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Rutgers University
1.
Kandrac, Morgan, 1993-.
Factors affecting the 2,4-dinitrophenyl hydrazine reaction with lipid carbonyls.
Degree: MS, Food Science, 2018, Rutgers University
URL: https://rucore.libraries.rutgers.edu/rutgers-lib/59128/
► The basic reactions of lipid oxidation were first reported more than 50 years ago, yet measurement of lipid oxidation remains a challenge for both industry…
(more)
▼ The basic reactions of
lipid oxidation were first reported more than 50 years ago, yet measurement of
lipid oxidation remains a challenge for both industry and academia. Particularly missing are sensitive and accurate methods for quantitating and identifying secondary non-volatile
oxidation products such as monomer carbonyls, both saturated and unsaturated, and core aldehydes remaining on triacylglycerols. Methods for quantitating carbonyls by reaction with 2,4-dinitrophenylhydrazine (DNPH) have been plagued with inconsistencies and problems that have limited applications to analyses of
lipid carbonyls.
This thesis has re-evaluated the chemistry underlying reaction of 2,4-dinitrophenylhydrazine with
lipid carbonyls to develop a robust method that is chemically accurate and quantitative as well as simple enough for both research and industrial quality control analyses.DNPH reaction conditions and characteristics were tested using pure aldehydes that are typical
lipid oxidation products, including saturated, monounsaturated, and diunsaturated compounds. DNPH was dissolved in N,N-dimethylformamide (DMF), acidified with varying concentrations and types of acids to form the base reagent. This was then reacted with aldehydes diluted in acetonitrile, for a range of times, and reagent mixtures were applied to HPLC columns for separation of product hydrazones, unreacted DNPH, and unreacted aldehyde (if any); this procedure also detected any side products generated. Peaks were detected by diode array detection and quantitated by comparison peak areas to standard curves generated from each aldehyde. Conditions under which saturated and unsaturated aldehydes reacted to completion, within 20 minutes, with minimal generation of hydrazone isomers and no carbonyl condensation products were identified as pH 2.52 with a molar ratio of 2.5:1 2,4-DNPH:Carbonyl. Reactions were incomplete at higher pH and were less efficient at lower pH. Reaction slopes for the various aldehydes were within 10% variation of each other in contrast to previous observations of large differences with aldehyde structure. Reaction variability was less than 2%, and limits of detection and quantification were <50 micrograms/L. Formic acid gave results comparable to those of HCl as acidifying reagent. 3,5-Diaminobenzoic acid (DABA) was unable to provide an alternate proton source without
lowering pH. A variety of HPLC columns tested - Ultra C18 with 2.1 mm internal diameter, pentafluorophenyl, and aqueous C18 - were unable to improve resolution of critical pairs of hydrazones.
Advisors/Committee Members: Schaich, Karen M (chair), School of Graduate Studies.
Subjects/Keywords: Lipid oxidation; Lipid carbonyls
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Chicago ·
MLA ·
Vancouver ·
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APA (6th Edition):
Kandrac, Morgan, 1. (2018). Factors affecting the 2,4-dinitrophenyl hydrazine reaction with lipid carbonyls. (Masters Thesis). Rutgers University. Retrieved from https://rucore.libraries.rutgers.edu/rutgers-lib/59128/
Chicago Manual of Style (16th Edition):
Kandrac, Morgan, 1993-. “Factors affecting the 2,4-dinitrophenyl hydrazine reaction with lipid carbonyls.” 2018. Masters Thesis, Rutgers University. Accessed January 28, 2021.
https://rucore.libraries.rutgers.edu/rutgers-lib/59128/.
MLA Handbook (7th Edition):
Kandrac, Morgan, 1993-. “Factors affecting the 2,4-dinitrophenyl hydrazine reaction with lipid carbonyls.” 2018. Web. 28 Jan 2021.
Vancouver:
Kandrac, Morgan 1. Factors affecting the 2,4-dinitrophenyl hydrazine reaction with lipid carbonyls. [Internet] [Masters thesis]. Rutgers University; 2018. [cited 2021 Jan 28].
Available from: https://rucore.libraries.rutgers.edu/rutgers-lib/59128/.
Council of Science Editors:
Kandrac, Morgan 1. Factors affecting the 2,4-dinitrophenyl hydrazine reaction with lipid carbonyls. [Masters Thesis]. Rutgers University; 2018. Available from: https://rucore.libraries.rutgers.edu/rutgers-lib/59128/

University of Illinois – Urbana-Champaign
2.
Haley, Hannah M.
Modular synthesis-enabled mechanistic characterization of the potent antilipoperoxidant activity of peridinin.
Degree: PhD, Chemistry, 2018, University of Illinois – Urbana-Champaign
URL: http://hdl.handle.net/2142/101323
► The peroxidation of polyunsaturated fatty acids is a hallmark of many human disorders, yet is has often remained ambiguous whether lipid peroxidation plays a causative…
(more)
▼ The peroxidation of polyunsaturated fatty acids is a hallmark of many human disorders, yet is has often remained ambiguous whether
lipid peroxidation plays a causative and therefore addressable role in the onset or pathogenesis of a disease. Small molecule inhibitors of this oxidative process in theory possess the capacity to help clarify this ambiguity, but many of the currently available compounds have important limitations. We therefore proposed that context-specific selective pressures may have promoted the evolution of natural products with potent antilipoperoxidant activity in microorganisms that thrive in environments of extreme oxidative stress. Enabled by a modular building-block synthetic platform, the structurally atypical carotenoid peridinin was found to be a potent inhibitor of non-enzymatic bilayer
lipid peroxidation.
With the goal of maximizing the utility of peridinin as a small molecule probe of the role of
lipid peroxidation in pathogenesis, the mechanistic underpinnings of its antilipoperoxidant activity were investigated. Solid-state NMR experiments with a site-selectively 13C-labeled peridinin isotopologue revealed that the potency is linked to a high effective molarity within
lipid bilayers. In contrast to the primarily extramembranous localization of the less effective antilipoperoxidant astaxanthin, peridinin is completely embedded within and physically spans the hydrophobic core of
lipid membranes.
This antilipoperoxidant probe was leveraged in human primary endothelial cell and mouse model experiments to interrogate the contribution of membrane
oxidation towards the pathogenesis of atherosclerosis and asthma. The peridinin-mediated mitigation of bilayer
lipid peroxidation was shown to attenuate monocyte-endothelial cell adhesion, a key step in atherogenesis. Utilizing mouse models of acute asthma, we found that
lipid peroxidation is alternatively not a key driver of the asthmatic phenotype. These results suggest that peridinin may have broad potential as a chemical probe to better understand the role of bilayer
lipid peroxidation in human disease.
Advisors/Committee Members: Burke, Martin D. (advisor), Burke, Martin D. (Committee Chair), Denmark, Scott E. (committee member), Imlay, James A. (committee member), van der Donk, Wilfred A. (committee member).
Subjects/Keywords: carotenoid; lipid oxidation; atherosclerosis
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APA ·
Chicago ·
MLA ·
Vancouver ·
CSE |
Export
to Zotero / EndNote / Reference
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APA (6th Edition):
Haley, H. M. (2018). Modular synthesis-enabled mechanistic characterization of the potent antilipoperoxidant activity of peridinin. (Doctoral Dissertation). University of Illinois – Urbana-Champaign. Retrieved from http://hdl.handle.net/2142/101323
Chicago Manual of Style (16th Edition):
Haley, Hannah M. “Modular synthesis-enabled mechanistic characterization of the potent antilipoperoxidant activity of peridinin.” 2018. Doctoral Dissertation, University of Illinois – Urbana-Champaign. Accessed January 28, 2021.
http://hdl.handle.net/2142/101323.
MLA Handbook (7th Edition):
Haley, Hannah M. “Modular synthesis-enabled mechanistic characterization of the potent antilipoperoxidant activity of peridinin.” 2018. Web. 28 Jan 2021.
Vancouver:
Haley HM. Modular synthesis-enabled mechanistic characterization of the potent antilipoperoxidant activity of peridinin. [Internet] [Doctoral dissertation]. University of Illinois – Urbana-Champaign; 2018. [cited 2021 Jan 28].
Available from: http://hdl.handle.net/2142/101323.
Council of Science Editors:
Haley HM. Modular synthesis-enabled mechanistic characterization of the potent antilipoperoxidant activity of peridinin. [Doctoral Dissertation]. University of Illinois – Urbana-Champaign; 2018. Available from: http://hdl.handle.net/2142/101323

Texas A&M University
3.
Cruzen, Shannon Michelle.
Plant-Based Tannins as Antioxidants in Pre-Cooked Ground Beef Patties.
Degree: MS, Animal Science, 2011, Texas A&M University
URL: http://hdl.handle.net/1969.1/ETD-TAMU-2010-08-8236
► Meat lipid oxidation causes negative quality effects, especially in further processed products. Butylated hydroxyanisole (BHA), butylated hydroxytoluene (BHT), and rosemary extract (RM) are common antioxidants,…
(more)
▼ Meat
lipid oxidation causes negative quality effects, especially in further processed products. Butylated hydroxyanisole (BHA), butylated hydroxytoluene (BHT), and rosemary extract (RM) are common antioxidants, but plant-based tannin compounds have been shown to be effective antioxidants. The objective was to evaluate antioxidant effectiveness, pH, color, and sensory effects of several tannin sources.
In study 1, 19 percent fat ground beef was mixed with no treatment (control), BHA/BHT (0.02percent), RM (0.2percent), or 0.25percent or 0.5percent powders of Chardonnay grapeseed (CG), Norton grapeseed (NG), CitruSmart (CS), or dried cherry (CP). Raw and cooked patties were aerobically stored for 0 to 5 days at 4 degrees C. Cooked patties were analyzed using the TBARS (thiobarbituric acid reactive substances) method. Raw patties were analyzed for pH, instrumental color, subjective color, and ingredient specks. Study 2 was similar, except the treatments were 0.25percent and 0.5percent powders of CG, chestnut (CN), mimosa (M), and quebracho (Q) tannins, as well as 0.5percent NG, 0.02percent BHA/BHT, 0.2percent RM, and a control.
In study 1, only the NG and CG pre-cooked patties had similar or lower TBARS values compared to RM and BHA/BHT patties. All tannin treatments, except 0.25percent CG and 0.25percent CS, lowered pH compared to the control. Patties with NG and 0.5percent CG were darker, and tannin-treated patties were not redder than the control. Patties with CG and 0.5percent NG were less yellow. Subjectively, tannin-treated patties did not have less discoloration during storage, and the CG and NG patties had numerous visible ingredient specks.
In study 2, CN, M, Q, and 0.5percent CG treated patties had low TBARS during storage. pH was slightly lower in CG and CN patties than the control. All tannin-treated patties were darker than the control patties, except patties containing 0.25percent CG and Q, and control patties had the highest red values. Tannin-treatments patties, except CN and Q patties, were less yellow. Subjectively, only 0.25percent M patties had less discoloration than control patties, while 0.5percent CN patties were more discolored.
Norton and Chardonnay grapeseed flour and chesnut, mimosa, and quebracho powders would be recommended for pre-cooked ground beef patties based on their antioxidant effectiveness in these studies.
Advisors/Committee Members: Miller, Rhonda K. (advisor), Awika, Joseph M. (committee member), Carstens, Gordon E. (committee member), Pinchak, William E. (committee member).
Subjects/Keywords: Tannins; antioxidants; lipid oxidation
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❌
APA ·
Chicago ·
MLA ·
Vancouver ·
CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Cruzen, S. M. (2011). Plant-Based Tannins as Antioxidants in Pre-Cooked Ground Beef Patties. (Masters Thesis). Texas A&M University. Retrieved from http://hdl.handle.net/1969.1/ETD-TAMU-2010-08-8236
Chicago Manual of Style (16th Edition):
Cruzen, Shannon Michelle. “Plant-Based Tannins as Antioxidants in Pre-Cooked Ground Beef Patties.” 2011. Masters Thesis, Texas A&M University. Accessed January 28, 2021.
http://hdl.handle.net/1969.1/ETD-TAMU-2010-08-8236.
MLA Handbook (7th Edition):
Cruzen, Shannon Michelle. “Plant-Based Tannins as Antioxidants in Pre-Cooked Ground Beef Patties.” 2011. Web. 28 Jan 2021.
Vancouver:
Cruzen SM. Plant-Based Tannins as Antioxidants in Pre-Cooked Ground Beef Patties. [Internet] [Masters thesis]. Texas A&M University; 2011. [cited 2021 Jan 28].
Available from: http://hdl.handle.net/1969.1/ETD-TAMU-2010-08-8236.
Council of Science Editors:
Cruzen SM. Plant-Based Tannins as Antioxidants in Pre-Cooked Ground Beef Patties. [Masters Thesis]. Texas A&M University; 2011. Available from: http://hdl.handle.net/1969.1/ETD-TAMU-2010-08-8236

