Advanced search options

Advanced Search Options 🞨

Browse by author name (“Author name starts with…”).

Find ETDs with:

in
/  
in
/  
in
/  
in

Written in Published in Earliest date Latest date

Sorted by

Results per page:

You searched for subject:(kbiol). One record found.

Search Limiters

Last 2 Years | English Only

No search limiters apply to these results.

▼ Search Limiters


Université du Luxembourg

1. Köhler, Christian. Biopharm - the Influence of Macro-substrates & Conditioning on Pharmaceutical Removal Rates by Moving Bed Biofilm Reactors.

Degree: 2015, Université du Luxembourg

Organic micropollutants with endocrine disruptive properties are present in the aquatic environment. A major part of their emission is caused by municipal wastewater treatment plants (WWTPs). For this reason, a vast amount of research has been undertaken to remove xenobiotics from municipal wastewater by developing post-treatment technologies with some success. However, these technologies cause considerable environmental costs due their high demand for electrical energy implicating an increase in CO2 emissions. Consequently, existing biological treatments need first to be better understood and subsequently optimized regarding xenobiotic removal before post-treatments are employed. The study focused on the fate of xenobiotics during biological wastewater treatment. In particular, metabolic strategies of bacteria degrading pharmaceuticals were investigated within moving bed biofilm reactor (MBBR) processes. Two main objectives were tracked. On the one hand, it was to unfold the impact of macro-substrates in terms of type and molecular complexity on the activity of microorganisms and consequently pharmaceutical degradation performance. On the other hand, the study was set out to explore the adaptation of metabolic means regarding exoenzymes and consortia structure during continuous (long-term) exposure to pharmaceuticals. Accordingly, the ability to increase microbial competences during pharmaceutical short-term pulses was the general target of investigation. Both conditions continuous substance flow and short-term peak loads of xenobiotics are believed to occur in urban WWTPs. A pilot MBBR was set up next to a domestic WWTP. The pilot treated municipal sewage and served as inoculation reservoir for biofilm carriers used for in-depth laboratory experiments. The latter comprised six lab-scale MBBRs featuring flow through operation under controlled conditions regarding temperature, dissolved oxygen, pH, influent flow and influent load. The reactors were conditioned over four weeks with a synthetic sewage providing substrates and micro-nutrients in a similar manner as expected under real conditions. Biofilm was monitored by respirometry and a series of enzyme assays using fluorogenic substrates to capture esterase, phosphatase, alpha- and beta-glucosidase and aminopeptidase activity. All enzymes are essential during organic carbon metabolism. An array of macro-substrates with different molecular complexity was triggering individual enzyme activity profiles. After conditioning, 12 pharmaceuticals being subject to a range of anticipated metabolic pathways and degradation rates were spiked into the MBBRs. Their degradation kinetics were measured by liquid chromatography coupled with tandem mass spectrometry (LC MS/MS). Pseudo first-order kinetics revealed substrate related fingerprints and showed that readily biodegradable substrate leads generally to good pharmaceutical degradation performance compared to synthetic sewage with a mixture of several high molecular organic substrates. The latter was designed to induce the… Advisors/Committee Members: Hansen, Joachim [superviser], Wilmes, Paul [superviser], Vanrolleghem, Peter [superviser], Schosseler, Paul [superviser], Galle, Tom [superviser], Sauter, Thomas [president of the jury].

Subjects/Keywords: pharmaceuticals; xenobiotics; wastewater; wwtp; moving bed biofilm reactor; MBBR; wastewater treatment plant; lab-scale; metabolism; co-metabolism; training; conditioning; 16S rRNA; DNA; RNA; cDNA; enzyme; fingerprinting; consortia; structure; enzyme activity; respirometry; maximum growth rate; SOUR; degradation kinetics; kbiol; macro-substrate; substrate; LC MS/MS; esterase; phosphatase; glucosidase; aminopeptidase; exoenzyme; activity; atenolol; diclofenac; PCA; PCoA; Delftia; Lysobacter; Life sciences :: Biochemistry, biophysics & molecular biology [F05]; Sciences du vivant :: Biochimie, biophysique & biologie moléculaire [F05]; Life sciences :: Biotechnology [F06]; Sciences du vivant :: Biotechnologie [F06]; Life sciences :: Environmental sciences & ecology [F08]; Sciences du vivant :: Sciences de l'environnement & écologie [F08]; Engineering, computing & technology :: Civil engineering [C04]; Ingénierie, informatique & technologie :: Ingénierie civile [C04]; Engineering, computing & technology :: Multidisciplinary, general & others [C99]; Ingénierie, informatique & technologie :: Multidisciplinaire, généralités & autres [C99]

Record DetailsSimilar RecordsGoogle PlusoneFacebookTwitterCiteULikeMendeleyreddit

APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6th Edition):

Köhler, C. (2015). Biopharm - the Influence of Macro-substrates & Conditioning on Pharmaceutical Removal Rates by Moving Bed Biofilm Reactors. (Doctoral Dissertation). Université du Luxembourg. Retrieved from http://orbilu.uni.lu/handle/10993/22418

Chicago Manual of Style (16th Edition):

Köhler, Christian. “Biopharm - the Influence of Macro-substrates & Conditioning on Pharmaceutical Removal Rates by Moving Bed Biofilm Reactors.” 2015. Doctoral Dissertation, Université du Luxembourg. Accessed September 26, 2020. http://orbilu.uni.lu/handle/10993/22418.

MLA Handbook (7th Edition):

Köhler, Christian. “Biopharm - the Influence of Macro-substrates & Conditioning on Pharmaceutical Removal Rates by Moving Bed Biofilm Reactors.” 2015. Web. 26 Sep 2020.

Vancouver:

Köhler C. Biopharm - the Influence of Macro-substrates & Conditioning on Pharmaceutical Removal Rates by Moving Bed Biofilm Reactors. [Internet] [Doctoral dissertation]. Université du Luxembourg; 2015. [cited 2020 Sep 26]. Available from: http://orbilu.uni.lu/handle/10993/22418.

Council of Science Editors:

Köhler C. Biopharm - the Influence of Macro-substrates & Conditioning on Pharmaceutical Removal Rates by Moving Bed Biofilm Reactors. [Doctoral Dissertation]. Université du Luxembourg; 2015. Available from: http://orbilu.uni.lu/handle/10993/22418

.