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You searched for subject:(izolovani citotrofoblast). Showing records 1 – 2 of 2 total matches.

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1. Kolundžić Nikola. Galectin-1 and functional properties of human trophoblast cells in vitro.

Degree: PhD, Biology, 2012, University of Belgrade

Galectin-1 (gal-1) is expressed at the fetomaternal interface in a manner suggestive of a potential involvement in cell adhesion, migration and invasion of the human trophoblast. These processes are considered essential for the embryo implantation and formation of the functional placenta. However, no studies have examined the role of the gal-1 in the human placenta. Hypothesis that gal-1 may be relevant for trophoblast invasion was addressed by this thesis for the first time. To this end appropriate functional tests of cell adhesion, migration and invasion were performed in vitro, using invasive trophoblast models (isolated first trimester cytotrophoblast, immortalized extravillous HTR-8/SVneo cell line and choriocarcinoma JAr cell line). Since gal-1 is synthesised by both extravillous cytotrophoblast and decidual stromal cells in vivo, selected approaches addressed possible effect of both endogenous and exogenous gal-1. Use of function blocking anti-gal-1 antibody provided an insight into participation of endogenous gal-1, while supplementation by either molecular form of biologically active recombinant human gal-1, CS-gal-1 (with lectin activity) or Ox-gal-1 (without lectin activity) indicated relevance of exogenous gal-1 for trophoblast invasion in vitro. The importance of lectin-type interactions was investigated primarily using lactose (an inhibitory sugar for galectins). Since the expression patterns and functions of galectins often overlap, galectin profile of the invasive trophoblast at RNA and protein levels was established here. This is the first study to address the functional relevance of any galectin for human trophoblast invasivness in vitro, qualifying gal-1 as an important member of the trophoblast cell invasion machinery. It is documented here that endogenous gal-1, as well as additional exogenous gal-1 (both forms, but particularly the form with lectin activity) participate in trophoblast invasion, suggesting possible involvement of trophoblastic and decidual gal-1 in this process in vivo. Galectin profile of the invasive trophoblast was established here for the first time, which comprised of gal-1, gal-3 and gal-8. The obtained findings improve current understanding of the biological relevance of the gal-1 for the trophoblast function in vitro. These results provide the basis for further investigation of the mechanisms and effector molecules involved in gal-1 induced processes. Potential relevance of other expressed members of the galectin family for the invasive trophoblast remains to be elucidated.

U zoni interakcije majke i fetusa galektin-1 (gal-1) je eksprimiran na način koji ukazuje na njegovu moguću ulogu u ćelijskoj adheziji, migraciji i invaziji trofoblasta, procesima koji su ključni za implantaciju embriona i formiranje funkcionalne placente. Međutim, uloga gal-1 u trofoblastu čoveka dosada nije bila ispitivana, što je predmet istraživanja ovog doktorskog rada. U tu svrhu korišćeni su odgovarajući funkcionalni in vitro testovi adhezije,…

Subjects/Keywords: gal-1; trophoblast; cell invasion; isolated cytotrophoblast; HTR-8/SVneo; JAr; galectin profile; gal-8; gal-1; trofoblast; ćelijska invazija; izolovani citotrofoblast; HTR-8/SVneo; JAr; galektinski profil; gal-8

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APA (6th Edition):

Nikola, K. (2012). Galectin-1 and functional properties of human trophoblast cells in vitro. (Doctoral Dissertation). University of Belgrade. Retrieved from http://dx.doi.org/10.2298/BG20121226KOLUNDZIC ; http://eteze.bg.ac.rs/application/showtheses?thesesId=154 ; https://fedorabg.bg.ac.rs/fedora/get/o:5390/bdef:Content/get ; http://vbs.rs/scripts/cobiss?command=SEARCH&base=99999&select=ID=1024554930

Chicago Manual of Style (16th Edition):

Nikola, Kolundžić. “Galectin-1 and functional properties of human trophoblast cells in vitro.” 2012. Doctoral Dissertation, University of Belgrade. Accessed September 15, 2019. http://dx.doi.org/10.2298/BG20121226KOLUNDZIC ; http://eteze.bg.ac.rs/application/showtheses?thesesId=154 ; https://fedorabg.bg.ac.rs/fedora/get/o:5390/bdef:Content/get ; http://vbs.rs/scripts/cobiss?command=SEARCH&base=99999&select=ID=1024554930.

