [Thesis]. Laurentian University; 2013. Available from: https://zone.biblio.laurentian.ca/dspace/handle/10219/2099

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Louisiana State University

23. Carrier, Nicole Hollabaugh. Redox-active Liposome Delivery Agents With Highly Controllable Stimuli-responsive Behavior.

Degree: PhD, Chemistry, 2011, Louisiana State University

URL: etd-06232011-143040 ; https://digitalcommons.lsu.edu/gradschool_dissertations/1147

► Over the last few decades, liposomes have generated a lot of interest as drug delivery vehicles to address the need for providing both increased therapeutic… (more)

▼ Over the last few decades, liposomes have generated a lot of interest as drug delivery vehicles to address the need for providing both increased therapeutic efficacy and decreased systemic exposure, simultaneously. The challenge of increasing drug accumulation at diseased sites, without compromising the integrity and stability of the liposomal carrier during circulation, has been met with two possible solutions: (1) active targeting and (2) active triggering. To achieve selective and site-specific delivery of drugs to tumors, active triggering methods have been developed wherein a responsive element is incorporated into the liposomal bilayer, which causes destabilization of the liposome upon exposure to the proper stimulus. Endogenous stimuli can offer high specificities and sensitivities, if appropriate trigger groups exist so as to take full advantage of diseased site characteristics. The research described herein involved the synthesis of redox-active, quinone-lipid conjugates that were prepared into liposomes for the containment and subsequent triggered release of encapsulated cargo. Development of said system required (1) the synthesis and then characterization of various simple quinones and quinone propionic acids by cyclic voltammetry and X-ray crystallography and (2) the preparation of substituted quinone dioleoylphosphatidylethanolamine lipids into liposomes and evaluation of their triggered release behaviors by fluorescence emission spectrometry. Elucidation of the electrochemical properties of simple quinones and quinone propionic acids revealed a correlation between quinone substitution and reduction potential, meaning that the electronics of the quinone trigger can be adjusted through their chemical structure. X-ray crystallography showed a highly distorted quinone ring proximal to the trimethyl-locked propionic acid side chain. Upon introduction of a chemical reducing agent, the four different substituted quinone-dioleoylphosphatidylethanolamine liposomes each displayed distinctive release behaviors, as indicated by the time-dependent increase in fluorescence signal as encapsulated calcein was released below its self-quenching concentration. The individual release profiles demonstrate the influence of quinone substitution on the triggered response of these redox-active liposomes; thus, realizing the programmed delivery of liposomal contents through active triggering. The information learned from this research project provides a solid foundation for exploring the triggered release of these redox-active liposomes by NAD(P)H:quinone acceptor oxidoreductase type 1, an over-expressed reductase enzyme in numerous cancer cell lines.

Subjects/Keywords: liposomes; quinones; redox; drug delivery

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APA (6^th Edition):

Carrier, N. H. (2011). Redox-active Liposome Delivery Agents With Highly Controllable Stimuli-responsive Behavior. (Doctoral Dissertation). Louisiana State University. Retrieved from etd-06232011-143040 ; https://digitalcommons.lsu.edu/gradschool_dissertations/1147

Chicago Manual of Style (16^th Edition):

Carrier, Nicole Hollabaugh. “Redox-active Liposome Delivery Agents With Highly Controllable Stimuli-responsive Behavior.” 2011. Doctoral Dissertation, Louisiana State University. Accessed March 07, 2021. etd-06232011-143040 ; https://digitalcommons.lsu.edu/gradschool_dissertations/1147.

MLA Handbook (7^th Edition):

Carrier, Nicole Hollabaugh. “Redox-active Liposome Delivery Agents With Highly Controllable Stimuli-responsive Behavior.” 2011. Web. 07 Mar 2021.

Vancouver:

Carrier NH. Redox-active Liposome Delivery Agents With Highly Controllable Stimuli-responsive Behavior. [Internet] [Doctoral dissertation]. Louisiana State University; 2011. [cited 2021 Mar 07]. Available from: etd-06232011-143040 ; https://digitalcommons.lsu.edu/gradschool_dissertations/1147.