California State University – Chico
4.
Barker, Jennifer L.
Lipid oxidation in females during the postexercise recovery period: one vs. two bouts of exercise
.
Degree: 2015, California State University – Chico
URL: http://hdl.handle.net/10211.3/144682
► Research has shown that there is a difference in postexercise substrate (lipid and carbohydrate) oxidation rates and duration during one bout of exercise, most notably,…
(more)
▼ Research has shown that there is a difference in postexercise substrate (
lipid
and carbohydrate)
oxidation rates and duration during one bout of exercise, most notably,
an increase in
lipid oxidation when compared to rest. However, it is unclear what
happens to substrate
oxidation rates during postexercise recovery during same-day split
exercise sessions in females. The purpose of this study was to determine if total
postexercise
lipid oxidation in recreationally athletic females is affected by frequency of
exercise (1 vs. 2 bouts) while keeping total workload the same (60 minutes at 65%
VO2max).
Seven recreationally athletic females were randomly allocated to each
treatment and participated as their own control group. During the control trial, resting VO2, substrate
oxidation (
lipid and carbohydrate) rates, RER and VO2max were
determined using indirect calorimetry. Subjects were then randomly allocated to each
treatment, either 60 minutes of exercise at 65% VO2max on a cycle ergometer, or two 30-
minute bouts at 65% VO2max on a cycle ergometer, separated by five hours. After each
bout, metabolic parameters were collected every 15 minutes for three hours. A
significant difference in both
lipid oxidation (p < 0.0001) and carbohydrate
oxidation (p
< 0.005) was found between the 60-min trial postexercise recovery period and the sum of
the split 30-min sessions. Significant differences in carbohydrate
oxidation were found
between exercise and control, and between exercise and all postexercise timepoints (p <
0.008). Although no significant differences in
lipid or carbohydrate
oxidation were found
between the control trial and any of the postexercise timepoints of any trial, trends in
increased
lipid oxidation and decreased carbohydrate
oxidation are seen. These results
may indicate that split exercise bouts of identical workload may lead to an overall greater
postexercise
lipid oxidation when combined, compared to a single exercise session.
Advisors/Committee Members: Azevedo, John L (advisor).
Subjects/Keywords: Lipid oxidation;
Postexercise recovery;
Exercise
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❌
APA ·
Chicago ·
MLA ·
Vancouver ·
CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Barker, J. L. (2015). Lipid oxidation in females during the postexercise recovery period: one vs. two bouts of exercise
. (Thesis). California State University – Chico. Retrieved from http://hdl.handle.net/10211.3/144682
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Barker, Jennifer L. “Lipid oxidation in females during the postexercise recovery period: one vs. two bouts of exercise
.” 2015. Thesis, California State University – Chico. Accessed January 28, 2021.
http://hdl.handle.net/10211.3/144682.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Barker, Jennifer L. “Lipid oxidation in females during the postexercise recovery period: one vs. two bouts of exercise
.” 2015. Web. 28 Jan 2021.
Vancouver:
Barker JL. Lipid oxidation in females during the postexercise recovery period: one vs. two bouts of exercise
. [Internet] [Thesis]. California State University – Chico; 2015. [cited 2021 Jan 28].
Available from: http://hdl.handle.net/10211.3/144682.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Barker JL. Lipid oxidation in females during the postexercise recovery period: one vs. two bouts of exercise
. [Thesis]. California State University – Chico; 2015. Available from: http://hdl.handle.net/10211.3/144682
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Penn State University
5.
Poyton, Matthew Forrest.
Metallomembranes: Characterizing Transition Metal Ion-Lipid Complexes.
Degree: 2016, Penn State University
URL: https://submit-etda.libraries.psu.edu/catalog/28756
► Herein, transition metal-lipid complexes are characterized using model membranes. We show that Cu2+, Zn2+ and Ni2+ bind to phosphatidylserine (PS) lipids and that Cu2+ also…
(more)
▼ Herein, transition metal-
lipid complexes are characterized using model membranes. We
show that Cu2+, Zn2+ and Ni2+ bind to phosphatidylserine (PS) lipids and that Cu2+ also binds with
the
lipid phosphatidylethanolamine (PE). These transition metals can induce dramatic chemical or
physical changes to the
lipid membrane upon binding. For example,
oxidation rates in the
presence of Cu2+ and hydrogen peroxide were significantly higher for bilayers containing PE than
for pure phosphatidylcholine (PC) bilayers that do not bind Cu2+. Zn2+ was found to induce
membrane blebbing upon binding to supported bilayers containing PS lipids. A second objective
of this work was to develop new analytical tools for studying transition metal-
lipid complexes.
We propose that the family of transition metal-
lipid complexes be referred to as
metallomembranes. These results suggest that metallomembrans are physiologically relevant and
may play a role in diseases where the Cu2+ concentration in the body is abnormally high.
Advisors/Committee Members: Paul S Cremer, Dissertation Advisor/Co-Advisor, Tom Mallouk, Committee Member, Ayusman Sen, Committee Member, Moses Hung Wai Chan, Committee Member.
Subjects/Keywords: Lipid Bilayers; Microfluidics; Lipid Oxidation; Fluorescence Quenching; Supported Lipid Bilayer Electrophoresis
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APA ·
Chicago ·
MLA ·
Vancouver ·
CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Poyton, M. F. (2016). Metallomembranes: Characterizing Transition Metal Ion-Lipid Complexes. (Thesis). Penn State University. Retrieved from https://submit-etda.libraries.psu.edu/catalog/28756
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Poyton, Matthew Forrest. “Metallomembranes: Characterizing Transition Metal Ion-Lipid Complexes.” 2016. Thesis, Penn State University. Accessed January 28, 2021.
https://submit-etda.libraries.psu.edu/catalog/28756.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Poyton, Matthew Forrest. “Metallomembranes: Characterizing Transition Metal Ion-Lipid Complexes.” 2016. Web. 28 Jan 2021.
Vancouver:
Poyton MF. Metallomembranes: Characterizing Transition Metal Ion-Lipid Complexes. [Internet] [Thesis]. Penn State University; 2016. [cited 2021 Jan 28].
Available from: https://submit-etda.libraries.psu.edu/catalog/28756.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Poyton MF. Metallomembranes: Characterizing Transition Metal Ion-Lipid Complexes. [Thesis]. Penn State University; 2016. Available from: https://submit-etda.libraries.psu.edu/catalog/28756
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Georgia
6.
Akinosho, Hannah Oluwafunmike.
The role of structure-function relationships in hydroxypropyl methylcellulose on emulsion stability.
Degree: 2014, University of Georgia
URL: http://hdl.handle.net/10724/28188
► The substituents on hydroxypropyl methylcellulose (HPMC) influence emulsion stability through viscosity effects, the formation of a gel network, and adsorption to the oil droplet surface.…
(more)
▼ The substituents on hydroxypropyl methylcellulose (HPMC) influence emulsion stability through viscosity effects, the formation of a gel network, and adsorption to the oil droplet surface. Hydroxypropyl contents indicated the crystallinities
of the HPMC grades, which influenced the nature of the gels formed. Dynamic oscillatory measurements indicated that entanglement networks formed in HPMC containing higher percentages of hydroxypropyl groups (>8.8%) and lesser crystalline contents.
Conversely, weak gels formed in HPMC possessing lower hydroxypropyl contents (<8.7%) and higher crystallinities. Furthermore, FT-IR studies identified differences in methyl contents, which affect the surface activity of HPMC. The emulsion studies
suggested that higher viscosities and methyl to hydroxypropyl ratios as well as a gel network structure form more stable emulsions; however, the same properties do not directly impact chemical stability. This research aims to study the role of
structure-function relationships in polysaccharide emulsifiers that provide physical and chemical stability
Subjects/Keywords: Hydroxypropyl methylcellulose; Emulsion, Lipid oxidation; Structure; Substituents
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❌
APA ·
Chicago ·
MLA ·
Vancouver ·
CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Akinosho, H. O. (2014). The role of structure-function relationships in hydroxypropyl methylcellulose on emulsion stability. (Thesis). University of Georgia. Retrieved from http://hdl.handle.net/10724/28188
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Akinosho, Hannah Oluwafunmike. “The role of structure-function relationships in hydroxypropyl methylcellulose on emulsion stability.” 2014. Thesis, University of Georgia. Accessed January 28, 2021.
http://hdl.handle.net/10724/28188.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Akinosho, Hannah Oluwafunmike. “The role of structure-function relationships in hydroxypropyl methylcellulose on emulsion stability.” 2014. Web. 28 Jan 2021.
Vancouver:
Akinosho HO. The role of structure-function relationships in hydroxypropyl methylcellulose on emulsion stability. [Internet] [Thesis]. University of Georgia; 2014. [cited 2021 Jan 28].
Available from: http://hdl.handle.net/10724/28188.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Akinosho HO. The role of structure-function relationships in hydroxypropyl methylcellulose on emulsion stability. [Thesis]. University of Georgia; 2014. Available from: http://hdl.handle.net/10724/28188
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Illinois – Urbana-Champaign
7.
Herrick, Ryan.
Evaluating lipid oxidation and sensory characteristics in bacon from immunologically castrated barrows.
Degree: MS, 0002, 2014, University of Illinois – Urbana-Champaign
URL: http://hdl.handle.net/2142/50753
► With increased time after immunological castration, lipid content of bacon increases and unsaturated fatty acid content is reduced. Our objective was to determine the effect…
(more)
▼ With increased time after immunological castration,
lipid content of bacon increases and unsaturated fatty acid content is reduced. Our objective was to determine the effect of immunological castration (Improvest®, gonadotropin releasing factor analog-diphtheria toxoid conjugate, Zoetis) management strategies [age of slaughter, and time after second dose (ASD)], on
lipid oxidation and sensory characteristics of bacon packaged under simulated food service conditions. To mimic conditions of a normal production system, all bellies from immunological castrated (IC) barrows were pooled to compare bacon from IC barrows, regardless of management strategy, to physical castrated (PC) barrows and gilts for the same traits. Bellies (N=129) from two slaughter dates were manufactured into bacon under commercial conditions. Immunological castration management strategies included 24 wk old IC pigs 4, 6, 8, or 10 wk ASD, 26 wk old IC pigs 6 wk ASD and 28 wk old IC pigs 8 wk ASD. Gilts and PC barrows were slaughtered at 24, 26, and 28 wk of age and pooled across age. Center-cut bacon was laid-out on parchment paper, packaged in oxygen-permeable poly-vinyl lined boxes, and frozen (-33°C) for 1, 4, 8, or 12 wks to simulate food service conditions. At the end of each storage period, trained sensory panelists evaluated bacon for saltiness, oxidized odor, oxidized flavor, and off flavor on a 15-cm line scale (0 cm indicated none and 15 cm indicated extreme intensity for each characteristic). At similar time points,
lipid oxidation (TBARS) and proximate analysis were determined. Both data sets (IC management strategies and IC barrows compared with PC barrows and gilts) were analyzed using the MIXED procedure in SAS with belly as the experimental unit. Model included fixed effects of treatment, wk of storage, their interaction, and blocked by kill date. In both data sets, least square means were separated using the PDIFF option with a Tukey-Kramer adjustment. Overall, as storage time increased, saltiness decreased from 1 to 12 wks (P < 0.01), oxidized odor and oxidized flavor increased (P < 0.01), and TBARS increased by 0.23-0.30 mg MDA/kg meat from 1 to 12 wks (P < 0.01). Moisture and
lipid content did not change with storage (P ≥ 0.11). There was no interaction of treatment and wk within IC treatments or within sex classes (P ≥ 0.11). Oxidized odor and flavor were unaffected by immunological castration management strategy (P > 0.21) or sex class (P > 0.31). Among bacon from IC barrows, TBARS were increased in bacon from IC barrows slaughtered at 28 wk of age, 8 wks ASD compared with bacon from IC barrows slaughtered at 24 wk of age, both 4 and 6 wks ASD (P < 0.01), with other treatments being similar to both extremes.
Lipid content was increased and moisture reduced (P < 0.01) in bacon from IC barrows 8 and 10 wks ASD compared with IC pigs at 4 and 6 wks ASD, regardless of age at slaughter. When bacon from IC treatments were pooled, TBARS tended to be increased (P = 0.06) by 0.03 mg MDA/kg meat from IC barrows compared to gilts, with…
Advisors/Committee Members: Dilger, Anna C. (advisor).
Subjects/Keywords: Bacon; Immunological castration; Improvest; Lipid oxidation
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❌
APA ·
Chicago ·
MLA ·
Vancouver ·
CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Herrick, R. (2014). Evaluating lipid oxidation and sensory characteristics in bacon from immunologically castrated barrows. (Thesis). University of Illinois – Urbana-Champaign. Retrieved from http://hdl.handle.net/2142/50753
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Herrick, Ryan. “Evaluating lipid oxidation and sensory characteristics in bacon from immunologically castrated barrows.” 2014. Thesis, University of Illinois – Urbana-Champaign. Accessed January 28, 2021.
http://hdl.handle.net/2142/50753.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Herrick, Ryan. “Evaluating lipid oxidation and sensory characteristics in bacon from immunologically castrated barrows.” 2014. Web. 28 Jan 2021.
Vancouver:
Herrick R. Evaluating lipid oxidation and sensory characteristics in bacon from immunologically castrated barrows. [Internet] [Thesis]. University of Illinois – Urbana-Champaign; 2014. [cited 2021 Jan 28].
Available from: http://hdl.handle.net/2142/50753.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Herrick R. Evaluating lipid oxidation and sensory characteristics in bacon from immunologically castrated barrows. [Thesis]. University of Illinois – Urbana-Champaign; 2014. Available from: http://hdl.handle.net/2142/50753
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Melbourne
8.
Torkamani, Amirehsan.
Effects of ultrasonic interventions on lipid oxidation in whey.
Degree: 2013, University of Melbourne
URL: http://hdl.handle.net/11343/38259
► The main objective of this study was to investigate the effect of ultrasonication on lipid oxidation in whey and its shelf stability. Influence of frequencies…
(more)
▼ The main objective of this study was to investigate the effect of ultrasonication on lipid oxidation in whey and its shelf stability. Influence of frequencies (20-2000 kHz), specific energies (8-300 kJ/kg) and ultrasound (US) processing times (10 and 30 minutes) was studied in detail.
Trials were carried out using a single transducer configuration in a 1 litter acoustic chamber. Treatments were performed at 20, 400, 1000 and 2000 kHz for 10 and 30 minutes at 37 ºC. Depending on the power delivered to the transducers and treatment times, specific energy uptake ranged between 8-390 kJ/kg. Headspace analysis, free fatty acid (FFA) determination, polar lipid quantification and free radical formation were key analytical measurements performed to characterise whey lipid composition and assess the oxidative damage.
Generation of free radicals in purified water matrix media (Milli-Q grade) as a result of ultrasonication was highest between 400 and 1000 kHz. Oxidative volatile compounds were initially detected at low concentrations in raw liquid whey samples prior to pasteurisation/sonication treatment. Thermal processing resulted in a significant increase in the concentration of ketone and aldehyde volatiles (P<0.05). This trend was carried on irrespective of whether samples were sonicated for extended period of time or not. Regardless of the concentration of free radicals developed in the system (0.34-110 μM); ultrasound treatment time did not generate additional oxidative odorant compounds. Concentration of all oxidative volatiles except for Hexanal and 2-E-Hexanal during treatments remained considerably below threshold values for human detection (P>0.05). Thermal and ultrasonic treatments applied did not significantly (P>0.05) affect the abundance of polar lipid (Posphatidyle ethanolamine, serine, inositol, choline and sphingomyelin species).
Free fatty acids concentration in raw whey samples was initially low but increased significantly after thermal treatment and incubation for 10 and 30 minutes (P<0.05). This could be due to enzymatic hydrolysis of triglycerides and phospholipids. Sonication at various conditions did not significantly (P>0.05) affect FFA concentration in comparison to control samples.
A secondary objective of this work was to evaluate the potential of high frequency ultrasound to separate fat from whey. Fresh whey was treated at 400 and 1000 kHz at different power input and durations (50%-100%, 5-30 minutes) to evaluate US potential for fat separation and extending whey shelf life. US treatment at 1000 kHz was more efficient to enhance fat separation. The volatile compound concentration was significantly reduced after the ultrasound separation of fat.
The current study suggested that ultrasonication at selected frequencies and selected specific energies did not enhance oxidative…
Subjects/Keywords: ultrasound; phospholipid; whey; standing wave; lipid oxidation
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APA (6th Edition):
Torkamani, A. (2013). Effects of ultrasonic interventions on lipid oxidation in whey. (Masters Thesis). University of Melbourne. Retrieved from http://hdl.handle.net/11343/38259
Chicago Manual of Style (16th Edition):
Torkamani, Amirehsan. “Effects of ultrasonic interventions on lipid oxidation in whey.” 2013. Masters Thesis, University of Melbourne. Accessed January 28, 2021.
http://hdl.handle.net/11343/38259.
MLA Handbook (7th Edition):
Torkamani, Amirehsan. “Effects of ultrasonic interventions on lipid oxidation in whey.” 2013. Web. 28 Jan 2021.
Vancouver:
Torkamani A. Effects of ultrasonic interventions on lipid oxidation in whey. [Internet] [Masters thesis]. University of Melbourne; 2013. [cited 2021 Jan 28].
Available from: http://hdl.handle.net/11343/38259.
Council of Science Editors:
Torkamani A. Effects of ultrasonic interventions on lipid oxidation in whey. [Masters Thesis]. University of Melbourne; 2013. Available from: http://hdl.handle.net/11343/38259