MLA Handbook (7th Edition):

Nikola, Kolundžić. “Galectin-1 and functional properties of human trophoblast cells in vitro.” 2012. Web. 15 Sep 2019.

Vancouver:

Nikola K. Galectin-1 and functional properties of human trophoblast cells in vitro. [Internet] [Doctoral dissertation]. University of Belgrade; 2012. [cited 2019 Sep 15]. Available from: http://dx.doi.org/10.2298/BG20121226KOLUNDZIC ; http://eteze.bg.ac.rs/application/showtheses?thesesId=154 ; https://fedorabg.bg.ac.rs/fedora/get/o:5390/bdef:Content/get ; http://vbs.rs/scripts/cobiss?command=SEARCH&base=99999&select=ID=1024554930.

Council of Science Editors:

Nikola K. Galectin-1 and functional properties of human trophoblast cells in vitro. [Doctoral Dissertation]. University of Belgrade; 2012. Available from: http://dx.doi.org/10.2298/BG20121226KOLUNDZIC ; http://eteze.bg.ac.rs/application/showtheses?thesesId=154 ; https://fedorabg.bg.ac.rs/fedora/get/o:5390/bdef:Content/get ; http://vbs.rs/scripts/cobiss?command=SEARCH&base=99999&select=ID=1024554930

2. Kovačević Tamara. The effect of immunoglobulins with specificity significant for antiphospholipid syndrome on expression of characteristic proteins and invasiveness of human extravillous trophoblast in vitro.

Degree: PhD, Biology, 2013, University of Belgrade

Antiphospholipid antibody syndrome (APS) is an autoimmune multisystemic disorder clinically characterized by recurrent thrombosis and pregnancy morbidity, and presence of antiphospholipid antibodies (aPL) in sera of affected individuals. Presence of aPL is the most common acquired risk factor for pregnancy loss and pregnancyrelated complications. Main antigen in APS is β2-glycoprotein I (β2GPI), an evolutionarily conserved protein, which shows similarity in sequence and conformation with a number of microbial proteins. Until recently, thrombosis and inflammation were thought to be the main aPL related mechanisms leading to complications in pregnancy. More recent findings, however, indicate that aPL can exert direct effects of on placentation. This research was conducted to investigate the effects of immunoglobulins with specificity significant for APS on human extravillous trophoblast in vitro. The study included polyclonal aPL derived from APS patients (aPL+ IgG) and monoclonal antibody 26 (MAb 26) raised to tetanus toxoid with additional β2GPI specificity, originating from conformational similarity between tetanus toxoid and β2GPI. Effects of immunoglobulins with specificity important for APS on human extravillous trophoblast invasion in vitro were investigated using Matrigel invasion assay. The test showed that both aPL+ IgG and MAb 26 induce a decrease in trophoblast invasiveness, which did not result from a change in cell viability, proliferation, or apoptosis. Both antibodies, however, did affect the expression of characteristic proteins that are known to act as mediators of cell invasion. The aPL+ IgG induced decrease in trophoblast invasiveness was accompanied by reduction in matrix metalloproteinase (MMP)-9, integrin subunits α1, α5 and β1, and in levels of secreted galectin-1. Similarly, the inhibitory effect of MAb 26 on trophoblast invasion was accompanied by a decrease in MMP-2 and -9, integrin subunit α1, and galectin-1 expression. Immunocytochemistry revealed binding of both aPL+ IgG and MAb 26 to extravillous trophoblast cells. The observed binding was persistent in the absence of exogenous β2GPI and other factors derived from fetal calf serum used for cell culture. That implies expression of endogenous β2GPI, or putative receptor(s) that can bind aPL directly. Internalization of aPL in to various cell types (endothelial cells, macrofages, syncitiotrophoblast) has been reported, and represents a novel mechanism of aPL induced effects. Analysis of both types of trophoblast cells following incubation with aPL+ IgG and MAb 26 revealed intracellular localization of the antibodies in some of the cells. Number of cells with intracellular localization of the antibodies, as well as the staining intensity, positively correlated with the duration of incubation. MAb 26 internalization into HTR-8/SVneo cell was active and temperature dependent process indicating involvement of endocytosis. The absence of significant internalization of immunoglobulins used as control (aPL- IgG or mIgG) implies that the process was…