Council of Science Editors:

Carrier NH. Redox-active Liposome Delivery Agents With Highly Controllable Stimuli-responsive Behavior. [Doctoral Dissertation]. Louisiana State University; 2011. Available from: etd-06232011-143040 ; https://digitalcommons.lsu.edu/gradschool_dissertations/1147

Rutgers University

24. Rane, Varsha, 1991-. pH sensitive liposomes for targeted delivery of antibiotics to localized internal bacterial infection sites.

Degree: MS, Chemical and Biochemical Engineering, 2016, Rutgers University

URL: https://rucore.libraries.rutgers.edu/rutgers-lib/49282/

► Methicillin Resistant Staphylococcus aureus (MRSA) causes a myriad of infections ranging from mild skin-infection to more serious infections affecting internal organs. A glycopeptide, Vancomycin, remains… (more)

Subjects/Keywords: Liposomes; Antibiotics; Drug delivery systems

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APA (6^th Edition):

Rane, Varsha, 1. (2016). pH sensitive liposomes for targeted delivery of antibiotics to localized internal bacterial infection sites. (Masters Thesis). Rutgers University. Retrieved from https://rucore.libraries.rutgers.edu/rutgers-lib/49282/

Chicago Manual of Style (16^th Edition):

Rane, Varsha, 1991-. “pH sensitive liposomes for targeted delivery of antibiotics to localized internal bacterial infection sites.” 2016. Masters Thesis, Rutgers University. Accessed March 07, 2021. https://rucore.libraries.rutgers.edu/rutgers-lib/49282/.

MLA Handbook (7^th Edition):

Rane, Varsha, 1991-. “pH sensitive liposomes for targeted delivery of antibiotics to localized internal bacterial infection sites.” 2016. Web. 07 Mar 2021.

Vancouver:

Rane, Varsha 1. pH sensitive liposomes for targeted delivery of antibiotics to localized internal bacterial infection sites. [Internet] [Masters thesis]. Rutgers University; 2016. [cited 2021 Mar 07]. Available from: https://rucore.libraries.rutgers.edu/rutgers-lib/49282/.

Council of Science Editors:

Rane, Varsha 1. pH sensitive liposomes for targeted delivery of antibiotics to localized internal bacterial infection sites. [Masters Thesis]. Rutgers University; 2016. Available from: https://rucore.libraries.rutgers.edu/rutgers-lib/49282/

Rutgers University

25. Varghese, Nevin. Characterization of the interaction of daptomycin with bacterial liposomal analogues.

Degree: MS, Biomedical Engineering, 2016, Rutgers University

URL: https://rucore.libraries.rutgers.edu/rutgers-lib/51486/

► Efforts to curb the prevalence of antibiotic resistant bacteria are unable to keep up with the aggressive adaptation of these bacterial species to existing antibiotics.… (more)

Subjects/Keywords: Liposomes; Drug resistance in microorganisms

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APA (6^th Edition):

Varghese, N. (2016). Characterization of the interaction of daptomycin with bacterial liposomal analogues. (Masters Thesis). Rutgers University. Retrieved from https://rucore.libraries.rutgers.edu/rutgers-lib/51486/

Chicago Manual of Style (16^th Edition):

Varghese, Nevin. “Characterization of the interaction of daptomycin with bacterial liposomal analogues.” 2016. Masters Thesis, Rutgers University. Accessed March 07, 2021. https://rucore.libraries.rutgers.edu/rutgers-lib/51486/.

MLA Handbook (7^th Edition):

Varghese, Nevin. “Characterization of the interaction of daptomycin with bacterial liposomal analogues.” 2016. Web. 07 Mar 2021.