Louisiana State University
9.
Jirawatjunya, Jinjuta.
Effects of Assorted Coating Materials and Room Temperature Storage on Internal Quality and Oxidative Stability of Shell Eggs.
Degree: MS, Life Sciences, 2013, Louisiana State University
URL: etd-05312013-113630
;
https://digitalcommons.lsu.edu/gradschool_theses/2531
► Surface coating is an effective/inexpensive method to preserve internal quality and minimize weight loss of shell eggs during storage without refrigeration. No research has been…
(more)
▼ Surface coating is an effective/inexpensive method to preserve internal quality and minimize weight loss of shell eggs during storage without refrigeration. No research has been done to evaluate effects of coating on oxidative stability of cooked eggs. In the first study, soybean oil (SO) as a coating material was evaluated for its effects on internal quality and flavor volatile compounds of eggs throughout 5-weeks of storage at 25¢XC. Haugh unit (HU) and yolk index (YI) values decreased whereas weight loss increased during storage. After 5-weeks, SO-coated eggs had consistently higher HU (67.72 vs. 48.45) and YI (0.29 vs. 0.40) than the control. SO-coating significantly minimized weigh loss (0.95%) of eggs after 5-weeks storage (vs. 6.47% for the control). The major flavor volatiles in cooked eggs were aldehydes, ketones, alcohols, and sulfides. Soybean oil coted boiled eggs yielded the highest hexanal contents. In the second study, effectiveness of surface coatings using proteins, polysaccharides, or lipids in preserving quality and lipid oxidation of shell eggs was evaluated. After 5-weeks, lipid-based coatings had consistently higher HU (69.25 vs. 41.01-57.75) and YI (0.37 vs. 0.22-0.32) than control and other treatments. Lipid-based coating significantly minimized weigh loss (0.35%) of eggs after 5-weeks storage (vs. 4.84-6.84% for control and other treatments). After 5-weeks storage, compared with the control (8.37ƒÝg/100 g), hexanal of yolk of eggs coated with SO was higher (14.09ƒÝg), but that of eggs coated with alpha and beta chitosans, WPC, and MO was lower (3.24, 3.89, 4.34, and 6.13 ƒÝg). SO-coated eggs had highest TBARs values after 5-weeks storage. Palmitic, oleic, and linoleic acids were most abundant fatty acids in the yolk. The proportion of MUFAs and saturated fatty acids increased while the proportion of PUFAs decreased after 5 weeks of storage. This study demonstrated that surface coating can extend shelf-life of eggs, but potentially affect chemical compounds related to lipid oxidation in egg yolk during 5-weeks storage at 25¢XC.
Subjects/Keywords: coatings; lipid oxidation; volatile compounds; eggs
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APA ·
Chicago ·
MLA ·
Vancouver ·
CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Jirawatjunya, J. (2013). Effects of Assorted Coating Materials and Room Temperature Storage on Internal Quality and Oxidative Stability of Shell Eggs. (Masters Thesis). Louisiana State University. Retrieved from etd-05312013-113630 ; https://digitalcommons.lsu.edu/gradschool_theses/2531
Chicago Manual of Style (16th Edition):
Jirawatjunya, Jinjuta. “Effects of Assorted Coating Materials and Room Temperature Storage on Internal Quality and Oxidative Stability of Shell Eggs.” 2013. Masters Thesis, Louisiana State University. Accessed January 28, 2021.
etd-05312013-113630 ; https://digitalcommons.lsu.edu/gradschool_theses/2531.
MLA Handbook (7th Edition):
Jirawatjunya, Jinjuta. “Effects of Assorted Coating Materials and Room Temperature Storage on Internal Quality and Oxidative Stability of Shell Eggs.” 2013. Web. 28 Jan 2021.
Vancouver:
Jirawatjunya J. Effects of Assorted Coating Materials and Room Temperature Storage on Internal Quality and Oxidative Stability of Shell Eggs. [Internet] [Masters thesis]. Louisiana State University; 2013. [cited 2021 Jan 28].
Available from: etd-05312013-113630 ; https://digitalcommons.lsu.edu/gradschool_theses/2531.
Council of Science Editors:
Jirawatjunya J. Effects of Assorted Coating Materials and Room Temperature Storage on Internal Quality and Oxidative Stability of Shell Eggs. [Masters Thesis]. Louisiana State University; 2013. Available from: etd-05312013-113630 ; https://digitalcommons.lsu.edu/gradschool_theses/2531

Louisiana State University
10.
Zhu, Yongchao.
Relationship of Total Phenolic Contents, DPPH Activities and Anti-Lipid-Oxidation Capabilities of Different Bioactive Beverages and Phenolic Antioxidants.
Degree: MS, Life Sciences, 2014, Louisiana State University
URL: etd-11052014-085359
;
https://digitalcommons.lsu.edu/gradschool_theses/624
► Polyphenol compounds are widely used as antioxidants in food processing. The conventional phenolic content, scavenging DPPH free radical assays have been widely used for antioxidant…
(more)
▼ Polyphenol compounds are widely used as antioxidants in food processing. The conventional phenolic content, scavenging DPPH free radical assays have been widely used for antioxidant evaluation. This research aimed to study the correlation between scavenging DPPH (2, 2-diphenyl-1-picrylhydrazyl) free radical activity, total phenolic content and anti-lipid-oxidation capability of natural and synthetic antioxidants and four beverages drinks. Synthetic phenol antioxidants, BHA (butylated hydroxyanisole), BHT (butylated hydroxytoluene), PG (propyl gallate), TBHQ (tert-butylhydroquinone) and naturally occurring phenolics ƒÑ-T(£\-tocopherol), CA(Chlorogenic acid), GA(Gallic acid), QCT(Quercetin), RSV(Resveratrol), and SA(Syringic acid) were examined for their total phenolic content (TPC) and scavenging DPPH free radical activity. The anti-lipid-oxidation capability of these antioxidants was determined by using a fish oil emulsion which imitated the blood serum environment. CA and GA had great phenolic content, while they were in the group of lowest anti-lipid-oxidation capability. Oppositely, BHA was in the lowest group of phenolic content but provided the greatest anti-lipid-oxidation capability. The DPPH annihilation activity of GA was equivalent to PG and QCT and among the top three performances in the assay. However, its anti-lipid-oxidation capability was four times lower than PG or QCT. In general, most of the synthetic antioxidants had poor phenolic content but demonstrated better anti-lipid-oxidation capability, while, the phenolic acids such as GA and CA, were totally reverse. Only QCT and PG exhibited great performance in all the three assays. Thus, the fish oil emulsion developed in this study could be an efficient and reliable model for the evaluation of various antioxidants which overcomes the unilateral reaction of conventional spectrophotometric assays. Tea, coffee, red wine and white wine were also investigated to assess the correlations of TPC, DPPH free radical scavenging activity and anti-lipid-oxidation capability in a fish oil emulsion model. Diversities and concentrations of the major phenolics in the four beverages were also determined. With the most abundant phenolics, red wine dominated in TPC, DPPH scavenging activity, and anti-lipid-oxidation capability. However, white wine which had better performance in the TPC and DPPH assays showed the lowest capability in preventing fish lipid oxidation. The anti-lipid-oxidation capability of tea or coffee was much higher than white wine, although the three beverages had similar TPC. Therefore, the results from TPC and DPPH assays may not always correspond to the actual anti-lipid-oxidation capabilities. As the fish oil emulsion model was designed to imitate the human serum, the obtained anti-lipid-oxidation capability could closely reflect the antioxidant activity in stabilizing lipids and reducing harmful lipid oxidation products in the serum.
Subjects/Keywords: antioxidants; total phenolic content; DPPH; lipid oxidation
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❌
APA ·
Chicago ·
MLA ·
Vancouver ·
CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Zhu, Y. (2014). Relationship of Total Phenolic Contents, DPPH Activities and Anti-Lipid-Oxidation Capabilities of Different Bioactive Beverages and Phenolic Antioxidants. (Masters Thesis). Louisiana State University. Retrieved from etd-11052014-085359 ; https://digitalcommons.lsu.edu/gradschool_theses/624
Chicago Manual of Style (16th Edition):
Zhu, Yongchao. “Relationship of Total Phenolic Contents, DPPH Activities and Anti-Lipid-Oxidation Capabilities of Different Bioactive Beverages and Phenolic Antioxidants.” 2014. Masters Thesis, Louisiana State University. Accessed January 28, 2021.
etd-11052014-085359 ; https://digitalcommons.lsu.edu/gradschool_theses/624.
MLA Handbook (7th Edition):
Zhu, Yongchao. “Relationship of Total Phenolic Contents, DPPH Activities and Anti-Lipid-Oxidation Capabilities of Different Bioactive Beverages and Phenolic Antioxidants.” 2014. Web. 28 Jan 2021.
Vancouver:
Zhu Y. Relationship of Total Phenolic Contents, DPPH Activities and Anti-Lipid-Oxidation Capabilities of Different Bioactive Beverages and Phenolic Antioxidants. [Internet] [Masters thesis]. Louisiana State University; 2014. [cited 2021 Jan 28].
Available from: etd-11052014-085359 ; https://digitalcommons.lsu.edu/gradschool_theses/624.
Council of Science Editors:
Zhu Y. Relationship of Total Phenolic Contents, DPPH Activities and Anti-Lipid-Oxidation Capabilities of Different Bioactive Beverages and Phenolic Antioxidants. [Masters Thesis]. Louisiana State University; 2014. Available from: etd-11052014-085359 ; https://digitalcommons.lsu.edu/gradschool_theses/624

University of Kentucky
11.
Yang, Jiayi.
RELATIVE REACTIVITY OF PROTEIN AND LIPID TO OXIDANTS IN DIFFERENT BI-PHASIC SYSTEMS AND ITS IMPLICATION IN SAUSAGE QUALITY.
Degree: 2016, University of Kentucky
URL: https://uknowledge.uky.edu/animalsci_etds/68
► This study investigated the progression of protein and lipid oxidation in different bi-phasic model systems: simple aqueous dispersions, liposome, and oil-in-water (O/W) emulsions. Varied concentrations…
(more)
▼ This study investigated the progression of protein and lipid oxidation in different bi-phasic model systems: simple aqueous dispersions, liposome, and oil-in-water (O/W) emulsions. Varied concentrations of isolated pork myofibrillar protein (MFP) were mixed with free fatty acids (C18:1, C18:2, C18:3), lecithin, or vegetable oil to construct different lipid-protein dispersion systems, then subjected to hydroxyl radical stress at 4 °C. Comparison of the evolution of lipid oxidation with protein modification markers in all dispersion systems showed noteworthy attenuation of tryptophan fluorescence, protein carbonyl formation, and extensive polymerization of myosin in 2 h. This process preceded lipid oxidation which exhibited notable accumulations of thiobarbituric acid-reactive substances (TBARS) only after 2 h.
The study further explored the steric role of MFP in the oxidative stability of emulsions. Oxidized MFP in the continuous phase stimulated lipid oxidation in 24 h; sharply contrasting with interface-adsorbed MFP that inhibited TBARS formation nearly 90% in 24 h. Interfacial MFP from 2 h-oxidized samples exhibited attenuated tryptophan fluorescence but more pronounced myosin polymerization than MFP in the continuous phase. Due to the site distribution, interface-adsorbed MFP in general and myosin in particular provided accentuated protection of emulsions against oxidation. Similarly, soy protein isolate (SPI) and sodium caseinate (SC) acted as antioxidant barriers in O/W emulsions.
The effect of replacing pork fat by protein-stabilized soybean oil pre-emulsion on physical characteristics and oxidative stability of fresh sausages was subsequently investigated. Substitution (60%) of SC or partially denatured SPI pre-emulsified oil for fat improved sausage water-binding capacity (P < 0.05). During storage at 4 °C, cooked sausage formulated with partially denatured SPI-emulsified oil displayed a slower lipid oxidation rate throughout 14 days compared with control sausage, and SC-emulsified oil sausage had the lowest TBARS produced in the first 5 days. There was no significant difference (P > 0.05) in texture attributes (e.g., hardness, deformability, cohesiveness, and rupture force) between different formulations.
In summary, proteins as emulsifiers at the O/W interface are kinetically preferred targets of radicals compared to unsaturated lipids and proteins in the continuous phase. Such locality effect proves to be important for the physicochemical stability of emulsion-type foods.
Subjects/Keywords: Protein oxidation; Lipid oxidation; Emulsion; Interfacial protein; Sausage; Food Chemistry
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❌
APA ·
Chicago ·
MLA ·
Vancouver ·
CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Yang, J. (2016). RELATIVE REACTIVITY OF PROTEIN AND LIPID TO OXIDANTS IN DIFFERENT BI-PHASIC SYSTEMS AND ITS IMPLICATION IN SAUSAGE QUALITY. (Doctoral Dissertation). University of Kentucky. Retrieved from https://uknowledge.uky.edu/animalsci_etds/68
Chicago Manual of Style (16th Edition):
Yang, Jiayi. “RELATIVE REACTIVITY OF PROTEIN AND LIPID TO OXIDANTS IN DIFFERENT BI-PHASIC SYSTEMS AND ITS IMPLICATION IN SAUSAGE QUALITY.” 2016. Doctoral Dissertation, University of Kentucky. Accessed January 28, 2021.
https://uknowledge.uky.edu/animalsci_etds/68.
MLA Handbook (7th Edition):
Yang, Jiayi. “RELATIVE REACTIVITY OF PROTEIN AND LIPID TO OXIDANTS IN DIFFERENT BI-PHASIC SYSTEMS AND ITS IMPLICATION IN SAUSAGE QUALITY.” 2016. Web. 28 Jan 2021.
Vancouver:
Yang J. RELATIVE REACTIVITY OF PROTEIN AND LIPID TO OXIDANTS IN DIFFERENT BI-PHASIC SYSTEMS AND ITS IMPLICATION IN SAUSAGE QUALITY. [Internet] [Doctoral dissertation]. University of Kentucky; 2016. [cited 2021 Jan 28].
Available from: https://uknowledge.uky.edu/animalsci_etds/68.
Council of Science Editors:
Yang J. RELATIVE REACTIVITY OF PROTEIN AND LIPID TO OXIDANTS IN DIFFERENT BI-PHASIC SYSTEMS AND ITS IMPLICATION IN SAUSAGE QUALITY. [Doctoral Dissertation]. University of Kentucky; 2016. Available from: https://uknowledge.uky.edu/animalsci_etds/68

