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University of Alberta
1.
Sun, Xuan.
Arsenic Speciation in Poultry Kidney.
Degree: MS, Department of Chemistry, 2014, University of Alberta
URL: https://era.library.ualberta.ca/files/cf7623c68k 
► An organoarsenic compound, 3-nitro-4-hydroxyphenyl arsenic acid (also known as Roxarsone), has been used as a feed additive. Roxarsone was approved by the Food and Drug…
(more)
▼ An organoarsenic compound, 3-nitro-4-hydroxyphenyl
arsenic acid (also known as Roxarsone), has been used as a feed
additive. Roxarsone was approved by the Food and Drug
Administration (FDA) to control diseases in poultry, and to improve
weight gain, feed efficiency, and meat pigmentation. Contrary to
the previous belief that most of the Roxarsone is excreted
unchanged in the manure, researchers from the FDA recently reported
increases in inorganic arsenic concentration in the liver of a
small number of chickens fed with Roxarsone. This thesis focuses on
a much larger scale feeding study, involving a subset of 142
chickens from a total of 1600 chickens over a 35-day period. Within
this subset, 71 chickens were fed a Roxarsone-supplemented diet,
and the other 71 chickens were fed a control diet not supplemented
with Roxarsone. The objectives of this research are to develop a
method for arsenic speciation analysis and to quantify arsenic
species in chicken kidney. Kidney samples were treated with pepsin,
and the extracts were analyzed for arsenic species using high
performance liquid chromatography (HPLC) separation with
simultaneous detection by inductively coupled plasma mass
spectrometry (ICP-MS) and electrospray ionization tandem mass
spectrometry (ESI MS/MS). The temporal profile of each arsenic
species was acquired and the analyses show the presence of eleven
arsenic compounds in the extracts of the chicken kidney samples.
HPLC-ICP-MS allowed for the quantification of the arsenic species,
and ESI MS/MS provided complementary information for the
identification of the arsenic species. Results from the analyses of
both the control and the Roxarsone-fed chickens are important to
our understanding of arsenic metabolism, distribution, and
retention in chicken.
Subjects/Keywords: arsenic; kidney
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APA (6th Edition):
Sun, X. (2014). Arsenic Speciation in Poultry Kidney. (Masters Thesis). University of Alberta. Retrieved from https://era.library.ualberta.ca/files/cf7623c68k
Chicago Manual of Style (16th Edition):
Sun, Xuan. “Arsenic Speciation in Poultry Kidney.” 2014. Masters Thesis, University of Alberta. Accessed February 20, 2020.
https://era.library.ualberta.ca/files/cf7623c68k.
MLA Handbook (7th Edition):
Sun, Xuan. “Arsenic Speciation in Poultry Kidney.” 2014. Web. 20 Feb 2020.
Vancouver:
Sun X. Arsenic Speciation in Poultry Kidney. [Internet] [Masters thesis]. University of Alberta; 2014. [cited 2020 Feb 20].
Available from: https://era.library.ualberta.ca/files/cf7623c68k.
Council of Science Editors:
Sun X. Arsenic Speciation in Poultry Kidney. [Masters Thesis]. University of Alberta; 2014. Available from: https://era.library.ualberta.ca/files/cf7623c68k
McGill University
2.
Musey, Paul Isaac.
Metabolism of free and conjugated estrogens by renal preparations in vitro.
Degree: MS, Department of Biochemistry, 1969, McGill University
URL: http://digitool.library.mcgill.ca/thesisfile147758.pdf
► Note:
Renal tissue homogenates of different species: rat, hen, rabbit and dog were used to study the metabolism of 3H-Estrone (El), Estradiol 17S (Ez); Estrone…
(more)
▼ Note:
Renal tissue homogenates of different species: rat, hen, rabbit and dog were used to study the metabolism of 3H-Estrone (El), Estradiol 17S (Ez); Estrone Sulfate (EIS) and Estradiol 17S glucuronide (EZG) separately. The metabolites were separated by various extraction procedures and the conjugates investigated by chromatography on DEAE-sephadex. The main steroid found following incubation of El or Ez in all species was El. This agrees with most in vitro studies except where gonadal tissue is concerned. More polar metabolites were virtually absent and there was no evidence of sulfurylation having occurred. Some glucuronide formation was apparent. Incubation of EIS showed a wide range of sulfatase activities, extremely high in the rat and very low in the dog. The hen, followed by the rabbit showed intermediate values. Kidney preparations from rabbit and dog appeared capable of metabolizing EIS without prior hydrolysis. Little evidence was obtained for the presence of significant S-glucuronidase activity in these tissues. On occasion it appeared that EzG could be converted to conjugated metabolites probably the N-acetyl glucosamine derivative.
Advisors/Committee Members: Hobkirk, R. (Supervisor).
Subjects/Keywords: Kidney
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University of Illinois – Chicago
3.
Yoo, Jongwon.
Predicting One-year Kidney Graft Failure and Death with Cardiovascular and Immunological Factors.
Degree: 2016, University of Illinois – Chicago
URL: http://hdl.handle.net/10027/20887
► Cardiovascular and immunological factors increase the levels of uncertainty and risk associated with transplantation. Kidney transplant programs are reluctant to risk performing transplant surgery on…
(more)
▼ Cardiovascular and immunological factors increase the levels of uncertainty and risk associated with transplantation.
Kidney transplant programs are reluctant to risk performing transplant surgery on high risk candidates because of poor outcomes. Patients with high risk have limited access to
kidney transplantation in spite of possible survival benefits of
kidney transplantation. We developed four predictive models according to donor types (living donor- and deceased donor-) and transplant outcomes (graft failure and patient death) using national transplant registry data (Scientific Registry Transplant Recipients, n = 218,657) which have different probabilities of one-year
kidney graft failure and death compared to the currently used models. We showed that by including two more risk factors in the analyses that current models underestimate predicted risks. The two factors were cardiovascular comorbidities and immunological barriers. The predictive models showed risk of high risk candidates were underestimated by current predictive models. If transplant community used our models, they would find that predicted risk of failure is higher and more generous. Then, more high risk patients could have access to
kidney transplantation without the transplant programs’ jeopardizing theirs status as high quality programs. The predictive models were shown to be valid and reliable.These models will help (1) quantify risks of transplant outcomes in high-risk candidates, (2) screen the most appropriate candidates and eventually, (3) improve accessibility of
kidney transplantation and (4) better utilize the most limited and scarce resources, donated kidneys.
Advisors/Committee Members: Ryan, Catherine (advisor), Matthews, Alicia (committee member), Murks, Catherine (committee member), Puzantian, Houry (committee member), Park, Chang Gi (committee member), Quinn, Lauretta (committee member), Collins, Eileen (committee member).
Subjects/Keywords: Kidney; Transplantation
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APA (6th Edition):
Yoo, J. (2016). Predicting One-year Kidney Graft Failure and Death with Cardiovascular and Immunological Factors. (Thesis). University of Illinois – Chicago. Retrieved from http://hdl.handle.net/10027/20887
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Yoo, Jongwon. “Predicting One-year Kidney Graft Failure and Death with Cardiovascular and Immunological Factors.” 2016. Thesis, University of Illinois – Chicago. Accessed February 20, 2020.
http://hdl.handle.net/10027/20887.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Yoo, Jongwon. “Predicting One-year Kidney Graft Failure and Death with Cardiovascular and Immunological Factors.” 2016. Web. 20 Feb 2020.
Vancouver:
Yoo J. Predicting One-year Kidney Graft Failure and Death with Cardiovascular and Immunological Factors. [Internet] [Thesis]. University of Illinois – Chicago; 2016. [cited 2020 Feb 20].
Available from: http://hdl.handle.net/10027/20887.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Yoo J. Predicting One-year Kidney Graft Failure and Death with Cardiovascular and Immunological Factors. [Thesis]. University of Illinois – Chicago; 2016. Available from: http://hdl.handle.net/10027/20887
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
McMaster University
4.
KITALA, PATRICIA.
Characterization of the Role of Shroom3 in Nephron Formation.
Degree: MSMS, 2019, McMaster University
URL: http://hdl.handle.net/11375/24826
► Proper development of the nephron, the functional unit of the kidney, is essential for kidney function. The nephron develops from a pool of cap mesenchymal…
(more)
▼ Proper development of the nephron, the functional unit of the kidney, is essential for
kidney function. The nephron develops from a pool of cap mesenchymal cells, as defined
by a cluster of cells adjacent to the ureteric bud tips of branching ureteric epithelium,
giving rise to two subset populations: the self renewing cells and the nephron progenitors.
These nephron progenitors undergo mesenchymal-epithelial transition (MET) to develop
into polarized renal vesicles (RV), and eventually fuse with the epithelial tubule to
develop into a mature nephron. Although these processes are essential for the formation
of functional kidneys, little is known about the molecular mechanisms that regulate them.
In this study, we characterize several steps during cap mesenchyme and renal vesicle
formation using our Shroom3 knockout mouse kidney as our model. Previous researchers
have associated Shroom3 with chronic kidney disease. Detecting and analyzing the
genetic components of CKD is needed to improve our understanding of its pathogenesis.
Shroom3 encodes an actin-binding protein that regulates cell shape changes through
induction of apical constriction. However, there is a lack of evidence about Shroom3’s
expression pattern and functional role upstream of developed nephrons. Here, I defined
the spatial and temporal expression of Shroom3 within the cap mesenchyme region. I
investigated the nephron progenitors between Shroom3 wildtypes and mutants. Lastly, I
analyzed the renal vesicle polarity in mutants, by analyzing apical membrane markers on
RVs to characterize any abnormalities in their orientation and establishment of polarity.
Thesis
Master of Science in Medical Sciences (MSMS)
Advisors/Committee Members: BRIDGEWATER, DARREN.
Subjects/Keywords: Shroom3; Kidney
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APA (6th Edition):
KITALA, P. (2019). Characterization of the Role of Shroom3 in Nephron Formation. (Masters Thesis). McMaster University. Retrieved from http://hdl.handle.net/11375/24826
Chicago Manual of Style (16th Edition):
KITALA, PATRICIA. “Characterization of the Role of Shroom3 in Nephron Formation.” 2019. Masters Thesis, McMaster University. Accessed February 20, 2020.
http://hdl.handle.net/11375/24826.
MLA Handbook (7th Edition):
KITALA, PATRICIA. “Characterization of the Role of Shroom3 in Nephron Formation.” 2019. Web. 20 Feb 2020.
Vancouver:
KITALA P. Characterization of the Role of Shroom3 in Nephron Formation. [Internet] [Masters thesis]. McMaster University; 2019. [cited 2020 Feb 20].
Available from: http://hdl.handle.net/11375/24826.
Council of Science Editors:
KITALA P. Characterization of the Role of Shroom3 in Nephron Formation. [Masters Thesis]. McMaster University; 2019. Available from: http://hdl.handle.net/11375/24826
University of Aberdeen
5.
Clark, Laura Elizabeth.
The epidemiology of chronic kidney disease in Grampian.
Degree: 2009, University of Aberdeen
URL: http://digitool.abdn.ac.uk:80/webclient/DeliveryManager?pid=33407
;
http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.509144
► Methods: All patients (5606) with at least one serum creatinine ≥130μmol/L in females and ≥150μmol/L (Index creatinine) in males during a 6 month period in…
(more)
▼ Methods: All patients (5606) with at least one serum creatinine ≥130μmol/L in females and ≥150μmol/L (Index creatinine) in males during a 6 month period in 2003 were grouped according to whether they had Acute Kidney Injury (AKI), Acute on chronic renal failure (ACRF) and chronic kidney disease (CKD). 1903 patients could not be classified. After using all available creatinine data and identifying markers of kidney damage a further group of patients with CKD were identified. Case records were examined for the presence of co-morbidity, date of death, cause of death and whether they were known to a renal physician. Results: 1225 patients were identified as having CKD out of the 1903 “Unclassified” cohort (65%). The majority of CKD patients were elderly females with Stage 3 CKD. Hypertension and ischaemic heart disease were the two most common co-morbid conditions. Only 12% of CKD patients were referred to a nephrologists. 43% of CKD patients were dead at follow-up mostly from cardiovascular causes (31st December 2005). The presence of proteinuria was independently associated with death. The age adjusted standardised prevalence of CKD, excluding those on RRT, was 20929 per million adult population. 3.6% went on to start RRT by the end of follow-up. Conclusions: CKD is predominantly a condition of elderly females, associated with considerable morbidity and mortality. However the majority of patients die from cardiovascular disease before progressing to ESRD. Therefore these patients may be appropriately managed in primary care without the need for specialist renal input allowing targeting of the specialist renal resources to the fewer patients who require them.