Vancouver:

Varghese N. Characterization of the interaction of daptomycin with bacterial liposomal analogues. [Internet] [Masters thesis]. Rutgers University; 2016. [cited 2021 Mar 07]. Available from: https://rucore.libraries.rutgers.edu/rutgers-lib/51486/.

Council of Science Editors:

Varghese N. Characterization of the interaction of daptomycin with bacterial liposomal analogues. [Masters Thesis]. Rutgers University; 2016. Available from: https://rucore.libraries.rutgers.edu/rutgers-lib/51486/

Rutgers University

26. Stras, Sally. Environmentally responsive liposomes for treatment of metastatic triple negative breast cancer.

Degree: PhD, Chemical and Biochemical Engineering, 2018, Rutgers University

URL: https://rucore.libraries.rutgers.edu/rutgers-lib/57727/

►

In 2015, it was estimated that approximately 1.7 million women were diagnosed with breast cancer in the United States alone. Breast cancer is the most… (more)

▼

In 2015, it was estimated that approximately 1.7 million women were diagnosed with breast cancer in the United States alone. Breast cancer is the most common cancer among women. One in eight women in their lifetime will have to endure the tribulations of discovering that they have breast cancer and all that comes in hopes of beating the disease. Triple-negative breast cancer (TNBC) is a subgroup of breast cancer associated with a poor prognosis and a higher chance of cancer metastasis outside the breast. TNBC is defined as being negative in gene expression for estrogen receptor (ER), progesterone receptor (PR) and lacking gene expression of HER2/neu. Current methods for targeting TNBC tumors remain in investigative stages due to the difficulty in discovering an appropriate and direct method of targeting. To enable selective and effective treatment of TNBC solid tumors, we study a drug delivery carrier of cisplatin (CDDP) - a clinically accepted major line of therapy for TNBC - that is designed to ultimately result in (a) deep penetration and homogeneous distribution of the drug within tumors and (b) enhanced ii uptake by the cancer cells that constitute these tumors. Towards these aims, we engineered a carrier that is a tunable (pH-sensitive) liposome encapsulating cisplatin. These liposomes are designed to form lipid-phase separated domains at acidic pH. Domain formation is tuned to trigger content release, and the change is pH is used to increase the adsorptive/adhesive property of these liposomes. Improved tumor penetration of delivered cisplatin is expected to be achieved by triggered release of cisplatin directly within the tumor interstitial region from extravasated liposomes. Further increase in intracellularly delivered cisplatin (due to more favorable retention of liposomes by solid tumors in vivo) is expected to be achieved by liposomes that are functionalized with an adhesive switch on their surface with the aim to slow their clearance from the tumor.

Advisors/Committee Members: Sofou, Stavroula (chair), School of Graduate Studies.

Subjects/Keywords: Breast – Cancer – Treatment; Liposomes

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Stras, S. (2018). Environmentally responsive liposomes for treatment of metastatic triple negative breast cancer. (Doctoral Dissertation). Rutgers University. Retrieved from https://rucore.libraries.rutgers.edu/rutgers-lib/57727/

Chicago Manual of Style (16^th Edition):

Stras, Sally. “Environmentally responsive liposomes for treatment of metastatic triple negative breast cancer.” 2018. Doctoral Dissertation, Rutgers University. Accessed March 07, 2021. https://rucore.libraries.rutgers.edu/rutgers-lib/57727/.

MLA Handbook (7^th Edition):

Stras, Sally. “Environmentally responsive liposomes for treatment of metastatic triple negative breast cancer.” 2018. Web. 07 Mar 2021.

Vancouver:

Stras S. Environmentally responsive liposomes for treatment of metastatic triple negative breast cancer. [Internet] [Doctoral dissertation]. Rutgers University; 2018. [cited 2021 Mar 07]. Available from: https://rucore.libraries.rutgers.edu/rutgers-lib/57727/.

