subject:(irreversible electroporation)

Virginia Tech

1. Sano, Michael B. Theoretical Considerations of Biological Systems in the Presence of High Frequency Electric Fields: Microfluidic and Tissue Level Implications.

Degree: PhD, Biomedical Engineering, 2012, Virginia Tech

URL: http://hdl.handle.net/10919/77122

► The research presented in this dissertation is the result of our laboratory's effort to develop a microfluidic platform to interrogate, manipulate, isolate, and enrich rare… (more)

▼ The research presented in this dissertation is the result of our laboratory's effort to develop a microfluidic platform to interrogate, manipulate, isolate, and enrich rare mammalian cells dispersed within heterogeneous populations. Relevant examples of these target cells are stem cells within a differentiated population, circulating tumor cells (CTCs) in the blood stream, and tumor initiating cells (TICs) in a population of benign cancer cells. The ability to isolate any of these rare cells types with high efficiency will directly lead to advances in tissue engineering, cancer detection, and individualized medicine. This work lead directly to the development of a new cell manipulation technique, termed contactless dielectrophoresis (cDEP). In this technique, cells are isolated from direct contact with metal electrodes by employing fluid electrode channels filled with a highly conductive media. Thin insulating barriers, approximately 20 μm, serve to isolate the fluid electrode channels from the low conductivity sample buffer. The insulating barriers in a fluid-electrical system create a number of unique and interesting challenges from an electrical engineering standpoint. Primarily, they block the flow of DC currents and create a non-constant frequency response which can confound experimental results attempting to characterize the electrical characteristics of cells. Due to these, and other, considerations, the use of high-voltage high-frequency signals are necessary to successfully manipulate cells. The effect of these high frequency fields on cells are studied and applied to microfluidic and tissue level applications. In later chapters, theoretical and experimental results show how high frequency and pulsed electric fields can ablate cells and tissue. Understanding the parameters necessary to electroporate cells aids in the development of cDEP devices where this phenomenon is undesirable and gives insight towards the development of new cancer ablation therapies where targeted cell death is sought after. This work shows that there exists a finite frequency spectrum over which cDEP devices can operate in which cells are minimally affected by the induced electric fields. Finally, lessons learned in the course of the development of cDEP were adapted and applied towards cancer ablation therapies where electroporation are favorable. It was found that bursts of high frequency pulsed electric fields can successfully kill cells and ablate tissue volumes through a process termed High Frequency Irreversible Electroporation (H-FIRE). This technique is advantageous as these waveforms mitigate or eliminate muscle contractions associated with traditional IRE technologies. Similarly, the use of fluid electrodes in cDEP inspired the use of an organs vascular system as the conductive pathway to deliver pulses. This novel approach allows for the ablation of large volumes of tissue without the use of puncturing electrodes. These techniques are currently undergoing evaluation in tissue engineering applications and pre-clinical… Advisors/Committee Members: Davalos, Rafael V. (committeechair), Lu, Chang-Tien (committee member), Robertson, John L. (committee member), Rossmeisl, John H. Jr. (committee member), Brown, Gary S. (committee member), Soker, Shay (committee member).

Subjects/Keywords: Irreversible Electroporation; Contactless Dielectrophoresis

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APA (6^th Edition):

Sano, M. B. (2012). Theoretical Considerations of Biological Systems in the Presence of High Frequency Electric Fields: Microfluidic and Tissue Level Implications. (Doctoral Dissertation). Virginia Tech. Retrieved from http://hdl.handle.net/10919/77122

Chicago Manual of Style (16^th Edition):

Sano, Michael B. “Theoretical Considerations of Biological Systems in the Presence of High Frequency Electric Fields: Microfluidic and Tissue Level Implications.” 2012. Doctoral Dissertation, Virginia Tech. Accessed January 15, 2021. http://hdl.handle.net/10919/77122.

MLA Handbook (7^th Edition):

Sano, Michael B. “Theoretical Considerations of Biological Systems in the Presence of High Frequency Electric Fields: Microfluidic and Tissue Level Implications.” 2012. Web. 15 Jan 2021.

Vancouver:

Sano MB. Theoretical Considerations of Biological Systems in the Presence of High Frequency Electric Fields: Microfluidic and Tissue Level Implications. [Internet] [Doctoral dissertation]. Virginia Tech; 2012. [cited 2021 Jan 15]. Available from: http://hdl.handle.net/10919/77122.

Council of Science Editors:

Sano MB. Theoretical Considerations of Biological Systems in the Presence of High Frequency Electric Fields: Microfluidic and Tissue Level Implications. [Doctoral Dissertation]. Virginia Tech; 2012. Available from: http://hdl.handle.net/10919/77122

University of Minnesota

2. Liu, Feng. The Role of Protein Change (Cellular Protein Loss and Denaturation) in Determining Outcomes of Heating, Cryotherapy and Irreversible Electroporation.

Degree: M.S.M.E., Mechanical Engineering, 2018, University of Minnesota

URL: http://hdl.handle.net/11299/201723

► Atrial fibrillation currently affects millions of people in the US alone. Focal therapy is an increasingly attractive treatment for atrial fibrillation that avoids the debilitating… (more)

Subjects/Keywords: Atrial fibrillation; Cryoablation; Irreversible Electroporation; Radiofrequency Ablation

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APA (6^th Edition):

Liu, F. (2018). The Role of Protein Change (Cellular Protein Loss and Denaturation) in Determining Outcomes of Heating, Cryotherapy and Irreversible Electroporation. (Masters Thesis). University of Minnesota. Retrieved from http://hdl.handle.net/11299/201723

Chicago Manual of Style (16^th Edition):

Liu, Feng. “The Role of Protein Change (Cellular Protein Loss and Denaturation) in Determining Outcomes of Heating, Cryotherapy and Irreversible Electroporation.” 2018. Masters Thesis, University of Minnesota. Accessed January 15, 2021. http://hdl.handle.net/11299/201723.

MLA Handbook (7^th Edition):

Liu, Feng. “The Role of Protein Change (Cellular Protein Loss and Denaturation) in Determining Outcomes of Heating, Cryotherapy and Irreversible Electroporation.” 2018. Web. 15 Jan 2021.

Vancouver:

Liu F. The Role of Protein Change (Cellular Protein Loss and Denaturation) in Determining Outcomes of Heating, Cryotherapy and Irreversible Electroporation. [Internet] [Masters thesis]. University of Minnesota; 2018. [cited 2021 Jan 15]. Available from: http://hdl.handle.net/11299/201723.

Council of Science Editors:

Liu F. The Role of Protein Change (Cellular Protein Loss and Denaturation) in Determining Outcomes of Heating, Cryotherapy and Irreversible Electroporation. [Masters Thesis]. University of Minnesota; 2018. Available from: http://hdl.handle.net/11299/201723

Virginia Tech

3. White, Natalie B. A Patient-specific Irreversible Electroporation Treatment Planning Model Based on Human Tissue Properties.

Degree: MS, Electrical Engineering, 2018, Virginia Tech

URL: http://hdl.handle.net/10919/83535

► Pancreatic cancer results in 40,000 deaths every year in the U.S, making it one of the most challenging diseases to treat. The current treatments for… (more)

Subjects/Keywords: Irreversible electroporation; cancer treatment; tissue properties

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

White, N. B. (2018). A Patient-specific Irreversible Electroporation Treatment Planning Model Based on Human Tissue Properties. (Masters Thesis). Virginia Tech. Retrieved from http://hdl.handle.net/10919/83535

Chicago Manual of Style (16^th Edition):

White, Natalie B. “A Patient-specific Irreversible Electroporation Treatment Planning Model Based on Human Tissue Properties.” 2018. Masters Thesis, Virginia Tech. Accessed January 15, 2021. http://hdl.handle.net/10919/83535.

MLA Handbook (7^th Edition):

White, Natalie B. “A Patient-specific Irreversible Electroporation Treatment Planning Model Based on Human Tissue Properties.” 2018. Web. 15 Jan 2021.

Vancouver:

White NB. A Patient-specific Irreversible Electroporation Treatment Planning Model Based on Human Tissue Properties. [Internet] [Masters thesis]. Virginia Tech; 2018. [cited 2021 Jan 15]. Available from: http://hdl.handle.net/10919/83535.

Council of Science Editors:

White NB. A Patient-specific Irreversible Electroporation Treatment Planning Model Based on Human Tissue Properties. [Masters Thesis]. Virginia Tech; 2018. Available from: http://hdl.handle.net/10919/83535

Universitat Pompeu Fabra

4. Sánchez Velázquez, Patricia, 1985-. Effect of the irreversible electroporation in a murine model of colorectal liver metastases.