Texas A&M University
12.
Cong, Xiao.
Interactions of Cu2+ with Phosphatidylserine and Their Biological Implications.
Degree: PhD, Chemistry, 2015, Texas A&M University
URL: http://hdl.handle.net/1969.1/155048
► Phosphatidylserine (PS) lipids in cell membranes play significant roles in cell apoptosis, blood clotting and neurodegenerative disorders such as Alzheimer’s disease (AD). A novel fluorescence…
(more)
▼ Phosphatidylserine (PS) lipids in cell membranes play significant roles in cell apoptosis, blood clotting and neurodegenerative disorders such as Alzheimer’s disease (AD). A novel fluorescence quenching assay based on
lipid bilayer-coated microfluidic platform was developed to investigate the interactions of PS, Cu2+ and PS-related proteins on membranes. PS in supported
lipid bilayers (SLBs) was found to bind Cu2+ and the resulting Cu(PS)2 complex could quench a broad spectrum of
lipid-bound fluorophores in a reversible and pH-dependent fashion.
This quenching assay was subsequently combined with other spectroscopy techniques to explore how Cu2+-binding affinity changes as a function of PS surface density. The apparent binding affinity of Cu2+ to PS got enhanced by about five orders of magnitude as the PS density in SLBs was increased from 1.0 to 20 mol %. Despite the effect of binding valency change from monovalent to bivalent, this binding enhancement was primarily attributed to the greatly amplified Cu2+ concentration in the double layer of the surface with a high PS density, since Cu2+-binding preserves the surface charges.
Cu2+-PS interactions have numerous biological implications under specific physiological or pathological conditions. Herein two typical examples were investigated. First one involves the interactions of β-amyloid peptides (Aβ), Cu2+ and
lipid membranes. Aβ is an important biomarker of Alzheimer’s disease (AD) while Aβ-binding of transition metal ions can promote its pathological assembly. We applied our fluorescence assay and mass spectrometry (MS) to mimic how Aβ acquires Cu2+ from cell membranes in AD brains where copper level is generally reported to be depressed. The Cu2+ detachment by Aβ was detected by monitoring the recovery of the fluorescent signal quenched by Cu(PS)2 complex. This Cu2+ transfer behavior can be confirmed by MS detection of different complexes. The results could assist clarifying the membrane-related neurotoxicity of Aβ and copper homeostasis at pathological conditions. The combination of fluorescence and MS can be further applied in investigations of other Cu2+-mediated interactions between proteins and membranes. Another biological implication is about the effect of Cu2+-PS binding on
lipid oxidations. With PS in vesicles, the
oxidation of surrounding unsaturated phosphatidylcholine (PC) lipids was inhibited, which implies that PS in the membranes might protect other lipids against the Cu2+-catalyzed
oxidation.
Advisors/Committee Members: Cremer, Paul S (advisor), Russell, David H (advisor), Reinhart, Gregory D (committee member), Hilty, Christian (committee member).
Subjects/Keywords: Lipid membrane; Transition metal ions; Ligand-receptor Interaction; Fluorescence; Alzheimer's Disease; Lipid oxidation damage
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❌
APA ·
Chicago ·
MLA ·
Vancouver ·
CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Cong, X. (2015). Interactions of Cu2+ with Phosphatidylserine and Their Biological Implications. (Doctoral Dissertation). Texas A&M University. Retrieved from http://hdl.handle.net/1969.1/155048
Chicago Manual of Style (16th Edition):
Cong, Xiao. “Interactions of Cu2+ with Phosphatidylserine and Their Biological Implications.” 2015. Doctoral Dissertation, Texas A&M University. Accessed January 28, 2021.
http://hdl.handle.net/1969.1/155048.
MLA Handbook (7th Edition):
Cong, Xiao. “Interactions of Cu2+ with Phosphatidylserine and Their Biological Implications.” 2015. Web. 28 Jan 2021.
Vancouver:
Cong X. Interactions of Cu2+ with Phosphatidylserine and Their Biological Implications. [Internet] [Doctoral dissertation]. Texas A&M University; 2015. [cited 2021 Jan 28].
Available from: http://hdl.handle.net/1969.1/155048.
Council of Science Editors:
Cong X. Interactions of Cu2+ with Phosphatidylserine and Their Biological Implications. [Doctoral Dissertation]. Texas A&M University; 2015. Available from: http://hdl.handle.net/1969.1/155048

Iowa State University
13.
Feng, Xi.
Identification and characterization of off-odor and off-taste compounds in irradiated ready-to-eat (RTE) cooked meat products.
Degree: 2016, Iowa State University
URL: https://lib.dr.iastate.edu/etd/16052
► This study was designed to elucidate the quality changes in meat products, especially ready-to-eat (RTE) meat products under irradiation. Raw turkey breast meat, cooked turkey…
(more)
▼ This study was designed to elucidate the quality changes in meat products, especially ready-to-eat (RTE) meat products under irradiation. Raw turkey breast meat, cooked turkey breast meat and cured turkey meat products were prepared and irradiated at 0, 1.5, 3.0, and 4.5 kGy using a linear accelerator, and changes in quality parameters including color, lipid and protein oxidation, off-odor volatiles, and nucleotides and nucleotides degradation products were determined. Our results indicated that irradiation resulted in different chemical reactions to quality parameters in raw, uncured and cured cooked turkey meat products, but cured cooked turkey meat products have a higher tolerance to color changes, odor and taste deterioration than raw and uncured cooked turkey meat products due to various additives in the cured meat products. With model systems, color changes, nucleotides degradation pathway and mechanisms of off-odor produced from amino acids side chains were elucidated. Meanwhile, employing raw beef round eye, taste profile deterioration and off-odor production during irradiation was further verified by electronic tongue and multivariate statistical analyses in the red meat system in addition the white meat system.
Subjects/Keywords: Electronic tongue; Irradiation; Lipid oxidation; Nucleotides; Protein oxidation; Volatile compounds; Agriculture; Animal Sciences; Food Science
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APA ·
Chicago ·
MLA ·
Vancouver ·
CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Feng, X. (2016). Identification and characterization of off-odor and off-taste compounds in irradiated ready-to-eat (RTE) cooked meat products. (Thesis). Iowa State University. Retrieved from https://lib.dr.iastate.edu/etd/16052
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Feng, Xi. “Identification and characterization of off-odor and off-taste compounds in irradiated ready-to-eat (RTE) cooked meat products.” 2016. Thesis, Iowa State University. Accessed January 28, 2021.
https://lib.dr.iastate.edu/etd/16052.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Feng, Xi. “Identification and characterization of off-odor and off-taste compounds in irradiated ready-to-eat (RTE) cooked meat products.” 2016. Web. 28 Jan 2021.
Vancouver:
Feng X. Identification and characterization of off-odor and off-taste compounds in irradiated ready-to-eat (RTE) cooked meat products. [Internet] [Thesis]. Iowa State University; 2016. [cited 2021 Jan 28].
Available from: https://lib.dr.iastate.edu/etd/16052.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Feng X. Identification and characterization of off-odor and off-taste compounds in irradiated ready-to-eat (RTE) cooked meat products. [Thesis]. Iowa State University; 2016. Available from: https://lib.dr.iastate.edu/etd/16052
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

East Carolina University
14.
Smith, Cody D.
Linking energy state to redox environment through mitochondrial redox circuits.
Degree: PhD, PHD-Physiology, 2016, East Carolina University
URL: http://hdl.handle.net/10342/6007
► Mitochondria are the primary producers of intracellular H2O2, an oxidant signaling molecule that has gained attention for its role in normal physiological processes and disease…
(more)
▼ Mitochondria are the primary producers of intracellular H2O2, an oxidant signaling molecule that has gained attention for its role in normal physiological processes and disease etiologies such as skeletal muscle insulin resistance and type 2 diabetes. Understanding the factors that govern the rate of mitochondrial H2O2 emission is critical to developing therapeutic strategies that might mitigate H2O2-induced disease progression while simultaneously enabling cytosolic redox homeostasis and proper cell function.
Lipid catabolism through the [beta]-
oxidation pathway is a known source of mitochondrial H2O2 generation and implicated in the development of skeletal muscle insulin resistance based on its association with high-fat diets. The goal of this dissertation was 2-fold: 1) To investigate how mitochondrial H2O2 emission due to [beta]-
oxidation is regulated by inherent redox circuits with the matrix antioxidant system in skeletal muscle; and 2) To identify the bioenergetic consequences and resulting redox environment associated with increased [beta]-
oxidation flux and the development of skeletal muscle insulin resistance. To accomplish these goals, wild-type and genetically altered mouse models were used for experimentation at the whole body, muscle, and mitochondrial level. Multiple redox circuits were discovered in skeletal muscle mitochondria linking [beta]-
oxidation-induced H2O2 production to both membrane potential ([Delta][Psi]m)-dependent and independent sources of NADPH, the cofactor ultimately responsible for powering antioxidant activity. Collectively, these redox circuits regulated the rate of mitochondrial H2O2 emission by ensuring sufficient NADPH production to maintain a constant 70-80% antioxidant efficiency, regardless of the [beta]-
oxidation-flux rate. Therefore, when flux through [beta]-
oxidation was increased, thus elevating the mitochondrial energy state and rate of H2O2 production, redox circuitry enabled proportionally increased NADPH generation in order to reduce the same fraction of H2O2 as with lower energy states. Thus, an increased energy state due to increased [beta]-
oxidation-flux resulted in an increased, yet proportional rate of mitochondrial H2O2 emission. In this way, redox circuits regulate the rate of mitochondrial H2O2 emission depending on changes in energy state. At high energy states due to elevated [beta]-
oxidation flux, the increased rate of mitochondrial H2O2 emission further induced a more oxidized cytosolic redox environment, consistent with the insulin resistant phenotype observed in genetic models. Hence, redox circuitry further links mitochondrial energy state with activation/deactivation of specific redox-sensitive signaling pathways leading to changes in protein and cellular function.
Advisors/Committee Members: Neufer, P. Darrell (advisor).
Subjects/Keywords: energy; lipid; β-oxidation; H2O2; NNT; NADPH; insulin; diabetes; Oxidation-Reduction; Environment; Mitochondria
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❌
APA ·
Chicago ·
MLA ·
Vancouver ·
CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Smith, C. D. (2016). Linking energy state to redox environment through mitochondrial redox circuits. (Doctoral Dissertation). East Carolina University. Retrieved from http://hdl.handle.net/10342/6007
Chicago Manual of Style (16th Edition):
Smith, Cody D. “Linking energy state to redox environment through mitochondrial redox circuits.” 2016. Doctoral Dissertation, East Carolina University. Accessed January 28, 2021.
http://hdl.handle.net/10342/6007.
MLA Handbook (7th Edition):
Smith, Cody D. “Linking energy state to redox environment through mitochondrial redox circuits.” 2016. Web. 28 Jan 2021.
Vancouver:
Smith CD. Linking energy state to redox environment through mitochondrial redox circuits. [Internet] [Doctoral dissertation]. East Carolina University; 2016. [cited 2021 Jan 28].
Available from: http://hdl.handle.net/10342/6007.
Council of Science Editors:
Smith CD. Linking energy state to redox environment through mitochondrial redox circuits. [Doctoral Dissertation]. East Carolina University; 2016. Available from: http://hdl.handle.net/10342/6007

Massey University
15.
Brown, Colin.
Advanced platform for shelf life extension in liquid foods.
Degree: PhD, Bioprocess Engineering, 2012, Massey University
URL: http://hdl.handle.net/10179/4197
► The shelf life of lipid based foods is often determined by the development of rancid flavours attributed to lipid oxidation reactions. These reactions are highly…
(more)
▼ The shelf life of lipid based foods is often determined by the development of rancid flavours attributed to lipid oxidation reactions. These reactions are highly complicated and readily change when the reaction system is altered. As a result, researchers have struggled to make significant advances in their understanding of the mechanisms and rates of lipid oxidation.
This thesis focuses on the generalised three step mechanism of lipid oxidation and develops understanding, through mathematical modelling exercises, about the factors that influence the rates of lipid oxidation. More specifically, this thesis focuses on bulk oils, bulk oils with added antioxidants, oil-in-water emulsions and the effects of oxygen supply and consumption rates in real food systems.
For this thesis, methods were developed to identify and validate findings that suggest that lipid hydroperoxides are the rate defining reactant in lipid oxidation reactions. These methods were then used to measure the solubility of oxygen in oil and to define the role oxygen plays in determining the rates of lipid oxidation in a range of systems.
The use of a newly developed batch oxidation apparatus led to the development and validation of models to predict the rates of oxygen consumption during oxidation. The model showed that the rates of oxygen consumption were half order with respect to the lipid hydroperoxide concentration. Through further validation experiments, it was shown that, during the initial stages of lipid oxidation before rancidity, each mole of lipid hydroperoxides formed required 5.04 moles of oxygen to be consumed when there was oxygen present.
The same model and methods were then used to predict the changes in rates of lipid oxidation triggered by changes in reaction temperature. From this work, it was found that the Arrhenius law was capable of predicting the rates of oxygen consumption.
The addition of butylated hydroxyanisole (BHA) to mixed fish oil samples brought with it a reduction in the rates of lipid oxidation, the magnitude of which was proportional to the concentration of BHA added. It was found that the inclusion of a modifier into the half order model was capable of predicting the rates of lipid oxidation when antioxidants were added. Methods to quantify the modifier were supplied for future use.
The dilution of bulk oils by the formation of oil-in-water emulsions was also studied. It was found that the rates of lipid oxidation were proportional to the concentration of
lipids in the emulsion. It was shown that the extent of oxidation during a batch oxidation was inversely proportional to the concentration of lipids in the emulsions as the aqueous phase acted as sump of oxygen for reaction in the oil droplets.
Through modelling and short validation exercises, it was shown that changes to the surface area to volume ratio of oil droplets in emulsions had no effect on the rates of oxygen supply/lipid oxidation and that any effects noted in literature are likely to be the result of other surface active compounds.
Finally, a…
Subjects/Keywords: Lipids;
Lipid oxidation;
Shelf life;
Bulk oils;
Oil-in-water emulsions;
Oxidation
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MLA ·
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APA (6th Edition):
Brown, C. (2012). Advanced platform for shelf life extension in liquid foods. (Doctoral Dissertation). Massey University. Retrieved from http://hdl.handle.net/10179/4197
Chicago Manual of Style (16th Edition):
Brown, Colin. “Advanced platform for shelf life extension in liquid foods.” 2012. Doctoral Dissertation, Massey University. Accessed January 28, 2021.
http://hdl.handle.net/10179/4197.
MLA Handbook (7th Edition):
Brown, Colin. “Advanced platform for shelf life extension in liquid foods.” 2012. Web. 28 Jan 2021.
Vancouver:
Brown C. Advanced platform for shelf life extension in liquid foods. [Internet] [Doctoral dissertation]. Massey University; 2012. [cited 2021 Jan 28].
Available from: http://hdl.handle.net/10179/4197.
Council of Science Editors:
Brown C. Advanced platform for shelf life extension in liquid foods. [Doctoral Dissertation]. Massey University; 2012. Available from: http://hdl.handle.net/10179/4197