Council of Science Editors:

Stras S. Environmentally responsive liposomes for treatment of metastatic triple negative breast cancer. [Doctoral Dissertation]. Rutgers University; 2018. Available from: https://rucore.libraries.rutgers.edu/rutgers-lib/57727/

Rutgers University

27. Shah, Shiv S. Transdermal delivery of insulin: an application of pH sensitive liposomes.

Degree: MS, Chemical and Biochemical Engineering, 2015, Rutgers University

URL: https://rucore.libraries.rutgers.edu/rutgers-lib/47586/

► Diabetes is a disease where the body either cannot produce insulin (type I) or the cells simply reject the insulin (type II). While type II… (more)

Subjects/Keywords: Transdermal medication; Insulin; Liposomes

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Shah, S. S. (2015). Transdermal delivery of insulin: an application of pH sensitive liposomes. (Masters Thesis). Rutgers University. Retrieved from https://rucore.libraries.rutgers.edu/rutgers-lib/47586/

Chicago Manual of Style (16^th Edition):

Shah, Shiv S. “Transdermal delivery of insulin: an application of pH sensitive liposomes.” 2015. Masters Thesis, Rutgers University. Accessed March 07, 2021. https://rucore.libraries.rutgers.edu/rutgers-lib/47586/.

MLA Handbook (7^th Edition):

Shah, Shiv S. “Transdermal delivery of insulin: an application of pH sensitive liposomes.” 2015. Web. 07 Mar 2021.

Vancouver:

Shah SS. Transdermal delivery of insulin: an application of pH sensitive liposomes. [Internet] [Masters thesis]. Rutgers University; 2015. [cited 2021 Mar 07]. Available from: https://rucore.libraries.rutgers.edu/rutgers-lib/47586/.

Council of Science Editors:

Shah SS. Transdermal delivery of insulin: an application of pH sensitive liposomes. [Masters Thesis]. Rutgers University; 2015. Available from: https://rucore.libraries.rutgers.edu/rutgers-lib/47586/

28. Demazeau, Maxime. Relations structure-effet de nanovecteurs à base de copolymères à blocs pour la thérapie photodynamique : utilisation de modèles de membranes : Structure-effect relationship of bloc copolymer based nanocarriers for photodynamic therapy : the use of model membrane.

Degree: Docteur es, Chimie, Biologie, Santé, 2019, Université Toulouse III – Paul Sabatier

URL: http://www.theses.fr/2019TOU30113

►

La thérapie photodynamique (PDT), une thérapie basée sur l'irradiation de molécules photosensibilisatrices pour générer un stress oxydant, est déjà utilisée comme traitement de certaines pathologies.… (more)

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La thérapie photodynamique (PDT), une thérapie basée sur l'irradiation de molécules photosensibilisatrices pour générer un stress oxydant, est déjà utilisée comme traitement de certaines pathologies. Très souvent, les photosensibilisateurs utilisés sont des molécules fortement hydrophobes qui s'agrègent en milieux aqueux. De ce fait, utilisées seules, elles nécessitent d'être injectées à des concentrations élevées, entraînant un risque de photosensibilisation générale. Pour diminuer cet effet secondaire et rendre le traitement plus efficace, il est possible d'encapsuler ces molécules. Des travaux précédant au sein du laboratoire des IRMCP ont permis le développement de vecteurs à base de copolymères à blocs pour l'encapsulation d'un photosensibilisateur, le phéophorbide-a. Ces travaux ont montré une efficacité de certains de ces vecteurs en conditions PDT sur des cultures cellulaires et ont ouvert des questions sur les processus de réponse cellulaire. L'objectif de ce doctorat était de développer des outils permettant de mieux comprendre les mécanismes ayant lieu lors de l'utilisation de nanovecteurs à base de copolymères à blocs encapsulant du phéophorbide-a et lors de l'irradiation lumineuse du photosensibilisateur. Les nanovecteurs étudiés étaient des micelles formulées à base de trois copolymères différents, le PEO-PCL, le PEO-PLA et le PEO-PS. Pour simplifier le système étudié, nous avons choisi d'utiliser des modèles de membranes pour simuler la cible biologique, des liposomes (ou vésicules lipidiques). En utilisant les propriétés de fluorescence du phéophorbide-a, nous avons pu obtenir les constantes d'affinité du photosensibilisateur pour les micelles et pour les vésicules lipidiques, puis évaluer le passage du phéophorbide-a des micelles vers les vésicules. Dans un second temps, nous nous sommes intéressés aux phénomènes en jeu lors de l'irradiation du photosensibilisateur. Nous avons pu estimer la production relative d'oxygène singulet en fonction du type de micelles utilisé. En suivant la fuite d'une sonde fluorescente contenue dans les liposomes, permettant ainsi de remonter à leur perméabilité, il a été possible mesurer les effets de la production d'oxygène singulet sur l'intégrité de la membrane des liposomes. De manière complémentaire, nous avons suivi l'oxydation des lipides constituant les liposomes durant l'irradiation du phéophorbide-a par spectrométrie de masse. Ces résultats combinés nous ont permis de voir quels étaient les paramètres influençant l'efficacité des micelles encapsulant un photosensibilisateur après irradiation et d'établir un classement de ceux ayant le plus d'effets sur l'intégrité de membranes modèles parmi ceux étudiés.