Degree: Departament de Ciències Experimentals i de la Salut, 2017, Universitat Pompeu Fabra

URL: http://hdl.handle.net/10803/565772

► La percepció del nostre entorn és multisensorial, és a dir, involucra el processament de senyals a través de diverses modalitats sensorials. Combinar aquesta informació en… (more)

▼ La percepció del nostre entorn és multisensorial, és a dir, involucra el processament de senyals a través de diverses modalitats sensorials. Combinar aquesta informació en el cervell per tal de formar una percepció coherent i integrada és un procés complex, degut a la diferent naturalesa de les senyals. A més, això farà que el cervell hagi de resoldre diferències temporals durant el processament de la informació. En els últims anys, ha sorgit un profund interès per entendre com el sistema perceptiu genera la impressió de sincronia d’estímuls provinents de diferents modalitats sensorials. La major part dels estudis han examinat propietats de la percepció de sincronia relacionades directament amb els estímuls físics, en contexts molt simplificats. En aquesta tesi investigo la influència de factors cognitius i de l’estat intern de l’individu (com per exemple l’atenció, demandes en tasques motores, i els ritmes interns cerebrals) en la percepció de sincronia entre estímuls audiovisuals. En els primers dos estudis de la tesi, hem examinat la funció de l’atenció i les accions durant la recalibració temporal d’estímuls audiovisuals. Els resultats dels estudis mostren com la sincronia subjectiva pot ser fortament modulada en funció d’on es dirigeixi el focus atencional del participant, en condicions on l’estimulació física és idèntica. En el tercer estudi, hem enregistrat l’activitat electroencefalogràfica dels participants, mentres realitzaven una tasca de simultaneïtat. Durant aquesta tasca presentàvem diferentes asincronies entre estímuls audiovisuals per tal d’estudiar la percepció de sincronia (vs. asincronia). Els resultats indiquen que la fase de les oscil·lacions neuronals, que reflecteixen estats cerebrals abans de la presència d’un estímul audiovisual, poden predir la resposta en quan a percepció de sincronia. En resum, els nostres resultats aporten coneixement sobre com alguns factors cognitius poden modular la percepció multisensorial. Advisors/Committee Members: [email protected] (authoremail), true (authoremailshow), Burdío Pinilla, Fernando (director), true (authorsendemail).

Subjects/Keywords: Irreversible electroporation; Liver tumors; Metastases; Electroporación irreversible; Tumores hepáticos; Metástasis; 616

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Sánchez Velázquez, Patricia, 1. (2017). Effect of the irreversible electroporation in a murine model of colorectal liver metastases. (Thesis). Universitat Pompeu Fabra. Retrieved from http://hdl.handle.net/10803/565772

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Sánchez Velázquez, Patricia, 1985-. “Effect of the irreversible electroporation in a murine model of colorectal liver metastases.” 2017. Thesis, Universitat Pompeu Fabra. Accessed January 15, 2021. http://hdl.handle.net/10803/565772.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Sánchez Velázquez, Patricia, 1985-. “Effect of the irreversible electroporation in a murine model of colorectal liver metastases.” 2017. Web. 15 Jan 2021.

Vancouver:

Sánchez Velázquez, Patricia 1. Effect of the irreversible electroporation in a murine model of colorectal liver metastases. [Internet] [Thesis]. Universitat Pompeu Fabra; 2017. [cited 2021 Jan 15]. Available from: http://hdl.handle.net/10803/565772.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Sánchez Velázquez, Patricia 1. Effect of the irreversible electroporation in a murine model of colorectal liver metastases. [Thesis]. Universitat Pompeu Fabra; 2017. Available from: http://hdl.handle.net/10803/565772

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Universitat Pompeu Fabra

5. Mercadal Cavaller, Borja. Electroporation and peripheral nerve stimulation.

Degree: Departament de Tecnologies de la Informació i les Comunicacions, 2019, Universitat Pompeu Fabra

URL: http://hdl.handle.net/10803/667854

► Aquesta tesi té com a objectiu resoldre qüestions en els camps de l’electroporació i l’estimulació dels nervis perifèrics, i sobretot, aquelles que es deriven de… (more)

Subjects/Keywords: Electroporation; Electrical stimulation; Neuromuscular; Irreversible electroporation; Bipolar pulses; High-Frequency irreversible electroporation; Pulsed radiofrequency; Intramuscular stimulation; 62

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APA (6^th Edition):

Mercadal Cavaller, B. (2019). Electroporation and peripheral nerve stimulation. (Thesis). Universitat Pompeu Fabra. Retrieved from http://hdl.handle.net/10803/667854

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Mercadal Cavaller, Borja. “Electroporation and peripheral nerve stimulation.” 2019. Thesis, Universitat Pompeu Fabra. Accessed January 15, 2021. http://hdl.handle.net/10803/667854.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Mercadal Cavaller, Borja. “Electroporation and peripheral nerve stimulation.” 2019. Web. 15 Jan 2021.

Vancouver:

Mercadal Cavaller B. Electroporation and peripheral nerve stimulation. [Internet] [Thesis]. Universitat Pompeu Fabra; 2019. [cited 2021 Jan 15]. Available from: http://hdl.handle.net/10803/667854.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Mercadal Cavaller B. Electroporation and peripheral nerve stimulation. [Thesis]. Universitat Pompeu Fabra; 2019. Available from: http://hdl.handle.net/10803/667854

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Virginia Tech

6. Colacino, Katelyn. The Potential of Cellulose Nanocrystals in the Detection and Treatment of Cancer.

Degree: PhD, Biomedical Engineering, 2013, Virginia Tech

URL: http://hdl.handle.net/10919/51212

► Conventional methods of cancer therapy have been severely limited by inefficient delivery of therapeutic doses without incidence of harsh and toxic side effects in normal… (more)

▼ Conventional methods of cancer therapy have been severely limited by inefficient delivery of therapeutic doses without incidence of harsh and toxic side effects in normal tissues. Consequently, countless new methods for early detection and drug delivery have been investigated in the area of nanoparticles and hydrogels. Although many of these methods are promising, the complex nature of cancer increases the difficultly for the development of the perfect system. Cellulose nanocrystals (CNCs) have been studied widely for a variety of applications. Despite their advantages, investigations of their abilities in the biomedical field have not been explored. The goal of this project is to delve into the potential uses of CNCs in detection, targeted drug delivery, and potentiation of irreversible electroporation (IRE)-induced cell death in folate receptor (FR)-positive cancers. To accomplish this task we have prepared stable and reproducible CNCs from wood pulp via sulfuric acid hydrolysis. Furthermore, we have functionalized the surface of these nanoparticles and conjugated them with the targeting ligand folic acid (FA) and the fluorescent imaging agent fluorescein-5\'-isothiocyanate (FITC) to create FITC-CNC-FA; CNCs have also been conjugated with doxorubicin (DOX), a potent chemotherapeutic (DOX-ALAL-CNC-FA). We have determined FITC-CNC-FA\'s and DOX-ALAL-CNC-FA\'s ability to specifically target FR-positive cancer cells in vitro; meanwhile non-targeted CNCs (FITC-CNC) were shown unable to bind to these cell types. In addition, we have investigated FITC-CNC-FA\'s pharmacokinetic activity in vivo. To properly model the CNC conjugate\'s activity in vivo, a physiologically based pharmacokinetic (PBPK) model has been constructed. We have also examined CNCsábility to potentiate a new technique for tumor ablation, IRE. Pre-incubation with FA-conjugated CNCs (CNC-FA) have shown an increase in cytotoxicity in FR-positive cancer cells induced by IRE. In addition, CNC-FA did not potentiate IRE-induced cytotoxicity in a FR-negative cancer cell type. For a more comprehensive understanding of CNC-FA\'s ability to potentiate IRE induced cytotoxicity, we optimized a 3D in vitro hydrogel system. Preliminary data suggest this method of experimentation will be more realistic to in vivo studies to be completed in the future. Together, these studies showcase CNCs as efficient and effective nano-carriers in tumor detection and treatment. Advisors/Committee Members: Lee, Yong Woo (committeechair), Achenie, Luke E. K. (committee member), Roman, Maren (committee member), Rylander, M Nichole (committee member), Robertson, John L. (committee member).

Subjects/Keywords: Folate Receptor; Early Cancer Detection; Targeted Drug Delivery; Irreversible Electroporation

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Colacino, K. (2013). The Potential of Cellulose Nanocrystals in the Detection and Treatment of Cancer. (Doctoral Dissertation). Virginia Tech. Retrieved from http://hdl.handle.net/10919/51212

Chicago Manual of Style (16^th Edition):

Colacino, Katelyn. “The Potential of Cellulose Nanocrystals in the Detection and Treatment of Cancer.” 2013. Doctoral Dissertation, Virginia Tech. Accessed January 15, 2021. http://hdl.handle.net/10919/51212.

MLA Handbook (7^th Edition):

Colacino, Katelyn. “The Potential of Cellulose Nanocrystals in the Detection and Treatment of Cancer.” 2013. Web. 15 Jan 2021.

Vancouver:

Colacino K. The Potential of Cellulose Nanocrystals in the Detection and Treatment of Cancer. [Internet] [Doctoral dissertation]. Virginia Tech; 2013. [cited 2021 Jan 15]. Available from: http://hdl.handle.net/10919/51212.