Subjects/Keywords: antiphospholipid antibodies; anti-β2-glycoprotein I antibodies; β2-glycoprotein I; antiphospholipid syndrome; placenta; molecular mimicry; isolated cytotrophoblast; HTR-8/SVneo; cell invasion; internalization; antifosfolipidna antitela; anti-β2-glikoprotein I antitela; β2-glikoprotein I; antifosfolipidni sindrom; placenta; molekulska mimikrija; izolovani citotrofoblast; HTR-8/SVneo; ćelijska invazija; internalizacija

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6th Edition):

Tamara, K. (2013). The effect of immunoglobulins with specificity significant for antiphospholipid syndrome on expression of characteristic proteins and invasiveness of human extravillous trophoblast in vitro. (Doctoral Dissertation). University of Belgrade. Retrieved from http://dx.doi.org/10.2298/BG20130628KOVACEVIC ; http://eteze.bg.ac.rs/application/showtheses?thesesId=785 ; https://fedorabg.bg.ac.rs/fedora/get/o:7102/bdef:Content/get ; http://vbs.rs/scripts/cobiss?command=SEARCH&base=99999&select=ID=1024579762

Chicago Manual of Style (16th Edition):

Tamara, Kovačević. “The effect of immunoglobulins with specificity significant for antiphospholipid syndrome on expression of characteristic proteins and invasiveness of human extravillous trophoblast in vitro.” 2013. Doctoral Dissertation, University of Belgrade. Accessed September 15, 2019. http://dx.doi.org/10.2298/BG20130628KOVACEVIC ; http://eteze.bg.ac.rs/application/showtheses?thesesId=785 ; https://fedorabg.bg.ac.rs/fedora/get/o:7102/bdef:Content/get ; http://vbs.rs/scripts/cobiss?command=SEARCH&base=99999&select=ID=1024579762.

MLA Handbook (7th Edition):

Tamara, Kovačević. “The effect of immunoglobulins with specificity significant for antiphospholipid syndrome on expression of characteristic proteins and invasiveness of human extravillous trophoblast in vitro.” 2013. Web. 15 Sep 2019.

Vancouver:

Tamara K. The effect of immunoglobulins with specificity significant for antiphospholipid syndrome on expression of characteristic proteins and invasiveness of human extravillous trophoblast in vitro. [Internet] [Doctoral dissertation]. University of Belgrade; 2013. [cited 2019 Sep 15]. Available from: http://dx.doi.org/10.2298/BG20130628KOVACEVIC ; http://eteze.bg.ac.rs/application/showtheses?thesesId=785 ; https://fedorabg.bg.ac.rs/fedora/get/o:7102/bdef:Content/get ; http://vbs.rs/scripts/cobiss?command=SEARCH&base=99999&select=ID=1024579762.

Council of Science Editors:

Tamara K. The effect of immunoglobulins with specificity significant for antiphospholipid syndrome on expression of characteristic proteins and invasiveness of human extravillous trophoblast in vitro. [Doctoral Dissertation]. University of Belgrade; 2013. Available from: http://dx.doi.org/10.2298/BG20130628KOVACEVIC ; http://eteze.bg.ac.rs/application/showtheses?thesesId=785 ; https://fedorabg.bg.ac.rs/fedora/get/o:7102/bdef:Content/get ; http://vbs.rs/scripts/cobiss?command=SEARCH&base=99999&select=ID=1024579762

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