Photodynamic therapy (PDT), a therapy based on the irradiation of photosensitizing molecules to generate an oxidative stress, is already used as a treatment of some pathologies. The photosensitizers used are often highly hydrophobic molecules that aggregate in aqueous medium. Therefore, used by themselves, they require to be injected at high concentrations,…

Advisors/Committee Members: Roux, Clément (thesis director), Lonetti, Barbara (thesis director).

Subjects/Keywords: Polymère; Micelles; Liposomes; PDT; Polymer; Micelles; Liposomes; PDT

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Demazeau, M. (2019). Relations structure-effet de nanovecteurs à base de copolymères à blocs pour la thérapie photodynamique : utilisation de modèles de membranes : Structure-effect relationship of bloc copolymer based nanocarriers for photodynamic therapy : the use of model membrane. (Doctoral Dissertation). Université Toulouse III – Paul Sabatier. Retrieved from http://www.theses.fr/2019TOU30113

Chicago Manual of Style (16^th Edition):

Demazeau, Maxime. “Relations structure-effet de nanovecteurs à base de copolymères à blocs pour la thérapie photodynamique : utilisation de modèles de membranes : Structure-effect relationship of bloc copolymer based nanocarriers for photodynamic therapy : the use of model membrane.” 2019. Doctoral Dissertation, Université Toulouse III – Paul Sabatier. Accessed March 07, 2021. http://www.theses.fr/2019TOU30113.

MLA Handbook (7^th Edition):

Demazeau, Maxime. “Relations structure-effet de nanovecteurs à base de copolymères à blocs pour la thérapie photodynamique : utilisation de modèles de membranes : Structure-effect relationship of bloc copolymer based nanocarriers for photodynamic therapy : the use of model membrane.” 2019. Web. 07 Mar 2021.

Vancouver:

Demazeau M. Relations structure-effet de nanovecteurs à base de copolymères à blocs pour la thérapie photodynamique : utilisation de modèles de membranes : Structure-effect relationship of bloc copolymer based nanocarriers for photodynamic therapy : the use of model membrane. [Internet] [Doctoral dissertation]. Université Toulouse III – Paul Sabatier; 2019. [cited 2021 Mar 07]. Available from: http://www.theses.fr/2019TOU30113.

Council of Science Editors:

Demazeau M. Relations structure-effet de nanovecteurs à base de copolymères à blocs pour la thérapie photodynamique : utilisation de modèles de membranes : Structure-effect relationship of bloc copolymer based nanocarriers for photodynamic therapy : the use of model membrane. [Doctoral Dissertation]. Université Toulouse III – Paul Sabatier; 2019. Available from: http://www.theses.fr/2019TOU30113

Nelson Mandela Metropolitan University

29. Motala, Ismail Mohammed; . Formulation of an optimal non-targeted liposome preparation for fusion with tumour cell line membranes.