Council of Science Editors:

Colacino K. The Potential of Cellulose Nanocrystals in the Detection and Treatment of Cancer. [Doctoral Dissertation]. Virginia Tech; 2013. Available from: http://hdl.handle.net/10919/51212

University of Minnesota

7. Pelaez, Francisco. Biomaterial Scaffolds as a Platform for Focal Therapy Against Disseminated Cancer Cells.

Degree: PhD, Chemical Engineering, 2018, University of Minnesota

URL: http://hdl.handle.net/11299/209126

► The primary cause of death for cancer patients stems not from the primary tumor, but from the spread of the disease to distal sites. Once… (more)

▼ The primary cause of death for cancer patients stems not from the primary tumor, but from the spread of the disease to distal sites. Once the cancer has become detectable past the point of origin, tumor burden in vital organs is typically too high, limiting the efficacy of current treatment options. Development of novel therapies to target disseminated cancer cells prior to the onset of extensive metastatic burden could improve patient outcomes. Focal therapy, or energy-based ablation, has been successfully used to treat solid tumors, but has yet to be effectively applied toward disseminated cells. Microporous polymer scaffolds have previously been developed to capture metastatic breast cancer cells in vivo, and have been shown to reduce tumor burden in vital organs and improve survival. The purpose of this dissertation is to further improve the therapeutic potential of these biomaterials by destroying recruited cancer cells. Chapter 1 discusses in further detail how these scaffolds recruit metastatic cancer cells and how different types of focal therapy have been used to treat solid tumors in the clinic. To demonstrate that focal therapy could be used to kill cells within the scaffold, metal disks were integrated into polymer scaffolds to serve as a heat source through induction heating for focal hyperthermia. It was shown in Chapter 2 that these modified scaffolds could be applied successfully to kill infiltrating cells in vivo. The release of cancer-specific antigens following ablation of metastatic cells could be utilized to induce an anti-cancer systemic immune response. Utilizing melanocytes because of their well-defined tumorigenic antigens, collaborative work demonstrated that lysates generated from ablation via irreversible electroporation (IRE) resulted in the greatest amount of T cell activation in vitro in comparison to focal hyperthermia or cryogenic ablation. Thus, for potential future combinatorial applications with immunotherapy, Chapter 3 investigated methods to introduce IRE to polymer scaffolds to treat disseminated cells. To accomplish this, metal mesh electrodes were integrated on either side of polymer scaffolds to create a near-uniform electric field distribution. These modified scaffolds were shown to be capable of complete ablation of infiltrating cells upon IRE in vivo in some mice, although further refinement is required to eliminate the variability in the response. The amount of antigen release from the treatment of recruited cells may be insufficient to induce a robust immune response following IRE treatment, and the local delivery of adjuvants may be required. Chapter 4 explored how polymer scaffolds could be coupled with inducible release of small molecules after electroporation. Scaffolds were modified to facilitate conjugation of inducible drug carriers and properties affecting IRE-mediated release were investigated to identify optimal conditions. In Chapter 5, polymer scaffolds were evaluated for recruitment of melanoma cells in both experimental and spontaneous metastasis models…

Subjects/Keywords: Cancer therapy; Composite scaffold; Induction heating; Irreversible electroporation; Metastasis

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Pelaez, F. (2018). Biomaterial Scaffolds as a Platform for Focal Therapy Against Disseminated Cancer Cells. (Doctoral Dissertation). University of Minnesota. Retrieved from http://hdl.handle.net/11299/209126

Chicago Manual of Style (16^th Edition):

Pelaez, Francisco. “Biomaterial Scaffolds as a Platform for Focal Therapy Against Disseminated Cancer Cells.” 2018. Doctoral Dissertation, University of Minnesota. Accessed January 15, 2021. http://hdl.handle.net/11299/209126.

MLA Handbook (7^th Edition):

Pelaez, Francisco. “Biomaterial Scaffolds as a Platform for Focal Therapy Against Disseminated Cancer Cells.” 2018. Web. 15 Jan 2021.

Vancouver:

Pelaez F. Biomaterial Scaffolds as a Platform for Focal Therapy Against Disseminated Cancer Cells. [Internet] [Doctoral dissertation]. University of Minnesota; 2018. [cited 2021 Jan 15]. Available from: http://hdl.handle.net/11299/209126.

Council of Science Editors:

Pelaez F. Biomaterial Scaffolds as a Platform for Focal Therapy Against Disseminated Cancer Cells. [Doctoral Dissertation]. University of Minnesota; 2018. Available from: http://hdl.handle.net/11299/209126

University of Minnesota

8. Jiang, Chunlan. Membrane-Targeting Approaches for Enhanced Cell Destruction with Irreversible Electroporation.

Degree: PhD, Mechanical Engineering, 2014, University of Minnesota

URL: http://hdl.handle.net/11299/173934

► Irreversible Electroporation (IRE) has gained increasing popularity in the cancer treatment field during the past decade due to many advantages over other focal therapies. Despite… (more)

▼ Irreversible Electroporation (IRE) has gained increasing popularity in the cancer treatment field during the past decade due to many advantages over other focal therapies. Despite early success in pre-clinical and clinical IRE trials, in vivo studies have shown that IRE suffers from an inability to destroy large volumes of cancer tissue without repeating treatment and/or increasing the applied electrical dose to dangerous levels. There are approaches to expand the treatment volume by IRE with the addition of chemotherapeutic or cytotoxic agents. While these studies demonstrated improved cell killing, the focus was on enhancing the ability of chemotherapeutic drugs or cytotoxic agents to enter and kill the cancer cells rather than enhancing the efficacy of IRE itself. Therefore, the aim of this work is to investigate the ability to increase the destructive capability of IRE without relying on cytotoxic drugs. Specifically, mechanisms that directly modify membrane properties should reduce the voltage threshold for lethal permeabilization and therefore increase the efficacy of cell killing and therefore the volume treated after a given IRE level. Two methods to achieve these changes are proposed in this study: 1) addition of surfactant (e.g. Dimethyl sulfoxide, or DMSO) to directly interact with membrane lipids thereby changing membrane line tension and surface tension, and 2) use of pulse timing (i.e. introduction and persistence of defects in the membrane between pulses). Here then we began by Investigation of IRE enhancement in vitro to understand the impacts of our proposed mechanisms and their ideal working parameters. We found that the best enhancement effect was achieved with addition of 5% v/v DMSO, which resulted in a significant increase of 75% more cell destruction compared to baseline IRE. Similarly with pulse timing, when dividing the pulses into three trains with 30s delays in between, an enhancement of 67% more cell destruction was achieved compared to baseline IRE. Next we tested our IRE enhancement approaches in an in vivo dorsal skin fold chamber (DSFC) model of prostate cancer with optimal parameters selected from our in vitro experiments. The results reproducibly showed that more than 120% and 101% enhancement in the treatment volume were achieved by the addition of DMSO and pulse timing, respectively, with two independent injury assessment methods (histological and perfusion defect). Finally, we translated one of the enhancement approaches (pulse timing) to an in vivo hind limb model of prostate cancer and demonstrated that more than 33% additional tumor destruction and 2 weeks longer tumor growth delay could be achieved compared to baseline IRE treatment without relying on any cytotoxic drugs or agents. Because DMSO is commercially available and regularly used at low concentrations (<10% v/v) in clinic, this approach could easily be integrated into current IRE procedures to increase the treatment efficacy. In addition, introducing pulse timing delays in IRE also increases the destructive potential…

Subjects/Keywords: Adjuvant; DMSO; Enhancement; Irreversible Electroporation; Membrane; Pulse Timing

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APA (6^th Edition):

Jiang, C. (2014). Membrane-Targeting Approaches for Enhanced Cell Destruction with Irreversible Electroporation. (Doctoral Dissertation). University of Minnesota. Retrieved from http://hdl.handle.net/11299/173934

Chicago Manual of Style (16^th Edition):

Jiang, Chunlan. “Membrane-Targeting Approaches for Enhanced Cell Destruction with Irreversible Electroporation.” 2014. Doctoral Dissertation, University of Minnesota. Accessed January 15, 2021. http://hdl.handle.net/11299/173934.

MLA Handbook (7^th Edition):

Jiang, Chunlan. “Membrane-Targeting Approaches for Enhanced Cell Destruction with Irreversible Electroporation.” 2014. Web. 15 Jan 2021.

Vancouver:

Jiang C. Membrane-Targeting Approaches for Enhanced Cell Destruction with Irreversible Electroporation. [Internet] [Doctoral dissertation]. University of Minnesota; 2014. [cited 2021 Jan 15]. Available from: http://hdl.handle.net/11299/173934.

Council of Science Editors:

Jiang C. Membrane-Targeting Approaches for Enhanced Cell Destruction with Irreversible Electroporation. [Doctoral Dissertation]. University of Minnesota; 2014. Available from: http://hdl.handle.net/11299/173934

Virginia Tech

9. Reese, Laura Michelle. Optimizing Emerging Healthcare Innovations in 3D Printing, Nanomedicine, and Imageable Biomaterials.

Degree: MS, Biomedical Engineering, 2015, Virginia Tech

URL: http://hdl.handle.net/10919/51539

► Emerging technologies in the healthcare industry encompass revolutionary devices or drugs that have the potential to change how healthcare will be practiced in the future.… (more)

▼ Emerging technologies in the healthcare industry encompass revolutionary devices or drugs that have the potential to change how healthcare will be practiced in the future. While there are several emerging healthcare technologies in the pipeline, a few key innovations are slated to be implemented clinically sooner based on their mass appeal and potential for healthcare breakthroughs. This thesis will focus on specific topics in the emerging technological fields of nanotechnology for photothermal cancer therapy, 3D printing for irreversible electroporation applications, and imageable biomaterials. While these general areas are receiving significant attention, we highlight the potential opportunities and limitations presented by our select efforts in these fields. First, in the realm of nanomedicine, we discuss the optimization and characterization of sodium thiosulfate facilitated gold nanoparticle synthesis. While many nanoparticles have been examined as agents for photothermal cancer therapy, we closely examine the structure and composition of these specific nanomaterials and discuss key findings that not only impact their future clinical use, but elucidate the importance of characterization prior to preclinical testing. Next, we examine the potential use of 3D printing to generate unprecedented multimodal medical devices for local pancreatic cancer therapy. This additive manufacturing technique offers exquisite design detail control, facilitating tools that would otherwise be difficult to fabricate by any other means. Lastly, in the field of imageable biomaterials, we demonstrate the development of composite catheters that can be visualized with near infrared imaging. This new biomaterial allows visualization with near infrared imaging, offering potentially new medical device opportunities that alleviate the use of ionizing radiation. This collective work emphasizes the need to thoroughly optimize and characterize emerging technologies prior to preclinical testing in order to facilitate rapid translation. Advisors/Committee Members: Bickford, Lissett R. (committeechair), Lee, Yong Woo (committee member), Whittington, Abby R. (committee member).