Subjects/Keywords: 616.6; Kidney Diseases : Kidney Failure, Chronic
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APA (6th Edition):
Clark, L. E. (2009). The epidemiology of chronic kidney disease in Grampian. (Doctoral Dissertation). University of Aberdeen. Retrieved from http://digitool.abdn.ac.uk:80/webclient/DeliveryManager?pid=33407 ; http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.509144
Chicago Manual of Style (16th Edition):
Clark, Laura Elizabeth. “The epidemiology of chronic kidney disease in Grampian.” 2009. Doctoral Dissertation, University of Aberdeen. Accessed February 20, 2020.
http://digitool.abdn.ac.uk:80/webclient/DeliveryManager?pid=33407 ; http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.509144.
MLA Handbook (7th Edition):
Clark, Laura Elizabeth. “The epidemiology of chronic kidney disease in Grampian.” 2009. Web. 20 Feb 2020.
Vancouver:
Clark LE. The epidemiology of chronic kidney disease in Grampian. [Internet] [Doctoral dissertation]. University of Aberdeen; 2009. [cited 2020 Feb 20].
Available from: http://digitool.abdn.ac.uk:80/webclient/DeliveryManager?pid=33407 ; http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.509144.
Council of Science Editors:
Clark LE. The epidemiology of chronic kidney disease in Grampian. [Doctoral Dissertation]. University of Aberdeen; 2009. Available from: http://digitool.abdn.ac.uk:80/webclient/DeliveryManager?pid=33407 ; http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.509144
University of Minnesota
6.
Leither, Maxwell.
Outpatient Acute Kidney Injury Increases Risk of Mortality and Chronic Kidney Disease.
Degree: MS, Clinical Research, 2017, University of Minnesota
URL: http://hdl.handle.net/11299/198958
► Introduction: Limited data exist regarding outcomes of patients with outpatient acute kidney injury (AKI). To determine whether outpatient AKI is associated with increased mortality and…
(more)
▼ Introduction: Limited data exist regarding outcomes of patients with outpatient acute kidney injury (AKI). To determine whether outpatient AKI is associated with increased mortality and chronic kidney disease (CKD), we conducted a retrospective cohort study utilizing an electronic health record in Minnesota. Methods: All adult patients receiving primary care through Fairview Health Services were included. All outpatient Cr values during an 18 month exposure period were used to define five comparator groups as follows: No outpatient AKI (reference group), outpatient AKI with recovery, outpatient AKI without recovery, outpatient AKI without repeat Cr, or no Cr. A Cox proportional hazard model was utilized to assess whether outpatient AKI was associated with an increase in mortality, CKD stage 4 and secondary outcomes including hospitalization and recurrent AKI. Results: The cohort consisted of 384,869 patients and 51% had at least one Cr measured during the exposure period. Outpatient AKI occurred in 1.4% of patients during the 18 month exposure period and 37.8% recovered while 26.5% had no repeat Cr. Mortality was 3.2% over an average follow-up of 5.3 years. Outpatient AKI was associated with an increased risk of mortality (aHR 1.90, 95% CI 1.76-2.06) and CKD stage 4 (aHR 1.33, 95% CI 1.11-1.59) including those that recovered from their AKI (mortality aHR 2.15, 95% CI 1.91-2.41; CKD aHR 1.73, 95% CI 1.37-2.19) and in those with stage 1 AKI (mortality aHR 1.90, 95% CI 1.74-2.07; CKD aHR 1.34, 95% CI 1.10-1.62). Outpatient AKI was also associated with with an increased risk of hospitalization (aHR 1.71, 95% CI 1.63-1.79), hospital AKI (aHR 2.14, 95% CI 1.93-2.37), and recurrent outpatient AKI (aHR 2.75, 95% CI 2.57-2.93). Conclusion: Outpatient AKI is common and is a risk factor for death, CKD, hospitalization, and recurrent AKI, including those with stage 1 AKI and those that recover.
Subjects/Keywords: acute kidney injury; chronic kidney disease; nephrology
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APA ·
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APA (6th Edition):
Leither, M. (2017). Outpatient Acute Kidney Injury Increases Risk of Mortality and Chronic Kidney Disease. (Masters Thesis). University of Minnesota. Retrieved from http://hdl.handle.net/11299/198958
Chicago Manual of Style (16th Edition):
Leither, Maxwell. “Outpatient Acute Kidney Injury Increases Risk of Mortality and Chronic Kidney Disease.” 2017. Masters Thesis, University of Minnesota. Accessed February 20, 2020.
http://hdl.handle.net/11299/198958.
MLA Handbook (7th Edition):
Leither, Maxwell. “Outpatient Acute Kidney Injury Increases Risk of Mortality and Chronic Kidney Disease.” 2017. Web. 20 Feb 2020.
Vancouver:
Leither M. Outpatient Acute Kidney Injury Increases Risk of Mortality and Chronic Kidney Disease. [Internet] [Masters thesis]. University of Minnesota; 2017. [cited 2020 Feb 20].
Available from: http://hdl.handle.net/11299/198958.
Council of Science Editors:
Leither M. Outpatient Acute Kidney Injury Increases Risk of Mortality and Chronic Kidney Disease. [Masters Thesis]. University of Minnesota; 2017. Available from: http://hdl.handle.net/11299/198958
McMaster University
7.
Khalili, Hadiseh.
SHROOM3 IN THE KIDNEY.
Degree: MSc, 2015, McMaster University
URL: http://hdl.handle.net/11375/17413
► Chronic kidney disease (CKD), defined as an irreversible reduction in glomerular filtration rate, is a large public health concern. Dissecting the genetic components of CKD…
(more)
▼ Chronic kidney disease (CKD), defined as an irreversible reduction in glomerular filtration rate, is a large public health concern. Dissecting the genetic components of CKD is required to improve our understanding of disease pathogenesis. Researchers have identified that SHROOM3, has very high associations with kidney disease and function. Shroom3 encodes an actin-binding protein important in regulating cell and tissue morphogenesis. However, there is a lack of evidence supporting a role for Shroom3 in kidney function or disease. Here, I investigated the developmental and functional role of Shroom3 in the mammalian kidney. For the first time, I described the expression pattern of Shroom3 in the embryonic and adult mouse kidneys. By performing in situ hybridization and immunohistochemistry, I demonstrated that Shroom3 is expressed in the condensing mesenchyme, podocytes, and collecting ducts. I further showed that Shroom3 protein is localized in the foot processes of podocytes, utilizing immunogold labeling and transmission electron microscopy. In order to uncover a potential role of Shroom3 in the kidney, we utilized Shroom3 knockout mice. Shroom3 mutants demonstrated marked glomerular abnormalities including cystic and degenerating glomeruli, and reduced glomerular number. Scanning and transmission electron microscopic analyses of Shroom3 mutant glomeruli revealed disruptions in podocyte morphology characterized by disorganized foot processes with less interdigitation and segmental foot processes effacement. Furthermore, immunofluorescence analysis of mutant kidneys revealed aberrant distribution of podocyte actin-associated proteins. Elucidating the underlying molecular mechanism of this abnormal podocyte architecture;
v
we demonstrated that in the absence of Shroom3, Rho kinase is mislocalized in the apical membrane of podocytes. As a result, mislocalized Rho kinase failed to phosphorylate non-muscle myosin and induce actomyosin contraction resulting in a patchy granular distribution of actin in the podocytes of Shroom3 mutants. Taken together, our findings established that Shroom3 is essential for proper actin organization in the podocytes through interaction with Rock. Furthermore, we took advantage of a haploinsufficiency phenotype of Shroom3 heterozygote adult mice and demonstrated these mice develop glomerulosclerosis and proteinuria. In conclusion, our studies provided evidence to support a role for Shroom3 in kidney development and disease and support the GWAS studies that suggested a correlation between SHROOM3 variants and kidney function in humans.
Thesis
Master of Science (MSc)
Advisors/Committee Members: Bridgewater, Darren, Medical Sciences.
Subjects/Keywords: Kidney; Glomerulus; Podocyte; Shroom3; CKD; Kidney Development
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APA (6th Edition):
Khalili, H. (2015). SHROOM3 IN THE KIDNEY. (Masters Thesis). McMaster University. Retrieved from http://hdl.handle.net/11375/17413
Chicago Manual of Style (16th Edition):
Khalili, Hadiseh. “SHROOM3 IN THE KIDNEY.” 2015. Masters Thesis, McMaster University. Accessed February 20, 2020.
http://hdl.handle.net/11375/17413.
MLA Handbook (7th Edition):
Khalili, Hadiseh. “SHROOM3 IN THE KIDNEY.” 2015. Web. 20 Feb 2020.
Vancouver:
Khalili H. SHROOM3 IN THE KIDNEY. [Internet] [Masters thesis]. McMaster University; 2015. [cited 2020 Feb 20].
Available from: http://hdl.handle.net/11375/17413.
Council of Science Editors:
Khalili H. SHROOM3 IN THE KIDNEY. [Masters Thesis]. McMaster University; 2015. Available from: http://hdl.handle.net/11375/17413
University of Alberta
8.
Aderibigbe, Ayodeji O.
The Regulation of Na+/H+ Exchanger Isoform 1 in Kidney
Cells.
Degree: PhD, Department of Biochemistry, 2016, University of Alberta
URL: https://era.library.ualberta.ca/files/cnv935307z
► The mammalian Na+/H+ exchanger isoform 1 (NHE1) is a ubiquitous plasma membrane protein that regulates intracellular pH by removing a proton in exchange for extracellular…
(more)
▼ The mammalian Na+/H+ exchanger isoform 1 (NHE1) is a
ubiquitous plasma membrane protein that regulates intracellular pH
by removing a proton in exchange for extracellular sodium. NHE1 is
expressed in many tissues including the kidney where it has been
demonstrated to play essential roles in pH and cell volume
regulation. It has also been implicated in some pathological
conditions of the kidney including ischemia and chronic kidney
diseases. NHE1 has a large 315 amino-acid cytosolic regulatory
domain that regulates the catalytic membrane domain. The cytosolic
domain is mainly regulated by phosphorylation and protein
interaction. This study examined how these regulatory mechanisms
regulate NHE1 in kidney cells. The activation of NHE1 in myocardial
cells by sustained intracellular acidosis (SIA) was shown to be
mediated by ERK1/2 phosphorylation. In this study, we also
demonstrate that SIA stimulates NHE1 activity in Mardin-Darby
Canine Kidney (MDCK) cells. To characterize how SIA stimulates NHE1
in kidney cells, wild-type and mutant NHE1 cDNAs were stably
expressed in MDCK cells and examined for activation and
phosphorylation in response to SIA. All the cDNAs had a L163F/G174S
mutation, which conferred a 100-fold resistance to EMD87580, an
NHE1-specific inhibitor. This allowed us to assay exogenous NHE1
activity while inhibiting endogenous activity with EMD87580 and
while inhibiting the NHE3 isoform of the Na⁺/H⁺ exchanger using the
isoform-specific inhibitor S3226. We demonstrated that the amino
acids Ser771, Ser776, Thr779, and Ser785 are important for NHE1
phosphorylation and activation after acute SIA. SIA also activated
ERK-dependent pathways in MDCK cells, and this was blocked by
treatment with the MEK inhibitor U0126. Treatment with U0126 also
blocked activation of NHE1 by SIA. These results suggest that acute
acidosis activates NHE1 in mammalian kidney cells and that in MDCK
cells this activation occurs through phosphorylation of a distinct
set of amino acids in the cytosolic regulatory tail of NHE1 by
ERK1/2. We also examined how protein interaction regulates NHE1
activity. Using affinity chromatography with the C-terminus of
NHE1, we determined the NHE1 binding proteins in the kidney which
includes 14-3-3 protein, heat shock proteins (Hsp90 and Hsp70) and
Na+/K+ ATPase. We also confirmed that 14-3-3 and heat shock
proteins bind to or regulate NHE1 but could not confirm that Na+/K+
ATPase binds to the intact protein. The Hsp90 inhibitor 17-AAG
decreased NHE1 activity and NHE1 phosphorylation in MDCK cells but
did not decrease protein levels. Additionally, 17-AAG decreased
phospho-AKT levels. Direct inhibition of AKT with MK2206 decreased
NHE1 activity, though this effect was not additive with the effect
of 17-AAG. These results are the first demonstration that in renal
cells, NHE1 is associated with several regulatory proteins
including Hsp90, and suggest that this interaction affect NHE1
function through altered phosphorylation of the protein via the AKT
kinase.