Degree: Faculty of Science, 2016, Nelson Mandela Metropolitan University

URL: http://hdl.handle.net/10948/12220

► The most common treatment used for cancer is chemotherapy. Chemotherapeutic agents have a greater affinity for rapidly dividing cells which is a characteristic of tumour… (more)

▼ The most common treatment used for cancer is chemotherapy. Chemotherapeutic agents have a greater affinity for rapidly dividing cells which is a characteristic of tumour cells. Although anti-cancer agents have their advantages in providing anti-cancer effects, they can be seen as highly toxic molecules posing a threat to normal healthy tissue within the human body. However, these toxic therapies need to be delivered to tumour sites without damaging healthy tissue. Liposomes can serve as a delivery system for these toxic molecules and be delivered to the tumour site via the EPR effect. Hence, liposomes that fuse with tumour cell line membranes are advantageous in delivering payloads of drugs directly into the tumour cell without damaging normal healthy tissue. The aim of the study was to formulate an optimised liposome preparation in order to enhance cellular uptake by MCF-7, Caco-2 and C3A cancer cell lines via membrane fusion. The optimal liposome formulation was aimed to be prepared utilising a statistical design approach in order to determine the ranges of the parameters that were furthermost optimal in formulating an ideal liposome preparation. The primary screening design was conducted using a 24-1 fractional factorial design that took into account the four parameters that were used to determine the optimisation of the liposomal preparation. The four variables used in the liposome preparation were the phospholipid type (PS or DOPE), the concentration of cholesteryl hemisuccinate (CHEMS) (10 – 40 %), the concentration of PEG2000-PE (0.5 – 4 %) and liposome size (100 or 200 nm). Liposomes were prepared using thin film hydration method and characterisation for size and zeta potential was carried out using photon correlation spectroscopy (PCS). Visual characterisation of liposome size was carried out using atomic force microscopy (AFM). Liposomes were exposed the cancer cell lines with visualisation and uptake being measured using fluorescent microscopy and flow cytometry, respectively. An optimal liposome preparation was prepared following the statistical design method. The optimal liposome preparation consisted of phospholipid type PS, 22.91 % of CHEMS, 4 % of PEG2000-PE and a liposome size of 200 nm. AFM analysis has shown that optimal liposome sizes ranged between 130 and 170 nm. Flow cytometry analysis indicated high level of liposome uptake with actual values falling below the predicted values set out by the statistical design. Fluorescence microscopy captured images of the fluorescent liposomes concentrated on the membrane of cells. The objective of the study was to determine from literature which variables would be desirable in preparing an optimal non-targeted liposome preparation. This was achieved by identifying four such variables and utilising them in a statistical design approach which was screened in order to determine the ideal parameters in preparing the optimised liposome batch. Therefore, from the results obtained it can be concluded that the aim of the study were met by preparing an optimal…

Subjects/Keywords: Liposomes; Cancer – Adjuvant treatment; Nanotechnology – Cancer

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Motala, I. M. (2016). Formulation of an optimal non-targeted liposome preparation for fusion with tumour cell line membranes. (Thesis). Nelson Mandela Metropolitan University. Retrieved from http://hdl.handle.net/10948/12220

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Motala, Ismail Mohammed;. “Formulation of an optimal non-targeted liposome preparation for fusion with tumour cell line membranes.” 2016. Thesis, Nelson Mandela Metropolitan University. Accessed March 07, 2021. http://hdl.handle.net/10948/12220.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Motala, Ismail Mohammed;. “Formulation of an optimal non-targeted liposome preparation for fusion with tumour cell line membranes.” 2016. Web. 07 Mar 2021.