Subjects/Keywords: Irreversible Electroporation; Additive Manufacturing; Near Infrared Imaging; Gold Nanoparticles

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APA (6^th Edition):

Reese, L. M. (2015). Optimizing Emerging Healthcare Innovations in 3D Printing, Nanomedicine, and Imageable Biomaterials. (Masters Thesis). Virginia Tech. Retrieved from http://hdl.handle.net/10919/51539

Chicago Manual of Style (16^th Edition):

Reese, Laura Michelle. “Optimizing Emerging Healthcare Innovations in 3D Printing, Nanomedicine, and Imageable Biomaterials.” 2015. Masters Thesis, Virginia Tech. Accessed January 15, 2021. http://hdl.handle.net/10919/51539.

MLA Handbook (7^th Edition):

Reese, Laura Michelle. “Optimizing Emerging Healthcare Innovations in 3D Printing, Nanomedicine, and Imageable Biomaterials.” 2015. Web. 15 Jan 2021.

Vancouver:

Reese LM. Optimizing Emerging Healthcare Innovations in 3D Printing, Nanomedicine, and Imageable Biomaterials. [Internet] [Masters thesis]. Virginia Tech; 2015. [cited 2021 Jan 15]. Available from: http://hdl.handle.net/10919/51539.

Council of Science Editors:

Reese LM. Optimizing Emerging Healthcare Innovations in 3D Printing, Nanomedicine, and Imageable Biomaterials. [Masters Thesis]. Virginia Tech; 2015. Available from: http://hdl.handle.net/10919/51539

10. Oshin, Edwin Ayobami. Impedance Analysis of Tissues in nsPEF Treatment for Cancer Therapy.

Degree: MS, 2020, Old Dominion University

URL: 9798641036434 ; https://digitalcommons.odu.edu/biomedengineering_etds/11

► Nanosecond pulsed electric field (nsPEF) for cancer therapy is characterized by applications of high voltage pulses with low pulsed energy to induce non-thermal effects… (more)

Subjects/Keywords: Ablation; Bioimpedance; Electroporation; Impedance sensor; Irreversible electroporation; Tumor therapy; Bioinformatics; Biomedical Engineering and Bioengineering; Biophysics; Oncology

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APA (6^th Edition):

Oshin, E. A. (2020). Impedance Analysis of Tissues in nsPEF Treatment for Cancer Therapy. (Thesis). Old Dominion University. Retrieved from 9798641036434 ; https://digitalcommons.odu.edu/biomedengineering_etds/11

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Oshin, Edwin Ayobami. “Impedance Analysis of Tissues in nsPEF Treatment for Cancer Therapy.” 2020. Thesis, Old Dominion University. Accessed January 15, 2021. 9798641036434 ; https://digitalcommons.odu.edu/biomedengineering_etds/11.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Oshin, Edwin Ayobami. “Impedance Analysis of Tissues in nsPEF Treatment for Cancer Therapy.” 2020. Web. 15 Jan 2021.

Vancouver:

Oshin EA. Impedance Analysis of Tissues in nsPEF Treatment for Cancer Therapy. [Internet] [Thesis]. Old Dominion University; 2020. [cited 2021 Jan 15]. Available from: 9798641036434 ; https://digitalcommons.odu.edu/biomedengineering_etds/11.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Oshin EA. Impedance Analysis of Tissues in nsPEF Treatment for Cancer Therapy. [Thesis]. Old Dominion University; 2020. Available from: 9798641036434 ; https://digitalcommons.odu.edu/biomedengineering_etds/11

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

University of Minnesota

11. Elahi-Gedwillo, Kianna. Stroma-targeting therapies and local intervention in pancreatic ductal adenocarcinoma.

Degree: PhD, Biomedical Engineering, 2018, University of Minnesota

URL: http://hdl.handle.net/11299/200197

► Pancreatic ductal adenocarcinoma (PDA) remains one of the deadliest forms of cancer, in part, because it is largely refractory to current therapies. The failure of… (more)

▼ Pancreatic ductal adenocarcinoma (PDA) remains one of the deadliest forms of cancer, in part, because it is largely refractory to current therapies. The failure of most standard therapies, as well as promising immune therapies, may be largely ascribed to a highly unique and protective stromal microenvironment that presents significant biophysical barriers to effective drug delivery, as well as distribution and function of anti-tumor immune cells. This work supports a growing body of evidence implicating the critical nature of the stroma in tumor progression and resistance to therapy, and demonstrates how disruption of the stroma through focused stromal re-engineering approaches likely represents a key component of viable strategies to treat PDA with molecular and immune therapies. In addition to systemic therapies to combat PDA, local therapies are emerging as promising alternatives. Here, we investigate irreversible electroporation (IRE), a nonthermal focal therapy technique that induces widespread necrosis while sparing local blood vessels. These key characteristics make IRE an ideal treatment modality in the physiologically sensitive context of the pancreas, and may represent an unexplored approach to be used in concert with systemic therapies, such as stroma-targeting therapy (STT). In Chapter 1, we lay the groundwork to understand the unique pathology of PDA and the relatively recent—and at times, controversial—history of STT. We review the antifibrotic agent, halofuginone (HF), which is utilized in the majority of the preclinical intervention studies presented in this dissertation. Complementary to systemic STT approaches explored here, we describe electroporation, a nonthermal focal therapy technique which is widely used in the clinic in combination with chemotherapy, but has been proposed as a direct and monotherapeutic approach to ablate tissue in cancer within the last decade or so. Finally, we review models historically used to study novel therapeutics in PDA, and the predictive utility of each. In Chapter 2, the experimental methods used to conduct these studies are described in detail, including the genetically-engineered KPC mouse model our lab employs and the associated intervention studies that these mice were enrolled in. In Chapter 3, we present the results of our effort to disrupt the severe biophysical barriers characteristic of PDA through stromal re-engineering. This was largely done using the potent antifibrotic agent, HF. In an autochthonous, genetically engineered mouse model of PDA, we show that HF disrupts barriers to effective drug distribution and also results in increased number and distribution of cytotoxic T cells. Furthermore, we show that HF treatment directly targets carcinoma cells and leads to widespread intratumoral necrosis and reduced tumor volume. These data point to the multifunctional and critical role of the stroma in tumor protection and survival, and demonstrate how compromising tumor integrity through STT will likely be an instrumental component in…

Subjects/Keywords: drug transport and resistance; irreversible electroporation; pancreatic cancer; stroma targeting therapy; tumor and immune microenvironment

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APA (6^th Edition):

Elahi-Gedwillo, K. (2018). Stroma-targeting therapies and local intervention in pancreatic ductal adenocarcinoma. (Doctoral Dissertation). University of Minnesota. Retrieved from http://hdl.handle.net/11299/200197

Chicago Manual of Style (16^th Edition):

Elahi-Gedwillo, Kianna. “Stroma-targeting therapies and local intervention in pancreatic ductal adenocarcinoma.” 2018. Doctoral Dissertation, University of Minnesota. Accessed January 15, 2021. http://hdl.handle.net/11299/200197.

MLA Handbook (7^th Edition):

Elahi-Gedwillo, Kianna. “Stroma-targeting therapies and local intervention in pancreatic ductal adenocarcinoma.” 2018. Web. 15 Jan 2021.

Vancouver:

Elahi-Gedwillo K. Stroma-targeting therapies and local intervention in pancreatic ductal adenocarcinoma. [Internet] [Doctoral dissertation]. University of Minnesota; 2018. [cited 2021 Jan 15]. Available from: http://hdl.handle.net/11299/200197.

Council of Science Editors:

Elahi-Gedwillo K. Stroma-targeting therapies and local intervention in pancreatic ductal adenocarcinoma. [Doctoral Dissertation]. University of Minnesota; 2018. Available from: http://hdl.handle.net/11299/200197

Universitat Pompeu Fabra

12. José Segarra-Martínez, Anabel. Novel approaches against pancreatic cancer based on adenoviral targeting and tumor ablation preclinical evaluation of antitumor efficacy.