Subjects/Keywords: NHE1; Kidney; Acidosis
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APA ·
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APA (6th Edition):
Aderibigbe, A. O. (2016). The Regulation of Na+/H+ Exchanger Isoform 1 in Kidney
Cells. (Doctoral Dissertation). University of Alberta. Retrieved from https://era.library.ualberta.ca/files/cnv935307z
Chicago Manual of Style (16th Edition):
Aderibigbe, Ayodeji O. “The Regulation of Na+/H+ Exchanger Isoform 1 in Kidney
Cells.” 2016. Doctoral Dissertation, University of Alberta. Accessed February 20, 2020.
https://era.library.ualberta.ca/files/cnv935307z.
MLA Handbook (7th Edition):
Aderibigbe, Ayodeji O. “The Regulation of Na+/H+ Exchanger Isoform 1 in Kidney
Cells.” 2016. Web. 20 Feb 2020.
Vancouver:
Aderibigbe AO. The Regulation of Na+/H+ Exchanger Isoform 1 in Kidney
Cells. [Internet] [Doctoral dissertation]. University of Alberta; 2016. [cited 2020 Feb 20].
Available from: https://era.library.ualberta.ca/files/cnv935307z.
Council of Science Editors:
Aderibigbe AO. The Regulation of Na+/H+ Exchanger Isoform 1 in Kidney
Cells. [Doctoral Dissertation]. University of Alberta; 2016. Available from: https://era.library.ualberta.ca/files/cnv935307z
University of Alberta
9.
MacDonald, Christine, L.
Acute Kidney Injury After Heart Transplant in Children; Risk
Factors and Outcomes.
Degree: Master of Nursing, Faculty of Nursing, 2015, University of Alberta
URL: https://era.library.ualberta.ca/files/kp78gj706
► Background: Heart transplant is life-saving for children with end-stage congenital heart disease or acquired heart failure. Critical illness following transplantation can include acute kidney injury…
(more)
▼ Background: Heart transplant is life-saving for
children with end-stage congenital heart disease or acquired heart
failure. Critical illness following transplantation can include
acute kidney injury (AKI). There is little data on the epidemiology
of, risk factors for, or impact on outcomes of AKI after pediatric
heart transplant. Methods: Using secondary analysis of data from an
ongoing prospective cohort study, we evaluated 72 children (0- 5
yrs) who had a heart transplant between 2001 and 2012. We
evaluated: 1) postoperative AKI rate (defined by pRIFLE); 2) pre-,
intra-, and early postoperative AKI risk factors (days on waitlist,
inotrope use and ventilation pre-transplant, ECMO / ventricular
assist device at transplant, preoperative estimated glomerular
filtration rate (eGFR), ABO incompatibility, donor ischemic time,
peak intraoperative lactate, tacrolimus level early
postoperatively) using stepwise logistic regression; 3) effect of
AKI on short-term outcomes (duration of ventilation and length of
PICU stay). Results: AKI occurred in 73% of children. Independent
predictors of AKI were pre-transplant ventilation (OR 8.6, p=0.007)
and higher eGFR (p=0.032). Following adjustment, preoperative
inotrope significantly reduced the risk of AKI (OR 0.13, p=0.016).
Sixteen percent of children had a tacrolimus level >15 ug/L
on day 3 post-transplant and these children had more AKI than
children without (OR 7.8, p=0.086). Although not statistically
significant, automated model selection retained tacrolimus level
>15 ug/L as a predictor (using multiple different modeling
strategies). AKI resulted in longer ventilation days and ICU stay
(p=0.038 & p=0.004, respectively). Conclusion: AKI was
common after heart transplant and was associated with important
outcomes. As in other pediatric cardiac surgery populations, lower
preoperative GFR was protective against postoperative AKI; the role
of modified immune suppressive strategies in this context needs to
be further evaluated. Although not statistically significant,
elevated early postoperative tacrolimus is likely biologically
important in the prediction of AKI risk and needs further
evaluation in a larger cohort.
Subjects/Keywords: Heart; Kidney; Transplant
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APA (6th Edition):
MacDonald, Christine, L. (2015). Acute Kidney Injury After Heart Transplant in Children; Risk
Factors and Outcomes. (Masters Thesis). University of Alberta. Retrieved from https://era.library.ualberta.ca/files/kp78gj706
Chicago Manual of Style (16th Edition):
MacDonald, Christine, L. “Acute Kidney Injury After Heart Transplant in Children; Risk
Factors and Outcomes.” 2015. Masters Thesis, University of Alberta. Accessed February 20, 2020.
https://era.library.ualberta.ca/files/kp78gj706.
MLA Handbook (7th Edition):
MacDonald, Christine, L. “Acute Kidney Injury After Heart Transplant in Children; Risk
Factors and Outcomes.” 2015. Web. 20 Feb 2020.
Vancouver:
MacDonald, Christine L. Acute Kidney Injury After Heart Transplant in Children; Risk
Factors and Outcomes. [Internet] [Masters thesis]. University of Alberta; 2015. [cited 2020 Feb 20].
Available from: https://era.library.ualberta.ca/files/kp78gj706.
Council of Science Editors:
MacDonald, Christine L. Acute Kidney Injury After Heart Transplant in Children; Risk
Factors and Outcomes. [Masters Thesis]. University of Alberta; 2015. Available from: https://era.library.ualberta.ca/files/kp78gj706
10.
Madhvi Awasthi.
Counseling of kidney stone patients based on their
dietary pattern in the selected areas of district kangra
HP;.
Degree: 2013, INFLIBNET
URL: http://shodhganga.inflibnet.ac.in/handle/10603/10257
► The present study was planned and executed with the specific objectives to counsel the kidney stone patients based on their dietary pattern in the selected…
(more)
▼ The present study was planned and executed with the
specific objectives to counsel the kidney stone patients based on
their dietary pattern in the selected areas of district Kangra
(Himachal Pradesh). Out of total 130 selected kidney stone
patients, majority were males varying in their time of stone
diagnosis, symptoms of the disease, recurrence and inheritance of
stones and anthropometrical measurements. Majority of patients were
non-vegetarian with normal body weight. Their intake of macro
nutrient and water was lower than RDA while intake of minerals
(sodium, calcium and phosphorus) and oxalates was higher than RDA
which may be a contributory factor of stone formation. The
assessment of knowledge regarding general nutrition awareness,
knowledge regarding kidney stones and knowledge regarding nutrition
and kidney stone was done and nutrition education was also provided
to patients. There was positive correlation between literacy level
and general nutrition awareness and literacy level and nutrition
and kidney stones. Correlation study between literacy level and
gain in knowledge regarding kidney stones after nutrition education
was also positive. Overall comparison of mean gain and quantum of
improvement in knowledge of three categories revealed that there
was more gain and improvement of knowledge regarding nutrition and
kidney stones and more than half of respondents had gain in
knowledge categorized as high. The study revealed that if patients
are counseled and guided properly in the selection of appropriate
foods, much of recurrence and complications of kidneys can be
prevented thereby improving the general health. Proper nutrition
can support healthy kidney function and may discourage stone
formation. newline
Advisors/Committee Members: Dr. (Mrs.) S.R. Malhotra.
Subjects/Keywords: Dietary; kidney stone
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APA (6th Edition):
Awasthi, M. (2013). Counseling of kidney stone patients based on their
dietary pattern in the selected areas of district kangra
HP;. (Thesis). INFLIBNET. Retrieved from http://shodhganga.inflibnet.ac.in/handle/10603/10257
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Awasthi, Madhvi. “Counseling of kidney stone patients based on their
dietary pattern in the selected areas of district kangra
HP;.” 2013. Thesis, INFLIBNET. Accessed February 20, 2020.
http://shodhganga.inflibnet.ac.in/handle/10603/10257.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Awasthi, Madhvi. “Counseling of kidney stone patients based on their
dietary pattern in the selected areas of district kangra
HP;.” 2013. Web. 20 Feb 2020.
Vancouver:
Awasthi M. Counseling of kidney stone patients based on their
dietary pattern in the selected areas of district kangra
HP;. [Internet] [Thesis]. INFLIBNET; 2013. [cited 2020 Feb 20].
Available from: http://shodhganga.inflibnet.ac.in/handle/10603/10257.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Awasthi M. Counseling of kidney stone patients based on their
dietary pattern in the selected areas of district kangra
HP;. [Thesis]. INFLIBNET; 2013. Available from: http://shodhganga.inflibnet.ac.in/handle/10603/10257
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Addis Ababa University
11.
Afewerki, Bekele.
Project paper on: The effect of zinc exposure on the histology of liver and kidney
.
Degree: 2014, Addis Ababa University
URL: http://etd.aau.edu.et/dspace/handle/123456789/5190
► Summary Zinc is one of the most important trace elements in the body that participates in the biological function of several proteins and enzymes. Despite…
(more)
▼ Summary
Zinc is one of the most important trace elements in the body that participates in the biological function of several proteins and enzymes. Despite the fact that small quantity of zinc is required for the normal development and metabolism, its effect is toxic when a certain concentration is exceeded. The aim of this paper is to review scientific literatures on the protective and toxic effect of zinc exposure on the histology of liver and
kidney. The studies were evaluated in relation to dose of zinc, zinc combination with other toxic metals, duration of exposure, type of animal model used, method of study used and parameter used to measure protective effect of zinc against toxic metals and toxic effect of zinc alone. The different literatures reviewed in this paper used rats, mice, lambs, fishes, ducks. The literature reviewed showed that Zn administration together with Al, Cd, as well as organic solvent such as ethanol has a protective effect against Al, Cd induced toxicity in liver and
kidney tissues and ethanol induced toxicity in liver tissue. On the other hand, as zinc dose level and duration of the exposure increases, it act as toxic metal to histology of liver and
kidney.
Advisors/Committee Members: Girma Seyoum (PhD) (advisor).
Subjects/Keywords: zinc; liver; kidney
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APA (6th Edition):
Afewerki, B. (2014). Project paper on: The effect of zinc exposure on the histology of liver and kidney
. (Thesis). Addis Ababa University. Retrieved from http://etd.aau.edu.et/dspace/handle/123456789/5190
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Afewerki, Bekele. “Project paper on: The effect of zinc exposure on the histology of liver and kidney
.” 2014. Thesis, Addis Ababa University. Accessed February 20, 2020.
http://etd.aau.edu.et/dspace/handle/123456789/5190.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Afewerki, Bekele. “Project paper on: The effect of zinc exposure on the histology of liver and kidney
.” 2014. Web. 20 Feb 2020.