Dalhousie University
16.
Srinivassane, Sadish.
DEVELOPMENT AND EVALUATION OF OMEGA-3 FATTY ACIDS ENRICHED
CHICKEN FRANKFURTERS.
Degree: MS, Nova Scotia Agricultural College, 2011, Dalhousie University
URL: http://hdl.handle.net/10222/13204
► Chemical, physical and sensory properties of omega-3 fatty acid enriched chicken frankfurters developed with flax oil and microencapsulated fish oil at 1.2%, 2.4% and 3.6%…
(more)
▼ Chemical, physical and sensory properties of omega-3
fatty acid enriched chicken frankfurters developed with flax oil
and microencapsulated fish oil at 1.2%, 2.4% and 3.6% were
evaluated. Four replicate batches of frankfurters were produced for
texture profile analysis and TBARS for assessment of
lipid
oxidation over four weeks of refrigerated storage. Gas
chromatograph analysis indicated that omega-3 fatty acid levels
increased (p<0.05) with flax and fish oils treatments resulting
in a shift in omega-6/omega-3 with no increase in
lipid oxidation
over the storage period. The two highest levels of fish oil
resulted in increased redness, hardness, gumminess and chewiness
(p<0.05) with the highest fish oil having the lowest rating for
acceptability. 1.2 and 2.4% flax oil and 1.2% fish oil samples were
softer and juicier than commercial frankfurters. Addition of oils
high in omega-3 fatty acids to chicken-based frankfurters can
result in product resistant to
oxidation and acceptable to
consumers.
Advisors/Committee Members: Dr.Fereidoon Shahidi (external-examiner), Dian Patterson (graduate-coordinator), Dr.Bruce Rathgeber (thesis-reader), Dr.Vasantha Rupasinghe (thesis-reader), Dr.Kathleen Glover (thesis-reader), Dr.Fereidoon Shahidi (thesis-reader), Bruce Rathgeber (thesis-supervisor), Received (ethics-approval), No (manuscripts), No (copyright-release).
Subjects/Keywords: Omega-3 fatty acid; chicken frankfurters; EPA; DHA; lipid
oxidation
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APA ·
Chicago ·
MLA ·
Vancouver ·
CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Srinivassane, S. (2011). DEVELOPMENT AND EVALUATION OF OMEGA-3 FATTY ACIDS ENRICHED
CHICKEN FRANKFURTERS. (Masters Thesis). Dalhousie University. Retrieved from http://hdl.handle.net/10222/13204
Chicago Manual of Style (16th Edition):
Srinivassane, Sadish. “DEVELOPMENT AND EVALUATION OF OMEGA-3 FATTY ACIDS ENRICHED
CHICKEN FRANKFURTERS.” 2011. Masters Thesis, Dalhousie University. Accessed January 28, 2021.
http://hdl.handle.net/10222/13204.
MLA Handbook (7th Edition):
Srinivassane, Sadish. “DEVELOPMENT AND EVALUATION OF OMEGA-3 FATTY ACIDS ENRICHED
CHICKEN FRANKFURTERS.” 2011. Web. 28 Jan 2021.
Vancouver:
Srinivassane S. DEVELOPMENT AND EVALUATION OF OMEGA-3 FATTY ACIDS ENRICHED
CHICKEN FRANKFURTERS. [Internet] [Masters thesis]. Dalhousie University; 2011. [cited 2021 Jan 28].
Available from: http://hdl.handle.net/10222/13204.
Council of Science Editors:
Srinivassane S. DEVELOPMENT AND EVALUATION OF OMEGA-3 FATTY ACIDS ENRICHED
CHICKEN FRANKFURTERS. [Masters Thesis]. Dalhousie University; 2011. Available from: http://hdl.handle.net/10222/13204

Dalhousie University
17.
Sullivan Ritter, Jenna.
Chemical Measures of Fish Oil Quality: Oxidation Products
and Sensory Correlation.
Degree: PhD, Department of Process Engineering and Applied
Science, 2012, Dalhousie University
URL: http://hdl.handle.net/10222/15146
► Although quality of commercial fish oil is of the upmost importance to both suppliers and consumers, it can be difficult to maintain due to rapid…
(more)
▼ Although quality of commercial fish oil is of the
upmost importance to both suppliers and consumers, it can be
difficult to maintain due to rapid
lipid oxidation attributable to
the high levels of EPA and DHA. Fish oil quality can be assessed in
a number of ways; this paper focuses on ethyl ester (EE) content
and
oxidation products. Fish oil supplements are sold as both
triacylglycerols (TAG) and EE. TAG products are more resistant to
oxidation, have better bioavailability and are generally considered
to be of higher quality. Here, a method is described to quantify EE
in fish oil using solid phase microextraction (SPME) headspace
analysis and gas chromatography-mass spectrometry (GCMS). A related
aspect of quality is state of
oxidation and although the causes of
oxidation in fish oil are well known, there is little research on
the kinetics of the
oxidation process. The work presented here
monitors hydroperoxides to model the kinetics of
oxidation in two
commercially available fish oil supplements by fitting the data to
the Arrhenius model. It was determined that the same mechanisms of
oxidation hold at temperatures ? 40 °C and thus, this temperature
was used in the final stages of this work where accelerated
stability testing of fish oil was conducted. Currently, taste
panels are the only reliable method to assess the sensory
properties of fish oil, but these are costly and subjective.
Described here is an alternative method using SPME-GCMS to monitor
volatile
oxidation products. Two different statistical methods were
used to identify oxidative volatiles that correlate with sensory
characteristics of fish oil. First, stepwise discriminant function
analysis (DFA) was used to identify volatiles that could be used to
classify oil as acceptable or unacceptable based on sensory
analysis. Principal component analysis (PCA) and linear regression
were then applied with greater success. Both techniques identified
similar oxidative volatiles as being important to sensory
properties. It is anticipated that these methods could be adopted
by fish oil manufacturers as measures of quality.
Advisors/Committee Members: Charlotte Jacobsen (external-examiner), George Kipouros (graduate-coordinator), Gianfranco Mazzanti (thesis-reader), Peter Wentzell (thesis-reader), Suzanne Budge (thesis-supervisor), Received (ethics-approval), Yes (manuscripts), Yes (copyright-release).
Subjects/Keywords: lipid oxidation; fish oil; sensory panel; SPME; GCMS
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❌
APA ·
Chicago ·
MLA ·
Vancouver ·
CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Sullivan Ritter, J. (2012). Chemical Measures of Fish Oil Quality: Oxidation Products
and Sensory Correlation. (Doctoral Dissertation). Dalhousie University. Retrieved from http://hdl.handle.net/10222/15146
Chicago Manual of Style (16th Edition):
Sullivan Ritter, Jenna. “Chemical Measures of Fish Oil Quality: Oxidation Products
and Sensory Correlation.” 2012. Doctoral Dissertation, Dalhousie University. Accessed January 28, 2021.
http://hdl.handle.net/10222/15146.
MLA Handbook (7th Edition):
Sullivan Ritter, Jenna. “Chemical Measures of Fish Oil Quality: Oxidation Products
and Sensory Correlation.” 2012. Web. 28 Jan 2021.
Vancouver:
Sullivan Ritter J. Chemical Measures of Fish Oil Quality: Oxidation Products
and Sensory Correlation. [Internet] [Doctoral dissertation]. Dalhousie University; 2012. [cited 2021 Jan 28].
Available from: http://hdl.handle.net/10222/15146.
Council of Science Editors:
Sullivan Ritter J. Chemical Measures of Fish Oil Quality: Oxidation Products
and Sensory Correlation. [Doctoral Dissertation]. Dalhousie University; 2012. Available from: http://hdl.handle.net/10222/15146
18.
Sidibe, Amadou.
Analyses protéomiques d'une communauté bactérienne du sol et
de Rhodanobacter thiooxydans se développant en présence de subérine
de pomme de terre.
Degree: M. Sc., Biologie, 2015, Université de Sherbrooke
URL: http://www.collectionscanada.gc.ca/obj/thesescanada/vol2/QSHERU/TC-QSHERU-11143_8060.pdf
;
http://savoirs.usherbrooke.ca/bitstream/11143/8060/1/Sidibe_Amadou_MSc_2015.pdf
► Résumé: La subérine, un polymère lipidique et complexe des plantes est retrouvé dans divers tissus dont le périderme de la pomme de terre. Le processus…
(more)
▼ Résumé: La subérine, un polymère lipidique et complexe
des plantes est retrouvé dans divers tissus dont le périderme de la
pomme de terre. Le processus biologique de sa dégradation reste
encore peu connu et est attribué aux champignons. Des échantillons
de sol provenant d'un champ de pommes de terre ont été inoculés
dans un milieu de culture contenant de la subérine comme source de
carbone. Une approche métaprotéomique a été utilisée pour
identifier les populations bactériennes qui se développent en
présence de la subérine sur une période d'incubation de 60 jours.
Le nombre de spectres normalisé (NSpC) des protéines
extracellulaires produites par la communauté bactérienne du sol ont
considérablement diminué du jour 5 au jour 20, puis ont augmenté
lentement, révélant une succession de bactéries, où la population
des bactéries du genre Pseudomonas à croissance rapide a diminué et
a été remplacée par d’autres espèces bactériennes qui pouvaient se
développer en présence de la subérine. La récalcitrance de la
subérine a été démontrée par l'émergence de bactéries auxotrophes
telles qu’Oscillatoria dans les derniers jours de la culture
bactérienne. Néanmoins, l'identification de deux lipases dans le
surnageant de la culture suggère qu'au moins certaines espèces
bactériennes peuvent dégrader la subérine. Une des lipases (I4WGM2)
a été associée à Rhodanobacter thiooxydans. Lorsque cultivée dans
un milieu contenant de la subérine, la souche de R. thiooxidans
LCS2 a produit trois lipases, dont I4WGM2. R. thiooxidans LCS2 a
également produit d'autres protéines liées au métabolisme des
lipides, des transporteurs de chaines d’acide gras et les enzymes
de la [béta]-oxydation. Ceci suggère que R. thiooxydans pourrait
participer à la dégradation de la subérine.
Advisors/Committee Members: Beaulieu, Carole, Toussaint, Vicky.
Subjects/Keywords: Bacterial succession; Beta-oxidation; Lipase; Lipid metabolism; Metaproteomics
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❌
APA ·
Chicago ·
MLA ·
Vancouver ·
CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Sidibe, A. (2015). Analyses protéomiques d'une communauté bactérienne du sol et
de Rhodanobacter thiooxydans se développant en présence de subérine
de pomme de terre. (Masters Thesis). Université de Sherbrooke. Retrieved from http://www.collectionscanada.gc.ca/obj/thesescanada/vol2/QSHERU/TC-QSHERU-11143_8060.pdf ; http://savoirs.usherbrooke.ca/bitstream/11143/8060/1/Sidibe_Amadou_MSc_2015.pdf
Chicago Manual of Style (16th Edition):
Sidibe, Amadou. “Analyses protéomiques d'une communauté bactérienne du sol et
de Rhodanobacter thiooxydans se développant en présence de subérine
de pomme de terre.” 2015. Masters Thesis, Université de Sherbrooke. Accessed January 28, 2021.
http://www.collectionscanada.gc.ca/obj/thesescanada/vol2/QSHERU/TC-QSHERU-11143_8060.pdf ; http://savoirs.usherbrooke.ca/bitstream/11143/8060/1/Sidibe_Amadou_MSc_2015.pdf.
MLA Handbook (7th Edition):
Sidibe, Amadou. “Analyses protéomiques d'une communauté bactérienne du sol et
de Rhodanobacter thiooxydans se développant en présence de subérine
de pomme de terre.” 2015. Web. 28 Jan 2021.
Vancouver:
Sidibe A. Analyses protéomiques d'une communauté bactérienne du sol et
de Rhodanobacter thiooxydans se développant en présence de subérine
de pomme de terre. [Internet] [Masters thesis]. Université de Sherbrooke; 2015. [cited 2021 Jan 28].
Available from: http://www.collectionscanada.gc.ca/obj/thesescanada/vol2/QSHERU/TC-QSHERU-11143_8060.pdf ; http://savoirs.usherbrooke.ca/bitstream/11143/8060/1/Sidibe_Amadou_MSc_2015.pdf.
Council of Science Editors:
Sidibe A. Analyses protéomiques d'une communauté bactérienne du sol et
de Rhodanobacter thiooxydans se développant en présence de subérine
de pomme de terre. [Masters Thesis]. Université de Sherbrooke; 2015. Available from: http://www.collectionscanada.gc.ca/obj/thesescanada/vol2/QSHERU/TC-QSHERU-11143_8060.pdf ; http://savoirs.usherbrooke.ca/bitstream/11143/8060/1/Sidibe_Amadou_MSc_2015.pdf