Degree: Departament de Ciències Experimentals i de la Salut, 2011, Universitat Pompeu Fabra

URL: http://hdl.handle.net/10803/83345

► Novel therapies are needed to overcome the limited efficacy of current treatments in pancreatic cancer. Adenoviral gene therapy against pancreatic tumors is challenged by the… (more)

Subjects/Keywords: càncer de pàncrees; teràpia gènica; adenovirus; electroporació irreversible; injecció intraductal; redireccionament transduccional; gemcitabina; Pancreatic cancer; gene therapy; adenovirus; irreversible electroporation; intraductal injection; transductional targeting; gemcitabine; 616

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APA (6^th Edition):

José Segarra-Martínez, A. (2011). Novel approaches against pancreatic cancer based on adenoviral targeting and tumor ablation preclinical evaluation of antitumor efficacy. (Thesis). Universitat Pompeu Fabra. Retrieved from http://hdl.handle.net/10803/83345

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

José Segarra-Martínez, Anabel. “Novel approaches against pancreatic cancer based on adenoviral targeting and tumor ablation preclinical evaluation of antitumor efficacy.” 2011. Thesis, Universitat Pompeu Fabra. Accessed January 15, 2021. http://hdl.handle.net/10803/83345.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

José Segarra-Martínez, Anabel. “Novel approaches against pancreatic cancer based on adenoviral targeting and tumor ablation preclinical evaluation of antitumor efficacy.” 2011. Web. 15 Jan 2021.

Vancouver:

José Segarra-Martínez A. Novel approaches against pancreatic cancer based on adenoviral targeting and tumor ablation preclinical evaluation of antitumor efficacy. [Internet] [Thesis]. Universitat Pompeu Fabra; 2011. [cited 2021 Jan 15]. Available from: http://hdl.handle.net/10803/83345.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

José Segarra-Martínez A. Novel approaches against pancreatic cancer based on adenoviral targeting and tumor ablation preclinical evaluation of antitumor efficacy. [Thesis]. Universitat Pompeu Fabra; 2011. Available from: http://hdl.handle.net/10803/83345

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Universitat Pompeu Fabra

13. Pañella Vilamú, Clara, 1987-. Focused transhepatic electroporation mediated by hypersaline infusion through the portal vein in murine model : Preliminary results on differential conductivity and safety.

Degree: Departament de Ciències Experimentals i de la Salut, 2019, Universitat Pompeu Fabra

URL: http://hdl.handle.net/10803/668272

► INTRODUCTION: This study evaluates the selective increase in liver conductivity by hypersaline infusion through the portomesenteric system in order to treat all the hepatic tumors… (more)

Subjects/Keywords: Electroporación irreversible; Tumor hepático; Conductividad eléctrica; Infusión hipersalina y metástasis hepáticas; Irreversible electroporation; Liver tumor; Electric conductivity; Hipersaline infusion and liver metastases; 616.3

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APA (6^th Edition):

Pañella Vilamú, Clara, 1. (2019). Focused transhepatic electroporation mediated by hypersaline infusion through the portal vein in murine model : Preliminary results on differential conductivity and safety. (Thesis). Universitat Pompeu Fabra. Retrieved from http://hdl.handle.net/10803/668272

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Pañella Vilamú, Clara, 1987-. “Focused transhepatic electroporation mediated by hypersaline infusion through the portal vein in murine model : Preliminary results on differential conductivity and safety.” 2019. Thesis, Universitat Pompeu Fabra. Accessed January 15, 2021. http://hdl.handle.net/10803/668272.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Pañella Vilamú, Clara, 1987-. “Focused transhepatic electroporation mediated by hypersaline infusion through the portal vein in murine model : Preliminary results on differential conductivity and safety.” 2019. Web. 15 Jan 2021.

Vancouver:

Pañella Vilamú, Clara 1. Focused transhepatic electroporation mediated by hypersaline infusion through the portal vein in murine model : Preliminary results on differential conductivity and safety. [Internet] [Thesis]. Universitat Pompeu Fabra; 2019. [cited 2021 Jan 15]. Available from: http://hdl.handle.net/10803/668272.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Pañella Vilamú, Clara 1. Focused transhepatic electroporation mediated by hypersaline infusion through the portal vein in murine model : Preliminary results on differential conductivity and safety. [Thesis]. Universitat Pompeu Fabra; 2019. Available from: http://hdl.handle.net/10803/668272

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Brno University of Technology

14. Nohel, Michal. Synchronizace elektroporačních pulzů se srdečním rytmem: EKG synchonization of electroporation pulses.

Degree: 2019, Brno University of Technology

URL: http://hdl.handle.net/11012/173637

► Bachelor thesis is focused on cells electroporation principle and its types. It further describes thermal and nonthermal ablation techniques with an emphasis on irreversible electroporation.… (more)

Subjects/Keywords: elektroporace; ireverzibilní elektroporace; synchronizace; ablace; kardiologie; EKG; real-time detekce QRS; electroporation; irreversible electroporation; synchronization; ablation; cardiology; ECG; real-time detection of QRS

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APA (6^th Edition):

Nohel, M. (2019). Synchronizace elektroporačních pulzů se srdečním rytmem: EKG synchonization of electroporation pulses. (Thesis). Brno University of Technology. Retrieved from http://hdl.handle.net/11012/173637

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Nohel, Michal. “Synchronizace elektroporačních pulzů se srdečním rytmem: EKG synchonization of electroporation pulses.” 2019. Thesis, Brno University of Technology. Accessed January 15, 2021. http://hdl.handle.net/11012/173637.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Nohel, Michal. “Synchronizace elektroporačních pulzů se srdečním rytmem: EKG synchonization of electroporation pulses.” 2019. Web. 15 Jan 2021.

Vancouver:

Nohel M. Synchronizace elektroporačních pulzů se srdečním rytmem: EKG synchonization of electroporation pulses. [Internet] [Thesis]. Brno University of Technology; 2019. [cited 2021 Jan 15]. Available from: http://hdl.handle.net/11012/173637.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Nohel M. Synchronizace elektroporačních pulzů se srdečním rytmem: EKG synchonization of electroporation pulses. [Thesis]. Brno University of Technology; 2019. Available from: http://hdl.handle.net/11012/173637

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Virginia Tech

15. Thomas, Sean Casey. A Developed and Characterized Orthotopic Rat Glioblastoma Multiforme Model.

Degree: MS, Biomedical Engineering, 2020, Virginia Tech

URL: http://hdl.handle.net/10919/100772

► Treating glioblastoma multiforme (GBM), a form of cancer found in the brain, has not been very successful; patients rarely live two years following diagnosis, and… (more)

Subjects/Keywords: high-frequency irreversible electroporation; blood-brain barrier disruption; molecular targeted therapy; bioluminescent imaging; glioblastoma multiforme; tumor ablation; cancer therapy; electroporation; drug delivery; ablation; rat model

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APA (6^th Edition):

Thomas, S. C. (2020). A Developed and Characterized Orthotopic Rat Glioblastoma Multiforme Model. (Masters Thesis). Virginia Tech. Retrieved from http://hdl.handle.net/10919/100772

Chicago Manual of Style (16^th Edition):

Thomas, Sean Casey. “A Developed and Characterized Orthotopic Rat Glioblastoma Multiforme Model.” 2020. Masters Thesis, Virginia Tech. Accessed January 15, 2021. http://hdl.handle.net/10919/100772.

MLA Handbook (7^th Edition):

Thomas, Sean Casey. “A Developed and Characterized Orthotopic Rat Glioblastoma Multiforme Model.” 2020. Web. 15 Jan 2021.

Vancouver:

Thomas SC. A Developed and Characterized Orthotopic Rat Glioblastoma Multiforme Model. [Internet] [Masters thesis]. Virginia Tech; 2020. [cited 2021 Jan 15]. Available from: http://hdl.handle.net/10919/100772.

Council of Science Editors:

Thomas SC. A Developed and Characterized Orthotopic Rat Glioblastoma Multiforme Model. [Masters Thesis]. Virginia Tech; 2020. Available from: http://hdl.handle.net/10919/100772

Brno University of Technology

16. Novotná, Veronika. Analýza elektrických a tepelných jevů při elektroporaci: Analysis of electrical and thermal effects during electroporation.

Degree: 2020, Brno University of Technology

URL: http://hdl.handle.net/11012/184112

► This dissertation thesis describes a phenomenon called electroporation. It is about its theoretical aspects as well as about modeling of processes in the tissue during… (more)

Subjects/Keywords: Ablační techniky; ireverzibilní elektroporace; modelování; Pennesova rovnice; reverzibilní elektroporace; vysokonapěťový zdroj; Ablation techniques; irreversible electroporation; modeling; Pennes bioheat equation; reversible electroporation; high-voltage generator

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APA (6^th Edition):

Novotná, V. (2020). Analýza elektrických a tepelných jevů při elektroporaci: Analysis of electrical and thermal effects during electroporation. (Thesis). Brno University of Technology. Retrieved from http://hdl.handle.net/11012/184112

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Novotná, Veronika. “Analýza elektrických a tepelných jevů při elektroporaci: Analysis of electrical and thermal effects during electroporation.” 2020. Thesis, Brno University of Technology. Accessed January 15, 2021. http://hdl.handle.net/11012/184112.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Novotná, Veronika. “Analýza elektrických a tepelných jevů při elektroporaci: Analysis of electrical and thermal effects during electroporation.” 2020. Web. 15 Jan 2021.