Vancouver:
Afewerki B. Project paper on: The effect of zinc exposure on the histology of liver and kidney
. [Internet] [Thesis]. Addis Ababa University; 2014. [cited 2020 Feb 20].
Available from: http://etd.aau.edu.et/dspace/handle/123456789/5190.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Afewerki B. Project paper on: The effect of zinc exposure on the histology of liver and kidney
. [Thesis]. Addis Ababa University; 2014. Available from: http://etd.aau.edu.et/dspace/handle/123456789/5190
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
University of California – San Francisco
12.
Du, David.
Effects of Periodontal Treatment on Diabetes and Chronic Kidney Disease.
Degree: Oral and Craniofacial Sciences, 2015, University of California – San Francisco
URL: http://www.escholarship.org/uc/item/02k290s0
► Introduction: Periodontitis affects 47.2% of the US population and 64% of adults over 65 (20). Chronic kidney disease (CKD) affects an estimated 13% to 16%…
(more)
▼ Introduction: Periodontitis affects 47.2% of the US population and 64% of adults over 65 (20). Chronic kidney disease (CKD) affects an estimated 13% to 16% of the US population and accounts for 27% of Medicare spending in 2007 (1). The central question (PICO) in this study is: In a population of patients with compromised renal function, is there a difference in reduction of systemic markers of HBA1C and serum albumin between the intensive periodontal intervention and community treatment group? Materials and Methods: This is an ongoing unblinded, randomized, controlled pilot trial with two intent-to-treat treatment arms: immediate intensive periodontal therapy or delayed intensive periodontal therapy. The goal of this study was to test the feasibility of conducting this trial among an underserved population (mostly poor and/or low literacy), and to determine the variability of HbA1c levels and albumin/creatinine ratio (A/C%) in response to intensive periodontal therapy over a 4-month period among participants with both CKD and significant periodontal disease. Smoking habits were also reported in a survey taken at baseline.Results: In the treatment group, HbA1c at baseline (6.35%) did not show any difference at 4 months (6.4%) with a change of 0.05. In the control group, HbA1c was virtually the same at baseline (6.68%) and 4 months (6.5%) with a change of -.018. The A/C % ratio for the treatment group at baseline (674.80 mg/g) and at 4 months (639.68 mg/g) showed a change of 167.47. In the control group, the A/C % ratio at baseline (629.40 mg/g) and at 4 months (302.75 mg/g) showed a change of -232.97. Although the changes in A/C % ratio seemed dramatic, their p-values were 0.5625 and 1.000, respectively, indicating no statistical difference. Correlation for smokers could not be performed due to low sample size of 5 subjects in treatment and 2 in control groups.Conclusion: Given the small sample size, this study showed no statistically significant reduction in systemic markers of diabetes as represented by HbA1c or chronic renal disease as represented by albumin/creatinine % ratio. Since this is an ongoing study, when it reaches 51 subjects, there may be a statistically significant improvement in systemic markers of HbA1c and albumin/creatinine % levels. Finally, when all the analysis of the blood samples taken are completed on 51 subjects at 12 months, there will be much more information that can be used to answer the research question.
Subjects/Keywords: Dentistry; Kidney; Periodontitis
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Manager
APA (6th Edition):
Du, D. (2015). Effects of Periodontal Treatment on Diabetes and Chronic Kidney Disease. (Thesis). University of California – San Francisco. Retrieved from http://www.escholarship.org/uc/item/02k290s0
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Du, David. “Effects of Periodontal Treatment on Diabetes and Chronic Kidney Disease.” 2015. Thesis, University of California – San Francisco. Accessed February 20, 2020.
http://www.escholarship.org/uc/item/02k290s0.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Du, David. “Effects of Periodontal Treatment on Diabetes and Chronic Kidney Disease.” 2015. Web. 20 Feb 2020.
Vancouver:
Du D. Effects of Periodontal Treatment on Diabetes and Chronic Kidney Disease. [Internet] [Thesis]. University of California – San Francisco; 2015. [cited 2020 Feb 20].
Available from: http://www.escholarship.org/uc/item/02k290s0.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Du D. Effects of Periodontal Treatment on Diabetes and Chronic Kidney Disease. [Thesis]. University of California – San Francisco; 2015. Available from: http://www.escholarship.org/uc/item/02k290s0
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
University of Manitoba
13.
Hwang, Sun-Young.
Role of hyperhomocysteinemia in the regulation of oxidative stress and inflammatory responses in the kidney: protective effect of folic acid supplementation.
Degree: Animal Science, 2011, University of Manitoba
URL: http://hdl.handle.net/1993/5066
► Hyperhomocysteinemia, a condition of elevated blood homocysteine (Hcy) level, is an independent risk factor for cardiovascular disease. Folic acid supplementation can effectively reduce blood Hcy…
(more)
▼ Hyperhomocysteinemia, a condition of elevated blood homocysteine (Hcy) level, is an independent risk factor for cardiovascular disease. Folic acid supplementation can effectively reduce blood Hcy levels. Recent studies have demonstrated that hyperhomocysteinemia is also associated with
kidney disease. However, the underlying mechanisms remain unclear. The overall objective of the study was to investigate the biochemical and molecular mechanisms of Hcy-induced
kidney injury and the effect of folic acid supplementation on Hcy-induced
kidney injury.
Hyperhomocysteinemia was induced in Sprague-Dawley rats by feeding a high-methionine diet for 12 weeks. An elevation of serum total Hcy level was observed in hyperhomocysteinemic rats. Hyperhomocysteinemia-induced superoxide anion production via nicotinamide adenine dinucleotide phosphate (NADPH) oxidase activation resulted in oxidative stress in the
kidney. Reduction of oxidative stress by inhibiting superoxide anion production effectively ameliorated hyperhomocysteinemia-induced
kidney injury.
Inflammatory responses such as increased chemokine expression have been implicated as one of the mechanisms of
kidney disease. Monocyte chemoattractant protein-1 (MCP-1) is a potent chemokine that is involved in the inflammatory response in
kidney disease. Nuclear factor-kappa B (NF-kappaB) plays an important role in upregulation of MCP-1 expression. We investigated the effect of hyperhomocysteinemia on MCP-1 expression and the molecular mechanism responsible for such an effect in rat kidneys as well as in human
kidney proximal tubular cells.
Advisors/Committee Members: O, Karmin (Animal Science) (supervisor), Guenter, Bill (Animal Science) House, Jamnes (Animal Science) Suh, Miyoung (Human Nutritional Science) (examiningcommittee).
Subjects/Keywords: Homocysteine; Kidney Disease
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Manager
APA (6th Edition):
Hwang, S. (2011). Role of hyperhomocysteinemia in the regulation of oxidative stress and inflammatory responses in the kidney: protective effect of folic acid supplementation. (Thesis). University of Manitoba. Retrieved from http://hdl.handle.net/1993/5066
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Hwang, Sun-Young. “Role of hyperhomocysteinemia in the regulation of oxidative stress and inflammatory responses in the kidney: protective effect of folic acid supplementation.” 2011. Thesis, University of Manitoba. Accessed February 20, 2020.
http://hdl.handle.net/1993/5066.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Hwang, Sun-Young. “Role of hyperhomocysteinemia in the regulation of oxidative stress and inflammatory responses in the kidney: protective effect of folic acid supplementation.” 2011. Web. 20 Feb 2020.
Vancouver:
Hwang S. Role of hyperhomocysteinemia in the regulation of oxidative stress and inflammatory responses in the kidney: protective effect of folic acid supplementation. [Internet] [Thesis]. University of Manitoba; 2011. [cited 2020 Feb 20].
Available from: http://hdl.handle.net/1993/5066.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Hwang S. Role of hyperhomocysteinemia in the regulation of oxidative stress and inflammatory responses in the kidney: protective effect of folic acid supplementation. [Thesis]. University of Manitoba; 2011. Available from: http://hdl.handle.net/1993/5066
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
University of Manitoba
14.
Choi, Nora.
Study of iron pathophysiology for early diagnosis of acute kidney injury secondary to ischemia reperfusion injury following cardiopulmonary bypass.
Degree: Immunology, 2017, University of Manitoba
URL: http://hdl.handle.net/1993/32763
► Currently, there are no successful therapies proven to ameliorate acute kidney injury (AKI). AKI secondary to ischemia reperfusion injury (IRI) leads to increased morbidity and…
(more)
▼ Currently, there are no successful therapies proven to ameliorate acute
kidney injury (AKI). AKI secondary to ischemia reperfusion injury (IRI) leads to increased morbidity and mortality. The role of iron sequestration throughout cardiac surgery remains unclear, however it may be an important modifier of renal ischemia reperfusion injury. The primary goal was to characterize iron regulatory pathways in a prospective observational cohort of adult cardiac surgery patients in the context of clinical AKI predictors and in relation to clinical prediction alone. The secondary goal was to evaluate potential proteins that may act as non-invasive biomarkers for the early detection of AKI, including urine hepcidin-25, serum ferritin, serum transferrin saturation (TSAT) and urine lactotransferrin. We found that urine hepcidin-25 at postoperative day 1 and serum ferritin and TSAT at 1 hour into cardiopulmonary bypass were independent predictors of AKI avoidance on multivariate analysis, enhancing clinical prediction alone.
Advisors/Committee Members: Ho, Julie (Immunology) (supervisor), HayGlass, Kent (Immunology).
Subjects/Keywords: Acute kidney injury
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APA ·
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to Zotero / EndNote / Reference
Manager
APA (6th Edition):
Choi, N. (2017). Study of iron pathophysiology for early diagnosis of acute kidney injury secondary to ischemia reperfusion injury following cardiopulmonary bypass. (Masters Thesis). University of Manitoba. Retrieved from http://hdl.handle.net/1993/32763
Chicago Manual of Style (16th Edition):
Choi, Nora. “Study of iron pathophysiology for early diagnosis of acute kidney injury secondary to ischemia reperfusion injury following cardiopulmonary bypass.” 2017. Masters Thesis, University of Manitoba. Accessed February 20, 2020.
http://hdl.handle.net/1993/32763.
MLA Handbook (7th Edition):
Choi, Nora. “Study of iron pathophysiology for early diagnosis of acute kidney injury secondary to ischemia reperfusion injury following cardiopulmonary bypass.” 2017. Web. 20 Feb 2020.
Vancouver:
Choi N. Study of iron pathophysiology for early diagnosis of acute kidney injury secondary to ischemia reperfusion injury following cardiopulmonary bypass. [Internet] [Masters thesis]. University of Manitoba; 2017. [cited 2020 Feb 20].
Available from: http://hdl.handle.net/1993/32763.
Council of Science Editors:
Choi N. Study of iron pathophysiology for early diagnosis of acute kidney injury secondary to ischemia reperfusion injury following cardiopulmonary bypass. [Masters Thesis]. University of Manitoba; 2017. Available from: http://hdl.handle.net/1993/32763
University of Newcastle
15.
Boudville, Neil.
Outcomes following living kidney donation.
Degree: 2018, University of Newcastle
URL: http://hdl.handle.net/1959.13/1385432
► Higher Doctorate - Doctor of Medicine (DMed)
The research that forms the content of this thesis has been performed over the last 14 years and…
(more)
▼ Higher Doctorate - Doctor of Medicine (DMed)
The research that forms the content of this thesis has been performed over the last 14 years and is an ongoing line of research that I am likely to perform for the rest of my career. While a great deal of research has been performed on transplant recipients, it became clear to me 14 years ago that there was limited good research on the outcomes of people who donate their kidneys to people with end-stage kidney disease (except for short-term outcomes in the immediate post-operative period). I subsequently embarked on a research path that commenced with a series of systematic reviews, followed by cross-sectional studies and finally to the commencement of a large prospective observational study to explore in more detail the outcomes of living kidney donors. This dissertation reviews my research to date, with the large prospective study still years away from completion. My research efforts have not included commercial donors, who likely have quite different outcomes. There are a number of important patient-level outcomes that I have explored and so I have divided this thesis into chapters based upon groups of these outcomes. Within the chapters I follow a primarily temporal order for my research and describe some other key publications from other research groups.