University of Saskatchewan
19.
Li, He 1986-.
THE LENTIL-MEAT SYSTEM: INVESTIGATING THE ANTIOXIDANT EFFECT OF LENTIL ON COLOUR AND LIPID OXIDATION OF RAW BEEF BURGERS.
Degree: 2017, University of Saskatchewan
URL: http://hdl.handle.net/10388/7807
► Discoloration and lipid oxidation are the main deteriorative causes of raw meat products. The flour of lentil, when heat treated, was found to protect fresh…
(more)
▼ Discoloration and
lipid oxidation are the main deteriorative causes of raw meat products. The flour of lentil, when heat treated, was found to protect fresh meat colour and inhibit
lipid oxidation when incorporated into raw meat products. In order to postulate a possible mechanism of this useful functionality of lentil flour, three studies were conducted. In the first study, the impact of infrared heating to 115 and 150 °C and water bath heating of 90 °C (30 min) of different seed components of two Canadian lentil cultivars were evaluated. Enzyme activities, soluble proteins and phenolics that promote and negate
oxidation reactions were assayed. The second study was designed to investigate the effects of seed coat and cotyledon with or without heat treatment in relation to enzyme and antioxidant activities in the ground meat system. It was investigated the effects on colour parameters (L*, a* and b*) and myoglobin redox states (met-, oxy- and deoxy-) of the product surface and
lipid oxidation (thiobarbituric acid reactive substances: TBARS) during the refrigerated storage of lentil-ground meat product for 7 days. In the third study, the usability of lentil as a binder was evaluated when ground beef burgers containing the same levels of lentil components were stored for 12 weeks under frozen (-20 °C) condition, in terms of the effect on colour, myoglobin redox states and
lipid oxidation.
In the first study, it was found that the lipoxygenase, peroxidase and glutathione reductase activities were mostly found in cotyledon rather than in seed coat. The seed coat exhibited higher superoxide dismutase activity than cotyledon. The heat treatments tested were able to deactivate lipoxygenase, peroxidase and glutathione reductase significantly (P<0.05), but not the superoxide dismutase (P>0.05). Heat treatments significantly (P<0.05) increased Fe2+ chelating activity for all samples. Soluble proteins in the seed coat (hull) showed higher antiradical (1,1-diphenyl-2-picryl-hydrazyl: DDPH and 2,2’-azinobis-(3-ethylbenzothiazoline-6-sulfonic acid): ABTS) and antioxidant (ferric reducing antioxidant potential: FRAP, Fe2+ chelating) activities than those obtained from the cotyledon, and this corresponded with the higher amount of protein-bound phenolic compounds. The extracts (water and 70% (v/v) ethanol) of the seed coat contained a higher (P<0.05) level of total phenolics, condensed tannins and total flavonoids than cotyledon extracts. Water and 70% (v/v) ethanol extracts from the seed coat were able to reduce approximately 10 - 20% metmyoglobin to form oxymyoglobin, but the cotyledon extract could not. The addition of metmyoglobin was found to dramatically increase the rate of linoleic acid
oxidation by 1000 fold. However, the addition of 70% (v/v) ethanol and water extracts of seed coat were able to significantly lower (P<0.05) such high
oxidation rate initiated by metmyoglobin. The water extracts of cotyledon when used at higher levels were able to lower the
oxidation rate, but were not as effective as the water extract of…
Advisors/Committee Members: Shand, Phyllis, Wanasundara, Janitha, Tanaka, Takuji, Nickerson, Michael, Bandy, Brain, Juárez, Manuel.
Subjects/Keywords: Lentil; Antioxidant; Meat colour; Myoglobin; Lipid oxidation; TBARS
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❌
APA ·
Chicago ·
MLA ·
Vancouver ·
CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Li, H. 1. (2017). THE LENTIL-MEAT SYSTEM: INVESTIGATING THE ANTIOXIDANT EFFECT OF LENTIL ON COLOUR AND LIPID OXIDATION OF RAW BEEF BURGERS. (Thesis). University of Saskatchewan. Retrieved from http://hdl.handle.net/10388/7807
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Li, He 1986-. “THE LENTIL-MEAT SYSTEM: INVESTIGATING THE ANTIOXIDANT EFFECT OF LENTIL ON COLOUR AND LIPID OXIDATION OF RAW BEEF BURGERS.” 2017. Thesis, University of Saskatchewan. Accessed January 28, 2021.
http://hdl.handle.net/10388/7807.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Li, He 1986-. “THE LENTIL-MEAT SYSTEM: INVESTIGATING THE ANTIOXIDANT EFFECT OF LENTIL ON COLOUR AND LIPID OXIDATION OF RAW BEEF BURGERS.” 2017. Web. 28 Jan 2021.
Vancouver:
Li H1. THE LENTIL-MEAT SYSTEM: INVESTIGATING THE ANTIOXIDANT EFFECT OF LENTIL ON COLOUR AND LIPID OXIDATION OF RAW BEEF BURGERS. [Internet] [Thesis]. University of Saskatchewan; 2017. [cited 2021 Jan 28].
Available from: http://hdl.handle.net/10388/7807.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Li H1. THE LENTIL-MEAT SYSTEM: INVESTIGATING THE ANTIOXIDANT EFFECT OF LENTIL ON COLOUR AND LIPID OXIDATION OF RAW BEEF BURGERS. [Thesis]. University of Saskatchewan; 2017. Available from: http://hdl.handle.net/10388/7807
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Texas A&M University
20.
Duncan, Christopher Edward.
Factors Influencing the Stability and Marketability of a Novel, Phytochemical-Rich Oil from the Açai Palm Fruit (Euterpe oleracea Mart.).
Degree: PhD, Food Science and Technology, 2012, Texas A&M University
URL: http://hdl.handle.net/1969.1/ETD-TAMU-2010-12-8789
► The açai palm fruit has recently become the focus of numerous research endeavors due to its extraordinary antioxidant content. However, little is known about the…
(more)
▼ The açai palm fruit has recently become the focus of numerous research
endeavors due to its extraordinary antioxidant content. However, little is known about
the fruit’s phytochemical rich oil, which is a by-product of the açai pulp. Therefore, the
aim of this study was to investigate the phytochemical content of açai oil and its relation
to oxidative stability.
A total of 206 mg/kg of chlorophylls, which included chlorophyll a as well as
four chlorophyll derivatives were tentatively identified by HPLC in crude açai oil
(CAO). Two predominant carotenoids (216 mg/kg β-carotene and 177 mg/kg lutein)
were also characterized in addition to α-tocopherol (645 mg/kg). Initial investigations
into oil stability focused upon the photooxidation of açai oil due to its significant
chlorophyll content and findings demonstrated that the increases of nonanal were
observed when phospholipids and polyphenolics were reduced. Subsequently,
investigations into the interactions between phospholipids and lipophilic antioxidants
and their contributions to the stability of açai oil were also assessed by isolating
phytochemicals and selectively reconstituting the oil. Findings demonstrated a potential relationship between phospholipids and lipophilic antioxidants, but this was not
conclusive.
Advances in the processing of the açai pulp by-product created from the
clarification process yielded a partially refined açai oil (RAO). The stability of both
RAO and CAO as a result of autoxidation were compared to other common food oils
(olive, canola, and soybean) and primary and secondary
oxidation data suggested CAO
was most stable. The difference in the stability of the two açai oils was also investigated
by blending these oils and assessing
oxidation. An increased stability was demonstrated
in the blended RAO, which suggested a significant antioxidant contribution from the
CAO. With such similar lipophilic compositions in CAO and RAO, it is theorized that
the driving factor behind the stability of CAO can be attributed to its water soluble
antioxidant content.
While further investigations are required to fully comprehend the interactions of
açai oil phytochemicals, these experiments provide insight into the phytochemical
content and stability of açai oil. The understanding and information obtained in these
studies is geared at increasing the marketability of açai oil as a food ingredient.
Advisors/Committee Members: Talcott, Stephen T. (advisor), Taylor, Thomas M. (committee member), Riaz, Mian N. (committee member), McDonald, Thomas J. (committee member).
Subjects/Keywords: Acai; Acai oil; lipid oxidation; crude oil; antioxidants
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❌
APA ·
Chicago ·
MLA ·
Vancouver ·
CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Duncan, C. E. (2012). Factors Influencing the Stability and Marketability of a Novel, Phytochemical-Rich Oil from the Açai Palm Fruit (Euterpe oleracea Mart.). (Doctoral Dissertation). Texas A&M University. Retrieved from http://hdl.handle.net/1969.1/ETD-TAMU-2010-12-8789
Chicago Manual of Style (16th Edition):
Duncan, Christopher Edward. “Factors Influencing the Stability and Marketability of a Novel, Phytochemical-Rich Oil from the Açai Palm Fruit (Euterpe oleracea Mart.).” 2012. Doctoral Dissertation, Texas A&M University. Accessed January 28, 2021.
http://hdl.handle.net/1969.1/ETD-TAMU-2010-12-8789.
MLA Handbook (7th Edition):
Duncan, Christopher Edward. “Factors Influencing the Stability and Marketability of a Novel, Phytochemical-Rich Oil from the Açai Palm Fruit (Euterpe oleracea Mart.).” 2012. Web. 28 Jan 2021.
Vancouver:
Duncan CE. Factors Influencing the Stability and Marketability of a Novel, Phytochemical-Rich Oil from the Açai Palm Fruit (Euterpe oleracea Mart.). [Internet] [Doctoral dissertation]. Texas A&M University; 2012. [cited 2021 Jan 28].
Available from: http://hdl.handle.net/1969.1/ETD-TAMU-2010-12-8789.
Council of Science Editors:
Duncan CE. Factors Influencing the Stability and Marketability of a Novel, Phytochemical-Rich Oil from the Açai Palm Fruit (Euterpe oleracea Mart.). [Doctoral Dissertation]. Texas A&M University; 2012. Available from: http://hdl.handle.net/1969.1/ETD-TAMU-2010-12-8789

Texas A&M University
21.
Roybal, Tabitha Lynn.
Sorghum Bran, Chestnut Wood Powder, and Chardonnay Grape Seed Flour Addition Effect on Lipid Oxidation and Color in Ground Beef Patties.
Degree: MS, Food Science and Technology, 2012, Texas A&M University
URL: http://hdl.handle.net/1969.1/ETD-TAMU-2010-12-8672
► Natural, plant-based tannin antioxidants are capable of inhibiting lipid oxidation in ground beef and may be possible alternatives to industry synthetic and natural standards of…
(more)
▼ Natural, plant-based tannin antioxidants are capable of inhibiting
lipid oxidation in ground beef and may be possible alternatives to industry synthetic and natural standards of BHA/BHT and rosemary extract, respectively.
Ground beef was purchased on three different days, each defining a batch during study 1. Treatments, added based on meat weight, included a control, 0.2 percent rosemary (RM), 0.02 percent BHA/BHT, 0.5 percent Chardonnay grape seed flour (CG), 0.1 percent and 0.25 percent chestnut wood flour (CN), and 0.25 percent and 0.5 percent of four sorghum bran varieties: black (BS), black with tannin (BTS), white (WS), and high tannin (TS). Patties, formed in duplicate, were randomly designated as cooked or raw and by 0 to 5 day storage, and were aerobically stored at 4 degrees C. Cooked patties were analyzed using the TBARS method. Raw patties were analyzed for subjective and objective color, number of ingredient specks, and pH.
In study 2, six treatments were chosen for sensory evaluation including a control, 0.2 percent RM, 0.02 percent BHA/BHT, 0.5 percent CG, 0.1 percent CN, and 0.5 percent BTS. Preparation, and raw and cooked analysis occurred similar to study 1. Additional patties were made for day 1 consumption by consumer panelists.
In study 1, all treatments except 0.25 percent WS reduced TBARS values over time compared to 0.2 percent RM. Four treatments (0.5 percent BTS, 0.5 percent CG, 0.25 percent CN, and 0.1 percent CN) showed no significant increase in TBARS values over storage. BS and BTS yielded the lowest color space values (CIE L*, a*, and b*; P<0.0001). Ingredient specks were possible color measurement influences.
In both studies antioxidant addition reduced TBARS values over time compared to the control (P<0.0001), and percent discoloration was highest in patties containing a sorghum treatment (P<0.0001).
Patties containing 0.1 percent CN were significantly favored in terms of overall like (P<0.0002) and flavor like (P<0.0001). Patties containing 0.2 percent RM were ranked lowest in overall and flavor like, and ground beef-like bite. Patties containing 0.5 perent CG were least liked according to tenderness level (P<0.005).
These results indicate that CG, CN, and certain varieties of sorghum bran can be added to pre-cooked ground beef products and provide better antioxidant protection than currently used ingredients of BHA/BHT and rosemary extract.
Advisors/Committee Members: Miller, Rhonda K. (advisor), Rooney, Lloyd (committee member), Awika, Joseph M. (committee member), Murano, Peter S. (committee member).
Subjects/Keywords: Tannins; Ground beef; Sorghum; Chestnut; Chardonnay grape; Lipid oxidation
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APA (6th Edition):
Roybal, T. L. (2012). Sorghum Bran, Chestnut Wood Powder, and Chardonnay Grape Seed Flour Addition Effect on Lipid Oxidation and Color in Ground Beef Patties. (Masters Thesis). Texas A&M University. Retrieved from http://hdl.handle.net/1969.1/ETD-TAMU-2010-12-8672
Chicago Manual of Style (16th Edition):
Roybal, Tabitha Lynn. “Sorghum Bran, Chestnut Wood Powder, and Chardonnay Grape Seed Flour Addition Effect on Lipid Oxidation and Color in Ground Beef Patties.” 2012. Masters Thesis, Texas A&M University. Accessed January 28, 2021.
http://hdl.handle.net/1969.1/ETD-TAMU-2010-12-8672.
MLA Handbook (7th Edition):
Roybal, Tabitha Lynn. “Sorghum Bran, Chestnut Wood Powder, and Chardonnay Grape Seed Flour Addition Effect on Lipid Oxidation and Color in Ground Beef Patties.” 2012. Web. 28 Jan 2021.
Vancouver:
Roybal TL. Sorghum Bran, Chestnut Wood Powder, and Chardonnay Grape Seed Flour Addition Effect on Lipid Oxidation and Color in Ground Beef Patties. [Internet] [Masters thesis]. Texas A&M University; 2012. [cited 2021 Jan 28].
Available from: http://hdl.handle.net/1969.1/ETD-TAMU-2010-12-8672.
Council of Science Editors:
Roybal TL. Sorghum Bran, Chestnut Wood Powder, and Chardonnay Grape Seed Flour Addition Effect on Lipid Oxidation and Color in Ground Beef Patties. [Masters Thesis]. Texas A&M University; 2012. Available from: http://hdl.handle.net/1969.1/ETD-TAMU-2010-12-8672

Texas A&M University
22.
Jenschke, Blaine Edward.
Chemical, color, and sensory attributes of sorghum bran-enhanced beef patties in a high oxygen environment.
Degree: MS, Animal Science, 2006, Texas A&M University
URL: http://hdl.handle.net/1969.1/3348
► Bottom rounds were shipped to the Rosenthal Meat Science and Technology Center, ground and enhanced with one of the following predetermined treatments: control; 0.4% sodium…
(more)
▼ Bottom rounds were shipped to the Rosenthal Meat Science and Technology Center, ground and enhanced with one of the following predetermined treatments: control; 0.4% sodium phosphates and 0.3% salt; 0.25% sorghum bran; 2.0% sorghum bran; 0.25% sorghum bran, 0.4% sodium phosphates and 0.3% salt; and 2.0% sorghum bran, 0.4% sodium phosphates, and 0.3% salt. The ground beef was formed into 226 g ground beef patties, packaged in an 80% O2 and 20% CO2 gaseous environment, and stored under retail display at 4 degrees for 0, 3, 6, or 9d. Measurements to determine rate and extent of
oxidation, rate of discoloration, and sensory characteristics were taken to evaluate the effectiveness of sorghum bran.
Patties containing the highest amount of sorghum bran had the lowest TBARS values over 9 days of storage, lower a* values, greater amounts of discoloration, darker lean color, and less cook loss (P<0.05) than control patties. Patties enhanced with the highest level of sorghum bran had lower beefy/brothy and bloody flavor aromatics, higher sorghum flavor, more bitter and burnt aftertaste, and more sandy/gritty textures (P<0.05) when compared to control patties. Patties containing the low amount of sorghum had lower TBARS values (P<0.05), but similar amounts of cook loss as the control patties. Patties containing a low sorghum level, 0.4% sodium phosphates (SP) and 0.3% salt (S) had lower (P<0.05) amounts of cook loss when compared to control patties. Patties containing low amounts of sorghum were similar to control patties in terms of redness while the addition of low sorghum, SP, and S decreased (P<0.05) the degree of redness. Patties containing low amounts of sorghum bran had similar amounts of discoloration compared to control (CONT) patties. Also, these had less bloody flavor aromatics (P<0.05), but were similar in sorghum flavor aromatics and bitter taste when compared to control patties.
The addition of sorghum bran at low levels can retard oxidative rancidity in ground beef patties without causing detrimental color changes and negatively affecting sensory attributes, while patties enhanced with 2% sorghum bran have extensive discoloration and undesirable sensory attributes.
Advisors/Committee Members: Miller, Rhonda K. (advisor), Savell, Jeff W. (committee member), Keeton, Jimmy T. (committee member), Rooney, Lloyd W. (committee member).
Subjects/Keywords: Sorghum Bran; Antioxidant; Lipid Oxidation
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APA ·
Chicago ·
MLA ·
Vancouver ·
CSE |
Export
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APA (6th Edition):
Jenschke, B. E. (2006). Chemical, color, and sensory attributes of sorghum bran-enhanced beef patties in a high oxygen environment. (Masters Thesis). Texas A&M University. Retrieved from http://hdl.handle.net/1969.1/3348
Chicago Manual of Style (16th Edition):
Jenschke, Blaine Edward. “Chemical, color, and sensory attributes of sorghum bran-enhanced beef patties in a high oxygen environment.” 2006. Masters Thesis, Texas A&M University. Accessed January 28, 2021.
http://hdl.handle.net/1969.1/3348.
MLA Handbook (7th Edition):
Jenschke, Blaine Edward. “Chemical, color, and sensory attributes of sorghum bran-enhanced beef patties in a high oxygen environment.” 2006. Web. 28 Jan 2021.
Vancouver:
Jenschke BE. Chemical, color, and sensory attributes of sorghum bran-enhanced beef patties in a high oxygen environment. [Internet] [Masters thesis]. Texas A&M University; 2006. [cited 2021 Jan 28].
Available from: http://hdl.handle.net/1969.1/3348.
Council of Science Editors:
Jenschke BE. Chemical, color, and sensory attributes of sorghum bran-enhanced beef patties in a high oxygen environment. [Masters Thesis]. Texas A&M University; 2006. Available from: http://hdl.handle.net/1969.1/3348