Vancouver:

Novotná V. Analýza elektrických a tepelných jevů při elektroporaci: Analysis of electrical and thermal effects during electroporation. [Internet] [Thesis]. Brno University of Technology; 2020. [cited 2021 Jan 15]. Available from: http://hdl.handle.net/11012/184112.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Novotná V. Analýza elektrických a tepelných jevů při elektroporaci: Analysis of electrical and thermal effects during electroporation. [Thesis]. Brno University of Technology; 2020. Available from: http://hdl.handle.net/11012/184112

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

17. Zhang, Yue. Magnetic Resonance Imaging for Monitoring Irreversible Electroporation of Hepatocellular Carcinoma.

Degree: 2013, University of Illinois – Chicago

URL: http://hdl.handle.net/10027/10038

► Hepatocellular carcinoma (HCC) is the 6th most common neoplasm worldwidely and is 4th leading cause of cancer death in United States. It is estimated that… (more)

Subjects/Keywords: Magnetic Resonance Imaging; Irreversible Electroporation; Hepatocellular Carcinoma

…Gradient Recall Echo IRE Irreversible Electroporation HIFU High Intensity Focused-Ultrasound… …metastatic and original tumor. Irreversible electroporation (IRE) is a novel targeted… …2.2 IRREVERSIBLE ELECTROPORATION 2.2.1 Tumor Ablation Techniques The targeted tissue… …keep limiting overall efficacy. Irreversible electroporation (IRE) is a powerful… …ablation approaches. 7 2.2.2 Irreversible Electroporation Ablation 2.2.2.1…

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APA (6^th Edition):

Zhang, Y. (2013). Magnetic Resonance Imaging for Monitoring Irreversible Electroporation of Hepatocellular Carcinoma. (Thesis). University of Illinois – Chicago. Retrieved from http://hdl.handle.net/10027/10038

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Zhang, Yue. “Magnetic Resonance Imaging for Monitoring Irreversible Electroporation of Hepatocellular Carcinoma.” 2013. Thesis, University of Illinois – Chicago. Accessed January 15, 2021. http://hdl.handle.net/10027/10038.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Zhang, Yue. “Magnetic Resonance Imaging for Monitoring Irreversible Electroporation of Hepatocellular Carcinoma.” 2013. Web. 15 Jan 2021.

Vancouver:

Zhang Y. Magnetic Resonance Imaging for Monitoring Irreversible Electroporation of Hepatocellular Carcinoma. [Internet] [Thesis]. University of Illinois – Chicago; 2013. [cited 2021 Jan 15]. Available from: http://hdl.handle.net/10027/10038.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Zhang Y. Magnetic Resonance Imaging for Monitoring Irreversible Electroporation of Hepatocellular Carcinoma. [Thesis]. University of Illinois – Chicago; 2013. Available from: http://hdl.handle.net/10027/10038

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

18. Prokop, Katherine Jane. Cell Death Characterization In Tumor Constructs Using Irreversible Electroporation.

Degree: MS, Biomedical Engineering, 2013, Virginia Tech

URL: http://hdl.handle.net/10919/51655

► Pancreatic and prostate cancer are both prevalent cancers in the United States with pancreatic being one of the most aggressive of all cancers and prostate… (more)

Subjects/Keywords: irreversible electroporation; hydrogels; pancreatic cancer; prostate cancer

…pancreatic but for other cancer’s as well. Irreversible electroporation is a promising non-thermal… …permeabilization of the membrane becomes permanent in a process known as irreversible electroporation… …Irreversible electroporation (IRE) is a non-thermal ablation method to kill tumors… …that they cannot recover [5-7]. Irreversible electroporation involves the… …structurally changing the membrane leading to cell death. Irreversible electroporation offers…

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APA (6^th Edition):

Prokop, K. J. (2013). Cell Death Characterization In Tumor Constructs Using Irreversible Electroporation. (Masters Thesis). Virginia Tech. Retrieved from http://hdl.handle.net/10919/51655

Chicago Manual of Style (16^th Edition):

Prokop, Katherine Jane. “Cell Death Characterization In Tumor Constructs Using Irreversible Electroporation.” 2013. Masters Thesis, Virginia Tech. Accessed January 15, 2021. http://hdl.handle.net/10919/51655.

MLA Handbook (7^th Edition):

Prokop, Katherine Jane. “Cell Death Characterization In Tumor Constructs Using Irreversible Electroporation.” 2013. Web. 15 Jan 2021.

Vancouver:

Prokop KJ. Cell Death Characterization In Tumor Constructs Using Irreversible Electroporation. [Internet] [Masters thesis]. Virginia Tech; 2013. [cited 2021 Jan 15]. Available from: http://hdl.handle.net/10919/51655.

Council of Science Editors:

Prokop KJ. Cell Death Characterization In Tumor Constructs Using Irreversible Electroporation. [Masters Thesis]. Virginia Tech; 2013. Available from: http://hdl.handle.net/10919/51655

19. Phillips, Mary Alice. The Molecular Selectivity of Non-Thermal Irreversible Electroporation and Tissue Regeneration In Vivo.

Degree: Mechanical Engineering, 2012, University of California – Berkeley

URL: http://www.escholarship.org/uc/item/65z6h2hj

► Non-thermal irreversible electroporation (NTIRE) is new minimally-invasive surgical technique for tissue ablation that utilizes molecular selectivity to ablate tissue tumors. Short, microsecond electrical pulses are… (more)

▼ Non-thermal irreversible electroporation (NTIRE) is new minimally-invasive surgical technique for tissue ablation that utilizes molecular selectivity to ablate tissue tumors. Short, microsecond electrical pulses are applied to the tissue, selectively targeting the cell membrane, causing pores to form within the membrane and leading to cell death. This tissue ablation technique has potential for a variety of medical applications, and has shown great promise as a method for treating cancer tumors. NTIRE has many promising attributes as a treatment modality, such as the preservation of tissue scaffolding and the blood vessels. Very little work, however, has been done in examining how the molecular selectivity of NTIRE affects tissue regeneration. This work examines how tissues regenerate and recover after NTIRE, with a focus on those critical tissues that are particularly susceptible to collateral damage from treating an adjacent tumor. Two important tissues are examined: the artery and the small intestine. The artery may be embedded within a tumor. Although complete tumor ablation is desired, it is important that the artery can recover quickly in order to aid in overall tissue regeneration at the treated site. It is also important to understand how the molecular selectivity of NTIRE affects the regeneration of the small intestine, especially for the application of abdominal cancer treatment. Damage to the small intestine is often the limiting factor in other types of cancer treatments such as localized radiation therapy, causing pain and discomfort and even resulting in stopping the treatment early. Understanding how the small intestine recovers after NTIRE is essential in developing this technology for treating abdominal cancers such as pancreatic cancer. Finite element models were utilized to design electrical parameters for both the artery and the small intestine that would cause irreversible electroporation to occur within the tissue while avoiding thermal damage due to Joule heating effects. These electrical parameters were then applied in vivo. Electrical parameters chosen to apply to the artery were an electric field of 1750 V/cm, 90 pulses of a pulse length of 100 μs, and a frequency of either 1 or 4 Hz. The chosen small intestine electroporation protocol consisted of 2000 V/cm, 50 pulses of 70 μs each, and a frequency of 4 Hz. Additional finite element analysis was used to examine the effect of the heterogeneity of tissues such as the small intestine, indicating that changes in electrical conductivity from layer to layer is an important factor that should be accounted for in clinical treatment planning, and future work should include quantifying these electrical conductivity values.By applying NTIRE to the rat carotid artery, the recovery of the artery over the week following treatment was observed. It was demonstrated that the electroporation protocol preserved the native tissue extracellular matrix. Three days after NTIRE treatment, the ablated cells had been naturally removed from…

Subjects/Keywords: Mechanical engineering; Cancer ablation; Irreversible electroporation; Tissue engineering; Tissue regeneration

…electroporation on biological tissues with time. Currently irreversible electroporation is being… …developed as a method for ablating cancer tumors. Non-thermal irreversible electroporation (… …irreversible electroporation as it is further developed for other medical purposes. Here, the artery… …effect of irreversible electroporation on the artery is examined in the context of developing a… …technology, and a background of irreversible electroporation for cancer treatment as well as the…

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APA (6^th Edition):

Phillips, M. A. (2012). The Molecular Selectivity of Non-Thermal Irreversible Electroporation and Tissue Regeneration In Vivo. (Thesis). University of California – Berkeley. Retrieved from http://www.escholarship.org/uc/item/65z6h2hj

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Phillips, Mary Alice. “The Molecular Selectivity of Non-Thermal Irreversible Electroporation and Tissue Regeneration In Vivo.” 2012. Thesis, University of California – Berkeley. Accessed January 15, 2021. http://www.escholarship.org/uc/item/65z6h2hj.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Phillips, Mary Alice. “The Molecular Selectivity of Non-Thermal Irreversible Electroporation and Tissue Regeneration In Vivo.” 2012. Web. 15 Jan 2021.

Vancouver:

Phillips MA. The Molecular Selectivity of Non-Thermal Irreversible Electroporation and Tissue Regeneration In Vivo. [Internet] [Thesis]. University of California – Berkeley; 2012. [cited 2021 Jan 15]. Available from: http://www.escholarship.org/uc/item/65z6h2hj.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Phillips MA. The Molecular Selectivity of Non-Thermal Irreversible Electroporation and Tissue Regeneration In Vivo. [Thesis]. University of California – Berkeley; 2012. Available from: http://www.escholarship.org/uc/item/65z6h2hj

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Brno University of Technology

20. Kafka, Roman. Modelování tepelných účinků elektroporačního procesu na živou tkáň: Modeling of the thermal effects on living tissue during electroporation process.