Advisors/Committee Members: University of Newcastle. Faculty of Health & Medicine, School of Medicine and Public Health.
Subjects/Keywords: chronic kidney disease; kidney transplantation; living kidney transplantation; living donor
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APA (6th Edition):
Boudville, N. (2018). Outcomes following living kidney donation. (Thesis). University of Newcastle. Retrieved from http://hdl.handle.net/1959.13/1385432
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Boudville, Neil. “Outcomes following living kidney donation.” 2018. Thesis, University of Newcastle. Accessed February 20, 2020.
http://hdl.handle.net/1959.13/1385432.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Boudville, Neil. “Outcomes following living kidney donation.” 2018. Web. 20 Feb 2020.
Vancouver:
Boudville N. Outcomes following living kidney donation. [Internet] [Thesis]. University of Newcastle; 2018. [cited 2020 Feb 20].
Available from: http://hdl.handle.net/1959.13/1385432.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Boudville N. Outcomes following living kidney donation. [Thesis]. University of Newcastle; 2018. Available from: http://hdl.handle.net/1959.13/1385432
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
University of Western Ontario
16.
Naylor, Kyla L.
Epidemiology of Fracture in Adults with Kidney Disease.
Degree: 2015, University of Western Ontario
URL: https://ir.lib.uwo.ca/etd/3003
► Fractures are a global health concern, leading to morbidity and mortality. Individuals with reduced kidney function experience bone mineral metabolism changes which can increase fracture…
(more)
▼ Fractures are a global health concern, leading to morbidity and mortality. Individuals with reduced kidney function experience bone mineral metabolism changes which can increase fracture risk. Yet, there is little consensus on the fundamentals: prediction, incidence, risk factors, and screening of fractures in kidney disease patients. This thesis addressed these critical areas helping decrease the health burden of fracture in this unique population.
This research used data from the Canadian Multicentre Osteoporosis Study (CaMos) to examine individuals with chronic kidney disease (CKD) (n=320). CaMos is a national longitudinal study designed to collect information on fractures. To examine kidney transplant recipients data from Ontario administrative healthcare databases was used (n=4821). The predictive ability of the Fracture Risk Assessment Tool (FRAX) in individuals with CKD was evaluated using area under the receiver operator characteristic curves and survival analyses. The incidence and risk factors for fracture in kidney transplant recipients were assessed using incidence rates and Cox hazard regression analysis.
The first manuscript systematically summarized the incidence and risk factors for fracture in kidney transplant recipients; fracture incidence and risk factors were variable across studies.
The second manuscript examined the predictive value of FRAX in individuals with CKD compared to individuals with normal kidney function. The discriminative ability of FRAX for fracture prediction was comparable in both groups.
The third manuscript examined the incidence of fracture in kidney transplant recipients. The cumulative incidence of fracture was low with approximately 2% sustaining a hip fracture over 10-years.
The fourth manuscript examined risk factors for fracture in kidney transplant recipients. Transplant-specific risk factors (i.e., diabetes or cystic kidney disease as the cause of end-stage renal disease and donor age) and general risk factors (i.e., older recipient age and female sex) were significantly associated with fractures.
The fifth manuscript examined the frequency and variability in bone mineral density (BMD) testing across Ontario transplant centres. Over half of kidney transplant recipients received at least one BMD and the ordering of BMD tests varied widely by centre – from 15% to 92%.
Results can be used to improve prognostication, advance clinical guidelines, clarify fracture incidence, and guide informed consent.
Subjects/Keywords: fracture; kidney disease; chronic kidney disease; kidney transplant recipient; epidemiology; bone; Epidemiology
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Manager
APA (6th Edition):
Naylor, K. L. (2015). Epidemiology of Fracture in Adults with Kidney Disease. (Thesis). University of Western Ontario. Retrieved from https://ir.lib.uwo.ca/etd/3003
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Naylor, Kyla L. “Epidemiology of Fracture in Adults with Kidney Disease.” 2015. Thesis, University of Western Ontario. Accessed February 20, 2020.
https://ir.lib.uwo.ca/etd/3003.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Naylor, Kyla L. “Epidemiology of Fracture in Adults with Kidney Disease.” 2015. Web. 20 Feb 2020.
Vancouver:
Naylor KL. Epidemiology of Fracture in Adults with Kidney Disease. [Internet] [Thesis]. University of Western Ontario; 2015. [cited 2020 Feb 20].
Available from: https://ir.lib.uwo.ca/etd/3003.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Naylor KL. Epidemiology of Fracture in Adults with Kidney Disease. [Thesis]. University of Western Ontario; 2015. Available from: https://ir.lib.uwo.ca/etd/3003
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
University of Sydney
17.
Hanson, Camilla Sara.
Improving Access and Outcomes in Living Kidney Donor Transplantation
.
Degree: 2017, University of Sydney
URL: http://hdl.handle.net/2123/17370
► Shortages in deceased organ donation have necessitated widespread acceptance of living donor kidney transplantation, which offers better outcomes in terms of graft survival, life expectancy…
(more)
▼ Shortages in deceased organ donation have necessitated widespread acceptance of living donor kidney transplantation, which offers better outcomes in terms of graft survival, life expectancy and quality of life for many patients with end-stage kidney disease, compared with deceased donor kidney transplantation and dialysis. However, there are barriers and challenges that exist in the current practice of living kidney donor transplantation. Overall, the rates of living kidney donor transplantation have decreased or plateaued, with ethic and socio-economic disparities in access to living kidney donation reported in many countries that remain largely unexplained. Living donors must accept risks associated with undergoing nephrectomy, yet the evidence on the long-term risks of living kidney donation remains uncertain. In response, there have been efforts to identify and describe the barriers and disparities in living kidney donor transplantation, improve the pathway for living donors and to assess a range of outcomes for living donors. A comprehensive understanding of the values, beliefs, experiences, priorities and preferences of the key stakeholders, including donors and health professionals involved in their care is needed to ensure that research; clinical practice and policy in living kidney donation address their needs and priorities. This is a thesis by publication containing published and submitted work. The aims of the studies included in this thesis are: 1) To identify and describe the beliefs, attitudes and expectations of patients with CKD regarding living kidney donation. 2) To describe kidney donors’ experiences of the evaluation process, and the motivations and challenges to sustaining commitment prior to donation. 3) To describe nephrologists’ perspectives on barriers to living kidney donation and disparities in access to living kidney donor transplantation. 4) To identify living kidney donors’ priorities for outcomes and describe the reasons for their choices. 5) To determine the characteristics and heterogeneity of outcomes reported in randomised trials and observational studies in adult living kidney donors
Subjects/Keywords: Kidney disease;
kidney transplant;
living kidney donor;
qualitative;
nominal group technique;
outcomes
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APA (6th Edition):
Hanson, C. S. (2017). Improving Access and Outcomes in Living Kidney Donor Transplantation
. (Thesis). University of Sydney. Retrieved from http://hdl.handle.net/2123/17370
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Hanson, Camilla Sara. “Improving Access and Outcomes in Living Kidney Donor Transplantation
.” 2017. Thesis, University of Sydney. Accessed February 20, 2020.
http://hdl.handle.net/2123/17370.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Hanson, Camilla Sara. “Improving Access and Outcomes in Living Kidney Donor Transplantation
.” 2017. Web. 20 Feb 2020.
Vancouver:
Hanson CS. Improving Access and Outcomes in Living Kidney Donor Transplantation
. [Internet] [Thesis]. University of Sydney; 2017. [cited 2020 Feb 20].
Available from: http://hdl.handle.net/2123/17370.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Hanson CS. Improving Access and Outcomes in Living Kidney Donor Transplantation
. [Thesis]. University of Sydney; 2017. Available from: http://hdl.handle.net/2123/17370
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
University of Edinburgh
18.
Street, Jonathan Mark.
Exosomal proteome as a source of biomarkers for human disease.
Degree: PhD, 2011, University of Edinburgh
URL: http://hdl.handle.net/1842/5895
► Exosomes are small lipid membrane bound vesicles formed as part of the endosomal pathway and released into the extracellular space following fusion of late endosomes…
(more)
▼ Exosomes are small lipid membrane bound vesicles formed as part of the endosomal pathway and released into the extracellular space following fusion of late endosomes with the plasma membrane. Exosomes have been shown to have a variety of biological roles and may represent a novel source of disease biomarkers. The objectives of this project were to develop a panel of techniques for identifying exosomes in human urine then establish an in vitro model to determine whether exosomes change with cellular activation. We then used the techniques developed with human urine to determine whether human cerebrospinal fluid (CSF) contains exosomes and applied a mass spectrometry based approach to characterise the exosomal proteome. We used western blot for three exosomal markers (tsg101, CD24 and flotillin- 1), isopycnic centrifugation on a sucrose density gradient and direct visualisation using transmission electron microscopy (TEM) to verify the presence of exosomes. Using GeLC-MS/MS, 88 proteins were identified in the urinary exosomes. Several of these proteins could be linked to diseases and specific sections of the nephron. A murine cortical collecting duct cell line was used to model exosome release into the urine. Firstly, exosome release was verified using the approach developed in the urine. Stimulation of the cells with desmopressin caused an increase in the presence of aquaporin 2 in the exosomes. This increase reflected a similar change in the cells and occurred over a similar time course. This supports the hypothesis that the exosomes reflect the state of the kidney cells. In contrast, stimulation with cisplatin did not alter the presence of Fetuin-A, a proposed biomarker of cisplatin-induced acute kidney injury, in exosomes and this was consistent with no change in Fetuin- A expression in the cells. The released exosomes may act as mediators of communication to other cells. Following incubation of mCCD cells with AQP2 containing exosomes AQP2 in the cell lysate was increased indicating interaction between the cells and exosomes and potentially internalisation. Exosomes have been shown to be released by neuronal cells in vitro. We identified exosomes in the CSF of humans using western blot for known exosomal markers, density determination and direct visualisation with TEM and Immuno-TEM using an antibody specific for the exosomal marker flotillin-1. Label-free quantitative mass spectrometry was used to compare multiple CSF samples. On a whole protein analysis 86% of the proteins identified varied by less than 2-fold in comparison to the average across samples. On a tryptic peptide analysis 75% of the peptides identified varied by less than 2-fold in comparison with the average across samples. We have demonstrated exosomes are present in urine, CSF and mCCD cell conditioned media. In the mCCD cell derived exosomes we have demonstrated that following stimulation the proteome of the exosomes changes and that this change reflects the change seen in the cells. For the urinary and CSF exosomes we have characterised their…
Subjects/Keywords: 616.6; exosomes; proteomics; CNS; kidney
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APA (6th Edition):
Street, J. M. (2011). Exosomal proteome as a source of biomarkers for human disease. (Doctoral Dissertation). University of Edinburgh. Retrieved from http://hdl.handle.net/1842/5895
Chicago Manual of Style (16th Edition):
Street, Jonathan Mark. “Exosomal proteome as a source of biomarkers for human disease.” 2011. Doctoral Dissertation, University of Edinburgh. Accessed February 20, 2020.
http://hdl.handle.net/1842/5895.
MLA Handbook (7th Edition):
Street, Jonathan Mark. “Exosomal proteome as a source of biomarkers for human disease.” 2011. Web. 20 Feb 2020.
Vancouver:
Street JM. Exosomal proteome as a source of biomarkers for human disease. [Internet] [Doctoral dissertation]. University of Edinburgh; 2011. [cited 2020 Feb 20].