University of Saskatchewan
23.
Pathiratne, Subhani.
Effects of seed moisture and micronizing temperature on lentil flour properties and the stabilities of colour and unsaturated lipids of beef-lentil systems.
Degree: 2014, University of Saskatchewan
URL: http://hdl.handle.net/10388/ETD-2014-06-1622
► This study investigated the effect of seed moisture level of lentil and surface temperature of micronization (infrared heat treatment) on the physico-chemical and functional properties…
(more)
▼ This study investigated the effect of seed moisture level of lentil and surface temperature of micronization (infrared heat treatment) on the physico-chemical and functional properties of resulting flours and how these flours affected colour and unsaturated
lipid oxidation when incorporated into ground beef products. Flour from raw seed (non-tempered and non-micronized) was used as the control. Whole seeds of small green lentil (Lens culinaris L., var. Eston) without tempering (8% moisture) and tempered to 16% or 23% moisture was infrared heat treated (micronized) to 115, 130, 150 or 165 °C surface temperature. The decreased protein solubility (2-60%) and lipoxygenase (70-100%), peroxidase (32-100%) and trypsin inhibitory (up to 54%) activities of resulting flours indicated changes in the protein fraction due to heat-moisture treatment. Starch gelatinization was observed at the 23% moisture level and changes in pasting properties, and water and oil absorption capacities varied with treatment. The heat-moisture combinations modified properties of starch and protein to different degrees and, consequently, lentil flour functionalities. Incorporation of lentil flour as a binder in low fat (<10%) beef burgers at 6% (w/w) showed that flours from micronized lentil seeds enhanced retention of redness and suppression of
lipid oxidation as indicated by Hunter a* values and thiobarbituric acid reactive substances values, respectively, in a retail display setting. Investigation of total phenolics in aqueous salt extracts of lentil flours showed a decrease in content with increased micronization temperature. The antioxidant assays showed no changes in the ferric ion reducing power or reduction of hydroxyl radical scavenging and superoxide radical scavenging activities with heat-moisture treatment. Reduction of lipoxygenase and peroxidase activities was evident in lentil flour aqueous salt extracts, and the enzyme activities were localized to seed cotyledons. The myoglobin-liposome model study showed that a flour extract from the 16% moisture and 150 °C treatment resulted in a slower rate of oxymyoglobin
oxidation initiation than other treatments which had different levels of lipoxygenase and peroxidase activities. Unsaturated lipids accelerated oxymyoglobin degradation irrespective of the presence of lentil extract. The extended fresh red colour retention of ground beef due to addition of flours from micronized seed compared to that from non-micronized seed may be related to suppression of pro-oxidant activities and the activity of potential antioxidants. The putative antioxidative compounds in lentil that are available for meat components may include compounds other than lentil seed phenolics.
Advisors/Committee Members: Shand, Phyllis J., Wanasundara, Janitha P., Tyler, Robert T., Nickerson, Michael T., Warkentin, Tom D..
Subjects/Keywords: Lentil; Micronization; Tempering; Redness of fresh beef burgers; Lipid oxidation
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❌
APA ·
Chicago ·
MLA ·
Vancouver ·
CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Pathiratne, S. (2014). Effects of seed moisture and micronizing temperature on lentil flour properties and the stabilities of colour and unsaturated lipids of beef-lentil systems. (Thesis). University of Saskatchewan. Retrieved from http://hdl.handle.net/10388/ETD-2014-06-1622
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Pathiratne, Subhani. “Effects of seed moisture and micronizing temperature on lentil flour properties and the stabilities of colour and unsaturated lipids of beef-lentil systems.” 2014. Thesis, University of Saskatchewan. Accessed January 28, 2021.
http://hdl.handle.net/10388/ETD-2014-06-1622.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Pathiratne, Subhani. “Effects of seed moisture and micronizing temperature on lentil flour properties and the stabilities of colour and unsaturated lipids of beef-lentil systems.” 2014. Web. 28 Jan 2021.
Vancouver:
Pathiratne S. Effects of seed moisture and micronizing temperature on lentil flour properties and the stabilities of colour and unsaturated lipids of beef-lentil systems. [Internet] [Thesis]. University of Saskatchewan; 2014. [cited 2021 Jan 28].
Available from: http://hdl.handle.net/10388/ETD-2014-06-1622.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Pathiratne S. Effects of seed moisture and micronizing temperature on lentil flour properties and the stabilities of colour and unsaturated lipids of beef-lentil systems. [Thesis]. University of Saskatchewan; 2014. Available from: http://hdl.handle.net/10388/ETD-2014-06-1622
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Technical University of Lisbon
24.
Costa, Mariana Pereira dos Santos.
Efeito da inclusão de cenoura na qualidade da carne de coelho.
Degree: 2017, Technical University of Lisbon
URL: https://www.rcaap.pt/detail.jsp?id=oai:www.repository.utl.pt:10400.5/13517
► Dissertação de Mestrado em Engenharia Zootécnica/Produção Animal
O objectivo deste estudo foi avaliar o efeito da incorporação de cenoura nos regimes alimentares de coelhos em…
(more)
▼ Dissertação de Mestrado em Engenharia Zootécnica/Produção Animal
O objectivo deste estudo foi avaliar o efeito da incorporação de cenoura nos regimes alimentares de coelhos em crescimento e engorda, sobre o processo digestivo, a oxidação lipídica e as características físico-químicas da carne de coelho. Foram formulados quatro regimes alimentares com 3% de óleo de soja e quantidades crescentes de cenoura por substituição da polpa de citrinos: um regime controlo (R1), um regime com 7,5% de cenoura (R2), um regime com 15% de cenoura (R3) e um regime com 22,5% de cenoura (R4). As dietas foram distribuídas a 48 coelhos (12 x 4), alojados individualmente, dos 37 aos 71 dias de idade. Não se registaram diferenças significativas na quantidade ingerida diária nem no aumento diário de peso entre os regimes. Os coeficientes de utilização digestiva (CUD) não foram afectados pela incorporação de cenoura. O peso do fígado nos regimes com cenoura foi 18% menor que no regime controlo. O rendimento de carcaça foi significativamente inferior (P <0,05) nos regimes com cenoura em relação ao regime controlo. A oxidação lipídica da carne aumentou significativamente (P <0,0001) com o aumento da quantidade de cenoura e o tempo de armazenamento.
ABSTRACT - Effect of carrot inclusion on rabbit meat quality - The aim of this study was to assess the effect of carrot incorporation to growing and fattening rabbit diets on digestibility of diets, lipid oxidation and physico-chemical characteristics of rabbit meat. Four diets with 3% soybean oil and increasing amounts of carrot replacing the citrus pulp were formulated: a control diet (R1), a diet with 7,5% carrot (R2), a diet with 15% carrot (R3) and a diet with 22,5% carrot (R4). 48 rabbits (12 x 4) aged 37 to 71 days in separate housing were fed with these diets. There were no meaningful differences regarding daily feed intake or average daily gain. Total tract apparent digestibility weren’t significantly affected either. Liver weight in the carrot diets was 18% lower than in control diet. Carcass yield was significantly lower (P <0,05) in the carrot diets compared to control diet. Lipid oxidation of meat increased significantly (P <0,0001) with increasing amount of carrot and time of storage.
N/A
Advisors/Committee Members: Cunha, Luísa Almeida Lima Falcão e, Martins, Cátia Falcão.
Subjects/Keywords: coelhos; cenoura; oxidação lipídica; rabbit; carrot; lipid oxidation
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APA ·
Chicago ·
MLA ·
Vancouver ·
CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Costa, M. P. d. S. (2017). Efeito da inclusão de cenoura na qualidade da carne de coelho. (Thesis). Technical University of Lisbon. Retrieved from https://www.rcaap.pt/detail.jsp?id=oai:www.repository.utl.pt:10400.5/13517
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Costa, Mariana Pereira dos Santos. “Efeito da inclusão de cenoura na qualidade da carne de coelho.” 2017. Thesis, Technical University of Lisbon. Accessed January 28, 2021.
https://www.rcaap.pt/detail.jsp?id=oai:www.repository.utl.pt:10400.5/13517.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Costa, Mariana Pereira dos Santos. “Efeito da inclusão de cenoura na qualidade da carne de coelho.” 2017. Web. 28 Jan 2021.
Vancouver:
Costa MPdS. Efeito da inclusão de cenoura na qualidade da carne de coelho. [Internet] [Thesis]. Technical University of Lisbon; 2017. [cited 2021 Jan 28].
Available from: https://www.rcaap.pt/detail.jsp?id=oai:www.repository.utl.pt:10400.5/13517.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Costa MPdS. Efeito da inclusão de cenoura na qualidade da carne de coelho. [Thesis]. Technical University of Lisbon; 2017. Available from: https://www.rcaap.pt/detail.jsp?id=oai:www.repository.utl.pt:10400.5/13517
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Connecticut
25.
Tatiyaborworntham, Nantawat.
Effects of 4-Hydroxy-2-Nonenal on Mutant Sperm Whale Myoglobins.
Degree: MS, Animal Science, 2011, University of Connecticut
URL: https://opencommons.uconn.edu/gs_theses/207
Subjects/Keywords: Myoglobin; lipid oxidation; HNE
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APA ·
Chicago ·
MLA ·
Vancouver ·
CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Tatiyaborworntham, N. (2011). Effects of 4-Hydroxy-2-Nonenal on Mutant Sperm Whale Myoglobins. (Masters Thesis). University of Connecticut. Retrieved from https://opencommons.uconn.edu/gs_theses/207
Chicago Manual of Style (16th Edition):
Tatiyaborworntham, Nantawat. “Effects of 4-Hydroxy-2-Nonenal on Mutant Sperm Whale Myoglobins.” 2011. Masters Thesis, University of Connecticut. Accessed January 28, 2021.
https://opencommons.uconn.edu/gs_theses/207.
MLA Handbook (7th Edition):
Tatiyaborworntham, Nantawat. “Effects of 4-Hydroxy-2-Nonenal on Mutant Sperm Whale Myoglobins.” 2011. Web. 28 Jan 2021.
Vancouver:
Tatiyaborworntham N. Effects of 4-Hydroxy-2-Nonenal on Mutant Sperm Whale Myoglobins. [Internet] [Masters thesis]. University of Connecticut; 2011. [cited 2021 Jan 28].
Available from: https://opencommons.uconn.edu/gs_theses/207.
Council of Science Editors:
Tatiyaborworntham N. Effects of 4-Hydroxy-2-Nonenal on Mutant Sperm Whale Myoglobins. [Masters Thesis]. University of Connecticut; 2011. Available from: https://opencommons.uconn.edu/gs_theses/207