Degree: 2020, Brno University of Technology

URL: http://hdl.handle.net/11012/190398

► The work deals with the current state of knowledge of heat transfer in living tissue. It is described here what the distribution of heat is… (more)

Subjects/Keywords: ireverzibilní elektroporace; teplo; přenos tepla; živá tkáň; tepelný model; Pennesova rovnice; irreversible electroporation; heat; heat transfer; living tissue; thermal model; Pennes equation

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Kafka, R. (2020). Modelování tepelných účinků elektroporačního procesu na živou tkáň: Modeling of the thermal effects on living tissue during electroporation process. (Thesis). Brno University of Technology. Retrieved from http://hdl.handle.net/11012/190398

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Kafka, Roman. “Modelování tepelných účinků elektroporačního procesu na živou tkáň: Modeling of the thermal effects on living tissue during electroporation process.” 2020. Thesis, Brno University of Technology. Accessed January 15, 2021. http://hdl.handle.net/11012/190398.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Kafka, Roman. “Modelování tepelných účinků elektroporačního procesu na živou tkáň: Modeling of the thermal effects on living tissue during electroporation process.” 2020. Web. 15 Jan 2021.

Vancouver:

Kafka R. Modelování tepelných účinků elektroporačního procesu na živou tkáň: Modeling of the thermal effects on living tissue during electroporation process. [Internet] [Thesis]. Brno University of Technology; 2020. [cited 2021 Jan 15]. Available from: http://hdl.handle.net/11012/190398.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Kafka R. Modelování tepelných účinků elektroporačního procesu na živou tkáň: Modeling of the thermal effects on living tissue during electroporation process. [Thesis]. Brno University of Technology; 2020. Available from: http://hdl.handle.net/11012/190398

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Vrije Universiteit Amsterdam

21. Scheffer, H.J. Lightning strikes: Irreversible electroporation in interventional oncology .

Degree: 2016, Vrije Universiteit Amsterdam

URL: http://hdl.handle.net/1871/54570

Subjects/Keywords: tumor ablation; irreversible electroporation; NanoKnife; interventional radiology; interventional oncology; image-guidance; safety; efficacy

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Scheffer, H. J. (2016). Lightning strikes: Irreversible electroporation in interventional oncology . (Doctoral Dissertation). Vrije Universiteit Amsterdam. Retrieved from http://hdl.handle.net/1871/54570

Chicago Manual of Style (16^th Edition):

Scheffer, H J. “Lightning strikes: Irreversible electroporation in interventional oncology .” 2016. Doctoral Dissertation, Vrije Universiteit Amsterdam. Accessed January 15, 2021. http://hdl.handle.net/1871/54570.

MLA Handbook (7^th Edition):

Scheffer, H J. “Lightning strikes: Irreversible electroporation in interventional oncology .” 2016. Web. 15 Jan 2021.

Vancouver:

Scheffer HJ. Lightning strikes: Irreversible electroporation in interventional oncology . [Internet] [Doctoral dissertation]. Vrije Universiteit Amsterdam; 2016. [cited 2021 Jan 15]. Available from: http://hdl.handle.net/1871/54570.

Council of Science Editors:

Scheffer HJ. Lightning strikes: Irreversible electroporation in interventional oncology . [Doctoral Dissertation]. Vrije Universiteit Amsterdam; 2016. Available from: http://hdl.handle.net/1871/54570

22. Bhonsle, Suyashree P. Non-linearity and Dispersion Effects in Tissue Impedance during Application of High Frequency Electroporation-Inducing Pulsed Electric Fields.

Degree: PhD, Electrical Engineering, 2018, Virginia Tech

URL: http://hdl.handle.net/10919/91904

► Since its conception in 2005, irreversible electroporation (IRE), a non-thermal tumor ablation modality, was investigated for safety and efficacy in clinical applications concerning different organs.… (more)

▼ Since its conception in 2005, irreversible electroporation (IRE), a non-thermal tumor ablation modality, was investigated for safety and efficacy in clinical applications concerning different organs. IRE utilizes high voltage (~3kV), short duration (~100us) pulses to create transient nanoscale defects in the plasma membrane to cause cell death due to irreversible defects, osmotic imbalances and ATP loss. More recently, high-frequency irreversible electroporation (H-FIRE), which employs narrow bipolar pulses (~0.5-10us) delivered in bursts (on time ~100us), was invented to provide benefits such as the mitigation of intense muscle contractions associated with IRE-based treatments. Furthermore, H-FIRE exhibits the potential to improve lesion predictability in homogeneous and heterogeneous tissue masses. Therapeutic IRE and H-FIRE utilize source and sink electrodes inserted into or around the tumor to deliver the treatment. Prediction of the ablation size, for a set of parameters, can be achieved by the use of pre-treatment planning algorithms that calculate the induced electric field distribution in the target tissue. An electric field above a certain threshold induces cell death and parameters are tuned to ensure complete tumor coverage while sparing the nearby healthy tissue. IRE studies have shown that the underlying field is influenced by the increase in tissue conductivity due to enhanced membrane permeability, and treatment outcome can be improved when this nonlinearity is accounted for in numerical models. Since IRE pulses far exceed the time constant of the cell (~1us), the tissue response can be treated as essentially DC a static approximation can be used to predict the field distribution. Alternately, as H-FIRE pulses are on the order of the time constant of the membrane, the tissue response can no longer be treated as DC. The complexity of the H-FIRE-induced field distribution is further enhanced due to the dispersion and non-linearity in biological tissue impedance during treatment. In this dissertation, we have studied the electromagnetic fields induced in tissue during H-FIRE using several experimental and modeling techniques. In addition, we have characterized the nonlinearity and dispersion in tissue impedance during H-FIRE treatments and proposed simpler methods to predict the field distribution to enable easier translation to the clinic. Advisors/Committee Members: Safaai-Jazi, Ahmad (committeechair), Davalos, Rafael V. (committeechair), Zhu, Yizheng (committee member), Verbridge, Scott (committee member), Zhu, Yunhui (committee member).

Subjects/Keywords: Focal Ablation; Irreversible Electroporation; Bipolar Pulses; Dynamic Conductivity

…and modeling of high-frequency irreversible electroporation 33 4.1 Introduction… …high-frequency irreversible electroporation consisting of 1µs and 2µs bursts 61 6.1… …Characterization of conductivity changes during 5µs and 10µs high-frequency irreversible electroporation… …conception in 2005, irreversible electroporation (IRE) has emerged as a promising… …irreversible electroporation (H-FIRE) was proposed to improve the ease of delivery of…

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Bhonsle, S. P. (2018). Non-linearity and Dispersion Effects in Tissue Impedance during Application of High Frequency Electroporation-Inducing Pulsed Electric Fields. (Doctoral Dissertation). Virginia Tech. Retrieved from http://hdl.handle.net/10919/91904

Chicago Manual of Style (16^th Edition):

Bhonsle, Suyashree P. “Non-linearity and Dispersion Effects in Tissue Impedance during Application of High Frequency Electroporation-Inducing Pulsed Electric Fields.” 2018. Doctoral Dissertation, Virginia Tech. Accessed January 15, 2021. http://hdl.handle.net/10919/91904.

MLA Handbook (7^th Edition):

Bhonsle, Suyashree P. “Non-linearity and Dispersion Effects in Tissue Impedance during Application of High Frequency Electroporation-Inducing Pulsed Electric Fields.” 2018. Web. 15 Jan 2021.

Vancouver:

Bhonsle SP. Non-linearity and Dispersion Effects in Tissue Impedance during Application of High Frequency Electroporation-Inducing Pulsed Electric Fields. [Internet] [Doctoral dissertation]. Virginia Tech; 2018. [cited 2021 Jan 15]. Available from: http://hdl.handle.net/10919/91904.

Council of Science Editors:

Bhonsle SP. Non-linearity and Dispersion Effects in Tissue Impedance during Application of High Frequency Electroporation-Inducing Pulsed Electric Fields. [Doctoral Dissertation]. Virginia Tech; 2018. Available from: http://hdl.handle.net/10919/91904

Universitat Pompeu Fabra

23. Qasrawi, Radwan. Treatment planning methods for clinical electroporation.

Degree: Departament de Tecnologies de la Informació i les Comunicacions, 2017, Universitat Pompeu Fabra

URL: http://hdl.handle.net/10803/441753

► Dos modalidades de tratamiento basadas en el fenómeno de la electroporación, la electroquimioterapia y la electroporación irreversible, han sido desarrolladas en las últimas décadas para… (more)

▼ Dos modalidades de tratamiento basadas en el fenómeno de la electroporación, la electroquimioterapia y la electroporación irreversible, han sido desarrolladas en las últimas décadas para destruir tumores sólidos. Estos tratamientos se basan en la aplicación de pulsos cortos de alta tensión a través de electrodos y para su éxito se requiere abarcar todo el tumor con una magnitud de campo eléctrico adecuada. Esto lleva a la necesidad de herramientas software que permitan la planificación de tratamiento específica del paciente. En particular, existe la necesidad de herramientas de planificación de tratamiento similares a las utilizadas en radioterapia para planificar la ubicación de los electrodos y las magnitudes de voltaje a aplicar a través de estos electrodos. Aquí se describe un prototipo de plataforma de planificación de tratamiento que permite a los usuarios realizar la secuencia completa de planificación de tratamiento en un solo entorno. El volumen planificado de tratamiento se representa sobre las imágenes médicas del paciente después de calcular, mediante el método de elementos finitos, la magnitud del campo eléctrico generada por electrodos en forma de aguja. Aquí también se detalla un estudio en el que el prototipo anterior se empleó para analizar el impacto potencial de los vasos sanguíneos hepáticos sobre la ablación de tumores por electroporación irreversible. De este estudio se concluye que estos vasos no deben ser descuidados en la planificación del tratamiento y que alrededor de esos vasos se puede estar produciendo sub-tratamiento frecuentemente en los tratamientos de electroporación irreversible que actualmente se aplican para tumores hepáticos. Finalmente, se describe la implementación y caracterización de un algoritmo semi-analítico rápido para calcular la distribución de campo eléctrico generada por electrodos en forma de aguja. Este algoritmo está destinado a pre-visualizar rápidamente la región de tratamiento esperada antes de proceder con un preciso, pero laborioso y lento, proceso de cálculo basado en métodos numéricos. Advisors/Committee Members: [email protected] (authoremail), true (authoremailshow), Ivorra Cano, Antoni, 1974- (director), Abdeen, Ziad (director), true (authorsendemail).