Available from: http://hdl.handle.net/1842/5895.
Council of Science Editors:
Street JM. Exosomal proteome as a source of biomarkers for human disease. [Doctoral Dissertation]. University of Edinburgh; 2011. Available from: http://hdl.handle.net/1842/5895
University of Leicester
19.
Hosgood, Sarah Anne.
Normothermic perfusion in renal transplantation.
Degree: PhD, 2012, University of Leicester
URL: http://hdl.handle.net/2381/10924
► One of the main causes of early graft dysfunction in kidney transplantation is ischaemia reperfusion (I/R) injury. This unavoidable event occurs immediately when oxygenated blood…
(more)
▼ One of the main causes of early graft dysfunction in kidney transplantation is ischaemia reperfusion (I/R) injury. This unavoidable event occurs immediately when oxygenated blood is re-introduced into the transplanted kidney. Its severity is influenced by many predetermined factors. However, the condition under which an organ is preserved has a significant bearing on the outcome. Traditionally, organs are preserved using hypothermic temperatures, to reduce metabolism and the requirement for oxygen. Although practical and simple, hypothermic conditions are not entirely favourable and over time the depletion of energy substrates causes substantial cellular injury. This is thought to be a particular problem in kidneys from marginal donors, which are often exposed to a period of warm ischaemia (WI) prior to retrieval. The aim of this thesis was to determine the effects of varying degrees of the combined insults of warm and cold ischaemic injury and to develop a technique of normothermic perfusion (NP) to reduce ischaemic injury. The effects were assessed using ex-vivo and in-vivo porcine kidney models before translation of NP into clinical practice for marginal donor kidneys. This research demonstrated that prolonging the hypothermic preservation period after a minimal and a substantial degree of WI injury increased the severity of acute I/R injury and graft dysfunction. A short period of NP after hypothermic preservation was able to resuscitate the kidney, replenish ATP and reverse some of the detrimental effects of cold ischaemic injury. When translated into an autotransplant model, NP was found to be a safe and feasible method of preservation. NP was then adapted for use in clinical practice for kidneys from marginal donors. This first in man clinical series of 15 cases has demonstrated the safety and feasibility of NP for marginal kidneys. Although, the high rate of initial graft function is notable, further comparative studies are required to assess the effects on delayed graft function.
Subjects/Keywords: 617.4610592; Kidney; Preservation; Normothermic
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APA (6th Edition):
Hosgood, S. A. (2012). Normothermic perfusion in renal transplantation. (Doctoral Dissertation). University of Leicester. Retrieved from http://hdl.handle.net/2381/10924
Chicago Manual of Style (16th Edition):
Hosgood, Sarah Anne. “Normothermic perfusion in renal transplantation.” 2012. Doctoral Dissertation, University of Leicester. Accessed February 20, 2020.
http://hdl.handle.net/2381/10924.
MLA Handbook (7th Edition):
Hosgood, Sarah Anne. “Normothermic perfusion in renal transplantation.” 2012. Web. 20 Feb 2020.
Vancouver:
Hosgood SA. Normothermic perfusion in renal transplantation. [Internet] [Doctoral dissertation]. University of Leicester; 2012. [cited 2020 Feb 20].
Available from: http://hdl.handle.net/2381/10924.
Council of Science Editors:
Hosgood SA. Normothermic perfusion in renal transplantation. [Doctoral Dissertation]. University of Leicester; 2012. Available from: http://hdl.handle.net/2381/10924
20.
Uchiyama, Takanobu; Takata, Shunsuke; Ishikawa, Hiroyuki.
Altered dynamics in the renal lymphatic circulation of type 1 and type 2 diabetic mice. : Altered dynamics in the renal lymphatic circulation of type 1 and type 2 diabetic mice.
Degree: 博士（歯学）, 2014, Fukuoka Dental College / 福岡歯科大学
URL: http://id.nii.ac.jp/1167/00000009/
► The dynamics of the renal lymphatic circulation in diabetic nephropathy is not fully elucidated. The present study evaluated the effect of diabetic nephropathy on the…
(more)
▼ The dynamics of the renal lymphatic circulation in diabetic nephropathy is not fully elucidated. The present study evaluated the effect of diabetic nephropathy on the renal lymphatic circulation in streptozotocin (STZ)-induced type 1 diabetic mice (ICR-STZ) and in type 2 diabetic KK/Ta mice which were fed a high fat diet (KK/Ta-HF). The diabetic mouse kidneys developed edema because of the nephropathy. In control mice renal lymphatic vessels distributed in the cortex but rarely in the medulla while in ICR-STZ and KK/Ta-HF mice, there were many lymphatic vessels with small lumen in both cortex and medulla. Total numbers and areas of renal blood vessels in the diabetic mice were similar to those in the controls while the total numbers and areas of renal lymphatic vessels were larger in diabetic mice than in the controls. There were statistically significant differences in the numbers of lymphatic vessels with diameters of 50-100 µm between the ICR-STZ and the control ICR mice, and in the numbers of lymphatic capillaries with diameters smaller than 50 µm between the KK/Ta-HF and the control KK/Ta mice. The diabetic nephropathy may induce the lymphangiogenesis or result in at least the renal lymphatic vessel expansion.
2013年度
Subjects/Keywords: diabetes; kidney; lymphangiogenesis; lymphatics; nephropathy
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APA (6th Edition):
Uchiyama, Takanobu; Takata, Shunsuke; Ishikawa, H. (2014). Altered dynamics in the renal lymphatic circulation of type 1 and type 2 diabetic mice. : Altered dynamics in the renal lymphatic circulation of type 1 and type 2 diabetic mice. (Thesis). Fukuoka Dental College / 福岡歯科大学. Retrieved from http://id.nii.ac.jp/1167/00000009/
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Chicago Manual of Style (16th Edition):
Uchiyama, Takanobu; Takata, Shunsuke; Ishikawa, Hiroyuki. “Altered dynamics in the renal lymphatic circulation of type 1 and type 2 diabetic mice. : Altered dynamics in the renal lymphatic circulation of type 1 and type 2 diabetic mice.” 2014. Thesis, Fukuoka Dental College / 福岡歯科大学. Accessed February 20, 2020.
http://id.nii.ac.jp/1167/00000009/.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
MLA Handbook (7th Edition):
Uchiyama, Takanobu; Takata, Shunsuke; Ishikawa, Hiroyuki. “Altered dynamics in the renal lymphatic circulation of type 1 and type 2 diabetic mice. : Altered dynamics in the renal lymphatic circulation of type 1 and type 2 diabetic mice.” 2014. Web. 20 Feb 2020.
Vancouver:
Uchiyama, Takanobu; Takata, Shunsuke; Ishikawa H. Altered dynamics in the renal lymphatic circulation of type 1 and type 2 diabetic mice. : Altered dynamics in the renal lymphatic circulation of type 1 and type 2 diabetic mice. [Internet] [Thesis]. Fukuoka Dental College / 福岡歯科大学; 2014. [cited 2020 Feb 20].
Available from: http://id.nii.ac.jp/1167/00000009/.
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
Council of Science Editors:
Uchiyama, Takanobu; Takata, Shunsuke; Ishikawa H. Altered dynamics in the renal lymphatic circulation of type 1 and type 2 diabetic mice. : Altered dynamics in the renal lymphatic circulation of type 1 and type 2 diabetic mice. [Thesis]. Fukuoka Dental College / 福岡歯科大学; 2014. Available from: http://id.nii.ac.jp/1167/00000009/
Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation
University of Alberta
21.
Desai, Prajakta V.
Deciphering the Claudins that Mediate Renal Calcium
Reabsorption.
Degree: MS, Medical Sciences-Paediatrics, 2012, University of Alberta
URL: https://era.library.ualberta.ca/files/7s75dd66w
► Kidney stones and osteoporosis are prevalent clinical conditions posing a large economic burden to the healthcare system. A common risk factor for both these diseases…
(more)
▼ Kidney stones and osteoporosis are prevalent clinical
conditions posing a large economic burden to the healthcare system.
A common risk factor for both these diseases is hypercalciuria,
which is the inappropriate excretion of calcium in urine. Changes
in serum calcium levels are detected by the calcium sensing
receptor (CaSR). In the kidney, the CaSR is localized in tubular
segments where calcium (Ca2+) flux occurs via the paracellular
pathway, specifically the proximal tubule and the thick ascending
limb of Henle’s loop (TAL). Claudins are proteins localized in the
tight junction of epithelia that control paracellular ion flux.
Recently, claudin-14 (Cldn14) expression was observed in the TAL.
We found that Cldn14 is regulated by dietary Ca2+ intake and by
elevated serum Ca2+ levels after prolonged 1,25-dihydroxyvitamin D3
administration in mice. Consistent with this, in vivo activation of
the CaSR by administration of the calcimimetic Cinacalcet, lead to
a 40-fold increase in Cldn14 mRNA abundance. Overexpression of
Cldn14 in a renal tubular cell culture model inhibited paracellular
Ca2+ flux. Together the data suggests that when serum Ca2+ level
increases it activates the CaSR leading to increased Cldn14
expression in the TAL. This in turn blocks Ca2+ reabsorption and
induces calciuria. Dysregulation of this newly described
CaSR-Cldn14 axis likely contributes to the development of
hypercalciuria and kidney stones.
Subjects/Keywords: Kidney Stones; Osteoporosis; Claudins; Calcium
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APA (6th Edition):
Desai, P. V. (2012). Deciphering the Claudins that Mediate Renal Calcium
Reabsorption. (Masters Thesis). University of Alberta. Retrieved from https://era.library.ualberta.ca/files/7s75dd66w
Chicago Manual of Style (16th Edition):
Desai, Prajakta V. “Deciphering the Claudins that Mediate Renal Calcium
Reabsorption.” 2012. Masters Thesis, University of Alberta. Accessed February 20, 2020.
https://era.library.ualberta.ca/files/7s75dd66w.
MLA Handbook (7th Edition):
Desai, Prajakta V. “Deciphering the Claudins that Mediate Renal Calcium
Reabsorption.” 2012. Web. 20 Feb 2020.
Vancouver:
Desai PV. Deciphering the Claudins that Mediate Renal Calcium
Reabsorption. [Internet] [Masters thesis]. University of Alberta; 2012. [cited 2020 Feb 20].
Available from: https://era.library.ualberta.ca/files/7s75dd66w.
Council of Science Editors:
Desai PV. Deciphering the Claudins that Mediate Renal Calcium
Reabsorption. [Masters Thesis]. University of Alberta; 2012. Available from: https://era.library.ualberta.ca/files/7s75dd66w
University of Alberta
22.
Chow, Aaron K.Y.
A novel mouse model of acute antibody-mediated transplant
rejection shows simultaneous complement activating and
anti-inflammatory effects of donor specific IgG antibodies.
Degree: MS, Medical Sciences- Laboratory Medicine and
Pathology, 2013, University of Alberta
URL: https://era.library.ualberta.ca/files/ht24wk73f
► We developed a small animal model of pure antibody-mediated rejection (ABMR) by utilizing fully major histocompatibility complex (MHC) mismatched kidney allografts in mice. By examining…
(more)
▼ We developed a small animal model of pure
antibody-mediated rejection (ABMR) by utilizing fully major
histocompatibility complex (MHC) mismatched kidney allografts in
mice. By examining resultant ABMR pathology, we show that terminal
complement products C5a and C5b-9 are crucial in establishing
antibody-mediated allograft microvascular and tubular injury, and
the presence of proximal complement product C4d alone is not
indicative of ABMR. We demonstrate allorecognition capabilities in
NK cells to induce micro-inflammation and apoptosis in MHC
mismatched transplants. By gene expression profiling, we found that
binding of donor specific antibodies (DSA) into kidney allograft
tissues induces a ‘self-protective’ anti-inflammatory response in
kidney parenchyma. The data suggests that the anti-inflammatory
response associated with DSA may be regulated via inhibitory Fc
receptors for immunoglobulin G. In summary, we established a mouse
model demonstrating pure acute ABMR of kidney allografts mimicking
some aspects of human ABMR pathology in the absence of T
cell-mediated rejection.