Iowa State University
26.
Gaudino, Nicole.
Development of lecithin-based oleogels and oleogel emulsions with stearic acid capable of enhancing probiotic viability and delaying oxidation.
Degree: 2018, Iowa State University
URL: https://lib.dr.iastate.edu/etd/17189
► Phospholipids (PL) have been shown to bind to probiotics and increase their viability. However, phospholipids are readily prone to oxidation, which hinders their use in…
(more)
▼ Phospholipids (PL) have been shown to bind to probiotics and increase their viability. However, phospholipids are readily prone to oxidation, which hinders their use in dairy products. The objectives of this study were to 1) produce and characterize semisolid soy lecithin (SL): stearic acid (SA) oleogels (LOG) and oleogel emulsions (LOGE) and 2) evaluate the viability of Lactobacillus acidophilus and Bifidobacterium lactis in the aqueous phase of LOGE. Oleogels were developed with 1 wt% water, and two gelator concentrations (20 wt% and 30 wt%) with SL:SA ratios (0:10, 3:7, 5:5, 7:3, 10:0). The same SL:SA proportions were used to prepare LOGE with 10 wt% and 20 wt% of water. Small (SAX) and wide (WAX) angle x-ray diffraction studies and polarized light microscopy were conducted to determine the nano- and microstructure of the samples. The hardness of the samples was analyzed by using a texture analyzer and the thermal properties with a differential scanning calorimeter. The results indicate that LOG were primarily formed through the entanglement of bundles of reverse worm-like micelles of SL. In contrast, LOGE were structured mainly through SA bilayers that interacted in a synergistic fashion with the SL reverse micelles network to stabilize the three dimensional network. The hardness of the LOG and LOGE increased with an increase in SA; however, in samples containing both SL and SA, LOGE were harder than LOG, demonstrating that the oleogelators have a synergistic effect.
Accelerated oxidative stability test of the oleogel emulsions were determined through measurement of peroxide value (PV) upon 5, 15, 30, and 45 days storage at room temperature (25°C). The physical and microstructural characteristics of the LOGE delayed the oxidation rate of the systems by preventing interaction between lecithin and other molecules prone to oxidation and radical species. Probiotics were inoculated into LOGE with 20% solids (5:5 SL: SA) and 10% water. LOGE were stored at 4°C aerobically, and plate counts were conducted for six weeks to determine the viability of the probiotics. The counts were compared to those of three controls: canola oil plus water, MRS broth, and MRS broth supplemented with SL. Counts were greater in LOGE than canola oil plus water controls.
The novel LOG and LOGE formulated with SA can be used as a more stable alternative to SL oleogels, particularly when the objective is to achieve semi-solid characteristics. With the potential of reduced phospholipid oxidation and increased probiotics survival, incorporation of LOGE into yogurt shows promise.
Subjects/Keywords: lipid oxidation; oleogel emulsions; oleogels; probiotics; Food Science
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❌
APA ·
Chicago ·
MLA ·
Vancouver ·
CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Gaudino, N. (2018). Development of lecithin-based oleogels and oleogel emulsions with stearic acid capable of enhancing probiotic viability and delaying oxidation. (Thesis). Iowa State University. Retrieved from https://lib.dr.iastate.edu/etd/17189
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Gaudino, Nicole. “Development of lecithin-based oleogels and oleogel emulsions with stearic acid capable of enhancing probiotic viability and delaying oxidation.” 2018. Thesis, Iowa State University. Accessed January 28, 2021.
https://lib.dr.iastate.edu/etd/17189.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Gaudino, Nicole. “Development of lecithin-based oleogels and oleogel emulsions with stearic acid capable of enhancing probiotic viability and delaying oxidation.” 2018. Web. 28 Jan 2021.
Vancouver:
Gaudino N. Development of lecithin-based oleogels and oleogel emulsions with stearic acid capable of enhancing probiotic viability and delaying oxidation. [Internet] [Thesis]. Iowa State University; 2018. [cited 2021 Jan 28].
Available from: https://lib.dr.iastate.edu/etd/17189.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Gaudino N. Development of lecithin-based oleogels and oleogel emulsions with stearic acid capable of enhancing probiotic viability and delaying oxidation. [Thesis]. Iowa State University; 2018. Available from: https://lib.dr.iastate.edu/etd/17189
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Rice University
27.
Rinaldi, Mauro Adriel.
Insight into peroxisome biogenesis and metabolism from a microscopy-based screen for Arabidopsis thaliana mutants with deviations in peroxisomal morphology, distribution, or import.
Degree: PhD, Natural Sciences, 2016, Rice University
URL: http://hdl.handle.net/1911/96547
► Key steps of vital metabolic pathways are housed in peroxisomes, essential organelles. Intensive research has advanced our understanding of peroxisomes, but there are still gaps…
(more)
▼ Key steps of vital metabolic pathways are housed in peroxisomes, essential organelles. Intensive research has advanced our understanding of peroxisomes, but there are still gaps in our models. For example, we do not fully understand the additional roles of β-
oxidation and peroxisomal cofactor homeostasis. Therefore, I conducted a microscopy-based screen for aberrant distribution of peroxisomally-targeted fluorescence in Arabidopsis thaliana. This screen uncovered novel alleles defective in
lipid body mobilization, fatty acid β-
oxidation, the glyoxylate cycle, peroxisome fission, auxin metabolism, pexophagy and other peroxisomal processes. Weak mutants in
lipid mobilization retained
lipid bodies even without displaying other defects, suggesting that microscopy was sensitive to small deficiencies and that fatty acid β-
oxidation happens at higher rates than those needed for normal growth. Moreover, analysis of these mutants revealed that fatty acid β-
oxidation was needed for peroxisomal matrix protein import and wild-type peroxisome morphology. Epistasis analysis suggested that CoA and NAD+ import contributes to the peroxisomal pool and to β-
oxidation. Mutants defective in PECTIN METHYLESTERASE 31 were also recovered, suggesting a role in
lipid mobilization for this cytosolic protein.
Peroxisomal processes rely on the action of peroxins (PEX proteins), factors needed to build and maintain peroxisomes. One of the most important peroxins is the ATPase PEX1, which together with the similar ATPase PEX6, recycles the peroxisomal matrix protein receptor PEX5 from the peroxisome membrane following cargo delivery. Mutations in human PEX1 can lead to lethal peroxisomal biogenesis disorders. However, very little is known about the role of PEX1 in plants. My screen yielded a PEX1 mutation that was lethal when homozygous, suggesting PEX1 is needed for peroxisomes and life. pex1 mutants accumulated reduced levels of PEX5 and PEX6, suggesting that PEX1 promotes PEX5 and PEX6 stability. PEX6 overexpression rescued a pex1 mutant. These data suggest that the heterohexamer model of PEX1-PEX6 function developed in other organisms is conserved in plants. Unlike PEX6 overexpression, PEX1 overexpression in Arabidopsis leads to growth defects, suggesting that PEX1 levels must remain within a narrow range. The screen performed for this thesis demonstrates the sustained power of forward genetic screens to uncover new factors in biological processes.
Advisors/Committee Members: Bartel, Bonnie (advisor), Bennett, George N (advisor), Raphael, Robert (committee member).
Subjects/Keywords: Peroxisome; PEX1; PXN; PME31; fatty acid β-oxidation; lipid mobilization
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APA ·
Chicago ·
MLA ·
Vancouver ·
CSE |
Export
to Zotero / EndNote / Reference
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APA (6th Edition):
Rinaldi, M. A. (2016). Insight into peroxisome biogenesis and metabolism from a microscopy-based screen for Arabidopsis thaliana mutants with deviations in peroxisomal morphology, distribution, or import. (Doctoral Dissertation). Rice University. Retrieved from http://hdl.handle.net/1911/96547
Chicago Manual of Style (16th Edition):
Rinaldi, Mauro Adriel. “Insight into peroxisome biogenesis and metabolism from a microscopy-based screen for Arabidopsis thaliana mutants with deviations in peroxisomal morphology, distribution, or import.” 2016. Doctoral Dissertation, Rice University. Accessed January 28, 2021.
http://hdl.handle.net/1911/96547.
MLA Handbook (7th Edition):
Rinaldi, Mauro Adriel. “Insight into peroxisome biogenesis and metabolism from a microscopy-based screen for Arabidopsis thaliana mutants with deviations in peroxisomal morphology, distribution, or import.” 2016. Web. 28 Jan 2021.
Vancouver:
Rinaldi MA. Insight into peroxisome biogenesis and metabolism from a microscopy-based screen for Arabidopsis thaliana mutants with deviations in peroxisomal morphology, distribution, or import. [Internet] [Doctoral dissertation]. Rice University; 2016. [cited 2021 Jan 28].
Available from: http://hdl.handle.net/1911/96547.
Council of Science Editors:
Rinaldi MA. Insight into peroxisome biogenesis and metabolism from a microscopy-based screen for Arabidopsis thaliana mutants with deviations in peroxisomal morphology, distribution, or import. [Doctoral Dissertation]. Rice University; 2016. Available from: http://hdl.handle.net/1911/96547

Université de Sherbrooke
28.
Sidibe, Amadou.
Analyses protéomiques d'une communauté bactérienne du sol et de Rhodanobacter thiooxydans se développant en présence de subérine de pomme de terre.
Degree: 2015, Université de Sherbrooke
URL: http://hdl.handle.net/11143/8060
► Abstract: Suberin is a complex lipidic plant polymer found in various tissues including potato periderm. The biological degradation process of suberin is poorly characterized and…
(more)
▼ Abstract: Suberin is a complex lipidic plant polymer found in various tissues including potato periderm. The biological degradation process of suberin is poorly characterized and is attributed to fungi. Soil samples from a potato field were used to inoculate a culture medium containing suberin as carbon source and a metaproteomics approach was used to identify bacterial populations that develop in the presence of suberin, over a 60-day incubation period. The normalized spectral counts of predicted extracellular proteins produced by the soil bacterial community drastically decreased from day 5 to day 20 and then slowly increased, revealing a succession of bacteria. The population of fast-growing pseudomonads declined and was replaced by species that could develop in the presence of suberin. The recalcitrance of suberin was demonstrated by the emergence of auxotrophic bacteria such as Oscillatoria in the last days of the assay. Nevertheless, the identification of two putative lipases in the culture supernatants suggests that at least some bacterial species could degrade suberin. One of the lipases (I4WGM2) was associated with Rhodanobacter thiooxydans. When grown in a suberin-containing medium, R. thiooxydans strain LCS2 produced three lipases, including I4WGM2. This strain also produced other proteins linked to
lipid metabolism, including fatty acid and
lipid transporters and [beta]-
oxidation enzymes, suggesting that R. thiooxydans could participate in suberin degradation.
Advisors/Committee Members: Beaulieu, Carole (advisor), Toussaint, Vicky (advisor).
Subjects/Keywords: Bacterial succession; Beta-oxidation; Lipase; Lipid metabolism; Metaproteomics
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Chicago ·
MLA ·
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APA (6th Edition):
Sidibe, A. (2015). Analyses protéomiques d'une communauté bactérienne du sol et de Rhodanobacter thiooxydans se développant en présence de subérine de pomme de terre. (Masters Thesis). Université de Sherbrooke. Retrieved from http://hdl.handle.net/11143/8060
Chicago Manual of Style (16th Edition):
Sidibe, Amadou. “Analyses protéomiques d'une communauté bactérienne du sol et de Rhodanobacter thiooxydans se développant en présence de subérine de pomme de terre.” 2015. Masters Thesis, Université de Sherbrooke. Accessed January 28, 2021.
http://hdl.handle.net/11143/8060.
MLA Handbook (7th Edition):
Sidibe, Amadou. “Analyses protéomiques d'une communauté bactérienne du sol et de Rhodanobacter thiooxydans se développant en présence de subérine de pomme de terre.” 2015. Web. 28 Jan 2021.
Vancouver:
Sidibe A. Analyses protéomiques d'une communauté bactérienne du sol et de Rhodanobacter thiooxydans se développant en présence de subérine de pomme de terre. [Internet] [Masters thesis]. Université de Sherbrooke; 2015. [cited 2021 Jan 28].
Available from: http://hdl.handle.net/11143/8060.
Council of Science Editors:
Sidibe A. Analyses protéomiques d'une communauté bactérienne du sol et de Rhodanobacter thiooxydans se développant en présence de subérine de pomme de terre. [Masters Thesis]. Université de Sherbrooke; 2015. Available from: http://hdl.handle.net/11143/8060

University of Minnesota
29.
Liu, Xiaoyu.
The effect of temperature and heating time on the formation of alpha, beta unsaturated hydroxyaldehydes in various vegetable oils and fats.
Degree: MS, Food science, 2014, University of Minnesota
URL: http://hdl.handle.net/11299/166843
► The formation of HNE, HHE, HOE and HDE was investigated in commercial corn, soybean, peanut, canola oils, lard and beef tallow which were heat treated…
(more)
▼ The formation of HNE, HHE, HOE and HDE was investigated in commercial corn, soybean, peanut, canola oils, lard and beef tallow which were heat treated at different temperatures (145, 165 and 185°C) for 1, 3 and 5 hours. The formation of these 4–hydroxyaldehydes was monitored as 2, 4–dinitrophenylhydrazone derivatives by HPLC. These oils and fats were selected based on their different fatty acid distributions. In general, the formation of these 4–hydroxyaldehydes increased with higher temperatures (145, 165 and 185°C), longer heating time (in the range of 0 to 5 hours) and higher unsaturation in the samples. It was found that the formation of HNE was dependent on temperature, heating time and the level of linoleic acid in the oils. Therefore, to minimize the formation of the toxic HNE in high linoleic acid containing oils, the lowest temperature and the shortest heating time should be used.
Subjects/Keywords: HHE; HNE; Linoleic acid; Linolenic acid; Lipid oxidation; Food science
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APA ·
Chicago ·
MLA ·
Vancouver ·
CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Liu, X. (2014). The effect of temperature and heating time on the formation of alpha, beta unsaturated hydroxyaldehydes in various vegetable oils and fats. (Masters Thesis). University of Minnesota. Retrieved from http://hdl.handle.net/11299/166843
Chicago Manual of Style (16th Edition):
Liu, Xiaoyu. “The effect of temperature and heating time on the formation of alpha, beta unsaturated hydroxyaldehydes in various vegetable oils and fats.” 2014. Masters Thesis, University of Minnesota. Accessed January 28, 2021.
http://hdl.handle.net/11299/166843.
MLA Handbook (7th Edition):
Liu, Xiaoyu. “The effect of temperature and heating time on the formation of alpha, beta unsaturated hydroxyaldehydes in various vegetable oils and fats.” 2014. Web. 28 Jan 2021.
Vancouver:
Liu X. The effect of temperature and heating time on the formation of alpha, beta unsaturated hydroxyaldehydes in various vegetable oils and fats. [Internet] [Masters thesis]. University of Minnesota; 2014. [cited 2021 Jan 28].
Available from: http://hdl.handle.net/11299/166843.
Council of Science Editors:
Liu X. The effect of temperature and heating time on the formation of alpha, beta unsaturated hydroxyaldehydes in various vegetable oils and fats. [Masters Thesis]. University of Minnesota; 2014. Available from: http://hdl.handle.net/11299/166843

University of Minnesota
30.
Guo, Mufan.
Storage stability of a commercial spray dried hen egg yolk powder.
Degree: PhD, Food Science, 2016, University of Minnesota
URL: http://hdl.handle.net/11299/180209
► Dehydration is a good process approach for food preservation. However, dried food products may still suffer from deterioration if store in an abused environment such…
(more)
▼ Dehydration is a good process approach for food preservation. However, dried food products may still suffer from deterioration if store in an abused environment such as high humidity (water activity (aw) > 0.6) and/or high temperature (> 45°C). These storage conditions can induce undesirable chemical reactions (disulfide bond interactions, Maillard reaction and/or lipid oxidation), resulting in a significant decrease in food quality. In this study, the storage stability of a commercial spray-dried egg yolk powder was evaluated. The dried egg yolk powder (DEY) was stored at three temperatures (room temperature, 35°C, and 45°C) and at six aw (0.05, 0.12, 0.37, 0.44, 0.54, 0.66) for at least two months, and several physicochemical changes and extent of protein aggregation were measured. The overall color change of DEY was that it became slightly darker (decrease of L* value), more red (increase of a* value), and less yellow (decrease of b* value) with increased storage time. The reaction kinetics of the L* value of DEY was also calculated using a first-order hyperbolic model. Its Q10 (rate increase with temperature increase at 10°C) was 2.9, which was more indicative of lipid oxidation, and the Ea (activation energy) was around 83 kJ/mole. The color change was mostly due to the browning pigments that were produced from the Maillard reaction and lipid oxidation. The glucose content went to zero after one-week during storage at 45°C at an aw of 0.66, confirming the occurrence of the Maillard reaction. The peroxide value of DEY storage at 45°C at aw of 0.66 was significantly increased compared to the control (vacuum packaged at -20°C), proving the occurrence of lipid oxidation. In addition, the Maillard reaction products and lipid oxidation products were both detected using the front face fluorescence spectrometer. After storage at an aw of 0.66 at 45°C for 8 weeks, protein solubility of DEY in TBS-SDS buffer [Tris-buffered saline (TBS: 20 mM Tris and 500 mM sodium chloride, pH 7.5) containing 1% sodium dodecyl sulfate (SDS, g/ml)] decreased to ~ 78% compared with that of the original DEY. Formations of buffer-soluble and –insoluble protein aggregates were discovered using SDS-PAGE. The protein aggregates were mainly formed through unfolded intermediates and unfolded states as well as direct chemical linkages. The proteins in DEY were all denatured after storage at an aw of 0.66 at 45°C for 8 weeks, resulting in numerous unfolded intermediates and states that could interact with each other to form aggregates. The spray drying process during the manufacturing of DEY also caused denaturation of protein, which explained the detection of buffer-insoluble protein aggregates in the original sample. Increases of disulfide bond links and protein-lipid interaction during storage were also found using techniques such as Raman spectrometry, fourier transform infrared spectroscopy, and front-face fluorescence spectrometry, indicating that some of the protein aggregates were induced by chemical reactions. The high molecular weight…
Subjects/Keywords: egg yolk; lipid oxidation; Maillard reaction; protein; storage; water activity
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❌
APA ·
Chicago ·
MLA ·
Vancouver ·
CSE |
Export
to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Guo, M. (2016). Storage stability of a commercial spray dried hen egg yolk powder. (Doctoral Dissertation). University of Minnesota. Retrieved from http://hdl.handle.net/11299/180209
Chicago Manual of Style (16th Edition):
Guo, Mufan. “Storage stability of a commercial spray dried hen egg yolk powder.” 2016. Doctoral Dissertation, University of Minnesota. Accessed January 28, 2021.
http://hdl.handle.net/11299/180209.
MLA Handbook (7th Edition):
Guo, Mufan. “Storage stability of a commercial spray dried hen egg yolk powder.” 2016. Web. 28 Jan 2021.
Vancouver:
Guo M. Storage stability of a commercial spray dried hen egg yolk powder. [Internet] [Doctoral dissertation]. University of Minnesota; 2016. [cited 2021 Jan 28].
Available from: http://hdl.handle.net/11299/180209.
Council of Science Editors:
Guo M. Storage stability of a commercial spray dried hen egg yolk powder. [Doctoral Dissertation]. University of Minnesota; 2016. Available from: http://hdl.handle.net/11299/180209
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