Subjects/Keywords: Electrochemotherapy; Irreversible electroporation; Treatment planning; Image processing; Segmentation; 3D geometry; Meshing; Decimation; Blood vessels; Liver; Conductivity; Electric field distribution; Numerical modeling; Analytical modeling; Electroquimioteràpia; Electroporación irreversible; Planificación del tratamiento; Procesamiento de imágenes; Segmentación; Geometría 3D; Mallado; Reducción; Vasos sanguíneos; Hígado; Conductividad; Distribución del campo eléctrico; Modelado numérico; Modelado analítico; 62

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Qasrawi, R. (2017). Treatment planning methods for clinical electroporation. (Thesis). Universitat Pompeu Fabra. Retrieved from http://hdl.handle.net/10803/441753

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Qasrawi, Radwan. “Treatment planning methods for clinical electroporation.” 2017. Thesis, Universitat Pompeu Fabra. Accessed January 15, 2021. http://hdl.handle.net/10803/441753.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Qasrawi, Radwan. “Treatment planning methods for clinical electroporation.” 2017. Web. 15 Jan 2021.

Vancouver:

Qasrawi R. Treatment planning methods for clinical electroporation. [Internet] [Thesis]. Universitat Pompeu Fabra; 2017. [cited 2021 Jan 15]. Available from: http://hdl.handle.net/10803/441753.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Qasrawi R. Treatment planning methods for clinical electroporation. [Thesis]. Universitat Pompeu Fabra; 2017. Available from: http://hdl.handle.net/10803/441753

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Universitat Pompeu Fabra

24. Castellví Fernández, Quim. Non-focal non-thermal electrical methods for cancer treatment.

Degree: Departament de Tecnologies de la Informació i les Comunicacions, 2017, Universitat Pompeu Fabra

URL: http://hdl.handle.net/10803/586217

► La majoria del mètodes físics d'ablació tumoral es basen en produir dany tèrmic de manera focalitzada. Tot i ser considerats una alternativa habitual a la… (more)

▼ La majoria del mètodes físics d'ablació tumoral es basen en produir dany tèrmic de manera focalitzada. Tot i ser considerats una alternativa habitual a la resecció quirúrgica, el principi tèrmic de funcionament, comporta un risc per la preservació d'estructures vitals adjacents a la zona de tractament, tals com grans vasos o nervis. A més, el fet de ser focals, fa impracticable la seva aplicació en cas de múltiples nòduls o tumors de difícil accés. Aquesta tesi explora tractaments elèctrics no basats en temperatura, capaços de ser aplicats de manera no focal. S'han investigat dos tractaments: El primer, proposat per altres fa pocs anys, està basat en aplicar permanentment camps elèctrics alterns de baixa magnitud a través d'elèctrodes superficials. Aquí, aquest tractament s'ha estudiat in vivo tant per avaluar la seva eficàcia com per discernir si aquesta resideix en la temperatura. El segon tractament es basa en el fenomen d'electroporació i persegueix el tractament de nòduls hepàtics. En els tractaments basats en electroporació, s’apliquen breus camps elèctrics de gran magnitud per tal de permeabilitzar la membrana cel·lular. Això permet la penetració d’agents quimioterapèutics o produeix directament la mort cel·lular. En lloc d'utilitzar, com és habitual, agulles per tal d'aplicar el tractament, aquí s'explora tractar tot el fetge de forma no localitzada, fent servir grans elèctrodes plans i paral·lels. Utilitzant solucions d'alta conductivitat elèctrica, es pretén magnificar selectivament el camp elèctric sobre els tumors, sent així capaços de destruir tots els tumors i alhora preservar el teixit sà. El tractament proposat per els tumors hepàtics, requereix d'un equip generador actualment no disponible. El presentat treball inclou el disseny d'una nova topologia de generadors capaç de complir amb els requisits. Advisors/Committee Members: [email protected] (authoremail), true (authoremailshow), Ivorra Cano, Antoni, 1974- (director), Bijnens, Bart (director), true (authorsendemail).

Subjects/Keywords: Tractaments elèctrics de càncer; Ablació no tèrmica; Tractament no focals; Electroporació; Electroporació Irreversible; Camps elèctrics alterns; TTFields; Generador modular de polsos; Model de fetge; Conductivitat del teixit; Electrical cancer treatment; Non-thermal ablation; Non-focal treatment; Electroporation; rreversible electroporation; Alternating electric field; TTFields; Modular pulse generator; Liver model; Tissue conductivity; 62

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Castellví Fernández, Q. (2017). Non-focal non-thermal electrical methods for cancer treatment. (Thesis). Universitat Pompeu Fabra. Retrieved from http://hdl.handle.net/10803/586217

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Castellví Fernández, Quim. “Non-focal non-thermal electrical methods for cancer treatment.” 2017. Thesis, Universitat Pompeu Fabra. Accessed January 15, 2021. http://hdl.handle.net/10803/586217.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Castellví Fernández, Quim. “Non-focal non-thermal electrical methods for cancer treatment.” 2017. Web. 15 Jan 2021.

Vancouver:

Castellví Fernández Q. Non-focal non-thermal electrical methods for cancer treatment. [Internet] [Thesis]. Universitat Pompeu Fabra; 2017. [cited 2021 Jan 15]. Available from: http://hdl.handle.net/10803/586217.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Castellví Fernández Q. Non-focal non-thermal electrical methods for cancer treatment. [Thesis]. Universitat Pompeu Fabra; 2017. Available from: http://hdl.handle.net/10803/586217

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Brno University of Technology

25. Puczok, Václav. Zdroj vysokonapěťových pulzů pro elektroporaci buněk: High Voltage Pulse Generator for Electroporation of Cells.

Degree: 2018, Brno University of Technology

URL: http://hdl.handle.net/11012/58837

► The main goal of this thesis is to design control board for the experimental electroporation device and to develop control firmware. The first chapter of… (more)

Subjects/Keywords: ireverzibilní elektroporace; IRE; ablace; nádor; Nanoknife; FPGA; zdroj pulzů; EKG; transformátor; měnič; procesor; LCD; enkodér; paměť EEPROM; stavový automat; irreversible electroporation; IRE; ablation; tumor; Nanoknife; FPGA; pulse source; ECG; transformer; power converter; processor; LCD; encoder; EEPROM memory; finite state machine

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APA · Chicago · MLA · Vancouver · CSE | Export to Zotero / EndNote / Reference Manager

APA (6^th Edition):

Puczok, V. (2018). Zdroj vysokonapěťových pulzů pro elektroporaci buněk: High Voltage Pulse Generator for Electroporation of Cells. (Thesis). Brno University of Technology. Retrieved from http://hdl.handle.net/11012/58837

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Chicago Manual of Style (16^th Edition):

Puczok, Václav. “Zdroj vysokonapěťových pulzů pro elektroporaci buněk: High Voltage Pulse Generator for Electroporation of Cells.” 2018. Thesis, Brno University of Technology. Accessed January 15, 2021. http://hdl.handle.net/11012/58837.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

MLA Handbook (7^th Edition):

Puczok, Václav. “Zdroj vysokonapěťových pulzů pro elektroporaci buněk: High Voltage Pulse Generator for Electroporation of Cells.” 2018. Web. 15 Jan 2021.

Vancouver:

Puczok V. Zdroj vysokonapěťových pulzů pro elektroporaci buněk: High Voltage Pulse Generator for Electroporation of Cells. [Internet] [Thesis]. Brno University of Technology; 2018. [cited 2021 Jan 15]. Available from: http://hdl.handle.net/11012/58837.

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

Council of Science Editors:

Puczok V. Zdroj vysokonapěťových pulzů pro elektroporaci buněk: High Voltage Pulse Generator for Electroporation of Cells. [Thesis]. Brno University of Technology; 2018. Available from: http://hdl.handle.net/11012/58837

Note: this citation may be lacking information needed for this citation format:
Not specified: Masters Thesis or Doctoral Dissertation

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Open Access Theses and Dissertations