Subjects/Keywords: Transplantation; Antibody; Rejection; Kidney
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APA (6th Edition):
Chow, A. K. Y. (2013). A novel mouse model of acute antibody-mediated transplant
rejection shows simultaneous complement activating and
anti-inflammatory effects of donor specific IgG antibodies. (Masters Thesis). University of Alberta. Retrieved from https://era.library.ualberta.ca/files/ht24wk73f
Chicago Manual of Style (16th Edition):
Chow, Aaron K Y. “A novel mouse model of acute antibody-mediated transplant
rejection shows simultaneous complement activating and
anti-inflammatory effects of donor specific IgG antibodies.” 2013. Masters Thesis, University of Alberta. Accessed February 20, 2020.
https://era.library.ualberta.ca/files/ht24wk73f.
MLA Handbook (7th Edition):
Chow, Aaron K Y. “A novel mouse model of acute antibody-mediated transplant
rejection shows simultaneous complement activating and
anti-inflammatory effects of donor specific IgG antibodies.” 2013. Web. 20 Feb 2020.
Vancouver:
Chow AKY. A novel mouse model of acute antibody-mediated transplant
rejection shows simultaneous complement activating and
anti-inflammatory effects of donor specific IgG antibodies. [Internet] [Masters thesis]. University of Alberta; 2013. [cited 2020 Feb 20].
Available from: https://era.library.ualberta.ca/files/ht24wk73f.
Council of Science Editors:
Chow AKY. A novel mouse model of acute antibody-mediated transplant
rejection shows simultaneous complement activating and
anti-inflammatory effects of donor specific IgG antibodies. [Masters Thesis]. University of Alberta; 2013. Available from: https://era.library.ualberta.ca/files/ht24wk73f
University of Edinburgh
23.
Evans, Louise Christine.
Role of 11βHSD2 in salt and water homeostasis.
Degree: PhD, 2012, University of Edinburgh
URL: http://hdl.handle.net/1842/6531
► 11β-Hydroxysteroid Dehydrogenase Type 2 (11βHSD2) catalyses the inactivation of cortisol. In aldosterone target tissues co-expression of 11βHSD2 and mineralocorticoid receptors (MR) protects the receptor from…
(more)
▼ 11β-Hydroxysteroid Dehydrogenase Type 2 (11βHSD2) catalyses the inactivation of cortisol. In aldosterone target tissues co-expression of 11βHSD2 and mineralocorticoid receptors (MR) protects the receptor from activation by glucocorticoids. In the syndrome of Apparent Mineralocorticoid Excess, mutations in the HSD11B2 gene cause hypertension, which is thought to be driven by volume expansion secondary to sodium retention. 11βHSD2 mice are indeed hypertensive but paradoxically volume contracted, suggestive of a urine-concentrating defect. The current studies were designed to evaluate sodium and water homeostasis in 11βHSD2-/- mice. 11βHSD2-/- mice developed a severe and progressive polyuric-polydipsic phenotype. Despite basal polyuria, at <100 days 11βHSD2-/- mice had a functional concentration response when challenged with 24 hours water deprivation. At >180 days the exacerbated polyuria was associated with severe medullary injury in the null mice. Basal aquaporin 2 (AQP2) abundance was reduced in the 11βHSD2-/- mice at both <100 and >180 days. Moreover, vasopressin 2 receptor (V2R) stimulation failed to normalize the impaired response to water deprivation in >180 day null mice. Consequently, a renal origin to the polyuria was postulated. Indeed, mice in which 11βHSD2 had been selectively targeted in the brain had a normal water turnover. A key finding from these studies is that functional deletion of 11βHSD2 in the brain, specifically the nucleus of the solitary tract (NTS), resulted in an increased salt appetite. Moreover, the mice displayed a preference for 1.5% NaCl over water. Blockade of mineralocorticoid receptors (MR) significantly reduced NaCl intake. This is the first demonstration of an increased salt appetite in a model with normal renal function and in the absence of sodium depletion. These data implicate activation of MR on 11βHSD2 positive neurons in the NTS in the behavioural drive to consume sodium.
Subjects/Keywords: kidney; sodium; water; homeostasis
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APA (6th Edition):
Evans, L. C. (2012). Role of 11βHSD2 in salt and water homeostasis. (Doctoral Dissertation). University of Edinburgh. Retrieved from http://hdl.handle.net/1842/6531
Chicago Manual of Style (16th Edition):
Evans, Louise Christine. “Role of 11βHSD2 in salt and water homeostasis.” 2012. Doctoral Dissertation, University of Edinburgh. Accessed February 20, 2020.
http://hdl.handle.net/1842/6531.
MLA Handbook (7th Edition):
Evans, Louise Christine. “Role of 11βHSD2 in salt and water homeostasis.” 2012. Web. 20 Feb 2020.
Vancouver:
Evans LC. Role of 11βHSD2 in salt and water homeostasis. [Internet] [Doctoral dissertation]. University of Edinburgh; 2012. [cited 2020 Feb 20].
Available from: http://hdl.handle.net/1842/6531.
Council of Science Editors:
Evans LC. Role of 11βHSD2 in salt and water homeostasis. [Doctoral Dissertation]. University of Edinburgh; 2012. Available from: http://hdl.handle.net/1842/6531
Oregon State University
24.
Biuk, Abdulmajid Ahmed.
Physiological and yield responses of snap beans (Phaseolus vulgaris) to water availability.
Degree: PhD, Soil Science, 1982, Oregon State University
URL: http://hdl.handle.net/1957/42025
Subjects/Keywords: Kidney bean
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APA (6th Edition):
Biuk, A. A. (1982). Physiological and yield responses of snap beans (Phaseolus vulgaris) to water availability. (Doctoral Dissertation). Oregon State University. Retrieved from http://hdl.handle.net/1957/42025
Chicago Manual of Style (16th Edition):
Biuk, Abdulmajid Ahmed. “Physiological and yield responses of snap beans (Phaseolus vulgaris) to water availability.” 1982. Doctoral Dissertation, Oregon State University. Accessed February 20, 2020.
http://hdl.handle.net/1957/42025.
MLA Handbook (7th Edition):
Biuk, Abdulmajid Ahmed. “Physiological and yield responses of snap beans (Phaseolus vulgaris) to water availability.” 1982. Web. 20 Feb 2020.
Vancouver:
Biuk AA. Physiological and yield responses of snap beans (Phaseolus vulgaris) to water availability. [Internet] [Doctoral dissertation]. Oregon State University; 1982. [cited 2020 Feb 20].
Available from: http://hdl.handle.net/1957/42025.
Council of Science Editors:
Biuk AA. Physiological and yield responses of snap beans (Phaseolus vulgaris) to water availability. [Doctoral Dissertation]. Oregon State University; 1982. Available from: http://hdl.handle.net/1957/42025
Oregon State University
25.
Sader, Rubens.
Effect of N and P fertilizers on growth, nitrate reductase activity, seed production and seed quality of snap bean (Phaseolus vulgaris L.).
Degree: PhD, Crop Science, 1979, Oregon State University
URL: http://hdl.handle.net/1957/42775
Subjects/Keywords: Kidney bean
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APA (6th Edition):
Sader, R. (1979). Effect of N and P fertilizers on growth, nitrate reductase activity, seed production and seed quality of snap bean (Phaseolus vulgaris L.). (Doctoral Dissertation). Oregon State University. Retrieved from http://hdl.handle.net/1957/42775
Chicago Manual of Style (16th Edition):
Sader, Rubens. “Effect of N and P fertilizers on growth, nitrate reductase activity, seed production and seed quality of snap bean (Phaseolus vulgaris L.).” 1979. Doctoral Dissertation, Oregon State University. Accessed February 20, 2020.
http://hdl.handle.net/1957/42775.
MLA Handbook (7th Edition):
Sader, Rubens. “Effect of N and P fertilizers on growth, nitrate reductase activity, seed production and seed quality of snap bean (Phaseolus vulgaris L.).” 1979. Web. 20 Feb 2020.
Vancouver:
Sader R. Effect of N and P fertilizers on growth, nitrate reductase activity, seed production and seed quality of snap bean (Phaseolus vulgaris L.). [Internet] [Doctoral dissertation]. Oregon State University; 1979. [cited 2020 Feb 20].
Available from: http://hdl.handle.net/1957/42775.
Council of Science Editors:
Sader R. Effect of N and P fertilizers on growth, nitrate reductase activity, seed production and seed quality of snap bean (Phaseolus vulgaris L.). [Doctoral Dissertation]. Oregon State University; 1979. Available from: http://hdl.handle.net/1957/42775
Oregon State University
26.
Edwards, Cynthia Ann.
Cytokinin-active ribonucleosides in Phaseolus vulgaris L. transfer ribonucleic acids.
Degree: MA, Botany, 1978, Oregon State University
URL: http://hdl.handle.net/1957/42981
Subjects/Keywords: Kidney bean
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APA (6th Edition):
Edwards, C. A. (1978). Cytokinin-active ribonucleosides in Phaseolus vulgaris L. transfer ribonucleic acids. (Masters Thesis). Oregon State University. Retrieved from http://hdl.handle.net/1957/42981
Chicago Manual of Style (16th Edition):
Edwards, Cynthia Ann. “Cytokinin-active ribonucleosides in Phaseolus vulgaris L. transfer ribonucleic acids.” 1978. Masters Thesis, Oregon State University. Accessed February 20, 2020.
http://hdl.handle.net/1957/42981.
MLA Handbook (7th Edition):
Edwards, Cynthia Ann. “Cytokinin-active ribonucleosides in Phaseolus vulgaris L. transfer ribonucleic acids.” 1978. Web. 20 Feb 2020.
Vancouver:
Edwards CA. Cytokinin-active ribonucleosides in Phaseolus vulgaris L. transfer ribonucleic acids. [Internet] [Masters thesis]. Oregon State University; 1978. [cited 2020 Feb 20].
Available from: http://hdl.handle.net/1957/42981.
Council of Science Editors:
Edwards CA. Cytokinin-active ribonucleosides in Phaseolus vulgaris L. transfer ribonucleic acids. [Masters Thesis]. Oregon State University; 1978. Available from: http://hdl.handle.net/1957/42981
Oregon State University
27.
Filgueiras, Tarciso de Sousa.
Effect of variety, planting date and plant population on yield and quality of Snap Bean (Phaseolus vulgaris L.) seed.
Degree: MS, Agronomic Crop Science, 1977, Oregon State University
URL: http://hdl.handle.net/1957/43234
► The objectives of these experiments were to determine the feasibility of saving seed from snap bean fields in the Willamette Valley that have been bypassed…
(more)
▼ The objectives of these experiments were to determine
the feasibility of saving seed from snap bean fields in the
Willamette Valley that have been bypassed for canning and
freezing, and to study the effects of different seed production
methods on yield and quality of snap bean (Phaseolus
vulgaris L.).
Field experiments were conducted over a period of two
years at the Oregon State University Vegetable Research
Farm at Corvallis. The cultivars OSU 1604 and Asgrow 290
were used in these trials. Planting dates in 1975 were
May 17, June 20, and July 3, and in 1976 were April 29,
May 13, June 4, and June 22. Seeding rates of 165,000,
330,000 and 495,000 seeds/ha were used in both years.
The quality of the harvested seed was assessed in
terms of germination, seed size, protein content, seedling
root length, seedling dry weight, halo blight infection,
and field performance.
The data indicate that technically it is feasible to
produce snap bean seed in the Willamette Valley with yield
and quality equal to those produced in traditional seed
production areas.
It is also suggested that high quality bean seed can
be harvested from fields that have been bypassed for processing,
provided they have been planted.