Vancouver:

Motala IM. Formulation of an optimal non-targeted liposome preparation for fusion with tumour cell line membranes. [Internet] [Thesis]. Nelson Mandela Metropolitan University; 2016. [cited 2021 Mar 07]. Available from: http://hdl.handle.net/10948/12220.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Motala IM. Formulation of an optimal non-targeted liposome preparation for fusion with tumour cell line membranes. [Thesis]. Nelson Mandela Metropolitan University; 2016. Available from: http://hdl.handle.net/10948/12220

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

30. Καστελλοριζιός, Μιχαήλ. Σύνδεση λιποσωμικών μορφών σε επιφάνειες, που έχουν τροποποιηθεί κατάλληλα με τεχνολογία πλάσματος, με ομοιοπολικό δεσμό.

Degree: 2012, University of Patras

URL: http://hdl.handle.net/10889/5278

►

Τις τελευταίες δεκαετίες υπάρχει μια αυξανόμενη ζήτηση για βιοσυμβατά υλικά ικανά να χρησιμοποιηθούν σαν πρόσθετα στο ανθρώπινο σώμα, σαν βάσεις για ελεγχόμενη χορήγηση βιοδραστικών ενώσεων,… (more)

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Τις τελευταίες δεκαετίες υπάρχει μια αυξανόμενη ζήτηση για βιοσυμβατά υλικά ικανά να χρησιμοποιηθούν σαν πρόσθετα στο ανθρώπινο σώμα, σαν βάσεις για ελεγχόμενη χορήγηση βιοδραστικών ενώσεων, σαν εμφυτεύματα κ.α. χωρίς να προκαλούν ανοσοποιητική αντίδραση από τον αργανισμό. Μέχρι και σήμερα δεν έχει βρεθεί το υλικό που θα ξεγελάσει τους αμυντικούς μηχανισμούς του σώματος, με άλλα λόγια, να είναι αόρατο από το σώμα. Έχουν γίνει πολλές προσπάθειες και έχουν εφαρμοστεί διάφορες προσεγγίσεις. Τα τελευταία χρόνια όλο και περισσότερες ερευνητικές ομάδες επενδύουν σε υλικά τα οποία απελευθερώνουν ελεγχόμενα βιοδραστικές ουσίες που καταστέλουν την αντίδραση του οργανισμού. Μια τέτοια ουσία είναι η ηπαρίνη, ένα φυσικό αντιπηκτικό το οποίο μπορεί να χρησιμοποιηθεί για την αύξηση της αιμοσυμβατότητας αρτηριακών ενδοπροσθέσεων. Αναπτύξαμε μια μέθοδο για την ομοιοπολική, μη αναστρέψιμη πρόσδεση λιποσωμάτων σε μεταλλικές επιφάνειες, οι οποίες έχουν υποστεί επεξεργασία με τεχνολογία πλάσματος. Οι επιφάνειες φέρουν ελεύθερες καρβοξυλομάδες τις οποίες μπορούμε να εκμεταλλευτούμε για να συζεύξουμε πάνω τους λιποσώματα με αμινομάδες στην επιφάνειά τους (functionalized), μέσω δημιουργίας αμιδικού δεσμού. Οι μεταλλικές επιφάνειες που χρησιμοποιήσαμε ήταν SS-316 μεταλλικοί δίσκοι επεξεργασμένοι με τεχνολογία πλάσματος, και τις προμηθευτήκαμε από το Τμήμα Χημικών Μηχανικών του Πανεπιστημίου Πατρών και από το τμήμα Χημείας του Πανεπιστημίου του Μπάρι. Χρησιμοποιήσαμε μικρά μονοστιβαδιακά λιποσώματα (SUV) διαμέτρου της τάξης των 100 nm, με διάφορες λιπιδικές συστάσεις (PC, PC:Chol 4:1, DSPC:Chol 2:1). Χαρακτηρίσαμε τα functionalized λιποσώματά ως προς την ικανότητά τους να εγκλωβίζουν ηπαρίνη, το μέγεθος, τη διασπορά μεγέθους, το φορτίο της επιφάνειάς τους και τη σταθερότητά τους σε διάφορες συνθήκες. Τα αποτελέσματά μας δείχνουν ότι γενικά τα functionalized λιποσώματα συμπεριφέρονται σαν τυπικά λιποσώματα που φέρουν λιπίδιο με PEG ομάδα (pegylated λιποσώματα), ενώ για βέλτιστο εγκλωβισμό ηπαρίνης σημαντικό είναι το στάδιο λυοφιλοποίησης / επανασύστασης κατά την παρασκευή τους. Αποδείξαμε ότι τα functionalized λιποσώματα μπορούν να προσδεθούν σε μεταλλικές επιφάνειες (κατάλληλα επεξεργασμένες) διατηρώντας τη δομή τους και φέροντας ηπαρίνη ή άλλη υδρόφιλη ουσία στο εσωτερικό τους. Εφαρμόσαμε ένα απλό πρωτόκολλο δημιουργίας αμιδικού δεσμού, και το βελτιστοποιήσαμε ώστε να πάρουμε μέγιστη απόδοση στη σύνδεση των λιποσωμάτων στις επιφάνειες (58,4 ± 8.6 μg λιπιδίου ανά cm2 επιφάνειας).

