Advanced search options

Advanced Search Options 🞨

Browse by author name (“Author name starts with…”).

Find ETDs with:

in
/  
in
/  
in
/  
in

Written in Published in Earliest date Latest date

Sorted by

Results per page:

Sorted by: relevance · author · university · dateNew search

You searched for subject:(lacritin). Showing records 1 – 2 of 2 total matches.

Search Limiters

Last 2 Years | English Only

No search limiters apply to these results.

▼ Search Limiters


Virginia Tech

1. Disney, Julia L. Tear lacritin concentrations in canine keratoconjunctivitis sicca.

Degree: MS, Veterinary Medicine, 2017, Virginia Tech

Background: Keratoconjunctivitis sicca (KCS) is a chronic ocular disease of both dogs and humans that can result in ocular discomfort, corneal opacification, and vision loss. Lacritin, a protein found in the tears of many species, has been shown to play a role in lacrimation and corneal health. Because of its role as a potential lacrimostimulant, assessment of endogenous lacritin levels could reveal a correlation between lacritin and tear production in the dog. Objectives: To determine if tear lacritin concentrations are decreased in canine eyes affected by KCS. Animals: 58 client-owned dogs (tear samples from 55 eyes with normal tear production and 55 eyes diagnosed with KCS). Methods: All eyes underwent an ophthalmic exam, including Schirmer Tear Testing (STT), anterior segment assessment, and tear sample collection. Tear samples were evaluated for their total protein concentrations via BCA assay and lacritin concentrations via ELISA. Results: Total protein of canine tears is increased in KCS-affected eyes as compared to normal eyes. Tear lacritin as a component of total tear protein is significantly decreased in tears from KCS-affected eyes. When measured as a concentration (mass per volume of aqueous tears), lacritin is not significantly different between KCS-affected eyes and normal eyes, nor were they strongly correlated to STT values. Conclusions and Clinical Importance: Total tear protein levels were significantly increased in canine KCS. When quantified as a proportion of total tear sample protein, tear lacritin levels are decreased in KCS-affected eyes. Relative to tear volume, tear lacritin levels are not significantly different between KCS-affected eyes and normal eyes. Assessment of lacritin supplementation in canine KCS is warranted to evaluate potential effects on lacrimation and ocular surface health. Advisors/Committee Members: Herring, Ian P. (committeechair), Pickett, James P. (committee member), McKown, Robert L. (committee member).

Subjects/Keywords: canine keratoconjunctivitis sicca; lacritin

Record DetailsSimilar RecordsGoogle PlusoneFacebookTwitterCiteULikeMendeleyreddit

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6th Edition):

Disney, J. L. (2017). Tear lacritin concentrations in canine keratoconjunctivitis sicca. (Masters Thesis). Virginia Tech. Retrieved from http://hdl.handle.net/10919/86440

Chicago Manual of Style (16th Edition):

Disney, Julia L. “Tear lacritin concentrations in canine keratoconjunctivitis sicca.” 2017. Masters Thesis, Virginia Tech. Accessed December 12, 2019. http://hdl.handle.net/10919/86440.

MLA Handbook (7th Edition):

Disney, Julia L. “Tear lacritin concentrations in canine keratoconjunctivitis sicca.” 2017. Web. 12 Dec 2019.

Vancouver:

Disney JL. Tear lacritin concentrations in canine keratoconjunctivitis sicca. [Internet] [Masters thesis]. Virginia Tech; 2017. [cited 2019 Dec 12]. Available from: http://hdl.handle.net/10919/86440.

Council of Science Editors:

Disney JL. Tear lacritin concentrations in canine keratoconjunctivitis sicca. [Masters Thesis]. Virginia Tech; 2017. Available from: http://hdl.handle.net/10919/86440


University of Southern California

2. Ma, Tao. Expression and purification of different elastin like polypeptides (ELPs) constructs for therapeutic applications.

Degree: MS, Pharmaceutical Sciences, 2015, University of Southern California

There are many rationales for modifying recombinant proteins by using polymers, which include: i) altering permeability/diffusion via controlling their hydrodynamic radius; ii) modulating mediators of controlled release; and iii) improving their avidity to molecular/cellular targets. Many of these strategies require covalent bioconjugation, which are inefficient and may compromise protein activity. An emerging solution to this challenge is to develop gene products that act like polymers. One such polymer is known as the Elastin Like Polypeptides (ELPs). ELPs are ‘protein polymers’ composed of repetitive amino acid sequence derived from the human extracellular matrix protein called tropoelastin. In addition to their high molecular weight, which exceeds 80 kDa, ELPs undergo thermo‐responsive phase separation. ELPs are highly soluble in aqueous solutions below their transition temperature (Tt) and rapidly self‐assemble into viscous coacervate particles above Tt. Tt can be modulated by changing the molecular weight and hydrophobicity of the ELP. In this thesis, ELPs are developed as a carrier for lacritin, which is a novel tear glycoprotein that promotes tear secretion, maintains ocular surface integrity and reduces formation of lymphocytic foci in lacrimal gland. Free lacritin increases the basal tear secretion in rabbits; however, it must be administered frequently. The rationale for exploring fusions of lacritin and ELP is that they may prolong the retention on the anterior segment of the eye. In order to explore this strategy, my thesis has focused on scaling‐up the production of high purity, low endotoxin level material for subsequent evaluation treating in models of aqueous‐deficient dry eye diseases. E. coli was used for expression of the lacritin‐ELP fusion construct and size exclusion chromatography (SEC) was used to separate LV96 from cleavage product. Endotoxin removal was explored using polymyxin chromatography. This thesis represents the first report of scaling‐up production of low endotoxin level lacritin ELP fusions. Advisors/Committee Members: Hamm-Alvarez, Sarah F. (Committee Chair), Mackay, John Andrew (Committee Member), Shen, Wei-Chiang (Committee Member).

Subjects/Keywords: elastin like polypeptides; ELPs; lacritin; protein expression; scaling-up of protein purification; endotoxin removal

Record DetailsSimilar RecordsGoogle PlusoneFacebookTwitterCiteULikeMendeleyreddit

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6th Edition):

Ma, T. (2015). Expression and purification of different elastin like polypeptides (ELPs) constructs for therapeutic applications. (Masters Thesis). University of Southern California. Retrieved from http://digitallibrary.usc.edu/cdm/compoundobject/collection/p15799coll3/id/592829/rec/2685

Chicago Manual of Style (16th Edition):

Ma, Tao. “Expression and purification of different elastin like polypeptides (ELPs) constructs for therapeutic applications.” 2015. Masters Thesis, University of Southern California. Accessed December 12, 2019. http://digitallibrary.usc.edu/cdm/compoundobject/collection/p15799coll3/id/592829/rec/2685.

MLA Handbook (7th Edition):

Ma, Tao. “Expression and purification of different elastin like polypeptides (ELPs) constructs for therapeutic applications.” 2015. Web. 12 Dec 2019.

Vancouver:

Ma T. Expression and purification of different elastin like polypeptides (ELPs) constructs for therapeutic applications. [Internet] [Masters thesis]. University of Southern California; 2015. [cited 2019 Dec 12]. Available from: http://digitallibrary.usc.edu/cdm/compoundobject/collection/p15799coll3/id/592829/rec/2685.

Council of Science Editors:

Ma T. Expression and purification of different elastin like polypeptides (ELPs) constructs for therapeutic applications. [Masters Thesis]. University of Southern California; 2015. Available from: http://digitallibrary.usc.edu/cdm/compoundobject/collection/p15799coll3/id/592829/rec/2685

.