The seed production methods tested affected nearly all
aspects of the crop. Early plantings provided greater
yields and delayed plantings decreased yields up to 62%.
When planting was delayed until July 3, no seed was harvested
because the crop did not mature before the fall
rains.
The three seeding rates tested did not affect total
seed yield appreciably.
Laboratory evaluations in 1975 showed no appreciable
effects of planting date and seeding rate on seed quality.
Field evaluation, however, showed that for Asgrow 290,
seeds from the second planting had better stands and produced
20% more pods than seeds from the first planting.
Likewise, seeds grown at low density produced 10% and 23%
more pods than seeds grown at medium and high densities,
respectively. OSU 1604 was not affected by cultural
practices.
In 1976, yield and seed quality of both cultivars were
affected by planting date and seeding rate in the following
ways: early plantings and low seeding rates produced
greater yields, larger and heavier seeds and heavier
seedlings. Late plantings and high seeding rates depressed
yields, seed size and seedling weight. Protein content of
seed was not affected by either of these variables.
Advisors/Committee Members: Grabe, Don F. (advisor).
Subjects/Keywords: Kidney bean
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APA (6th Edition):
Filgueiras, T. d. S. (1977). Effect of variety, planting date and plant population on yield and quality of Snap Bean (Phaseolus vulgaris L.) seed. (Masters Thesis). Oregon State University. Retrieved from http://hdl.handle.net/1957/43234
Chicago Manual of Style (16th Edition):
Filgueiras, Tarciso de Sousa. “Effect of variety, planting date and plant population on yield and quality of Snap Bean (Phaseolus vulgaris L.) seed.” 1977. Masters Thesis, Oregon State University. Accessed February 20, 2020.
http://hdl.handle.net/1957/43234.
MLA Handbook (7th Edition):
Filgueiras, Tarciso de Sousa. “Effect of variety, planting date and plant population on yield and quality of Snap Bean (Phaseolus vulgaris L.) seed.” 1977. Web. 20 Feb 2020.
Vancouver:
Filgueiras TdS. Effect of variety, planting date and plant population on yield and quality of Snap Bean (Phaseolus vulgaris L.) seed. [Internet] [Masters thesis]. Oregon State University; 1977. [cited 2020 Feb 20].
Available from: http://hdl.handle.net/1957/43234.
Council of Science Editors:
Filgueiras TdS. Effect of variety, planting date and plant population on yield and quality of Snap Bean (Phaseolus vulgaris L.) seed. [Masters Thesis]. Oregon State University; 1977. Available from: http://hdl.handle.net/1957/43234
Oregon State University
28.
Detongnon, Jean.
Inheritance of stem elongation tendency in determinate forms of Phaseolus vulgaris (L.).
Degree: PhD, Horticulture, 1984, Oregon State University
URL: http://hdl.handle.net/1957/25541
Subjects/Keywords: Kidney bean
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APA (6th Edition):
Detongnon, J. (1984). Inheritance of stem elongation tendency in determinate forms of Phaseolus vulgaris (L.). (Doctoral Dissertation). Oregon State University. Retrieved from http://hdl.handle.net/1957/25541
Chicago Manual of Style (16th Edition):
Detongnon, Jean. “Inheritance of stem elongation tendency in determinate forms of Phaseolus vulgaris (L.).” 1984. Doctoral Dissertation, Oregon State University. Accessed February 20, 2020.
http://hdl.handle.net/1957/25541.
MLA Handbook (7th Edition):
Detongnon, Jean. “Inheritance of stem elongation tendency in determinate forms of Phaseolus vulgaris (L.).” 1984. Web. 20 Feb 2020.
Vancouver:
Detongnon J. Inheritance of stem elongation tendency in determinate forms of Phaseolus vulgaris (L.). [Internet] [Doctoral dissertation]. Oregon State University; 1984. [cited 2020 Feb 20].
Available from: http://hdl.handle.net/1957/25541.
Council of Science Editors:
Detongnon J. Inheritance of stem elongation tendency in determinate forms of Phaseolus vulgaris (L.). [Doctoral Dissertation]. Oregon State University; 1984. Available from: http://hdl.handle.net/1957/25541
University of Hawaii – Manoa
29.
Tsao, Shing-jy J.
The inheritance of photoperiodism in snap bean (Phaseolus vulgaris).
Degree: PhD, 2009, University of Hawaii – Manoa
URL: http://hdl.handle.net/10125/9896
► Typescript.
Bibliography: leaves [62]-67.
Microfiche.
viii, 67 leaves ill
The heredity of photoperiodic response of flowering in Phaseo1us vulgaris was studied. The parents were classified…
(more)
▼ Typescript.
Bibliography: leaves [62]-67.
Microfiche.
viii, 67 leaves ill
The heredity of photoperiodic response of flowering in Phaseo1us vulgaris was studied. The parents were classified into three types according to their photoperiod sensitivity – day-neutra1 (flower at any day1ength), intermediate (require a night longer than 11.5 hours), and sensitive (require a night longer than 12 hours). Crosses between parents of the same phenotype generally produced F1 and F2 progenies which showed no segregation. The segregation patterns for photoperiodic response were determined for larger numbers of individuals by planting during the summer when days are too long for floral induction and assuming that each plant begins to flower when the daylength has shortened to the critical length required by that plant. Temperatures within the range experienced in the field were found to have an insignificant effect. It is postulated that the inheritance of the photoperiodic response in these lines is determined by at least four major gene loci with dominance, epistasis, and independent segregation. A dominant N gene is postulated that permits flowering at any day1ength. If the recessive n gene or a dominant inhibitor of the N gene, IN, are present, there is an intermediate day1ength requirement for flowering. A dominant Q gene which intensifies the short day length requirement is also postulated. If the recessive q gene or a dominant inhibitor of the Q gene, IQ, are present, the day1ength requirement again is of the intermediate type. The day-neutral and intermediate parents therefore differ by two genes (at the N and IN loci), and the intermediate and sensitive parents differ by another two genes (at the Q and IQ loci), so that the day-neutral and sensitive parents differ by a total of four genes. It is likely that additional genes with smaller effects may also be involved.
Subjects/Keywords: Photoperiodism; Kidney bean – Hawaii – Oahu
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APA (6th Edition):
Tsao, S. J. (2009). The inheritance of photoperiodism in snap bean (Phaseolus vulgaris). (Doctoral Dissertation). University of Hawaii – Manoa. Retrieved from http://hdl.handle.net/10125/9896
Chicago Manual of Style (16th Edition):
Tsao, Shing-jy J. “The inheritance of photoperiodism in snap bean (Phaseolus vulgaris).” 2009. Doctoral Dissertation, University of Hawaii – Manoa. Accessed February 20, 2020.
http://hdl.handle.net/10125/9896.
MLA Handbook (7th Edition):
Tsao, Shing-jy J. “The inheritance of photoperiodism in snap bean (Phaseolus vulgaris).” 2009. Web. 20 Feb 2020.
Vancouver:
Tsao SJ. The inheritance of photoperiodism in snap bean (Phaseolus vulgaris). [Internet] [Doctoral dissertation]. University of Hawaii – Manoa; 2009. [cited 2020 Feb 20].
Available from: http://hdl.handle.net/10125/9896.
Council of Science Editors:
Tsao SJ. The inheritance of photoperiodism in snap bean (Phaseolus vulgaris). [Doctoral Dissertation]. University of Hawaii – Manoa; 2009. Available from: http://hdl.handle.net/10125/9896
University of Edinburgh
30.
O'Neill, Stephen.
Role of heat shock protein 90 in modulating ischemia-reperfusion injury in the kidney.
Degree: PhD, 2015, University of Edinburgh
URL: http://hdl.handle.net/1842/20410
► Kidney transplantation is the gold standard treatment for end-stage renal disease. Renal ischemia-reperfusion injury is an unavoidable consequence of the transplantation procedure and is responsible…
(more)
▼ Kidney transplantation is the gold standard treatment for end-stage renal disease. Renal ischemia-reperfusion injury is an unavoidable consequence of the transplantation procedure and is responsible for delayed graft function and poorer long-term outcomes. Pharmacological inhibition of heat shock protein 90 is a preconditioning strategy that has previously been shown to reduce renal ischemia-reperfusion injury. However, the clinical application of heat shock protein 90 inhibitors is limited by their toxicity profile and the exact mechanisms of protection conferred are unknown. The aims of this thesis were to establish mechanisms of protection offered by these drugs and investigate a less toxic analogue that has the potential to be safely translated into human studies. AT13387 is a novel small molecule heat shock protein 90 inhibitor with a low toxicity profile, which is being evaluated in phase II studies in oncology and therefore has excellent translational potential in the context of transplantation. Heat shock protein 90 inhibition up-regulates protective heat shock proteins (especially heat shock protein 70) and potentially down-regulates NF-ҡB activity by disruption of the IҡB kinase complex. Toll-like receptor 4 is a further regulator of NF-ҡB activity and studies have suggested that Toll-like receptor 4 plays a dominant role in mediating kidney damage following ischemia-reperfusion injury. To explore potential molecular mechanisms of protection, human embryonic kidney cells were pre-treated with AT13387 and exposed to endotoxin-free hyaluronan to stimulate sterile Toll-like receptor 4-specific NF-ҡB activation. AT13387-treatment resulted in breakdown of IҡB kinase, which abolished Toll-like receptor 4-mediated NF-ҡB activation by hyaluronan. Inhibition of autophagy prevented IҡB kinase-α degradation by heat shock protein 90 inhibition and resulted in regain of NF-ҡB activity by hyaluronan. In subsequent investigations, AT13387 decreased pro-inflammatory cytokine release following hyaluronan stimulation and increased cell viability in an in vitro model of oxidative stress. In mice, AT13387 induced heat shock protein 70 expression in the kidney. AT13387 pre-treatment then significantly reduced kidney injury following renal ischemia-reperfusion injury. In contrast, in severe combined immunodeficient mice, AT13387 no longer reduced kidney injury from renal ischemia-reperfusion injury. This emphasises the potential importance of the adaptive immune system in the protective effect of this agent. This resonates with reports of heat shock protein 70 up-regulation in the context of heat preconditioning, which leads to renal protection from renal ischemia-reperfusion injury that is lymphocyte-dependent. Secondary lung injury is an additional consequence of renal ischemia-reperfusion injury. In further experiments, pre-treatment with AT13387 again did not reduce kidney injury following renal ischemia-reperfusion injury in severe combined immunodeficient mice. However, AT13387 did reduce secondary lung injury. This lung…
Subjects/Keywords: 617.4; kidney; ischemia; transplant
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APA (6th Edition):
O'Neill, S. (2015). Role of heat shock protein 90 in modulating ischemia-reperfusion injury in the kidney. (Doctoral Dissertation). University of Edinburgh. Retrieved from http://hdl.handle.net/1842/20410
Chicago Manual of Style (16th Edition):
O'Neill, Stephen. “Role of heat shock protein 90 in modulating ischemia-reperfusion injury in the kidney.” 2015. Doctoral Dissertation, University of Edinburgh. Accessed February 20, 2020.
http://hdl.handle.net/1842/20410.
MLA Handbook (7th Edition):
O'Neill, Stephen. “Role of heat shock protein 90 in modulating ischemia-reperfusion injury in the kidney.” 2015. Web. 20 Feb 2020.
Vancouver:
O'Neill S. Role of heat shock protein 90 in modulating ischemia-reperfusion injury in the kidney. [Internet] [Doctoral dissertation]. University of Edinburgh; 2015. [cited 2020 Feb 20].
Available from: http://hdl.handle.net/1842/20410.
Council of Science Editors:
O'Neill S. Role of heat shock protein 90 in modulating ischemia-reperfusion injury in the kidney. [Doctoral Dissertation]. University of Edinburgh; 2015. Available from: http://hdl.handle.net/1842/20410
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