Over the last couple of decades there has been an increasing need for more biocompatible, more “body friendly” materials that can be used in cases of subcutaneous, endoarterial or other type of implantations, as artificial body parts, drug releasing bases or biosensor implantations. The main desired quality of these materials is the lack to trigger the body’s defensive mechanisms and foreign body response reactions. Up to this date there has not been a material with the ability to camouflage itself and be invisible to the human body. Lately, scientists show…

Advisors/Committee Members: Αντιμησιάρη, Σοφία, Kastellorizios, Michael, Αντιμησιάρη, Σοφία, Κλεπετσάνης, Παύλος, Ματαράς, Δημήτριος.

Subjects/Keywords: Λιποσώματα; Ηπαρίνη; Επαναστένωση; 571.655; Liposomes; Heparin; Restenosis

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Καστελλοριζιός, �. (2012). Σύνδεση λιποσωμικών μορφών σε επιφάνειες, που έχουν τροποποιηθεί κατάλληλα με τεχνολογία πλάσματος, με ομοιοπολικό δεσμό. (Masters Thesis). University of Patras. Retrieved from http://hdl.handle.net/10889/5278

Chicago Manual of Style (16^th Edition):

Καστελλοριζιός, Μιχαήλ. “Σύνδεση λιποσωμικών μορφών σε επιφάνειες, που έχουν τροποποιηθεί κατάλληλα με τεχνολογία πλάσματος, με ομοιοπολικό δεσμό.” 2012. Masters Thesis, University of Patras. Accessed March 07, 2021. http://hdl.handle.net/10889/5278.

MLA Handbook (7^th Edition):

Καστελλοριζιός, Μιχαήλ. “Σύνδεση λιποσωμικών μορφών σε επιφάνειες, που έχουν τροποποιηθεί κατάλληλα με τεχνολογία πλάσματος, με ομοιοπολικό δεσμό.” 2012. Web. 07 Mar 2021.

Vancouver:

Καστελλοριζιός �. Σύνδεση λιποσωμικών μορφών σε επιφάνειες, που έχουν τροποποιηθεί κατάλληλα με τεχνολογία πλάσματος, με ομοιοπολικό δεσμό. [Internet] [Masters thesis]. University of Patras; 2012. [cited 2021 Mar 07]. Available from: http://hdl.handle.net/10889/5278.

Council of Science Editors:

Καστελλοριζιός �. Σύνδεση λιποσωμικών μορφών σε επιφάνειες, που έχουν τροποποιηθεί κατάλληλα με τεχνολογία πλάσματος, με ομοιοπολικό δεσμό. [Masters Thesis]. University of Patras; 2012. Available from: http://hdl.handle.net/10889/5278

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Open Access Theses and Dissertations